PI0002201 (gene) Melon (PI 482460) v1

BLAST of PI0002201 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 820.1 bits (2117), Expect = 2.1e-236
Identity = 395/738 (53.52%), Postives = 527/738 (71.41%), Query Frame = 0

Query: 30  FGSHFFGSNPSIYDVQLATESQYELLGSVVGSKLAAKESIFYSYNRYINGFAAILDENQA 89
           FG+H      +   +    E+ Y+ LGS  GS+  A ++IFYSY ++INGFAA LD + A
Sbjct: 36  FGAHSHVGEITEDAMDRVKETHYDFLGSFTGSRERATDAIFYSYTKHINGFAAHLDHDLA 95

Query: 90  NALARNPNVVSIFENQKRKLHTTRSWSFLGVENDEGIPPNSIWKAARFGEDTIIANLDTG 149
             ++++P VVS+F N+  KLHTTRSW FLG+E++  +P +SIW+ ARFGEDTIIANLDTG
Sbjct: 96  YEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHNSYVPSSSIWRKARFGEDTIIANLDTG 155

Query: 150 AWPESKSFNDAGYGPVPSRWMGVCEG--GSNFTCNKKLIGARYFNKGFEAENGPMSANLS 209
            WPESKSF D G GP+PSRW G+C+    + F CN+KLIGARYFNKG+ A  G ++++  
Sbjct: 156 VWPESKSFRDEGLGPIPSRWKGICQNQKDATFHCNRKLIGARYFNKGYAAAVGHLNSSFD 215

Query: 210 TARDQEGHGSHTLSTAGGNFVPGANVFGNGNGTAKGGSPKARLAAYKVCWPSFNGG-CYD 269
           + RD +GHGSHTLSTA G+FVPG ++FG GNGTAKGGSP+AR+AAYKVCWP   G  CYD
Sbjct: 216 SPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTAKGGSPRARVAAYKVCWPPVKGNECYD 275

Query: 270 ADILAAIEAAIHDGVDVLSISLGSSARDFASDTLSVGAFHAVQQGIVVVCSGGNDGPTPG 329
           AD+LAA +AAIHDG DV+S+SLG     F +D++++G+FHA ++ IVVVCS GN GP   
Sbjct: 276 ADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADS 335

Query: 330 TVTNVSPWMFTVAASTIDRDFVNYVALGNKRHFEGVSLSSGGLPRGKFYPLVDGVQVKAA 389
           TV+NV+PW  TV AST+DR+F + + LGN +H++G SLSS  LP  KFYP++  V  KA 
Sbjct: 336 TVSNVAPWQITVGASTMDREFASNLVLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKAK 395

Query: 390 NATDNLALLCEDGSLDPVKAKGKIVLCLRGDSARMDKSFEVRRAGGVGLILVNDKQDGND 449
           NA+   A LC+ GSLDP+K KGKI++CLRG + R++K   V   GG+G++L N    GND
Sbjct: 396 NASALDAQLCKLGSLDPIKTKGKILVCLRGQNGRVEKGRAVALGGGIGMVLENTYVTGND 455

Query: 450 ITADPHFLPASHLNYADGLAIFQYVNSTKSPMAFITHVKTELGIKPSPMVADFSSRGPNP 509
           + ADPH LPA+ L   D  A+ +Y++ TK P+A IT  +T+LG+KP+P++A FSS+GP+ 
Sbjct: 456 LLADPHVLPATQLTSKDSFAVSRYISQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSI 515

Query: 510 IIDSMIKPDIAAPGVSILAAFSKYVTATDFPLDTRRVPFNFESGTSMACPHISGVVGLLK 569
           +   ++KPDI APGVS++AA++  V+ T+   D RR+ FN  SGTSM+CPHISG+ GLLK
Sbjct: 516 VAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPRRLLFNAISGTSMSCPHISGIAGLLK 575

Query: 570 TLYPNWSPAAIKSAIMTTAKTRDNSMKSILDYNKVKATPFQYGAGHVHPNNAIDPGLVYD 629
           T YP+WSPAAI+SAIMTTA   D+    I +   +KATPF +GAGHV PN A++PGLVYD
Sbjct: 576 TRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATNMKATPFSFGAGHVQPNLAVNPGLVYD 635

Query: 630 TTIEDYMNFICAQGYNSTTLKKFYNKPFLCPK-SFALADLNYPSISVPKLTAGVPATINR 689
             I+DY+NF+C+ GYN++ +  F    F C     +L +LNYPSI+VP LT+    T++R
Sbjct: 636 LGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPKISLVNLNYPSITVPNLTSS-KVTVSR 695

Query: 690 RLKNVGTPGTYVARVKVSSKVSVTVKPSTLQFNSVGEEKAFKVVFEYKGKGIGKGHVFGT 749
            +KNVG P  Y  +V     V V VKP++L F  VGE+K FKV+       + KG+VFG 
Sbjct: 696 TVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKVGEQKTFKVILVKSKGNVAKGYVFGE 755

Query: 750 LIWSDGKHFVRSPMAVAL 764
           L+WSD KH VRSP+ V L
Sbjct: 756 LVWSDKKHRVRSPIVVKL 772

BLAST of PI0002201 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 808.5 bits (2087), Expect = 6.4e-233
Identity = 409/737 (55.50%), Postives = 513/737 (69.61%), Query Frame = 0

Query: 31  GSHFFGSNPSIYDVQLATESQYELLGSVVGSKLAAKESIFYSYNRYINGFAAILDENQAN 90
           GSH      S   +     S    L S VGS   AKE+IFYSY R+INGFAAILDEN+A 
Sbjct: 47  GSHAHLPQISSAHLDGVAHSHRTFLASFVGSHENAKEAIFYSYKRHINGFAAILDENEAA 106

Query: 91  ALARNPNVVSIFENQKRKLHTTRSWSFLGVENDEGIPPNSIWKAARFGEDTIIANLDTGA 150
            +A++P+VVS+F N+ RKLHTT SW+F+ +  +  +  +S+W  A +GEDTIIANLDTG 
Sbjct: 107 EIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKNGVVHKSSLWNKAGYGEDTIIANLDTGV 166

Query: 151 WPESKSFNDAGYGPVPSRWMGVCEGGSNFTCNKKLIGARYFNKGFEAENG-PMSANLSTA 210
           WPESKSF+D GYG VP+RW G C    +  CN+KLIGARYFNKG+ A  G P +A+  T 
Sbjct: 167 WPESKSFSDEGYGAVPARWKGRCH--KDVPCNRKLIGARYFNKGYLAYTGLPSNASYETC 226

Query: 211 RDQEGHGSHTLSTAGGNFVPGANVFGNGNGTAKGGSPKARLAAYKVCWPSFNGG-CYDAD 270
           RD +GHGSHTLSTA GNFVPGANVFG GNGTA GGSPKAR+AAYKVCWP  +G  C+DAD
Sbjct: 227 RDHDGHGSHTLSTAAGNFVPGANVFGIGNGTASGGSPKARVAAYKVCWPPVDGAECFDAD 286

Query: 271 ILAAIEAAIHDGVDVLSISLGSSARDFASDTLSVGAFHAVQQGIVVVCSGGNDGPTPGTV 330
           ILAAIEAAI DGVDVLS S+G  A D+ SD +++G+FHAV+ G+ VVCS GN GP  GTV
Sbjct: 287 ILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTV 346

Query: 331 TNVSPWMFTVAASTIDRDFVNYVALGNKRHFEGVSLSSGGLPRGKFYPLVDGVQVKAANA 390
           +NV+PW+ TV AS++DR+F  +V L N + F+G SLS   LP  K Y L+       AN 
Sbjct: 347 SNVAPWVITVGASSMDREFQAFVELKNGQSFKGTSLSK-PLPEEKMYSLISAADANVANG 406

Query: 391 TDNLALLCEDGSLDPVKAKGKIVLCLRGDSARMDKSFEVRRAGGVGLILVNDKQDGNDIT 450
               ALLC+ GSLDP K KGKI++CLRGD+AR+DK  +   AG  G++L NDK  GN+I 
Sbjct: 407 NVTDALLCKKGSLDPKKVKGKILVCLRGDNARVDKGMQAAAAGAAGMVLCNDKASGNEII 466

Query: 451 ADPHFLPASHLNYADGLAIFQYVNSTKSPMAFITHVKTELGIKPSPMVADFSSRGPNPII 510
           +D H LPAS ++Y DG  +F Y++STK P  +I      L  KP+P +A FSSRGPN I 
Sbjct: 467 SDAHVLPASQIDYKDGETLFSYLSSTKDPKGYIKAPTATLNTKPAPFMASFSSRGPNTIT 526

Query: 511 DSMIKPDIAAPGVSILAAFSKYVTATDFPLDTRRVPFNFESGTSMACPHISGVVGLLKTL 570
             ++KPDI APGV+I+AAF++    TD   D RR PFN ESGTSM+CPHISGVVGLLKTL
Sbjct: 527 PGILKPDITAPGVNIIAAFTEATGPTDLDSDNRRTPFNTESGTSMSCPHISGVVGLLKTL 586

Query: 571 YPNWSPAAIKSAIMTTAKTRDNSMKSILDYNKVKATPFQYGAGHVHPNNAIDPGLVYDTT 630
           +P+WSPAAI+SAIMTT++TR+N  K ++D +  KA PF YG+GHV PN A  PGLVYD T
Sbjct: 587 HPHWSPAAIRSAIMTTSRTRNNRRKPMVDESFKKANPFSYGSGHVQPNKAAHPGLVYDLT 646

Query: 631 IEDYMNFICAQGYNSTTLKKFYNKP-FLCPKSFALADLNYPSISVPKLTAGVPATINRRL 690
             DY++F+CA GYN+T ++ F   P + C +   L D NYPSI+VP LT  +  T+ R+L
Sbjct: 647 TGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGANLLDFNYPSITVPNLTGSI--TVTRKL 706

Query: 691 KNVGTPGTYVARVKVSSKVSVTVKPSTLQFNSVGEEKAFKVVFEYKGKGIGKGHVFGTLI 750
           KNVG P TY AR +    V V+V+P  L FN  GE K F++           G+VFG L 
Sbjct: 707 KNVGPPATYNARFREPLGVRVSVEPKQLTFNKTGEVKIFQMTLR-PLPVTPSGYVFGELT 766

Query: 751 WSDGKHFVRSPMAVALA 765
           W+D  H+VRSP+ V L+
Sbjct: 767 WTDSHHYVRSPIVVQLS 777

BLAST of PI0002201 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 716.1 bits (1847), Expect = 4.3e-205
Identity = 380/756 (50.26%), Postives = 502/756 (66.40%), Query Frame = 0

Query: 31  GSHFFGSNPSIYDVQLATESQYELLGSVVGSKLAAKESIFYSYNRYINGFAAILDENQAN 90
           G+H  G +P+  D++LAT+S Y+LLGS+ GS+  AKE+I YSYNR+INGFAA+L+E +A 
Sbjct: 37  GAHSHGPSPTSADLELATDSHYDLLGSIFGSREKAKEAIIYSYNRHINGFAALLEEEEAA 96

Query: 91  ALARNPNVVSIFENQKRKLHTTRSWSFLGVENDEGIPPNSIWKAARFGEDTIIANLDTGA 150
            +A+NPNVVS+F +++ KLHTTRSW FLG+        NS W+  RFGE+TII N+DTG 
Sbjct: 97  DIAKNPNVVSVFLSKEHKLHTTRSWEFLGLHRR---GQNSAWQKGRFGENTIIGNIDTGV 156

Query: 151 WPESKSFNDAGYGPVPSRWM-GVCE-----GGSNFTCNKKLIGARYFNKGFEAENGPMSA 210
           WPES+SF+D GYG VPS+W  G+C+     G    TCN+KLIGARY+NK FEA NG +  
Sbjct: 157 WPESQSFSDKGYGTVPSKWRGGLCQINKLPGSMKNTCNRKLIGARYYNKAFEAHNGQLDP 216

Query: 211 NLSTARDQEGHGSHTLSTAGGNFVPGANVFGNGNGTAKGGSPKARLAAYKVCWPSFN-GG 270
            L TARD  GHG+HTLSTAGGNFVPGA VF  GNGTAKGGSP+AR+AAYKVCW   +   
Sbjct: 217 LLHTARDFVGHGTHTLSTAGGNFVPGARVFAVGNGTAKGGSPRARVAAYKVCWSLTDPAS 276

Query: 271 CYDADILAAIEAAIHDGVDVLSISLGSS----ARDFASDTLSVGAFHAVQQGIVVVCSGG 330
           CY AD+LAAI+ AI DGVDV+++S G S    A    +D +S+GAFHA+ + I++V S G
Sbjct: 277 CYGADVLAAIDQAIDDGVDVINVSFGVSYVVTAEGIFTDEISIGAFHAISKNILLVASAG 336

Query: 331 NDGPTPGTVTNVSPWMFTVAASTIDRDFVNYVALGNKRHFEGVSLSSGGLPRGKFYPLVD 390
           NDGPTPGTV NV+PW+FT+AAST+DRDF + + + N+   EG SL    LP  + + L+ 
Sbjct: 337 NDGPTPGTVANVAPWVFTIAASTLDRDFSSNLTINNQL-IEGASLFV-NLPPNQAFSLIL 396

Query: 391 GVQVKAANATDNLALLCEDGSLDPVKAKGKIVLCLR-GDSARMDKSFEVRRAGGVGLILV 450
               K ANAT   A LC  G+LD  K  GKIVLC R G    + +  E   AG  G+IL 
Sbjct: 397 STDAKLANATFRDAQLCRRGTLDRTKVNGKIVLCTREGKIKSVAEGLEALTAGARGMILN 456

Query: 451 NDKQDGNDITADPHFLPA---------SHLNYADGLAIFQYVNSTKSPMAF-ITHVKTEL 510
           N  Q+G  ++A+PH             S  +     AI    +  K+     ++  +T  
Sbjct: 457 NQMQNGKTLSAEPHVFSTVNTPPRRAKSRPHGVKTTAIGDEDDPLKTGDTIKMSRARTLF 516

Query: 511 GIKPSPMVADFSSRGPNPIIDSMIKPDIAAPGVSILAAFSKYVTATDFPLDTRR-VPFNF 570
           G KP+P++A FSSRGPN I  S++KPD+ APGV+ILAA+S++ +A+   +D RR   FN 
Sbjct: 517 GRKPAPVMASFSSRGPNKIQPSILKPDVTAPGVNILAAYSEFASASSLLVDNRRGFKFNV 576

Query: 571 ESGTSMACPHISGVVGLLKTLYPNWSPAAIKSAIMTTAKTRDNSMKSILD-YNKVKATPF 630
             GTSM+CPH SG+ GLLKT +P+WSPAAIKSAIMTTA T DN+ + I D ++K  A  F
Sbjct: 577 LQGTSMSCPHASGIAGLLKTRHPSWSPAAIKSAIMTTATTLDNTNRPIQDAFDKTLADAF 636

Query: 631 QYGAGHVHPNNAIDPGLVYDTTIEDYMNFICAQGYNSTTLKKF-YNKPFLCPKSFALADL 690
            YG+GHV P+ AI+PGLVYD ++ DY+NF+CA GY+   +    +N+ F+C  S ++ DL
Sbjct: 637 AYGSGHVRPDLAIEPGLVYDLSLTDYLNFLCASGYDQQLISALNFNRTFICSGSHSVNDL 696

Query: 691 NYPSISVPKLTAGVPATINRRLKNVGTPGTYVARVKVSSKVSVTVKPSTLQFNSVGEEKA 750
           NYPSI++P L    P TI R + NVG P TY    +  +  S+ V P +L F  +GE K 
Sbjct: 697 NYPSITLPNLRL-KPVTIARTVTNVGPPSTYTVSTRSPNGYSIAVVPPSLTFTKIGERKT 756

Query: 751 FKVVFEYKGKGIGKGHVFGTLIWSDGKHFVRSPMAV 762
           FKV+ +       + + FG L W+DGKH VRSP+ V
Sbjct: 757 FKVIVQASSAATRRKYEFGDLRWTDGKHIVRSPITV 786

BLAST of PI0002201 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 597.4 bits (1539), Expect = 2.2e-169
Identity = 333/758 (43.93%), Postives = 472/758 (62.27%), Query Frame = 0

Query: 25  ILHCLFGSHF--FGSNPSIYDVQLATE---SQYELLGSVVGSKLAA---KESIFYSYNRY 84
           +L CL   H     S+   Y V +A     S ++L  +   S L +      + Y+Y   
Sbjct: 13  LLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAELLYTYENA 72

Query: 85  INGFAAILDENQANALARNPNVVSIFENQKRKLHTTRSWSFLGVENDEGIPPNSIWKAAR 144
           I+GF+  L + +A++L   P V+S+    + +LHTTR+  FLG++         ++  A 
Sbjct: 73  IHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTA----DLFPEAG 132

Query: 145 FGEDTIIANLDTGAWPESKSFNDAGYGPVPSRWMGVCEGGSNFT---CNKKLIGARYFNK 204
              D ++  LDTG WPESKS++D G+GP+PS W G CE G+NFT   CN+KLIGAR+F +
Sbjct: 133 SYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFAR 192

Query: 205 GFEAENGPM--SANLSTARDQEGHGSHTLSTAGGNFVPGANVFGNGNGTAKGGSPKARLA 264
           G+E+  GP+  S    + RD +GHG+HT STA G+ V GA++ G  +GTA+G +P+AR+A
Sbjct: 193 GYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVA 252

Query: 265 AYKVCWPSFNGGCYDADILAAIEAAIHDGVDVLSISLGSSARDFASDTLSVGAFHAVQQG 324
            YKVCW    GGC+ +DILAAI+ AI D V+VLS+SLG    D+  D +++GAF A+++G
Sbjct: 253 VYKVCW---LGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERG 312

Query: 325 IVVVCSGGNDGPTPGTVTNVSPWMFTVAASTIDRDFVNYVALGNKRHFEGVSLSSGGLPR 384
           I+V CS GN GP+  +++NV+PW+ TV A T+DRDF     LGN ++F GVSL  G    
Sbjct: 313 ILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALP 372

Query: 385 GKFYPLVDGVQVKAANATDNLALLCEDGSLDPVKAKGKIVLCLRGDSARMDKSFEVRRAG 444
            K  P +      A+NAT+    LC  G+L P K KGKIV+C RG +AR+ K   V+ AG
Sbjct: 373 DKLLPFI--YAGNASNATN--GNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAG 432

Query: 445 GVGLILVNDKQDGNDITADPHFLPASHLNYADGLAIFQYVNSTKSPMAFITHVKTELGIK 504
           GVG+IL N   +G ++ AD H LPA+ +    G  I  YV +  +P A I+ + T +G+K
Sbjct: 433 GVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVK 492

Query: 505 PSPMVADFSSRGPNPIIDSMIKPDIAAPGVSILAAFSKYVTATDFPLDTRRVPFNFESGT 564
           PSP+VA FSSRGPN I  +++KPD+ APGV+ILAA++     T    D+RRV FN  SGT
Sbjct: 493 PSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGT 552

Query: 565 SMACPHISGVVGLLKTLYPNWSPAAIKSAIMTTAKTRDNSMKSILDYNKVK-ATPFQYGA 624
           SM+CPH+SG+  LLK+++P WSPAAI+SA+MTTA       K +LD    K +TPF +GA
Sbjct: 553 SMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGA 612

Query: 625 GHVHPNNAIDPGLVYDTTIEDYMNFICAQGYNSTTLKKFYNKPFLC--PKSFALADLNYP 684
           GHV P  A +PGL+YD T EDY+ F+CA  Y S  ++    + + C   KS+++ADLNYP
Sbjct: 613 GHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYP 672

Query: 685 SISVPKLTAGVPA-TINRRLKNVGTPGTYVARVKVSSK---VSVTVKPSTLQFNSVGEEK 744
           S +V     GV A    R + +VG  GTY   VKV+S+   V ++V+P+ L F    E+K
Sbjct: 673 SFAVN--VDGVGAYKYTRTVTSVGGAGTY--SVKVTSETTGVKISVEPAVLNFKEANEKK 732

Query: 745 AFKVVFEYKGKGIGKGHVFGTLIWSDGKHFVRSPMAVA 763
           ++ V F          + FG++ WSDGKH V SP+A++
Sbjct: 733 SYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

BLAST of PI0002201 vs. ExPASy Swiss-Prot
Match: Q9LVJ1 (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 SV=1)

HSP 1 Score: 564.3 bits (1453), Expect = 2.1e-159
Identity = 312/717 (43.51%), Postives = 443/717 (61.79%), Query Frame = 0

Query: 68  SIFYSYNRYINGFAAILDENQANALARNPNVVSIFENQKRKLHTTRSWSFLGVENDEGIP 127
           ++ YSY+R ++GF+A L   Q  AL R+P+V+S+  +Q R++HTT + +FLG   + G  
Sbjct: 68  TLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSG-- 127

Query: 128 PNSIWKAARFGEDTIIANLDTGAWPESKSFNDAGYGPVPSRWMGVCEGGSNF---TCNKK 187
              +W  + +GED I+  LDTG WPE  SF+D+G GP+PS W G CE G +F   +CN+K
Sbjct: 128 ---LWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRK 187

Query: 188 LIGARYFNKGFEAENGPMSANLS----TARDQEGHGSHTLSTAGGNFVPGANVFGNGNGT 247
           LIGAR F +G+  +      + +    + RD EGHG+HT STA G+ V  A+++    GT
Sbjct: 188 LIGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGT 247

Query: 248 AKGGSPKARLAAYKVCWPSFNGGCYDADILAAIEAAIHDGVDVLSISLGS--SARDFASD 307
           A G + KAR+AAYK+CW    GGCYD+DILAA++ A+ DGV V+S+S+G+  SA ++ +D
Sbjct: 248 ATGMASKARIAAYKICW---TGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTD 307

Query: 308 TLSVGAFHAVQQGIVVVCSGGNDGPTPGTVTNVSPWMFTVAASTIDRDFVNYVALGNKRH 367
           ++++GAF A + GIVV CS GN GP P T TN++PW+ TV AST+DR+F      G+ + 
Sbjct: 308 SIAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKV 367

Query: 368 FEGVSLSSG-GLPRGKFYPLVDGVQVKAANATDNLALLCEDGSLDPVKAKGKIVLCLRGD 427
           F G SL +G  LP           Q+    + D  + LC  G L+    +GKIVLC RG 
Sbjct: 368 FTGTSLYAGESLPDS---------QLSLVYSGDCGSRLCYPGKLNSSLVEGKIVLCDRGG 427

Query: 428 SARMDKSFEVRRAGGVGLILVNDKQDGNDITADPHFLPASHLNYADGLAIFQYVNSTKSP 487
           +AR++K   V+ AGG G+IL N  + G ++TAD H +PA+ +    G  I  Y+ ++ SP
Sbjct: 428 NARVEKGSAVKLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSP 487

Query: 488 MAFITHVKTELG-IKPSPMVADFSSRGPNPIIDSMIKPDIAAPGVSILAAFSKYVTATDF 547
            A I+ + T +G   PSP VA FSSRGPN +   ++KPD+ APGV+ILA ++  V  TD 
Sbjct: 488 TAKISFLGTLIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDL 547

Query: 548 PLDTRRVPFNFESGTSMACPHISGVVGLLKTLYPNWSPAAIKSAIMTTAKTRDNSMKSIL 607
            +D RRV FN  SGTSM+CPH+SG+  LL+  +P+WSPAAIKSA++TTA   +NS + I 
Sbjct: 548 DIDPRRVQFNIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIE 607

Query: 608 DYNKVKAT-PFQYGAGHVHPNNAIDPGLVYDTTIEDYMNFICAQGYNSTTLKKFYNKPFL 667
           D    K++  F +GAGHV PN A++PGLVYD  +++Y+ F+CA GY    +  F   P L
Sbjct: 608 DLATGKSSNSFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTL 667

Query: 668 -----CPKSFALADLNYPSISVPKLTAGVPATINRRLKNVGT--PGTYVARVKVSSKVSV 727
                  K     DLNYPS SV   + G      R +KNVG+     Y   VK  + V +
Sbjct: 668 YDACETSKLRTAGDLNYPSFSVVFASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEI 727

Query: 728 TVKPSTLQFNSVGEEKAFKVVFE--YKGKGIGK--GHVFGTLIWSDGKHFVRSPMAV 762
            V PS L F+       ++V F+    G G+G   GH FG++ W+DG+H V+SP+AV
Sbjct: 728 DVSPSKLAFSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAV 767

BLAST of PI0002201 vs. ExPASy TrEMBL
Match: A0A5D3D732 (Subtilisin-like protease SBT5.3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G00170 PE=3 SV=1)

HSP 1 Score: 1426.8 bits (3692), Expect = 0.0e+00
Identity = 699/744 (93.95%), Postives = 720/744 (96.77%), Query Frame = 0

Query: 20   KQKNTILHCLFGSHFFGSNPSIYDVQLATESQYELLGSVVGSKLAAKESIFYSYNRYING 79
            KQ + I++   GSHFFGSNPSI+DVQLATESQYELL SVVGSKLAAKESIFYSYNRYING
Sbjct: 740  KQNSYIVY--LGSHFFGSNPSIHDVQLATESQYELLESVVGSKLAAKESIFYSYNRYING 799

Query: 80   FAAILDENQANALARNPNVVSIFENQKRKLHTTRSWSFLGVENDEGIPPNSIWKAARFGE 139
            FAAILDENQA ALARNPNVVSIFENQKRKLHTTRSWSFLG+E+DEGIPPNSIWKAARFGE
Sbjct: 800  FAAILDENQAIALARNPNVVSIFENQKRKLHTTRSWSFLGMESDEGIPPNSIWKAARFGE 859

Query: 140  DTIIANLDTGAWPESKSFNDAGYGPVPSRWMGVCEGGSNFTCNKKLIGARYFNKGFEAEN 199
            DTII NLDTGAWPESKSFNDAGYGPVPSRWMGVCEGG+NFTCNKKLIGARYFNKGFEAEN
Sbjct: 860  DTIIGNLDTGAWPESKSFNDAGYGPVPSRWMGVCEGGANFTCNKKLIGARYFNKGFEAEN 919

Query: 200  GPMSANLSTARDQEGHGSHTLSTAGGNFVPGANVFGNGNGTAKGGSPKARLAAYKVCWPS 259
            GPMSANL+TARDQEGHGSHTLSTAGGNFVPGANVFGNGNGTAKGGSP+ARLAAYKVCWPS
Sbjct: 920  GPMSANLTTARDQEGHGSHTLSTAGGNFVPGANVFGNGNGTAKGGSPRARLAAYKVCWPS 979

Query: 260  FNGGCYDADILAAIEAAIHDGVDVLSISLGSSARDFASDTLSVGAFHAVQQGIVVVCSGG 319
            F GGCYDADILAA+E+AIHDGVDVLSISLGSSARDFASDTLS+GAFHAVQQGIVVVCSGG
Sbjct: 980  FTGGCYDADILAAVESAIHDGVDVLSISLGSSARDFASDTLSIGAFHAVQQGIVVVCSGG 1039

Query: 320  NDGPTPGTVTNVSPWMFTVAASTIDRDFVNYVALGNKRHFEGVSLSSGGLPRGKFYPLVD 379
            NDGPTPGTVTNVSPWM TVAAST+DRDFVNYVALGNKRHF+GVSLSSGGLPRGKFYPLVD
Sbjct: 1040 NDGPTPGTVTNVSPWMITVAASTVDRDFVNYVALGNKRHFKGVSLSSGGLPRGKFYPLVD 1099

Query: 380  GVQVKAANATDNLALLCEDGSLDPVKAKGKIVLCLRGDSARMDKSFEVRRAGGVGLILVN 439
            GVQVKA NATD LALLCEDGSLDP KAKGKIVLCLRGDSARMDKSFEVRRAGG+GLILVN
Sbjct: 1100 GVQVKAGNATDKLALLCEDGSLDPAKAKGKIVLCLRGDSARMDKSFEVRRAGGIGLILVN 1159

Query: 440  DKQDGNDITADPHFLPASHLNYADGLAIFQYVNSTKSPMAFITHVKTELGIKPSPMVADF 499
            DK+DGNDITADPHFLPASHLNYADG+AIFQY+NSTKSPMAFITHVKTELGIKPSPMVADF
Sbjct: 1160 DKEDGNDITADPHFLPASHLNYADGIAIFQYINSTKSPMAFITHVKTELGIKPSPMVADF 1219

Query: 500  SSRGPNPIIDSMIKPDIAAPGVSILAAFSKYVTATDFPLDTRRVPFNFESGTSMACPHIS 559
            SSRGPNPIIDSMIKPDIAAPGVSILAAFS+Y TATDFPLDTRRV FNFESGTSMACPHIS
Sbjct: 1220 SSRGPNPIIDSMIKPDIAAPGVSILAAFSEYATATDFPLDTRRVSFNFESGTSMACPHIS 1279

Query: 560  GVVGLLKTLYPNWSPAAIKSAIMTTAKTRDNSMKSILDYNKVKATPFQYGAGHVHPNNAI 619
            GVVGLLKTLYP WSPAAIKSAIMTTAKTRDNSMKSILDYNK KATPFQYGAGHVHPNNAI
Sbjct: 1280 GVVGLLKTLYPKWSPAAIKSAIMTTAKTRDNSMKSILDYNKAKATPFQYGAGHVHPNNAI 1339

Query: 620  DPGLVYDTTIEDYMNFICAQGYNSTTLKKFYNKPFLCPKSFALADLNYPSISVPKLTAGV 679
            DPGLVYDTTIEDYMNFICAQGYNSTTLK+FYNKP+LCPKSF L DLNYPSISVPKLT GV
Sbjct: 1340 DPGLVYDTTIEDYMNFICAQGYNSTTLKRFYNKPYLCPKSFPLTDLNYPSISVPKLTIGV 1399

Query: 680  PATINRRLKNVGTPGTYVARVKVSSKVSVTVKPSTLQFNSVGEEKAFKVVFEYKGKGIGK 739
            P TINRRLKNVGTPGTYVARVKVSSKVSVTVKPSTLQFNSVGEEKAFKVVFEYKGKG GK
Sbjct: 1400 PVTINRRLKNVGTPGTYVARVKVSSKVSVTVKPSTLQFNSVGEEKAFKVVFEYKGKGQGK 1459

Query: 740  GHVFGTLIWSDGKHFVRSPMAVAL 764
            GHVFGTLIWSDGKHFVRSPM V L
Sbjct: 1460 GHVFGTLIWSDGKHFVRSPMVVKL 1481

BLAST of PI0002201 vs. ExPASy TrEMBL
Match: A0A1S3BLG8 (subtilisin-like protease SBT5.3 OS=Cucumis melo OX=3656 GN=LOC103491134 PE=3 SV=1)

HSP 1 Score: 1424.1 bits (3685), Expect = 0.0e+00
Identity = 695/733 (94.82%), Postives = 714/733 (97.41%), Query Frame = 0

Query: 31   GSHFFGSNPSIYDVQLATESQYELLGSVVGSKLAAKESIFYSYNRYINGFAAILDENQAN 90
            GSHFFGSNPSI+DVQLATESQYELL SVVGSKLAAKESIFYSYNRYINGFAAILDENQA 
Sbjct: 778  GSHFFGSNPSIHDVQLATESQYELLESVVGSKLAAKESIFYSYNRYINGFAAILDENQAI 837

Query: 91   ALARNPNVVSIFENQKRKLHTTRSWSFLGVENDEGIPPNSIWKAARFGEDTIIANLDTGA 150
            ALARNPNVVSIFENQKRKLHTTRSWSFLG+E+DEGIPPNSIWKAARFGEDTII NLDTGA
Sbjct: 838  ALARNPNVVSIFENQKRKLHTTRSWSFLGMESDEGIPPNSIWKAARFGEDTIIGNLDTGA 897

Query: 151  WPESKSFNDAGYGPVPSRWMGVCEGGSNFTCNKKLIGARYFNKGFEAENGPMSANLSTAR 210
            WPESKSFNDAGYGPVPSRWMGVCEGG+NFTCNKKLIGARYFNKGFEAENGPMSANL+TAR
Sbjct: 898  WPESKSFNDAGYGPVPSRWMGVCEGGANFTCNKKLIGARYFNKGFEAENGPMSANLTTAR 957

Query: 211  DQEGHGSHTLSTAGGNFVPGANVFGNGNGTAKGGSPKARLAAYKVCWPSFNGGCYDADIL 270
            DQEGHGSHTLSTAGGNFVPGANVFGNGNGTAKGGSP+ARLAAYKVCWPSF GGCYDADIL
Sbjct: 958  DQEGHGSHTLSTAGGNFVPGANVFGNGNGTAKGGSPRARLAAYKVCWPSFTGGCYDADIL 1017

Query: 271  AAIEAAIHDGVDVLSISLGSSARDFASDTLSVGAFHAVQQGIVVVCSGGNDGPTPGTVTN 330
            AA+E+AIHDGVDVLSISLGSSARDFASDTLS+GAFHAVQQGIVVVCSGGNDGPTPGTVTN
Sbjct: 1018 AAVESAIHDGVDVLSISLGSSARDFASDTLSIGAFHAVQQGIVVVCSGGNDGPTPGTVTN 1077

Query: 331  VSPWMFTVAASTIDRDFVNYVALGNKRHFEGVSLSSGGLPRGKFYPLVDGVQVKAANATD 390
            VSPWM TVAAST+DRDFVNYVALGNKRHF+GVSLSSGGLPRGKFYPLVDGVQVKA NATD
Sbjct: 1078 VSPWMITVAASTVDRDFVNYVALGNKRHFKGVSLSSGGLPRGKFYPLVDGVQVKAGNATD 1137

Query: 391  NLALLCEDGSLDPVKAKGKIVLCLRGDSARMDKSFEVRRAGGVGLILVNDKQDGNDITAD 450
             LALLCEDGSLDP KAKGKIVLCLRGDSARMDKSFEVRRAGG+GLILVNDK+DGNDITAD
Sbjct: 1138 KLALLCEDGSLDPAKAKGKIVLCLRGDSARMDKSFEVRRAGGIGLILVNDKEDGNDITAD 1197

Query: 451  PHFLPASHLNYADGLAIFQYVNSTKSPMAFITHVKTELGIKPSPMVADFSSRGPNPIIDS 510
            PHFLPASHLNYADG+AIFQY+NSTKSPMAFITHVKTE+GIKPSPMVADFSSRGPNPIIDS
Sbjct: 1198 PHFLPASHLNYADGIAIFQYINSTKSPMAFITHVKTEMGIKPSPMVADFSSRGPNPIIDS 1257

Query: 511  MIKPDIAAPGVSILAAFSKYVTATDFPLDTRRVPFNFESGTSMACPHISGVVGLLKTLYP 570
            MIKPDIAAPGVSILAAFS+Y TATDFPLDTRRV FNFESGTSMACPHISGVVGLLKTLYP
Sbjct: 1258 MIKPDIAAPGVSILAAFSEYATATDFPLDTRRVSFNFESGTSMACPHISGVVGLLKTLYP 1317

Query: 571  NWSPAAIKSAIMTTAKTRDNSMKSILDYNKVKATPFQYGAGHVHPNNAIDPGLVYDTTIE 630
             WSPAAIKSAIMTTAKTRDNSMKSILDYNK KATPFQYGAGHVHPNNAIDPGLVYDTTIE
Sbjct: 1318 KWSPAAIKSAIMTTAKTRDNSMKSILDYNKAKATPFQYGAGHVHPNNAIDPGLVYDTTIE 1377

Query: 631  DYMNFICAQGYNSTTLKKFYNKPFLCPKSFALADLNYPSISVPKLTAGVPATINRRLKNV 690
            DYMNFICAQGYNSTTLK+FYNKP+LCPKSF L DLNYPSISVPKLT GVP TINRRLKNV
Sbjct: 1378 DYMNFICAQGYNSTTLKRFYNKPYLCPKSFPLTDLNYPSISVPKLTIGVPVTINRRLKNV 1437

Query: 691  GTPGTYVARVKVSSKVSVTVKPSTLQFNSVGEEKAFKVVFEYKGKGIGKGHVFGTLIWSD 750
            GTPGTYVARVKVSSKVSVTVKPSTLQFNSVGEEKAFKVVFEYKGKG GKGHVFGTLIWSD
Sbjct: 1438 GTPGTYVARVKVSSKVSVTVKPSTLQFNSVGEEKAFKVVFEYKGKGQGKGHVFGTLIWSD 1497

Query: 751  GKHFVRSPMAVAL 764
            G HFVRSPMAV L
Sbjct: 1498 GNHFVRSPMAVKL 1510

BLAST of PI0002201 vs. ExPASy TrEMBL
Match: A0A0A0L601 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G045060 PE=3 SV=1)

HSP 1 Score: 1144.8 bits (2960), Expect = 0.0e+00
Identity = 547/734 (74.52%), Postives = 634/734 (86.38%), Query Frame = 0

Query: 31  GSHFFGSNPSIYDVQLATESQYELLGSVVGSKLAAKESIFYSYNRYINGFAAILDENQAN 90
           G+   GSNP+ YD+++ATESQY+LLGSVVGSKLAAK++I YSYN+YINGFAA LDE QA 
Sbjct: 12  GAPSVGSNPTNYDIEVATESQYDLLGSVVGSKLAAKDAIKYSYNKYINGFAATLDEKQAK 71

Query: 91  ALARNPNVVSIFENQKRKLHTTRSWSFLGVENDEGIPPNSIWKAARFGEDTIIANLDTGA 150
            LA+NP VVS+FEN++RKLHTTRSW FLGVE+DEGIP NSIW A RFGEDTII NLDTG 
Sbjct: 72  DLAKNPKVVSVFENKERKLHTTRSWHFLGVESDEGIPSNSIWNAGRFGEDTIIGNLDTGV 131

Query: 151 WPESKSFNDAGYGPVPSRWMGVCEGGSNFTCNKKLIGARYFNKGFEAENGPMSANLSTAR 210
           WPESKSFNDAGYGPVPSRW G CEGG+NF CN+KLIGARYFNKGF   +GP++ + +TAR
Sbjct: 132 WPESKSFNDAGYGPVPSRWRGACEGGANFRCNRKLIGARYFNKGFAMASGPLNISFNTAR 191

Query: 211 DQEGHGSHTLSTAGGNFVPGANVFGNGNGTAKGGSPKARLAAYKVCWPSFN-GGCYDADI 270
           D++GHGSHTLSTAGGNFVPGANVFG GNGTAKGGSPKAR+AAYKVCWP+ + GGCYDADI
Sbjct: 192 DKQGHGSHTLSTAGGNFVPGANVFGYGNGTAKGGSPKARVAAYKVCWPATSGGGCYDADI 251

Query: 271 LAAIEAAIHDGVDVLSISLGSSARDFASDTLSVGAFHAVQQGIVVVCSGGNDGPTPGTVT 330
           LA  EAAI DGVDVLS+SLGS   +FA D++S+GAFHAVQQGIVVVCS GNDGP PGTV+
Sbjct: 252 LAGFEAAISDGVDVLSVSLGSKPEEFAYDSMSIGAFHAVQQGIVVVCSAGNDGPGPGTVS 311

Query: 331 NVSPWMFTVAASTIDRDFVNYVALGNKRHFEGVSLSSGGLPRGKFYPLVDGVQVKAANAT 390
           N+SPWMFTVAAS+IDRDF +Y +LGNK+H++G S+SS  L  GKFYPL++ V  KAANA+
Sbjct: 312 NISPWMFTVAASSIDRDFTSYASLGNKKHYKGSSISSSALAGGKFYPLINAVDAKAANAS 371

Query: 391 DNLALLCEDGSLDPVKAKGKIVLCLRGDSARMDKSFEVRRAGGVGLILVNDKQDGNDITA 450
           + LA LC  GSLDP KAKGKI++CLRG++AR++K F V +AGGVG+ILVN K  G+  TA
Sbjct: 372 EILAQLCHKGSLDPTKAKGKIIVCLRGENARVEKGFVVLQAGGVGMILVNGKNGGSGTTA 431

Query: 451 DPHFLPASHLNYADGLAIFQYVNSTKSPMAFITHVKTELGIKPSPMVADFSSRGPNPIID 510
           D H LPA+HL+Y DGLA+ QY+NSTK+P+A IT V+T+LGIKPSP++ADFSSRGPNPI +
Sbjct: 432 DAHILPATHLSYTDGLAVAQYINSTKTPVAHITPVQTQLGIKPSPVMADFSSRGPNPITE 491

Query: 511 SMIKPDIAAPGVSILAAFSKYVTATDFPLDTRRVPFNFESGTSMACPHISGVVGLLKTLY 570
           +M+KPDI  PG+SILA+ +  VTAT FP DTRRVPFN ESGTSM+CPHISGVVGLLKTLY
Sbjct: 492 AMLKPDITGPGMSILASVTTDVTATTFPFDTRRVPFNVESGTSMSCPHISGVVGLLKTLY 551

Query: 571 PNWSPAAIKSAIMTTAKTRDNSMKSILDYNKVKATPFQYGAGHVHPNNAIDPGLVYDTTI 630
           P WSPAAIKSAIMTTAKTRDN+M++I D  K KATPF YGAGHVHPN+A+DPGLVYDTTI
Sbjct: 552 PTWSPAAIKSAIMTTAKTRDNTMRTISDNVKPKATPFDYGAGHVHPNSAMDPGLVYDTTI 611

Query: 631 EDYMNFICAQGYNSTTLKKFYNKPFLCPKSFALADLNYPSISVPKLTAGVPATINRRLKN 690
           +DY+NF+CA+GYNS T K FYNKPF+C KSF L DLNYPSIS+PKL  G P T+NRR+KN
Sbjct: 612 DDYLNFLCARGYNSLTFKNFYNKPFVCAKSFTLTDLNYPSISIPKLQFGAPVTVNRRVKN 671

Query: 691 VGTPGTYVARVKVSSKVSVTVKPSTLQFNSVGEEKAFKVVFEYKGKGIGKGHVFGTLIWS 750
           VGTPGTYVARV  SSK+ VTV+PSTLQFNSVGEEKAFKVVFEYKG    KG+VFGTLIWS
Sbjct: 672 VGTPGTYVARVNASSKILVTVEPSTLQFNSVGEEKAFKVVFEYKGNEQDKGYVFGTLIWS 731

Query: 751 DGKHFVRSPMAVAL 764
           DGKH VRSP+ V L
Sbjct: 732 DGKHNVRSPIVVNL 745

BLAST of PI0002201 vs. ExPASy TrEMBL
Match: A0A6J1GDR2 (subtilisin-like protease SBT5.3 OS=Cucurbita moschata OX=3662 GN=LOC111453063 PE=3 SV=1)

HSP 1 Score: 1110.5 bits (2871), Expect = 0.0e+00
Identity = 529/743 (71.20%), Postives = 631/743 (84.93%), Query Frame = 0

Query: 21  QKNTILHCLFGSHFFGSNPSIYDVQLATESQYELLGSVVGSKLAAKESIFYSYNRYINGF 80
           +K+ I++   GS  FGSNPSIYDVQLATES+Y++LG+V GSK+AAKESI Y+YNR INGF
Sbjct: 25  KKSYIVYLGSGSSTFGSNPSIYDVQLATESKYDILGTVKGSKMAAKESILYTYNRCINGF 84

Query: 81  AAILDENQANALARNPNVVSIFENQKRKLHTTRSWSFLGVENDEGIPPNSIWKAARFGED 140
           AA+LD+N+  ALA+NP+VVS+FEN++RKLHTTRSW FLGV++  GIP NSIWKA++FGED
Sbjct: 85  AAVLDDNEVTALAKNPSVVSVFENRERKLHTTRSWGFLGVDSGRGIPQNSIWKASKFGED 144

Query: 141 TIIANLDTGAWPESKSFNDAGYGPVPSRWMGVCEGGSNFTCNKKLIGARYFNKGFEAENG 200
            II NLDTG WPES+SF+DAGYGPVPSRWMG CEGGS F CN+KLIGARYF +G+E  NG
Sbjct: 145 VIIGNLDTGVWPESESFHDAGYGPVPSRWMGACEGGSKFRCNRKLIGARYFYRGYEMING 204

Query: 201 PMSANLSTARDQEGHGSHTLSTAGGNFVPGANVFGNGNGTAKGGSPKARLAAYKVCWPSF 260
           P++ +   ARD EGHG+HTLSTAGGNFV GANVFGNGNGTAKGG+PKAR+AAYKVCWP  
Sbjct: 205 PLNISSLNARDHEGHGTHTLSTAGGNFVQGANVFGNGNGTAKGGAPKARVAAYKVCWP-- 264

Query: 261 NGGCYDADILAAIEAAIHDGVDVLSISLGSSARDFASDTLSVGAFHAVQQGIVVVCSGGN 320
            G C DAD+LA IEAAI DGVDVLSISLG++A+DFA+D +SVGAFHA+QQGI+VVCS GN
Sbjct: 265 QGKCSDADLLAGIEAAISDGVDVLSISLGATAQDFANDPISVGAFHAIQQGIIVVCSAGN 324

Query: 321 DGPTPGTVTNVSPWMFTVAASTIDRDFVNYVALGNKRHFEGVSLSSGGLPRGKFYPLVDG 380
           DGP PGTVTNVSPWMFTV AS+IDR F +YV LGNK+  +G SLSSGGLPRGK YPL++ 
Sbjct: 325 DGPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQIKGSSLSSGGLPRGKLYPLMNS 384

Query: 381 VQVKAANATDNLALLCEDGSLDPVKAKGKIVLCLRGDSARMDKSFEVRRAGGVGLILVND 440
           V  KA+NA+D LA LCE+GSLDP+KA+GKI++CLRGD+ RMDKSFEV R GGVG+ILVND
Sbjct: 385 VNAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDNGRMDKSFEVLRVGGVGMILVND 444

Query: 441 KQDGNDITADPHFLPASHLNYADGLAIFQYVNSTKSPMAFITHVKTELGIKPSPMVADFS 500
           K  G+ I  DPH LP SH++Y DGL+I QY+ STK P+A IT V+TE+GIKPSP++A FS
Sbjct: 445 KISGSGIETDPHMLPTSHVSYIDGLSIAQYLKSTKRPVATITPVRTEIGIKPSPVMATFS 504

Query: 501 SRGPNPIIDSMIKPDIAAPGVSILAAFSKYVTATDFPLDTRRVPFNFESGTSMACPHISG 560
           SRGPN I +++IKPDI APGV+I+A+F++   ATD P D RRVPFN +SGTSM+CPHISG
Sbjct: 505 SRGPNHITEAIIKPDITAPGVNIIASFTRAAAATDLPFDKRRVPFNVQSGTSMSCPHISG 564

Query: 561 VVGLLKTLYPNWSPAAIKSAIMTTAKTRDNSMKSILDYNKVKATPFQYGAGHVHPNNAID 620
           V GLLK L+P WSPAAIKSAIMTTAKTRDN+  ++LD+NKVKATPF YGAG VHPNNA+D
Sbjct: 565 VAGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDFNKVKATPFDYGAGLVHPNNAMD 624

Query: 621 PGLVYDTTIEDYMNFICAQGYNSTTLKKFYNKPFLCPKSFALADLNYPSISVPKLTAGVP 680
           PGLVYDTTI+DY+NF+C QGYNS TLKKF NKPF+C K+FA+ DLNYPSISVPKL  G P
Sbjct: 625 PGLVYDTTIDDYLNFLCTQGYNSLTLKKFSNKPFVCSKNFAITDLNYPSISVPKLQIGAP 684

Query: 681 ATINRRLKNVGTPGTYVARVKVSSKVSVTVKPSTLQFNSVGEEKAFKVVFEYKGKGIGKG 740
            T+NRR+KNVG+ GTYVARV++   ++V V+PSTLQF+ VGEEK FK+VF Y  +    G
Sbjct: 685 VTVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFHGVGEEKPFKLVFHYAQQVRRPG 744

Query: 741 HVFGTLIWSDGKHFVRSPMAVAL 764
           +VFG L+WSDGKHFVRSP+AV L
Sbjct: 745 YVFGALVWSDGKHFVRSPIAVNL 765

BLAST of PI0002201 vs. ExPASy TrEMBL
Match: A0A6J1KUL8 (uncharacterized protein LOC111498820 OS=Cucurbita maxima OX=3661 GN=LOC111498820 PE=3 SV=1)

HSP 1 Score: 1109.0 bits (2867), Expect = 0.0e+00
Identity = 530/727 (72.90%), Postives = 626/727 (86.11%), Query Frame = 0

Query: 37  SNPSIYDVQLATESQYELLGSVVGSKLAAKESIFYSYNRYINGFAAILDENQANALARNP 96
           SNPSIYDVQLATES+Y++LG+V GSK+AAKESI Y+YNR INGFAA+LD+ +  ALA+NP
Sbjct: 36  SNPSIYDVQLATESKYDILGTVKGSKVAAKESILYTYNRCINGFAAVLDDKEVTALAKNP 95

Query: 97  NVVSIFENQKRKLHTTRSWSFLGVENDEGIPPNSIWKAARFGEDTIIANLDTGAWPESKS 156
           +VVSIFEN++RKLHTTRSWSFLGV +D GIP NSIWKA++FGED II NLDTG WPES S
Sbjct: 96  SVVSIFENRERKLHTTRSWSFLGVGSDRGIPWNSIWKASKFGEDVIIGNLDTGVWPESHS 155

Query: 157 FNDAGYGPVPSRWMGVCEGGSNFTCNKKLIGARYFNKGFEAENGPMSANLSTARDQEGHG 216
           F+D+GYGPVPSRWMG CEGGSNF+CN+KLIGARYF +G+E  NGP++ +   ARD EGHG
Sbjct: 156 FHDSGYGPVPSRWMGACEGGSNFSCNRKLIGARYFYRGYEMINGPLNISSLNARDHEGHG 215

Query: 217 SHTLSTAGGNFVPGANVFGNGNGTAKGGSPKARLAAYKVCWPSFNGGCYDADILAAIEAA 276
           +HTLSTAGGNFV GANVFGNGNGTAKGG+PKAR+AAYKVCWP   G C DAD+LA IEAA
Sbjct: 216 THTLSTAGGNFVRGANVFGNGNGTAKGGAPKARVAAYKVCWP--QGKCSDADLLAGIEAA 275

Query: 277 IHDGVDVLSISLGSSARDFASDTLSVGAFHAVQQGIVVVCSGGNDGPTPGTVTNVSPWMF 336
           I DGVDVLSISLG++A+DFA D +SVGAFHA+QQGI+VVCS GNDGP PGTVTNVSPWMF
Sbjct: 276 ISDGVDVLSISLGAAAQDFADDPISVGAFHAIQQGIIVVCSAGNDGPLPGTVTNVSPWMF 335

Query: 337 TVAASTIDRDFVNYVALGNKRHFEGVSLSSGGLPRGKFYPLVDGVQVKAANATDNLALLC 396
           TV AS+IDR F +YV LGNK+  +G SLSSGGLPRGK YPL++ V  KA+NA+D LA LC
Sbjct: 336 TVGASSIDRGFFSYVYLGNKKQVKGSSLSSGGLPRGKLYPLMNSVNAKASNASDGLAQLC 395

Query: 397 EDGSLDPVKAKGKIVLCLRGDSARMDKSFEVRRAGGVGLILVNDKQDGNDITADPHFLPA 456
           E+GSLDP+KA+GKI++CLRGD+ RMDKSFEV R GGVG+ILVNDK  G+DI  D H LP 
Sbjct: 396 EEGSLDPMKARGKIIVCLRGDNGRMDKSFEVLRVGGVGMILVNDKISGSDIETDAHMLPT 455

Query: 457 SHLNYADGLAIFQYVNSTKSPMAFITHVKTELGIKPSPMVADFSSRGPNPIIDSMIKPDI 516
           SH++Y DGL+I +Y+ STK P+A IT V+TE+GIKPSP++A FSSRGPN I ++MIKPDI
Sbjct: 456 SHVSYIDGLSIAEYLKSTKRPVASITPVRTEIGIKPSPVMAPFSSRGPNHIAEAMIKPDI 515

Query: 517 AAPGVSILAAFSKYVTATDFPLDTRRVPFNFESGTSMACPHISGVVGLLKTLYPNWSPAA 576
           +APGV+I+A+F+K V ATD P D RRVPFN +SGTSM+CPHI+GV GLLK L+P WSPAA
Sbjct: 516 SAPGVNIIASFTKAVAATDLPFDKRRVPFNVQSGTSMSCPHIAGVAGLLKKLHPTWSPAA 575

Query: 577 IKSAIMTTAKTRDNSMKSILDYNKVKATPFQYGAGHVHPNNAIDPGLVYDTTIEDYMNFI 636
           IKSAIMTTAKTRDN+  ++LD+NKVKATPF YGAGHVHPN+A+DPGLVYDTTI+DY+NF+
Sbjct: 576 IKSAIMTTAKTRDNTKNTMLDFNKVKATPFDYGAGHVHPNDAMDPGLVYDTTIDDYLNFL 635

Query: 637 CAQGYNSTTLKKFYNKPFLCPKSFALADLNYPSISVPKLTAGVPATINRRLKNVGTPGTY 696
           C QGYNS TLKKF NKPF+C K+FA  DLNYPSISVPKL  GVP T+NRR+KNVG+ GTY
Sbjct: 636 CMQGYNSLTLKKFSNKPFVCAKNFASTDLNYPSISVPKLQIGVPVTVNRRVKNVGSAGTY 695

Query: 697 VARVKVSSKVSVTVKPSTLQFNSVGEEKAFKVVFEYKGKGIGKGHVFGTLIWSDGKHFVR 756
           VARV++   ++V V+PSTLQF+SVGEEKAFK+VF Y  K    G+VFG L+WSDGKHFVR
Sbjct: 696 VARVRMPKGITVMVEPSTLQFHSVGEEKAFKLVFHYAQKVRRPGYVFGALVWSDGKHFVR 755

Query: 757 SPMAVAL 764
           SP+AV L
Sbjct: 756 SPIAVNL 760

BLAST of PI0002201 vs. NCBI nr
Match: TYK19386.1 (subtilisin-like protease SBT5.3 [Cucumis melo var. makuwa])

HSP 1 Score: 1426.8 bits (3692), Expect = 0.0e+00
Identity = 699/744 (93.95%), Postives = 720/744 (96.77%), Query Frame = 0

Query: 20   KQKNTILHCLFGSHFFGSNPSIYDVQLATESQYELLGSVVGSKLAAKESIFYSYNRYING 79
            KQ + I++   GSHFFGSNPSI+DVQLATESQYELL SVVGSKLAAKESIFYSYNRYING
Sbjct: 740  KQNSYIVY--LGSHFFGSNPSIHDVQLATESQYELLESVVGSKLAAKESIFYSYNRYING 799

Query: 80   FAAILDENQANALARNPNVVSIFENQKRKLHTTRSWSFLGVENDEGIPPNSIWKAARFGE 139
            FAAILDENQA ALARNPNVVSIFENQKRKLHTTRSWSFLG+E+DEGIPPNSIWKAARFGE
Sbjct: 800  FAAILDENQAIALARNPNVVSIFENQKRKLHTTRSWSFLGMESDEGIPPNSIWKAARFGE 859

Query: 140  DTIIANLDTGAWPESKSFNDAGYGPVPSRWMGVCEGGSNFTCNKKLIGARYFNKGFEAEN 199
            DTII NLDTGAWPESKSFNDAGYGPVPSRWMGVCEGG+NFTCNKKLIGARYFNKGFEAEN
Sbjct: 860  DTIIGNLDTGAWPESKSFNDAGYGPVPSRWMGVCEGGANFTCNKKLIGARYFNKGFEAEN 919

Query: 200  GPMSANLSTARDQEGHGSHTLSTAGGNFVPGANVFGNGNGTAKGGSPKARLAAYKVCWPS 259
            GPMSANL+TARDQEGHGSHTLSTAGGNFVPGANVFGNGNGTAKGGSP+ARLAAYKVCWPS
Sbjct: 920  GPMSANLTTARDQEGHGSHTLSTAGGNFVPGANVFGNGNGTAKGGSPRARLAAYKVCWPS 979

Query: 260  FNGGCYDADILAAIEAAIHDGVDVLSISLGSSARDFASDTLSVGAFHAVQQGIVVVCSGG 319
            F GGCYDADILAA+E+AIHDGVDVLSISLGSSARDFASDTLS+GAFHAVQQGIVVVCSGG
Sbjct: 980  FTGGCYDADILAAVESAIHDGVDVLSISLGSSARDFASDTLSIGAFHAVQQGIVVVCSGG 1039

Query: 320  NDGPTPGTVTNVSPWMFTVAASTIDRDFVNYVALGNKRHFEGVSLSSGGLPRGKFYPLVD 379
            NDGPTPGTVTNVSPWM TVAAST+DRDFVNYVALGNKRHF+GVSLSSGGLPRGKFYPLVD
Sbjct: 1040 NDGPTPGTVTNVSPWMITVAASTVDRDFVNYVALGNKRHFKGVSLSSGGLPRGKFYPLVD 1099

Query: 380  GVQVKAANATDNLALLCEDGSLDPVKAKGKIVLCLRGDSARMDKSFEVRRAGGVGLILVN 439
            GVQVKA NATD LALLCEDGSLDP KAKGKIVLCLRGDSARMDKSFEVRRAGG+GLILVN
Sbjct: 1100 GVQVKAGNATDKLALLCEDGSLDPAKAKGKIVLCLRGDSARMDKSFEVRRAGGIGLILVN 1159

Query: 440  DKQDGNDITADPHFLPASHLNYADGLAIFQYVNSTKSPMAFITHVKTELGIKPSPMVADF 499
            DK+DGNDITADPHFLPASHLNYADG+AIFQY+NSTKSPMAFITHVKTELGIKPSPMVADF
Sbjct: 1160 DKEDGNDITADPHFLPASHLNYADGIAIFQYINSTKSPMAFITHVKTELGIKPSPMVADF 1219

Query: 500  SSRGPNPIIDSMIKPDIAAPGVSILAAFSKYVTATDFPLDTRRVPFNFESGTSMACPHIS 559
            SSRGPNPIIDSMIKPDIAAPGVSILAAFS+Y TATDFPLDTRRV FNFESGTSMACPHIS
Sbjct: 1220 SSRGPNPIIDSMIKPDIAAPGVSILAAFSEYATATDFPLDTRRVSFNFESGTSMACPHIS 1279

Query: 560  GVVGLLKTLYPNWSPAAIKSAIMTTAKTRDNSMKSILDYNKVKATPFQYGAGHVHPNNAI 619
            GVVGLLKTLYP WSPAAIKSAIMTTAKTRDNSMKSILDYNK KATPFQYGAGHVHPNNAI
Sbjct: 1280 GVVGLLKTLYPKWSPAAIKSAIMTTAKTRDNSMKSILDYNKAKATPFQYGAGHVHPNNAI 1339

Query: 620  DPGLVYDTTIEDYMNFICAQGYNSTTLKKFYNKPFLCPKSFALADLNYPSISVPKLTAGV 679
            DPGLVYDTTIEDYMNFICAQGYNSTTLK+FYNKP+LCPKSF L DLNYPSISVPKLT GV
Sbjct: 1340 DPGLVYDTTIEDYMNFICAQGYNSTTLKRFYNKPYLCPKSFPLTDLNYPSISVPKLTIGV 1399

Query: 680  PATINRRLKNVGTPGTYVARVKVSSKVSVTVKPSTLQFNSVGEEKAFKVVFEYKGKGIGK 739
            P TINRRLKNVGTPGTYVARVKVSSKVSVTVKPSTLQFNSVGEEKAFKVVFEYKGKG GK
Sbjct: 1400 PVTINRRLKNVGTPGTYVARVKVSSKVSVTVKPSTLQFNSVGEEKAFKVVFEYKGKGQGK 1459

Query: 740  GHVFGTLIWSDGKHFVRSPMAVAL 764
            GHVFGTLIWSDGKHFVRSPM V L
Sbjct: 1460 GHVFGTLIWSDGKHFVRSPMVVKL 1481

BLAST of PI0002201 vs. NCBI nr
Match: XP_008449184.1 (PREDICTED: subtilisin-like protease SBT5.3 [Cucumis melo])

HSP 1 Score: 1424.1 bits (3685), Expect = 0.0e+00
Identity = 695/733 (94.82%), Postives = 714/733 (97.41%), Query Frame = 0

Query: 31   GSHFFGSNPSIYDVQLATESQYELLGSVVGSKLAAKESIFYSYNRYINGFAAILDENQAN 90
            GSHFFGSNPSI+DVQLATESQYELL SVVGSKLAAKESIFYSYNRYINGFAAILDENQA 
Sbjct: 778  GSHFFGSNPSIHDVQLATESQYELLESVVGSKLAAKESIFYSYNRYINGFAAILDENQAI 837

Query: 91   ALARNPNVVSIFENQKRKLHTTRSWSFLGVENDEGIPPNSIWKAARFGEDTIIANLDTGA 150
            ALARNPNVVSIFENQKRKLHTTRSWSFLG+E+DEGIPPNSIWKAARFGEDTII NLDTGA
Sbjct: 838  ALARNPNVVSIFENQKRKLHTTRSWSFLGMESDEGIPPNSIWKAARFGEDTIIGNLDTGA 897

Query: 151  WPESKSFNDAGYGPVPSRWMGVCEGGSNFTCNKKLIGARYFNKGFEAENGPMSANLSTAR 210
            WPESKSFNDAGYGPVPSRWMGVCEGG+NFTCNKKLIGARYFNKGFEAENGPMSANL+TAR
Sbjct: 898  WPESKSFNDAGYGPVPSRWMGVCEGGANFTCNKKLIGARYFNKGFEAENGPMSANLTTAR 957

Query: 211  DQEGHGSHTLSTAGGNFVPGANVFGNGNGTAKGGSPKARLAAYKVCWPSFNGGCYDADIL 270
            DQEGHGSHTLSTAGGNFVPGANVFGNGNGTAKGGSP+ARLAAYKVCWPSF GGCYDADIL
Sbjct: 958  DQEGHGSHTLSTAGGNFVPGANVFGNGNGTAKGGSPRARLAAYKVCWPSFTGGCYDADIL 1017

Query: 271  AAIEAAIHDGVDVLSISLGSSARDFASDTLSVGAFHAVQQGIVVVCSGGNDGPTPGTVTN 330
            AA+E+AIHDGVDVLSISLGSSARDFASDTLS+GAFHAVQQGIVVVCSGGNDGPTPGTVTN
Sbjct: 1018 AAVESAIHDGVDVLSISLGSSARDFASDTLSIGAFHAVQQGIVVVCSGGNDGPTPGTVTN 1077

Query: 331  VSPWMFTVAASTIDRDFVNYVALGNKRHFEGVSLSSGGLPRGKFYPLVDGVQVKAANATD 390
            VSPWM TVAAST+DRDFVNYVALGNKRHF+GVSLSSGGLPRGKFYPLVDGVQVKA NATD
Sbjct: 1078 VSPWMITVAASTVDRDFVNYVALGNKRHFKGVSLSSGGLPRGKFYPLVDGVQVKAGNATD 1137

Query: 391  NLALLCEDGSLDPVKAKGKIVLCLRGDSARMDKSFEVRRAGGVGLILVNDKQDGNDITAD 450
             LALLCEDGSLDP KAKGKIVLCLRGDSARMDKSFEVRRAGG+GLILVNDK+DGNDITAD
Sbjct: 1138 KLALLCEDGSLDPAKAKGKIVLCLRGDSARMDKSFEVRRAGGIGLILVNDKEDGNDITAD 1197

Query: 451  PHFLPASHLNYADGLAIFQYVNSTKSPMAFITHVKTELGIKPSPMVADFSSRGPNPIIDS 510
            PHFLPASHLNYADG+AIFQY+NSTKSPMAFITHVKTE+GIKPSPMVADFSSRGPNPIIDS
Sbjct: 1198 PHFLPASHLNYADGIAIFQYINSTKSPMAFITHVKTEMGIKPSPMVADFSSRGPNPIIDS 1257

Query: 511  MIKPDIAAPGVSILAAFSKYVTATDFPLDTRRVPFNFESGTSMACPHISGVVGLLKTLYP 570
            MIKPDIAAPGVSILAAFS+Y TATDFPLDTRRV FNFESGTSMACPHISGVVGLLKTLYP
Sbjct: 1258 MIKPDIAAPGVSILAAFSEYATATDFPLDTRRVSFNFESGTSMACPHISGVVGLLKTLYP 1317

Query: 571  NWSPAAIKSAIMTTAKTRDNSMKSILDYNKVKATPFQYGAGHVHPNNAIDPGLVYDTTIE 630
             WSPAAIKSAIMTTAKTRDNSMKSILDYNK KATPFQYGAGHVHPNNAIDPGLVYDTTIE
Sbjct: 1318 KWSPAAIKSAIMTTAKTRDNSMKSILDYNKAKATPFQYGAGHVHPNNAIDPGLVYDTTIE 1377

Query: 631  DYMNFICAQGYNSTTLKKFYNKPFLCPKSFALADLNYPSISVPKLTAGVPATINRRLKNV 690
            DYMNFICAQGYNSTTLK+FYNKP+LCPKSF L DLNYPSISVPKLT GVP TINRRLKNV
Sbjct: 1378 DYMNFICAQGYNSTTLKRFYNKPYLCPKSFPLTDLNYPSISVPKLTIGVPVTINRRLKNV 1437

Query: 691  GTPGTYVARVKVSSKVSVTVKPSTLQFNSVGEEKAFKVVFEYKGKGIGKGHVFGTLIWSD 750
            GTPGTYVARVKVSSKVSVTVKPSTLQFNSVGEEKAFKVVFEYKGKG GKGHVFGTLIWSD
Sbjct: 1438 GTPGTYVARVKVSSKVSVTVKPSTLQFNSVGEEKAFKVVFEYKGKGQGKGHVFGTLIWSD 1497

Query: 751  GKHFVRSPMAVAL 764
            G HFVRSPMAV L
Sbjct: 1498 GNHFVRSPMAVKL 1510

BLAST of PI0002201 vs. NCBI nr
Match: XP_038905648.1 (subtilisin-like protease SBT5.4 [Benincasa hispida])

HSP 1 Score: 1233.0 bits (3189), Expect = 0.0e+00
Identity = 588/734 (80.11%), Postives = 664/734 (90.46%), Query Frame = 0

Query: 31  GSHFFGSNPSIYDVQLATESQYELLGSVVGSKLAAKESIFYSYNRYINGFAAILDENQAN 90
           GSH FGSNP I+DVQLAT+SQY++LGSV+GSK+ AKES+ Y+YNR+INGFAA+LDEN+  
Sbjct: 29  GSHSFGSNP-IFDVQLATQSQYDILGSVMGSKMTAKESMIYTYNRHINGFAAVLDENEVM 88

Query: 91  ALARNPNVVSIFENQKRKLHTTRSWSFLGVENDEGIPPNSIWKAARFGEDTIIANLDTGA 150
           A+A+NPNVVS+FEN+KR+LHTTR+W FLG+END G+P NSIWKAARFGED II NLDTG 
Sbjct: 89  AIAQNPNVVSVFENKKRQLHTTRTWGFLGLENDAGVPSNSIWKAARFGEDIIIGNLDTGV 148

Query: 151 WPESKSFNDAGYGPVPSRWMGVCEGGSNFTCNKKLIGARYFNKGFEAENGPMSANLSTAR 210
           WPESKSF+DAGYGPVPSRWMGVCEGG NFTCNKKLIGARYF KGFEA NGP+  ++  AR
Sbjct: 149 WPESKSFSDAGYGPVPSRWMGVCEGGFNFTCNKKLIGARYFYKGFEAANGPIDDSVKFAR 208

Query: 211 DQEGHGSHTLSTAGGNFVPGANVFGNGNGTAKGGSPKARLAAYKVCWPSFNGGCYDADIL 270
           D EGHGSHTLSTAGGNFVPGANVFGNGNGTAKGGSPKAR+AAYKVCWP+  GGC+DADIL
Sbjct: 209 DLEGHGSHTLSTAGGNFVPGANVFGNGNGTAKGGSPKARVAAYKVCWPALTGGCFDADIL 268

Query: 271 AAIEAAIHDGVDVLSISLGSSARDFASDTLSVGAFHAVQQGIVVVCSGGNDGPTPGTVTN 330
           AA EAAI DGVDVLS+SLGS A+DFASD +++GAFHAVQ+GI VVCS GNDGP+P TVTN
Sbjct: 269 AAFEAAISDGVDVLSVSLGSPAQDFASDPVAIGAFHAVQEGITVVCSAGNDGPSPSTVTN 328

Query: 331 VSPWMFTVAASTIDRDFVNYVALGNKRHFEGVSLSSGGLPRGKFYPLVDGVQVKAANATD 390
           V+PWMFTVAAST+DRDF +YV LGNK+ F+G+SLS+GG P G FYPL+DGVQVKAAN TD
Sbjct: 329 VAPWMFTVAASTVDRDFFSYVTLGNKKQFKGLSLSAGGTPGGNFYPLMDGVQVKAANITD 388

Query: 391 NLALLCEDGSLDPVKAKGKIVLCLRGDSARMDKSFEVRRAGGVGLILVNDKQDGNDITAD 450
           NLA LCE+GSLDP KAKGKI+LCLRGD+AR+DK  EV R GGVG+ILVNDK DGND++AD
Sbjct: 389 NLAQLCEEGSLDPTKAKGKIILCLRGDNARLDKGVEVLRVGGVGMILVNDKLDGNDVSAD 448

Query: 451 PHFLPASHLNYADGLAIFQYVNSTKSPMAFITHVKTELGIKPSPMVADFSSRGPNPIIDS 510
            HFLPASHLNY DGL IFQY+NST+SP+A I+HV+TELGIKPSPM+ DFSSRGPNPIIDS
Sbjct: 449 RHFLPASHLNYTDGLEIFQYLNSTQSPVASISHVETELGIKPSPMMGDFSSRGPNPIIDS 508

Query: 511 MIKPDIAAPGVSILAAFSKYVTATDFPLDTRRVPFNFESGTSMACPHISGVVGLLKTLYP 570
           MIKPDI APG++I+AA S+  TAT    DTRRVPFNFE GTSM+CPHISGVVGLLKTLYP
Sbjct: 509 MIKPDITAPGLNIIAAVSESATATSSAYDTRRVPFNFEIGTSMSCPHISGVVGLLKTLYP 568

Query: 571 NWSPAAIKSAIMTTAKTRDNSMKSILDYNKVKATPFQYGAGHVHPNNAIDPGLVYDTTIE 630
            WSPAAI+SAIMTTAKTRDNS KSILDYNK+KATPF YGAGHVHPNNA+DPGLVYDTT E
Sbjct: 569 KWSPAAIRSAIMTTAKTRDNSKKSILDYNKMKATPFHYGAGHVHPNNAMDPGLVYDTTTE 628

Query: 631 DYMNFICAQGYNSTTLKKFYNKPFLCPKSFALADLNYPSISVPKLTAGVPATINRRLKNV 690
           DYMNF+CA GYN TTL++FYNKP++CP+SFA+ DLNYPSISVPKLTAGVP TINR++KNV
Sbjct: 629 DYMNFLCALGYNYTTLREFYNKPYVCPESFAVMDLNYPSISVPKLTAGVPVTINRKVKNV 688

Query: 691 GTPGTYVARVKVSSKVSVTVKPSTLQFNSVGEEKAFKVVFEYKGKGIGKGHVFGTLIWSD 750
           G+PGTYVARVKVSS VSVTV+PSTLQF+S GEEKAFKVVF+YKG G GK HVFGTLIWSD
Sbjct: 689 GSPGTYVARVKVSSMVSVTVEPSTLQFDSEGEEKAFKVVFQYKGTGQGKDHVFGTLIWSD 748

Query: 751 GKHFVRSPMAVALA 765
           GKHFVRSPMA+ALA
Sbjct: 749 GKHFVRSPMAMALA 761

BLAST of PI0002201 vs. NCBI nr
Match: XP_038905686.1 (subtilisin-like protease SBT5.3 [Benincasa hispida])

HSP 1 Score: 1167.1 bits (3018), Expect = 0.0e+00
Identity = 551/733 (75.17%), Postives = 645/733 (87.99%), Query Frame = 0

Query: 31  GSHFFGSNPSIYDVQLATESQYELLGSVVGSKLAAKESIFYSYNRYINGFAAILDENQAN 90
           G H FG NPS YDVQ ATESQY++L SV GSKLAAKESI YSY+RYINGFAA+LDE +A 
Sbjct: 33  GLHSFGLNPSKYDVQRATESQYDILSSVTGSKLAAKESIMYSYSRYINGFAAVLDEKEAM 92

Query: 91  ALARNPNVVSIFENQKRKLHTTRSWSFLGVENDEGIPPNSIWKAARFGEDTIIANLDTGA 150
           ALA+NP+VVS+FEN++RKLHTT+SWSFLGV++D GIP NSIWKAARFGEDTII NLDTGA
Sbjct: 93  ALAKNPSVVSVFENKERKLHTTKSWSFLGVDSDAGIPSNSIWKAARFGEDTIIGNLDTGA 152

Query: 151 WPESKSFNDAGYGPVPSRWMGVCEGGSNFTCNKKLIGARYFNKGFEAENGPMSANLSTAR 210
           WPESKSFNDAGYGPVPSRW G C+GG+NF CN+KLIGARYFN+GF   NGP++ + +TAR
Sbjct: 153 WPESKSFNDAGYGPVPSRWRGACDGGANFRCNRKLIGARYFNQGFAMANGPLNVSFNTAR 212

Query: 211 DQEGHGSHTLSTAGGNFVPGANVFGNGNGTAKGGSPKARLAAYKVCWPSFNGGCYDADIL 270
           D+EGHGSHTLSTAGGNFVPGAN+FG GNGTAKGGSPKAR+AAYKVCWP+  GGC+D+DIL
Sbjct: 213 DKEGHGSHTLSTAGGNFVPGANIFGYGNGTAKGGSPKARVAAYKVCWPAPTGGCFDSDIL 272

Query: 271 AAIEAAIHDGVDVLSISLGSSARDFASDTLSVGAFHAVQQGIVVVCSGGNDGPTPGTVTN 330
           A  EAAI DGVDVLS+SLG+ A++FA D +S+GAFHAVQ+GIVVVCSGGNDGP+PGTV+N
Sbjct: 273 AGFEAAIGDGVDVLSVSLGTGAQEFAYDAVSIGAFHAVQKGIVVVCSGGNDGPSPGTVSN 332

Query: 331 VSPWMFTVAASTIDRDFVNYVALGNKRHFEGVSLSSGGLPRGKFYPLVDGVQVKAANATD 390
           VSPWMFTVAASTIDRDF +YV LGNK+H  G SLSS GL   KFYPL++ ++ KAANATD
Sbjct: 333 VSPWMFTVAASTIDRDFASYVLLGNKKHIRGSSLSSSGLRGRKFYPLINAIEAKAANATD 392

Query: 391 NLALLCEDGSLDPVKAKGKIVLCLRGDSARMDKSFEVRRAGGVGLILVNDKQDGNDITAD 450
           +LA  CE GSLDP KAKGKI++CLRG++AR++KSF V  AGGVG+I+VND++DG+   AD
Sbjct: 393 SLAQFCEQGSLDPAKAKGKIIVCLRGENARVEKSFVVLHAGGVGMIMVNDQKDGSGTLAD 452

Query: 451 PHFLPASHLNYADGLAIFQYVNSTKSPMAFITHVKTELGIKPSPMVADFSSRGPNPIIDS 510
            H LPA+H++Y DGL+I QY+ STK+P+A+ITHVKTE+GIKPSP++ADFSSRGPN I ++
Sbjct: 453 AHILPATHVSYTDGLSISQYIKSTKTPVAYITHVKTEVGIKPSPVMADFSSRGPNSITEA 512

Query: 511 MIKPDIAAPGVSILAAFSKYVTATDFPLDTRRVPFNFESGTSMACPHISGVVGLLKTLYP 570
           M+KPDI APGV+I+A+ +   TATD P DTRRVPFN ESGTSM+CPHISGV GLLKTLYP
Sbjct: 513 MLKPDITAPGVNIIASVTIDATATDSPFDTRRVPFNVESGTSMSCPHISGVAGLLKTLYP 572

Query: 571 NWSPAAIKSAIMTTAKTRDNSMKSILDYNKVKATPFQYGAGHVHPNNAIDPGLVYDTTIE 630
            WSPAAIKSAIMTTAKTRDN+ ++I D  K+KATPF YGAGHVHPN+A+DPGLVYDTTI+
Sbjct: 573 TWSPAAIKSAIMTTAKTRDNTQRTISDTAKMKATPFDYGAGHVHPNSAMDPGLVYDTTID 632

Query: 631 DYMNFICAQGYNSTTLKKFYNKPFLCPKSFALADLNYPSISVPKLTAGVPATINRRLKNV 690
           DY+NF+CA+GYNS  LKKFYNKPF+C KSFA+ DLNYPSISVP+L  G P T+NRR+KNV
Sbjct: 633 DYLNFLCARGYNSVALKKFYNKPFICAKSFAITDLNYPSISVPELRIGAPVTVNRRVKNV 692

Query: 691 GTPGTYVARVKVSSKVSVTVKPSTLQFNSVGEEKAFKVVFEYKGKGIGKGHVFGTLIWSD 750
           GTPGTYVARVK S  +SV+V+PSTLQFNSVGEEKAFKVVF+YKGK   +GHVFGTLIWSD
Sbjct: 693 GTPGTYVARVKASPAISVSVEPSTLQFNSVGEEKAFKVVFQYKGKEQRQGHVFGTLIWSD 752

Query: 751 GKHFVRSPMAVAL 764
           GKHFVRSP+AV L
Sbjct: 753 GKHFVRSPIAVKL 765

BLAST of PI0002201 vs. NCBI nr
Match: XP_031738719.1 (uncharacterized protein LOC101212014 [Cucumis sativus])

HSP 1 Score: 1144.8 bits (2960), Expect = 0.0e+00
Identity = 547/734 (74.52%), Postives = 634/734 (86.38%), Query Frame = 0

Query: 31  GSHFFGSNPSIYDVQLATESQYELLGSVVGSKLAAKESIFYSYNRYINGFAAILDENQAN 90
           G+   GSNP+ YD+++ATESQY+LLGSVVGSKLAAK++I YSYN+YINGFAA LDE QA 
Sbjct: 61  GAPSVGSNPTNYDIEVATESQYDLLGSVVGSKLAAKDAIKYSYNKYINGFAATLDEKQAK 120

Query: 91  ALARNPNVVSIFENQKRKLHTTRSWSFLGVENDEGIPPNSIWKAARFGEDTIIANLDTGA 150
            LA+NP VVS+FEN++RKLHTTRSW FLGVE+DEGIP NSIW A RFGEDTII NLDTG 
Sbjct: 121 DLAKNPKVVSVFENKERKLHTTRSWHFLGVESDEGIPSNSIWNAGRFGEDTIIGNLDTGV 180

Query: 151 WPESKSFNDAGYGPVPSRWMGVCEGGSNFTCNKKLIGARYFNKGFEAENGPMSANLSTAR 210
           WPESKSFNDAGYGPVPSRW G CEGG+NF CN+KLIGARYFNKGF   +GP++ + +TAR
Sbjct: 181 WPESKSFNDAGYGPVPSRWRGACEGGANFRCNRKLIGARYFNKGFAMASGPLNISFNTAR 240

Query: 211 DQEGHGSHTLSTAGGNFVPGANVFGNGNGTAKGGSPKARLAAYKVCWPSFN-GGCYDADI 270
           D++GHGSHTLSTAGGNFVPGANVFG GNGTAKGGSPKAR+AAYKVCWP+ + GGCYDADI
Sbjct: 241 DKQGHGSHTLSTAGGNFVPGANVFGYGNGTAKGGSPKARVAAYKVCWPATSGGGCYDADI 300

Query: 271 LAAIEAAIHDGVDVLSISLGSSARDFASDTLSVGAFHAVQQGIVVVCSGGNDGPTPGTVT 330
           LA  EAAI DGVDVLS+SLGS   +FA D++S+GAFHAVQQGIVVVCS GNDGP PGTV+
Sbjct: 301 LAGFEAAISDGVDVLSVSLGSKPEEFAYDSMSIGAFHAVQQGIVVVCSAGNDGPGPGTVS 360

Query: 331 NVSPWMFTVAASTIDRDFVNYVALGNKRHFEGVSLSSGGLPRGKFYPLVDGVQVKAANAT 390
           N+SPWMFTVAAS+IDRDF +Y +LGNK+H++G S+SS  L  GKFYPL++ V  KAANA+
Sbjct: 361 NISPWMFTVAASSIDRDFTSYASLGNKKHYKGSSISSSALAGGKFYPLINAVDAKAANAS 420

Query: 391 DNLALLCEDGSLDPVKAKGKIVLCLRGDSARMDKSFEVRRAGGVGLILVNDKQDGNDITA 450
           + LA LC  GSLDP KAKGKI++CLRG++AR++K F V +AGGVG+ILVN K  G+  TA
Sbjct: 421 EILAQLCHKGSLDPTKAKGKIIVCLRGENARVEKGFVVLQAGGVGMILVNGKNGGSGTTA 480

Query: 451 DPHFLPASHLNYADGLAIFQYVNSTKSPMAFITHVKTELGIKPSPMVADFSSRGPNPIID 510
           D H LPA+HL+Y DGLA+ QY+NSTK+P+A IT V+T+LGIKPSP++ADFSSRGPNPI +
Sbjct: 481 DAHILPATHLSYTDGLAVAQYINSTKTPVAHITPVQTQLGIKPSPVMADFSSRGPNPITE 540

Query: 511 SMIKPDIAAPGVSILAAFSKYVTATDFPLDTRRVPFNFESGTSMACPHISGVVGLLKTLY 570
           +M+KPDI  PG+SILA+ +  VTAT FP DTRRVPFN ESGTSM+CPHISGVVGLLKTLY
Sbjct: 541 AMLKPDITGPGMSILASVTTDVTATTFPFDTRRVPFNVESGTSMSCPHISGVVGLLKTLY 600

Query: 571 PNWSPAAIKSAIMTTAKTRDNSMKSILDYNKVKATPFQYGAGHVHPNNAIDPGLVYDTTI 630
           P WSPAAIKSAIMTTAKTRDN+M++I D  K KATPF YGAGHVHPN+A+DPGLVYDTTI
Sbjct: 601 PTWSPAAIKSAIMTTAKTRDNTMRTISDNVKPKATPFDYGAGHVHPNSAMDPGLVYDTTI 660

Query: 631 EDYMNFICAQGYNSTTLKKFYNKPFLCPKSFALADLNYPSISVPKLTAGVPATINRRLKN 690
           +DY+NF+CA+GYNS T K FYNKPF+C KSF L DLNYPSIS+PKL  G P T+NRR+KN
Sbjct: 661 DDYLNFLCARGYNSLTFKNFYNKPFVCAKSFTLTDLNYPSISIPKLQFGAPVTVNRRVKN 720

Query: 691 VGTPGTYVARVKVSSKVSVTVKPSTLQFNSVGEEKAFKVVFEYKGKGIGKGHVFGTLIWS 750
           VGTPGTYVARV  SSK+ VTV+PSTLQFNSVGEEKAFKVVFEYKG    KG+VFGTLIWS
Sbjct: 721 VGTPGTYVARVNASSKILVTVEPSTLQFNSVGEEKAFKVVFEYKGNEQDKGYVFGTLIWS 780

Query: 751 DGKHFVRSPMAVAL 764
           DGKH VRSP+ V L
Sbjct: 781 DGKHNVRSPIVVNL 794

BLAST of PI0002201 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 820.1 bits (2117), Expect = 1.5e-237
Identity = 395/738 (53.52%), Postives = 527/738 (71.41%), Query Frame = 0

Query: 30  FGSHFFGSNPSIYDVQLATESQYELLGSVVGSKLAAKESIFYSYNRYINGFAAILDENQA 89
           FG+H      +   +    E+ Y+ LGS  GS+  A ++IFYSY ++INGFAA LD + A
Sbjct: 36  FGAHSHVGEITEDAMDRVKETHYDFLGSFTGSRERATDAIFYSYTKHINGFAAHLDHDLA 95

Query: 90  NALARNPNVVSIFENQKRKLHTTRSWSFLGVENDEGIPPNSIWKAARFGEDTIIANLDTG 149
             ++++P VVS+F N+  KLHTTRSW FLG+E++  +P +SIW+ ARFGEDTIIANLDTG
Sbjct: 96  YEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHNSYVPSSSIWRKARFGEDTIIANLDTG 155

Query: 150 AWPESKSFNDAGYGPVPSRWMGVCEG--GSNFTCNKKLIGARYFNKGFEAENGPMSANLS 209
            WPESKSF D G GP+PSRW G+C+    + F CN+KLIGARYFNKG+ A  G ++++  
Sbjct: 156 VWPESKSFRDEGLGPIPSRWKGICQNQKDATFHCNRKLIGARYFNKGYAAAVGHLNSSFD 215

Query: 210 TARDQEGHGSHTLSTAGGNFVPGANVFGNGNGTAKGGSPKARLAAYKVCWPSFNGG-CYD 269
           + RD +GHGSHTLSTA G+FVPG ++FG GNGTAKGGSP+AR+AAYKVCWP   G  CYD
Sbjct: 216 SPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTAKGGSPRARVAAYKVCWPPVKGNECYD 275

Query: 270 ADILAAIEAAIHDGVDVLSISLGSSARDFASDTLSVGAFHAVQQGIVVVCSGGNDGPTPG 329
           AD+LAA +AAIHDG DV+S+SLG     F +D++++G+FHA ++ IVVVCS GN GP   
Sbjct: 276 ADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADS 335

Query: 330 TVTNVSPWMFTVAASTIDRDFVNYVALGNKRHFEGVSLSSGGLPRGKFYPLVDGVQVKAA 389
           TV+NV+PW  TV AST+DR+F + + LGN +H++G SLSS  LP  KFYP++  V  KA 
Sbjct: 336 TVSNVAPWQITVGASTMDREFASNLVLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKAK 395

Query: 390 NATDNLALLCEDGSLDPVKAKGKIVLCLRGDSARMDKSFEVRRAGGVGLILVNDKQDGND 449
           NA+   A LC+ GSLDP+K KGKI++CLRG + R++K   V   GG+G++L N    GND
Sbjct: 396 NASALDAQLCKLGSLDPIKTKGKILVCLRGQNGRVEKGRAVALGGGIGMVLENTYVTGND 455

Query: 450 ITADPHFLPASHLNYADGLAIFQYVNSTKSPMAFITHVKTELGIKPSPMVADFSSRGPNP 509
           + ADPH LPA+ L   D  A+ +Y++ TK P+A IT  +T+LG+KP+P++A FSS+GP+ 
Sbjct: 456 LLADPHVLPATQLTSKDSFAVSRYISQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSI 515

Query: 510 IIDSMIKPDIAAPGVSILAAFSKYVTATDFPLDTRRVPFNFESGTSMACPHISGVVGLLK 569
           +   ++KPDI APGVS++AA++  V+ T+   D RR+ FN  SGTSM+CPHISG+ GLLK
Sbjct: 516 VAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPRRLLFNAISGTSMSCPHISGIAGLLK 575

Query: 570 TLYPNWSPAAIKSAIMTTAKTRDNSMKSILDYNKVKATPFQYGAGHVHPNNAIDPGLVYD 629
           T YP+WSPAAI+SAIMTTA   D+    I +   +KATPF +GAGHV PN A++PGLVYD
Sbjct: 576 TRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATNMKATPFSFGAGHVQPNLAVNPGLVYD 635

Query: 630 TTIEDYMNFICAQGYNSTTLKKFYNKPFLCPK-SFALADLNYPSISVPKLTAGVPATINR 689
             I+DY+NF+C+ GYN++ +  F    F C     +L +LNYPSI+VP LT+    T++R
Sbjct: 636 LGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPKISLVNLNYPSITVPNLTSS-KVTVSR 695

Query: 690 RLKNVGTPGTYVARVKVSSKVSVTVKPSTLQFNSVGEEKAFKVVFEYKGKGIGKGHVFGT 749
            +KNVG P  Y  +V     V V VKP++L F  VGE+K FKV+       + KG+VFG 
Sbjct: 696 TVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKVGEQKTFKVILVKSKGNVAKGYVFGE 755

Query: 750 LIWSDGKHFVRSPMAVAL 764
           L+WSD KH VRSP+ V L
Sbjct: 756 LVWSDKKHRVRSPIVVKL 772

BLAST of PI0002201 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 808.5 bits (2087), Expect = 4.5e-234
Identity = 409/737 (55.50%), Postives = 513/737 (69.61%), Query Frame = 0

Query: 31  GSHFFGSNPSIYDVQLATESQYELLGSVVGSKLAAKESIFYSYNRYINGFAAILDENQAN 90
           GSH      S   +     S    L S VGS   AKE+IFYSY R+INGFAAILDEN+A 
Sbjct: 47  GSHAHLPQISSAHLDGVAHSHRTFLASFVGSHENAKEAIFYSYKRHINGFAAILDENEAA 106

Query: 91  ALARNPNVVSIFENQKRKLHTTRSWSFLGVENDEGIPPNSIWKAARFGEDTIIANLDTGA 150
            +A++P+VVS+F N+ RKLHTT SW+F+ +  +  +  +S+W  A +GEDTIIANLDTG 
Sbjct: 107 EIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKNGVVHKSSLWNKAGYGEDTIIANLDTGV 166

Query: 151 WPESKSFNDAGYGPVPSRWMGVCEGGSNFTCNKKLIGARYFNKGFEAENG-PMSANLSTA 210
           WPESKSF+D GYG VP+RW G C    +  CN+KLIGARYFNKG+ A  G P +A+  T 
Sbjct: 167 WPESKSFSDEGYGAVPARWKGRCH--KDVPCNRKLIGARYFNKGYLAYTGLPSNASYETC 226

Query: 211 RDQEGHGSHTLSTAGGNFVPGANVFGNGNGTAKGGSPKARLAAYKVCWPSFNGG-CYDAD 270
           RD +GHGSHTLSTA GNFVPGANVFG GNGTA GGSPKAR+AAYKVCWP  +G  C+DAD
Sbjct: 227 RDHDGHGSHTLSTAAGNFVPGANVFGIGNGTASGGSPKARVAAYKVCWPPVDGAECFDAD 286

Query: 271 ILAAIEAAIHDGVDVLSISLGSSARDFASDTLSVGAFHAVQQGIVVVCSGGNDGPTPGTV 330
           ILAAIEAAI DGVDVLS S+G  A D+ SD +++G+FHAV+ G+ VVCS GN GP  GTV
Sbjct: 287 ILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTV 346

Query: 331 TNVSPWMFTVAASTIDRDFVNYVALGNKRHFEGVSLSSGGLPRGKFYPLVDGVQVKAANA 390
           +NV+PW+ TV AS++DR+F  +V L N + F+G SLS   LP  K Y L+       AN 
Sbjct: 347 SNVAPWVITVGASSMDREFQAFVELKNGQSFKGTSLSK-PLPEEKMYSLISAADANVANG 406

Query: 391 TDNLALLCEDGSLDPVKAKGKIVLCLRGDSARMDKSFEVRRAGGVGLILVNDKQDGNDIT 450
               ALLC+ GSLDP K KGKI++CLRGD+AR+DK  +   AG  G++L NDK  GN+I 
Sbjct: 407 NVTDALLCKKGSLDPKKVKGKILVCLRGDNARVDKGMQAAAAGAAGMVLCNDKASGNEII 466

Query: 451 ADPHFLPASHLNYADGLAIFQYVNSTKSPMAFITHVKTELGIKPSPMVADFSSRGPNPII 510
           +D H LPAS ++Y DG  +F Y++STK P  +I      L  KP+P +A FSSRGPN I 
Sbjct: 467 SDAHVLPASQIDYKDGETLFSYLSSTKDPKGYIKAPTATLNTKPAPFMASFSSRGPNTIT 526

Query: 511 DSMIKPDIAAPGVSILAAFSKYVTATDFPLDTRRVPFNFESGTSMACPHISGVVGLLKTL 570
             ++KPDI APGV+I+AAF++    TD   D RR PFN ESGTSM+CPHISGVVGLLKTL
Sbjct: 527 PGILKPDITAPGVNIIAAFTEATGPTDLDSDNRRTPFNTESGTSMSCPHISGVVGLLKTL 586

Query: 571 YPNWSPAAIKSAIMTTAKTRDNSMKSILDYNKVKATPFQYGAGHVHPNNAIDPGLVYDTT 630
           +P+WSPAAI+SAIMTT++TR+N  K ++D +  KA PF YG+GHV PN A  PGLVYD T
Sbjct: 587 HPHWSPAAIRSAIMTTSRTRNNRRKPMVDESFKKANPFSYGSGHVQPNKAAHPGLVYDLT 646

Query: 631 IEDYMNFICAQGYNSTTLKKFYNKP-FLCPKSFALADLNYPSISVPKLTAGVPATINRRL 690
             DY++F+CA GYN+T ++ F   P + C +   L D NYPSI+VP LT  +  T+ R+L
Sbjct: 647 TGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGANLLDFNYPSITVPNLTGSI--TVTRKL 706

Query: 691 KNVGTPGTYVARVKVSSKVSVTVKPSTLQFNSVGEEKAFKVVFEYKGKGIGKGHVFGTLI 750
           KNVG P TY AR +    V V+V+P  L FN  GE K F++           G+VFG L 
Sbjct: 707 KNVGPPATYNARFREPLGVRVSVEPKQLTFNKTGEVKIFQMTLR-PLPVTPSGYVFGELT 766

Query: 751 WSDGKHFVRSPMAVALA 765
           W+D  H+VRSP+ V L+
Sbjct: 767 WTDSHHYVRSPIVVQLS 777

BLAST of PI0002201 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 597.4 bits (1539), Expect = 1.6e-170
Identity = 333/758 (43.93%), Postives = 472/758 (62.27%), Query Frame = 0

Query: 25  ILHCLFGSHF--FGSNPSIYDVQLATE---SQYELLGSVVGSKLAA---KESIFYSYNRY 84
           +L CL   H     S+   Y V +A     S ++L  +   S L +      + Y+Y   
Sbjct: 13  LLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAELLYTYENA 72

Query: 85  INGFAAILDENQANALARNPNVVSIFENQKRKLHTTRSWSFLGVENDEGIPPNSIWKAAR 144
           I+GF+  L + +A++L   P V+S+    + +LHTTR+  FLG++         ++  A 
Sbjct: 73  IHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTA----DLFPEAG 132

Query: 145 FGEDTIIANLDTGAWPESKSFNDAGYGPVPSRWMGVCEGGSNFT---CNKKLIGARYFNK 204
              D ++  LDTG WPESKS++D G+GP+PS W G CE G+NFT   CN+KLIGAR+F +
Sbjct: 133 SYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFAR 192

Query: 205 GFEAENGPM--SANLSTARDQEGHGSHTLSTAGGNFVPGANVFGNGNGTAKGGSPKARLA 264
           G+E+  GP+  S    + RD +GHG+HT STA G+ V GA++ G  +GTA+G +P+AR+A
Sbjct: 193 GYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVA 252

Query: 265 AYKVCWPSFNGGCYDADILAAIEAAIHDGVDVLSISLGSSARDFASDTLSVGAFHAVQQG 324
            YKVCW    GGC+ +DILAAI+ AI D V+VLS+SLG    D+  D +++GAF A+++G
Sbjct: 253 VYKVCW---LGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERG 312

Query: 325 IVVVCSGGNDGPTPGTVTNVSPWMFTVAASTIDRDFVNYVALGNKRHFEGVSLSSGGLPR 384
           I+V CS GN GP+  +++NV+PW+ TV A T+DRDF     LGN ++F GVSL  G    
Sbjct: 313 ILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALP 372

Query: 385 GKFYPLVDGVQVKAANATDNLALLCEDGSLDPVKAKGKIVLCLRGDSARMDKSFEVRRAG 444
            K  P +      A+NAT+    LC  G+L P K KGKIV+C RG +AR+ K   V+ AG
Sbjct: 373 DKLLPFI--YAGNASNATN--GNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAG 432

Query: 445 GVGLILVNDKQDGNDITADPHFLPASHLNYADGLAIFQYVNSTKSPMAFITHVKTELGIK 504
           GVG+IL N   +G ++ AD H LPA+ +    G  I  YV +  +P A I+ + T +G+K
Sbjct: 433 GVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVK 492

Query: 505 PSPMVADFSSRGPNPIIDSMIKPDIAAPGVSILAAFSKYVTATDFPLDTRRVPFNFESGT 564
           PSP+VA FSSRGPN I  +++KPD+ APGV+ILAA++     T    D+RRV FN  SGT
Sbjct: 493 PSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGT 552

Query: 565 SMACPHISGVVGLLKTLYPNWSPAAIKSAIMTTAKTRDNSMKSILDYNKVK-ATPFQYGA 624
           SM+CPH+SG+  LLK+++P WSPAAI+SA+MTTA       K +LD    K +TPF +GA
Sbjct: 553 SMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGA 612

Query: 625 GHVHPNNAIDPGLVYDTTIEDYMNFICAQGYNSTTLKKFYNKPFLC--PKSFALADLNYP 684
           GHV P  A +PGL+YD T EDY+ F+CA  Y S  ++    + + C   KS+++ADLNYP
Sbjct: 613 GHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYP 672

Query: 685 SISVPKLTAGVPA-TINRRLKNVGTPGTYVARVKVSSK---VSVTVKPSTLQFNSVGEEK 744
           S +V     GV A    R + +VG  GTY   VKV+S+   V ++V+P+ L F    E+K
Sbjct: 673 SFAVN--VDGVGAYKYTRTVTSVGGAGTY--SVKVTSETTGVKISVEPAVLNFKEANEKK 732

Query: 745 AFKVVFEYKGKGIGKGHVFGTLIWSDGKHFVRSPMAVA 763
           ++ V F          + FG++ WSDGKH V SP+A++
Sbjct: 733 SYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

BLAST of PI0002201 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 564.3 bits (1453), Expect = 1.5e-160
Identity = 312/717 (43.51%), Postives = 443/717 (61.79%), Query Frame = 0

Query: 68  SIFYSYNRYINGFAAILDENQANALARNPNVVSIFENQKRKLHTTRSWSFLGVENDEGIP 127
           ++ YSY+R ++GF+A L   Q  AL R+P+V+S+  +Q R++HTT + +FLG   + G  
Sbjct: 68  TLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSG-- 127

Query: 128 PNSIWKAARFGEDTIIANLDTGAWPESKSFNDAGYGPVPSRWMGVCEGGSNF---TCNKK 187
              +W  + +GED I+  LDTG WPE  SF+D+G GP+PS W G CE G +F   +CN+K
Sbjct: 128 ---LWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRK 187

Query: 188 LIGARYFNKGFEAENGPMSANLS----TARDQEGHGSHTLSTAGGNFVPGANVFGNGNGT 247
           LIGAR F +G+  +      + +    + RD EGHG+HT STA G+ V  A+++    GT
Sbjct: 188 LIGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGT 247

Query: 248 AKGGSPKARLAAYKVCWPSFNGGCYDADILAAIEAAIHDGVDVLSISLGS--SARDFASD 307
           A G + KAR+AAYK+CW    GGCYD+DILAA++ A+ DGV V+S+S+G+  SA ++ +D
Sbjct: 248 ATGMASKARIAAYKICW---TGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTD 307

Query: 308 TLSVGAFHAVQQGIVVVCSGGNDGPTPGTVTNVSPWMFTVAASTIDRDFVNYVALGNKRH 367
           ++++GAF A + GIVV CS GN GP P T TN++PW+ TV AST+DR+F      G+ + 
Sbjct: 308 SIAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKV 367

Query: 368 FEGVSLSSG-GLPRGKFYPLVDGVQVKAANATDNLALLCEDGSLDPVKAKGKIVLCLRGD 427
           F G SL +G  LP           Q+    + D  + LC  G L+    +GKIVLC RG 
Sbjct: 368 FTGTSLYAGESLPDS---------QLSLVYSGDCGSRLCYPGKLNSSLVEGKIVLCDRGG 427

Query: 428 SARMDKSFEVRRAGGVGLILVNDKQDGNDITADPHFLPASHLNYADGLAIFQYVNSTKSP 487
           +AR++K   V+ AGG G+IL N  + G ++TAD H +PA+ +    G  I  Y+ ++ SP
Sbjct: 428 NARVEKGSAVKLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSP 487

Query: 488 MAFITHVKTELG-IKPSPMVADFSSRGPNPIIDSMIKPDIAAPGVSILAAFSKYVTATDF 547
            A I+ + T +G   PSP VA FSSRGPN +   ++KPD+ APGV+ILA ++  V  TD 
Sbjct: 488 TAKISFLGTLIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDL 547

Query: 548 PLDTRRVPFNFESGTSMACPHISGVVGLLKTLYPNWSPAAIKSAIMTTAKTRDNSMKSIL 607
            +D RRV FN  SGTSM+CPH+SG+  LL+  +P+WSPAAIKSA++TTA   +NS + I 
Sbjct: 548 DIDPRRVQFNIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIE 607

Query: 608 DYNKVKAT-PFQYGAGHVHPNNAIDPGLVYDTTIEDYMNFICAQGYNSTTLKKFYNKPFL 667
           D    K++  F +GAGHV PN A++PGLVYD  +++Y+ F+CA GY    +  F   P L
Sbjct: 608 DLATGKSSNSFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTL 667

Query: 668 -----CPKSFALADLNYPSISVPKLTAGVPATINRRLKNVGT--PGTYVARVKVSSKVSV 727
                  K     DLNYPS SV   + G      R +KNVG+     Y   VK  + V +
Sbjct: 668 YDACETSKLRTAGDLNYPSFSVVFASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEI 727

Query: 728 TVKPSTLQFNSVGEEKAFKVVFE--YKGKGIGK--GHVFGTLIWSDGKHFVRSPMAV 762
            V PS L F+       ++V F+    G G+G   GH FG++ W+DG+H V+SP+AV
Sbjct: 728 DVSPSKLAFSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAV 767

BLAST of PI0002201 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 563.9 bits (1452), Expect = 1.9e-160
Identity = 313/746 (41.96%), Postives = 454/746 (60.86%), Query Frame = 0

Query: 36  GSNPSIYDVQLATESQYELLGSVVGSKLAAKESIFYSYNRYINGFAAILDENQANALARN 95
           GS PSI+       S          ++ A +  I + Y+   +GF+A++  ++A+ L  +
Sbjct: 35  GSMPSIFPTHYHWYS----------TEFAEESRIVHVYHTVFHGFSAVVTPDEADNLRNH 94

Query: 96  PNVVSIFENQKRKLHTTRSWSFLGVENDEGIPPNSIWKAARFGEDTIIANLDTGAWPESK 155
           P V+++FE+++R+LHTTRS  FLG++N +G     +W  + +G D II   DTG WPE +
Sbjct: 95  PAVLAVFEDRRRELHTTRSPQFLGLQNQKG-----LWSESDYGSDVIIGVFDTGIWPERR 154

Query: 156 SFNDAGYGPVPSRWMGVCEGGSNFT---CNKKLIGARYFNKGFEAE---NGPMSANLSTA 215
           SF+D   GP+P RW GVCE G+ F+   CN+K+IGAR+F KG +A        +    + 
Sbjct: 155 SFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGARFFAKGQQAAVIGGINKTVEFLSP 214

Query: 216 RDQEGHGSHTLSTAGGNFVPGANVFGNGNGTAKGGSPKARLAAYKVCWPSFNGGCYDADI 275
           RD +GHG+HT STA G     A++ G  +G AKG +PKAR+AAYKVCW   + GC D+DI
Sbjct: 215 RDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPKARIAAYKVCWK--DSGCLDSDI 274

Query: 276 LAAIEAAIHDGVDVLSISLGSS---ARDFASDTLSVGAFHAVQQGIVVVCSGGNDGPTPG 335
           LAA +AA+ DGVDV+SIS+G        +  D +++G++ A  +GI V  S GN+GP   
Sbjct: 275 LAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYGAASKGIFVSSSAGNEGPNGM 334

Query: 336 TVTNVSPWMFTVAASTIDRDFVNYVALGNKRHFEGVSLSSGGLPRGKFYPLVDGVQVKAA 395
           +VTN++PW+ TV ASTIDR+F     LG+     GVSL +G    G+ +P+V   +   +
Sbjct: 335 SVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYAGVPLNGRMFPVVYPGKSGMS 394

Query: 396 NATDNLALLCEDGSLDPVKAKGKIVLCLRGDSARMDKSFEVRRAGGVGLILVNDKQDGND 455
           +A+     LC + +LDP + +GKIV+C RG S R+ K   V++AGGVG+IL N   +G  
Sbjct: 395 SAS-----LCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKAGGVGMILANGASNGEG 454

Query: 456 ITADPHFLPASHLNYADGLAIFQYVNSTKSPMAFITHVKTELGIKPSPMVADFSSRGPNP 515
           +  D H +PA  +   +G  I  Y +S  +P+A I    T +GIKP+P++A FS RGPN 
Sbjct: 455 LVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVGIKPAPVIASFSGRGPNG 514

Query: 516 IIDSMIKPDIAAPGVSILAAFSKYVTATDFPLDTRRVPFNFESGTSMACPHISGVVGLLK 575
           +   ++KPD+ APGV+ILAA++  V  T  P D R+  FN  SGTSMACPH+SG   LLK
Sbjct: 515 LSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLK 574

Query: 576 TLYPNWSPAAIKSAIMTTAKTRDNSMKSILDYNKVK-ATPFQYGAGHVHPNNAIDPGLVY 635
           + +P+WSPA I+SA+MTT    DNS +S++D +  K ATP+ YG+GH++   A++PGLVY
Sbjct: 575 SAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSATPYDYGSGHLNLGRAMNPGLVY 634

Query: 636 DTTIEDYMNFICAQGYNSTTLKKFYNKPFLCP--KSFALADLNYPSISV--PKLTAG-VP 695
           D T +DY+ F+C+ GY   T++     P  CP  +  +  +LNYPSI+   P    G V 
Sbjct: 635 DITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVS 694

Query: 696 ATINRRLKNVG-TPGTYVARVKVSSKVSVTVKPSTLQFNSVGEEKAFKVVFEYKGKGI-- 755
            T+ R   NVG     Y AR++    V+VTVKP  L F S  + +++ V      + +  
Sbjct: 695 KTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVL 754

Query: 756 -GKGHVFGTLIWSD-GKHFVRSPMAV 762
              G VFG++ W D GKH VRSP+ V
Sbjct: 755 GETGAVFGSVTWFDGGKHVVRSPIVV 758