PI0002455 (gene) Melon (PI 482460) v1

Overview
NamePI0002455
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptionisoflavone reductase-like protein
Locationchr01: 5955924 .. 5959718 (-)
RNA-Seq ExpressionPI0002455
SyntenyPI0002455
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTACCAAAAGCAGAATCCTCATCGTTGGAGGCACTGGTTACATTGGTAAATTCATTGTCGAAGCAAGCGCCAAGGCCGGTCATCAAACGTACGCTCTGGTTCGAGATTCTGCTCCCTCGGATCCGGCCAAGTCTTTGATCATACAAAGCTTCAAAAGCATGGGAGTCATTCTTGTCTCTGTGAGCATTCCTTTTCTGTATAGGTTTTGAAATGAGTACTAAGGAGGAAATAATTTTTTTAAAAAACGAAAAATATTGTTTTATGGATGTTTAATTCATATGGAAATCTAATATTTTATTATTTTTTCTTTTAAATTTAGATTTTTTTTTCTTAAAAAATTATTTTGAGACTTGAATAAATTTTACTATATTTTGTAAATATTTAAAATTTTTCCGCAACTTAAATTTATATTTTATAAGAAAAATTATACAATAATATAATATCATTTCACTAGATATCCAAACTCAGATAATTACTATCTCATATAATTAAATATATCAATATCCATTAAAGAATATGTTTTAAGTCATTGAGATAATTTAAAAAGTATATGTAATGAATGAAGTTTTTAATTGTAGTTGTTATATATTGGAGTGGGTGCATGGTTGAGATAATTGATATATGTTGGAGATTGATTTGTAGGGCGATCTATTCGACCATGAGAGTTTGGTGAAGGCTATAAAGCAAGTGGATGTTGTTATATCAACCGTCGGTCTTACACAATTGGCTGATCAAGATAAGCTCATCTCTGCCATCAAAGAAGCAGGCAATGTCAAGGTTAATTACAATCATTAAACTATATATCTTTTCTCATTTTAAGTTCTTCCACTTTTCATTATTTCAAATAATAGTTAATCTCTTTTTACTTTATCGTTCTAATCCATAACTTGTAGAGAAGATATACCGATTGATTACTCAAAGCATGCAAATCTTCATTAAGAAATATACAAAAGGGTGAAATTCTATATAACATCTTCAGTTGAGGATATATATATATATATATAATATTAGTTAAGAAACGCTTAAAAAAGAAGTAAAAATTACTACCTATACCAACGTGTACCTTCTTTTTTAGTGACTCAATAATAGAATTTAACTTGGTGTAATTTTATGTTGGATAATCTTTTGAAATTTCTTTAAAAAACATGTGAACAAAGAAAGGAAGATGTTGCGAGGTTAGAGAGGAATGACCATATCGTTAGGTGTCGAATCTTTTAAGCCTAGGGCATTACAATAGTATCAACTCTACCTTACATAAATTTTCTTTCTTTTTCTCTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTGGTTTAGAGATTTTTTCCTTCGGAGTTTGGATATGATGTAGAAAATGTGCATGGAGTTGGGCCATTGATATCACTTTTTGAGACTAAGGTTCAAATTCGTAGAGCTGTGGAGGGTGGGGGAATTCCATACACCTATGTTTGGTGTTATTCATTTGATGGTTCCTATCTCCCTAGATTGGCTCAGCTTGAAGCCACTACTCCACCTAGAGACAAGGTTATTATCAAAGGAGATGGAAATCCTAAAGGTAAACTACTATTTTTCCCTCATAATTGCATCTTTTATTTCAAGCTAGGTACGTATAATAGGTCTAATTATATGTCGGCTTGAGGTCGTTTCTCCCGTATGTGATGAGGATTTGCCACATCTAAGTAAGAACTTGCATTTGTGTGGTATATTCTACTCGACACCCTCTAATGTCTAAGTTATATAGTATTGGACTAGAGTAATGTAGAGTTCAAGGTATATATTGATAGTGCTAATTGCAATGACAGATGGGTGGTCTATCTATTTATATGTTTTCTATATGACCTACGTATTTTTAAAAATGATATACGAGAGGCCAGAGAGATTAGTAGGTGTATATATCAAGATATACCCCTATCTCTGACATAGGTGTGTTAAAGTCCTTTATATGTTGTACTATTGATCAAAATTAAGGGAAGGATCCTAGCCTCCCCGAGTTGCCAATGGTTGGCTTGAAGGCCCTCTTCAATTTTGGGTAATGGCTTAGGCTCCTCACTTTGTGACTAGGAGCTCTCTGGCCTTTCTTATCATTTGGGTTCGTTGGATTTGCACGTTGTGTATAGTGAACCTAGATCTTGGGACCAGCCAGAAAAAGCCAATGACAATATGACTAAAAACCGTGTGTTATAAACAGAACCATTTGTTGGTATAAACCACAAAGTAGTTGTTATAAACTAAAATATTTTTCACTATAAACAAAAAGAAAGTGTTATAATAGACGAGAATATAGTAGCAATTTGCAGTTTGATTTGAGTAGTTTAAAAATCGTGGCAAGTAACCTCTTAAAACTACTTTTATTAGTGGTTGTAAAATCGCTGCTATAAATGTTGCATCAGTTAAAAACTATTGCAGGATAGTTTAAAAAACTACTACTATAGTCGTTGGTATTTCTTGTAGTGCTTAAAGATATAGAAATCTAAAAACTAAAATTGTAATCCTTGAAAATGATAAGGACTGAACAATTACCAAACTAAATATAAAAGTTTAGGATAAAAATAAGCTTTAAATCTTTGATAAAAAAGTGAATATTATCCTTAATTTAAATGTAAAAACAAAGTCCTTAAATTTTAGGGTTACGTTAGAGCAATGGACTTTGAGTTTATAATATTAGACATATTTCTCTTTTAAATTATATTGTCTGTCTAATATTAGCACAATGTCCTAAGAGTAGGGTATACTTTGTTGTAAATGTTTTCATAACATAAAATGAGTTTGTGTTTGTTTCCATTTTGTAGCAATTTTCAATAAGGAAGAAGATATTGCCTCCTACACCATCAAAGCTGTAGATGATCCGAGAACTTTAAACAAAGTCCTTTACATTAAGCCTCCTCAAAATATTCTCTCAATCAATGAACTTGTTGCTTTGTGGGAAAACAAAATTGGGAAAACCCTTGAAAAAACTTACATATCACAGGAACAATTACTAAAGAACATCCAAGGTTAGGATCGAAAATTAAGTATTGTTCTTCGAAATATAATTAAGATTCTGTTTGATAATTTTTTTTCGTTTTTTAAATTTATGTTTATTTTCTTTTAATGCCAACATGTTTTTTAAAACTACTTTTTGGTTTAATATTGTTGAAATATCTTCAAAATTTTATTAAGCGAATGGCCAAGCACGAGGGTGTTTTGCTACCTGAGATTAGCGAGACTAGGTCCATAAATAAATCATTTTAACGATCATAGTAACAATGCAATTCTTGAAAGAATAAGAGTATTTTCTTTTTTTATGAATTATCCTTAGTAGAGATTTTTCCAAATAAAGAAAAATAAATCAAAATATTTACAAATATAGCAAAAAATATTATTGTCTATTAGTGATAGACACCGATATCAACACCTTTTAGATATTTTGATATATTTGAAAATATTTTCAAAACCTTTGCTATTTAAAACAATTTTTCTTTTTATTAGAGTTTTTTCTTAAAAGAAAAACATTAAACAAAAAAGAGGAGACCATAGCAATATGAAGTTAAGGGCTCTGAATTTTTATATTTCACTGAAGCAAATATTGAAAATTGGAACTTAAAAGCTTAAGGGTTTATTGTTGTGCAGAAGCATTACCTCCATGGAATTTGCTACTGTCTTTAGATTACTCAGTTTTTGTGAAGGGAGATCACACCAACCATGAAGTTGAAGAATCAGTTGGATTGGAAGCTTCAAAGTTATATCCAGATGTTCAATATACTTCTGTTAGTGAATATCTCAATCAGTTTGTTTAA

mRNA sequence

ATGGCTACCAAAAGCAGAATCCTCATCGTTGGAGGCACTGGTTACATTGGTAAATTCATTGTCGAAGCAAGCGCCAAGGCCGGTCATCAAACGTACGCTCTGGTTCGAGATTCTGCTCCCTCGGATCCGGCCAAGTCTTTGATCATACAAAGCTTCAAAAGCATGGGAGTCATTCTTGTCTCTGGCGATCTATTCGACCATGAGAGTTTGGTGAAGGCTATAAAGCAAGTGGATGTTGTTATATCAACCGTCGGTCTTACACAATTGGCTGATCAAGATAAGCTCATCTCTGCCATCAAAGAAGCAGGCAATGTCAAGAGATTTTTTCCTTCGGAGTTTGGATATGATGTAGAAAATGTGCATGGAGTTGGGCCATTGATATCACTTTTTGAGACTAAGGTTCAAATTCGTAGAGCTGTGGAGGGTGGGGGAATTCCATACACCTATGTTTGGTGTTATTCATTTGATGGTTCCTATCTCCCTAGATTGGCTCAGCTTGAAGCCACTACTCCACCTAGAGACAAGGTTATTATCAAAGGAGATGGAAATCCTAAAGCAATTTTCAATAAGGAAGAAGATATTGCCTCCTACACCATCAAAGCTGTAGATGATCCGAGAACTTTAAACAAAGTCCTTTACATTAAGCCTCCTCAAAATATTCTCTCAATCAATGAACTTGTTGCTTTGTGGGAAAACAAAATTGGGAAAACCCTTGAAAAAACTTACATATCACAGGAACAATTACTAAAGAACATCCAAGAAGCATTACCTCCATGGAATTTGCTACTGTCTTTAGATTACTCAGTTTTTGTGAAGGGAGATCACACCAACCATGAAGTTGAAGAATCAGTTGGATTGGAAGCTTCAAAGTTATATCCAGATGTTCAATATACTTCTGTTAGTGAATATCTCAATCAGTTTGTTTAA

Coding sequence (CDS)

ATGGCTACCAAAAGCAGAATCCTCATCGTTGGAGGCACTGGTTACATTGGTAAATTCATTGTCGAAGCAAGCGCCAAGGCCGGTCATCAAACGTACGCTCTGGTTCGAGATTCTGCTCCCTCGGATCCGGCCAAGTCTTTGATCATACAAAGCTTCAAAAGCATGGGAGTCATTCTTGTCTCTGGCGATCTATTCGACCATGAGAGTTTGGTGAAGGCTATAAAGCAAGTGGATGTTGTTATATCAACCGTCGGTCTTACACAATTGGCTGATCAAGATAAGCTCATCTCTGCCATCAAAGAAGCAGGCAATGTCAAGAGATTTTTTCCTTCGGAGTTTGGATATGATGTAGAAAATGTGCATGGAGTTGGGCCATTGATATCACTTTTTGAGACTAAGGTTCAAATTCGTAGAGCTGTGGAGGGTGGGGGAATTCCATACACCTATGTTTGGTGTTATTCATTTGATGGTTCCTATCTCCCTAGATTGGCTCAGCTTGAAGCCACTACTCCACCTAGAGACAAGGTTATTATCAAAGGAGATGGAAATCCTAAAGCAATTTTCAATAAGGAAGAAGATATTGCCTCCTACACCATCAAAGCTGTAGATGATCCGAGAACTTTAAACAAAGTCCTTTACATTAAGCCTCCTCAAAATATTCTCTCAATCAATGAACTTGTTGCTTTGTGGGAAAACAAAATTGGGAAAACCCTTGAAAAAACTTACATATCACAGGAACAATTACTAAAGAACATCCAAGAAGCATTACCTCCATGGAATTTGCTACTGTCTTTAGATTACTCAGTTTTTGTGAAGGGAGATCACACCAACCATGAAGTTGAAGAATCAGTTGGATTGGAAGCTTCAAAGTTATATCCAGATGTTCAATATACTTCTGTTAGTGAATATCTCAATCAGTTTGTTTAA

Protein sequence

MATKSRILIVGGTGYIGKFIVEASAKAGHQTYALVRDSAPSDPAKSLIIQSFKSMGVILVSGDLFDHESLVKAIKQVDVVISTVGLTQLADQDKLISAIKEAGNVKRFFPSEFGYDVENVHGVGPLISLFETKVQIRRAVEGGGIPYTYVWCYSFDGSYLPRLAQLEATTPPRDKVIIKGDGNPKAIFNKEEDIASYTIKAVDDPRTLNKVLYIKPPQNILSINELVALWENKIGKTLEKTYISQEQLLKNIQEALPPWNLLLSLDYSVFVKGDHTNHEVEESVGLEASKLYPDVQYTSVSEYLNQFV
Homology
BLAST of PI0002455 vs. ExPASy Swiss-Prot
Match: O81355 (Phenylcoumaran benzylic ether reductase Pyrc5 OS=Pyrus communis OX=23211 GN=PYRC5 PE=1 SV=1)

HSP 1 Score: 440.7 bits (1132), Expect = 1.4e-122
Identity = 218/308 (70.78%), Postives = 258/308 (83.77%), Query Frame = 0

Query: 1   MATKSRILIVGGTGYIGKFIVEASAKAGHQTYALVRDSAPSDPAKSLIIQSFKSMGVILV 60
           MA+KS+IL +GGTGYIGKFIVEASAKAG+ TY LVR+++ SDPAKS +I++FK++GV  V
Sbjct: 1   MASKSQILFIGGTGYIGKFIVEASAKAGYPTYVLVREASLSDPAKSKVIENFKALGVNFV 60

Query: 61  SGDLFDHESLVKAIKQVDVVISTVGLTQLADQDKLISAIKEAGNVKRFFPSEFGYDVENV 120
            GDL+DHESLVKAIKQVDVVISTVG  QLADQ K+I+AIKEAGNVKRFFPSEFG DV+  
Sbjct: 61  LGDLYDHESLVKAIKQVDVVISTVGHGQLADQGKIIAAIKEAGNVKRFFPSEFGNDVDRS 120

Query: 121 HGVGPLISLFETKVQIRRAVEGGGIPYTYVWCYSFDGSYLPRLAQLEATTPPRDKVIIKG 180
           H V P  S FETK +IRRAVE  GIPYTYV    F G +LP L Q  A++ PRDKV+I G
Sbjct: 121 HAVEPAKSAFETKAKIRRAVEAEGIPYTYVSSNFFAGYFLPTLNQPGASSAPRDKVVILG 180

Query: 181 DGNPKAIFNKEEDIASYTIKAVDDPRTLNKVLYIKPPQNILSINELVALWENKIGKTLEK 240
           DGNPKAIFNKE+DI +YTI+AVDDPRTLNKVLYI+PP N +S NELV+LWE KIGKTLE+
Sbjct: 181 DGNPKAIFNKEDDIGTYTIRAVDDPRTLNKVLYIRPPANTISFNELVSLWEKKIGKTLER 240

Query: 241 TYISQEQLLKNIQEALPPWNLLLSLDYSVFVKGDHTNHEVEESVGLEASKLYPDVQYTSV 300
            Y+ +EQLLKNIQEA  P N++LS+ ++VFVKGDHTN E+E S G+EA+ LYPDV+YT+V
Sbjct: 241 IYVPEEQLLKNIQEAAVPLNVILSISHAVFVKGDHTNFEIEPSFGVEATALYPDVKYTTV 300

Query: 301 SEYLNQFV 309
            EYLNQFV
Sbjct: 301 DEYLNQFV 308

BLAST of PI0002455 vs. ExPASy Swiss-Prot
Match: B2WSN0 (Eugenol synthase 2 OS=Clarkia breweri OX=36903 GN=EGS2 PE=1 SV=1)

HSP 1 Score: 430.6 bits (1106), Expect = 1.5e-119
Identity = 212/309 (68.61%), Postives = 255/309 (82.52%), Query Frame = 0

Query: 1   MATKSRILIVGGTGYIGKFIVEASAKAGHQTYALVRDSAPSDPAKSLIIQSFKSMGVILV 60
           M +KS+ILI+GGTGYIGKFIVEAS K GH T+ALVR++  SDP K  +++ F+++GV L+
Sbjct: 1   MGSKSKILIIGGTGYIGKFIVEASVKEGHPTFALVRETTVSDPVKGKLVEKFQNLGVSLL 60

Query: 61  SGDLFDHESLVKAIKQVDVVISTVGLTQLADQDKLISAIKEAGNVKRFFPSEFGYDVENV 120
            GDL+DH+SLVKAIKQVDVVISTVG  Q+ADQ K+I+AIKEAGNVKRFFPSEFG DV++V
Sbjct: 61  YGDLYDHDSLVKAIKQVDVVISTVGFMQIADQTKIIAAIKEAGNVKRFFPSEFGNDVDHV 120

Query: 121 HGVGPLISL-FETKVQIRRAVEGGGIPYTYVWCYSFDGSYLPRLAQLEATTPPRDKVIIK 180
           + V P  S+ F  K  IRRAVE  GIPYTYV    F+G +LP L Q  ATTPPRDKVII 
Sbjct: 121 NAVEPAKSVAFAVKANIRRAVEAEGIPYTYVASNCFNGYFLPTLVQPGATTPPRDKVIIP 180

Query: 181 GDGNPKAIFNKEEDIASYTIKAVDDPRTLNKVLYIKPPQNILSINELVALWENKIGKTLE 240
           GDGNPKAIFNKEEDI +YTIKAVDDPRTLNK+LY++P  NI S NELVALWE KIGKTLE
Sbjct: 181 GDGNPKAIFNKEEDIGTYTIKAVDDPRTLNKILYLRPSNNIYSFNELVALWEKKIGKTLE 240

Query: 241 KTYISQEQLLKNIQEALPPWNLLLSLDYSVFVKGDHTNHEVEESVGLEASKLYPDVQYTS 300
           K Y+ +EQ+LK+IQEA  P N+ L +++SVFVKGDHTN E+E S G+EAS+LYP+V+YT+
Sbjct: 241 KIYVPEEQILKDIQEAPIPINIFLGINHSVFVKGDHTNFEIEPSFGVEASELYPEVKYTT 300

Query: 301 VSEYLNQFV 309
           V EYL+QFV
Sbjct: 301 VEEYLDQFV 309

BLAST of PI0002455 vs. ExPASy Swiss-Prot
Match: B9HRL7 (Phenylcoumaran benzylic ether reductase POP1 OS=Populus trichocarpa OX=3694 GN=PCBER PE=1 SV=1)

HSP 1 Score: 430.6 bits (1106), Expect = 1.5e-119
Identity = 211/304 (69.41%), Postives = 251/304 (82.57%), Query Frame = 0

Query: 5   SRILIVGGTGYIGKFIVEASAKAGHQTYALVRDSAPSDPAKSLIIQSFKSMGVILVSGDL 64
           S+IL +GGTGYIGKFIVEASAKAGH T+ LVR+S  S+PAKS++I +FK++GV  + GDL
Sbjct: 3   SKILFIGGTGYIGKFIVEASAKAGHPTFVLVRESTLSNPAKSVVIYNFKNLGVNFLIGDL 62

Query: 65  FDHESLVKAIKQVDVVISTVGLTQLADQDKLISAIKEAGNVKRFFPSEFGYDVENVHGVG 124
           FDHESLVKAIKQVDVVISTVG  QL +QD++I+AIKEAGNVKRFFPSEFG DV+ V+ V 
Sbjct: 63  FDHESLVKAIKQVDVVISTVGHAQLVEQDRIIAAIKEAGNVKRFFPSEFGNDVDRVNAVE 122

Query: 125 PLISLFETKVQIRRAVEGGGIPYTYVWCYSFDGSYLPRLAQLEATTPPRDKVIIKGDGNP 184
           P  S F TK  +RRA+E  GIPYTYV    F G +L    Q  AT PPRDKV+I GDGNP
Sbjct: 123 PAKSAFATKANVRRAIEAEGIPYTYVSSNFFSGYFLLSFNQPGATAPPRDKVVILGDGNP 182

Query: 185 KAIFNKEEDIASYTIKAVDDPRTLNKVLYIKPPQNILSINELVALWENKIGKTLEKTYIS 244
           KA+FNKE+DIA+YTIKAVDDPRTLNK+LYIKPP N +S N+LV+LWE KIGKTLE+ Y+ 
Sbjct: 183 KAVFNKEDDIATYTIKAVDDPRTLNKILYIKPPANTISFNDLVSLWEKKIGKTLERIYVP 242

Query: 245 QEQLLKNIQEALPPWNLLLSLDYSVFVKGDHTNHEVEESVGLEASKLYPDVQYTSVSEYL 304
           +EQLLKNIQEA  P N++LS+ +SVFVKGDHTN E+E S G+EAS+LYPDV+YT+V EYL
Sbjct: 243 EEQLLKNIQEASVPVNVVLSIGHSVFVKGDHTNFEIEPSFGVEASELYPDVKYTTVDEYL 302

Query: 305 NQFV 309
            QFV
Sbjct: 303 KQFV 306

BLAST of PI0002455 vs. ExPASy Swiss-Prot
Match: Q9FUW6 (Phenylcoumaran benzylic ether reductase Betv6 OS=Betula pendula OX=3505 GN=BETV6 PE=1 SV=1)

HSP 1 Score: 426.0 bits (1094), Expect = 3.6e-118
Identity = 207/308 (67.21%), Postives = 253/308 (82.14%), Query Frame = 0

Query: 1   MATKSRILIVGGTGYIGKFIVEASAKAGHQTYALVRDSAPSDPAKSLIIQSFKSMGVILV 60
           MA KS+ILI+GGTGYIGKFIVEASAK+GH T+ALVR+S  SDP K  +++ FK +GV L+
Sbjct: 1   MAHKSKILIIGGTGYIGKFIVEASAKSGHPTFALVRESTVSDPVKGKLVEKFKGLGVTLL 60

Query: 61  SGDLFDHESLVKAIKQVDVVISTVGLTQLADQDKLISAIKEAGNVKRFFPSEFGYDVENV 120
            GDL+DHESLVKA KQVDVVISTVG  QLADQ K+I+AIKEAGN+KRFFPSEFG DV+ V
Sbjct: 61  HGDLYDHESLVKAFKQVDVVISTVGHLQLADQVKIIAAIKEAGNIKRFFPSEFGNDVDRV 120

Query: 121 HGVGPLISLFETKVQIRRAVEGGGIPYTYVWCYSFDGSYLPRLAQLEATTPPRDKVIIKG 180
           H V P  + F TK +IRR  E  GIPYTYV    F G +LP LAQ   T+PPR+KV+I G
Sbjct: 121 HAVEPAKTAFATKAEIRRKTEAEGIPYTYVSSNFFAGYFLPTLAQPGLTSPPREKVVIFG 180

Query: 181 DGNPKAIFNKEEDIASYTIKAVDDPRTLNKVLYIKPPQNILSINELVALWENKIGKTLEK 240
           DGN +A+FNKE+DI +YTI+AVDDPRTLNK++YIKP +NI S NE+VALWE KIGKTLEK
Sbjct: 181 DGNARAVFNKEDDIGTYTIRAVDDPRTLNKIVYIKPAKNIYSFNEIVALWEKKIGKTLEK 240

Query: 241 TYISQEQLLKNIQEALPPWNLLLSLDYSVFVKGDHTNHEVEESVGLEASKLYPDVQYTSV 300
            Y+ +E+LLK+IQE+  P N++L++++SVFVKGDHTN E+E S G+EAS+LYPDV+YT+V
Sbjct: 241 IYVPEEKLLKDIQESPIPINVILAINHSVFVKGDHTNFEIEASFGVEASELYPDVKYTTV 300

Query: 301 SEYLNQFV 309
            EYL QFV
Sbjct: 301 EEYLQQFV 308

BLAST of PI0002455 vs. ExPASy Swiss-Prot
Match: B6VRE8 (Phenylcoumaran benzylic ether reductase TP7 OS=Nicotiana tabacum OX=4097 GN=TP7 PE=1 SV=1)

HSP 1 Score: 421.8 bits (1083), Expect = 6.8e-117
Identity = 203/308 (65.91%), Postives = 251/308 (81.49%), Query Frame = 0

Query: 1   MATKSRILIVGGTGYIGKFIVEASAKAGHQTYALVRDSAPSDPAKSLIIQSFKSMGVILV 60
           MA KS++LI+GGTGYIGKF+VEASAK+GH T+ALVR+S  SDP KS I+++FK++GV ++
Sbjct: 1   MAEKSKVLIIGGTGYIGKFVVEASAKSGHPTFALVRESTLSDPVKSKIVENFKNLGVTIL 60

Query: 61  SGDLFDHESLVKAIKQVDVVISTVGLTQLADQDKLISAIKEAGNVKRFFPSEFGYDVENV 120
            GDL+DHESLVKAIKQVDVVIST+G+ QL DQ KLI+AIKEAGN+KRFFPSEFG DV+  
Sbjct: 61  HGDLYDHESLVKAIKQVDVVISTMGMMQLGDQVKLIAAIKEAGNIKRFFPSEFGMDVDKT 120

Query: 121 HGVGPLISLFETKVQIRRAVEGGGIPYTYVWCYSFDGSYLPRLAQLEATTPPRDKVIIKG 180
           + V P  S F  KVQIRRA+E  GIPYTYV C  F G +LP + Q  AT PPRDKVII G
Sbjct: 121 NAVEPAKSAFAVKVQIRRAIEAEGIPYTYVSCNCFAGYFLPTMVQPGATVPPRDKVIIPG 180

Query: 181 DGNPKAIFNKEEDIASYTIKAVDDPRTLNKVLYIKPPQNILSINELVALWENKIGKTLEK 240
           DGN KA+FN+E DI +YTIKAVDDPRTLNK LYIKPP+N LS NELVA+WE  IGKTLEK
Sbjct: 181 DGNVKAVFNEEHDIGTYTIKAVDDPRTLNKTLYIKPPKNTLSFNELVAMWEKMIGKTLEK 240

Query: 241 TYISQEQLLKNIQEALPPWNLLLSLDYSVFVKGDHTNHEVEESVGLEASKLYPDVQYTSV 300
            YI +EQ+LK+I+ +  P  ++L+++++ FVKGD TN ++E S G+EAS+LYPDV+YT+V
Sbjct: 241 IYIPEEQILKDIETSPMPLPVILAINHATFVKGDQTNFKIEPSFGVEASELYPDVKYTTV 300

Query: 301 SEYLNQFV 309
            +YL  FV
Sbjct: 301 EDYLGHFV 308

BLAST of PI0002455 vs. ExPASy TrEMBL
Match: A0A6J1IIK0 (isoflavone reductase-like protein OS=Cucurbita maxima OX=3661 GN=LOC111474287 PE=4 SV=1)

HSP 1 Score: 493.8 bits (1270), Expect = 5.2e-136
Identity = 243/308 (78.90%), Postives = 272/308 (88.31%), Query Frame = 0

Query: 1   MATKSRILIVGGTGYIGKFIVEASAKAGHQTYALVRDSAPSDPAKSLIIQSFKSMGVILV 60
           MATKSR+L+VG TGYIGKFIVEAS K GHQT+ALVRDSA SDPAK  IIQ FKS+GVI+V
Sbjct: 1   MATKSRVLVVGSTGYIGKFIVEASVKYGHQTFALVRDSALSDPAKCPIIQKFKSIGVIVV 60

Query: 61  SGDLFDHESLVKAIKQVDVVISTVGLTQLADQDKLISAIKEAGNVKRFFPSEFGYDVENV 120
           SGDLFDHESLVKAIKQVDVVISTVGL QL+DQDKLISA+KEAGNVKRFFPSEFGYDVENV
Sbjct: 61  SGDLFDHESLVKAIKQVDVVISTVGLNQLSDQDKLISAVKEAGNVKRFFPSEFGYDVENV 120

Query: 121 HGVGPLISLFETKVQIRRAVEGGGIPYTYVWCYSFDGSYLPRLAQLEATTPPRDKVIIKG 180
           HGVGP+ S+FE+KVQIRRAVE  GIPYTYV  Y F+G  +P L QL  T PPRDKV+I G
Sbjct: 121 HGVGPVKSIFESKVQIRRAVEAEGIPYTYVLSYCFNGFCVPSLGQLGVTAPPRDKVVIHG 180

Query: 181 DGNPKAIFNKEEDIASYTIKAVDDPRTLNKVLYIKPPQNILSINELVALWENKIGKTLEK 240
           DGNPKAIFN EEDIAS+TIKAVDDPRTLNK+LYIKP +N+LSIN+LVALWENKIG  L K
Sbjct: 181 DGNPKAIFNTEEDIASFTIKAVDDPRTLNKILYIKPSENVLSINQLVALWENKIGIILHK 240

Query: 241 TYISQEQLLKNIQEALPPWNLLLSLDYSVFVKGDHTNHEVEESVGLEASKLYPDVQYTSV 300
           T++S+ QLLKNIQEA  P NL+L+ +YS+FVKGDHTN E+E SVGLEA+ LYPDV+YT+V
Sbjct: 241 TFVSEHQLLKNIQEAAYPLNLMLAFEYSIFVKGDHTNREIEASVGLEATSLYPDVKYTTV 300

Query: 301 SEYLNQFV 309
            EYLNQFV
Sbjct: 301 DEYLNQFV 308

BLAST of PI0002455 vs. ExPASy TrEMBL
Match: A0A1S3BJT1 (isoflavone reductase-like protein OS=Cucumis melo OX=3656 GN=LOC103490365 PE=4 SV=1)

HSP 1 Score: 486.1 bits (1250), Expect = 1.1e-133
Identity = 240/309 (77.67%), Postives = 271/309 (87.70%), Query Frame = 0

Query: 1   MATKSRILIVGGTGYIGKFIVEASAKAGHQTYALVRDSAPSDPAKSLIIQSFKSMGVILV 60
           MA KSR+L+VG TGYIGKFIVEASAKAGH T+ALVRDS+ SDPAKS IIQSFKS+GV +V
Sbjct: 1   MAAKSRVLVVGSTGYIGKFIVEASAKAGHPTFALVRDSSLSDPAKSQIIQSFKSIGVTIV 60

Query: 61  SGDLFDHESLVKAIKQVDVVISTVGLTQLADQDKLISAIKEAGNVKRFFPSEFGYDVENV 120
           +GDLF+HESLVKA+K+VDVVIS VGL QL+DQDKLISAIKEAGN+KRFFPSEFGYDVENV
Sbjct: 61  TGDLFEHESLVKAMKEVDVVISAVGLNQLSDQDKLISAIKEAGNIKRFFPSEFGYDVENV 120

Query: 121 HGVGPLISLFETKVQIRRAVEGGGIPYTYVWCYSFDGSYLPRLAQLEATTPPRDKVIIKG 180
           HG+G + S+FE KV+IRRAVE  GIPYTYV  Y F+G  LP LAQ  A  PP DKV+I+G
Sbjct: 121 HGIGQVKSIFEAKVKIRRAVEAEGIPYTYVLSYYFNGFCLPTLAQHGAAAPPTDKVVIQG 180

Query: 181 DGNPKAIFNKEEDIASYTIKAVDDPRTLNKVLYIKPPQNILSINELVALWENKIGKTLEK 240
           DGNPK IFNKEEDIASYTIKAVDDP  LNK+L+IKPPQN+LS N+LVALWENKIGKTL+K
Sbjct: 181 DGNPKVIFNKEEDIASYTIKAVDDPTALNKILHIKPPQNVLSFNQLVALWENKIGKTLDK 240

Query: 241 TYISQEQLLKNIQEALPPWNLLLSLDYSVFVKGDH-TNHEVEESVGLEASKLYPDVQYTS 300
           TYIS+ QLLKNIQEA  P NL+LS DYS+FVKGDH  NHE+E S GLEA+KLYPDV+YT+
Sbjct: 241 TYISEAQLLKNIQEAAYPLNLMLSFDYSIFVKGDHKANHEIEPSTGLEATKLYPDVKYTT 300

Query: 301 VSEYLNQFV 309
           V EYLNQFV
Sbjct: 301 VDEYLNQFV 309

BLAST of PI0002455 vs. ExPASy TrEMBL
Match: A0A6J1C1B8 (isoflavone reductase-like protein OS=Momordica charantia OX=3673 GN=LOC111007550 PE=4 SV=1)

HSP 1 Score: 480.7 bits (1236), Expect = 4.5e-132
Identity = 239/309 (77.35%), Postives = 269/309 (87.06%), Query Frame = 0

Query: 1   MATKSRILIVGGTGYIGKFIVEASAKAGHQTYALVRDSAPSDPAKSLIIQSFKSMGVILV 60
           MA KSR+L+VG TGYIGKFIVEASAKAGH TYALVRDSA SDPAKS IIQ F S GV +V
Sbjct: 1   MAGKSRVLVVGSTGYIGKFIVEASAKAGHPTYALVRDSALSDPAKSQIIQRFNSFGVTIV 60

Query: 61  SGDLFDHESLVKAIKQVDVVISTVGLTQLADQDKLISAIKEAGNVKRFFPSEFGYDVE-N 120
           SGDLFD ESLV AIKQVDVVISTVGL QL DQDKLI AIKEAGNVKRF PSEFGYDVE N
Sbjct: 61  SGDLFDRESLVNAIKQVDVVISTVGLNQLGDQDKLIYAIKEAGNVKRFLPSEFGYDVENN 120

Query: 121 VHGVGPLISLFETKVQIRRAVEGGGIPYTYVWCYSFDGSYLPRLAQLEATTPPRDKVIIK 180
           VHGVGP+ S+FE KV++RRAVE  GIPYTYVW Y F+G  LP LAQ  A+ PPRDKVIIK
Sbjct: 121 VHGVGPVKSIFEAKVRVRRAVEAEGIPYTYVWSYYFNGFCLPSLAQHGASAPPRDKVIIK 180

Query: 181 GDGNPKAIFNKEEDIASYTIKAVDDPRTLNKVLYIKPPQNILSINELVALWENKIGKTLE 240
           GDGNPK IFNKEEDIASYTI+A+DDPRTLNK++YIKPP+N+ S+NELVALWE KIGKTL+
Sbjct: 181 GDGNPKVIFNKEEDIASYTIRAMDDPRTLNKIVYIKPPENVFSLNELVALWETKIGKTLQ 240

Query: 241 KTYISQEQLLKNIQEALPPWNLLLSLDYSVFVKGDHTNHEVEESVGLEASKLYPDVQYTS 300
           K YIS++QLLKNIQE   P NL+L+ +YS+FVKGDH+N E+E SVGLEA++LYPD++YT+
Sbjct: 241 KIYISEDQLLKNIQE--DPENLMLAFEYSIFVKGDHSNREIEASVGLEATELYPDIEYTT 300

Query: 301 VSEYLNQFV 309
           V EYLNQFV
Sbjct: 301 VDEYLNQFV 307

BLAST of PI0002455 vs. ExPASy TrEMBL
Match: A0A1W5S651 (Phenylpropene reductase 1 OS=Malus domestica OX=3750 GN=PhR1 PE=2 SV=1)

HSP 1 Score: 445.7 bits (1145), Expect = 1.6e-121
Identity = 219/308 (71.10%), Postives = 260/308 (84.42%), Query Frame = 0

Query: 1   MATKSRILIVGGTGYIGKFIVEASAKAGHQTYALVRDSAPSDPAKSLIIQSFKSMGVILV 60
           MA+KS+IL +GGTGYIGKFIVEASAKAGH TY LVR++  S+PAKS +I++FK++GV  V
Sbjct: 1   MASKSKILFIGGTGYIGKFIVEASAKAGHSTYVLVRETTLSNPAKSKVIENFKALGVNFV 60

Query: 61  SGDLFDHESLVKAIKQVDVVISTVGLTQLADQDKLISAIKEAGNVKRFFPSEFGYDVENV 120
            GDL++HESLVKAIKQVDVVISTVG  QLADQ K+I+AIK+AGNVKRFFPSEFG DV+ V
Sbjct: 61  LGDLYNHESLVKAIKQVDVVISTVGHAQLADQGKIIAAIKKAGNVKRFFPSEFGNDVDRV 120

Query: 121 HGVGPLISLFETKVQIRRAVEGGGIPYTYVWCYSFDGSYLPRLAQLEATTPPRDKVIIKG 180
           H V P  S FETK +IRRAVE  GIPYTYV    F G +LP L Q  A++PPRDKV+I G
Sbjct: 121 HAVEPAKSAFETKAKIRRAVEAEGIPYTYVSSNFFAGYFLPTLNQPGASSPPRDKVVILG 180

Query: 181 DGNPKAIFNKEEDIASYTIKAVDDPRTLNKVLYIKPPQNILSINELVALWENKIGKTLEK 240
           DGNPKAIFNKE+DI +YTI+AVDDPRTLNKVLYI+PP N +S NELV+LWE KIGKTLE+
Sbjct: 181 DGNPKAIFNKEDDIGTYTIRAVDDPRTLNKVLYIRPPANTISFNELVSLWEKKIGKTLER 240

Query: 241 TYISQEQLLKNIQEALPPWNLLLSLDYSVFVKGDHTNHEVEESVGLEASKLYPDVQYTSV 300
            Y+ +EQLLKNIQEA+ P N++LS+ +SVFVKGDHTN E+E S G+EA+ LYPDV+YT+V
Sbjct: 241 IYVPEEQLLKNIQEAVVPLNVILSIGHSVFVKGDHTNFEIEPSFGVEATALYPDVKYTTV 300

Query: 301 SEYLNQFV 309
            EYLNQFV
Sbjct: 301 EEYLNQFV 308

BLAST of PI0002455 vs. ExPASy TrEMBL
Match: A0A067L047 (NmrA domain-containing protein OS=Jatropha curcas OX=180498 GN=JCGZ_26795 PE=3 SV=1)

HSP 1 Score: 444.1 bits (1141), Expect = 4.7e-121
Identity = 218/308 (70.78%), Postives = 257/308 (83.44%), Query Frame = 0

Query: 1   MATKSRILIVGGTGYIGKFIVEASAKAGHQTYALVRDSAPSDPAKSLIIQSFKSMGVILV 60
           MA KS+IL +GGTGYIGK+IVEASAKAGH T+ALVR+S+ SDPAK+ II  FKS+GV LV
Sbjct: 1   MAEKSKILFIGGTGYIGKYIVEASAKAGHPTFALVRESSLSDPAKAKIIDHFKSLGVNLV 60

Query: 61  SGDLFDHESLVKAIKQVDVVISTVGLTQLADQDKLISAIKEAGNVKRFFPSEFGYDVENV 120
           +GDL +HESLVKAIKQVDVVISTVG  QLADQ K+I+AIKEAGNVKRFFPSEFG DV+ V
Sbjct: 61  NGDLNNHESLVKAIKQVDVVISTVGHLQLADQGKIITAIKEAGNVKRFFPSEFGNDVDRV 120

Query: 121 HGVGPLISLFETKVQIRRAVEGGGIPYTYVWCYSFDGSYLPRLAQLEATTPPRDKVIIKG 180
           H V P  + FETK +IRR +E  GIPY+YV    F G +LP L Q   T PPRDKV+I G
Sbjct: 121 HAVEPAKTAFETKAKIRREIEAAGIPYSYVSSNFFAGYFLPSLNQPGLTAPPRDKVVILG 180

Query: 181 DGNPKAIFNKEEDIASYTIKAVDDPRTLNKVLYIKPPQNILSINELVALWENKIGKTLEK 240
           DGNPKA+FNKEEDI ++TIKAVDDPRTLNK+LYI+PP N +S NELV+LWE KIGKTLE+
Sbjct: 181 DGNPKAVFNKEEDIGTFTIKAVDDPRTLNKILYIRPPSNTISFNELVSLWEKKIGKTLER 240

Query: 241 TYISQEQLLKNIQEALPPWNLLLSLDYSVFVKGDHTNHEVEESVGLEASKLYPDVQYTSV 300
            Y+ +EQLLKNIQE+ PP N++L++ +SVFVKGDHTN E+E S G+EAS LYPDV+YT+V
Sbjct: 241 VYVPEEQLLKNIQESAPPVNVILAIGHSVFVKGDHTNFEIEPSFGVEASALYPDVKYTTV 300

Query: 301 SEYLNQFV 309
            EYLNQFV
Sbjct: 301 DEYLNQFV 308

BLAST of PI0002455 vs. NCBI nr
Match: XP_038887686.1 (phenylcoumaran benzylic ether reductase Pyrc5-like [Benincasa hispida])

HSP 1 Score: 498.0 bits (1281), Expect = 5.7e-137
Identity = 248/309 (80.26%), Postives = 278/309 (89.97%), Query Frame = 0

Query: 1   MATKSRILIVGGTGYIGKFIVEASAKAGHQTYALVRDSAPSDPAKSLIIQSFKSMGVILV 60
           MA+KSRIL+VG TGYIGKFIVEASAKAGH T+ALVRDSA SDPAKS IIQSFK++GVI+V
Sbjct: 1   MASKSRILVVGSTGYIGKFIVEASAKAGHPTFALVRDSALSDPAKSQIIQSFKNIGVIIV 60

Query: 61  SGDLFDHESLVKAIKQVDVVISTVGLTQLADQDKLISAIKEAGNVKRFFPSEFGYDVENV 120
           SGDLFDHESLVKAIK+VDVVISTVGL QL+DQDKLISAIKEAGNVKRFF SEFG+DVENV
Sbjct: 61  SGDLFDHESLVKAIKEVDVVISTVGLNQLSDQDKLISAIKEAGNVKRFFSSEFGFDVENV 120

Query: 121 HGVGPLISLFETKVQIRRAVEGGGIPYTYVWCYSFDGSYLPRLAQLEATTPPRDKVIIKG 180
           HG+GP+ S+FE KV+IRRAVE  GIPYTYV  Y F+G  LPRLAQ  AT PPRDKV+I+G
Sbjct: 121 HGIGPVKSIFEAKVKIRRAVEAEGIPYTYVLSYYFNGFCLPRLAQHGATAPPRDKVVIQG 180

Query: 181 DGNPKAIFNKEEDIASYTIKAVDDPRTLNKVLYIKPPQNILSINELVALWENKIGKTLEK 240
           DGNPK IFNKEEDIA+YTIKAVDDP TLNK+LYIKPPQN+LSINELVALWENKI KTL K
Sbjct: 181 DGNPKVIFNKEEDIATYTIKAVDDPTTLNKILYIKPPQNVLSINELVALWENKIAKTLHK 240

Query: 241 TYISQEQLLKNIQEALPPWNLLLSLDYSVFVKGDH-TNHEVEESVGLEASKLYPDVQYTS 300
           TYIS++QLLKNIQEA  P NL+L+ DYS+FVKGDH  N E+E SVGLEA+KLYP+V+YT+
Sbjct: 241 TYISEDQLLKNIQEATYPLNLMLAFDYSIFVKGDHKANQEIEASVGLEATKLYPEVKYTT 300

Query: 301 VSEYLNQFV 309
           V +YLNQFV
Sbjct: 301 VDQYLNQFV 309

BLAST of PI0002455 vs. NCBI nr
Match: XP_022975198.1 (isoflavone reductase-like protein [Cucurbita maxima])

HSP 1 Score: 493.8 bits (1270), Expect = 1.1e-135
Identity = 243/308 (78.90%), Postives = 272/308 (88.31%), Query Frame = 0

Query: 1   MATKSRILIVGGTGYIGKFIVEASAKAGHQTYALVRDSAPSDPAKSLIIQSFKSMGVILV 60
           MATKSR+L+VG TGYIGKFIVEAS K GHQT+ALVRDSA SDPAK  IIQ FKS+GVI+V
Sbjct: 1   MATKSRVLVVGSTGYIGKFIVEASVKYGHQTFALVRDSALSDPAKCPIIQKFKSIGVIVV 60

Query: 61  SGDLFDHESLVKAIKQVDVVISTVGLTQLADQDKLISAIKEAGNVKRFFPSEFGYDVENV 120
           SGDLFDHESLVKAIKQVDVVISTVGL QL+DQDKLISA+KEAGNVKRFFPSEFGYDVENV
Sbjct: 61  SGDLFDHESLVKAIKQVDVVISTVGLNQLSDQDKLISAVKEAGNVKRFFPSEFGYDVENV 120

Query: 121 HGVGPLISLFETKVQIRRAVEGGGIPYTYVWCYSFDGSYLPRLAQLEATTPPRDKVIIKG 180
           HGVGP+ S+FE+KVQIRRAVE  GIPYTYV  Y F+G  +P L QL  T PPRDKV+I G
Sbjct: 121 HGVGPVKSIFESKVQIRRAVEAEGIPYTYVLSYCFNGFCVPSLGQLGVTAPPRDKVVIHG 180

Query: 181 DGNPKAIFNKEEDIASYTIKAVDDPRTLNKVLYIKPPQNILSINELVALWENKIGKTLEK 240
           DGNPKAIFN EEDIAS+TIKAVDDPRTLNK+LYIKP +N+LSIN+LVALWENKIG  L K
Sbjct: 181 DGNPKAIFNTEEDIASFTIKAVDDPRTLNKILYIKPSENVLSINQLVALWENKIGIILHK 240

Query: 241 TYISQEQLLKNIQEALPPWNLLLSLDYSVFVKGDHTNHEVEESVGLEASKLYPDVQYTSV 300
           T++S+ QLLKNIQEA  P NL+L+ +YS+FVKGDHTN E+E SVGLEA+ LYPDV+YT+V
Sbjct: 241 TFVSEHQLLKNIQEAAYPLNLMLAFEYSIFVKGDHTNREIEASVGLEATSLYPDVKYTTV 300

Query: 301 SEYLNQFV 309
            EYLNQFV
Sbjct: 301 DEYLNQFV 308

BLAST of PI0002455 vs. NCBI nr
Match: KAG6572051.1 (Phenylcoumaran benzylic ether reductase Pyrc5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 491.5 bits (1264), Expect = 5.3e-135
Identity = 243/308 (78.90%), Postives = 271/308 (87.99%), Query Frame = 0

Query: 1   MATKSRILIVGGTGYIGKFIVEASAKAGHQTYALVRDSAPSDPAKSLIIQSFKSMGVILV 60
           MATKSR+L+VG TGYIGKFIVEAS K GHQT+ALVRDSA SDPAKS IIQ FKS+GVI+V
Sbjct: 1   MATKSRVLVVGSTGYIGKFIVEASVKYGHQTFALVRDSALSDPAKSPIIQKFKSIGVIVV 60

Query: 61  SGDLFDHESLVKAIKQVDVVISTVGLTQLADQDKLISAIKEAGNVKRFFPSEFGYDVENV 120
           SGDLFDHESLVKAIKQVDVVISTVGL QL+DQDKLISAIKEAGNVKRFFPSEFGYDVENV
Sbjct: 61  SGDLFDHESLVKAIKQVDVVISTVGLNQLSDQDKLISAIKEAGNVKRFFPSEFGYDVENV 120

Query: 121 HGVGPLISLFETKVQIRRAVEGGGIPYTYVWCYSFDGSYLPRLAQLEATTPPRDKVIIKG 180
           HGVGP+ S+FE+KVQIRRAVE  GIPYTYV  Y F+G  +P L QL  T PPRDKV+I G
Sbjct: 121 HGVGPVKSIFESKVQIRRAVEAEGIPYTYVLSYCFNGFCVPSLGQLGVTAPPRDKVVIHG 180

Query: 181 DGNPKAIFNKEEDIASYTIKAVDDPRTLNKVLYIKPPQNILSINELVALWENKIGKTLEK 240
           DGNPKAIFN EEDIAS+TIKAVDDP TLNK+LYIKP +N+LSIN+LVALWENKIG  L K
Sbjct: 181 DGNPKAIFNNEEDIASFTIKAVDDPTTLNKILYIKPSENVLSINQLVALWENKIGIILHK 240

Query: 241 TYISQEQLLKNIQEALPPWNLLLSLDYSVFVKGDHTNHEVEESVGLEASKLYPDVQYTSV 300
           T++S+ QLLKNIQEA  P NL+L+ +YS+FVKGDHTN  +E SVGLEA+ LYPDV+YT+V
Sbjct: 241 TFVSEHQLLKNIQEAAYPLNLMLAFEYSIFVKGDHTNRVIEASVGLEATSLYPDVKYTTV 300

Query: 301 SEYLNQFV 309
            EYLNQFV
Sbjct: 301 DEYLNQFV 308

BLAST of PI0002455 vs. NCBI nr
Match: XP_023511709.1 (isoflavone reductase homolog PCBER-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 488.4 bits (1256), Expect = 4.5e-134
Identity = 241/308 (78.25%), Postives = 270/308 (87.66%), Query Frame = 0

Query: 1   MATKSRILIVGGTGYIGKFIVEASAKAGHQTYALVRDSAPSDPAKSLIIQSFKSMGVILV 60
           MATKSR+L+VG TGYIGKFIVEAS K GH+T+ALVRDSA SDPAKS IIQ FKS+GVI+V
Sbjct: 1   MATKSRVLVVGSTGYIGKFIVEASVKYGHRTFALVRDSALSDPAKSPIIQKFKSIGVIVV 60

Query: 61  SGDLFDHESLVKAIKQVDVVISTVGLTQLADQDKLISAIKEAGNVKRFFPSEFGYDVENV 120
           SGDLFDHESLVKAIKQVDVVISTVGL QL+DQDKLISAIKEAGNVKRFFPSEFGYDVENV
Sbjct: 61  SGDLFDHESLVKAIKQVDVVISTVGLNQLSDQDKLISAIKEAGNVKRFFPSEFGYDVENV 120

Query: 121 HGVGPLISLFETKVQIRRAVEGGGIPYTYVWCYSFDGSYLPRLAQLEATTPPRDKVIIKG 180
           HGVGP+ S+FE+KVQIRRAVE  GIPYTYV  Y F+G  +P L QL  T PPRDKV+I G
Sbjct: 121 HGVGPVKSIFESKVQIRRAVEAEGIPYTYVLSYCFNGFCVPSLGQLGVTAPPRDKVVIHG 180

Query: 181 DGNPKAIFNKEEDIASYTIKAVDDPRTLNKVLYIKPPQNILSINELVALWENKIGKTLEK 240
           DGNPKAIFN EEDIAS+TIKAVDDP TLNK+LYIKP +N+LSIN+LVALWENKIG  L K
Sbjct: 181 DGNPKAIFNNEEDIASFTIKAVDDPTTLNKILYIKPSENVLSINQLVALWENKIGIILHK 240

Query: 241 TYISQEQLLKNIQEALPPWNLLLSLDYSVFVKGDHTNHEVEESVGLEASKLYPDVQYTSV 300
           T++S+ QLLKNI EA  P NL+L+ +YS+FVKGDHTN E+E SVGLEA+ LYPDV+Y +V
Sbjct: 241 TFVSEHQLLKNIHEAAYPLNLMLAFEYSIFVKGDHTNREIEASVGLEATSLYPDVKYMTV 300

Query: 301 SEYLNQFV 309
            EYLNQFV
Sbjct: 301 DEYLNQFV 308

BLAST of PI0002455 vs. NCBI nr
Match: XP_008448081.1 (PREDICTED: isoflavone reductase-like protein [Cucumis melo])

HSP 1 Score: 486.1 bits (1250), Expect = 2.2e-133
Identity = 240/309 (77.67%), Postives = 271/309 (87.70%), Query Frame = 0

Query: 1   MATKSRILIVGGTGYIGKFIVEASAKAGHQTYALVRDSAPSDPAKSLIIQSFKSMGVILV 60
           MA KSR+L+VG TGYIGKFIVEASAKAGH T+ALVRDS+ SDPAKS IIQSFKS+GV +V
Sbjct: 1   MAAKSRVLVVGSTGYIGKFIVEASAKAGHPTFALVRDSSLSDPAKSQIIQSFKSIGVTIV 60

Query: 61  SGDLFDHESLVKAIKQVDVVISTVGLTQLADQDKLISAIKEAGNVKRFFPSEFGYDVENV 120
           +GDLF+HESLVKA+K+VDVVIS VGL QL+DQDKLISAIKEAGN+KRFFPSEFGYDVENV
Sbjct: 61  TGDLFEHESLVKAMKEVDVVISAVGLNQLSDQDKLISAIKEAGNIKRFFPSEFGYDVENV 120

Query: 121 HGVGPLISLFETKVQIRRAVEGGGIPYTYVWCYSFDGSYLPRLAQLEATTPPRDKVIIKG 180
           HG+G + S+FE KV+IRRAVE  GIPYTYV  Y F+G  LP LAQ  A  PP DKV+I+G
Sbjct: 121 HGIGQVKSIFEAKVKIRRAVEAEGIPYTYVLSYYFNGFCLPTLAQHGAAAPPTDKVVIQG 180

Query: 181 DGNPKAIFNKEEDIASYTIKAVDDPRTLNKVLYIKPPQNILSINELVALWENKIGKTLEK 240
           DGNPK IFNKEEDIASYTIKAVDDP  LNK+L+IKPPQN+LS N+LVALWENKIGKTL+K
Sbjct: 181 DGNPKVIFNKEEDIASYTIKAVDDPTALNKILHIKPPQNVLSFNQLVALWENKIGKTLDK 240

Query: 241 TYISQEQLLKNIQEALPPWNLLLSLDYSVFVKGDH-TNHEVEESVGLEASKLYPDVQYTS 300
           TYIS+ QLLKNIQEA  P NL+LS DYS+FVKGDH  NHE+E S GLEA+KLYPDV+YT+
Sbjct: 241 TYISEAQLLKNIQEAAYPLNLMLSFDYSIFVKGDHKANHEIEPSTGLEATKLYPDVKYTT 300

Query: 301 VSEYLNQFV 309
           V EYLNQFV
Sbjct: 301 VDEYLNQFV 309

BLAST of PI0002455 vs. TAIR 10
Match: AT1G75280.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 403.7 bits (1036), Expect = 1.4e-112
Identity = 206/310 (66.45%), Postives = 245/310 (79.03%), Query Frame = 0

Query: 1   MAT-KSRILIVGGTGYIGKFIVEASAKAGHQTYALVRDSAPSDPAKSLIIQSFKSMGVIL 60
           MAT KS+IL++GGTGYIGKF+VEASAKAGH T+ALVR++  SDP K   +QSFK +GV +
Sbjct: 1   MATEKSKILVIGGTGYIGKFLVEASAKAGHSTFALVREATLSDPVKGKTVQSFKDLGVTI 60

Query: 61  VSGDLFDHESLVKAIKQVDVVISTVGLTQLADQDKLISAIKEAGNVKRFFPSEFGYDVEN 120
           + GDL DHESLVKAIKQVDVVISTVG  Q+ DQ K+ISAIKEAGNVKRF PSEFG DV+ 
Sbjct: 61  LHGDLNDHESLVKAIKQVDVVISTVGSMQILDQTKIISAIKEAGNVKRFLPSEFGVDVDR 120

Query: 121 VHGVGPLISLFETKVQIRRAVEGGGIPYTYVWCYSFDGSYLPRLAQLE--ATTPPRDKVI 180
              V P  S F  K+QIRR +E  GIPYTY     F G YLP L Q E   T+PPRDKV 
Sbjct: 121 TSAVEPAKSAFAGKIQIRRTIEAEGIPYTYAVTGCFGGYYLPTLVQFEPGLTSPPRDKVT 180

Query: 181 IKGDGNPKAIFNKEEDIASYTIKAVDDPRTLNKVLYIKPPQNILSINELVALWENKIGKT 240
           I GDGN KA+ NKEEDIA+YTIKAVDDPRTLNK+LYIKP  N LS+NE+V LWE KIGK+
Sbjct: 181 ILGDGNAKAVINKEEDIAAYTIKAVDDPRTLNKILYIKPSNNTLSMNEIVTLWEKKIGKS 240

Query: 241 LEKTYISQEQLLKNIQEALPPWNLLLSLDYSVFVKGDHTNHEVEESVGLEASKLYPDVQY 300
           LEKT++ +EQLLK+IQE+  P N++LS++++VFV GD TN  +E S G+EAS+LYPDV+Y
Sbjct: 241 LEKTHLPEEQLLKSIQESPIPINVVLSINHAVFVNGD-TNISIEPSFGVEASELYPDVKY 300

Query: 301 TSVSEYLNQF 308
           TSV EYL+ F
Sbjct: 301 TSVDEYLSYF 309

BLAST of PI0002455 vs. TAIR 10
Match: AT4G39230.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 401.4 bits (1030), Expect = 6.7e-112
Identity = 198/308 (64.29%), Postives = 243/308 (78.90%), Query Frame = 0

Query: 1   MATKSRILIVGGTGYIGKFIVEASAKAGHQTYALVRDSAPSDPAKSLIIQSFKSMGVILV 60
           M +KS+IL +GGTGYIGK+IVEASA++GH T  LVR+S  + P++S  I++FK++GV  +
Sbjct: 1   MTSKSKILFIGGTGYIGKYIVEASARSGHPTLVLVRNSTLTSPSRSSTIENFKNLGVQFL 60

Query: 61  SGDLFDHESLVKAIKQVDVVISTVGLTQLADQDKLISAIKEAGNVKRFFPSEFGYDVENV 120
            GDL DH SLV +IKQ DVVISTVG + L  Q K+ISAIKEAGNVKRFFPSEFG DV+ V
Sbjct: 61  LGDLDDHTSLVNSIKQADVVISTVGHSLLGHQYKIISAIKEAGNVKRFFPSEFGNDVDRV 120

Query: 121 HGVGPLISLFETKVQIRRAVEGGGIPYTYVWCYSFDGSYLPRLAQLEATTPPRDKVIIKG 180
             V P  S + TK +IRR +E  GIPYTYV C  F G +LP LAQ  AT+ PRDKVI+ G
Sbjct: 121 FTVEPAKSAYATKAKIRRTIEAEGIPYTYVSCNFFAGYFLPTLAQPGATSAPRDKVIVLG 180

Query: 181 DGNPKAIFNKEEDIASYTIKAVDDPRTLNKVLYIKPPQNILSINELVALWENKIGKTLEK 240
           DGNPKA+FNKEEDI +YTI AVDDPRTLNK+LYI+PP N  S N+LV+LWENKIGKTLE+
Sbjct: 181 DGNPKAVFNKEEDIGTYTINAVDDPRTLNKILYIRPPMNTYSFNDLVSLWENKIGKTLER 240

Query: 241 TYISQEQLLKNIQEALPPWNLLLSLDYSVFVKGDHTNHEVEESVGLEASKLYPDVQYTSV 300
            Y+ +EQLLK I E+ PP N++LSL + VFVKG HT+ E+E S G+EAS+LYPDV+YT+V
Sbjct: 241 IYVPEEQLLKQIIESSPPLNVMLSLCHCVFVKGGHTSFEIEPSFGVEASELYPDVKYTTV 300

Query: 301 SEYLNQFV 309
            E LNQ+V
Sbjct: 301 DEILNQYV 308

BLAST of PI0002455 vs. TAIR 10
Match: AT1G75290.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 394.8 bits (1013), Expect = 6.3e-110
Identity = 197/306 (64.38%), Postives = 238/306 (77.78%), Query Frame = 0

Query: 4   KSRILIVGGTGYIGKFIVEASAKAGHQTYALVRDSAPSDPAKSLIIQSFKSMGVILVSGD 63
           KS+IL++GGTG+IGK I+EAS KAGH T ALVR+++ SDP K   +Q+FK  GV L+ GD
Sbjct: 5   KSKILVIGGTGHIGKLIIEASVKAGHSTLALVREASLSDPNKGKTVQNFKDFGVTLLHGD 64

Query: 64  LFDHESLVKAIKQVDVVISTVGLTQLADQDKLISAIKEAGNVKRFFPSEFGYDVENVHGV 123
           L DHESLVKAIKQ DVVISTVG  Q+ DQ K+ISAIKEAGNVKRF PSEFG DV+    V
Sbjct: 65  LNDHESLVKAIKQADVVISTVGSMQILDQTKIISAIKEAGNVKRFLPSEFGMDVDKSSAV 124

Query: 124 GPLISLFETKVQIRRAVEGGGIPYTYVWCYSFDGSYLPRLAQLE--ATTPPRDKVIIKGD 183
            P  S F  K+Q RR +E  GIPYTY+    F G YLP L QLE   T+PPRDKV I GD
Sbjct: 125 EPAKSAFGRKLQTRRDIEAEGIPYTYLVTNYFAGYYLPTLVQLEPGLTSPPRDKVKIFGD 184

Query: 184 GNPKAIFNKEEDIASYTIKAVDDPRTLNKVLYIKPPQNILSINELVALWENKIGKTLEKT 243
           GN KA+ NKEEDIA+YTIKAVDDPRTLNK LYI PP N LS+NE+V LWE KIGK++EK 
Sbjct: 185 GNVKAVINKEEDIAAYTIKAVDDPRTLNKTLYINPPNNTLSMNEIVTLWEKKIGKSVEKI 244

Query: 244 YISQEQLLKNIQEALPPWNLLLSLDYSVFVKGDHTNHEVEESVGLEASKLYPDVQYTSVS 303
           Y+S+EQ+ K+IQE+  P+N+LLS++++VFVKGD TN  +E S G EAS+LYPD++YTS+ 
Sbjct: 245 YMSEEQIFKSIQESPVPFNVLLSINHAVFVKGDQTNFTIEPSFGFEASELYPDIKYTSID 304

Query: 304 EYLNQF 308
           EYL+ F
Sbjct: 305 EYLSYF 310

BLAST of PI0002455 vs. TAIR 10
Match: AT1G75300.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 375.2 bits (962), Expect = 5.2e-104
Identity = 196/320 (61.25%), Postives = 236/320 (73.75%), Query Frame = 0

Query: 4   KSRILIVGGTGYIGKFIVEASAKAGHQTYALVRDSAPSDPAKSLIIQSFKSMGVILVSGD 63
           KS+IL++GGTGY+G+FIVE SAKAG+ T+ALVR+++ SDP KS  IQSFK +GV ++ GD
Sbjct: 5   KSKILVIGGTGYMGEFIVEGSAKAGNPTFALVREASLSDPVKSKTIQSFKDLGVTILHGD 64

Query: 64  LFDHESLVKAIKQVDVVISTVGLTQLADQDKLISAIKEAGNVKRFFPSEFGYDVENVHGV 123
           L DHESLVKAIKQVDVVIST+G  Q+ DQ K+ISAIKEAGNVKRF P+EFG DVE    V
Sbjct: 65  LNDHESLVKAIKQVDVVISTIGHKQIFDQTKIISAIKEAGNVKRFLPAEFGIDVERTSAV 124

Query: 124 GPLISLFETKVQIRRAVEGGGIPYTYVWCYSFDGSYLPRLAQLEA--------------- 183
            P  SLF  KVQIRRA+E  GIPYTYV      G YL  L Q E+               
Sbjct: 125 EPAKSLFAGKVQIRRAIEAEGIPYTYVVSNCSAGFYLRTLLQFESGLISHTRDKAIIFGD 184

Query: 184 -TTPPRDKVIIKGDGNPKAIFNKEEDIASYTIKAVDDPRTLNKVLYIKPPQNILSINELV 243
              PPRDKV I GDGN K + NKEED+A+Y IKAVDD RTLNK LYI PP NILS+NE+V
Sbjct: 185 KNVPPRDKVTILGDGNAKVVINKEEDVAAYMIKAVDDLRTLNKTLYISPPNNILSMNEMV 244

Query: 244 ALWENKIGKTLEKTYISQEQLLKNIQEALPPWNLLLSLDYSVFVKGDHTNHEVEESVGLE 303
            LWE KIGK+LEKT+IS+EQ+LK+IQ    P ++  S++++VFVKGD T+  +E   G E
Sbjct: 245 TLWEKKIGKSLEKTHISEEQILKSIQ---VPIDVFKSINHAVFVKGDQTSFTIEPWFGEE 304

Query: 304 ASKLYPDVQYTSVSEYLNQF 308
           AS LYPDV+YTS+ EYL+QF
Sbjct: 305 ASVLYPDVKYTSIDEYLSQF 321

BLAST of PI0002455 vs. TAIR 10
Match: AT1G19540.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 353.2 bits (905), Expect = 2.1e-97
Identity = 177/308 (57.47%), Postives = 230/308 (74.68%), Query Frame = 0

Query: 5   SRILIVGGTGYIGKFIVEASAKAGHQTYALVRDSAPSDPAKSLIIQSFKSMGVILVSGDL 64
           S+IL++G TG IGK +VE SAK+GH T+ALVR+++ SDP K+ +++ FK +GV ++ G L
Sbjct: 3   SKILVIGATGLIGKVLVEESAKSGHATFALVREASLSDPVKAQLVERFKDLGVTILYGSL 62

Query: 65  FDHESLVKAIKQVDVVISTVG--LTQLADQDKLISAIKEAGNVKRFFPSEFGYDVENVHG 124
            D ESLVKAIKQVDVVIS VG   T++ +Q  +I AIKE+GNVKRF PSEFG DV+    
Sbjct: 63  SDKESLVKAIKQVDVVISAVGRFQTEILNQTNIIDAIKESGNVKRFLPSEFGNDVDRTVA 122

Query: 125 VGPLISLFETKVQIRRAVEGGGIPYTYVWCYSFDGSYLPRLAQ--LEATTPPRDKVIIKG 184
           + P +S F TK QIRRA+E   IPYTYV    F G ++P L Q  L   +PPRDKV I  
Sbjct: 123 IEPTLSEFITKAQIRRAIEAAKIPYTYVVSGCFAGLFVPCLGQCHLRLRSPPRDKVSIYD 182

Query: 185 DGNPKAIFNKEEDIASYTIKAVDDPRTLNKVLYIKPPQNILSINELVALWENKIGKTLEK 244
            GN KAI N EEDI +YT+KAVDDPRTLNK+LYI PP  I+S N++V LWE KIGKTLEK
Sbjct: 183 TGNGKAIVNTEEDIVAYTLKAVDDPRTLNKILYIHPPNYIVSQNDMVGLWEEKIGKTLEK 242

Query: 245 TYISQEQLLKNIQEALPPWNLLLSLDYSVFVKGDHTNHEVEESVGLEASKLYPDVQYTSV 304
           TY+S+E+LLK IQE+ PP + L+ L +++ VK D T+  ++ S G+EAS+LYP+V+YTSV
Sbjct: 243 TYVSEEELLKTIQESKPPMDFLVGLIHTILVKSDFTSFTIDPSFGVEASELYPEVKYTSV 302

Query: 305 SEYLNQFV 309
            E+LN+F+
Sbjct: 303 DEFLNRFI 310

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O813551.4e-12270.78Phenylcoumaran benzylic ether reductase Pyrc5 OS=Pyrus communis OX=23211 GN=PYRC... [more]
B2WSN01.5e-11968.61Eugenol synthase 2 OS=Clarkia breweri OX=36903 GN=EGS2 PE=1 SV=1[more]
B9HRL71.5e-11969.41Phenylcoumaran benzylic ether reductase POP1 OS=Populus trichocarpa OX=3694 GN=P... [more]
Q9FUW63.6e-11867.21Phenylcoumaran benzylic ether reductase Betv6 OS=Betula pendula OX=3505 GN=BETV6... [more]
B6VRE86.8e-11765.91Phenylcoumaran benzylic ether reductase TP7 OS=Nicotiana tabacum OX=4097 GN=TP7 ... [more]
Match NameE-valueIdentityDescription
A0A6J1IIK05.2e-13678.90isoflavone reductase-like protein OS=Cucurbita maxima OX=3661 GN=LOC111474287 PE... [more]
A0A1S3BJT11.1e-13377.67isoflavone reductase-like protein OS=Cucumis melo OX=3656 GN=LOC103490365 PE=4 S... [more]
A0A6J1C1B84.5e-13277.35isoflavone reductase-like protein OS=Momordica charantia OX=3673 GN=LOC111007550... [more]
A0A1W5S6511.6e-12171.10Phenylpropene reductase 1 OS=Malus domestica OX=3750 GN=PhR1 PE=2 SV=1[more]
A0A067L0474.7e-12170.78NmrA domain-containing protein OS=Jatropha curcas OX=180498 GN=JCGZ_26795 PE=3 S... [more]
Match NameE-valueIdentityDescription
XP_038887686.15.7e-13780.26phenylcoumaran benzylic ether reductase Pyrc5-like [Benincasa hispida][more]
XP_022975198.11.1e-13578.90isoflavone reductase-like protein [Cucurbita maxima][more]
KAG6572051.15.3e-13578.90Phenylcoumaran benzylic ether reductase Pyrc5, partial [Cucurbita argyrosperma s... [more]
XP_023511709.14.5e-13478.25isoflavone reductase homolog PCBER-like [Cucurbita pepo subsp. pepo][more]
XP_008448081.12.2e-13377.67PREDICTED: isoflavone reductase-like protein [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT1G75280.11.4e-11266.45NmrA-like negative transcriptional regulator family protein [more]
AT4G39230.16.7e-11264.29NmrA-like negative transcriptional regulator family protein [more]
AT1G75290.16.3e-11064.38NAD(P)-binding Rossmann-fold superfamily protein [more]
AT1G75300.15.2e-10461.25NmrA-like negative transcriptional regulator family protein [more]
AT1G19540.12.1e-9757.47NmrA-like negative transcriptional regulator family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.90.25.10coord: 154..304
e-value: 1.2E-131
score: 440.2
NoneNo IPR availableGENE3D3.40.50.720coord: 6..294
e-value: 1.2E-131
score: 440.2
NoneNo IPR availablePANTHERPTHR43349PINORESINOL REDUCTASE-RELATEDcoord: 3..308
NoneNo IPR availablePANTHERPTHR43349:SF76ISOFLAVONE REDUCTASE HOMOLOG P3-RELATEDcoord: 3..308
NoneNo IPR availableCDDcd05259PCBER_SDR_acoord: 6..303
e-value: 3.8295E-81
score: 245.29
IPR008030NmrA-like domainPFAMPF05368NmrAcoord: 7..265
e-value: 3.7E-78
score: 262.3
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 3..257

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0002455.1PI0002455.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009807 lignan biosynthetic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016491 oxidoreductase activity