PI0002717 (gene) Melon (PI 482460) v1

Overview
NamePI0002717
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptionmechanosensitive ion channel protein 2, chloroplastic-like
Locationchr06: 22851900 .. 22861342 (+)
RNA-Seq ExpressionPI0002717
SyntenyPI0002717
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGAAAAGTGGGTCCGAATCTGTTTCTTTTTCATTTCTCTCCTTATGCATGAACGTCCAAAGAAACAGAGAAAACAGAGACCCTGAGCTCGATTGGAAGCTGTAGAACTTTTCCCATCAACGAAATTTGATTCTCGTTTGAGTTGGTAACATCCTCATTTAATACTAATTTTCCTGTTATGCCCAAACTAACTTTGTCGTCCCTCTGTTCTTCTTTCTTTGTACCTTCACCATTTTTTTTTTTCTTATTCCACCACCTTCCTCATCGTGATTATCGGTTACCCAATTTCAGATCAATAGCAGGCTATTGTTTGTATGTATACAATCAGCTCTCATCCTTGAAATTATGTTTCCTTGCTTCTACCGTTTGACTTTTTCTGAAGTTACCGTTTGTAGAATTTCGTTTCACGTTTGAGTCTTCATCTGGAAGTTAGGGTTTGGAGAATAATGTTATGGCTTTCGGTTGAGTTATTGCCTCTTGTTTATTATTTCACTGCTCTTTTTATTATCATGTTTATATCTTAAATCCAGGATAAATTTAAGTTTCGTACTCTGATTGGGGCTTTGTAGGGTCAATAATTATTTTTCTTCATTGTTATTAATTTTTTGGTGTGACTTGGGACCATGTAAGACTTACCCAGCAAAGAACCTTACTTTCTTAGAAAGAAAAAACTAAGGTCTTGAGGGGAGATAGGAAACAAAGAATGAGGAAAAATGATCGACTTGACAATCCTTCAGACTGATTAGGCACCGAAACTCGGACATCCCTTCTACTATAAAACAAACTCTGATTGGAGGTGGTGGAAAGAAGTTCCAGCACCTAACTATCTCCAAAGAATGATATGATTACTCGGAGGACGAGAAAACAAATGCCACCAAATTTAACCTCTTATACTTTGATGAGTTGTCAACTTGGCGGGGCCATGTAATGTAAAATGTTCCTCTATTTTCTTGTTTTACTGAAAATATTTAAAGTCAGCTTCCTCTTAATCATTCGTTATATGTAACAAAATAATTTGATAGCATACAAAGTCTGTGTGTGCATGTCTGCGTGGATAATGTTAGATGCCAATGGGCATTGCCCGATTAATGAGCAAGTTAGATAACGAAGGTGGAAGAAATAATTTAGATTCTTGACTATCAATTGTTAGTAGTAGTCTCAGTACCATTTACATTGATTTCATGATAAATGTGTCAATAAAGTACGATTTGGTGCAGTTTTCAGCTGTCATTGTGCTGCAAGGAGTCCCATTGTTCAAGTTCCATCTTGCCAGCATTTATTTAGTTTTATCAATGGTTCTTGTTGGGTCTTTGCAACTGTCCTATCATCTAGGACCGTGGAGAAATTATTTGCATGAAAAAAATCTGAAGGTACAGAAACTTTCTCCCCTGCCTCCAGACAATATGTAATTCTCAGGTAAATATATGTGTTTCAGTGTCTCTGTTTGTTCTTATACAAAACTTAATTGTTCCTTACAGTTTACGTCCCAGAGCTATGGCATCCGGCTTCTGAATGTAGCCAGTTCATCATCTCTTTTGGTAAGTTCTTTAAACACTTTGGTTAATTTTAAAATCAGAACATAAAAACTTATTGTTCTTGGAGATCATCAAGAATGTCTCCCTCATATCAGCATGGGTTTGAATGCTTTCAATGTGTATTTGGGTGTGTATATTTGGTGTGTTGATTGCATGTGGAAATTCTTGCTTTTAAGAGTATATATTTGAGAATATTTCTTATTGCAGTTTCAGGACACTTGGAGCACTCATTTATTTAGCATGAAATACCCACCTAATTACACTGTGCCTTCTAGATACAATGTATTCAGGTGTCGGTCGTCATTGTTGACAAACCAACCACTGGATCCTCCTGGGATGAAGGCTGCTATAGTGACATTGACGAGGTTAGTTTCTGGGTTCGTGTATCAATATTTTGTGCCTAATTTTTTTTTCGAATGTTTGACTTATATAAGATTGTTCTCACTGTCATAGGTAGAAATAAACATCTTATTTTTATCCCTGTTTACACACTCATCCACTATTAACATCAAAGACATTGTGATAAAAAATCACCCCATCTGAATGTAGTTTAGATAAGTTTATTACTTTGTCCCCAAATATTGGTTCCCATTTCATTTGGCCTAGTTATGAAACATTTATGTTGGTTTTTGGTTATGATATATAGGAAAATTTCAGTAGAACTTTAGATGGATACATCAGTGCTTACATGGGTGATGTTGGATAATATATTGCAATTTACATCTAATCGTTTGAGACAAGTTGAACTAAGGACAATATTAGAGCTTTGATAAAACAATAAAATTAGGGATTTTGGATCAGCAGCATTTTCTCAAAATACTTGATTGAATCTCTACCATCTGAGAAAATATTCTTTACATTCTCCTGGAAAAGACAACTATCCATCATCATCATCCTTCCTATGAAGAATGCCTACACTATCTCCAGAAAATTATTATTCCTTCAGCCTTCAGGCTTCAAATGACTGTATTTTATTCATATTCGTGACTTTTTGGTTGTTTTTCTCACATCTCTATTCTCACACTAAAAGTATTTTAATTGTGTATCTAGTGGTTTTTATGGGGCTAGCAGTTTTATGCTGGTGTATGAGAGAGAAACTGTAAAAGTATTTTAATTGTGTATCTAGTGGTTTTTATGGGCATGCAAATTTTTGAATCTACACTTATGGTAGGATCAATTGGAGCATTATTTAGGACCCTGAGCTAGGAACTATATTTTTCCTTCTAGAGGCAAGTTTTAATACACAGATAGCCAATTAACCACGCTAATAATGTTGCAAAATAAGCAATTAGCAAGTCATCTTATCTGTGTTGAAAACATTGCAGCATAGTTAACCCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGCGGACGGACTTACCTGGTGAAAGTAAAAGTTGTGTGCTTATATCATGAAAACTCAAGTAGGAAGTGGCCACTAATTTTGGGAGAAAGTTAACAGTTTATCCTCATTTTTTCTACTGATGAAATTAAGCTTAAGGACTTCATTTTTTCATGGTCTTATCATGATTGTAATGGTAGTAAAGACTTTAGAAACCGAGATACGTGGAAACCCGAGTACCAGGAGAAAAACCACGATTGTTACTTTCTTATTAGTTTTTGATGATTACAGAAAAGATACAAGAGGCTGAGAAGCTTAAAATAAAAAAGGAAAGGAATTTAGGTCAAATCCTTCCTTGGGCCAAGCCCAATAATTCTAACAACAGGTACATTCAGTATACCTGTCCTAGGGATGCTCATTGACGAATTATTTCTTTTTGTTTTGTTTTTTTTACTAATATACGCTAAATATACAATCTTTTTGCCTAAATGCATGTGCCATTTTCAATTTGTTGCTTCAATCTTCTGAAAAAGTTTGATACTTTGCATATGGTTTAAATCATATTTTGGTCCCTAAAATCTGTATCTTATTCTAATTTGGTCCCTTAACTTTTAAAAATGTATATTTTAATCCCTGAATTTTTTAAAAGAGCATATTTTGGTCCCTACCATTAGGATTCCGTTAACTCTTTAACAAAATGATGATGTGGCTTTTATATGTGGATGCCTAGGCTACCAAATAAGATGATCACTCTTAAAAATTCAGGGTTTTCATTTTTTATTAGAGGTCAGATGGTAAATTGATGTTTTATTGATGAACTCAAAAAGTCACTTTGACTTCAAGATTTTGATTGCTATCATTTTGGTTCATTGACATTCTACTCACTTTCTCAACTCATGCCTCAAGTTTTGGATATTTGGTGATCCTTTTTTTTTGGAGGAGGTGGGGGTGGATTCATTGTGAGAATGTTTATTTGCAATTTTGTAGAGTTTATTTTATACATAAGTTGTACTTATTTTAATTTTTTAGTTCATTTTATTTTTACCTTGCCATAGATGTTACCACCAATATGTCAATTCTATTTTATTTTTTTATTCTACTCAATGTTCTTTTGATATGTTTAAGATTCCAATTTGGAAAAAAAAAATAGTATAGGGTGGGGGATGAGAGATTTGAGTTTTGGAGAAAAAAAGAACAAAATTTAGTTTTGGGTTTATTTGATAGATGTGTTAATTATGTTTCTGCCTTGGCATAAAAGATTTCTAGAATAAATTAGCAAGATTTCTGGGTTATTTATCAATTTAAATAATATGATAGATCTAGAGCTTAATCATCCAAATATAGAAGTCACATCATCATTCCGTTAAAGAGTTAACGGAATCTTAATGACAGAGACCAAAGTAAGCTTTTTTATAAAGTTCGGGGACTAAAACAGACGTTTTTAAAAGTTTAGTGACCAAAATATAACAAGATACAAAGTTTAGGGACCAAAATATGTTTTAAACCCTTTACATATTAAGTTGTTTCACTAAGTTATTTCTTCTATTGTAGTACACGCTAAAAAAATGGCTCGATTAAATTATTATGGATTGTGTGTGCTATGTGAAATGGCTAACTTTTTTTCTTTTTGCATATGAGGGAGTTCAATCAATAAAATTGAAGCATAGGATGGTTCCATTTCAACTCGGTTAATGAAATATATTATGATGTTCGAATGTGACTTCTCATTTATAGGTTTTAAGGTTTTGTGATACGGTAAAGACTGGGCTGAAGAACCTTATCGTATGGAGGATGAAACTAAACTTTTTGGAAGGCTAGGGACAAAATTGAAAAGTGACAAGCATATAAAGCCATCAAGATGTATATTTAGTTTAATCTTAATTGAGATGTTCTCTGGCCTTTGTCTTATGGACTCTTGAAAATAATATGCATTCATCTTTTGAGTAATTAATAATATTTTCATTGTGGCAAAATATCCATGTGTGATGTGTCTATTAAATCATTCATATAGTTTCTACACGAGCTTGAAGATTGAATTTGATTGTATTTGAGCTCTGCTGGTTTTTCACATTTGATCATATAACGCAGGTTTTGTAATGTATTAGGTGGTTGTCCTCCTCCAGTGATTAAGTTAGTTCCCGCAGTTTGTATTATAATATTTGCTGTATGGGGTCTTGGACCATTTTTGCGTTATACGAGAAGCCTATTCCATGTATGTTGACTTAACTTCTGTTTCTTTTTGTCAGATTGCCATTGTTTGTTCTTTCTTTTCTCTTTAATTGTTCTACTGTTTTCATATTGTTCTATAATTTTTCTGCAGAATGATAGCAACTGGAAGAAAAGTCGGACATATAATGTCATGACCTTGTATCTTCAACCATTATTGCTATGGACTGGAGCCACACTTATTTGCAGGTTCTTGAGATACAGTTTTTTTATATGCTGACTTGAACTGATCTTTTCAGTTGCAGTTTCCAAGATATTTTTCTCACTTATTTTGTTTGATATTCTTGATCTTGCTTTTGCAGAGCTTTAGATCCAGTAGTTTTACGTACAGAGTCCAGCCAAGTTGTGAAGCAACGAGTGTTGAATTTTGTTAGGTCGTTGTCCACTGTGCTGGCCTCAGCATATTGTTTATCTAGGTGAGCATATGAGAACTCGTCGTTTGAAATGCTCAATGAGTTTACCCGTATAATGAAGAAATCTACCGTCACTATCAACATGATAATTTAACTTGCATCGATATGTTACCCTTACACAAGGCTTTTTGCTTATGAATGTTAAAATATTTTAAAATTCAATTTATTAACCCTTGCTCTCCTTGGTCCCCTTTTTTTCTTTTTGCTCCTGCCTGTGTAGCATGATTCAACAAGCACAGAAATTCTTCTCAGAGAGTACTGAATCCAGTGACGCGAGAAATGTATGTACCATGACATCACATTTTTACTCTAAGCTTGATAGATATCATGTAGTTAGGTTTCTGATTGTTGGTGAGCATTTTATTGTGACAAATCGGCTGTCTTTTGTTATAAGCTCTGTGTTGTATGGAGTATGGTATGTGAGATTATACTTGAAGGCACCTTTTGAATATCGGTTTCATGTTCTTGTATGATGCAATTTAGCACAACCAGTGGAACCTGTTGCTTATACATGGCTATATTGCTTACTTTCACTTTGAAGACAATTTGAGTGGCATCCAATATCTTTTTTATGCCAGTATCAGAAAGAAATTACTCAAACAGCTTTTCATTTATGAAATTACTAACTTCAAGTACACATAATCATATGGAGCTATTTGTTTTCAGTTAAATTTTGCATTGATATCATATGATATGGGTTTCTGTATCCTTGAGATGTGGTTCTCACTGAAACTTTTGTTTAATGGTTTATTTAAAACCGTGGAAGTGTGATATAAATTTGTCTATGGGCAGATGGGCTTTCAATTTGCTTGGAAAGCTGTATACTCTGCAGTATGGGTTGCTGCTGTCTCATTGTTCATGGAGCTATTGGGCTTTTCAACCCAAAAATGGCTTACAGCTGGAGGTCTTGGGACTGTATTGTTGACATTAGCTGGCCGTGAGGTACTTTTGATTGGTGGTATGGTTTGTGTTTGCTGTTTCTTTGGAGTTTATTTTAAACTAATTTTAGATTTTTCTTTCTGAACCTAGATTTTCACCAACTTTCTCTCAAGTGTGATGATTCATGCAACTCGTCCTTTTGTTGTTAATGAGTGGATCCAAACGAAGATCGAGGGCTACGAAGTTTCTGGTACTGTTGAGGTTTGTATGTTCCTGTTGAACATGTACCATAAAATGTTGATGTCAAGAAAGTAATTCGCCAAAATCTAGTATTTCAATGTACACCAATGTCTTAATTTAATGTATCGTTAGAAACAGTAACATGTCGTATATGAACAAACACATGAGACATTTATTTCCATTTCAATCAACTGCAGCACGTAGGGTGGTGGTCGCCAACAATCATTAGAGGTGAAGATCGTGAAGCAGTTCACATACCAAACCATCAATTTACCATGAATGTCGTTAGAAATCTAAGTCAAAAAACTCACTGGCGGATTAAAACCCACATTGCCATCAGCCATTTGGACGTCAATAAAATCAATGTGAGAATATTTTGTTTTGATCTTCTTGGTATGAATGTATGTATTCATGCTATATAGCACATATTTTATGCTGATTGTATTCTTATCTCTCCACAGAACATTGTCGCAGACATGCGGAAAGTCTTGGCAAAGAACCCTCAAGTTGAACAACAGAGATTGCATAGAAGAGTATTTTTGGAAAACGTGGATCCTGAAAATCAGGCACTTTTGGTCAGTATTAGACATTTTGGCTTAAATATTATGTTTCTCTTTGAAGTTTTCAATTTTGTCCTCAAACGTTTGAATAATTTAAATAAACTCCTCAACTTAAATGTGGAAAGCACATCTAAATGGAGTGATTTTGGTAATGGAAAAATGATTTTGACAAACATGACAGTGAAATGTTGCAAATTTAGCATATTCATTTTATGAATTTTGCCTACTTGAATAAGAACCAAGTGAATAGTTTGTAATGTTGAGTTGGTTGATGCATCTTTAAGTGTGCTCGCTTTACCTGGTATGGTGGAATGACAATACTTGGAAGGACAGTTTTAGAATTAACGTTCTCTTGGATTTGGCCCCATTATAGATCTAAGATCTTTACTTATCCTGAATTTATTAGTTTGGGGAATTAATCAATTGACTTAATCTTCAGAACATTATAGTTTTAGGATCTGTGATTGTTGCCAAGACAAACTGATAGATTATGCACGCTTCGTCATAAATCCTTGTTCGTTTTATATCAAGTTTTAGGGGGCTCTATCTCATGTTTCCTAATTGATTTATGTCCTATTTTTGCTAGATTTTGATATCTTGTTTTGTCAAGACTTCACATTTTGAAGAATACCTATGTGTAAAGGTAAATTGATCTTTTCTGCTTTATTATTTTTATTATTTTCACTCTTTTTTTAAAAAAAATTCTTTATACTTTTCGTTCTCTCAACTTTCTACTGGCAATTTCAGAAATTCCATTGATCCATTTTGTTGGTCTGGAGATTGTCTGAATACATTTGACTTCTGAGCCTTTCTTTTCTTTGAATAACTTGTTATCTGATGAATCAGGAAGCTATAATTTTGGATCTTCTTAGAGTCATTAGACACCACCGGGCTCGGCTTGCAACGCCAATCCGCACAATGCAGAAGATGCATAGTGATTCAGACTTGGAAAGTGTTCCATTTTCTGATTCAATATTTGGCCAGCGTGGTGCAACTTTGAATCGCCGCATGCTAATGATTGAGCCTCCTTACAAAGTTTATGGAGAAGATAGAAAACAAAGCCATAGCACTAACAGGACATCACGCACAACTGGAGAACAAAATGGTAAGCCCATTGCACGATCATCGGGTGACAGCAAAGCAGCTAAAGAGACAATGTCATCTGATAGAAAGACAGAAGTCAAGACTGGAGGAGACGCTGATACAAAAAAGCATTTCAAAGTTTCAATGTCAGCCTCTGAAGACAAATCAAGCAATGAATTGAAACATAAACCATCATCAAAGTCTGCTGCAAGTACCTCTGGTATGCCAACTTTTGATGCCAAACCAACTAAATCAGATGCTGATAATTCATTGGAAGATTCCAGTCCCAAGCAATCCGAAGACAGCATGGGAAGTAACACGCAGAACTTCAAGCCTTCCCTTCCTGCTGTTTCTTCTCCGGAAGATGTTAAGAAACCCGGAGGAACTACTTCAGCAGCAGCTTCACAGCCAAGAATAGAAGGTGAGCAGACAACAGTTTCAAATACAACTGTTTCAAATCCATCAGCAAAGAAGCCTGGGGTAGAAGAGAATATAATCCTTGGTGTTGCTTTGGATGGCTCTAAGAGAACACTCCCTATTGAGGACAATGTACCTACTACTGCATCGGGAGCAAAAGATTTGGCTGCATTCAATGGAGCAACAATGGCAGATAAGAATACGAAACGTCAGTCTCCTTCATCTCCTACCACATCTTCTAGTGAATAGTGAGACTAGTTTGATGCAGGGGATCTAGATAAGTAAACAACTTTTTTTGACAGAGAAGTTTGCATTGAGGCCCTTTGAAGCTGCGTTTTTTCAGTGCTGCAACAAAGTATTCTCACCTGACTACAGTTTGTTTGCAACGCAATTAGTGTAGGGCATGAATGTACATTGGTGTCATTTTGACTCTTGTTTCACTGAATTCTGAAACAACAAACTGATTACTTTGGGGAGAAGCGGCAGTTGATGACAACACTTCGTTTTAAAGAAGTTTCAGTTGCAGTTTTTTGATAGAGCTTACGCTTTGTACATAAAACCTGTATCTTGTGAACTATAAATTACGTTTACAAACAAAGGATATGCTGGATTGAATCAAATGCCTTTTGTTGAAGGATCTGAATTTTGTTCAAGAGAATGCCAGGGATTGCGCATATACTGAACAAAATGGCTCAGTTTGTAGTGATTAACGTGCTCCGAGTCTAACAAATTTTGCGTTTTTTT

mRNA sequence

TGAAAAGTGGGTCCGAATCTGTTTCTTTTTCATTTCTCTCCTTATGCATGAACGTCCAAAGAAACAGAGAAAACAGAGACCCTGAGCTCGATTGGAAGCTGTAGAACTTTTCCCATCAACGAAATTTGATTCTCGTTTGAGTTGTTTTCAGCTGTCATTGTGCTGCAAGGAGTCCCATTGTTCAAGTTCCATCTTGCCAGCATTTATTTAGTTTTATCAATGGTTCTTGTTGGGTCTTTGCAACTGTCCTATCATCTAGGACCGTGGAGAAATTATTTGCATGAAAAAAATCTGAAGTTTACGTCCCAGAGCTATGGCATCCGGCTTCTGAATGTAGCCAGTTCATCATCTCTTTTGTTTCAGGACACTTGGAGCACTCATTTATTTAGCATGAAATACCCACCTAATTACACTGTGCCTTCTAGATACAATGTATTCAGGTGTCGGTCGTCATTGTTGACAAACCAACCACTGGATCCTCCTGGGATGAAGGCTGCTATAGTGACATTGACGAGGTTTTGTAATGTATTAGGTGGTTGTCCTCCTCCAGTGATTAAGTTAGTTCCCGCAGTTTGTATTATAATATTTGCTGTATGGGGTCTTGGACCATTTTTGCGTTATACGAGAAGCCTATTCCATAATGATAGCAACTGGAAGAAAAGTCGGACATATAATGTCATGACCTTGTATCTTCAACCATTATTGCTATGGACTGGAGCCACACTTATTTGCAGAGCTTTAGATCCAGTAGTTTTACGTACAGAGTCCAGCCAAGTTGTGAAGCAACGAGTGTTGAATTTTGTTAGGTCGTTGTCCACTGTGCTGGCCTCAGCATATTGTTTATCTAGCATGATTCAACAAGCACAGAAATTCTTCTCAGAGAGTACTGAATCCAGTGACGCGAGAAATATGGGCTTTCAATTTGCTTGGAAAGCTGTATACTCTGCAGTATGGGTTGCTGCTGTCTCATTGTTCATGGAGCTATTGGGCTTTTCAACCCAAAAATGGCTTACAGCTGGAGGTCTTGGGACTGTATTGTTGACATTAGCTGGCCGTGAGATTTTCACCAACTTTCTCTCAAGTGTGATGATTCATGCAACTCGTCCTTTTGTTGTTAATGAGTGGATCCAAACGAAGATCGAGGGCTACGAAGTTTCTGGTACTGTTGAGCACGTAGGGTGGTGGTCGCCAACAATCATTAGAGGTGAAGATCGTGAAGCAGTTCACATACCAAACCATCAATTTACCATGAATGTCGTTAGAAATCTAAGTCAAAAAACTCACTGGCGGATTAAAACCCACATTGCCATCAGCCATTTGGACGTCAATAAAATCAATAACATTGTCGCAGACATGCGGAAAGTCTTGGCAAAGAACCCTCAAGTTGAACAACAGAGATTGCATAGAAGAGTATTTTTGGAAAACGTGGATCCTGAAAATCAGGCACTTTTGATTTTGATATCTTGTTTTGTCAAGACTTCACATTTTGAAGAATACCTATGTGTAAAGGAAGCTATAATTTTGGATCTTCTTAGAGTCATTAGACACCACCGGGCTCGGCTTGCAACGCCAATCCGCACAATGCAGAAGATGCATAGTGATTCAGACTTGGAAAGTGTTCCATTTTCTGATTCAATATTTGGCCAGCGTGGTGCAACTTTGAATCGCCGCATGCTAATGATTGAGCCTCCTTACAAAGTTTATGGAGAAGATAGAAAACAAAGCCATAGCACTAACAGGACATCACGCACAACTGGAGAACAAAATGGTAAGCCCATTGCACGATCATCGGGTGACAGCAAAGCAGCTAAAGAGACAATGTCATCTGATAGAAAGACAGAAGTCAAGACTGGAGGAGACGCTGATACAAAAAAGCATTTCAAAGTTTCAATGTCAGCCTCTGAAGACAAATCAAGCAATGAATTGAAACATAAACCATCATCAAAGTCTGCTGCAAGTACCTCTGGTATGCCAACTTTTGATGCCAAACCAACTAAATCAGATGCTGATAATTCATTGGAAGATTCCAGTCCCAAGCAATCCGAAGACAGCATGGGAAGTAACACGCAGAACTTCAAGCCTTCCCTTCCTGCTGTTTCTTCTCCGGAAGATGTTAAGAAACCCGGAGGAACTACTTCAGCAGCAGCTTCACAGCCAAGAATAGAAGGTGAGCAGACAACAGTTTCAAATACAACTGTTTCAAATCCATCAGCAAAGAAGCCTGGGGTAGAAGAGAATATAATCCTTGGTGTTGCTTTGGATGGCTCTAAGAGAACACTCCCTATTGAGGACAATGTACCTACTACTGCATCGGGAGCAAAAGATTTGGCTGCATTCAATGGAGCAACAATGGCAGATAAGAATACGAAACGTCAGTCTCCTTCATCTCCTACCACATCTTCTAGTGAATAGTGAGACTAGTTTGATGCAGGGGATCTAGATAAGTAAACAACTTTTTTTGACAGAGAAGTTTGCATTGAGGCCCTTTGAAGCTGCGTTTTTTCAGTGCTGCAACAAAGTATTCTCACCTGACTACAGTTTGTTTGCAACGCAATTAGTGTAGGGCATGAATGTACATTGGTGTCATTTTGACTCTTGTTTCACTGAATTCTGAAACAACAAACTGATTACTTTGGGGAGAAGCGGCAGTTGATGACAACACTTCGTTTTAAAGAAGTTTCAGTTGCAGTTTTTTGATAGAGCTTACGCTTTGTACATAAAACCTGTATCTTGTGAACTATAAATTACGTTTACAAACAAAGGATATGCTGGATTGAATCAAATGCCTTTTGTTGAAGGATCTGAATTTTGTTCAAGAGAATGCCAGGGATTGCGCATATACTGAACAAAATGGCTCAGTTTGTAGTGATTAACGTGCTCCGAGTCTAACAAATTTTGCGTTTTTTT

Coding sequence (CDS)

ATGGTTCTTGTTGGGTCTTTGCAACTGTCCTATCATCTAGGACCGTGGAGAAATTATTTGCATGAAAAAAATCTGAAGTTTACGTCCCAGAGCTATGGCATCCGGCTTCTGAATGTAGCCAGTTCATCATCTCTTTTGTTTCAGGACACTTGGAGCACTCATTTATTTAGCATGAAATACCCACCTAATTACACTGTGCCTTCTAGATACAATGTATTCAGGTGTCGGTCGTCATTGTTGACAAACCAACCACTGGATCCTCCTGGGATGAAGGCTGCTATAGTGACATTGACGAGGTTTTGTAATGTATTAGGTGGTTGTCCTCCTCCAGTGATTAAGTTAGTTCCCGCAGTTTGTATTATAATATTTGCTGTATGGGGTCTTGGACCATTTTTGCGTTATACGAGAAGCCTATTCCATAATGATAGCAACTGGAAGAAAAGTCGGACATATAATGTCATGACCTTGTATCTTCAACCATTATTGCTATGGACTGGAGCCACACTTATTTGCAGAGCTTTAGATCCAGTAGTTTTACGTACAGAGTCCAGCCAAGTTGTGAAGCAACGAGTGTTGAATTTTGTTAGGTCGTTGTCCACTGTGCTGGCCTCAGCATATTGTTTATCTAGCATGATTCAACAAGCACAGAAATTCTTCTCAGAGAGTACTGAATCCAGTGACGCGAGAAATATGGGCTTTCAATTTGCTTGGAAAGCTGTATACTCTGCAGTATGGGTTGCTGCTGTCTCATTGTTCATGGAGCTATTGGGCTTTTCAACCCAAAAATGGCTTACAGCTGGAGGTCTTGGGACTGTATTGTTGACATTAGCTGGCCGTGAGATTTTCACCAACTTTCTCTCAAGTGTGATGATTCATGCAACTCGTCCTTTTGTTGTTAATGAGTGGATCCAAACGAAGATCGAGGGCTACGAAGTTTCTGGTACTGTTGAGCACGTAGGGTGGTGGTCGCCAACAATCATTAGAGGTGAAGATCGTGAAGCAGTTCACATACCAAACCATCAATTTACCATGAATGTCGTTAGAAATCTAAGTCAAAAAACTCACTGGCGGATTAAAACCCACATTGCCATCAGCCATTTGGACGTCAATAAAATCAATAACATTGTCGCAGACATGCGGAAAGTCTTGGCAAAGAACCCTCAAGTTGAACAACAGAGATTGCATAGAAGAGTATTTTTGGAAAACGTGGATCCTGAAAATCAGGCACTTTTGATTTTGATATCTTGTTTTGTCAAGACTTCACATTTTGAAGAATACCTATGTGTAAAGGAAGCTATAATTTTGGATCTTCTTAGAGTCATTAGACACCACCGGGCTCGGCTTGCAACGCCAATCCGCACAATGCAGAAGATGCATAGTGATTCAGACTTGGAAAGTGTTCCATTTTCTGATTCAATATTTGGCCAGCGTGGTGCAACTTTGAATCGCCGCATGCTAATGATTGAGCCTCCTTACAAAGTTTATGGAGAAGATAGAAAACAAAGCCATAGCACTAACAGGACATCACGCACAACTGGAGAACAAAATGGTAAGCCCATTGCACGATCATCGGGTGACAGCAAAGCAGCTAAAGAGACAATGTCATCTGATAGAAAGACAGAAGTCAAGACTGGAGGAGACGCTGATACAAAAAAGCATTTCAAAGTTTCAATGTCAGCCTCTGAAGACAAATCAAGCAATGAATTGAAACATAAACCATCATCAAAGTCTGCTGCAAGTACCTCTGGTATGCCAACTTTTGATGCCAAACCAACTAAATCAGATGCTGATAATTCATTGGAAGATTCCAGTCCCAAGCAATCCGAAGACAGCATGGGAAGTAACACGCAGAACTTCAAGCCTTCCCTTCCTGCTGTTTCTTCTCCGGAAGATGTTAAGAAACCCGGAGGAACTACTTCAGCAGCAGCTTCACAGCCAAGAATAGAAGGTGAGCAGACAACAGTTTCAAATACAACTGTTTCAAATCCATCAGCAAAGAAGCCTGGGGTAGAAGAGAATATAATCCTTGGTGTTGCTTTGGATGGCTCTAAGAGAACACTCCCTATTGAGGACAATGTACCTACTACTGCATCGGGAGCAAAAGATTTGGCTGCATTCAATGGAGCAACAATGGCAGATAAGAATACGAAACGTCAGTCTCCTTCATCTCCTACCACATCTTCTAGTGAATAG

Protein sequence

MVLVGSLQLSYHLGPWRNYLHEKNLKFTSQSYGIRLLNVASSSSLLFQDTWSTHLFSMKYPPNYTVPSRYNVFRCRSSLLTNQPLDPPGMKAAIVTLTRFCNVLGGCPPPVIKLVPAVCIIIFAVWGLGPFLRYTRSLFHNDSNWKKSRTYNVMTLYLQPLLLWTGATLICRALDPVVLRTESSQVVKQRVLNFVRSLSTVLASAYCLSSMIQQAQKFFSESTESSDARNMGFQFAWKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVVNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTMNVVRNLSQKTHWRIKTHIAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVDPENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGQRGATLNRRMLMIEPPYKVYGEDRKQSHSTNRTSRTTGEQNGKPIARSSGDSKAAKETMSSDRKTEVKTGGDADTKKHFKVSMSASEDKSSNELKHKPSSKSAASTSGMPTFDAKPTKSDADNSLEDSSPKQSEDSMGSNTQNFKPSLPAVSSPEDVKKPGGTTSAAASQPRIEGEQTTVSNTTVSNPSAKKPGVEENIILGVALDGSKRTLPIEDNVPTTASGAKDLAAFNGATMADKNTKRQSPSSPTTSSSE
Homology
BLAST of PI0002717 vs. ExPASy Swiss-Prot
Match: Q56X46 (Mechanosensitive ion channel protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MSL2 PE=2 SV=1)

HSP 1 Score: 674.5 bits (1739), Expect = 1.4e-192
Identity = 413/745 (55.44%), Postives = 496/745 (66.58%), Query Frame = 0

Query: 1   MVLVGSLQLSYHLGPWRNYLHEKNLKFTS-QSYGIRLLNVASSSSLLFQDTWSTHLFSMK 60
           M L G+LQLS+ LG  RN       +F + ++  +R     S+  L        H FS  
Sbjct: 1   MALYGTLQLSHSLGLCRNQ------RFCNPENSAMRRRLHISNGPLSLGVPLGQHGFSNI 60

Query: 61  YPPNY------TVPSRYNVFRCRSSLLTNQPLDPPGMKAAIVTLTRFCNVLGGCPPPVIK 120
              NY      +VP R   FRC S   + + ++ P +KA  V LT+   ++    P V K
Sbjct: 61  LLSNYLRRPICSVPCRTTAFRCHSFSASGKAIE-PAVKAVTVVLTKSHGLMQQF-PFVYK 120

Query: 121 LVPAVCIIIFAVWGLGPFLRYTRSLF--HNDSNWKKSRTYNVMTLYLQPLLLWTGATLIC 180
           LVPAV +++F++WGL PF R  R++    ND+ WKKS TY+VMT Y+QPLLLW GA  IC
Sbjct: 121 LVPAVALLVFSLWGLVPFARQGRNILLNKNDNGWKKSGTYHVMTSYVQPLLLWLGALFIC 180

Query: 181 RALDPVVLRTESSQVVKQRVLNFVRSLSTVLASAYCLSSMIQQAQKFFSESTESSDARNM 240
           RALDPVVL TE+S++VK R+LNFVRSLSTVLA AYCLSS+IQQ QK FSE++  SD RNM
Sbjct: 181 RALDPVVLPTEASKIVKDRLLNFVRSLSTVLAFAYCLSSLIQQTQKLFSETSNPSDTRNM 240

Query: 241 GFQFAWKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMI 300
           GFQFA KA+YSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVL+TLAGREI TNFLSSVMI
Sbjct: 241 GFQFAGKALYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLITLAGREILTNFLSSVMI 300

Query: 301 HATRPFVVNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTMNVVRNLS 360
           HATRPFV+NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREA+HIPNH+FT+NVVRNL+
Sbjct: 301 HATRPFVLNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAIHIPNHKFTVNVVRNLT 360

Query: 361 QKTHWRIKTHIAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVDPENQALL 420
           QKTHWRIKTH+AISHLDVNKINNIVADMRKVLAKNP VEQQRLHRRVFLENV PENQAL 
Sbjct: 361 QKTHWRIKTHLAISHLDVNKINNIVADMRKVLAKNPMVEQQRLHRRVFLENVIPENQALS 420

Query: 421 ILISCFVKTSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDLESVPFSD 480
           ILISCFVKTSH EEYL VKEAI+LDLLRVI HHRARLATPIRT++KM++++D+E+ PF +
Sbjct: 421 ILISCFVKTSHHEEYLGVKEAILLDLLRVISHHRARLATPIRTIRKMYTETDVENTPFGE 480

Query: 481 SIFGQRGATLNRRMLMIEPPYKVYGEDRKQSHSTNRTSRTTGEQNGKPIARSSGDSKAAK 540
           S++G  G T  R +++IEP YK+ GED+  S S NR ++ T EQ  K      G +  +K
Sbjct: 481 SMYG--GVTSRRPLMLIEPAYKINGEDK--SKSQNRAAKPTAEQENK------GSNPKSK 540

Query: 541 ETMSSDRKTEVKTGGDADTKKHFKVSMSASEDKSSNELKHKPSSKSAASTSGMPTFDAKP 600
           ET S D K  VK G           S  +  +K   E   KP  K+              
Sbjct: 541 ETSSPDLKANVKVG----------ESPVSDTNKVPEETVAKPVIKAV------------- 600

Query: 601 TKSDADNSLEDSSPKQSEDSMGSNTQNFKPSLPAVSSPEDVKKPGGTTSAAASQPRIEGE 660
                      S P   +D+  S T+  KP           K+ GGT            +
Sbjct: 601 -----------SKPPTPKDTETSGTE--KPK---------AKRSGGTI-----------K 660

Query: 661 QTTVSNTTVSNPSAKKPGVEENIILGVALDGSKRTLPIEDNV--PTTASGAKDL-----A 720
            T    T  S  SA +  +EENI+LGVAL+GSKRTLPIE+ +  P   + AK+L     +
Sbjct: 661 STKTDETDSSTSSASRSTLEENIVLGVALEGSKRTLPIEEEIHSPPMETDAKELTGARRS 670

Query: 721 AFNGATMADKNTKRQSPSSPTTSSS 730
             NG  +ADK  K  S S P + +S
Sbjct: 721 GGNGPLVADKEQK-DSQSQPNSGAS 670

BLAST of PI0002717 vs. ExPASy Swiss-Prot
Match: Q8L7W1 (Mechanosensitive ion channel protein 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MSL3 PE=1 SV=1)

HSP 1 Score: 611.3 bits (1575), Expect = 1.4e-173
Identity = 358/670 (53.43%), Postives = 447/670 (66.72%), Query Frame = 0

Query: 34  IRLLNVASSSSLLFQDTWSTHLFSMKYPPNYTVPSRYNVFRCRSSLLTNQPLDPPGMKAA 93
           + L     SS    QD WS  L          V SR N F CRS+L      + P +K+ 
Sbjct: 35  VYLTRTGLSSCATRQDVWSLQLLESLSGSIVPVSSRCNAFVCRSALSPGNGNEGPILKST 94

Query: 94  IVTLTRFCNVLGGCPPPVIKLVPAVCIIIFAVWGLGPFLRYTR-SLFH--NDSNWKKSRT 153
            V  TR  + LGG  P ++KL+PAV I+ FA WGL P LR  R +LF   ND+N +KS T
Sbjct: 95  AVIFTRVYDALGG-NPHLVKLIPAVGILAFATWGLRPLLRLARTTLFEKGNDANSQKSST 154

Query: 154 YNVMTLYLQPLLLWTGATLICRALDPVVLRTESSQVVKQRVLNFVRSLSTVLASAYCLSS 213
             ++  YLQPLLLW+GA L+CR LDP+VL + + Q +KQR+L F RS+STVLA + CLSS
Sbjct: 155 QYIVVSYLQPLLLWSGAILLCRTLDPIVLPSSAGQAIKQRLLIFARSISTVLAFSCCLSS 214

Query: 214 MIQQAQKFFSESTESSDARNMGFQFAWKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLG 273
           ++QQ QKFF E+   +D RNMGF FA KAVY+A WVAA SLFMELLGFSTQKWLTAGGLG
Sbjct: 215 LLQQVQKFFMETNNPADTRNMGFSFAGKAVYTAAWVAAASLFMELLGFSTQKWLTAGGLG 274

Query: 274 TVLLTLAGREIFTNFLSSVMIHATRPFVVNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGE 333
           TVLLTLAGREI TNFLSS+MIHATRPFV+NEWIQTKI GYEVSGTVE VGWWSPTIIRG+
Sbjct: 275 TVLLTLAGREILTNFLSSIMIHATRPFVLNEWIQTKIGGYEVSGTVEQVGWWSPTIIRGD 334

Query: 334 DREAVHIPNHQFTMNVVRNLSQKTHWRIKTHIAISHLDVNKINNIVADMRKVLAKNPQVE 393
           DREAVHIPNHQF++N+VRNL+QKTHWRIKTH+AISHLDV+KINNIVADMRKVL+KNPQ+E
Sbjct: 335 DREAVHIPNHQFSVNIVRNLTQKTHWRIKTHLAISHLDVSKINNIVADMRKVLSKNPQIE 394

Query: 394 QQRLHRRVFLENVDPENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHHRARLAT 453
           QQ++HRRVFLE++DPENQAL ILISCFVKTS FEEYLCVKEA++LDLL VIRHH ARLAT
Sbjct: 395 QQKIHRRVFLEDIDPENQALRILISCFVKTSRFEEYLCVKEAVLLDLLTVIRHHGARLAT 454

Query: 454 PIRTMQKMHSDSDLESVPFSDSIFGQRGATLNRRMLMIEPPYKVYGEDRKQSHSTNRTSR 513
           PIRT+Q+M +++++++  FSD +F Q  A +NRR ++IEP YK+  +D  +S      S 
Sbjct: 455 PIRTVQRMRNEAEVDTAGFSDIVFNQ--AAMNRRYMLIEPSYKINSDDNSKS-----PSP 514

Query: 514 TTGEQNGKPIARSSGDSKAAKETMSSDRKTEVKTGGDADTKKHFKVSMSASEDKSSNELK 573
           + G+++  P     G     ++      +T+ +T  +         S+  S  K  N+ K
Sbjct: 515 SPGQKSPSP-----GQKSEERDLQEEPSETKAETENNG--------SVPVSNAKKENQ-K 574

Query: 574 HKPSSKSAASTSGMPTFDAKPTKSDADNSLEDSSPKQSEDSMGSNTQNFKPSLPAVSSPE 633
               S S   T G  T       S +D  +   S ++ ++S+G      K     VS  E
Sbjct: 575 AALGSNSNTGTKGSST-------STSDQPVAQKSEEKKKESVG---DPHKAEKDEVSDDE 634

Query: 634 DVKKPGGTTSAAASQPRIEGEQTTVSNTTVSNPSAKKPGVEENIILGVALDGSKRTLPIE 693
              +    + A     +  GE    S       S     +EEN++LGVALDGSKRTLPI+
Sbjct: 635 ATIEQTLKSKAKQGSEKNNGE----SKARDGGGSGTSSLLEENLVLGVALDGSKRTLPID 666

Query: 694 DNVPTTASGA 701
           +     ASGA
Sbjct: 695 EE--HKASGA 666

BLAST of PI0002717 vs. ExPASy Swiss-Prot
Match: P0AEB6 (Low conductance mechanosensitive channel YnaI OS=Escherichia coli O157:H7 OX=83334 GN=ynaI PE=3 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 1.1e-21
Identity = 79/292 (27.05%), Postives = 149/292 (51.03%), Query Frame = 0

Query: 168 TLICRALDPV---VLRTESSQVVKQRVLNFV-RSLSTVLASAYCLSSMIQQAQKFFSEST 227
           T+I  A+  +   V+     + +   V++F+  SL  V+ +      + Q  ++   +  
Sbjct: 51  TIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFEKQQIKKGR 110

Query: 228 ESSDARNMGFQFAWKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFT 287
           + + AR M      + +   + V  V L+ E  G S    LT GG+G + + +AG++I +
Sbjct: 111 DITSARIMS-----RIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILS 170

Query: 288 NFLSSVMIHATRPFVVNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFT 347
           NF S +M++  RPF + +WI++     E  GTV  +G W  T I   D   +++PN  F+
Sbjct: 171 NFFSGIMLYFDRPFSIGDWIRSPDRNIE--GTVAEIG-WRITKITTFDNRPLYVPNSLFS 230

Query: 348 MNVVRNLSQKTHWRIKTHIAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENV 407
              V N  + T+ RI T I + + D  K+  IV  +R++L  +P ++Q++     F +  
Sbjct: 231 SISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYFNQFA 290

Query: 408 DPENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTM 456
           D    +L I++ CF KT+ + E+L  ++ + L ++ +++ H A  A P +T+
Sbjct: 291 D---SSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTL 331

BLAST of PI0002717 vs. ExPASy Swiss-Prot
Match: P0AEB5 (Low conductance mechanosensitive channel YnaI OS=Escherichia coli (strain K12) OX=83333 GN=ynaI PE=1 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 1.1e-21
Identity = 79/292 (27.05%), Postives = 149/292 (51.03%), Query Frame = 0

Query: 168 TLICRALDPV---VLRTESSQVVKQRVLNFV-RSLSTVLASAYCLSSMIQQAQKFFSEST 227
           T+I  A+  +   V+     + +   V++F+  SL  V+ +      + Q  ++   +  
Sbjct: 51  TIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFEKQQIKKGR 110

Query: 228 ESSDARNMGFQFAWKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFT 287
           + + AR M      + +   + V  V L+ E  G S    LT GG+G + + +AG++I +
Sbjct: 111 DITSARIMS-----RIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILS 170

Query: 288 NFLSSVMIHATRPFVVNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFT 347
           NF S +M++  RPF + +WI++     E  GTV  +G W  T I   D   +++PN  F+
Sbjct: 171 NFFSGIMLYFDRPFSIGDWIRSPDRNIE--GTVAEIG-WRITKITTFDNRPLYVPNSLFS 230

Query: 348 MNVVRNLSQKTHWRIKTHIAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENV 407
              V N  + T+ RI T I + + D  K+  IV  +R++L  +P ++Q++     F +  
Sbjct: 231 SISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYFNQFA 290

Query: 408 DPENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTM 456
           D    +L I++ CF KT+ + E+L  ++ + L ++ +++ H A  A P +T+
Sbjct: 291 D---SSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTL 331

BLAST of PI0002717 vs. ExPASy Swiss-Prot
Match: O66994 (Uncharacterized MscS family protein aq_812 OS=Aquifex aeolicus (strain VF5) OX=224324 GN=aq_812 PE=3 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 2.8e-12
Identity = 65/240 (27.08%), Postives = 122/240 (50.83%), Query Frame = 0

Query: 217 KFFSESTESSDARNMGFQFAWKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTL 276
           +FF +      A  +G  F  K + + V V   +  ++  G +    L + GL  + ++L
Sbjct: 130 EFFVKVGGKDFAEEVG-DFILKILKAFVVVIVGASLLQEWGVNIGAILASVGLLGLAVSL 189

Query: 277 AGREIFTNFLSSVMIHATRPFVVNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVH 336
           A ++ F N LS ++I   +P  V E ++ K    +  G+VE +G  S T IR  D+  V 
Sbjct: 190 AAKDTFENILSGLIILLDKPVKVGETVKVK----DFMGSVEDIGLRS-TKIRTFDKSLVT 249

Query: 337 IPNHQFTMNVVRNLSQKTHWRIKTHIAISH-LDVNKINNIVADMRKVLAKNPQVEQQRLH 396
           IPN     N V N +++   R++ +I + +     ++ NI+ ++R++L ++P V +    
Sbjct: 250 IPNRDIVNNHVENFTRRNKRRVRFYIGVVYSTKREQLENILKEIRELLKEHPGVAKDEKF 309

Query: 397 RRVFLENVDPENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTM 456
             V+ EN    + +L ILI  +  T+ +EEYL + E I L ++ ++  + +  A P R++
Sbjct: 310 -YVYFENYG--DSSLNILIQYYANTNDYEEYLKIIEDINLKIMEIVEKNGSSFAFPSRSV 360

BLAST of PI0002717 vs. ExPASy TrEMBL
Match: A0A1S3BT67 (mechanosensitive ion channel protein 2, chloroplastic-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103493276 PE=4 SV=1)

HSP 1 Score: 1317.8 bits (3409), Expect = 0.0e+00
Identity = 692/731 (94.66%), Postives = 702/731 (96.03%), Query Frame = 0

Query: 1   MVLVGSLQLSYHLGPWRNYLHEKNLKFTSQSYGIRLLNVASSSSLLFQ-DTWSTHLFSMK 60
           MVLVGSLQLSYHLGPWRNYLHE+NLKFTSQSYGIRLLNVASSSSLLFQ DTWSTHLFSMK
Sbjct: 1   MVLVGSLQLSYHLGPWRNYLHEENLKFTSQSYGIRLLNVASSSSLLFQKDTWSTHLFSMK 60

Query: 61  YPPNYTVPSRYNVFRCRSSLLTNQPLDPPGMKAAIVTLTRFCNVLGGCPPPVIKLVPAVC 120
           YPPNYTVPSRYNVFRC+SSL+TNQPLD PGMKA+IVTLTRFCNVLGGCPP VIKLVP VC
Sbjct: 61  YPPNYTVPSRYNVFRCQSSLMTNQPLDLPGMKASIVTLTRFCNVLGGCPPSVIKLVPPVC 120

Query: 121 IIIFAVWGLGPFLRYTRSLFHNDSNWKKSRTYNVMTLYLQPLLLWTGATLICRALDPVVL 180
           IIIFAVWGLGPFLR+TRSLFHNDSNWKKSRTYNVMTLYLQPLLLWTGATLICRALDPVVL
Sbjct: 121 IIIFAVWGLGPFLRHTRSLFHNDSNWKKSRTYNVMTLYLQPLLLWTGATLICRALDPVVL 180

Query: 181 RTESSQVVKQRVLNFVRSLSTVLASAYCLSSMIQQAQKFFSESTESSDARNMGFQFAWKA 240
           RTESSQVVKQRVLNFVRSLSTVLASAYCLSSMIQQAQKFFSE+TESSDARNMGFQFAWKA
Sbjct: 181 RTESSQVVKQRVLNFVRSLSTVLASAYCLSSMIQQAQKFFSENTESSDARNMGFQFAWKA 240

Query: 241 VYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 300
           VYSAVWVAA+SLFMELLGFSTQK LTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV
Sbjct: 241 VYSAVWVAAISLFMELLGFSTQKLLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 300

Query: 301 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTMNVVRNLSQKTHWRIK 360
           NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTMNVVRNLSQKTHWRIK
Sbjct: 301 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTMNVVRNLSQKTHWRIK 360

Query: 361 THIAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVDPENQALLILISCFVK 420
           TH+AISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVD ENQALLILISCFVK
Sbjct: 361 THLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVDSENQALLILISCFVK 420

Query: 421 TSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGQRGA 480
           TSHFEEYLCVKEAIILDLLRVIRHH ARLATPIRTMQKMHSDSDLESVPFSDSIFG RGA
Sbjct: 421 TSHFEEYLCVKEAIILDLLRVIRHHGARLATPIRTMQKMHSDSDLESVPFSDSIFGHRGA 480

Query: 481 TLNRRMLMIEPPYKVYGEDRKQSHSTNRTSRTTGEQNGKPIARSSGDSKAAKETMSSDRK 540
           TLNRRMLMIEPPYKV+GEDRKQSHS  RTSRTTGEQNGKPIARSSGDSKAAKETMSSDRK
Sbjct: 481 TLNRRMLMIEPPYKVHGEDRKQSHS--RTSRTTGEQNGKPIARSSGDSKAAKETMSSDRK 540

Query: 541 TEVKTGGDADTKKHFKVSMSASEDKSSNELKHKPSSKSAASTSGMPTFDAKPTKSDADNS 600
           TEVKTGGDADTKKH KVSMS SEDKSSNELKHKPSSKSAASTS MPT DAKPTKSDADNS
Sbjct: 541 TEVKTGGDADTKKHSKVSMSVSEDKSSNELKHKPSSKSAASTSDMPTSDAKPTKSDADNS 600

Query: 601 LEDSSPKQSEDSMGSNTQNFKPSLPAVSSPEDVKKPGGTTSAAASQPRIEGEQTTVSNTT 660
           LEDSSPKQSEDS GSN QNFKPSLPAVSSPEDVKKPGGTTSAAASQ RI GEQ     TT
Sbjct: 601 LEDSSPKQSEDSFGSNMQNFKPSLPAVSSPEDVKKPGGTTSAAASQQRIVGEQ-----TT 660

Query: 661 VSNPSAKKPGVEENIILGVALDGSKRTLPIEDNVPTTASGAKDLAAFNGATMADKNTKRQ 720
           V NPS KKPGVEENIILGVALDG KRTLPIEDN+PT ASGAKDLA FNGATMADKNTK Q
Sbjct: 661 VPNPSTKKPGVEENIILGVALDGPKRTLPIEDNLPTAASGAKDLAVFNGATMADKNTKCQ 720

Query: 721 SPSSPTTSSSE 731
           SPSSPTTSSSE
Sbjct: 721 SPSSPTTSSSE 724

BLAST of PI0002717 vs. ExPASy TrEMBL
Match: A0A0A0L5I6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G114440 PE=4 SV=1)

HSP 1 Score: 1314.7 bits (3401), Expect = 0.0e+00
Identity = 687/731 (93.98%), Postives = 702/731 (96.03%), Query Frame = 0

Query: 1   MVLVGSLQLSYHLGPWRNYLHEKNLKFTSQSYGIRLLNVASSSSLLFQ-DTWSTHLFSMK 60
           MVLVGSLQLSYHL PWRNYLHE+NLKFTSQ YGIRLLNV+SSSSLLFQ DTWSTHLFSMK
Sbjct: 1   MVLVGSLQLSYHLRPWRNYLHEENLKFTSQRYGIRLLNVSSSSSLLFQKDTWSTHLFSMK 60

Query: 61  YPPNYTVPSRYNVFRCRSSLLTNQPLDPPGMKAAIVTLTRFCNVLGGCPPPVIKLVPAVC 120
           YPPNYTVPSRYNVFRC+SSL+TNQPLDPPGMKAAIVTL RFCNVLGGCPPPVIKL+P VC
Sbjct: 61  YPPNYTVPSRYNVFRCQSSLMTNQPLDPPGMKAAIVTLMRFCNVLGGCPPPVIKLLPPVC 120

Query: 121 IIIFAVWGLGPFLRYTRSLFHNDSNWKKSRTYNVMTLYLQPLLLWTGATLICRALDPVVL 180
           II+FAVWGLGPFL YTRSLFHNDSNWKKSRTYNVMTL+LQPLLLWTGATLICRALDP+VL
Sbjct: 121 IILFAVWGLGPFLCYTRSLFHNDSNWKKSRTYNVMTLFLQPLLLWTGATLICRALDPIVL 180

Query: 181 RTESSQVVKQRVLNFVRSLSTVLASAYCLSSMIQQAQKFFSESTESSDARNMGFQFAWKA 240
           RTESSQVVKQRVLNFVRSLSTVLASAYCLSSMIQQAQKFFSESTESSDARNMGFQFAWKA
Sbjct: 181 RTESSQVVKQRVLNFVRSLSTVLASAYCLSSMIQQAQKFFSESTESSDARNMGFQFAWKA 240

Query: 241 VYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 300
           VYSAVWVAA+SLFMELLGFSTQK LTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV
Sbjct: 241 VYSAVWVAAISLFMELLGFSTQKLLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 300

Query: 301 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTMNVVRNLSQKTHWRIK 360
           NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTMNVVRNLSQKTHWRIK
Sbjct: 301 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTMNVVRNLSQKTHWRIK 360

Query: 361 THIAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVDPENQALLILISCFVK 420
           TH+AISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVD ENQALLILISCFVK
Sbjct: 361 THLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVDSENQALLILISCFVK 420

Query: 421 TSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGQRGA 480
           TSHFEEYLCVKEAIILDLLRVIRHH ARLATPIRTMQKMHSDSDLE+VPFSDSIFG RGA
Sbjct: 421 TSHFEEYLCVKEAIILDLLRVIRHHGARLATPIRTMQKMHSDSDLENVPFSDSIFGHRGA 480

Query: 481 TLNRRMLMIEPPYKVYGEDRKQSHSTNRTSRTTGEQNGKPIARSSGDSKAAKETMSSDRK 540
           TLNRRMLMIEPPYKVYGEDRKQSHS  RTSRTTGEQNGKPIARSSGDSKAAKETMSSDRK
Sbjct: 481 TLNRRMLMIEPPYKVYGEDRKQSHS--RTSRTTGEQNGKPIARSSGDSKAAKETMSSDRK 540

Query: 541 TEVKTGGDADTKKHFKVSMSASEDKSSNELKHKPSSKSAASTSGMPTFDAKPTKSDADNS 600
            EVKTGGD DTKKH K SMSASEDKSSNELK+KPSSKSAASTS  PT +AKPTKSDADNS
Sbjct: 541 IEVKTGGDGDTKKHSKASMSASEDKSSNELKYKPSSKSAASTSDTPTSNAKPTKSDADNS 600

Query: 601 LEDSSPKQSEDSMGSNTQNFKPSLPAVSSPEDVKKPGGTTSAAASQPRIEGEQTTVSNTT 660
           LEDS PKQS+DS+GSN QNFKPSLPAVSSPEDVKKPGGTTSAAASQPRIEGEQ     TT
Sbjct: 601 LEDSRPKQSKDSLGSNMQNFKPSLPAVSSPEDVKKPGGTTSAAASQPRIEGEQ-----TT 660

Query: 661 VSNPSAKKPGVEENIILGVALDGSKRTLPIEDNVPTTASGAKDLAAFNGATMADKNTKRQ 720
           VSNPS KKPGVEENIILGVALDGSKRTLPIEDNVPTTASGAKDLAAFNGAT  DKNTKRQ
Sbjct: 661 VSNPSTKKPGVEENIILGVALDGSKRTLPIEDNVPTTASGAKDLAAFNGATTTDKNTKRQ 720

Query: 721 SPSSPTTSSSE 731
           SPSSPTTSSSE
Sbjct: 721 SPSSPTTSSSE 724

BLAST of PI0002717 vs. ExPASy TrEMBL
Match: A0A5A7UYX2 (Mechanosensitive ion channel protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold22G002750 PE=4 SV=1)

HSP 1 Score: 1307.4 bits (3382), Expect = 0.0e+00
Identity = 692/748 (92.51%), Postives = 702/748 (93.85%), Query Frame = 0

Query: 1   MVLVGSLQLSYHLGPWRNYLHEKNLKFTSQSYGIRLLNVASSSSLLFQ-DTWSTHLFSMK 60
           MVLVGSLQLSYHLGPWRNYLHE+NLKFTSQSYGIRLLNVASSSSLLFQ DTWSTHLFSMK
Sbjct: 1   MVLVGSLQLSYHLGPWRNYLHEENLKFTSQSYGIRLLNVASSSSLLFQKDTWSTHLFSMK 60

Query: 61  YPPNYTVPSRYNVFRCRSSLLTNQPLDPPGMKAAIVTLTRFCNVLGGCPPPVIKLVPAVC 120
           YPPNYTVPSRYNVFRC+SSL+TNQPLD PGMKA+IVTLTRFCNVLGGCPP VIKLVP VC
Sbjct: 61  YPPNYTVPSRYNVFRCQSSLMTNQPLDLPGMKASIVTLTRFCNVLGGCPPSVIKLVPPVC 120

Query: 121 IIIFAVWGLGPFLRYTRSLFHNDSNWKKSRTYNVMTLYLQPLLLWTGATLICRALDPVVL 180
           IIIFAVWGLGPFLR+TRSLFHNDSNWKKSRTYNVMTLYLQPLLLWTGATLICRALDPVVL
Sbjct: 121 IIIFAVWGLGPFLRHTRSLFHNDSNWKKSRTYNVMTLYLQPLLLWTGATLICRALDPVVL 180

Query: 181 RTESSQVVKQRVLNFVRSLSTVLASAYCLSSMIQQAQKFFSESTESSDARNMGFQFAWKA 240
           RTESSQVVKQRVLNFVRSLSTVLASAYCLSSMIQQAQKFFSE+TESSDARNMGFQFAWKA
Sbjct: 181 RTESSQVVKQRVLNFVRSLSTVLASAYCLSSMIQQAQKFFSENTESSDARNMGFQFAWKA 240

Query: 241 VYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 300
           VYSAVWVAA+SLFMELLGFSTQK LTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV
Sbjct: 241 VYSAVWVAAISLFMELLGFSTQKLLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 300

Query: 301 NEWIQTKIEGYEVSGTVE-----------------HVGWWSPTIIRGEDREAVHIPNHQF 360
           NEWIQTKIEGYEVSGTVE                 HVGWWSPTIIRGEDREAVHIPNHQF
Sbjct: 301 NEWIQTKIEGYEVSGTVEVCMFLLNMYHKLLMSKKHVGWWSPTIIRGEDREAVHIPNHQF 360

Query: 361 TMNVVRNLSQKTHWRIKTHIAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLEN 420
           TMNVVRNLSQKTHWRIKTH+AISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLEN
Sbjct: 361 TMNVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLEN 420

Query: 421 VDPENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDS 480
           VD ENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHH ARLATPIRTMQKMHSDS
Sbjct: 421 VDSENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHHGARLATPIRTMQKMHSDS 480

Query: 481 DLESVPFSDSIFGQRGATLNRRMLMIEPPYKVYGEDRKQSHSTNRTSRTTGEQNGKPIAR 540
           DLESVPFSDSIFG RGATLNRRMLMIEPPYKV+GEDRKQSHS  RTSRTTGEQNGKPIAR
Sbjct: 481 DLESVPFSDSIFGHRGATLNRRMLMIEPPYKVHGEDRKQSHS--RTSRTTGEQNGKPIAR 540

Query: 541 SSGDSKAAKETMSSDRKTEVKTGGDADTKKHFKVSMSASEDKSSNELKHKPSSKSAASTS 600
           SSGDSKAAKETMSSDRKTEVKTGGDADTKKH KVSMS SEDKSSNELKHKPSSKSAASTS
Sbjct: 541 SSGDSKAAKETMSSDRKTEVKTGGDADTKKHSKVSMSVSEDKSSNELKHKPSSKSAASTS 600

Query: 601 GMPTFDAKPTKSDADNSLEDSSPKQSEDSMGSNTQNFKPSLPAVSSPEDVKKPGGTTSAA 660
            MPT DAKPTKSDADNSLEDSSPKQSEDS GSN QNFKPSLP VSSPEDVKKPGGTTSAA
Sbjct: 601 DMPTSDAKPTKSDADNSLEDSSPKQSEDSFGSNMQNFKPSLPTVSSPEDVKKPGGTTSAA 660

Query: 661 ASQPRIEGEQTTVSNTTVSNPSAKKPGVEENIILGVALDGSKRTLPIEDNVPTTASGAKD 720
           ASQ RI GEQ     TTVSNPS KKPGVEENIILGVALDG KRTLPIEDN+PT ASGAKD
Sbjct: 661 ASQQRIVGEQ-----TTVSNPSTKKPGVEENIILGVALDGPKRTLPIEDNLPTAASGAKD 720

Query: 721 LAAFNGATMADKNTKRQSPSSPTTSSSE 731
           LA FNGATMADKNTK QSPSSPTTSSSE
Sbjct: 721 LAVFNGATMADKNTKCQSPSSPTTSSSE 741

BLAST of PI0002717 vs. ExPASy TrEMBL
Match: A0A1S3BUC5 (mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103493276 PE=4 SV=1)

HSP 1 Score: 1307.0 bits (3381), Expect = 0.0e+00
Identity = 692/748 (92.51%), Postives = 702/748 (93.85%), Query Frame = 0

Query: 1   MVLVGSLQLSYHLGPWRNYLHEKNLKFTSQSYGIRLLNVASSSSLLFQ-DTWSTHLFSMK 60
           MVLVGSLQLSYHLGPWRNYLHE+NLKFTSQSYGIRLLNVASSSSLLFQ DTWSTHLFSMK
Sbjct: 1   MVLVGSLQLSYHLGPWRNYLHEENLKFTSQSYGIRLLNVASSSSLLFQKDTWSTHLFSMK 60

Query: 61  YPPNYTVPSRYNVFRCRSSLLTNQPLDPPGMKAAIVTLTRFCNVLGGCPPPVIKLVPAVC 120
           YPPNYTVPSRYNVFRC+SSL+TNQPLD PGMKA+IVTLTRFCNVLGGCPP VIKLVP VC
Sbjct: 61  YPPNYTVPSRYNVFRCQSSLMTNQPLDLPGMKASIVTLTRFCNVLGGCPPSVIKLVPPVC 120

Query: 121 IIIFAVWGLGPFLRYTRSLFHNDSNWKKSRTYNVMTLYLQPLLLWTGATLICRALDPVVL 180
           IIIFAVWGLGPFLR+TRSLFHNDSNWKKSRTYNVMTLYLQPLLLWTGATLICRALDPVVL
Sbjct: 121 IIIFAVWGLGPFLRHTRSLFHNDSNWKKSRTYNVMTLYLQPLLLWTGATLICRALDPVVL 180

Query: 181 RTESSQVVKQRVLNFVRSLSTVLASAYCLSSMIQQAQKFFSESTESSDARNMGFQFAWKA 240
           RTESSQVVKQRVLNFVRSLSTVLASAYCLSSMIQQAQKFFSE+TESSDARNMGFQFAWKA
Sbjct: 181 RTESSQVVKQRVLNFVRSLSTVLASAYCLSSMIQQAQKFFSENTESSDARNMGFQFAWKA 240

Query: 241 VYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 300
           VYSAVWVAA+SLFMELLGFSTQK LTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV
Sbjct: 241 VYSAVWVAAISLFMELLGFSTQKLLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 300

Query: 301 NEWIQTKIEGYEVSGTVE-----------------HVGWWSPTIIRGEDREAVHIPNHQF 360
           NEWIQTKIEGYEVSGTVE                 HVGWWSPTIIRGEDREAVHIPNHQF
Sbjct: 301 NEWIQTKIEGYEVSGTVEVCMFLLNMYHKLLMSKKHVGWWSPTIIRGEDREAVHIPNHQF 360

Query: 361 TMNVVRNLSQKTHWRIKTHIAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLEN 420
           TMNVVRNLSQKTHWRIKTH+AISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLEN
Sbjct: 361 TMNVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLEN 420

Query: 421 VDPENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDS 480
           VD ENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHH ARLATPIRTMQKMHSDS
Sbjct: 421 VDSENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHHGARLATPIRTMQKMHSDS 480

Query: 481 DLESVPFSDSIFGQRGATLNRRMLMIEPPYKVYGEDRKQSHSTNRTSRTTGEQNGKPIAR 540
           DLESVPFSDSIFG RGATLNRRMLMIEPPYKV+GEDRKQSHS  RTSRTTGEQNGKPIAR
Sbjct: 481 DLESVPFSDSIFGHRGATLNRRMLMIEPPYKVHGEDRKQSHS--RTSRTTGEQNGKPIAR 540

Query: 541 SSGDSKAAKETMSSDRKTEVKTGGDADTKKHFKVSMSASEDKSSNELKHKPSSKSAASTS 600
           SSGDSKAAKETMSSDRKTEVKTGGDADTKKH KVSMS SEDKSSNELKHKPSSKSAASTS
Sbjct: 541 SSGDSKAAKETMSSDRKTEVKTGGDADTKKHSKVSMSVSEDKSSNELKHKPSSKSAASTS 600

Query: 601 GMPTFDAKPTKSDADNSLEDSSPKQSEDSMGSNTQNFKPSLPAVSSPEDVKKPGGTTSAA 660
            MPT DAKPTKSDADNSLEDSSPKQSEDS GSN QNFKPSLPAVSSPEDVKKPGGTTSAA
Sbjct: 601 DMPTSDAKPTKSDADNSLEDSSPKQSEDSFGSNMQNFKPSLPAVSSPEDVKKPGGTTSAA 660

Query: 661 ASQPRIEGEQTTVSNTTVSNPSAKKPGVEENIILGVALDGSKRTLPIEDNVPTTASGAKD 720
           ASQ RI GEQ     TTV NPS KKPGVEENIILGVALDG KRTLPIEDN+PT ASGAKD
Sbjct: 661 ASQQRIVGEQ-----TTVPNPSTKKPGVEENIILGVALDGPKRTLPIEDNLPTAASGAKD 720

Query: 721 LAAFNGATMADKNTKRQSPSSPTTSSSE 731
           LA FNGATMADKNTK QSPSSPTTSSSE
Sbjct: 721 LAVFNGATMADKNTKCQSPSSPTTSSSE 741

BLAST of PI0002717 vs. ExPASy TrEMBL
Match: A0A5D3D4R2 (Mechanosensitive ion channel protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold119G00320 PE=4 SV=1)

HSP 1 Score: 1306.6 bits (3380), Expect = 0.0e+00
Identity = 692/747 (92.64%), Postives = 702/747 (93.98%), Query Frame = 0

Query: 1   MVLVGSLQLSYHLGPWRNYLHEKNLKFTSQSYGIRLLNVASSSSLLFQ-DTWSTHLFSMK 60
           MVLVGSLQLSYHLGPWRNYLHE+NLKFTSQSY IRLLNVASSSSLLFQ DTWSTHLFSMK
Sbjct: 1   MVLVGSLQLSYHLGPWRNYLHEENLKFTSQSYDIRLLNVASSSSLLFQKDTWSTHLFSMK 60

Query: 61  YPPNYTVPSRYNVFRCRSSLLTNQPLDPPGMKAAIVTLTRFCNVLGGCPPPVIKLVPAVC 120
           YPPNYTVPSRYNVFRC+SSL+TNQPLD PGMKA+IVTLTRFCNVLGGCPP VIKLVP VC
Sbjct: 61  YPPNYTVPSRYNVFRCQSSLMTNQPLDLPGMKASIVTLTRFCNVLGGCPPSVIKLVPPVC 120

Query: 121 IIIFAVWGLGPFLRYTRSLFHNDSNWKKSRTYNVMTLYLQPLLLWTGATLICRALDPVVL 180
           IIIFAVWGLGPFLR+TRSLFHNDSNWKKSRTYNVMTLYLQPLLLWTGATLICRALDPVVL
Sbjct: 121 IIIFAVWGLGPFLRHTRSLFHNDSNWKKSRTYNVMTLYLQPLLLWTGATLICRALDPVVL 180

Query: 181 RTESSQVVKQRVLNFVRSLSTVLASAYCLSSMIQQAQKFFSESTESSDARNMGFQFAWKA 240
           RTESSQVVKQRVLNFVRSLSTVLASAYCLSSMIQQAQKFFSE+TESSDARNMGFQFAWKA
Sbjct: 181 RTESSQVVKQRVLNFVRSLSTVLASAYCLSSMIQQAQKFFSENTESSDARNMGFQFAWKA 240

Query: 241 VYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 300
           VYSAVWVAA+SLFMELLGFSTQK LTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV
Sbjct: 241 VYSAVWVAAISLFMELLGFSTQKLLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 300

Query: 301 NEWIQTKIEGYEVSGTVE----------------HVGWWSPTIIRGEDREAVHIPNHQFT 360
           NEWIQTKIEGYEVSGTVE                HVGWWSPTIIRGEDREAVHIPNHQFT
Sbjct: 301 NEWIQTKIEGYEVSGTVEVCMFLNMYHKLLMSKKHVGWWSPTIIRGEDREAVHIPNHQFT 360

Query: 361 MNVVRNLSQKTHWRIKTHIAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENV 420
           MNVVRNLSQKTHWRIKTH+AISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENV
Sbjct: 361 MNVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENV 420

Query: 421 DPENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSD 480
           D ENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHH ARLATPIRTMQKMHSDSD
Sbjct: 421 DSENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHHGARLATPIRTMQKMHSDSD 480

Query: 481 LESVPFSDSIFGQRGATLNRRMLMIEPPYKVYGEDRKQSHSTNRTSRTTGEQNGKPIARS 540
           LESVPFSDSIFG RGATLNRRMLMIEPPYKV+GEDRKQSHS  RTSRTTGEQNGKPIARS
Sbjct: 481 LESVPFSDSIFGHRGATLNRRMLMIEPPYKVHGEDRKQSHS--RTSRTTGEQNGKPIARS 540

Query: 541 SGDSKAAKETMSSDRKTEVKTGGDADTKKHFKVSMSASEDKSSNELKHKPSSKSAASTSG 600
           SGDSKAAKETMSSDRKTEVKTGGDADTKKH KVSMS SEDKSSNELKHKPSSKSAASTS 
Sbjct: 541 SGDSKAAKETMSSDRKTEVKTGGDADTKKHSKVSMSVSEDKSSNELKHKPSSKSAASTSD 600

Query: 601 MPTFDAKPTKSDADNSLEDSSPKQSEDSMGSNTQNFKPSLPAVSSPEDVKKPGGTTSAAA 660
           MPT DAKPTKSDADNSLEDSSPKQSEDS GSN QNFKPSLPAVSSPEDVKKPGGTTSAAA
Sbjct: 601 MPTSDAKPTKSDADNSLEDSSPKQSEDSFGSNMQNFKPSLPAVSSPEDVKKPGGTTSAAA 660

Query: 661 SQPRIEGEQTTVSNTTVSNPSAKKPGVEENIILGVALDGSKRTLPIEDNVPTTASGAKDL 720
           SQ RI GEQ     TTVSNPS KKPGVEENIILGVALDG KRTLPIEDN+PT ASGAKDL
Sbjct: 661 SQQRIVGEQ-----TTVSNPSTKKPGVEENIILGVALDGPKRTLPIEDNLPTAASGAKDL 720

Query: 721 AAFNGATMADKNTKRQSPSSPTTSSSE 731
           A FNGATMADKNTK QSPSSPTTSSSE
Sbjct: 721 AVFNGATMADKNTKCQSPSSPTTSSSE 740

BLAST of PI0002717 vs. NCBI nr
Match: XP_008452175.1 (PREDICTED: mechanosensitive ion channel protein 2, chloroplastic-like isoform X2 [Cucumis melo])

HSP 1 Score: 1317.8 bits (3409), Expect = 0.0e+00
Identity = 692/731 (94.66%), Postives = 702/731 (96.03%), Query Frame = 0

Query: 1   MVLVGSLQLSYHLGPWRNYLHEKNLKFTSQSYGIRLLNVASSSSLLFQ-DTWSTHLFSMK 60
           MVLVGSLQLSYHLGPWRNYLHE+NLKFTSQSYGIRLLNVASSSSLLFQ DTWSTHLFSMK
Sbjct: 1   MVLVGSLQLSYHLGPWRNYLHEENLKFTSQSYGIRLLNVASSSSLLFQKDTWSTHLFSMK 60

Query: 61  YPPNYTVPSRYNVFRCRSSLLTNQPLDPPGMKAAIVTLTRFCNVLGGCPPPVIKLVPAVC 120
           YPPNYTVPSRYNVFRC+SSL+TNQPLD PGMKA+IVTLTRFCNVLGGCPP VIKLVP VC
Sbjct: 61  YPPNYTVPSRYNVFRCQSSLMTNQPLDLPGMKASIVTLTRFCNVLGGCPPSVIKLVPPVC 120

Query: 121 IIIFAVWGLGPFLRYTRSLFHNDSNWKKSRTYNVMTLYLQPLLLWTGATLICRALDPVVL 180
           IIIFAVWGLGPFLR+TRSLFHNDSNWKKSRTYNVMTLYLQPLLLWTGATLICRALDPVVL
Sbjct: 121 IIIFAVWGLGPFLRHTRSLFHNDSNWKKSRTYNVMTLYLQPLLLWTGATLICRALDPVVL 180

Query: 181 RTESSQVVKQRVLNFVRSLSTVLASAYCLSSMIQQAQKFFSESTESSDARNMGFQFAWKA 240
           RTESSQVVKQRVLNFVRSLSTVLASAYCLSSMIQQAQKFFSE+TESSDARNMGFQFAWKA
Sbjct: 181 RTESSQVVKQRVLNFVRSLSTVLASAYCLSSMIQQAQKFFSENTESSDARNMGFQFAWKA 240

Query: 241 VYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 300
           VYSAVWVAA+SLFMELLGFSTQK LTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV
Sbjct: 241 VYSAVWVAAISLFMELLGFSTQKLLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 300

Query: 301 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTMNVVRNLSQKTHWRIK 360
           NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTMNVVRNLSQKTHWRIK
Sbjct: 301 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTMNVVRNLSQKTHWRIK 360

Query: 361 THIAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVDPENQALLILISCFVK 420
           TH+AISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVD ENQALLILISCFVK
Sbjct: 361 THLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVDSENQALLILISCFVK 420

Query: 421 TSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGQRGA 480
           TSHFEEYLCVKEAIILDLLRVIRHH ARLATPIRTMQKMHSDSDLESVPFSDSIFG RGA
Sbjct: 421 TSHFEEYLCVKEAIILDLLRVIRHHGARLATPIRTMQKMHSDSDLESVPFSDSIFGHRGA 480

Query: 481 TLNRRMLMIEPPYKVYGEDRKQSHSTNRTSRTTGEQNGKPIARSSGDSKAAKETMSSDRK 540
           TLNRRMLMIEPPYKV+GEDRKQSHS  RTSRTTGEQNGKPIARSSGDSKAAKETMSSDRK
Sbjct: 481 TLNRRMLMIEPPYKVHGEDRKQSHS--RTSRTTGEQNGKPIARSSGDSKAAKETMSSDRK 540

Query: 541 TEVKTGGDADTKKHFKVSMSASEDKSSNELKHKPSSKSAASTSGMPTFDAKPTKSDADNS 600
           TEVKTGGDADTKKH KVSMS SEDKSSNELKHKPSSKSAASTS MPT DAKPTKSDADNS
Sbjct: 541 TEVKTGGDADTKKHSKVSMSVSEDKSSNELKHKPSSKSAASTSDMPTSDAKPTKSDADNS 600

Query: 601 LEDSSPKQSEDSMGSNTQNFKPSLPAVSSPEDVKKPGGTTSAAASQPRIEGEQTTVSNTT 660
           LEDSSPKQSEDS GSN QNFKPSLPAVSSPEDVKKPGGTTSAAASQ RI GEQ     TT
Sbjct: 601 LEDSSPKQSEDSFGSNMQNFKPSLPAVSSPEDVKKPGGTTSAAASQQRIVGEQ-----TT 660

Query: 661 VSNPSAKKPGVEENIILGVALDGSKRTLPIEDNVPTTASGAKDLAAFNGATMADKNTKRQ 720
           V NPS KKPGVEENIILGVALDG KRTLPIEDN+PT ASGAKDLA FNGATMADKNTK Q
Sbjct: 661 VPNPSTKKPGVEENIILGVALDGPKRTLPIEDNLPTAASGAKDLAVFNGATMADKNTKCQ 720

Query: 721 SPSSPTTSSSE 731
           SPSSPTTSSSE
Sbjct: 721 SPSSPTTSSSE 724

BLAST of PI0002717 vs. NCBI nr
Match: XP_011650599.1 (mechanosensitive ion channel protein 2, chloroplastic isoform X2 [Cucumis sativus] >XP_031739046.1 mechanosensitive ion channel protein 2, chloroplastic isoform X2 [Cucumis sativus] >XP_031739047.1 mechanosensitive ion channel protein 2, chloroplastic isoform X2 [Cucumis sativus] >KGN56304.1 hypothetical protein Csa_009698 [Cucumis sativus])

HSP 1 Score: 1314.7 bits (3401), Expect = 0.0e+00
Identity = 687/731 (93.98%), Postives = 702/731 (96.03%), Query Frame = 0

Query: 1   MVLVGSLQLSYHLGPWRNYLHEKNLKFTSQSYGIRLLNVASSSSLLFQ-DTWSTHLFSMK 60
           MVLVGSLQLSYHL PWRNYLHE+NLKFTSQ YGIRLLNV+SSSSLLFQ DTWSTHLFSMK
Sbjct: 1   MVLVGSLQLSYHLRPWRNYLHEENLKFTSQRYGIRLLNVSSSSSLLFQKDTWSTHLFSMK 60

Query: 61  YPPNYTVPSRYNVFRCRSSLLTNQPLDPPGMKAAIVTLTRFCNVLGGCPPPVIKLVPAVC 120
           YPPNYTVPSRYNVFRC+SSL+TNQPLDPPGMKAAIVTL RFCNVLGGCPPPVIKL+P VC
Sbjct: 61  YPPNYTVPSRYNVFRCQSSLMTNQPLDPPGMKAAIVTLMRFCNVLGGCPPPVIKLLPPVC 120

Query: 121 IIIFAVWGLGPFLRYTRSLFHNDSNWKKSRTYNVMTLYLQPLLLWTGATLICRALDPVVL 180
           II+FAVWGLGPFL YTRSLFHNDSNWKKSRTYNVMTL+LQPLLLWTGATLICRALDP+VL
Sbjct: 121 IILFAVWGLGPFLCYTRSLFHNDSNWKKSRTYNVMTLFLQPLLLWTGATLICRALDPIVL 180

Query: 181 RTESSQVVKQRVLNFVRSLSTVLASAYCLSSMIQQAQKFFSESTESSDARNMGFQFAWKA 240
           RTESSQVVKQRVLNFVRSLSTVLASAYCLSSMIQQAQKFFSESTESSDARNMGFQFAWKA
Sbjct: 181 RTESSQVVKQRVLNFVRSLSTVLASAYCLSSMIQQAQKFFSESTESSDARNMGFQFAWKA 240

Query: 241 VYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 300
           VYSAVWVAA+SLFMELLGFSTQK LTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV
Sbjct: 241 VYSAVWVAAISLFMELLGFSTQKLLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 300

Query: 301 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTMNVVRNLSQKTHWRIK 360
           NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTMNVVRNLSQKTHWRIK
Sbjct: 301 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTMNVVRNLSQKTHWRIK 360

Query: 361 THIAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVDPENQALLILISCFVK 420
           TH+AISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVD ENQALLILISCFVK
Sbjct: 361 THLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVDSENQALLILISCFVK 420

Query: 421 TSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGQRGA 480
           TSHFEEYLCVKEAIILDLLRVIRHH ARLATPIRTMQKMHSDSDLE+VPFSDSIFG RGA
Sbjct: 421 TSHFEEYLCVKEAIILDLLRVIRHHGARLATPIRTMQKMHSDSDLENVPFSDSIFGHRGA 480

Query: 481 TLNRRMLMIEPPYKVYGEDRKQSHSTNRTSRTTGEQNGKPIARSSGDSKAAKETMSSDRK 540
           TLNRRMLMIEPPYKVYGEDRKQSHS  RTSRTTGEQNGKPIARSSGDSKAAKETMSSDRK
Sbjct: 481 TLNRRMLMIEPPYKVYGEDRKQSHS--RTSRTTGEQNGKPIARSSGDSKAAKETMSSDRK 540

Query: 541 TEVKTGGDADTKKHFKVSMSASEDKSSNELKHKPSSKSAASTSGMPTFDAKPTKSDADNS 600
            EVKTGGD DTKKH K SMSASEDKSSNELK+KPSSKSAASTS  PT +AKPTKSDADNS
Sbjct: 541 IEVKTGGDGDTKKHSKASMSASEDKSSNELKYKPSSKSAASTSDTPTSNAKPTKSDADNS 600

Query: 601 LEDSSPKQSEDSMGSNTQNFKPSLPAVSSPEDVKKPGGTTSAAASQPRIEGEQTTVSNTT 660
           LEDS PKQS+DS+GSN QNFKPSLPAVSSPEDVKKPGGTTSAAASQPRIEGEQ     TT
Sbjct: 601 LEDSRPKQSKDSLGSNMQNFKPSLPAVSSPEDVKKPGGTTSAAASQPRIEGEQ-----TT 660

Query: 661 VSNPSAKKPGVEENIILGVALDGSKRTLPIEDNVPTTASGAKDLAAFNGATMADKNTKRQ 720
           VSNPS KKPGVEENIILGVALDGSKRTLPIEDNVPTTASGAKDLAAFNGAT  DKNTKRQ
Sbjct: 661 VSNPSTKKPGVEENIILGVALDGSKRTLPIEDNVPTTASGAKDLAAFNGATTTDKNTKRQ 720

Query: 721 SPSSPTTSSSE 731
           SPSSPTTSSSE
Sbjct: 721 SPSSPTTSSSE 724

BLAST of PI0002717 vs. NCBI nr
Match: XP_031739045.1 (mechanosensitive ion channel protein 2, chloroplastic isoform X1 [Cucumis sativus])

HSP 1 Score: 1314.7 bits (3401), Expect = 0.0e+00
Identity = 687/731 (93.98%), Postives = 702/731 (96.03%), Query Frame = 0

Query: 1   MVLVGSLQLSYHLGPWRNYLHEKNLKFTSQSYGIRLLNVASSSSLLFQ-DTWSTHLFSMK 60
           MVLVGSLQLSYHL PWRNYLHE+NLKFTSQ YGIRLLNV+SSSSLLFQ DTWSTHLFSMK
Sbjct: 57  MVLVGSLQLSYHLRPWRNYLHEENLKFTSQRYGIRLLNVSSSSSLLFQKDTWSTHLFSMK 116

Query: 61  YPPNYTVPSRYNVFRCRSSLLTNQPLDPPGMKAAIVTLTRFCNVLGGCPPPVIKLVPAVC 120
           YPPNYTVPSRYNVFRC+SSL+TNQPLDPPGMKAAIVTL RFCNVLGGCPPPVIKL+P VC
Sbjct: 117 YPPNYTVPSRYNVFRCQSSLMTNQPLDPPGMKAAIVTLMRFCNVLGGCPPPVIKLLPPVC 176

Query: 121 IIIFAVWGLGPFLRYTRSLFHNDSNWKKSRTYNVMTLYLQPLLLWTGATLICRALDPVVL 180
           II+FAVWGLGPFL YTRSLFHNDSNWKKSRTYNVMTL+LQPLLLWTGATLICRALDP+VL
Sbjct: 177 IILFAVWGLGPFLCYTRSLFHNDSNWKKSRTYNVMTLFLQPLLLWTGATLICRALDPIVL 236

Query: 181 RTESSQVVKQRVLNFVRSLSTVLASAYCLSSMIQQAQKFFSESTESSDARNMGFQFAWKA 240
           RTESSQVVKQRVLNFVRSLSTVLASAYCLSSMIQQAQKFFSESTESSDARNMGFQFAWKA
Sbjct: 237 RTESSQVVKQRVLNFVRSLSTVLASAYCLSSMIQQAQKFFSESTESSDARNMGFQFAWKA 296

Query: 241 VYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 300
           VYSAVWVAA+SLFMELLGFSTQK LTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV
Sbjct: 297 VYSAVWVAAISLFMELLGFSTQKLLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 356

Query: 301 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTMNVVRNLSQKTHWRIK 360
           NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTMNVVRNLSQKTHWRIK
Sbjct: 357 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTMNVVRNLSQKTHWRIK 416

Query: 361 THIAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVDPENQALLILISCFVK 420
           TH+AISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVD ENQALLILISCFVK
Sbjct: 417 THLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVDSENQALLILISCFVK 476

Query: 421 TSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGQRGA 480
           TSHFEEYLCVKEAIILDLLRVIRHH ARLATPIRTMQKMHSDSDLE+VPFSDSIFG RGA
Sbjct: 477 TSHFEEYLCVKEAIILDLLRVIRHHGARLATPIRTMQKMHSDSDLENVPFSDSIFGHRGA 536

Query: 481 TLNRRMLMIEPPYKVYGEDRKQSHSTNRTSRTTGEQNGKPIARSSGDSKAAKETMSSDRK 540
           TLNRRMLMIEPPYKVYGEDRKQSHS  RTSRTTGEQNGKPIARSSGDSKAAKETMSSDRK
Sbjct: 537 TLNRRMLMIEPPYKVYGEDRKQSHS--RTSRTTGEQNGKPIARSSGDSKAAKETMSSDRK 596

Query: 541 TEVKTGGDADTKKHFKVSMSASEDKSSNELKHKPSSKSAASTSGMPTFDAKPTKSDADNS 600
            EVKTGGD DTKKH K SMSASEDKSSNELK+KPSSKSAASTS  PT +AKPTKSDADNS
Sbjct: 597 IEVKTGGDGDTKKHSKASMSASEDKSSNELKYKPSSKSAASTSDTPTSNAKPTKSDADNS 656

Query: 601 LEDSSPKQSEDSMGSNTQNFKPSLPAVSSPEDVKKPGGTTSAAASQPRIEGEQTTVSNTT 660
           LEDS PKQS+DS+GSN QNFKPSLPAVSSPEDVKKPGGTTSAAASQPRIEGEQ     TT
Sbjct: 657 LEDSRPKQSKDSLGSNMQNFKPSLPAVSSPEDVKKPGGTTSAAASQPRIEGEQ-----TT 716

Query: 661 VSNPSAKKPGVEENIILGVALDGSKRTLPIEDNVPTTASGAKDLAAFNGATMADKNTKRQ 720
           VSNPS KKPGVEENIILGVALDGSKRTLPIEDNVPTTASGAKDLAAFNGAT  DKNTKRQ
Sbjct: 717 VSNPSTKKPGVEENIILGVALDGSKRTLPIEDNVPTTASGAKDLAAFNGATTTDKNTKRQ 776

Query: 721 SPSSPTTSSSE 731
           SPSSPTTSSSE
Sbjct: 777 SPSSPTTSSSE 780

BLAST of PI0002717 vs. NCBI nr
Match: KAA0060448.1 (mechanosensitive ion channel protein 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1307.4 bits (3382), Expect = 0.0e+00
Identity = 692/748 (92.51%), Postives = 702/748 (93.85%), Query Frame = 0

Query: 1   MVLVGSLQLSYHLGPWRNYLHEKNLKFTSQSYGIRLLNVASSSSLLFQ-DTWSTHLFSMK 60
           MVLVGSLQLSYHLGPWRNYLHE+NLKFTSQSYGIRLLNVASSSSLLFQ DTWSTHLFSMK
Sbjct: 1   MVLVGSLQLSYHLGPWRNYLHEENLKFTSQSYGIRLLNVASSSSLLFQKDTWSTHLFSMK 60

Query: 61  YPPNYTVPSRYNVFRCRSSLLTNQPLDPPGMKAAIVTLTRFCNVLGGCPPPVIKLVPAVC 120
           YPPNYTVPSRYNVFRC+SSL+TNQPLD PGMKA+IVTLTRFCNVLGGCPP VIKLVP VC
Sbjct: 61  YPPNYTVPSRYNVFRCQSSLMTNQPLDLPGMKASIVTLTRFCNVLGGCPPSVIKLVPPVC 120

Query: 121 IIIFAVWGLGPFLRYTRSLFHNDSNWKKSRTYNVMTLYLQPLLLWTGATLICRALDPVVL 180
           IIIFAVWGLGPFLR+TRSLFHNDSNWKKSRTYNVMTLYLQPLLLWTGATLICRALDPVVL
Sbjct: 121 IIIFAVWGLGPFLRHTRSLFHNDSNWKKSRTYNVMTLYLQPLLLWTGATLICRALDPVVL 180

Query: 181 RTESSQVVKQRVLNFVRSLSTVLASAYCLSSMIQQAQKFFSESTESSDARNMGFQFAWKA 240
           RTESSQVVKQRVLNFVRSLSTVLASAYCLSSMIQQAQKFFSE+TESSDARNMGFQFAWKA
Sbjct: 181 RTESSQVVKQRVLNFVRSLSTVLASAYCLSSMIQQAQKFFSENTESSDARNMGFQFAWKA 240

Query: 241 VYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 300
           VYSAVWVAA+SLFMELLGFSTQK LTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV
Sbjct: 241 VYSAVWVAAISLFMELLGFSTQKLLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 300

Query: 301 NEWIQTKIEGYEVSGTVE-----------------HVGWWSPTIIRGEDREAVHIPNHQF 360
           NEWIQTKIEGYEVSGTVE                 HVGWWSPTIIRGEDREAVHIPNHQF
Sbjct: 301 NEWIQTKIEGYEVSGTVEVCMFLLNMYHKLLMSKKHVGWWSPTIIRGEDREAVHIPNHQF 360

Query: 361 TMNVVRNLSQKTHWRIKTHIAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLEN 420
           TMNVVRNLSQKTHWRIKTH+AISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLEN
Sbjct: 361 TMNVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLEN 420

Query: 421 VDPENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDS 480
           VD ENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHH ARLATPIRTMQKMHSDS
Sbjct: 421 VDSENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHHGARLATPIRTMQKMHSDS 480

Query: 481 DLESVPFSDSIFGQRGATLNRRMLMIEPPYKVYGEDRKQSHSTNRTSRTTGEQNGKPIAR 540
           DLESVPFSDSIFG RGATLNRRMLMIEPPYKV+GEDRKQSHS  RTSRTTGEQNGKPIAR
Sbjct: 481 DLESVPFSDSIFGHRGATLNRRMLMIEPPYKVHGEDRKQSHS--RTSRTTGEQNGKPIAR 540

Query: 541 SSGDSKAAKETMSSDRKTEVKTGGDADTKKHFKVSMSASEDKSSNELKHKPSSKSAASTS 600
           SSGDSKAAKETMSSDRKTEVKTGGDADTKKH KVSMS SEDKSSNELKHKPSSKSAASTS
Sbjct: 541 SSGDSKAAKETMSSDRKTEVKTGGDADTKKHSKVSMSVSEDKSSNELKHKPSSKSAASTS 600

Query: 601 GMPTFDAKPTKSDADNSLEDSSPKQSEDSMGSNTQNFKPSLPAVSSPEDVKKPGGTTSAA 660
            MPT DAKPTKSDADNSLEDSSPKQSEDS GSN QNFKPSLP VSSPEDVKKPGGTTSAA
Sbjct: 601 DMPTSDAKPTKSDADNSLEDSSPKQSEDSFGSNMQNFKPSLPTVSSPEDVKKPGGTTSAA 660

Query: 661 ASQPRIEGEQTTVSNTTVSNPSAKKPGVEENIILGVALDGSKRTLPIEDNVPTTASGAKD 720
           ASQ RI GEQ     TTVSNPS KKPGVEENIILGVALDG KRTLPIEDN+PT ASGAKD
Sbjct: 661 ASQQRIVGEQ-----TTVSNPSTKKPGVEENIILGVALDGPKRTLPIEDNLPTAASGAKD 720

Query: 721 LAAFNGATMADKNTKRQSPSSPTTSSSE 731
           LA FNGATMADKNTK QSPSSPTTSSSE
Sbjct: 721 LAVFNGATMADKNTKCQSPSSPTTSSSE 741

BLAST of PI0002717 vs. NCBI nr
Match: XP_008452168.1 (PREDICTED: mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucumis melo] >XP_008452169.1 PREDICTED: mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucumis melo] >XP_008452170.1 PREDICTED: mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucumis melo] >XP_008452171.1 PREDICTED: mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 [Cucumis melo])

HSP 1 Score: 1307.0 bits (3381), Expect = 0.0e+00
Identity = 692/748 (92.51%), Postives = 702/748 (93.85%), Query Frame = 0

Query: 1   MVLVGSLQLSYHLGPWRNYLHEKNLKFTSQSYGIRLLNVASSSSLLFQ-DTWSTHLFSMK 60
           MVLVGSLQLSYHLGPWRNYLHE+NLKFTSQSYGIRLLNVASSSSLLFQ DTWSTHLFSMK
Sbjct: 1   MVLVGSLQLSYHLGPWRNYLHEENLKFTSQSYGIRLLNVASSSSLLFQKDTWSTHLFSMK 60

Query: 61  YPPNYTVPSRYNVFRCRSSLLTNQPLDPPGMKAAIVTLTRFCNVLGGCPPPVIKLVPAVC 120
           YPPNYTVPSRYNVFRC+SSL+TNQPLD PGMKA+IVTLTRFCNVLGGCPP VIKLVP VC
Sbjct: 61  YPPNYTVPSRYNVFRCQSSLMTNQPLDLPGMKASIVTLTRFCNVLGGCPPSVIKLVPPVC 120

Query: 121 IIIFAVWGLGPFLRYTRSLFHNDSNWKKSRTYNVMTLYLQPLLLWTGATLICRALDPVVL 180
           IIIFAVWGLGPFLR+TRSLFHNDSNWKKSRTYNVMTLYLQPLLLWTGATLICRALDPVVL
Sbjct: 121 IIIFAVWGLGPFLRHTRSLFHNDSNWKKSRTYNVMTLYLQPLLLWTGATLICRALDPVVL 180

Query: 181 RTESSQVVKQRVLNFVRSLSTVLASAYCLSSMIQQAQKFFSESTESSDARNMGFQFAWKA 240
           RTESSQVVKQRVLNFVRSLSTVLASAYCLSSMIQQAQKFFSE+TESSDARNMGFQFAWKA
Sbjct: 181 RTESSQVVKQRVLNFVRSLSTVLASAYCLSSMIQQAQKFFSENTESSDARNMGFQFAWKA 240

Query: 241 VYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 300
           VYSAVWVAA+SLFMELLGFSTQK LTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV
Sbjct: 241 VYSAVWVAAISLFMELLGFSTQKLLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 300

Query: 301 NEWIQTKIEGYEVSGTVE-----------------HVGWWSPTIIRGEDREAVHIPNHQF 360
           NEWIQTKIEGYEVSGTVE                 HVGWWSPTIIRGEDREAVHIPNHQF
Sbjct: 301 NEWIQTKIEGYEVSGTVEVCMFLLNMYHKLLMSKKHVGWWSPTIIRGEDREAVHIPNHQF 360

Query: 361 TMNVVRNLSQKTHWRIKTHIAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLEN 420
           TMNVVRNLSQKTHWRIKTH+AISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLEN
Sbjct: 361 TMNVVRNLSQKTHWRIKTHLAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLEN 420

Query: 421 VDPENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDS 480
           VD ENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHH ARLATPIRTMQKMHSDS
Sbjct: 421 VDSENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHHGARLATPIRTMQKMHSDS 480

Query: 481 DLESVPFSDSIFGQRGATLNRRMLMIEPPYKVYGEDRKQSHSTNRTSRTTGEQNGKPIAR 540
           DLESVPFSDSIFG RGATLNRRMLMIEPPYKV+GEDRKQSHS  RTSRTTGEQNGKPIAR
Sbjct: 481 DLESVPFSDSIFGHRGATLNRRMLMIEPPYKVHGEDRKQSHS--RTSRTTGEQNGKPIAR 540

Query: 541 SSGDSKAAKETMSSDRKTEVKTGGDADTKKHFKVSMSASEDKSSNELKHKPSSKSAASTS 600
           SSGDSKAAKETMSSDRKTEVKTGGDADTKKH KVSMS SEDKSSNELKHKPSSKSAASTS
Sbjct: 541 SSGDSKAAKETMSSDRKTEVKTGGDADTKKHSKVSMSVSEDKSSNELKHKPSSKSAASTS 600

Query: 601 GMPTFDAKPTKSDADNSLEDSSPKQSEDSMGSNTQNFKPSLPAVSSPEDVKKPGGTTSAA 660
            MPT DAKPTKSDADNSLEDSSPKQSEDS GSN QNFKPSLPAVSSPEDVKKPGGTTSAA
Sbjct: 601 DMPTSDAKPTKSDADNSLEDSSPKQSEDSFGSNMQNFKPSLPAVSSPEDVKKPGGTTSAA 660

Query: 661 ASQPRIEGEQTTVSNTTVSNPSAKKPGVEENIILGVALDGSKRTLPIEDNVPTTASGAKD 720
           ASQ RI GEQ     TTV NPS KKPGVEENIILGVALDG KRTLPIEDN+PT ASGAKD
Sbjct: 661 ASQQRIVGEQ-----TTVPNPSTKKPGVEENIILGVALDGPKRTLPIEDNLPTAASGAKD 720

Query: 721 LAAFNGATMADKNTKRQSPSSPTTSSSE 731
           LA FNGATMADKNTK QSPSSPTTSSSE
Sbjct: 721 LAVFNGATMADKNTKCQSPSSPTTSSSE 741

BLAST of PI0002717 vs. TAIR 10
Match: AT5G10490.1 (MSCS-like 2 )

HSP 1 Score: 674.5 bits (1739), Expect = 9.7e-194
Identity = 413/745 (55.44%), Postives = 496/745 (66.58%), Query Frame = 0

Query: 1   MVLVGSLQLSYHLGPWRNYLHEKNLKFTS-QSYGIRLLNVASSSSLLFQDTWSTHLFSMK 60
           M L G+LQLS+ LG  RN       +F + ++  +R     S+  L        H FS  
Sbjct: 1   MALYGTLQLSHSLGLCRNQ------RFCNPENSAMRRRLHISNGPLSLGVPLGQHGFSNI 60

Query: 61  YPPNY------TVPSRYNVFRCRSSLLTNQPLDPPGMKAAIVTLTRFCNVLGGCPPPVIK 120
              NY      +VP R   FRC S   + + ++ P +KA  V LT+   ++    P V K
Sbjct: 61  LLSNYLRRPICSVPCRTTAFRCHSFSASGKAIE-PAVKAVTVVLTKSHGLMQQF-PFVYK 120

Query: 121 LVPAVCIIIFAVWGLGPFLRYTRSLF--HNDSNWKKSRTYNVMTLYLQPLLLWTGATLIC 180
           LVPAV +++F++WGL PF R  R++    ND+ WKKS TY+VMT Y+QPLLLW GA  IC
Sbjct: 121 LVPAVALLVFSLWGLVPFARQGRNILLNKNDNGWKKSGTYHVMTSYVQPLLLWLGALFIC 180

Query: 181 RALDPVVLRTESSQVVKQRVLNFVRSLSTVLASAYCLSSMIQQAQKFFSESTESSDARNM 240
           RALDPVVL TE+S++VK R+LNFVRSLSTVLA AYCLSS+IQQ QK FSE++  SD RNM
Sbjct: 181 RALDPVVLPTEASKIVKDRLLNFVRSLSTVLAFAYCLSSLIQQTQKLFSETSNPSDTRNM 240

Query: 241 GFQFAWKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMI 300
           GFQFA KA+YSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVL+TLAGREI TNFLSSVMI
Sbjct: 241 GFQFAGKALYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLITLAGREILTNFLSSVMI 300

Query: 301 HATRPFVVNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTMNVVRNLS 360
           HATRPFV+NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREA+HIPNH+FT+NVVRNL+
Sbjct: 301 HATRPFVLNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAIHIPNHKFTVNVVRNLT 360

Query: 361 QKTHWRIKTHIAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVDPENQALL 420
           QKTHWRIKTH+AISHLDVNKINNIVADMRKVLAKNP VEQQRLHRRVFLENV PENQAL 
Sbjct: 361 QKTHWRIKTHLAISHLDVNKINNIVADMRKVLAKNPMVEQQRLHRRVFLENVIPENQALS 420

Query: 421 ILISCFVKTSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDLESVPFSD 480
           ILISCFVKTSH EEYL VKEAI+LDLLRVI HHRARLATPIRT++KM++++D+E+ PF +
Sbjct: 421 ILISCFVKTSHHEEYLGVKEAILLDLLRVISHHRARLATPIRTIRKMYTETDVENTPFGE 480

Query: 481 SIFGQRGATLNRRMLMIEPPYKVYGEDRKQSHSTNRTSRTTGEQNGKPIARSSGDSKAAK 540
           S++G  G T  R +++IEP YK+ GED+  S S NR ++ T EQ  K      G +  +K
Sbjct: 481 SMYG--GVTSRRPLMLIEPAYKINGEDK--SKSQNRAAKPTAEQENK------GSNPKSK 540

Query: 541 ETMSSDRKTEVKTGGDADTKKHFKVSMSASEDKSSNELKHKPSSKSAASTSGMPTFDAKP 600
           ET S D K  VK G           S  +  +K   E   KP  K+              
Sbjct: 541 ETSSPDLKANVKVG----------ESPVSDTNKVPEETVAKPVIKAV------------- 600

Query: 601 TKSDADNSLEDSSPKQSEDSMGSNTQNFKPSLPAVSSPEDVKKPGGTTSAAASQPRIEGE 660
                      S P   +D+  S T+  KP           K+ GGT            +
Sbjct: 601 -----------SKPPTPKDTETSGTE--KPK---------AKRSGGTI-----------K 660

Query: 661 QTTVSNTTVSNPSAKKPGVEENIILGVALDGSKRTLPIEDNV--PTTASGAKDL-----A 720
            T    T  S  SA +  +EENI+LGVAL+GSKRTLPIE+ +  P   + AK+L     +
Sbjct: 661 STKTDETDSSTSSASRSTLEENIVLGVALEGSKRTLPIEEEIHSPPMETDAKELTGARRS 670

Query: 721 AFNGATMADKNTKRQSPSSPTTSSS 730
             NG  +ADK  K  S S P + +S
Sbjct: 721 GGNGPLVADKEQK-DSQSQPNSGAS 670

BLAST of PI0002717 vs. TAIR 10
Match: AT5G10490.2 (MSCS-like 2 )

HSP 1 Score: 668.3 bits (1723), Expect = 7.0e-192
Identity = 394/677 (58.20%), Postives = 469/677 (69.28%), Query Frame = 0

Query: 62  PNYTVPSRYNVFRCRSSLLTNQPLDPPGMKAAIVTLTRFCNVLGGCPPPVIKLVPAVCII 121
           P  +VP R   FRC S   + + ++ P +KA  V LT+   ++    P V KLVPAV ++
Sbjct: 35  PICSVPCRTTAFRCHSFSASGKAIE-PAVKAVTVVLTKSHGLMQQF-PFVYKLVPAVALL 94

Query: 122 IFAVWGLGPFLRYTRSLF--HNDSNWKKSRTYNVMTLYLQPLLLWTGATLICRALDPVVL 181
           +F++WGL PF R  R++    ND+ WKKS TY+VMT Y+QPLLLW GA  ICRALDPVVL
Sbjct: 95  VFSLWGLVPFARQGRNILLNKNDNGWKKSGTYHVMTSYVQPLLLWLGALFICRALDPVVL 154

Query: 182 RTESSQVVKQRVLNFVRSLSTVLASAYCLSSMIQQAQKFFSESTESSDARNMGFQFAWKA 241
            TE+S++VK R+LNFVRSLSTVLA AYCLSS+IQQ QK FSE++  SD RNMGFQFA KA
Sbjct: 155 PTEASKIVKDRLLNFVRSLSTVLAFAYCLSSLIQQTQKLFSETSNPSDTRNMGFQFAGKA 214

Query: 242 VYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 301
           +YSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVL+TLAGREI TNFLSSVMIHATRPFV+
Sbjct: 215 LYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLITLAGREILTNFLSSVMIHATRPFVL 274

Query: 302 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTMNVVRNLSQKTHWRIK 361
           NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREA+HIPNH+FT+NVVRNL+QKTHWRIK
Sbjct: 275 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAIHIPNHKFTVNVVRNLTQKTHWRIK 334

Query: 362 THIAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVDPENQALLILISCFVK 421
           TH+AISHLDVNKINNIVADMRKVLAKNP VEQQRLHRRVFLENV PENQAL ILISCFVK
Sbjct: 335 THLAISHLDVNKINNIVADMRKVLAKNPMVEQQRLHRRVFLENVIPENQALSILISCFVK 394

Query: 422 TSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGQRGA 481
           TSH EEYL VKEAI+LDLLRVI HHRARLATPIRT++KM++++D+E+ PF +S++G  G 
Sbjct: 395 TSHHEEYLGVKEAILLDLLRVISHHRARLATPIRTIRKMYTETDVENTPFGESMYG--GV 454

Query: 482 TLNRRMLMIEPPYKVYGEDRKQSHSTNRTSRTTGEQNGKPIARSSGDSKAAKETMSSDRK 541
           T  R +++IEP YK+ GED+  S S NR ++ T EQ  K      G +  +KET S D K
Sbjct: 455 TSRRPLMLIEPAYKINGEDK--SKSQNRAAKPTAEQENK------GSNPKSKETSSPDLK 514

Query: 542 TEVKTGGDADTKKHFKVSMSASEDKSSNELKHKPSSKSAASTSGMPTFDAKPTKSDADNS 601
             VK G           S  +  +K   E   KP  K+                      
Sbjct: 515 ANVKVG----------ESPVSDTNKVPEETVAKPVIKAV--------------------- 574

Query: 602 LEDSSPKQSEDSMGSNTQNFKPSLPAVSSPEDVKKPGGTTSAAASQPRIEGEQTTVSNTT 661
              S P   +D+  S T+  KP           K+ GGT            + T    T 
Sbjct: 575 ---SKPPTPKDTETSGTE--KPK---------AKRSGGTI-----------KSTKTDETD 634

Query: 662 VSNPSAKKPGVEENIILGVALDGSKRTLPIEDNV--PTTASGAKDL-----AAFNGATMA 721
            S  SA +  +EENI+LGVAL+GSKRTLPIE+ +  P   + AK+L     +  NG  +A
Sbjct: 635 SSTSSASRSTLEENIVLGVALEGSKRTLPIEEEIHSPPMETDAKELTGARRSGGNGPLVA 642

Query: 722 DKNTKRQSPSSPTTSSS 730
           DK  K  S S P + +S
Sbjct: 695 DKEQK-DSQSQPNSGAS 642

BLAST of PI0002717 vs. TAIR 10
Match: AT5G10490.3 (MSCS-like 2 )

HSP 1 Score: 668.3 bits (1723), Expect = 7.0e-192
Identity = 394/677 (58.20%), Postives = 469/677 (69.28%), Query Frame = 0

Query: 62  PNYTVPSRYNVFRCRSSLLTNQPLDPPGMKAAIVTLTRFCNVLGGCPPPVIKLVPAVCII 121
           P  +VP R   FRC S   + + ++ P +KA  V LT+   ++    P V KLVPAV ++
Sbjct: 61  PICSVPCRTTAFRCHSFSASGKAIE-PAVKAVTVVLTKSHGLMQQF-PFVYKLVPAVALL 120

Query: 122 IFAVWGLGPFLRYTRSLF--HNDSNWKKSRTYNVMTLYLQPLLLWTGATLICRALDPVVL 181
           +F++WGL PF R  R++    ND+ WKKS TY+VMT Y+QPLLLW GA  ICRALDPVVL
Sbjct: 121 VFSLWGLVPFARQGRNILLNKNDNGWKKSGTYHVMTSYVQPLLLWLGALFICRALDPVVL 180

Query: 182 RTESSQVVKQRVLNFVRSLSTVLASAYCLSSMIQQAQKFFSESTESSDARNMGFQFAWKA 241
            TE+S++VK R+LNFVRSLSTVLA AYCLSS+IQQ QK FSE++  SD RNMGFQFA KA
Sbjct: 181 PTEASKIVKDRLLNFVRSLSTVLAFAYCLSSLIQQTQKLFSETSNPSDTRNMGFQFAGKA 240

Query: 242 VYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLLTLAGREIFTNFLSSVMIHATRPFVV 301
           +YSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVL+TLAGREI TNFLSSVMIHATRPFV+
Sbjct: 241 LYSAVWVAAVSLFMELLGFSTQKWLTAGGLGTVLITLAGREILTNFLSSVMIHATRPFVL 300

Query: 302 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAVHIPNHQFTMNVVRNLSQKTHWRIK 361
           NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREA+HIPNH+FT+NVVRNL+QKTHWRIK
Sbjct: 301 NEWIQTKIEGYEVSGTVEHVGWWSPTIIRGEDREAIHIPNHKFTVNVVRNLTQKTHWRIK 360

Query: 362 THIAISHLDVNKINNIVADMRKVLAKNPQVEQQRLHRRVFLENVDPENQALLILISCFVK 421
           TH+AISHLDVNKINNIVADMRKVLAKNP VEQQRLHRRVFLENV PENQAL ILISCFVK
Sbjct: 361 THLAISHLDVNKINNIVADMRKVLAKNPMVEQQRLHRRVFLENVIPENQALSILISCFVK 420

Query: 422 TSHFEEYLCVKEAIILDLLRVIRHHRARLATPIRTMQKMHSDSDLESVPFSDSIFGQRGA 481
           TSH EEYL VKEAI+LDLLRVI HHRARLATPIRT++KM++++D+E+ PF +S++G  G 
Sbjct: 421 TSHHEEYLGVKEAILLDLLRVISHHRARLATPIRTIRKMYTETDVENTPFGESMYG--GV 480

Query: 482 TLNRRMLMIEPPYKVYGEDRKQSHSTNRTSRTTGEQNGKPIARSSGDSKAAKETMSSDRK 541
           T  R +++IEP YK+ GED+  S S NR ++ T EQ  K      G +  +KET S D K
Sbjct: 481 TSRRPLMLIEPAYKINGEDK--SKSQNRAAKPTAEQENK------GSNPKSKETSSPDLK 540

Query: 542 TEVKTGGDADTKKHFKVSMSASEDKSSNELKHKPSSKSAASTSGMPTFDAKPTKSDADNS 601
             VK G           S  +  +K   E   KP  K+                      
Sbjct: 541 ANVKVG----------ESPVSDTNKVPEETVAKPVIKAV--------------------- 600

Query: 602 LEDSSPKQSEDSMGSNTQNFKPSLPAVSSPEDVKKPGGTTSAAASQPRIEGEQTTVSNTT 661
              S P   +D+  S T+  KP           K+ GGT            + T    T 
Sbjct: 601 ---SKPPTPKDTETSGTE--KPK---------AKRSGGTI-----------KSTKTDETD 660

Query: 662 VSNPSAKKPGVEENIILGVALDGSKRTLPIEDNV--PTTASGAKDL-----AAFNGATMA 721
            S  SA +  +EENI+LGVAL+GSKRTLPIE+ +  P   + AK+L     +  NG  +A
Sbjct: 661 SSTSSASRSTLEENIVLGVALEGSKRTLPIEEEIHSPPMETDAKELTGARRSGGNGPLVA 668

Query: 722 DKNTKRQSPSSPTTSSS 730
           DK  K  S S P + +S
Sbjct: 721 DKEQK-DSQSQPNSGAS 668

BLAST of PI0002717 vs. TAIR 10
Match: AT1G58200.1 (MSCS-like 3 )

HSP 1 Score: 611.3 bits (1575), Expect = 1.0e-174
Identity = 358/670 (53.43%), Postives = 447/670 (66.72%), Query Frame = 0

Query: 34  IRLLNVASSSSLLFQDTWSTHLFSMKYPPNYTVPSRYNVFRCRSSLLTNQPLDPPGMKAA 93
           + L     SS    QD WS  L          V SR N F CRS+L      + P +K+ 
Sbjct: 35  VYLTRTGLSSCATRQDVWSLQLLESLSGSIVPVSSRCNAFVCRSALSPGNGNEGPILKST 94

Query: 94  IVTLTRFCNVLGGCPPPVIKLVPAVCIIIFAVWGLGPFLRYTR-SLFH--NDSNWKKSRT 153
            V  TR  + LGG  P ++KL+PAV I+ FA WGL P LR  R +LF   ND+N +KS T
Sbjct: 95  AVIFTRVYDALGG-NPHLVKLIPAVGILAFATWGLRPLLRLARTTLFEKGNDANSQKSST 154

Query: 154 YNVMTLYLQPLLLWTGATLICRALDPVVLRTESSQVVKQRVLNFVRSLSTVLASAYCLSS 213
             ++  YLQPLLLW+GA L+CR LDP+VL + + Q +KQR+L F RS+STVLA + CLSS
Sbjct: 155 QYIVVSYLQPLLLWSGAILLCRTLDPIVLPSSAGQAIKQRLLIFARSISTVLAFSCCLSS 214

Query: 214 MIQQAQKFFSESTESSDARNMGFQFAWKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLG 273
           ++QQ QKFF E+   +D RNMGF FA KAVY+A WVAA SLFMELLGFSTQKWLTAGGLG
Sbjct: 215 LLQQVQKFFMETNNPADTRNMGFSFAGKAVYTAAWVAAASLFMELLGFSTQKWLTAGGLG 274

Query: 274 TVLLTLAGREIFTNFLSSVMIHATRPFVVNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGE 333
           TVLLTLAGREI TNFLSS+MIHATRPFV+NEWIQTKI GYEVSGTVE VGWWSPTIIRG+
Sbjct: 275 TVLLTLAGREILTNFLSSIMIHATRPFVLNEWIQTKIGGYEVSGTVEQVGWWSPTIIRGD 334

Query: 334 DREAVHIPNHQFTMNVVRNLSQKTHWRIKTHIAISHLDVNKINNIVADMRKVLAKNPQVE 393
           DREAVHIPNHQF++N+VRNL+QKTHWRIKTH+AISHLDV+KINNIVADMRKVL+KNPQ+E
Sbjct: 335 DREAVHIPNHQFSVNIVRNLTQKTHWRIKTHLAISHLDVSKINNIVADMRKVLSKNPQIE 394

Query: 394 QQRLHRRVFLENVDPENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHHRARLAT 453
           QQ++HRRVFLE++DPENQAL ILISCFVKTS FEEYLCVKEA++LDLL VIRHH ARLAT
Sbjct: 395 QQKIHRRVFLEDIDPENQALRILISCFVKTSRFEEYLCVKEAVLLDLLTVIRHHGARLAT 454

Query: 454 PIRTMQKMHSDSDLESVPFSDSIFGQRGATLNRRMLMIEPPYKVYGEDRKQSHSTNRTSR 513
           PIRT+Q+M +++++++  FSD +F Q  A +NRR ++IEP YK+  +D  +S      S 
Sbjct: 455 PIRTVQRMRNEAEVDTAGFSDIVFNQ--AAMNRRYMLIEPSYKINSDDNSKS-----PSP 514

Query: 514 TTGEQNGKPIARSSGDSKAAKETMSSDRKTEVKTGGDADTKKHFKVSMSASEDKSSNELK 573
           + G+++  P     G     ++      +T+ +T  +         S+  S  K  N+ K
Sbjct: 515 SPGQKSPSP-----GQKSEERDLQEEPSETKAETENNG--------SVPVSNAKKENQ-K 574

Query: 574 HKPSSKSAASTSGMPTFDAKPTKSDADNSLEDSSPKQSEDSMGSNTQNFKPSLPAVSSPE 633
               S S   T G  T       S +D  +   S ++ ++S+G      K     VS  E
Sbjct: 575 AALGSNSNTGTKGSST-------STSDQPVAQKSEEKKKESVG---DPHKAEKDEVSDDE 634

Query: 634 DVKKPGGTTSAAASQPRIEGEQTTVSNTTVSNPSAKKPGVEENIILGVALDGSKRTLPIE 693
              +    + A     +  GE    S       S     +EEN++LGVALDGSKRTLPI+
Sbjct: 635 ATIEQTLKSKAKQGSEKNNGE----SKARDGGGSGTSSLLEENLVLGVALDGSKRTLPID 666

Query: 694 DNVPTTASGA 701
           +     ASGA
Sbjct: 695 EE--HKASGA 666

BLAST of PI0002717 vs. TAIR 10
Match: AT1G58200.2 (MSCS-like 3 )

HSP 1 Score: 611.3 bits (1575), Expect = 1.0e-174
Identity = 358/670 (53.43%), Postives = 447/670 (66.72%), Query Frame = 0

Query: 34  IRLLNVASSSSLLFQDTWSTHLFSMKYPPNYTVPSRYNVFRCRSSLLTNQPLDPPGMKAA 93
           + L     SS    QD WS  L          V SR N F CRS+L      + P +K+ 
Sbjct: 35  VYLTRTGLSSCATRQDVWSLQLLESLSGSIVPVSSRCNAFVCRSALSPGNGNEGPILKST 94

Query: 94  IVTLTRFCNVLGGCPPPVIKLVPAVCIIIFAVWGLGPFLRYTR-SLFH--NDSNWKKSRT 153
            V  TR  + LGG  P ++KL+PAV I+ FA WGL P LR  R +LF   ND+N +KS T
Sbjct: 95  AVIFTRVYDALGG-NPHLVKLIPAVGILAFATWGLRPLLRLARTTLFEKGNDANSQKSST 154

Query: 154 YNVMTLYLQPLLLWTGATLICRALDPVVLRTESSQVVKQRVLNFVRSLSTVLASAYCLSS 213
             ++  YLQPLLLW+GA L+CR LDP+VL + + Q +KQR+L F RS+STVLA + CLSS
Sbjct: 155 QYIVVSYLQPLLLWSGAILLCRTLDPIVLPSSAGQAIKQRLLIFARSISTVLAFSCCLSS 214

Query: 214 MIQQAQKFFSESTESSDARNMGFQFAWKAVYSAVWVAAVSLFMELLGFSTQKWLTAGGLG 273
           ++QQ QKFF E+   +D RNMGF FA KAVY+A WVAA SLFMELLGFSTQKWLTAGGLG
Sbjct: 215 LLQQVQKFFMETNNPADTRNMGFSFAGKAVYTAAWVAAASLFMELLGFSTQKWLTAGGLG 274

Query: 274 TVLLTLAGREIFTNFLSSVMIHATRPFVVNEWIQTKIEGYEVSGTVEHVGWWSPTIIRGE 333
           TVLLTLAGREI TNFLSS+MIHATRPFV+NEWIQTKI GYEVSGTVE VGWWSPTIIRG+
Sbjct: 275 TVLLTLAGREILTNFLSSIMIHATRPFVLNEWIQTKIGGYEVSGTVEQVGWWSPTIIRGD 334

Query: 334 DREAVHIPNHQFTMNVVRNLSQKTHWRIKTHIAISHLDVNKINNIVADMRKVLAKNPQVE 393
           DREAVHIPNHQF++N+VRNL+QKTHWRIKTH+AISHLDV+KINNIVADMRKVL+KNPQ+E
Sbjct: 335 DREAVHIPNHQFSVNIVRNLTQKTHWRIKTHLAISHLDVSKINNIVADMRKVLSKNPQIE 394

Query: 394 QQRLHRRVFLENVDPENQALLILISCFVKTSHFEEYLCVKEAIILDLLRVIRHHRARLAT 453
           QQ++HRRVFLE++DPENQAL ILISCFVKTS FEEYLCVKEA++LDLL VIRHH ARLAT
Sbjct: 395 QQKIHRRVFLEDIDPENQALRILISCFVKTSRFEEYLCVKEAVLLDLLTVIRHHGARLAT 454

Query: 454 PIRTMQKMHSDSDLESVPFSDSIFGQRGATLNRRMLMIEPPYKVYGEDRKQSHSTNRTSR 513
           PIRT+Q+M +++++++  FSD +F Q  A +NRR ++IEP YK+  +D  +S      S 
Sbjct: 455 PIRTVQRMRNEAEVDTAGFSDIVFNQ--AAMNRRYMLIEPSYKINSDDNSKS-----PSP 514

Query: 514 TTGEQNGKPIARSSGDSKAAKETMSSDRKTEVKTGGDADTKKHFKVSMSASEDKSSNELK 573
           + G+++  P     G     ++      +T+ +T  +         S+  S  K  N+ K
Sbjct: 515 SPGQKSPSP-----GQKSEERDLQEEPSETKAETENNG--------SVPVSNAKKENQ-K 574

Query: 574 HKPSSKSAASTSGMPTFDAKPTKSDADNSLEDSSPKQSEDSMGSNTQNFKPSLPAVSSPE 633
               S S   T G  T       S +D  +   S ++ ++S+G      K     VS  E
Sbjct: 575 AALGSNSNTGTKGSST-------STSDQPVAQKSEEKKKESVG---DPHKAEKDEVSDDE 634

Query: 634 DVKKPGGTTSAAASQPRIEGEQTTVSNTTVSNPSAKKPGVEENIILGVALDGSKRTLPIE 693
              +    + A     +  GE    S       S     +EEN++LGVALDGSKRTLPI+
Sbjct: 635 ATIEQTLKSKAKQGSEKNNGE----SKARDGGGSGTSSLLEENLVLGVALDGSKRTLPID 666

Query: 694 DNVPTTASGA 701
           +     ASGA
Sbjct: 695 EE--HKASGA 666

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q56X461.4e-19255.44Mechanosensitive ion channel protein 2, chloroplastic OS=Arabidopsis thaliana OX... [more]
Q8L7W11.4e-17353.43Mechanosensitive ion channel protein 3, chloroplastic OS=Arabidopsis thaliana OX... [more]
P0AEB61.1e-2127.05Low conductance mechanosensitive channel YnaI OS=Escherichia coli O157:H7 OX=833... [more]
P0AEB51.1e-2127.05Low conductance mechanosensitive channel YnaI OS=Escherichia coli (strain K12) O... [more]
O669942.8e-1227.08Uncharacterized MscS family protein aq_812 OS=Aquifex aeolicus (strain VF5) OX=2... [more]
Match NameE-valueIdentityDescription
A0A1S3BT670.0e+0094.66mechanosensitive ion channel protein 2, chloroplastic-like isoform X2 OS=Cucumis... [more]
A0A0A0L5I60.0e+0093.98Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G114440 PE=4 SV=1[more]
A0A5A7UYX20.0e+0092.51Mechanosensitive ion channel protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3BUC50.0e+0092.51mechanosensitive ion channel protein 2, chloroplastic-like isoform X1 OS=Cucumis... [more]
A0A5D3D4R20.0e+0092.64Mechanosensitive ion channel protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
Match NameE-valueIdentityDescription
XP_008452175.10.0e+0094.66PREDICTED: mechanosensitive ion channel protein 2, chloroplastic-like isoform X2... [more]
XP_011650599.10.0e+0093.98mechanosensitive ion channel protein 2, chloroplastic isoform X2 [Cucumis sativu... [more]
XP_031739045.10.0e+0093.98mechanosensitive ion channel protein 2, chloroplastic isoform X1 [Cucumis sativu... [more]
KAA0060448.10.0e+0092.51mechanosensitive ion channel protein 2 [Cucumis melo var. makuwa][more]
XP_008452168.10.0e+0092.51PREDICTED: mechanosensitive ion channel protein 2, chloroplastic-like isoform X1... [more]
Match NameE-valueIdentityDescription
AT5G10490.19.7e-19455.44MSCS-like 2 [more]
AT5G10490.27.0e-19258.20MSCS-like 2 [more]
AT5G10490.37.0e-19258.20MSCS-like 2 [more]
AT1G58200.11.0e-17453.43MSCS-like 3 [more]
AT1G58200.21.0e-17453.43MSCS-like 3 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023408Mechanosensitive ion channel MscS domain superfamilyGENE3D2.30.30.60coord: 297..352
e-value: 2.7E-6
score: 29.3
NoneNo IPR availableGENE3D1.10.287.1260coord: 195..295
e-value: 2.5E-7
score: 32.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 531..571
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 712..730
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 605..624
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 572..588
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 686..730
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 493..671
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 506..522
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 638..666
NoneNo IPR availablePANTHERPTHR43634:SF6MECHANOSENSITIVE ION CHANNEL PROTEIN 2, CHLOROPLASTICcoord: 1..720
IPR006685Mechanosensitive ion channel MscSPFAMPF00924MS_channelcoord: 239..445
e-value: 2.3E-30
score: 105.7
IPR045042Mechanosensitive ion channel protein YnaI-likePANTHERPTHR43634OW CONDUCTANCE MECHANOSENSITIVE CHANNELcoord: 1..720
IPR010920LSM domain superfamilySUPERFAMILY50182Sm-like ribonucleoproteinscoord: 281..351

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0002717.1PI0002717.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006811 ion transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane