PI0002897 (gene) Melon (PI 482460) v1

Overview
NamePI0002897
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptiondelta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal
Locationchr07: 2429830 .. 2431362 (-)
RNA-Seq ExpressionPI0002897
SyntenyPI0002897
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAATCGAGGAGGTGCTTCTTTTGAGTTTTGGATTCCCAAGATATCTCAGACCTTGAGACCACACGTATATGTCTTAAAACATAATTCTTTTTTGGACTATATTTGGATATTTGGACTTCAAATCCACACCCACCTTTACACAAAAGTCCACTTCTCAAAGTATATTTCCAATTCTTCAAAATTATAACCGACCACTCTCCGCCGAAGGCAAATGGAAGGCTACAAATGCATAAAAATCGAGCGTCAAAACCCAAACTCCGGCGTCTTCCACATCCGCCTCCACCGTCCATCACACTACAACGCTCTCACAACCCAACTCTTCACCGAACTCCGTCAAGCCTTCTCACATCTAGACCAAAACCCCGACGTCCACGTCATCATCCTTTCCGGATCCGGCAAACACTTCTGCGCCGGAATCGACCTCAAATCGACAGCCTCCAACTTCGAGAAGCATCTATCGGAGGAACGGGGACGAGCAGGGGAAAGAATCCGACGGGAAATCAAATGGATGCAAGAGTCAATCACGGCTATCGAGGAGTGCCGGAAGCCGGTGATAGCAAGCATCCACGGAGGGTGCATCGGAGGAGGAATTGATATAGTGACGGCTTGTGATTTGAGGTATTGCACGGCGGAGGCGGTGTTTTCGGTGAGGGAGGTTAAAATGGCGATTACGGCGGATCTTGGGACTTTACAGAGACTGCCAAGAATCGTAGGGTATGGGAAGGCGGCGGAGTTGGCGTTGACGGGCCGAGATTTTTCGGGCTTGGAGGCTAAGGAATTGGGCTTGGTTTCTCGAACTTTTGATTCCATATCGGAGCTTCAAGATGGAGTCCTCAAAATTGCTCAAGGTATCAAATTCTCTCTATTTACCAATTTTCCTTTGCCTTTTATTTTTAAAATTTTCTTTTTAAATACACGACACTTGATTTTGCTAAGATATTTTGCCAACTTTACTATGATAAATTCAGTTATGTTTCTAGTATTCTTGTATGTGCATCTAAATTATTTAATAAAAAATGAAAGCTTGCAAGCATAGGATTGTTGTGTATTGTGTATGAGATTGGGAAAAAGGAATGAAAAGTGGATGGTGAGTGCAGAAATAGCATCAAGATCTCCATTGGCTGTGGTGGGAACAAAGGCAGTGCTACTAAAAAGTAGAGACCTAAATGTAGAACAAGGGTTAGATTATGTTGCAACTTGGAACTCAGGGACTCTACTCTCGGATGATCTCAAAGAAGCCATCTCCGCTCAAGCTAACAAACGCCAACCGAAGTTTTCCAAGCTTTAAGACGGAGAGACGTGAACATTTGATTTCTTGACCAAGAGAGCATATATATGCTTCTAACTTCTTGAACTATATTCAAGTTAGTTTTTGGTCTCAAAACAGCTACATCCAACATTTTACTTTTGGCTTTTTCCTCTCGGTAATTACATGAAATACCATCGTGTATGTGCTTATCCTTTTCTCGACCACCTTTGGTTTTTCTTGTTCTTAATAAAAAGAAAATGGTAAACATATATTTTAATCTTCAA

mRNA sequence

CAATCGAGGAGGTGCTTCTTTTGAGTTTTGGATTCCCAAGATATCTCAGACCTTGAGACCACACGTATATGTCTTAAAACATAATTCTTTTTTGGACTATATTTGGATATTTGGACTTCAAATCCACACCCACCTTTACACAAAAGTCCACTTCTCAAAGTATATTTCCAATTCTTCAAAATTATAACCGACCACTCTCCGCCGAAGGCAAATGGAAGGCTACAAATGCATAAAAATCGAGCGTCAAAACCCAAACTCCGGCGTCTTCCACATCCGCCTCCACCGTCCATCACACTACAACGCTCTCACAACCCAACTCTTCACCGAACTCCGTCAAGCCTTCTCACATCTAGACCAAAACCCCGACGTCCACGTCATCATCCTTTCCGGATCCGGCAAACACTTCTGCGCCGGAATCGACCTCAAATCGACAGCCTCCAACTTCGAGAAGCATCTATCGGAGGAACGGGGACGAGCAGGGGAAAGAATCCGACGGGAAATCAAATGGATGCAAGAGTCAATCACGGCTATCGAGGAGTGCCGGAAGCCGGTGATAGCAAGCATCCACGGAGGGTGCATCGGAGGAGGAATTGATATAGTGACGGCTTGTGATTTGAGGTATTGCACGGCGGAGGCGGTGTTTTCGGTGAGGGAGGTTAAAATGGCGATTACGGCGGATCTTGGGACTTTACAGAGACTGCCAAGAATCGTAGGGTATGGGAAGGCGGCGGAGTTGGCGTTGACGGGCCGAGATTTTTCGGGCTTGGAGGCTAAGGAATTGGGCTTGGTTTCTCGAACTTTTGATTCCATATCGGAGCTTCAAGATGGAGTCCTCAAAATTGCTCAAGAAATAGCATCAAGATCTCCATTGGCTGTGGTGGGAACAAAGGCAGTGCTACTAAAAAGTAGAGACCTAAATGTAGAACAAGGGTTAGATTATGTTGCAACTTGGAACTCAGGGACTCTACTCTCGGATGATCTCAAAGAAGCCATCTCCGCTCAAGCTAACAAACGCCAACCGAAGTTTTCCAAGCTTTAAGACGGAGAGACGTGAACATTTGATTTCTTGACCAAGAGAGCATATATATGCTTCTAACTTCTTGAACTATATTCAAGTTAGTTTTTGGTCTCAAAACAGCTACATCCAACATTTTACTTTTGGCTTTTTCCTCTCGGTAATTACATGAAATACCATCGTGTATGTGCTTATCCTTTTCTCGACCACCTTTGGTTTTTCTTGTTCTTAATAAAAAGAAAATGGTAAACATATATTTTAATCTTCAA

Coding sequence (CDS)

ATGGAAGGCTACAAATGCATAAAAATCGAGCGTCAAAACCCAAACTCCGGCGTCTTCCACATCCGCCTCCACCGTCCATCACACTACAACGCTCTCACAACCCAACTCTTCACCGAACTCCGTCAAGCCTTCTCACATCTAGACCAAAACCCCGACGTCCACGTCATCATCCTTTCCGGATCCGGCAAACACTTCTGCGCCGGAATCGACCTCAAATCGACAGCCTCCAACTTCGAGAAGCATCTATCGGAGGAACGGGGACGAGCAGGGGAAAGAATCCGACGGGAAATCAAATGGATGCAAGAGTCAATCACGGCTATCGAGGAGTGCCGGAAGCCGGTGATAGCAAGCATCCACGGAGGGTGCATCGGAGGAGGAATTGATATAGTGACGGCTTGTGATTTGAGGTATTGCACGGCGGAGGCGGTGTTTTCGGTGAGGGAGGTTAAAATGGCGATTACGGCGGATCTTGGGACTTTACAGAGACTGCCAAGAATCGTAGGGTATGGGAAGGCGGCGGAGTTGGCGTTGACGGGCCGAGATTTTTCGGGCTTGGAGGCTAAGGAATTGGGCTTGGTTTCTCGAACTTTTGATTCCATATCGGAGCTTCAAGATGGAGTCCTCAAAATTGCTCAAGAAATAGCATCAAGATCTCCATTGGCTGTGGTGGGAACAAAGGCAGTGCTACTAAAAAGTAGAGACCTAAATGTAGAACAAGGGTTAGATTATGTTGCAACTTGGAACTCAGGGACTCTACTCTCGGATGATCTCAAAGAAGCCATCTCCGCTCAAGCTAACAAACGCCAACCGAAGTTTTCCAAGCTTTAA

Protein sequence

MEGYKCIKIERQNPNSGVFHIRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSGSGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHGGCIGGGIDIVTACDLRYCTAEAVFSVREVKMAITADLGTLQRLPRIVGYGKAAELALTGRDFSGLEAKELGLVSRTFDSISELQDGVLKIAQEIASRSPLAVVGTKAVLLKSRDLNVEQGLDYVATWNSGTLLSDDLKEAISAQANKRQPKFSKL
Homology
BLAST of PI0002897 vs. ExPASy Swiss-Prot
Match: Q9FHR8 (Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=DCI1 PE=1 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 5.3e-97
Identity = 176/276 (63.77%), Postives = 221/276 (80.07%), Query Frame = 0

Query: 1   MEGYKCIKIERQNPNSGVFHIRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60
           ME YK ++I R+N +S VFH+ ++RPSH NAL+   F E  +A S LDQNPDV VIILSG
Sbjct: 3   MESYKTLEIIRKNTDSSVFHLIINRPSHLNALSLDFFIEFPKALSSLDQNPDVSVIILSG 62

Query: 61  SGKHFCAGIDLKSTAS-NFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIH 120
           +GKHFC+GIDL S +S + +     +RGR+ E++RR+IK MQ +ITAIE+CRKPVIA+IH
Sbjct: 63  AGKHFCSGIDLNSLSSISTQSSSGNDRGRSSEQLRRKIKSMQAAITAIEQCRKPVIAAIH 122

Query: 121 GGCIGGGIDIVTACDLRYCTAEAVFSVREVKMAITADLGTLQRLPRIVGYGKAAELALTG 180
           G CIGGG+D++TACD+RYC+ +A FS++EV +AI ADLGTLQRLP IVGY  A ELALT 
Sbjct: 123 GACIGGGVDLITACDIRYCSEDAFFSIKEVDLAIVADLGTLQRLPSIVGYANAMELALTA 182

Query: 181 RDFSGLEAKELGLVSRTFDSISELQDGVLKIAQEIASRSPLAVVGTKAVLLKSRDLNVEQ 240
           R FSG EAK+LGLVS+ F S SEL +GV  IA+ I  +SPLAV GTKAVLL+SR+++VEQ
Sbjct: 183 RRFSGSEAKDLGLVSKVFGSKSELDNGVTTIAEGIGGKSPLAVTGTKAVLLRSREVSVEQ 242

Query: 241 GLDYVATWNSGTLLSDDLKEAISAQANKRQPKFSKL 276
           GLDYVATWNS  L+SDDL EA+SAQ  KR+P+F+KL
Sbjct: 243 GLDYVATWNSAMLISDDLNEAVSAQMMKRKPRFAKL 278

BLAST of PI0002897 vs. ExPASy Swiss-Prot
Match: O35459 (Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial OS=Mus musculus OX=10090 GN=Ech1 PE=1 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 3.8e-55
Identity = 110/262 (41.98%), Postives = 164/262 (62.60%), Query Frame = 0

Query: 18  VFHIRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSGSGKHFCAGIDLKSTASN 77
           V H++L+RP   NA+    + EL + F  + ++ D   +++SG+GK F +GIDL   AS 
Sbjct: 66  VLHVQLNRPEKRNAMNRAFWRELVECFQKISKDSDCRAVVVSGAGKMFTSGIDLMDMASE 125

Query: 78  FEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHGGCIGGGIDIVTACDLRY 137
             +   ++  R    +R  I   Q++ T IE+C KPVIA+IHGGCIGGG+D+V+ACD+RY
Sbjct: 126 LMQPSGDDAARIAWYLRDLISKYQKTFTVIEKCPKPVIAAIHGGCIGGGVDLVSACDIRY 185

Query: 138 CTAEAVFSVREVKMAITADLGTLQRLPRIVG-YGKAAELALTGRDFSGLEAKELGLVSRT 197
           CT +A F ++EV M + AD+GTLQRLP+++G      EL  + R     EA + GLVSR 
Sbjct: 186 CTQDAFFQIKEVDMGLAADVGTLQRLPKVIGNQSLVNELTFSARKMMADEALDSGLVSRV 245

Query: 198 FDSISELQDGVLKIAQEIASRSPLAVVGTKAVLLKSRDLNVEQGLDYVATWNSGTLLSDD 257
           F     + +    +A +I+S+SP+AV G+K  L+ SRD +V++ LDY+ATWN   L + D
Sbjct: 246 FQDKDAMLNAAFALAADISSKSPVAVQGSKINLIYSRDHSVDESLDYMATWNMSMLQTQD 305

Query: 258 LKEAISAQANKRQPK---FSKL 276
           + +++ A   KR  K   FSKL
Sbjct: 306 IIKSVQAAMEKRDTKSITFSKL 327

BLAST of PI0002897 vs. ExPASy Swiss-Prot
Match: Q5RFG0 (Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial OS=Pongo abelii OX=9601 GN=ECH1 PE=2 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 1.1e-54
Identity = 112/262 (42.75%), Postives = 164/262 (62.60%), Query Frame = 0

Query: 18  VFHIRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSGSGKHFCAGIDLKSTASN 77
           V H++L+RP+  NA+    + E+ + F+ + ++ D   +++SG+GK F AG+DL   AS+
Sbjct: 67  VLHVQLNRPNKRNAMNKVFWREMVECFNKISRDADCRAVVISGAGKMFTAGVDLMDMASD 126

Query: 78  FEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHGGCIGGGIDIVTACDLRY 137
             +   ++  R    +R  I   QE+   IE+C KPVIA++HGGCIGGG+D+VTACD+RY
Sbjct: 127 ILQPKGDDVARISWYLRDIITRYQETFNVIEKCPKPVIAAVHGGCIGGGVDLVTACDIRY 186

Query: 138 CTAEAVFSVREVKMAITADLGTLQRLPRIVG-YGKAAELALTGRDFSGLEAKELGLVSRT 197
           C  +A F V+EV + + AD+GTLQRLP+++G      ELA T R     EA   GLVSR 
Sbjct: 187 CAQDAFFQVKEVDVGLAADVGTLQRLPKVIGNQSLVNELAFTARKMMADEALGSGLVSRV 246

Query: 198 FDSISELQDGVLKIAQEIASRSPLAVVGTKAVLLKSRDLNVEQGLDYVATWNSGTLLSDD 257
           F     + D  L +A EI+S+SP+AV  TK  LL SRD +V + L+YVA+WN   L + D
Sbjct: 247 FPDKEVMLDAALALAAEISSKSPVAVQSTKVNLLYSRDHSVAESLNYVASWNMSMLQTQD 306

Query: 258 LKEAISAQANKRQPK---FSKL 276
           L +++ A    ++ K   FSKL
Sbjct: 307 LMKSVQAATENKELKSVTFSKL 328

BLAST of PI0002897 vs. ExPASy Swiss-Prot
Match: Q13011 (Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial OS=Homo sapiens OX=9606 GN=ECH1 PE=1 SV=2)

HSP 1 Score: 214.5 bits (545), Expect = 1.5e-54
Identity = 113/262 (43.13%), Postives = 163/262 (62.21%), Query Frame = 0

Query: 18  VFHIRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSGSGKHFCAGIDLKSTASN 77
           V H++L+RP+  NA+    + E+ + F+ + ++ D   +++SG+GK F AGIDL   AS+
Sbjct: 67  VLHVQLNRPNKRNAMNKVFWREMVECFNKISRDADCRAVVISGAGKMFTAGIDLMDMASD 126

Query: 78  FEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHGGCIGGGIDIVTACDLRY 137
             +   ++  R    +R  I   QE+   IE C KPVIA++HGGCIGGG+D+VTACD+RY
Sbjct: 127 ILQPKGDDVARISWYLRDIITRYQETFNVIERCPKPVIAAVHGGCIGGGVDLVTACDIRY 186

Query: 138 CTAEAVFSVREVKMAITADLGTLQRLPRIVG-YGKAAELALTGRDFSGLEAKELGLVSRT 197
           C  +A F V+EV + + AD+GTLQRLP+++G      ELA T R     EA   GLVSR 
Sbjct: 187 CAQDAFFQVKEVDVGLAADVGTLQRLPKVIGNQSLVNELAFTARKMMADEALGSGLVSRV 246

Query: 198 FDSISELQDGVLKIAQEIASRSPLAVVGTKAVLLKSRDLNVEQGLDYVATWNSGTLLSDD 257
           F     + D  L +A EI+S+SP+AV  TK  LL SRD +V + L+YVA+WN   L + D
Sbjct: 247 FPDKEVMLDAALALAAEISSKSPVAVQSTKVNLLYSRDHSVAESLNYVASWNMSMLQTQD 306

Query: 258 LKEAISAQANKRQPK---FSKL 276
           L +++ A    ++ K   FSKL
Sbjct: 307 LVKSVQATTENKELKTVTFSKL 328

BLAST of PI0002897 vs. ExPASy Swiss-Prot
Match: Q62651 (Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial OS=Rattus norvegicus OX=10116 GN=Ech1 PE=1 SV=2)

HSP 1 Score: 213.4 bits (542), Expect = 3.3e-54
Identity = 109/262 (41.60%), Postives = 165/262 (62.98%), Query Frame = 0

Query: 18  VFHIRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSGSGKHFCAGIDLKSTASN 77
           V H++L+RP   NA+    + EL + F  + ++ D   +++SG+GK F +GIDL   AS+
Sbjct: 66  VLHVQLNRPEKRNAMNRAFWRELVECFQKISKDSDCRAVVVSGAGKMFTSGIDLMDMASD 125

Query: 78  FEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHGGCIGGGIDIVTACDLRY 137
             +   ++  R    +R  I   Q++ T IE+C KPVIA+IHGGCIGGG+D+++ACD+RY
Sbjct: 126 ILQPPGDDVARIAWYLRDLISRYQKTFTVIEKCPKPVIAAIHGGCIGGGVDLISACDIRY 185

Query: 138 CTAEAVFSVREVKMAITADLGTLQRLPRIVGYGKAA-ELALTGRDFSGLEAKELGLVSRT 197
           CT +A F V+EV + + AD+GTLQRLP+++G      EL  T R     EA + GLVSR 
Sbjct: 186 CTQDAFFQVKEVDVGLAADVGTLQRLPKVIGNRSLVNELTFTARKMMADEALDSGLVSRV 245

Query: 198 FDSISELQDGVLKIAQEIASRSPLAVVGTKAVLLKSRDLNVEQGLDYVATWNSGTLLSDD 257
           F     + +    +A +I+S+SP+AV G+K  L+ SRD +V++ LDY+ATWN   L + D
Sbjct: 246 FPDKDVMLNAAFALAADISSKSPVAVQGSKINLIYSRDHSVDESLDYMATWNMSMLQTQD 305

Query: 258 LKEAISAQANKRQPK---FSKL 276
           + +++ A   K+  K   FSKL
Sbjct: 306 IIKSVQAAMEKKDSKSITFSKL 327

BLAST of PI0002897 vs. ExPASy TrEMBL
Match: A0A5D3D1B6 (Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G001700 PE=3 SV=1)

HSP 1 Score: 531.9 bits (1369), Expect = 1.5e-147
Identity = 267/275 (97.09%), Postives = 274/275 (99.64%), Query Frame = 0

Query: 1   MEGYKCIKIERQNPNSGVFHIRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60
           MEGY+CIKIERQ+PNSGVFH+RLHRPSHYNALTTQLFTELRQAFS LDQNPDVHVIILSG
Sbjct: 1   MEGYECIKIERQSPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSRLDQNPDVHVIILSG 60

Query: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120
           SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG
Sbjct: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120

Query: 121 GCIGGGIDIVTACDLRYCTAEAVFSVREVKMAITADLGTLQRLPRIVGYGKAAELALTGR 180
           GCIGGGIDIVTACDLRYCTAEAVFSVREVK+AITADLGTLQRLPRIVGYGKAAELALTGR
Sbjct: 121 GCIGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR 180

Query: 181 DFSGLEAKELGLVSRTFDSISELQDGVLKIAQEIASRSPLAVVGTKAVLLKSRDLNVEQG 240
           DFSGLEAKELGLVSRTFDS+SELQDGVLKIA+EIASRSPLAVVGTKAVLLKSRDLNVEQG
Sbjct: 181 DFSGLEAKELGLVSRTFDSMSELQDGVLKIAEEIASRSPLAVVGTKAVLLKSRDLNVEQG 240

Query: 241 LDYVATWNSGTLLSDDLKEAISAQANKRQPKFSKL 276
           LDYVATWNSGTLLSDDLKEAISAQANKR+PKFSKL
Sbjct: 241 LDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL 275

BLAST of PI0002897 vs. ExPASy TrEMBL
Match: A0A1S3BXS5 (delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103494575 PE=3 SV=1)

HSP 1 Score: 531.9 bits (1369), Expect = 1.5e-147
Identity = 267/275 (97.09%), Postives = 274/275 (99.64%), Query Frame = 0

Query: 1   MEGYKCIKIERQNPNSGVFHIRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60
           MEGY+CIKIERQ+PNSGVFH+RLHRPSHYNALTTQLFTELRQAFS LDQNPDVHVIILSG
Sbjct: 1   MEGYECIKIERQSPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSRLDQNPDVHVIILSG 60

Query: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120
           SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG
Sbjct: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120

Query: 121 GCIGGGIDIVTACDLRYCTAEAVFSVREVKMAITADLGTLQRLPRIVGYGKAAELALTGR 180
           GCIGGGIDIVTACDLRYCTAEAVFSVREVK+AITADLGTLQRLPRIVGYGKAAELALTGR
Sbjct: 121 GCIGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR 180

Query: 181 DFSGLEAKELGLVSRTFDSISELQDGVLKIAQEIASRSPLAVVGTKAVLLKSRDLNVEQG 240
           DFSGLEAKELGLVSRTFDS+SELQDGVLKIA+EIASRSPLAVVGTKAVLLKSRDLNVEQG
Sbjct: 181 DFSGLEAKELGLVSRTFDSMSELQDGVLKIAEEIASRSPLAVVGTKAVLLKSRDLNVEQG 240

Query: 241 LDYVATWNSGTLLSDDLKEAISAQANKRQPKFSKL 276
           LDYVATWNSGTLLSDDLKEAISAQANKR+PKFSKL
Sbjct: 241 LDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL 275

BLAST of PI0002897 vs. ExPASy TrEMBL
Match: A0A5A7TMZ4 (Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G003090 PE=3 SV=1)

HSP 1 Score: 528.9 bits (1361), Expect = 1.3e-146
Identity = 266/275 (96.73%), Postives = 273/275 (99.27%), Query Frame = 0

Query: 1   MEGYKCIKIERQNPNSGVFHIRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60
           MEGY+CIKIERQ+PNSGVFH+RLHRPSHYNALTTQLFTELRQAFS LDQNPDVHVIILSG
Sbjct: 1   MEGYECIKIERQSPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSRLDQNPDVHVIILSG 60

Query: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120
           SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG
Sbjct: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120

Query: 121 GCIGGGIDIVTACDLRYCTAEAVFSVREVKMAITADLGTLQRLPRIVGYGKAAELALTGR 180
           GCIGGGIDIVTACDLRYCTAEAVFSVREVK+AITADLGTLQRLPRIVGYGKAAELALTGR
Sbjct: 121 GCIGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR 180

Query: 181 DFSGLEAKELGLVSRTFDSISELQDGVLKIAQEIASRSPLAVVGTKAVLLKSRDLNVEQG 240
           DFSGLEAKELGLVSRTFDS+SELQDGVLKIA+EIASRSPLAVVGTKAVLLKSRDL VEQG
Sbjct: 181 DFSGLEAKELGLVSRTFDSMSELQDGVLKIAEEIASRSPLAVVGTKAVLLKSRDLYVEQG 240

Query: 241 LDYVATWNSGTLLSDDLKEAISAQANKRQPKFSKL 276
           LDYVATWNSGTLLSDDLKEAISAQANKR+PKFSKL
Sbjct: 241 LDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL 275

BLAST of PI0002897 vs. ExPASy TrEMBL
Match: A0A0A0KXB8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G011790 PE=3 SV=1)

HSP 1 Score: 528.9 bits (1361), Expect = 1.3e-146
Identity = 265/275 (96.36%), Postives = 273/275 (99.27%), Query Frame = 0

Query: 1   MEGYKCIKIERQNPNSGVFHIRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60
           ME YKCIKIERQNPNSGVFH+RLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG
Sbjct: 1   MEDYKCIKIERQNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60

Query: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120
           SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG
Sbjct: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120

Query: 121 GCIGGGIDIVTACDLRYCTAEAVFSVREVKMAITADLGTLQRLPRIVGYGKAAELALTGR 180
           GC+GGGIDIVTACDLRYCTAEAVFSVREVK+AITADLGTLQRLPRIVGYGKAAELALTGR
Sbjct: 121 GCVGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR 180

Query: 181 DFSGLEAKELGLVSRTFDSISELQDGVLKIAQEIASRSPLAVVGTKAVLLKSRDLNVEQG 240
           +FSGLEAKELGLVSRTFDSIS+LQDGVLKIA+EIAS SPLAVVGTKAVLLKSRDLNVEQG
Sbjct: 181 NFSGLEAKELGLVSRTFDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQG 240

Query: 241 LDYVATWNSGTLLSDDLKEAISAQANKRQPKFSKL 276
           LDYVATWNSGTLLSDDLKEAISAQA+KR+PKFSKL
Sbjct: 241 LDYVATWNSGTLLSDDLKEAISAQASKRKPKFSKL 275

BLAST of PI0002897 vs. ExPASy TrEMBL
Match: A0A6J1GW60 (delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111458038 PE=3 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 7.4e-126
Identity = 232/275 (84.36%), Postives = 250/275 (90.91%), Query Frame = 0

Query: 1   MEGYKCIKIERQNPNSGVFHIRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60
           ME YK IKIERQNPNSGVFH+RLHRPSHYNALTT+LF ELR+A S LDQNPDV VIILSG
Sbjct: 1   MEDYKSIKIERQNPNSGVFHLRLHRPSHYNALTTELFAELRRALSSLDQNPDVRVIILSG 60

Query: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120
           SGKHFCAGIDLKSTASNFE  LS ERGRAGER+RRE+K MQ+SITAIEECRKPVIA IHG
Sbjct: 61  SGKHFCAGIDLKSTASNFEDLLSSERGRAGERLRREVKAMQDSITAIEECRKPVIACIHG 120

Query: 121 GCIGGGIDIVTACDLRYCTAEAVFSVREVKMAITADLGTLQRLPRIVGYGKAAELALTGR 180
           GCIGGG+DI TACDLRYCTAEAVFSV+E+ +AITADLGTLQRLP IVG+GKA ELALTGR
Sbjct: 121 GCIGGGVDIATACDLRYCTAEAVFSVKEINLAITADLGTLQRLPGIVGFGKAVELALTGR 180

Query: 181 DFSGLEAKELGLVSRTFDSISELQDGVLKIAQEIASRSPLAVVGTKAVLLKSRDLNVEQG 240
           +FSG EAKELGLVS TF S  EL +GV+KIA+EIAS+SPLAVVGTKAVLLKSRDL VEQG
Sbjct: 181 EFSGFEAKELGLVSTTFASKMELDNGVIKIAEEIASKSPLAVVGTKAVLLKSRDLTVEQG 240

Query: 241 LDYVATWNSGTLLSDDLKEAISAQANKRQPKFSKL 276
           LDYVATWNSGTLLS DLKEAISA+A+KR P FSKL
Sbjct: 241 LDYVATWNSGTLLSADLKEAISARAHKRNPTFSKL 275

BLAST of PI0002897 vs. NCBI nr
Match: XP_008454026.1 (PREDICTED: delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal [Cucumis melo] >TYK17024.1 delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal [Cucumis melo var. makuwa])

HSP 1 Score: 531.9 bits (1369), Expect = 3.2e-147
Identity = 267/275 (97.09%), Postives = 274/275 (99.64%), Query Frame = 0

Query: 1   MEGYKCIKIERQNPNSGVFHIRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60
           MEGY+CIKIERQ+PNSGVFH+RLHRPSHYNALTTQLFTELRQAFS LDQNPDVHVIILSG
Sbjct: 1   MEGYECIKIERQSPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSRLDQNPDVHVIILSG 60

Query: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120
           SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG
Sbjct: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120

Query: 121 GCIGGGIDIVTACDLRYCTAEAVFSVREVKMAITADLGTLQRLPRIVGYGKAAELALTGR 180
           GCIGGGIDIVTACDLRYCTAEAVFSVREVK+AITADLGTLQRLPRIVGYGKAAELALTGR
Sbjct: 121 GCIGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR 180

Query: 181 DFSGLEAKELGLVSRTFDSISELQDGVLKIAQEIASRSPLAVVGTKAVLLKSRDLNVEQG 240
           DFSGLEAKELGLVSRTFDS+SELQDGVLKIA+EIASRSPLAVVGTKAVLLKSRDLNVEQG
Sbjct: 181 DFSGLEAKELGLVSRTFDSMSELQDGVLKIAEEIASRSPLAVVGTKAVLLKSRDLNVEQG 240

Query: 241 LDYVATWNSGTLLSDDLKEAISAQANKRQPKFSKL 276
           LDYVATWNSGTLLSDDLKEAISAQANKR+PKFSKL
Sbjct: 241 LDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL 275

BLAST of PI0002897 vs. NCBI nr
Match: XP_004152122.1 (delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal [Cucumis sativus] >KGN53032.1 hypothetical protein Csa_014540 [Cucumis sativus])

HSP 1 Score: 528.9 bits (1361), Expect = 2.7e-146
Identity = 265/275 (96.36%), Postives = 273/275 (99.27%), Query Frame = 0

Query: 1   MEGYKCIKIERQNPNSGVFHIRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60
           ME YKCIKIERQNPNSGVFH+RLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG
Sbjct: 1   MEDYKCIKIERQNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60

Query: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120
           SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG
Sbjct: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120

Query: 121 GCIGGGIDIVTACDLRYCTAEAVFSVREVKMAITADLGTLQRLPRIVGYGKAAELALTGR 180
           GC+GGGIDIVTACDLRYCTAEAVFSVREVK+AITADLGTLQRLPRIVGYGKAAELALTGR
Sbjct: 121 GCVGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR 180

Query: 181 DFSGLEAKELGLVSRTFDSISELQDGVLKIAQEIASRSPLAVVGTKAVLLKSRDLNVEQG 240
           +FSGLEAKELGLVSRTFDSIS+LQDGVLKIA+EIAS SPLAVVGTKAVLLKSRDLNVEQG
Sbjct: 181 NFSGLEAKELGLVSRTFDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQG 240

Query: 241 LDYVATWNSGTLLSDDLKEAISAQANKRQPKFSKL 276
           LDYVATWNSGTLLSDDLKEAISAQA+KR+PKFSKL
Sbjct: 241 LDYVATWNSGTLLSDDLKEAISAQASKRKPKFSKL 275

BLAST of PI0002897 vs. NCBI nr
Match: KAA0044560.1 (delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal [Cucumis melo var. makuwa])

HSP 1 Score: 528.9 bits (1361), Expect = 2.7e-146
Identity = 266/275 (96.73%), Postives = 273/275 (99.27%), Query Frame = 0

Query: 1   MEGYKCIKIERQNPNSGVFHIRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60
           MEGY+CIKIERQ+PNSGVFH+RLHRPSHYNALTTQLFTELRQAFS LDQNPDVHVIILSG
Sbjct: 1   MEGYECIKIERQSPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSRLDQNPDVHVIILSG 60

Query: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120
           SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG
Sbjct: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120

Query: 121 GCIGGGIDIVTACDLRYCTAEAVFSVREVKMAITADLGTLQRLPRIVGYGKAAELALTGR 180
           GCIGGGIDIVTACDLRYCTAEAVFSVREVK+AITADLGTLQRLPRIVGYGKAAELALTGR
Sbjct: 121 GCIGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR 180

Query: 181 DFSGLEAKELGLVSRTFDSISELQDGVLKIAQEIASRSPLAVVGTKAVLLKSRDLNVEQG 240
           DFSGLEAKELGLVSRTFDS+SELQDGVLKIA+EIASRSPLAVVGTKAVLLKSRDL VEQG
Sbjct: 181 DFSGLEAKELGLVSRTFDSMSELQDGVLKIAEEIASRSPLAVVGTKAVLLKSRDLYVEQG 240

Query: 241 LDYVATWNSGTLLSDDLKEAISAQANKRQPKFSKL 276
           LDYVATWNSGTLLSDDLKEAISAQANKR+PKFSKL
Sbjct: 241 LDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL 275

BLAST of PI0002897 vs. NCBI nr
Match: XP_038879593.1 (delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal isoform X1 [Benincasa hispida])

HSP 1 Score: 503.1 bits (1294), Expect = 1.6e-138
Identity = 253/275 (92.00%), Postives = 264/275 (96.00%), Query Frame = 0

Query: 1   MEGYKCIKIERQNPNSGVFHIRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60
           MEGYKCIKI+RQNPNSGVFH+RLHRPSH+NALTT+LF ELRQAFS+LDQNP+VHVIILSG
Sbjct: 1   MEGYKCIKIDRQNPNSGVFHLRLHRPSHHNALTTELFAELRQAFSYLDQNPEVHVIILSG 60

Query: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120
           SGKHFCAGIDLKSTASNF K  SEERGRAGER+RREIKWMQESITAIEECRKPVIASIHG
Sbjct: 61  SGKHFCAGIDLKSTASNFSKLQSEERGRAGERLRREIKWMQESITAIEECRKPVIASIHG 120

Query: 121 GCIGGGIDIVTACDLRYCTAEAVFSVREVKMAITADLGTLQRLPRIVGYGKAAELALTGR 180
           GCIGGG+DIVTACDLRYCTAEAVFSVREV+MAITADLGTLQRLPRIVGYGKAAELALTGR
Sbjct: 121 GCIGGGVDIVTACDLRYCTAEAVFSVREVRMAITADLGTLQRLPRIVGYGKAAELALTGR 180

Query: 181 DFSGLEAKELGLVSRTFDSISELQDGVLKIAQEIASRSPLAVVGTKAVLLKSRDLNVEQG 240
           DFSGLEAKELGLVSRTF S  EL+D VLKIAQEI S+SPLAVVGTKAVLLKSRDLNVEQG
Sbjct: 181 DFSGLEAKELGLVSRTFASKLELEDEVLKIAQEIGSKSPLAVVGTKAVLLKSRDLNVEQG 240

Query: 241 LDYVATWNSGTLLSDDLKEAISAQANKRQPKFSKL 276
           LDYVATWNSGTLLSDDLKEAISAQANKR P FSKL
Sbjct: 241 LDYVATWNSGTLLSDDLKEAISAQANKRNPVFSKL 275

BLAST of PI0002897 vs. NCBI nr
Match: XP_023526792.1 (delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal [Cucurbita pepo subsp. pepo])

HSP 1 Score: 460.3 bits (1183), Expect = 1.2e-125
Identity = 233/275 (84.73%), Postives = 251/275 (91.27%), Query Frame = 0

Query: 1   MEGYKCIKIERQNPNSGVFHIRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60
           ME YK IKIERQNP+SGVFH+RLHRPSHYNALTT+LF ELR+A S LDQNPDV VIILSG
Sbjct: 1   MEDYKSIKIERQNPSSGVFHLRLHRPSHYNALTTELFAELRRALSSLDQNPDVRVIILSG 60

Query: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120
           SGKHFCAGIDLKSTASNFE  LS ERGRAGER+RRE+K MQ+SITAIEECRKPVIA IHG
Sbjct: 61  SGKHFCAGIDLKSTASNFENLLSSERGRAGERLRREVKAMQDSITAIEECRKPVIACIHG 120

Query: 121 GCIGGGIDIVTACDLRYCTAEAVFSVREVKMAITADLGTLQRLPRIVGYGKAAELALTGR 180
           GCIGGG+DI TACDLRYCTAEAVFSV+EV +AITADLGTLQRLP IVG+GKA ELALTGR
Sbjct: 121 GCIGGGVDIATACDLRYCTAEAVFSVKEVNLAITADLGTLQRLPGIVGFGKAVELALTGR 180

Query: 181 DFSGLEAKELGLVSRTFDSISELQDGVLKIAQEIASRSPLAVVGTKAVLLKSRDLNVEQG 240
           +FSGLEAKELGLVS TF S  EL +GV+KIA+EIAS+SPLAVVGTKAVLLKSRDL VEQG
Sbjct: 181 EFSGLEAKELGLVSTTFASKMELDNGVIKIAEEIASKSPLAVVGTKAVLLKSRDLTVEQG 240

Query: 241 LDYVATWNSGTLLSDDLKEAISAQANKRQPKFSKL 276
           LDYVATWNSGTLLS DLKEAISA+A+KR P FSKL
Sbjct: 241 LDYVATWNSGTLLSADLKEAISARAHKRNPTFSKL 275

BLAST of PI0002897 vs. TAIR 10
Match: AT5G43280.1 (delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1 )

HSP 1 Score: 355.5 bits (911), Expect = 3.8e-98
Identity = 176/276 (63.77%), Postives = 221/276 (80.07%), Query Frame = 0

Query: 1   MEGYKCIKIERQNPNSGVFHIRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60
           ME YK ++I R+N +S VFH+ ++RPSH NAL+   F E  +A S LDQNPDV VIILSG
Sbjct: 3   MESYKTLEIIRKNTDSSVFHLIINRPSHLNALSLDFFIEFPKALSSLDQNPDVSVIILSG 62

Query: 61  SGKHFCAGIDLKSTAS-NFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIH 120
           +GKHFC+GIDL S +S + +     +RGR+ E++RR+IK MQ +ITAIE+CRKPVIA+IH
Sbjct: 63  AGKHFCSGIDLNSLSSISTQSSSGNDRGRSSEQLRRKIKSMQAAITAIEQCRKPVIAAIH 122

Query: 121 GGCIGGGIDIVTACDLRYCTAEAVFSVREVKMAITADLGTLQRLPRIVGYGKAAELALTG 180
           G CIGGG+D++TACD+RYC+ +A FS++EV +AI ADLGTLQRLP IVGY  A ELALT 
Sbjct: 123 GACIGGGVDLITACDIRYCSEDAFFSIKEVDLAIVADLGTLQRLPSIVGYANAMELALTA 182

Query: 181 RDFSGLEAKELGLVSRTFDSISELQDGVLKIAQEIASRSPLAVVGTKAVLLKSRDLNVEQ 240
           R FSG EAK+LGLVS+ F S SEL +GV  IA+ I  +SPLAV GTKAVLL+SR+++VEQ
Sbjct: 183 RRFSGSEAKDLGLVSKVFGSKSELDNGVTTIAEGIGGKSPLAVTGTKAVLLRSREVSVEQ 242

Query: 241 GLDYVATWNSGTLLSDDLKEAISAQANKRQPKFSKL 276
           GLDYVATWNS  L+SDDL EA+SAQ  KR+P+F+KL
Sbjct: 243 GLDYVATWNSAMLISDDLNEAVSAQMMKRKPRFAKL 278

BLAST of PI0002897 vs. TAIR 10
Match: AT5G43280.2 (delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1 )

HSP 1 Score: 268.9 bits (686), Expect = 4.6e-72
Identity = 132/214 (61.68%), Postives = 168/214 (78.50%), Query Frame = 0

Query: 1   MEGYKCIKIERQNPNSGVFHIRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60
           ME YK ++I R+N +S VFH+ ++RPSH NAL+   F E  +A S LDQNPDV VIILSG
Sbjct: 3   MESYKTLEIIRKNTDSSVFHLIINRPSHLNALSLDFFIEFPKALSSLDQNPDVSVIILSG 62

Query: 61  SGKHFCAGIDLKSTAS-NFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIH 120
           +GKHFC+GIDL S +S + +     +RGR+ E++RR+IK MQ +ITAIE+CRKPVIA+IH
Sbjct: 63  AGKHFCSGIDLNSLSSISTQSSSGNDRGRSSEQLRRKIKSMQAAITAIEQCRKPVIAAIH 122

Query: 121 GGCIGGGIDIVTACDLRYCTAEAVFSVREVKMAITADLGTLQRLPRIVGYGKAAELALTG 180
           G CIGGG+D++TACD+RYC+ +A FS++EV +AI ADLGTLQRLP IVGY  A ELALT 
Sbjct: 123 GACIGGGVDLITACDIRYCSEDAFFSIKEVDLAIVADLGTLQRLPSIVGYANAMELALTA 182

Query: 181 RDFSGLEAKELGLVSRTFDSISELQDGVLKIAQE 214
           R FSG EAK+LGLVS+ F S SEL +GV  IA++
Sbjct: 183 RRFSGSEAKDLGLVSKVFGSKSELDNGVTTIAED 216

BLAST of PI0002897 vs. TAIR 10
Match: AT4G16800.1 (ATP-dependent caseinolytic (Clp) protease/crotonase family protein )

HSP 1 Score: 127.1 bits (318), Expect = 2.2e-29
Identity = 82/269 (30.48%), Postives = 132/269 (49.07%), Query Frame = 0

Query: 7   IKIER-QNPNSGVFHIRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIIL-SGSGKH 66
           +K+ R    +SG+  + L RP   NA+  ++   L+ AF  + Q+    V+++ S     
Sbjct: 43  VKLNRLSGSDSGIIEVNLDRPVTKNAINKEMLKSLQNAFESIHQDNSARVVMIRSLVPGV 102

Query: 67  FCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHGGCIG 126
           FCAG DLK           E R  +   +   +  ++   + IE    P IA+I G  +G
Sbjct: 103 FCAGADLK-----------ERRTMSPSEVHTYVNSLRYMFSFIEALSIPTIAAIEGAALG 162

Query: 127 GGIDIVTACDLRYCTAEAVFSVREVKMAITADLGTLQRLPRIVGYGKAAELALTGRDFSG 186
           GG+++  ACDLR C   AVF + E  +AI    G  QRL R+VG   + EL  TGR    
Sbjct: 163 GGLEMALACDLRICGENAVFGLPETGLAIIPGAGGTQRLSRLVGRSVSKELIFTGRKIDA 222

Query: 187 LEAKELGLVSRTFDSISELQDGVLKIAQEIASRSPLAVVGTKAVLLKSRDLNVEQGLDYV 246
           +EA   GLV+    +  E  +  +++AQ+I  + PLA+   K  + +  + N+  GL+  
Sbjct: 223 IEAANKGLVNICV-TAGEAHEKAIEMAQQINEKGPLAIKMAKKAIDEGIETNMASGLEVE 282

Query: 247 ATWNSGTLLSDDLKEAISAQANKRQPKFS 274
                  L + D  E ++A A KR+P ++
Sbjct: 283 EMCYQKLLNTQDRLEGLAAFAEKRKPLYT 299

BLAST of PI0002897 vs. TAIR 10
Match: AT4G16210.1 (enoyl-CoA hydratase/isomerase A )

HSP 1 Score: 104.0 bits (258), Expect = 2.0e-22
Identity = 61/207 (29.47%), Postives = 103/207 (49.76%), Query Frame = 0

Query: 9   IERQNPNSGVFHIRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSGSGKHFCAG 68
           I+ +  + G+  I ++RP   N+LT  +  +L +AF  +D +  V V+I +GSG+ FC+G
Sbjct: 10  IQVKKESGGIAVITINRPKSLNSLTRAMMVDLAKAFKDMDSDESVQVVIFTGSGRSFCSG 69

Query: 69  IDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHGGCIGGGID 128
           +DL +  S F+  + +                 + +  +E  RKP+I +I+G  I  G +
Sbjct: 70  VDLTAAESVFKGDVKDPE--------------TDPVVQMERLRKPIIGAINGFAITAGFE 129

Query: 129 IVTACDLRYCTAEAVFSVREVKMAITADLGTLQRLPRIVGYGKAAELALTGRDFSGLEAK 188
           +  ACD+   +  A F     +  I    G  Q+L RI+G  KA E++LT    +   A 
Sbjct: 130 LALACDILVASRGAKFMDTHARFGIFPSWGLSQKLSRIIGANKAREVSLTSMPLTADVAG 189

Query: 189 ELGLVSRTFDSISELQDGVLKIAQEIA 216
           +LG V+   +     +   LK A+EIA
Sbjct: 190 KLGFVNHVVE-----EGEALKKAREIA 197

BLAST of PI0002897 vs. TAIR 10
Match: AT4G29010.1 (Enoyl-CoA hydratase/isomerase family )

HSP 1 Score: 89.0 bits (219), Expect = 6.6e-18
Identity = 57/211 (27.01%), Postives = 101/211 (47.87%), Query Frame = 0

Query: 5   KCIKIERQNPNSGVFHIRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSGSGKH 64
           K I +  +  N GV  I +  P   N+L + + + L++ F   +Q  DV  I+L G+   
Sbjct: 3   KKIGVTMEVGNDGVAVITISNPP-VNSLASPIISGLKEKFRDANQRNDVKAIVLIGNNGR 62

Query: 65  FCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHGGCIG 124
           F  G D+         ++ ++  + G+        ++     +E+ RKPV+A++ G  +G
Sbjct: 63  FSGGFDI---------NVFQQVHKTGDLSLMPEVSVELVCNLMEDSRKPVVAAVEGLALG 122

Query: 125 GGIDIVTACDLRYCTAEAVFSVREVKMAITADLGTLQRLPRIVGYGKAAELALTGRDFSG 184
           GG+++  AC  R    +A   + E+ + +    G  QRLPR+VG  KA ++ L  +  S 
Sbjct: 123 GGLELAMACHARVAAPKAQLGLPELTLGVIPGFGGTQRLPRLVGLAKATDMILLSKSISS 182

Query: 185 LEAKELGLVSRTF---DSISELQDGVLKIAQ 213
            E  +LGL+       D +S  +   L IA+
Sbjct: 183 EEGHKLGLIDALVPPGDVLSTSRKWALDIAE 203

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FHR85.3e-9763.77Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal OS=Arabidopsis thaliana... [more]
O354593.8e-5541.98Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial OS=Mus musculus OX=10... [more]
Q5RFG01.1e-5442.75Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial OS=Pongo abelii OX=96... [more]
Q130111.5e-5443.13Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial OS=Homo sapiens OX=96... [more]
Q626513.3e-5441.60Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial OS=Rattus norvegicus ... [more]
Match NameE-valueIdentityDescription
A0A5D3D1B61.5e-14797.09Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal OS=Cucumis melo var. ma... [more]
A0A1S3BXS51.5e-14797.09delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal OS=Cucumis melo OX=3656... [more]
A0A5A7TMZ41.3e-14696.73Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal OS=Cucumis melo var. ma... [more]
A0A0A0KXB81.3e-14696.36Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G011790 PE=3 SV=1[more]
A0A6J1GW607.4e-12684.36delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal OS=Cucurbita moschata O... [more]
Match NameE-valueIdentityDescription
XP_008454026.13.2e-14797.09PREDICTED: delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal [Cucumis mel... [more]
XP_004152122.12.7e-14696.36delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal [Cucumis sativus] >KGN5... [more]
KAA0044560.12.7e-14696.73delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal [Cucumis melo var. maku... [more]
XP_038879593.11.6e-13892.00delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal isoform X1 [Benincasa h... [more]
XP_023526792.11.2e-12584.73delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal [Cucurbita pepo subsp. ... [more]
Match NameE-valueIdentityDescription
AT5G43280.13.8e-9863.77delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1 [more]
AT5G43280.24.6e-7261.68delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1 [more]
AT4G16800.12.2e-2930.48ATP-dependent caseinolytic (Clp) protease/crotonase family protein [more]
AT4G16210.12.0e-2229.47enoyl-CoA hydratase/isomerase A [more]
AT4G29010.16.6e-1827.01Enoyl-CoA hydratase/isomerase family [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014748Enoyl-CoA hydratase, C-terminalGENE3D1.10.12.10coord: 218..275
e-value: 9.8E-25
score: 88.5
IPR001753Enoyl-CoA hydratase/isomerasePFAMPF00378ECH_1coord: 15..273
e-value: 2.8E-52
score: 177.6
NoneNo IPR availableGENE3D3.90.226.10coord: 1..217
e-value: 2.7E-65
score: 221.8
NoneNo IPR availableCDDcd06558crotonase-likecoord: 7..214
e-value: 3.13867E-58
score: 182.375
IPR045002Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase Ech1-likePANTHERPTHR43149ENOYL-COA HYDRATASEcoord: 2..275
IPR018376Enoyl-CoA hydratase/isomerase, conserved sitePROSITEPS00166ENOYL_COA_HYDRATASEcoord: 115..135
IPR029045ClpP/crotonase-like domain superfamilySUPERFAMILY52096ClpP/crotonasecoord: 4..273

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0002897.1PI0002897.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0044281 small molecule metabolic process
cellular_component GO:0005777 peroxisome
molecular_function GO:0051750 delta3,5-delta2,4-dienoyl-CoA isomerase activity
molecular_function GO:0003824 catalytic activity