PI0002396 (gene) Melon (PI 482460) v1

Overview
NamePI0002396
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptionshort-chain dehydrogenase reductase 2a
Locationchr08: 23152693 .. 23153936 (+)
RNA-Seq ExpressionPI0002396
SyntenyPI0002396
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTCTTTTGTATTAAGAAGTCTTGACTGTAAAGCCATGACTGCCCAAGTGCTTCCTGAACAATCCCTTCAGTCCAATATTCACCTCTTGGCTAGAGACAATAACTCCCCTCCACCATTCCATAAAAGGTACATTTTTCATTCATTATTTGTAGCTAATATAATTGTTATTTGATTTTAAAAGATCATAATAATGTTTATAATTTTTGTTTATTTTAGACTGGAGGGGAAAGTGGCCATCGTAACCGGGGGCGCTAAGGGAATCGGAGAAGCCACAGTTAGGCTATTTGCCAAACACGGAGCCAAAGTAGTAATCGCCGATGTGGAGGATATCCTCGGCCAGGCTTTGGCCAACACTCTTTCTCCATCCCCAGTATCCTTCGTCCACTGTGACGTCAGCTCCGAGGATGACGTGGAGAATCTAGTCAGCACCACCGTGTGCCTTCACGGCCAGGTGGACATCATATTCAACAACGCCGGTGTCCTTGGCAACCAATCCAAGTCCCACAAGAGCATCCTTGACTTTGACCCCGACGAGTTCGAGCGCGTCATGCGCGTGAATGTCAAGGGCGTAGCCTTGGGGATCAAGCACGCAGCGCGCGTAATGATCCCCAGAGCCACCGGATGCATAATCTCCACTGCCAGCGTGGCAGGGGTCTTGGGAGGCTTGGGCCCACACGCCTACACCGCATCTAAACACGCCATTGTCGGACTCACAAAGAACACAGCCTGCGAGCTCGGCCGATACGGCATACGTGTCAACTGCATTTCCCCATTTGGAGTTGCCACATCGATGCTCGTCAACGCGTGGAGAGCTGACGTGGAGGAAGACGAGCAATGTATGAACTTTGGGGTTCCTTCGGCGGCTGAGGTGGACAAAATGGAGGAGTTCGTTAGGGGTCTGGCCAATCTGAAGGGCCCCACATTGAGGCCGAAGGACATAGCTCAAGCCGCGCTTTATCTCGCCAGTGATGAATCCAAGTACATCAGTGGTCATAATCTCGTCGTTGATGGTGGAATTACCACTTCTAGGAATTGTATTGGCTTGTAACTTTTCTTTATTTTTTCTTTCTATTTTTTTTTCCAGGTATTGATAATATGCCTGTTGGTTGGTAGTTTTGGAGTAGGACTAATTATTATTATTATTTTTATTTTTTAATTGTTGTTTGTTGTTCTATAATTATTTCAACTACAATAGATGAATGGATATGCAAATATTAGTTAGTCCTTCTACCTCTATTCTAT

mRNA sequence

CTTTCTTTTGTATTAAGAAGTCTTGACTGTAAAGCCATGACTGCCCAAGTGCTTCCTGAACAATCCCTTCAGTCCAATATTCACCTCTTGGCTAGAGACAATAACTCCCCTCCACCATTCCATAAAAGACTGGAGGGGAAAGTGGCCATCGTAACCGGGGGCGCTAAGGGAATCGGAGAAGCCACAGTTAGGCTATTTGCCAAACACGGAGCCAAAGTAGTAATCGCCGATGTGGAGGATATCCTCGGCCAGGCTTTGGCCAACACTCTTTCTCCATCCCCAGTATCCTTCGTCCACTGTGACGTCAGCTCCGAGGATGACGTGGAGAATCTAGTCAGCACCACCGTGTGCCTTCACGGCCAGGTGGACATCATATTCAACAACGCCGGTGTCCTTGGCAACCAATCCAAGTCCCACAAGAGCATCCTTGACTTTGACCCCGACGAGTTCGAGCGCGTCATGCGCGTGAATGTCAAGGGCGTAGCCTTGGGGATCAAGCACGCAGCGCGCGTAATGATCCCCAGAGCCACCGGATGCATAATCTCCACTGCCAGCGTGGCAGGGGTCTTGGGAGGCTTGGGCCCACACGCCTACACCGCATCTAAACACGCCATTGTCGGACTCACAAAGAACACAGCCTGCGAGCTCGGCCGATACGGCATACGTGTCAACTGCATTTCCCCATTTGGAGTTGCCACATCGATGCTCGTCAACGCGTGGAGAGCTGACGTGGAGGAAGACGAGCAATGTATGAACTTTGGGGTTCCTTCGGCGGCTGAGGTGGACAAAATGGAGGAGTTCGTTAGGGGTCTGGCCAATCTGAAGGGCCCCACATTGAGGCCGAAGGACATAGCTCAAGCCGCGCTTTATCTCGCCAGTGATGAATCCAAGTACATCAGTGGTCATAATCTCGTCGTTGATGGTGGAATTACCACTTCTAGGAATTGTATTGGCTTGTAACTTTTCTTTATTTTTTCTTTCTATTTTTTTTTCCAGGTATTGATAATATGCCTGTTGGTTGGTAGTTTTGGAGTAGGACTAATTATTATTATTATTTTTATTTTTTAATTGTTGTTTGTTGTTCTATAATTATTTCAACTACAATAGATGAATGGATATGCAAATATTAGTTAGTCCTTCTACCTCTATTCTAT

Coding sequence (CDS)

ATGACTGCCCAAGTGCTTCCTGAACAATCCCTTCAGTCCAATATTCACCTCTTGGCTAGAGACAATAACTCCCCTCCACCATTCCATAAAAGACTGGAGGGGAAAGTGGCCATCGTAACCGGGGGCGCTAAGGGAATCGGAGAAGCCACAGTTAGGCTATTTGCCAAACACGGAGCCAAAGTAGTAATCGCCGATGTGGAGGATATCCTCGGCCAGGCTTTGGCCAACACTCTTTCTCCATCCCCAGTATCCTTCGTCCACTGTGACGTCAGCTCCGAGGATGACGTGGAGAATCTAGTCAGCACCACCGTGTGCCTTCACGGCCAGGTGGACATCATATTCAACAACGCCGGTGTCCTTGGCAACCAATCCAAGTCCCACAAGAGCATCCTTGACTTTGACCCCGACGAGTTCGAGCGCGTCATGCGCGTGAATGTCAAGGGCGTAGCCTTGGGGATCAAGCACGCAGCGCGCGTAATGATCCCCAGAGCCACCGGATGCATAATCTCCACTGCCAGCGTGGCAGGGGTCTTGGGAGGCTTGGGCCCACACGCCTACACCGCATCTAAACACGCCATTGTCGGACTCACAAAGAACACAGCCTGCGAGCTCGGCCGATACGGCATACGTGTCAACTGCATTTCCCCATTTGGAGTTGCCACATCGATGCTCGTCAACGCGTGGAGAGCTGACGTGGAGGAAGACGAGCAATGTATGAACTTTGGGGTTCCTTCGGCGGCTGAGGTGGACAAAATGGAGGAGTTCGTTAGGGGTCTGGCCAATCTGAAGGGCCCCACATTGAGGCCGAAGGACATAGCTCAAGCCGCGCTTTATCTCGCCAGTGATGAATCCAAGTACATCAGTGGTCATAATCTCGTCGTTGATGGTGGAATTACCACTTCTAGGAATTGTATTGGCTTGTAA

Protein sequence

MTAQVLPEQSLQSNIHLLARDNNSPPPFHKRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVVIADVEDILGQALANTLSPSPVSFVHCDVSSEDDVENLVSTTVCLHGQVDIIFNNAGVLGNQSKSHKSILDFDPDEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGGLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADVEEDEQCMNFGVPSAAEVDKMEEFVRGLANLKGPTLRPKDIAQAALYLASDESKYISGHNLVVDGGITTSRNCIGL
Homology
BLAST of PI0002396 vs. ExPASy Swiss-Prot
Match: Q9SCU0 (Short-chain dehydrogenase reductase 2a OS=Arabidopsis thaliana OX=3702 GN=SDR2a PE=3 SV=1)

HSP 1 Score: 401.0 bits (1029), Expect = 1.2e-110
Identity = 212/316 (67.09%), Postives = 251/316 (79.43%), Query Frame = 0

Query: 1   MTAQVLPEQSLQSNIHLLARDNNSPPPFHKRLEGKVAIVTGGAKGIGEATVRLFAKHGAK 60
           M AQV+ EQ+   ++H    +  +   + KRLEGKVAI+TGGA GIG+ATV LFA+HGA 
Sbjct: 1   MPAQVIAEQTTFHSVHDTIMEETNTTLYPKRLEGKVAIITGGAHGIGKATVMLFARHGAT 60

Query: 61  VVIADVEDILGQALANTLS---PSP-VSFVHCDVSSEDDVENLVSTTVCLHGQVDIIFNN 120
           VVIADV+++ G +LA +LS    SP V+F+ CDVS E DVENLV+ TV  +G++DI+FNN
Sbjct: 61  VVIADVDNVAGSSLAKSLSSHKTSPMVAFISCDVSVEADVENLVNVTVARYGRLDILFNN 120

Query: 121 AGVLGNQSKSHKSILDFDPDEFERVMRVNVKGVALGIKHAARVMIPRA-TGCIISTASVA 180
           AGVLG+Q K HKSILDFD DEF+ VMRVNV+GV LG+KH AR MI R   GCIISTASVA
Sbjct: 121 AGVLGDQ-KKHKSILDFDADEFDHVMRVNVRGVGLGMKHGARAMIKRGFKGCIISTASVA 180

Query: 181 GVLGGLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWR----AD 240
           GV+GG+GPHAYTASKHAIVGLTKN ACELG+YGIRVNCISPFGVATSMLVNAWR     D
Sbjct: 181 GVMGGMGPHAYTASKHAIVGLTKNAACELGKYGIRVNCISPFGVATSMLVNAWRKTSGGD 240

Query: 241 VEEDEQCMNFGVPSAAEVDKMEEFVRGLANLKGPTLRPKDIAQAALYLASDESKYISGHN 300
           VE+D            +V++MEEFVR LANLKG TLR  DIA+AALYLASDESKY++GHN
Sbjct: 241 VEDD------------DVEEMEEFVRSLANLKGETLRANDIAEAALYLASDESKYVNGHN 300

Query: 301 LVVDGGITTSRNCIGL 308
           LVVDGG+TT+RNC+GL
Sbjct: 301 LVVDGGVTTARNCVGL 303

BLAST of PI0002396 vs. ExPASy Swiss-Prot
Match: P50160 (Sex determination protein tasselseed-2 OS=Zea mays OX=4577 GN=TS2 PE=2 SV=1)

HSP 1 Score: 378.6 bits (971), Expect = 6.5e-104
Identity = 196/292 (67.12%), Postives = 230/292 (78.77%), Query Frame = 0

Query: 25  PPPFHKRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVVIADVEDILGQALANTLSPSPVS 84
           P P  KRL+GKVAIVTGGA+GIGEA VRLFAKHGA+VVIAD++D  G+ALA+ L P  VS
Sbjct: 46  PTPMPKRLDGKVAIVTGGARGIGEAIVRLFAKHGARVVIADIDDAAGEALASALGPQ-VS 105

Query: 85  FVHCDVSSEDDVENLVSTTVCLH-GQVDIIFNNAGVLGNQSKSHKSILDFDPDEFERVMR 144
           FV CDVS EDDV   V   +  H G++D+  NNAGVLG Q+++ +SIL FD  EF+RV+R
Sbjct: 106 FVRCDVSVEDDVRRAVDWALSRHGGRLDVYCNNAGVLGRQTRAARSILSFDAAEFDRVLR 165

Query: 145 VNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGGLGPHAYTASKHAIVGLTKNTACE 204
           VN  G ALG+KHAAR M PR  G I+S ASVA VLGGLGPHAYTASKHAIVGLTKN ACE
Sbjct: 166 VNALGAALGMKHAARAMAPRRAGSIVSVASVAAVLGGLGPHAYTASKHAIVGLTKNAACE 225

Query: 205 LGRYGIRVNCISPFGVATSMLVNAWR-------ADVEED-EQCMNFGVPSAAEVDKMEEF 264
           L  +G+RVNC+SPFGVAT ML+NAWR       AD + D +  ++  VPS  EV+KMEE 
Sbjct: 226 LRAHGVRVNCVSPFGVATPMLINAWRQGHDDATADADRDLDLDLDVTVPSDQEVEKMEEV 285

Query: 265 VRGLANLKGPTLRPKDIAQAALYLASDESKYISGHNLVVDGGITTSRNCIGL 308
           VRGLA LKGPTLRP+DIA+A L+LASDE++YISGHNLVVDGG+TTSRN IGL
Sbjct: 286 VRGLATLKGPTLRPRDIAEAVLFLASDEARYISGHNLVVDGGVTTSRNLIGL 336

BLAST of PI0002396 vs. ExPASy Swiss-Prot
Match: Q94KL7 (Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 PE=1 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 1.8e-69
Identity = 137/277 (49.46%), Postives = 187/277 (67.51%), Query Frame = 0

Query: 30  KRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVVIADVEDILGQALANTLSPSPVSFVHCD 89
           +RLEGKVA++TGGA GIGE T +LF++HGAKV IADV+D LG ++   +  S  +++HCD
Sbjct: 13  RRLEGKVALITGGASGIGETTAKLFSQHGAKVAIADVQDELGHSVVEAIGTSNSTYIHCD 72

Query: 90  VSSEDDVENLVSTTVCLHGQVDIIFNNAGVLGNQSKSHKSILDFDPDEFERVMRVNVKGV 149
           V++ED V+N V  TV  +G++DI+F+NAG+      +   I+D +  +FERV  VNV GV
Sbjct: 73  VTNEDGVKNAVDNTVSTYGKLDIMFSNAGI---SDPNRPRIIDNEKADFERVFSVNVTGV 132

Query: 150 ALGIKHAARVMIPRATGCIISTASVAGVLGGLGPHAYTASKHAIVGLTKNTACELGRYGI 209
            L +KHAARVMIP  +G IISTAS++  +GG   HAY  SKHA++GLT+N A ELG++GI
Sbjct: 133 FLCMKHAARVMIPARSGNIISTASLSSTMGGGSSHAYCGSKHAVLGLTRNLAVELGQFGI 192

Query: 210 RVNCISPFGVATSMLVN-AWRADVEEDEQCMNFGVPSAAEVDKMEEFVRGLANLKGPTLR 269
           RVNC+SPFG+ T++    +   + EE E  +NF                   NLKGP   
Sbjct: 193 RVNCLSPFGLPTALGKKFSGIKNEEEFENVINFA-----------------GNLKGPKFN 252

Query: 270 PKDIAQAALYLASDESKYISGHNLVVDGGITTSRNCI 306
            +D+A AALYLASDE+KY+SGHNL +DGG +   + I
Sbjct: 253 VEDVANAALYLASDEAKYVSGHNLFIDGGFSVCNSVI 269

BLAST of PI0002396 vs. ExPASy Swiss-Prot
Match: F1SWA0 (Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 4.4e-68
Identity = 136/276 (49.28%), Postives = 182/276 (65.94%), Query Frame = 0

Query: 31  RLEGKVAIVTGGAKGIGEATVRLFAKHGAKVVIADVEDILGQALANTLSPSP-VSFVHCD 90
           RLEGKVA+VTGGA GIGE+  RLF +HGAK+ I DV+D LGQ ++  L   P   + HCD
Sbjct: 2   RLEGKVALVTGGASGIGESIARLFIEHGAKICIVDVQDELGQQVSQRLGGDPHACYFHCD 61

Query: 91  VSSEDDVENLVSTTVCLHGQVDIIFNNAGVLGNQSKSHKSILDFDPDEFERVMRVNVKGV 150
           V+ EDDV   V  T   +G +DI+ NNAG+ G++      I D D +EF++V  +NV GV
Sbjct: 62  VTVEDDVRRAVDFTAEKYGTIDIMVNNAGITGDKV---IDIRDADFNEFKKVFDINVNGV 121

Query: 151 ALGIKHAARVMIPRATGCIISTASVAGVLGGLGPHAYTASKHAIVGLTKNTACELGRYGI 210
            LG+KHAAR+MIP+  G I+S ASV+ V+ G GPH YT +KHA+VGLTK+ A ELGR+GI
Sbjct: 122 FLGMKHAARIMIPKMKGSIVSLASVSSVIAGAGPHGYTGAKHAVVGLTKSVAAELGRHGI 181

Query: 211 RVNCISPFGVATSMLVNAWRADVEEDEQCMNFGVPSAAEVDKMEEFVRGLANLKGPTLRP 270
           RVNC+SP+ V T + +        +++    F             FVR  ANLKG  L P
Sbjct: 182 RVNCVSPYAVPTRLSMPYLPESEMQEDALRGF-----------LTFVRSNANLKGVDLMP 241

Query: 271 KDIAQAALYLASDESKYISGHNLVVDGGITTSRNCI 306
            D+A+A LYLA++ESKY+SG NLV+DGG + + + +
Sbjct: 242 NDVAEAVLYLATEESKYVSGLNLVIDGGFSIANHTL 263

BLAST of PI0002396 vs. ExPASy Swiss-Prot
Match: Q94KL8 (Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 PE=1 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 1.7e-67
Identity = 141/279 (50.54%), Postives = 184/279 (65.95%), Query Frame = 0

Query: 23  NSPPPFHKRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVVIADVEDILGQALANTL-SPS 82
           ++P     RL+ KVAI+TGGA GIGE T +LF ++GAKVVIAD+ D  GQ + N + SP 
Sbjct: 5   STPDSSTNRLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADIADDHGQKVCNNIGSPD 64

Query: 83  PVSFVHCDVSSEDDVENLVSTTVCLHGQVDIIFNNAGVLGNQSKSHKSILDFDPDEFERV 142
            +SFVHCDV+ ++DV NLV TT+  HG++DI+F N GVL   S +  SIL+   ++F+RV
Sbjct: 65  VISFVHCDVTKDEDVRNLVDTTIAKHGKLDIMFGNVGVL---STTPYSILEAGNEDFKRV 124

Query: 143 MRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGGLG-PHAYTASKHAIVGLTKNT 202
           M +NV G  L  KHAARVMIP   G I+ TAS++    G G  H YTA+KHA++GLT + 
Sbjct: 125 MDINVYGAFLVAKHAARVMIPAKKGSIVFTASISSFTAGEGVSHVYTATKHAVLGLTTSL 184

Query: 203 ACELGRYGIRVNCISPFGVATSMLVNAWRADVEEDEQCMNFGVPSAAEVDKMEEFVRGLA 262
             ELG YGIRVNC+SP+ VA+ +L +              FGV S+    ++EE     A
Sbjct: 185 CTELGEYGIRVNCVSPYIVASPLLTDV-------------FGVDSS----RVEELAHQAA 244

Query: 263 NLKGPTLRPKDIAQAALYLASDESKYISGHNLVVDGGIT 300
           NLKG  LR +D+A A  YLA DESKY+SG NLV+DGG T
Sbjct: 245 NLKGTLLRAEDVADAVAYLAGDESKYVSGLNLVIDGGYT 263

BLAST of PI0002396 vs. ExPASy TrEMBL
Match: A0A0A0LF57 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G730750 PE=4 SV=1)

HSP 1 Score: 595.1 bits (1533), Expect = 1.6e-166
Identity = 299/308 (97.08%), Postives = 305/308 (99.03%), Query Frame = 0

Query: 1   MTAQVLPEQSLQSNIHLLARDN-NSPPPFHKRLEGKVAIVTGGAKGIGEATVRLFAKHGA 60
           MTAQVLPEQSLQSNIHLL RDN +SPPPFHKRLEGKVAIVTGGAKGIGEATVRLFAKHGA
Sbjct: 1   MTAQVLPEQSLQSNIHLLVRDNSSSPPPFHKRLEGKVAIVTGGAKGIGEATVRLFAKHGA 60

Query: 61  KVVIADVEDILGQALANTLSPSPVSFVHCDVSSEDDVENLVSTTVCLHGQVDIIFNNAGV 120
           KVVIADVEDILGQALANTLSPSPVSFVHCDVSSEDDVENLVSTTVCLHGQVDIIFNNAGV
Sbjct: 61  KVVIADVEDILGQALANTLSPSPVSFVHCDVSSEDDVENLVSTTVCLHGQVDIIFNNAGV 120

Query: 121 LGNQSKSHKSILDFDPDEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLG 180
           LGNQS SHKSILDFDPDEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLG
Sbjct: 121 LGNQSNSHKSILDFDPDEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLG 180

Query: 181 GLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADVEEDEQCM 240
           GLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADVEE+E+CM
Sbjct: 181 GLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADVEEEEECM 240

Query: 241 NFGVPSAAEVDKMEEFVRGLANLKGPTLRPKDIAQAALYLASDESKYISGHNLVVDGGIT 300
           N+GVPSAAEVDKMEEFVRGLANLKGPTL+PKDIAQAALYLASDESKYISGHNLVVDGG+T
Sbjct: 241 NYGVPSAAEVDKMEEFVRGLANLKGPTLKPKDIAQAALYLASDESKYISGHNLVVDGGVT 300

Query: 301 TSRNCIGL 308
           TSRNCIGL
Sbjct: 301 TSRNCIGL 308

BLAST of PI0002396 vs. ExPASy TrEMBL
Match: A0A5A7TLW8 (Short-chain dehydrogenase reductase 2a OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G00390 PE=4 SV=1)

HSP 1 Score: 594.3 bits (1531), Expect = 2.8e-166
Identity = 298/307 (97.07%), Postives = 304/307 (99.02%), Query Frame = 0

Query: 1   MTAQVLPEQSLQSNIHLLARDNNSPPPFHKRLEGKVAIVTGGAKGIGEATVRLFAKHGAK 60
           MTAQVLPEQSLQSNIHLLARDNNSPPPFHKRLEGKVAIVTGGAKGIGEATVRLFAKHGAK
Sbjct: 1   MTAQVLPEQSLQSNIHLLARDNNSPPPFHKRLEGKVAIVTGGAKGIGEATVRLFAKHGAK 60

Query: 61  VVIADVEDILGQALANTLSPSPVSFVHCDVSSEDDVENLVSTTVCLHGQVDIIFNNAGVL 120
           VVIADVEDILGQALA+TLSPSPVSFVHCDVSSEDDVE LVSTTVCLHG VDIIFNNAGVL
Sbjct: 61  VVIADVEDILGQALADTLSPSPVSFVHCDVSSEDDVERLVSTTVCLHGHVDIIFNNAGVL 120

Query: 121 GNQSKSHKSILDFDPDEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGG 180
           G+QSKSHKSILDFDPDEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGG
Sbjct: 121 GSQSKSHKSILDFDPDEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGG 180

Query: 181 LGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADVEEDEQCMN 240
           LGPHAYTASKHAIVGLTKNTACELGR+GIRVNCISPFGVATSMLVNAWRADVEE++QCMN
Sbjct: 181 LGPHAYTASKHAIVGLTKNTACELGRHGIRVNCISPFGVATSMLVNAWRADVEEEDQCMN 240

Query: 241 FGVPSAAEVDKMEEFVRGLANLKGPTLRPKDIAQAALYLASDESKYISGHNLVVDGGITT 300
           +GVPSAAEVDKMEEFVRGLANLKGPTLRPKDIAQAALYLASDESKYISGHNLVVDGGITT
Sbjct: 241 YGVPSAAEVDKMEEFVRGLANLKGPTLRPKDIAQAALYLASDESKYISGHNLVVDGGITT 300

Query: 301 SRNCIGL 308
           S NCIGL
Sbjct: 301 STNCIGL 307

BLAST of PI0002396 vs. ExPASy TrEMBL
Match: A0A1S3B5H5 (short-chain dehydrogenase reductase 2a OS=Cucumis melo OX=3656 GN=LOC103486247 PE=4 SV=1)

HSP 1 Score: 594.3 bits (1531), Expect = 2.8e-166
Identity = 298/307 (97.07%), Postives = 304/307 (99.02%), Query Frame = 0

Query: 1   MTAQVLPEQSLQSNIHLLARDNNSPPPFHKRLEGKVAIVTGGAKGIGEATVRLFAKHGAK 60
           MTAQVLPEQSLQSNIHLLARDNNSPPPFHKRLEGKVAIVTGGAKGIGEATVRLFAKHGAK
Sbjct: 1   MTAQVLPEQSLQSNIHLLARDNNSPPPFHKRLEGKVAIVTGGAKGIGEATVRLFAKHGAK 60

Query: 61  VVIADVEDILGQALANTLSPSPVSFVHCDVSSEDDVENLVSTTVCLHGQVDIIFNNAGVL 120
           VVIADVEDILGQALA+TLSPSPVSFVHCDVSSEDDVE LVSTTVCLHG VDIIFNNAGVL
Sbjct: 61  VVIADVEDILGQALADTLSPSPVSFVHCDVSSEDDVERLVSTTVCLHGHVDIIFNNAGVL 120

Query: 121 GNQSKSHKSILDFDPDEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGG 180
           G+QSKSHKSILDFDPDEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGG
Sbjct: 121 GSQSKSHKSILDFDPDEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGG 180

Query: 181 LGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADVEEDEQCMN 240
           LGPHAYTASKHAIVGLTKNTACELGR+GIRVNCISPFGVATSMLVNAWRADVEE++QCMN
Sbjct: 181 LGPHAYTASKHAIVGLTKNTACELGRHGIRVNCISPFGVATSMLVNAWRADVEEEDQCMN 240

Query: 241 FGVPSAAEVDKMEEFVRGLANLKGPTLRPKDIAQAALYLASDESKYISGHNLVVDGGITT 300
           +GVPSAAEVDKMEEFVRGLANLKGPTLRPKDIAQAALYLASDESKYISGHNLVVDGGITT
Sbjct: 241 YGVPSAAEVDKMEEFVRGLANLKGPTLRPKDIAQAALYLASDESKYISGHNLVVDGGITT 300

Query: 301 SRNCIGL 308
           S NCIGL
Sbjct: 301 STNCIGL 307

BLAST of PI0002396 vs. ExPASy TrEMBL
Match: A0A6J1F7B6 (short-chain dehydrogenase reductase 2a-like OS=Cucurbita moschata OX=3662 GN=LOC111441518 PE=4 SV=1)

HSP 1 Score: 564.3 bits (1453), Expect = 3.1e-157
Identity = 281/307 (91.53%), Postives = 298/307 (97.07%), Query Frame = 0

Query: 1   MTAQVLPEQSLQSNIHLLARDNNSPPPFHKRLEGKVAIVTGGAKGIGEATVRLFAKHGAK 60
           MTAQVLPEQ+LQSNIHLL RDN+  P  HKRLEGKVAIVTGGAKGIGEATVRLFAKHGAK
Sbjct: 1   MTAQVLPEQALQSNIHLLGRDNS--PSLHKRLEGKVAIVTGGAKGIGEATVRLFAKHGAK 60

Query: 61  VVIADVEDILGQALANTLSPSPVSFVHCDVSSEDDVENLVSTTVCLHGQVDIIFNNAGVL 120
           VVIADVED+LGQALA+TLSPSPVSFVHCDVSSE+DVENL+S+TVC HGQVDIIFNNAGVL
Sbjct: 61  VVIADVEDLLGQALADTLSPSPVSFVHCDVSSEEDVENLISSTVCRHGQVDIIFNNAGVL 120

Query: 121 GNQSKSHKSILDFDPDEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGG 180
           GNQSK+HKSI+DFDP+EFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGG
Sbjct: 121 GNQSKTHKSIVDFDPNEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGG 180

Query: 181 LGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADVEEDEQCMN 240
           LGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRAD +E+++CMN
Sbjct: 181 LGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADGDEEDECMN 240

Query: 241 FGVPSAAEVDKMEEFVRGLANLKGPTLRPKDIAQAALYLASDESKYISGHNLVVDGGITT 300
            G+PS AEVDKMEEFVRGL+NLKGPTLRPKDIA+AALYLASDESKYISGHNLVVDGGITT
Sbjct: 241 LGIPSVAEVDKMEEFVRGLSNLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITT 300

Query: 301 SRNCIGL 308
           SRNCIGL
Sbjct: 301 SRNCIGL 305

BLAST of PI0002396 vs. ExPASy TrEMBL
Match: A0A6J1J5H2 (short-chain dehydrogenase reductase 2a-like OS=Cucurbita maxima OX=3661 GN=LOC111481921 PE=4 SV=1)

HSP 1 Score: 558.9 bits (1439), Expect = 1.3e-155
Identity = 279/307 (90.88%), Postives = 297/307 (96.74%), Query Frame = 0

Query: 1   MTAQVLPEQSLQSNIHLLARDNNSPPPFHKRLEGKVAIVTGGAKGIGEATVRLFAKHGAK 60
           MTAQVLPEQ+LQ+NI LL RDN+  P  HKRLEGKVAIVTGGAKGIGEATVRLFAKHGAK
Sbjct: 1   MTAQVLPEQALQTNILLLGRDNS--PSLHKRLEGKVAIVTGGAKGIGEATVRLFAKHGAK 60

Query: 61  VVIADVEDILGQALANTLSPSPVSFVHCDVSSEDDVENLVSTTVCLHGQVDIIFNNAGVL 120
           VVIADVED+LGQALA+TLSPSPVSFVHCDVSSE+DVENL+S+TVC HGQVDIIFNNAGVL
Sbjct: 61  VVIADVEDLLGQALADTLSPSPVSFVHCDVSSEEDVENLISSTVCRHGQVDIIFNNAGVL 120

Query: 121 GNQSKSHKSILDFDPDEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGG 180
           GNQSK+HKSI+DFDP+EFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGG
Sbjct: 121 GNQSKTHKSIVDFDPNEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGG 180

Query: 181 LGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADVEEDEQCMN 240
           LGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRAD +E+++CMN
Sbjct: 181 LGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADGDEEDECMN 240

Query: 241 FGVPSAAEVDKMEEFVRGLANLKGPTLRPKDIAQAALYLASDESKYISGHNLVVDGGITT 300
            G+PS AEVDKMEEFVRGL+NLKGPTLRPKDIA+AALYLASDESKYISGHNLVVDGGITT
Sbjct: 241 LGIPSVAEVDKMEEFVRGLSNLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITT 300

Query: 301 SRNCIGL 308
           SRNCIGL
Sbjct: 301 SRNCIGL 305

BLAST of PI0002396 vs. NCBI nr
Match: XP_004137687.1 (short-chain dehydrogenase reductase 2a [Cucumis sativus] >KGN58711.1 hypothetical protein Csa_002296 [Cucumis sativus])

HSP 1 Score: 595.1 bits (1533), Expect = 3.4e-166
Identity = 299/308 (97.08%), Postives = 305/308 (99.03%), Query Frame = 0

Query: 1   MTAQVLPEQSLQSNIHLLARDN-NSPPPFHKRLEGKVAIVTGGAKGIGEATVRLFAKHGA 60
           MTAQVLPEQSLQSNIHLL RDN +SPPPFHKRLEGKVAIVTGGAKGIGEATVRLFAKHGA
Sbjct: 1   MTAQVLPEQSLQSNIHLLVRDNSSSPPPFHKRLEGKVAIVTGGAKGIGEATVRLFAKHGA 60

Query: 61  KVVIADVEDILGQALANTLSPSPVSFVHCDVSSEDDVENLVSTTVCLHGQVDIIFNNAGV 120
           KVVIADVEDILGQALANTLSPSPVSFVHCDVSSEDDVENLVSTTVCLHGQVDIIFNNAGV
Sbjct: 61  KVVIADVEDILGQALANTLSPSPVSFVHCDVSSEDDVENLVSTTVCLHGQVDIIFNNAGV 120

Query: 121 LGNQSKSHKSILDFDPDEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLG 180
           LGNQS SHKSILDFDPDEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLG
Sbjct: 121 LGNQSNSHKSILDFDPDEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLG 180

Query: 181 GLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADVEEDEQCM 240
           GLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADVEE+E+CM
Sbjct: 181 GLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADVEEEEECM 240

Query: 241 NFGVPSAAEVDKMEEFVRGLANLKGPTLRPKDIAQAALYLASDESKYISGHNLVVDGGIT 300
           N+GVPSAAEVDKMEEFVRGLANLKGPTL+PKDIAQAALYLASDESKYISGHNLVVDGG+T
Sbjct: 241 NYGVPSAAEVDKMEEFVRGLANLKGPTLKPKDIAQAALYLASDESKYISGHNLVVDGGVT 300

Query: 301 TSRNCIGL 308
           TSRNCIGL
Sbjct: 301 TSRNCIGL 308

BLAST of PI0002396 vs. NCBI nr
Match: XP_008442351.1 (PREDICTED: short-chain dehydrogenase reductase 2a [Cucumis melo] >KAA0044210.1 short-chain dehydrogenase reductase 2a [Cucumis melo var. makuwa] >TYK24924.1 short-chain dehydrogenase reductase 2a [Cucumis melo var. makuwa])

HSP 1 Score: 594.3 bits (1531), Expect = 5.8e-166
Identity = 298/307 (97.07%), Postives = 304/307 (99.02%), Query Frame = 0

Query: 1   MTAQVLPEQSLQSNIHLLARDNNSPPPFHKRLEGKVAIVTGGAKGIGEATVRLFAKHGAK 60
           MTAQVLPEQSLQSNIHLLARDNNSPPPFHKRLEGKVAIVTGGAKGIGEATVRLFAKHGAK
Sbjct: 1   MTAQVLPEQSLQSNIHLLARDNNSPPPFHKRLEGKVAIVTGGAKGIGEATVRLFAKHGAK 60

Query: 61  VVIADVEDILGQALANTLSPSPVSFVHCDVSSEDDVENLVSTTVCLHGQVDIIFNNAGVL 120
           VVIADVEDILGQALA+TLSPSPVSFVHCDVSSEDDVE LVSTTVCLHG VDIIFNNAGVL
Sbjct: 61  VVIADVEDILGQALADTLSPSPVSFVHCDVSSEDDVERLVSTTVCLHGHVDIIFNNAGVL 120

Query: 121 GNQSKSHKSILDFDPDEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGG 180
           G+QSKSHKSILDFDPDEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGG
Sbjct: 121 GSQSKSHKSILDFDPDEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGG 180

Query: 181 LGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADVEEDEQCMN 240
           LGPHAYTASKHAIVGLTKNTACELGR+GIRVNCISPFGVATSMLVNAWRADVEE++QCMN
Sbjct: 181 LGPHAYTASKHAIVGLTKNTACELGRHGIRVNCISPFGVATSMLVNAWRADVEEEDQCMN 240

Query: 241 FGVPSAAEVDKMEEFVRGLANLKGPTLRPKDIAQAALYLASDESKYISGHNLVVDGGITT 300
           +GVPSAAEVDKMEEFVRGLANLKGPTLRPKDIAQAALYLASDESKYISGHNLVVDGGITT
Sbjct: 241 YGVPSAAEVDKMEEFVRGLANLKGPTLRPKDIAQAALYLASDESKYISGHNLVVDGGITT 300

Query: 301 SRNCIGL 308
           S NCIGL
Sbjct: 301 STNCIGL 307

BLAST of PI0002396 vs. NCBI nr
Match: XP_038905282.1 (short-chain dehydrogenase reductase 2a [Benincasa hispida])

HSP 1 Score: 570.9 bits (1470), Expect = 6.8e-159
Identity = 287/307 (93.49%), Postives = 297/307 (96.74%), Query Frame = 0

Query: 1   MTAQVLPEQSLQSNIHLLARDNNSPPPFHKRLEGKVAIVTGGAKGIGEATVRLFAKHGAK 60
           MTAQ+LPEQ LQ+NIHLL RDNNS P FHKRLEGKVAIVTGGAKGIGEATVRLFAKHGAK
Sbjct: 1   MTAQLLPEQPLQTNIHLLGRDNNS-PSFHKRLEGKVAIVTGGAKGIGEATVRLFAKHGAK 60

Query: 61  VVIADVEDILGQALANTLSPSPVSFVHCDVSSEDDVENLVSTTVCLHGQVDIIFNNAGVL 120
           VVIADVED+LGQ LA TLSP PVSFVHCDVSSE+DVENLVSTTVC HGQVDIIFNNAGVL
Sbjct: 61  VVIADVEDVLGQTLAETLSPFPVSFVHCDVSSEEDVENLVSTTVCRHGQVDIIFNNAGVL 120

Query: 121 GNQSKSHKSILDFDPDEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGG 180
           G+QSK+ KSILDFDPDEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGG
Sbjct: 121 GSQSKARKSILDFDPDEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGG 180

Query: 181 LGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADVEEDEQCMN 240
           LGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADV+E++QCMN
Sbjct: 181 LGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADVDEEDQCMN 240

Query: 241 FGVPSAAEVDKMEEFVRGLANLKGPTLRPKDIAQAALYLASDESKYISGHNLVVDGGITT 300
           FGVPSAAEVDKMEEFVRGLANLKGPTLRPKDIA+AALYLASDESKYISGHNLVVDGGITT
Sbjct: 241 FGVPSAAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITT 300

Query: 301 SRNCIGL 308
           S NCIGL
Sbjct: 301 SNNCIGL 306

BLAST of PI0002396 vs. NCBI nr
Match: XP_022934318.1 (short-chain dehydrogenase reductase 2a-like [Cucurbita moschata] >KAG6580441.1 Short-chain dehydrogenase reductase 2a, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 564.3 bits (1453), Expect = 6.4e-157
Identity = 281/307 (91.53%), Postives = 298/307 (97.07%), Query Frame = 0

Query: 1   MTAQVLPEQSLQSNIHLLARDNNSPPPFHKRLEGKVAIVTGGAKGIGEATVRLFAKHGAK 60
           MTAQVLPEQ+LQSNIHLL RDN+  P  HKRLEGKVAIVTGGAKGIGEATVRLFAKHGAK
Sbjct: 1   MTAQVLPEQALQSNIHLLGRDNS--PSLHKRLEGKVAIVTGGAKGIGEATVRLFAKHGAK 60

Query: 61  VVIADVEDILGQALANTLSPSPVSFVHCDVSSEDDVENLVSTTVCLHGQVDIIFNNAGVL 120
           VVIADVED+LGQALA+TLSPSPVSFVHCDVSSE+DVENL+S+TVC HGQVDIIFNNAGVL
Sbjct: 61  VVIADVEDLLGQALADTLSPSPVSFVHCDVSSEEDVENLISSTVCRHGQVDIIFNNAGVL 120

Query: 121 GNQSKSHKSILDFDPDEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGG 180
           GNQSK+HKSI+DFDP+EFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGG
Sbjct: 121 GNQSKTHKSIVDFDPNEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGG 180

Query: 181 LGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADVEEDEQCMN 240
           LGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRAD +E+++CMN
Sbjct: 181 LGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADGDEEDECMN 240

Query: 241 FGVPSAAEVDKMEEFVRGLANLKGPTLRPKDIAQAALYLASDESKYISGHNLVVDGGITT 300
            G+PS AEVDKMEEFVRGL+NLKGPTLRPKDIA+AALYLASDESKYISGHNLVVDGGITT
Sbjct: 241 LGIPSVAEVDKMEEFVRGLSNLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITT 300

Query: 301 SRNCIGL 308
           SRNCIGL
Sbjct: 301 SRNCIGL 305

BLAST of PI0002396 vs. NCBI nr
Match: XP_023528551.1 (short-chain dehydrogenase reductase 2a-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 562.8 bits (1449), Expect = 1.9e-156
Identity = 280/307 (91.21%), Postives = 297/307 (96.74%), Query Frame = 0

Query: 1   MTAQVLPEQSLQSNIHLLARDNNSPPPFHKRLEGKVAIVTGGAKGIGEATVRLFAKHGAK 60
           MTAQVLPEQ+LQSNIHLL RDN+  P  HKRLEGKVAIVTGGAKGIGEATVRLFAKHGAK
Sbjct: 93  MTAQVLPEQALQSNIHLLGRDNS--PSLHKRLEGKVAIVTGGAKGIGEATVRLFAKHGAK 152

Query: 61  VVIADVEDILGQALANTLSPSPVSFVHCDVSSEDDVENLVSTTVCLHGQVDIIFNNAGVL 120
           VVIADVED+LGQALA+TLSPSPVSFVHCDVSSE+DVENL+S+TVC HGQVDIIFNNAGVL
Sbjct: 153 VVIADVEDLLGQALADTLSPSPVSFVHCDVSSEEDVENLISSTVCRHGQVDIIFNNAGVL 212

Query: 121 GNQSKSHKSILDFDPDEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGG 180
           GNQSK+HKSI+DFDP+EFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGG
Sbjct: 213 GNQSKTHKSIVDFDPNEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGG 272

Query: 181 LGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADVEEDEQCMN 240
           LGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRAD +E+++CMN
Sbjct: 273 LGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADGDEEDECMN 332

Query: 241 FGVPSAAEVDKMEEFVRGLANLKGPTLRPKDIAQAALYLASDESKYISGHNLVVDGGITT 300
            G+PS  EVDKMEEFVRGL+NLKGPTLRPKDIA+AALYLASDESKYISGHNLVVDGGITT
Sbjct: 333 LGIPSVGEVDKMEEFVRGLSNLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITT 392

Query: 301 SRNCIGL 308
           SRNCIGL
Sbjct: 393 SRNCIGL 397

BLAST of PI0002396 vs. TAIR 10
Match: AT3G51680.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 401.0 bits (1029), Expect = 8.7e-112
Identity = 212/316 (67.09%), Postives = 251/316 (79.43%), Query Frame = 0

Query: 1   MTAQVLPEQSLQSNIHLLARDNNSPPPFHKRLEGKVAIVTGGAKGIGEATVRLFAKHGAK 60
           M AQV+ EQ+   ++H    +  +   + KRLEGKVAI+TGGA GIG+ATV LFA+HGA 
Sbjct: 1   MPAQVIAEQTTFHSVHDTIMEETNTTLYPKRLEGKVAIITGGAHGIGKATVMLFARHGAT 60

Query: 61  VVIADVEDILGQALANTLS---PSP-VSFVHCDVSSEDDVENLVSTTVCLHGQVDIIFNN 120
           VVIADV+++ G +LA +LS    SP V+F+ CDVS E DVENLV+ TV  +G++DI+FNN
Sbjct: 61  VVIADVDNVAGSSLAKSLSSHKTSPMVAFISCDVSVEADVENLVNVTVARYGRLDILFNN 120

Query: 121 AGVLGNQSKSHKSILDFDPDEFERVMRVNVKGVALGIKHAARVMIPRA-TGCIISTASVA 180
           AGVLG+Q K HKSILDFD DEF+ VMRVNV+GV LG+KH AR MI R   GCIISTASVA
Sbjct: 121 AGVLGDQ-KKHKSILDFDADEFDHVMRVNVRGVGLGMKHGARAMIKRGFKGCIISTASVA 180

Query: 181 GVLGGLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWR----AD 240
           GV+GG+GPHAYTASKHAIVGLTKN ACELG+YGIRVNCISPFGVATSMLVNAWR     D
Sbjct: 181 GVMGGMGPHAYTASKHAIVGLTKNAACELGKYGIRVNCISPFGVATSMLVNAWRKTSGGD 240

Query: 241 VEEDEQCMNFGVPSAAEVDKMEEFVRGLANLKGPTLRPKDIAQAALYLASDESKYISGHN 300
           VE+D            +V++MEEFVR LANLKG TLR  DIA+AALYLASDESKY++GHN
Sbjct: 241 VEDD------------DVEEMEEFVRSLANLKGETLRANDIAEAALYLASDESKYVNGHN 300

Query: 301 LVVDGGITTSRNCIGL 308
           LVVDGG+TT+RNC+GL
Sbjct: 301 LVVDGGVTTARNCVGL 303

BLAST of PI0002396 vs. TAIR 10
Match: AT3G29250.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 249.6 bits (636), Expect = 3.3e-66
Identity = 135/270 (50.00%), Postives = 180/270 (66.67%), Query Frame = 0

Query: 31  RLEGKVAIVTGGAKGIGEATVRLFAKHGAKVVIADVEDILGQALANTLSPSPVSFVHCDV 90
           RL+GK+AI+TGGA GIG   VRLF  HGAKVVI D+++ LGQ LA ++     SF  C+V
Sbjct: 5   RLDGKIAIITGGASGIGAEAVRLFTDHGAKVVIVDIQEELGQNLAVSIGLDKASFYRCNV 64

Query: 91  SSEDDVENLVSTTVCLHGQVDIIFNNAGVLGNQSKSHKSILDFDPDEFERVMRVNVKGVA 150
           + E DVEN V  TV  HG++D++F+NAGVL    ++  S+LD D + F+R M VNV+G A
Sbjct: 65  TDETDVENAVKFTVEKHGKLDVLFSNAGVL----EAFGSVLDLDLEAFDRTMAVNVRGAA 124

Query: 151 LGIKHAARVMIPRAT-GCIISTASVAGVLGGLGPHAYTASKHAIVGLTKNTACELGRYGI 210
             IKHAAR M+   T G I+ T S+A  +GG GPH+YTASKHA++GL ++    LG+YGI
Sbjct: 125 AFIKHAARSMVASGTRGSIVCTTSIAAEIGGPGPHSYTASKHALLGLIRSACAGLGQYGI 184

Query: 211 RVNCISPFGVATSMLVNAWRADVEEDEQCMNFGVPSAAEVDKMEEFVRGLANLKGPTLRP 270
           RVN ++P+GVAT M  +A+  +                 V  +EE+   L NLKG  L+ 
Sbjct: 185 RVNGVAPYGVATGM-TSAYNEEA----------------VKMLEEYGEALGNLKGVVLKA 244

Query: 271 KDIAQAALYLASDESKYISGHNLVVDGGIT 300
           + IA+AAL+LASD+S YISG NLVVDGG +
Sbjct: 245 RHIAEAALFLASDDSVYISGQNLVVDGGFS 253

BLAST of PI0002396 vs. TAIR 10
Match: AT3G29250.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 247.7 bits (631), Expect = 1.2e-65
Identity = 134/269 (49.81%), Postives = 179/269 (66.54%), Query Frame = 0

Query: 32  LEGKVAIVTGGAKGIGEATVRLFAKHGAKVVIADVEDILGQALANTLSPSPVSFVHCDVS 91
           L+GK+AI+TGGA GIG   VRLF  HGAKVVI D+++ LGQ LA ++     SF  C+V+
Sbjct: 44  LDGKIAIITGGASGIGAEAVRLFTDHGAKVVIVDIQEELGQNLAVSIGLDKASFYRCNVT 103

Query: 92  SEDDVENLVSTTVCLHGQVDIIFNNAGVLGNQSKSHKSILDFDPDEFERVMRVNVKGVAL 151
            E DVEN V  TV  HG++D++F+NAGVL    ++  S+LD D + F+R M VNV+G A 
Sbjct: 104 DETDVENAVKFTVEKHGKLDVLFSNAGVL----EAFGSVLDLDLEAFDRTMAVNVRGAAA 163

Query: 152 GIKHAARVMIPRAT-GCIISTASVAGVLGGLGPHAYTASKHAIVGLTKNTACELGRYGIR 211
            IKHAAR M+   T G I+ T S+A  +GG GPH+YTASKHA++GL ++    LG+YGIR
Sbjct: 164 FIKHAARSMVASGTRGSIVCTTSIAAEIGGPGPHSYTASKHALLGLIRSACAGLGQYGIR 223

Query: 212 VNCISPFGVATSMLVNAWRADVEEDEQCMNFGVPSAAEVDKMEEFVRGLANLKGPTLRPK 271
           VN ++P+GVAT M  +A+  +                 V  +EE+   L NLKG  L+ +
Sbjct: 224 VNGVAPYGVATGM-TSAYNEEA----------------VKMLEEYGEALGNLKGVVLKAR 283

Query: 272 DIAQAALYLASDESKYISGHNLVVDGGIT 300
            IA+AAL+LASD+S YISG NLVVDGG +
Sbjct: 284 HIAEAALFLASDDSVYISGQNLVVDGGFS 291

BLAST of PI0002396 vs. TAIR 10
Match: AT3G26770.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 246.5 bits (628), Expect = 2.8e-65
Identity = 131/270 (48.52%), Postives = 179/270 (66.30%), Query Frame = 0

Query: 30  KRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVVIADVEDILGQALANTLSPSPVSFVHCD 89
           K+LEGKVA++TGGA G+G+AT   F +HGA+VVIAD++   G   A  L  S   FV CD
Sbjct: 39  KKLEGKVALITGGASGLGKATASEFLRHGARVVIADLDAETGTKTAKELG-SEAEFVRCD 98

Query: 90  VSSEDDVENLVSTTVCLHGQVDIIFNNAGVLGNQSKSHKSILDFDPDEFERVMRVNVKGV 149
           V+ E D+   V  TV  +G++D+++NNAG++G  + +  SI   D  EFERVMR+NV GV
Sbjct: 99  VTVEADIAGAVEMTVERYGKLDVMYNNAGIVGPMTPA--SISQLDMTEFERVMRINVFGV 158

Query: 150 ALGIKHAARVMIPRATGCIISTASVAGVLGGLGPHAYTASKHAIVGLTKNTACELGRYGI 209
             GIKHAA+ MIP  +GCI+ T+SVAGV GGL PH+YT SK    G+ K+ A EL  +G+
Sbjct: 159 VSGIKHAAKFMIPARSGCILCTSSVAGVTGGLAPHSYTISKFTTPGIVKSAASELCEHGV 218

Query: 210 RVNCISPFGVATSMLVNAWRADVEEDEQCMNFGVPSAAEVDKMEEFVRGLANLKGPTLRP 269
           R+NCISP  VAT + ++            +    P  +E +K+ E V+G+  LKG     
Sbjct: 219 RINCISPGTVATPLTLS-----------YLQKVFPKVSE-EKLRETVKGMGELKGAECEE 278

Query: 270 KDIAQAALYLASDESKYISGHNLVVDGGIT 300
            D+A+AALYLAS++ KY++GHNLVVDGG+T
Sbjct: 279 ADVAKAALYLASNDGKYVTGHNLVVDGGMT 293

BLAST of PI0002396 vs. TAIR 10
Match: AT4G03140.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 245.4 bits (625), Expect = 6.1e-65
Identity = 125/271 (46.13%), Postives = 177/271 (65.31%), Query Frame = 0

Query: 30  KRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVVIADVEDILGQALANTLSPSPVSFVHCD 89
           ++LEGKVA++TGGA GIG+AT   F  HGAKV+IAD++  +G+     L PS  ++  CD
Sbjct: 76  RKLEGKVALITGGASGIGKATAGKFISHGAKVIIADIQPQIGRETEQELGPS-CAYFPCD 135

Query: 90  VSSEDDVENLVSTTVCLHGQVDIIFNNAGVLGNQSKSHKSILDFDPDEFERVMRVNVKGV 149
           V+ E D+ N V   V LH ++DI++NNAG+     K+  SI+D D + F++V+  NV+GV
Sbjct: 136 VTKESDIANAVDFAVSLHTKLDIMYNNAGI---PCKTPPSIVDLDLNVFDKVINTNVRGV 195

Query: 150 ALGIKHAARVMIPRATGCIISTASVAGVLGGLGPHAYTASKHAIVGLTKNTACELGRYGI 209
             GIKHAARVMIPR +G II   SV G++GGL  H Y+ SK A++G+ ++TA EL ++ I
Sbjct: 196 MAGIKHAARVMIPRNSGSIICAGSVTGMMGGLAQHTYSVSKSAVIGIVRSTASELCKHRI 255

Query: 210 RVNCISPFGVATSMLVNAWRADVEEDEQCMNFGVPSAAEVDKMEEFVRGLANLKGPTLRP 269
           RVNCISPF + TS +++  R             +    +  ++ + V+    L G    P
Sbjct: 256 RVNCISPFAITTSFVMDEMRQ------------IYPGVDDSRLIQIVQSTGVLNGEVCEP 315

Query: 270 KDIAQAALYLASDESKYISGHNLVVDGGITT 301
            D+A AA+YLASD+SKY++GHNLVVDGG TT
Sbjct: 316 TDVANAAVYLASDDSKYVNGHNLVVDGGFTT 330

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SCU01.2e-11067.09Short-chain dehydrogenase reductase 2a OS=Arabidopsis thaliana OX=3702 GN=SDR2a ... [more]
P501606.5e-10467.12Sex determination protein tasselseed-2 OS=Zea mays OX=4577 GN=TS2 PE=2 SV=1[more]
Q94KL71.8e-6949.46Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 P... [more]
F1SWA04.4e-6849.28Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1[more]
Q94KL81.7e-6750.54Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 P... [more]
Match NameE-valueIdentityDescription
A0A0A0LF571.6e-16697.08Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G730750 PE=4 SV=1[more]
A0A5A7TLW82.8e-16697.07Short-chain dehydrogenase reductase 2a OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3B5H52.8e-16697.07short-chain dehydrogenase reductase 2a OS=Cucumis melo OX=3656 GN=LOC103486247 P... [more]
A0A6J1F7B63.1e-15791.53short-chain dehydrogenase reductase 2a-like OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1J5H21.3e-15590.88short-chain dehydrogenase reductase 2a-like OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
Match NameE-valueIdentityDescription
XP_004137687.13.4e-16697.08short-chain dehydrogenase reductase 2a [Cucumis sativus] >KGN58711.1 hypothetica... [more]
XP_008442351.15.8e-16697.07PREDICTED: short-chain dehydrogenase reductase 2a [Cucumis melo] >KAA0044210.1 s... [more]
XP_038905282.16.8e-15993.49short-chain dehydrogenase reductase 2a [Benincasa hispida][more]
XP_022934318.16.4e-15791.53short-chain dehydrogenase reductase 2a-like [Cucurbita moschata] >KAG6580441.1 S... [more]
XP_023528551.11.9e-15691.21short-chain dehydrogenase reductase 2a-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G51680.18.7e-11267.09NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G29250.23.3e-6650.00NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G29250.11.2e-6549.81NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G26770.12.8e-6548.52NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G03140.16.1e-6546.13NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 262..282
score: 32.05
coord: 160..176
score: 33.98
coord: 36..53
score: 51.21
coord: 108..119
score: 51.1
coord: 186..205
score: 44.24
coord: 207..224
score: 41.2
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00080SDRFAMILYcoord: 108..119
score: 47.86
coord: 186..205
score: 38.49
coord: 166..174
score: 45.22
NoneNo IPR availableGENE3D3.40.50.720coord: 26..305
e-value: 1.9E-80
score: 271.8
NoneNo IPR availablePFAMPF13561adh_short_C2coord: 41..228
e-value: 3.0E-49
score: 167.7
coord: 247..299
e-value: 1.5E-10
score: 41.0
NoneNo IPR availablePIRSRPIRSR000095-1PIRSR000095-1coord: 38..230
e-value: 5.4E-17
score: 59.7
NoneNo IPR availablePIRSRPIRSR000094-3PIRSR000094-3coord: 31..219
e-value: 2.3E-7
score: 28.0
NoneNo IPR availablePANTHERPTHR43180:SF58SHORT-CHAIN DEHYDROGENASE REDUCTASE 2A-LIKEcoord: 1..307
NoneNo IPR availablePANTHERPTHR431803-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE (AFU_ORTHOLOGUE AFUA_6G11210)coord: 1..307
NoneNo IPR availableCDDcd05326secoisolariciresinol-DH_like_SDR_ccoord: 31..299
e-value: 1.23924E-130
score: 369.863
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 33..299

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0002396.1PI0002396.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004316 3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity
molecular_function GO:0016491 oxidoreductase activity