PI0002153 (gene) Melon (PI 482460) v1

Overview
NamePI0002153
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionMechanosensitive ion channel protein
Locationchr02: 2471372 .. 2474018 (+)
RNA-Seq ExpressionPI0002153
SyntenyPI0002153
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGATAAGAAAGGAATGGAACAGCTTGTTTTACGGATTTTGGAAGGTGAAGAAGGAGTTCATGGAAGTAGTAAAGATCTTAATAAACCCTCTGTTGGTTCTTTTCCTGATTTTGACCTAAAAGAAACTCGGAGTTTTAGGTGCACGATTCCACAATCCGTGGTTGGGAGTTCTCCTTCACATGAGATTTCCAGAATGACTCCTCTTAAACCTCCAAAAATTCCTGGCGAAACGGTGACACGGCGTGCATCATTTGCTTGTTCGTCGTTTTCGAAGCCGAAATCGAGGCTAATAGAGTCTCCTTGTCCTGATGGTGCAAGTTTAGCAGAAGAAAAGGCTCTAGCAAAATCAACATTGTATAGCTCTCCTAAGGTGGACTCTCCAGCTAAGATAACCACTGTGACGAGTCCTAAAGAAGCTTTGAAGTCGACCCCGATAACTCCGAAAACGCCGTTGATTGGAACTACTGGGAATGAGGAGGAAGATGATGAAGAAGTTTACAAAACTGCAGAACTGAAAGTGAAAGAAAAATCTGGGAAGAGATTGAAGAAAACAGTTTTAATGGAATGGGTTGCATTTTTGTGCTTAACAGGGTGTTTGATTGCTAGCTTAACTATAGACAAGTTGGTGACCAAAGAGATTTGGGGATTAGGACTGTGGAAATGGTGCGTTCTTGTATTAGTTATTTTCTGTGGTCGTTTGTTTTCGCAATGGTTTATCAATTGTCTGGTTTTCTTGATTGAAAGAAACTTTCTACTTAAAAGAAAGGTTCTTTATTTTGTCTATGGGCTGAAGAAGAGTGTTATAATTTTTATTTGGCTGGCTTTAGTTCTTCTAGCCTGGGGTCTATTATTTGATCAAAGCAGCAAGAGATCTAAGAAAGGCAACGAGATTCTGAATTACGTTACACGAGCTCTTGGTGCTTCTCTAATTGGAGCAGGACTATGGCTGGTTAAAACTTTGTTGGTGAAGATACTCGCTGCTTCTTTTCAGTGCACTAGATTCTTTGATCGGATTCAAGAATCAATCTTCCATCAATATATACTACGCATCCTATCAGGACCTCCGCTCATGGAGATGGCTGAGAGGGTCGGGAGAGCGGCAAGCACAGGACAGTTGAGTTTCAGGCATTTGAAGAAAGAAAGTGATGGTGGGAATGAAGGGAAGGAAGAGGTTATTGATGTAGATAAACTCAAAAAGATGAAGCAAGAAAAAATCTCTGCTTGGACCATGAGAGGGCTAATCAATGTTATAAGGGGTTCAGGATTGTCCACCATCTCTAATACAATAGAGAATTTTAAAGAGGAAGAGGTCGAGCAAAAGGATAAGGAGATTAACAGTGAATGGGAAGCAAGGGCTGCAGCTTACCAGATTTTCCGAAACGTTGCAAAACCGGGTAGCAAGTAAGAATTTTACCTTCTGAAAGATCTTGAATTTCACACTTCGTGATATTTGGAGAAATTTGTTAACCAATACTTTTAATCTTGTTCCCATGGTTAGGTATATTGACGAAGAGGACCTCTTTCGTTTTATGAGTAAGGAGGAAATTGATAATGTGCTGCCATTGTTTGAAGGAGGAGCTGAAACTGGGAAGATAAAGCGAAAAACCCTGAAGAATTGGCTGGTAACATTTTAGCATTTTCTTTCGAAGTGAAATTCTAAGCAAACCTCTTACAAACCTATGCCTTAGAAATGTATTTTTGATGTTGCAGGTGAATGTTTACGTCGAACGCAAGTCATTAGCTCACTCATTGAATGACACCAAGACTGCCATAGAGGAGCTAAACAAGCTTGCTTCTGCAGTTGTACTGATTGTGATTATCATTGAATGGCTACTTCTGATGGGTTTCTTAACTACGCAAGTACTCGTCTTCATTTCATCACAGATTCTATTGGTGGTTTTCATGTTCGGTAACACTGCCAGAACTGTATTTGAAGCCATCATATTCGTATTCGTGATGCATCCATTCGATGTTGGGGATCGTTGTGTCGTAGATGGTGTCCAGGTAACTTTCTTAATTCATAATGTTTTTGGATTCTATCGAAGCAATCTTACCTGGTTTAATTCTAACTCATGCAGATGATTGTTGAAGAAATGAACATTTTAACCACAATCTTCTTGAGATATGATAACGAGAAGATCTTCTATCCAAATTCTGTTCTAGCCACCAAACCCATTAGTAACTACTACAGGAGCCCCGAAATGAGCGACTCAATTGATTTTTCTGTTGACTTTTCCACCTCAATCGAAAGCATCGGAGCTCTAAAAGCAAGAATAAAAACGTAAGTTCCATTCAGTTTCTCTATGCTTGTTAGTAATAAACTACAACAGCATTGAGTTTGACATATCATTTCAAATGTATTGCTCAGATACTTAGAAAGCAAACCGCAGTTCTGGCGACCGAACTACAGTGTCGTTGTGAAAGAAATCGAGAATGTCGACAAAATGAAACTATCTCTATGTGTTAATCACACCATCAACTTTCAGAACTACGGCGACAAGAGCAATCGTAGATCGGATTTGGTCTTGGAGCTGAAGAAAATTTTCGAAGATCTCGGTATCAAGTATCATCTGTTACCTCAAGAAGTTCAGCTCAACTATGTGAGCTCAGCAGCTGGTGTGGTTCCACCCTCTCAAAGATGA

mRNA sequence

ATGGCGGATAAGAAAGGAATGGAACAGCTTGTTTTACGGATTTTGGAAGGTGAAGAAGGAGTTCATGGAAGTAGTAAAGATCTTAATAAACCCTCTGTTGGTTCTTTTCCTGATTTTGACCTAAAAGAAACTCGGAGTTTTAGGTGCACGATTCCACAATCCGTGGTTGGGAGTTCTCCTTCACATGAGATTTCCAGAATGACTCCTCTTAAACCTCCAAAAATTCCTGGCGAAACGGTGACACGGCGTGCATCATTTGCTTGTTCGTCGTTTTCGAAGCCGAAATCGAGGCTAATAGAGTCTCCTTGTCCTGATGGTGCAAGTTTAGCAGAAGAAAAGGCTCTAGCAAAATCAACATTGTATAGCTCTCCTAAGGTGGACTCTCCAGCTAAGATAACCACTGTGACGAGTCCTAAAGAAGCTTTGAAGTCGACCCCGATAACTCCGAAAACGCCGTTGATTGGAACTACTGGGAATGAGGAGGAAGATGATGAAGAAGTTTACAAAACTGCAGAACTGAAAGTGAAAGAAAAATCTGGGAAGAGATTGAAGAAAACAGTTTTAATGGAATGGGTTGCATTTTTGTGCTTAACAGGGTGTTTGATTGCTAGCTTAACTATAGACAAGTTGGTGACCAAAGAGATTTGGGGATTAGGACTGTGGAAATGGTGCGTTCTTGTATTAGTTATTTTCTGTGGTCGTTTGTTTTCGCAATGGTTTATCAATTGTCTGGTTTTCTTGATTGAAAGAAACTTTCTACTTAAAAGAAAGGTTCTTTATTTTGTCTATGGGCTGAAGAAGAGTGTTATAATTTTTATTTGGCTGGCTTTAGTTCTTCTAGCCTGGGGTCTATTATTTGATCAAAGCAGCAAGAGATCTAAGAAAGGCAACGAGATTCTGAATTACGTTACACGAGCTCTTGGTGCTTCTCTAATTGGAGCAGGACTATGGCTGGTTAAAACTTTGTTGGTGAAGATACTCGCTGCTTCTTTTCAGTGCACTAGATTCTTTGATCGGATTCAAGAATCAATCTTCCATCAATATATACTACGCATCCTATCAGGACCTCCGCTCATGGAGATGGCTGAGAGGGTCGGGAGAGCGGCAAGCACAGGACAGTTGAGTTTCAGGCATTTGAAGAAAGAAAGTGATGGTGGGAATGAAGGGAAGGAAGAGGTTATTGATGTAGATAAACTCAAAAAGATGAAGCAAGAAAAAATCTCTGCTTGGACCATGAGAGGGCTAATCAATGTTATAAGGGGTTCAGGATTGTCCACCATCTCTAATACAATAGAGAATTTTAAAGAGGAAGAGGTCGAGCAAAAGGATAAGGAGATTAACAGTGAATGGGAAGCAAGGGCTGCAGCTTACCAGATTTTCCGAAACGTTGCAAAACCGGGTAGCAAGTATATTGACGAAGAGGACCTCTTTCGTTTTATGAGTAAGGAGGAAATTGATAATGTGCTGCCATTGTTTGAAGGAGGAGCTGAAACTGGGAAGATAAAGCGAAAAACCCTGAAGAATTGGCTGGTGAATGTTTACGTCGAACGCAAGTCATTAGCTCACTCATTGAATGACACCAAGACTGCCATAGAGGAGCTAAACAAGCTTGCTTCTGCAGTTGTACTGATTGTGATTATCATTGAATGGCTACTTCTGATGGGTTTCTTAACTACGCAAGTACTCGTCTTCATTTCATCACAGATTCTATTGGTGGTTTTCATGTTCGGTAACACTGCCAGAACTGTATTTGAAGCCATCATATTCGTATTCGTGATGCATCCATTCGATGTTGGGGATCGTTGTGTCGTAGATGGTGTCCAGATGATTGTTGAAGAAATGAACATTTTAACCACAATCTTCTTGAGATATGATAACGAGAAGATCTTCTATCCAAATTCTGTTCTAGCCACCAAACCCATTAGTAACTACTACAGGAGCCCCGAAATGAGCGACTCAATTGATTTTTCTGTTGACTTTTCCACCTCAATCGAAAGCATCGGAGCTCTAAAAGCAAGAATAAAAACATACTTAGAAAGCAAACCGCAGTTCTGGCGACCGAACTACAGTGTCGTTGTGAAAGAAATCGAGAATGTCGACAAAATGAAACTATCTCTATGTGTTAATCACACCATCAACTTTCAGAACTACGGCGACAAGAGCAATCGTAGATCGGATTTGGTCTTGGAGCTGAAGAAAATTTTCGAAGATCTCGGTATCAAGTATCATCTGTTACCTCAAGAAGTTCAGCTCAACTATGTGAGCTCAGCAGCTGGTGTGGTTCCACCCTCTCAAAGATGA

Coding sequence (CDS)

ATGGCGGATAAGAAAGGAATGGAACAGCTTGTTTTACGGATTTTGGAAGGTGAAGAAGGAGTTCATGGAAGTAGTAAAGATCTTAATAAACCCTCTGTTGGTTCTTTTCCTGATTTTGACCTAAAAGAAACTCGGAGTTTTAGGTGCACGATTCCACAATCCGTGGTTGGGAGTTCTCCTTCACATGAGATTTCCAGAATGACTCCTCTTAAACCTCCAAAAATTCCTGGCGAAACGGTGACACGGCGTGCATCATTTGCTTGTTCGTCGTTTTCGAAGCCGAAATCGAGGCTAATAGAGTCTCCTTGTCCTGATGGTGCAAGTTTAGCAGAAGAAAAGGCTCTAGCAAAATCAACATTGTATAGCTCTCCTAAGGTGGACTCTCCAGCTAAGATAACCACTGTGACGAGTCCTAAAGAAGCTTTGAAGTCGACCCCGATAACTCCGAAAACGCCGTTGATTGGAACTACTGGGAATGAGGAGGAAGATGATGAAGAAGTTTACAAAACTGCAGAACTGAAAGTGAAAGAAAAATCTGGGAAGAGATTGAAGAAAACAGTTTTAATGGAATGGGTTGCATTTTTGTGCTTAACAGGGTGTTTGATTGCTAGCTTAACTATAGACAAGTTGGTGACCAAAGAGATTTGGGGATTAGGACTGTGGAAATGGTGCGTTCTTGTATTAGTTATTTTCTGTGGTCGTTTGTTTTCGCAATGGTTTATCAATTGTCTGGTTTTCTTGATTGAAAGAAACTTTCTACTTAAAAGAAAGGTTCTTTATTTTGTCTATGGGCTGAAGAAGAGTGTTATAATTTTTATTTGGCTGGCTTTAGTTCTTCTAGCCTGGGGTCTATTATTTGATCAAAGCAGCAAGAGATCTAAGAAAGGCAACGAGATTCTGAATTACGTTACACGAGCTCTTGGTGCTTCTCTAATTGGAGCAGGACTATGGCTGGTTAAAACTTTGTTGGTGAAGATACTCGCTGCTTCTTTTCAGTGCACTAGATTCTTTGATCGGATTCAAGAATCAATCTTCCATCAATATATACTACGCATCCTATCAGGACCTCCGCTCATGGAGATGGCTGAGAGGGTCGGGAGAGCGGCAAGCACAGGACAGTTGAGTTTCAGGCATTTGAAGAAAGAAAGTGATGGTGGGAATGAAGGGAAGGAAGAGGTTATTGATGTAGATAAACTCAAAAAGATGAAGCAAGAAAAAATCTCTGCTTGGACCATGAGAGGGCTAATCAATGTTATAAGGGGTTCAGGATTGTCCACCATCTCTAATACAATAGAGAATTTTAAAGAGGAAGAGGTCGAGCAAAAGGATAAGGAGATTAACAGTGAATGGGAAGCAAGGGCTGCAGCTTACCAGATTTTCCGAAACGTTGCAAAACCGGGTAGCAAGTATATTGACGAAGAGGACCTCTTTCGTTTTATGAGTAAGGAGGAAATTGATAATGTGCTGCCATTGTTTGAAGGAGGAGCTGAAACTGGGAAGATAAAGCGAAAAACCCTGAAGAATTGGCTGGTGAATGTTTACGTCGAACGCAAGTCATTAGCTCACTCATTGAATGACACCAAGACTGCCATAGAGGAGCTAAACAAGCTTGCTTCTGCAGTTGTACTGATTGTGATTATCATTGAATGGCTACTTCTGATGGGTTTCTTAACTACGCAAGTACTCGTCTTCATTTCATCACAGATTCTATTGGTGGTTTTCATGTTCGGTAACACTGCCAGAACTGTATTTGAAGCCATCATATTCGTATTCGTGATGCATCCATTCGATGTTGGGGATCGTTGTGTCGTAGATGGTGTCCAGATGATTGTTGAAGAAATGAACATTTTAACCACAATCTTCTTGAGATATGATAACGAGAAGATCTTCTATCCAAATTCTGTTCTAGCCACCAAACCCATTAGTAACTACTACAGGAGCCCCGAAATGAGCGACTCAATTGATTTTTCTGTTGACTTTTCCACCTCAATCGAAAGCATCGGAGCTCTAAAAGCAAGAATAAAAACATACTTAGAAAGCAAACCGCAGTTCTGGCGACCGAACTACAGTGTCGTTGTGAAAGAAATCGAGAATGTCGACAAAATGAAACTATCTCTATGTGTTAATCACACCATCAACTTTCAGAACTACGGCGACAAGAGCAATCGTAGATCGGATTTGGTCTTGGAGCTGAAGAAAATTTTCGAAGATCTCGGTATCAAGTATCATCTGTTACCTCAAGAAGTTCAGCTCAACTATGTGAGCTCAGCAGCTGGTGTGGTTCCACCCTCTCAAAGATGA

Protein sequence

MADKKGMEQLVLRILEGEEGVHGSSKDLNKPSVGSFPDFDLKETRSFRCTIPQSVVGSSPSHEISRMTPLKPPKIPGETVTRRASFACSSFSKPKSRLIESPCPDGASLAEEKALAKSTLYSSPKVDSPAKITTVTSPKEALKSTPITPKTPLIGTTGNEEEDDEEVYKTAELKVKEKSGKRLKKTVLMEWVAFLCLTGCLIASLTIDKLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWFINCLVFLIERNFLLKRKVLYFVYGLKKSVIIFIWLALVLLAWGLLFDQSSKRSKKGNEILNYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLMEMAERVGRAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIRGSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFMSKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASAVVLIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDFSTSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVDKMKLSLCVNHTINFQNYGDKSNRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVSSAAGVVPPSQR
Homology
BLAST of PI0002153 vs. ExPASy Swiss-Prot
Match: Q9LYG9 (Mechanosensitive ion channel protein 10 OS=Arabidopsis thaliana OX=3702 GN=MSL10 PE=1 SV=1)

HSP 1 Score: 675.2 bits (1741), Expect = 8.4e-193
Identity = 371/717 (51.74%), Postives = 504/717 (70.29%), Query Frame = 0

Query: 45  RSFRCTIPQSVVG----SSPSHEISRM--TPLKPPKIPGET---VTRRASFACSSFSKPK 104
           RS     P+S  G     SPS EIS++  +P KPP+ P +    +T+R SFA S +SKPK
Sbjct: 28  RSKEMASPESEKGVPFSKSPSPEISKLVGSPNKPPRAPNQNNVGLTQRKSFARSVYSKPK 87

Query: 105 SRLIESPCPDGASLAEEKALAKSTLYSSPKVDSPAKITTVTSPKEALKSTPITPKTPLIG 164
           SR ++  CP   S+ EE+   +     S    SP       S +      P+TP   ++ 
Sbjct: 88  SRFVDPSCPVDTSILEEEVREQLGAGFSFSRASPNN----KSNRSVGSPAPVTPSKVVV- 147

Query: 165 TTGNEEEDDEEVYKTAELKVKEKSGKRLKKTVLMEWVAFLCLTGCLIASLTIDKLVTKEI 224
               E+++DEE+YK  ++K+  +   ++    L+E   F+ +   L+ASLTI+ L     
Sbjct: 148 ----EKDEDEEIYK--KVKLNREMRSKISTLALIESAFFVVILSALVASLTINVLKHHTF 207

Query: 225 WGLGLWKWCVLVLVIFCGRLFSQWFINCLVFLIERNFLLKRKVLYFVYGLKKSVIIFIWL 284
           WGL +WKWCVLV+VIF G L + WF+  +VFLIE NFLL+RKVLYFV+GLKKSV +FIWL
Sbjct: 208 WGLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLIETNFLLRRKVLYFVHGLKKSVQVFIWL 267

Query: 285 ALVLLAWGLLFDQSSKRSKKGNEILNYVTRALGASLIGAGLWLVKTLLVKILAASFQCTR 344
            L+L+AW LLF+   KRS    ++L  +TR L + L GA  WLVKTLL+KILAA+F    
Sbjct: 268 CLILVAWILLFNHDVKRSPAATKVLKCITRTLISILTGAFFWLVKTLLLKILAANFNVNN 327

Query: 345 FFDRIQESIFHQYILRILSGPPLMEMAERVGRAASTGQLSFRHLKKESDGGNEGKEEVID 404
           FFDRIQ+S+FHQY+L+ LSG PLME AERVGR  STG LSF  + K+   G   +++VID
Sbjct: 328 FFDRIQDSVFHQYVLQTLSGLPLMEEAERVGREPSTGHLSFATVVKK---GTVKEKKVID 387

Query: 405 VDKLKKMKQEKISAWTMRGLINVIRGSGLSTISNTI-ENFKEEEVEQKDKEINSEWEARA 464
           + K+ KMK+EK+SAWTMR L+  +R SGLSTIS+T+ E    E  EQ D+EI SE EA A
Sbjct: 388 MGKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDTLDETAYGEGKEQADREITSEMEALA 447

Query: 465 AAYQIFRNVAKPGSKYIDEEDLFRFMSKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVY 524
           AAY +FRNVA+P   YI+EEDL RFM KEE+D V PLF+G AETG+I RK    W+V VY
Sbjct: 448 AAYHVFRNVAQPFFNYIEEEDLLRFMIKEEVDLVFPLFDGAAETGRITRKAFTEWVVKVY 507

Query: 525 VERKSLAHSLNDTKTAIEELNKLASAVVLIVIIIEWLLLMGFLTTQVLVFISSQILLVVF 584
             R++LAHSLNDTKTA+++LNKL +A++++V ++ WLLL+   TT+VL+F S+Q++ + F
Sbjct: 508 TSRRALAHSLNDTKTAVKQLNKLVTAILMVVTVVIWLLLLEVATTKVLLFFSTQLVALAF 567

Query: 585 MFGNTARTVFEAIIFVFVMHPFDVGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNS 644
           + G+T + +FE+I+FVFVMHP+DVGDRCVVDGV M+VEEMN+LTT+FL+ +NEK++YPN+
Sbjct: 568 IIGSTCKNLFESIVFVFVMHPYDVGDRCVVDGVAMLVEEMNLLTTVFLKLNNEKVYYPNA 627

Query: 645 VLATKPISNYYRSPEMSDSIDFSVDFSTSIESIGALKARIKTYLESKPQFWRPNYSVVVK 704
           VLATKPISNY+RSP M ++++FS+ FST +  I  LK RI  YLE  PQ W P +SVVVK
Sbjct: 628 VLATKPISNYFRSPNMGETVEFSISFSTPVSKIAHLKERIAEYLEQNPQHWAPVHSVVVK 687

Query: 705 EIENVDKMKLSLCVNHTINFQNYGDKSNRRSDLVLELKKIFEDLGIKYHLLPQEVQL 752
           EIEN++K+K++L  +HTI FQ   +++ RR++L L +K++ EDL I Y LLPQ++ L
Sbjct: 688 EIENMNKLKMALYSDHTITFQENRERNLRRTELSLAIKRMLEDLHIDYTLLPQDINL 730

BLAST of PI0002153 vs. ExPASy Swiss-Prot
Match: Q84M97 (Mechanosensitive ion channel protein 9 OS=Arabidopsis thaliana OX=3702 GN=MSL9 PE=1 SV=1)

HSP 1 Score: 610.5 bits (1573), Expect = 2.6e-173
Identity = 355/752 (47.21%), Postives = 483/752 (64.23%), Query Frame = 0

Query: 13  RILEGEEGVHGSSKDLNKPSVGSFPDFDLKETRSFRCTIPQSVVG---SSPSHEISRMTP 72
           R+  GEE V   S   +     + P F+           P S  G   S P   IS  TP
Sbjct: 5   RVSNGEEVVINVSDKEDSKDPRASPSFN-------PLASPDSDAGIEKSKPVPPISIPTP 64

Query: 73  ---------LKPPKIPG-ETVTRRASFACSSFSKPKSRLIESPCPDGASLAEEKALAKST 132
                     KPPKIP  E + RR S + S +SKPKSR  E       S  EE    +S 
Sbjct: 65  EIYKFSGSVHKPPKIPSPEGLVRRKSLSRSIYSKPKSRFGEQQSFRYDSTREENG-GRSL 124

Query: 133 LYSSPKVDSPAKITTVTSPKEALKSTPITPKTPLIGTTGNEEEDDEEVYKTAELKVKEKS 192
                            SP    KS        L      E +++EE+YK  +L   ++S
Sbjct: 125 REQFGAGSFARGSFDRASPNN--KSNRSVASAALSKVAEEEPDENEEIYKKVKLHRVKRS 184

Query: 193 GKRLKKTVLMEWVAFLCLTGCLIASLTIDKLVTKEIWGLGLWKWCVLVLVIFCGRLFSQW 252
           G  +K    +E V F+ + G LI SLTID +    IWGL  WKWCVLV+V   G L + W
Sbjct: 185 G--MKPLAFLELVVFMAILGALIVSLTIDVVNKHTIWGLEFWKWCVLVMVTLSGMLVTNW 244

Query: 253 FINCLVFLIERNFLLKRKVLYFVYGLKKSVIIFIWLALVLLAWGLLFDQSSKRSKKGNEI 312
           F++ +VF+IE+N+LL++KVLYFV+GLKK+V +FIW +LVL+AW  LFD   KR++K    
Sbjct: 245 FMHFVVFIIEKNYLLRKKVLYFVHGLKKNVQVFIWFSLVLIAWICLFDGDVKRTRKTKRF 304

Query: 313 LNYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLM 372
           L+++T  + + L+G+ L+LVKT  +K+LA+ F    FF+RIQES+FHQY+L+ LSGPPL+
Sbjct: 305 LDFITWTIVSLLVGSILFLVKTFALKVLASKFNVRNFFERIQESVFHQYVLQTLSGPPLI 364

Query: 373 EMAERVGRAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVI 432
           E AE VGR  STG LSF   K     G    ++VID+ K+ +MKQEK+SAWTMR LI  +
Sbjct: 365 EEAENVGRVPSTGHLSFTRTK----DGKVKDKKVIDMGKVHRMKQEKVSAWTMRVLIEAV 424

Query: 433 RGSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRF 492
             SG+STIS+T++    ++ E+ DKEI +E EA AAAY +F NVAKP   YI+E+DL RF
Sbjct: 425 GTSGISTISSTLDEVNNKK-ERTDKEITNEMEAVAAAYDVFNNVAKPNHNYIEEDDLLRF 484

Query: 493 MSKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLAS 552
           M KEE+D VLPL E  A+TGKI RKT   W+VNVY  RK++ HSLNDTKTA+++L+KL +
Sbjct: 485 MIKEEVDLVLPLIE-DADTGKITRKTFTEWVVNVYTSRKTIGHSLNDTKTAVKQLDKLIT 544

Query: 553 AVVLIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVG 612
            ++ ++  I W++L+   +T++L+  SSQ L + FM G+T + +FE+ +FVFVMHP+DVG
Sbjct: 545 GILTVITFIVWMVLLDIASTKLLLVFSSQFLGLAFMIGSTCKNIFESFMFVFVMHPYDVG 604

Query: 613 DRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVD 672
           DRCVVDGV ++VEE+++LTT+FL+ DNEK+FYPNSVL +KPISN+YRSP+M D +DF + 
Sbjct: 605 DRCVVDGVMLLVEEIDLLTTVFLKIDNEKVFYPNSVLISKPISNFYRSPDMGDYVDFGIA 664

Query: 673 FSTSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVDKMKLSLCVNHTINFQNYGD 732
           FST  E IG LK +I  YL +  Q W P   V+V+ IEN++K+ L++ V HTINFQ Y +
Sbjct: 665 FSTPAEKIGCLKGKIGEYLVANSQHWYPEAQVMVRAIENMNKLVLNILVQHTINFQVYVE 724

Query: 733 KSNRRSDLVLELKKIFEDLGIKYHLLPQEVQL 752
           KS RR+ L++ +K+I EDL I Y LLPQ+V L
Sbjct: 725 KSLRRTALIIAIKRILEDLEIDYTLLPQDVNL 738

BLAST of PI0002153 vs. ExPASy Swiss-Prot
Match: Q9LPG3 (Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana OX=3702 GN=MSL4 PE=3 SV=1)

HSP 1 Score: 516.2 bits (1328), Expect = 6.5e-145
Identity = 314/790 (39.75%), Postives = 466/790 (58.99%), Query Frame = 0

Query: 21  VHGSSKDLNKPSVGSFPD--FDLKETRSFRCTIPQSVV-GSSPSHEISRMTPLKPPKIPG 80
           V GS+  +  P   S+ +   D    R  R T+  SV  G S   E +R+   +  K   
Sbjct: 112 VSGSASPVTAPVTASYRNGTGDAIRRRQNRVTLSPSVKDGDSSEDEENRVDGSEVVKCTS 171

Query: 81  ETVTRRASFACSSFSKPKSRLIESPCPDGASLAEEKALAKSTLYSSPKVDSPAKITTVTS 140
              T R         K +SRL++ P P    +   +      L                +
Sbjct: 172 NRSTMRTKTLMK--MKTRSRLMDPPTPTYPDMVSGRTPRSGNL----------------N 231

Query: 141 PKEALKSTPITPKTPLIGTTGNEEEDDEEVYKTAELKVKEKSGKRLKKT---VLMEWVAF 200
           P  + ++T   P TP  G +  + E++E+ +   +L      G R +K    V++EW+  
Sbjct: 232 PGFSGRNT--KPGTPNQGGS-KDLEEEEDPFSEEDL----PEGLRKEKICVWVIIEWIFL 291

Query: 201 LCLTGCLIASLTIDKLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWFINCLVFLIERNFLL 260
           + +   LI SL I  L  K +W L LWKW V+VLV+ CGRL S W +   V+ +E NFL 
Sbjct: 292 ILIIASLICSLVIPYLRGKTLWDLALWKWEVMVLVLICGRLVSSWIVKLFVYFVESNFLW 351

Query: 261 KRKVLYFVYGLKKSVIIFIWLALVLLAWGLLFDQSSKRSKKGNEILNYVTRALGASLIGA 320
           ++KVLYFVYG++K V   +WL LVL+AW  LFD+  +R  + + +L YVT+ L   L+  
Sbjct: 352 RKKVLYFVYGIRKPVQNCLWLGLVLIAWHFLFDKKVEREMR-STVLKYVTKVLICLLVAV 411

Query: 321 GLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLMEM---AERVGRAAST 380
            +WL+KTLLVK+LA+SF  + +FDRIQES+F QY++  LSGPP +E+    E+V     T
Sbjct: 412 IIWLIKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPRIEIHIEEEKVANDVKT 471

Query: 381 GQLSFRHLK---------------------------------KESDGGNEGKEEVIDVDK 440
            ++  R L                                    S    EG EE I +D 
Sbjct: 472 FEIVGRKLSPLGPKAVSSPPQVTVGSGRLQKSPSRVGKSPVLSRSGSKKEGGEEGIRIDH 531

Query: 441 LKKMKQEKISAWTMRGLINVIRGSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQ 500
           L++M  + +SAW M+ L+NVI+   LST+   I++   +E + K  +I SE+EA+ AA +
Sbjct: 532 LQRMNTKNVSAWKMKKLMNVIKKGTLSTLDEQIQDTTTQE-DDKATQIRSEFEAKLAARK 591

Query: 501 IFRNVAKPGSKYIDEEDLFRFMSKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERK 560
           IF+NVA+PGS+YI  ED  RF+S++E +  + LFEG +E  KI +  LKNW+VN + ER+
Sbjct: 592 IFQNVAEPGSRYIYMEDFMRFLSEDESERAMDLFEGASECHKISKSCLKNWVVNAFRERR 651

Query: 561 SLAHSLNDTKTAIEELNKLASAVVLIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGN 620
           +LA +LNDTKTA+  L+++   +V IVI+I WLL++G  TT+ L+ ISSQ+LLVVF+FGN
Sbjct: 652 ALALTLNDTKTAVNRLHRIVDVLVSIVILIIWLLILGIATTKFLLVISSQLLLVVFVFGN 711

Query: 621 TARTVFEAIIFVFVMHPFDVGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLAT 680
           + +T+FEA+IFVFVMHPFDVGDRC +DGVQMIVEEMNILTT+FLR+DN+KI YPNS+L T
Sbjct: 712 SCKTIFEAVIFVFVMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQKIVYPNSLLGT 771

Query: 681 KPISNYYRSPEMSDSIDFSVDFSTSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIEN 740
           KPI+NYYRSP+M D+I+F V  +T  E   AL+ RI +Y+++K   W P+  +V +++  
Sbjct: 772 KPIANYYRSPDMQDAIEFFVHIATPPEKTTALRQRILSYVDNKKDHWHPSPMIVFRDMCG 831

Query: 741 VDKMKLSLCVNHTINFQNYGDKSNRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVSSAA 765
           ++ +K+++   H +N QN G++  RR  L+ E+ ++  +L I+Y L P  + +  + +A 
Sbjct: 832 LNSVKIAMWPTHKMNHQNMGERYVRRGQLLEEIGRLCRELDIEYRLYPLNINVKSLPAAT 874

BLAST of PI0002153 vs. ExPASy Swiss-Prot
Match: Q9LH74 (Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana OX=3702 GN=MSL5 PE=2 SV=1)

HSP 1 Score: 516.2 bits (1328), Expect = 6.5e-145
Identity = 296/708 (41.81%), Postives = 441/708 (62.29%), Query Frame = 0

Query: 90  SFSKPKSRLIESPCPDGASL--AEEKALAKSTLYSSPKVDSPAKITTVTSPKEALKSTPI 149
           S +K KSRL + P P   ++   E K+  +S ++ S  +                     
Sbjct: 190 SRNKTKSRLQDPPTPTHPAIDKTEMKSGRRSGIFKSGFLGK------------------- 249

Query: 150 TPKTPLIGTTGNEEEDDEEVYKTAELKVKEKSGKRLKKTVLMEWVAFLCLTGCLIASLTI 209
           +PK    G  G EEE++E+ +   +L  + K  K L   V +EW++ + +   L+ SLTI
Sbjct: 250 SPKAGTPGRNGFEEEEEEDPFLDEDLPEEFKRDK-LSFWVFLEWISLVLIVTSLVCSLTI 309

Query: 210 DKLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWFINCLVFLIERNFLLKRKVLYFVYGLKK 269
             L  K  W L LWKW V VLV+ CGRL S W +  +VFL+E+NF  +++VLYFVYG++K
Sbjct: 310 HNLQRKTWWKLDLWKWEVTVLVLICGRLVSSWIVRIIVFLVEKNFTWRKRVLYFVYGVRK 369

Query: 270 SVIIFIWLALVLLAWGLLFDQSSKRSKKGNEILNYVTRALGASLIGAGLWLVKTLLVKIL 329
           SV   +WL LVLLAW  LFD+  +R  + +  L YVTR L   L+   +WLVKT+LVK+L
Sbjct: 370 SVQNCLWLGLVLLAWHFLFDKKVERETR-STALRYVTRVLVCLLVALIIWLVKTILVKVL 429

Query: 330 AASFQCTRFFDRIQESIFHQYILRILSGPPLMEM---------------------AERVG 389
           A+SF  + +FDRIQES+F QY++  LSGPPLME+                       ++ 
Sbjct: 430 ASSFHMSTYFDRIQESLFTQYVIETLSGPPLMEIQRMEEEEQQVAEDVKSLEKLAGAKLP 489

Query: 390 RAASTGQLSFRHLKKESD----GGNEGKE-EVIDVDKLKKMKQEKISAWTMRGLINVIRG 449
            A      SF  + K       G   G++ E I +D+LK+M  + +SAW M+ L+N+I  
Sbjct: 490 PALKATVKSFMKVGKSPGLNRIGSKRGEDGEGIRIDQLKRMNTKNVSAWNMKRLMNIILK 549

Query: 450 SGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFMS 509
             +ST+   +++  +E  ++    I SE+EA+ AA +IF NV +PGS+YI  ED  RF+ 
Sbjct: 550 GAISTLDQNMQDTTQE--DEDATHIRSEYEAKCAARKIFHNVTEPGSRYIYLEDFLRFLC 609

Query: 510 KEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASAV 569
           +EE +  + LFEG +E+ KI +  LKNW+V  + ER++LA +LNDTKTA++ L+++ + V
Sbjct: 610 EEEAERAMALFEGASESDKISKSCLKNWVVKAFRERRALALTLNDTKTAVDRLHRIINVV 669

Query: 570 VLIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGDR 629
           + I+III WLL++G  TT+ L+ +SSQ+LLV F+FGN+ +T+FEAIIF+FVMHPFDVGDR
Sbjct: 670 IGIIIIIIWLLILGIATTRFLLVLSSQLLLVAFVFGNSCKTIFEAIIFLFVMHPFDVGDR 729

Query: 630 CVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDFS 689
           C +DGVQ++VEEMNILTT+FLRYDN+KI YPNSVL TKPI+NYYRSP+M D+++F V  +
Sbjct: 730 CEIDGVQLVVEEMNILTTVFLRYDNQKIIYPNSVLGTKPIANYYRSPDMGDAVEFCVHIA 789

Query: 690 TSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVDKMKLSLCVNHTINFQNYGDKS 749
           T  E I A+K RI +Y+++K  +W P   +V   +++++ +K+++ + H +N Q+ G++ 
Sbjct: 790 TPPEKITAIKQRILSYVDNKKDYWYPAPMIVFLSMDDLNSVKIAVWLTHRMNHQDMGERY 849

Query: 750 NRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVSSAAGV-----VPPS 765
            RR  L+ E+ K   +L I+Y L P  + +  +   A       +PPS
Sbjct: 850 IRRGLLLEEVGKTCRELDIEYRLYPLNINVRSLPPTANPTSSDRIPPS 874

BLAST of PI0002153 vs. ExPASy Swiss-Prot
Match: Q9SYM1 (Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana OX=3702 GN=MSL6 PE=1 SV=1)

HSP 1 Score: 505.4 bits (1300), Expect = 1.2e-141
Identity = 290/684 (42.40%), Postives = 429/684 (62.72%), Query Frame = 0

Query: 113 KALAKSTLYSSPKVDSPAKITTVTS---PKEALKSTPITPKTPLIGTTGNEEEDDEEVYK 172
           K   +S L   P    P +   + S   PK     +    K+P   T G EEEDD     
Sbjct: 170 KMRTRSRLSDPPTPQLPPQTADMKSGRIPKSGQMKSGFFGKSP--KTQGEEEEDDP---F 229

Query: 173 TAELKVKEKSGKRLKKTVLMEWVAFLCLTGCLIASLTIDKLVTKEIWGLGLWKWCVLVLV 232
            AE   +E    +L   +++EW++ + +    + +L I  L  K++W L LWKW  +VLV
Sbjct: 230 AAEDLPEEYRKDKLSLWIVLEWLSLILIIAGFVCTLAIPSLRKKKLWELQLWKWESMVLV 289

Query: 233 IFCGRLFSQWFINCLVFLIERNFLLKRKVLYFVYGLKKSVIIFIWLALVLLAWGLLFDQS 292
           + CGRL S W +  +VF IERNFLL+++VLYFVYG++K+V   +WL LVLLAW  LFD+ 
Sbjct: 290 LICGRLVSSWIVKIVVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLLAWHFLFDE- 349

Query: 293 SKRSKKGN-EILNYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQY 352
            K +K  N + L  VT+     L+G  LWLVKTLLVK+LA+SF  + +FDRIQES+F QY
Sbjct: 350 -KVAKAANTKALRVVTKIFVCLLVGFLLWLVKTLLVKVLASSFHMSTYFDRIQESLFTQY 409

Query: 353 ILRILSGPPLMEM------AERVG--------------------RAASTGQLSFRHLKKE 412
           ++  LSGPPL+E+       ER+                         TG+  F      
Sbjct: 410 VIETLSGPPLIEIQKNEEEEERISVEVKKFQNPGGVEIQSGAQKSPMKTGKSPFLSHVLS 469

Query: 413 SDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIRGSGLSTISNTIENFKEEEVEQK 472
           + GG  G+ + I +D L K+  + +SAW M+ L+N+IR   L+T+   +++   +  + K
Sbjct: 470 NGGGGGGENKGITIDSLHKLNPKNVSAWKMKRLMNIIRNGSLTTLDEQLQDPSLD--DDK 529

Query: 473 DKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFMSKEEIDNVLPLFEGGAETGKIK 532
             +I SE+EA+ AA +IF NVAKPGSK+I   D+ RF+  +E    L LFEG +ET +I 
Sbjct: 530 GNQIRSEFEAKLAARKIFHNVAKPGSKFIYANDIMRFLPDDEALKTLSLFEGASETNRIS 589

Query: 533 RKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASAVVLIVIIIEWLLLMGFLTTQVL 592
           + +LKNW+VN + ER++LA +LNDTKTA+  L+K+ + VV I+I++ WL+++G  +T+ L
Sbjct: 590 KSSLKNWVVNAFRERRALALTLNDTKTAVNRLHKMVNIVVGIIILVIWLIILGITSTKFL 649

Query: 593 VFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGDRCVVDGVQMIVEEMNILTTIFL 652
           V +SSQ+++V F+FGN  + VFE+II++FV+HPFDVGDRC +DGVQM+VEEMNILTT+FL
Sbjct: 650 VVMSSQVVVVAFIFGNMCKIVFESIIYLFVIHPFDVGDRCEIDGVQMVVEEMNILTTVFL 709

Query: 653 RYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDFSTSIESIGALKARIKTYLESKP 712
           R+DN+K+ YPNS+L TK I NYYRSP+M D I+FS+  +T  E I  +K RI +Y+E K 
Sbjct: 710 RFDNQKVVYPNSLLWTKSIGNYYRSPDMGDGIEFSIHITTPAEKIILIKQRITSYIEGKK 769

Query: 713 QFWRPNYSVVVKEIENVDKMKLSLCVNHTINFQNYGDKSNRRSDLVLELKKIFEDLGIKY 767
             W P   +V K++E+++ +++++   H +N Q+ G+K  RRS LV E+ KI  +L I+Y
Sbjct: 770 DHWYPAPMIVFKDMESLNSVRIAVWPTHRMNHQDMGEKWARRSQLVEEIAKICRELDIEY 829

BLAST of PI0002153 vs. ExPASy TrEMBL
Match: A0A1S3BR08 (Mechanosensitive ion channel protein OS=Cucumis melo OX=3656 GN=LOC103492355 PE=3 SV=1)

HSP 1 Score: 1427.2 bits (3693), Expect = 0.0e+00
Identity = 744/768 (96.88%), Postives = 755/768 (98.31%), Query Frame = 0

Query: 1   MADKKGMEQLVLRILEGEEGVHGSSKDLNKPSVGSFPDFDLKETRSFRCTIPQSVVGSSP 60
           MADKKGMEQLVLRILEGEEGVHGSSKDLNKPSVGSFPDFDLKETRSFRCTIP+S+VGSSP
Sbjct: 1   MADKKGMEQLVLRILEGEEGVHGSSKDLNKPSVGSFPDFDLKETRSFRCTIPRSLVGSSP 60

Query: 61  SHEISRMTPLKPPKIPGETVTRRASFACSSFSKPKSRLIESPCPDGASLAEEKALAKSTL 120
           SHEISRMTPLKPPKIPGETVTRRASFACSSFSKPKSRLIE PCPDG SLAEEKALAKS+L
Sbjct: 61  SHEISRMTPLKPPKIPGETVTRRASFACSSFSKPKSRLIEPPCPDGESLAEEKALAKSSL 120

Query: 121 YSSPKVDSPAKITTVTSPKEALKSTPITPKTPLIGTTGNEEEDDEEVYKTAELKVKEKSG 180
           Y SPKVDSPAKIT VTSPKEALK+TPITPKTPLIGTTGNEEEDDEEVYKTAELKVKEKS 
Sbjct: 121 YGSPKVDSPAKITAVTSPKEALKATPITPKTPLIGTTGNEEEDDEEVYKTAELKVKEKSE 180

Query: 181 KRLKKTVLMEWVAFLCLTGCLIASLTIDKLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240
           KRLKKTV++EWVAFLCLTGCLIASLTID LVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF
Sbjct: 181 KRLKKTVIVEWVAFLCLTGCLIASLTIDTLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240

Query: 241 INCLVFLIERNFLLKRKVLYFVYGLKKSVIIFIWLALVLLAWGLLFDQSSKRSKKGNEIL 300
           INCLVFLIERNFLLKRKVLYFVYGLKKSVIIFIWLALVLLAWGLLFDQSSKRSKKGNEIL
Sbjct: 241 INCLVFLIERNFLLKRKVLYFVYGLKKSVIIFIWLALVLLAWGLLFDQSSKRSKKGNEIL 300

Query: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME 360
           NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME
Sbjct: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME 360

Query: 361 MAERVGRAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420
           MA RVGRAASTGQLSF+HLK+ESD GNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR
Sbjct: 361 MAGRVGRAASTGQLSFKHLKQESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420

Query: 421 GSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM 480
           GSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM
Sbjct: 421 GSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM 480

Query: 481 SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASA 540
           SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASA
Sbjct: 481 SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASA 540

Query: 541 VVLIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600
           VVLIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD
Sbjct: 541 VVLIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600

Query: 601 RCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF 660
           RCVVDGVQM+VEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF
Sbjct: 601 RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF 660

Query: 661 STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVDKMKLSLCVNHTINFQNYGDK 720
           STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENV+KMKL+LCVNHTINFQNYGDK
Sbjct: 661 STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQNYGDK 720

Query: 721 SNRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVSSAAGVV--PPSQR 767
           SNRRSDLVLELKKIFEDLGIKYHLLPQ VQLNY SSAAG V  PPSQR
Sbjct: 721 SNRRSDLVLELKKIFEDLGIKYHLLPQAVQLNYASSAAGGVPLPPSQR 768

BLAST of PI0002153 vs. ExPASy TrEMBL
Match: A0A5D3CFQ7 (Mechanosensitive ion channel protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001770 PE=3 SV=1)

HSP 1 Score: 1427.2 bits (3693), Expect = 0.0e+00
Identity = 744/768 (96.88%), Postives = 755/768 (98.31%), Query Frame = 0

Query: 1   MADKKGMEQLVLRILEGEEGVHGSSKDLNKPSVGSFPDFDLKETRSFRCTIPQSVVGSSP 60
           MADKKGMEQLVLRILEGEEGVHGSSKDLNKPSVGSFPDFDLKETRSFRCTIP+S+VGSSP
Sbjct: 1   MADKKGMEQLVLRILEGEEGVHGSSKDLNKPSVGSFPDFDLKETRSFRCTIPRSLVGSSP 60

Query: 61  SHEISRMTPLKPPKIPGETVTRRASFACSSFSKPKSRLIESPCPDGASLAEEKALAKSTL 120
           SHEISRMTPLKPPKIPGETVTRRASFACSSFSKPKSRLIE PCPDG SLAEEKALAKS+L
Sbjct: 61  SHEISRMTPLKPPKIPGETVTRRASFACSSFSKPKSRLIEPPCPDGESLAEEKALAKSSL 120

Query: 121 YSSPKVDSPAKITTVTSPKEALKSTPITPKTPLIGTTGNEEEDDEEVYKTAELKVKEKSG 180
           Y SPKVDSPAKIT VTSPKEALK+TPITPKTPLIGTTGNEEEDDEEVYKTAELKVKEKS 
Sbjct: 121 YGSPKVDSPAKITAVTSPKEALKATPITPKTPLIGTTGNEEEDDEEVYKTAELKVKEKSE 180

Query: 181 KRLKKTVLMEWVAFLCLTGCLIASLTIDKLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240
           KRLKKTV++EWVAFLCLTGCLIASLTID LVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF
Sbjct: 181 KRLKKTVIVEWVAFLCLTGCLIASLTIDTLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240

Query: 241 INCLVFLIERNFLLKRKVLYFVYGLKKSVIIFIWLALVLLAWGLLFDQSSKRSKKGNEIL 300
           INCLVFLIERNFLLKRKVLYFVYGLKKSVIIFIWLALVLLAWGLLFDQSSKRSKKGNEIL
Sbjct: 241 INCLVFLIERNFLLKRKVLYFVYGLKKSVIIFIWLALVLLAWGLLFDQSSKRSKKGNEIL 300

Query: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME 360
           NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME
Sbjct: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME 360

Query: 361 MAERVGRAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420
           MA RVGRAASTGQLSF+HLK+ESD GNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR
Sbjct: 361 MAGRVGRAASTGQLSFKHLKQESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420

Query: 421 GSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM 480
           GSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM
Sbjct: 421 GSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM 480

Query: 481 SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASA 540
           SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASA
Sbjct: 481 SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASA 540

Query: 541 VVLIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600
           VVLIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD
Sbjct: 541 VVLIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600

Query: 601 RCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF 660
           RCVVDGVQM+VEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF
Sbjct: 601 RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF 660

Query: 661 STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVDKMKLSLCVNHTINFQNYGDK 720
           STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENV+KMKL+LCVNHTINFQNYGDK
Sbjct: 661 STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQNYGDK 720

Query: 721 SNRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVSSAAGVV--PPSQR 767
           SNRRSDLVLELKKIFEDLGIKYHLLPQ VQLNY SSAAG V  PPSQR
Sbjct: 721 SNRRSDLVLELKKIFEDLGIKYHLLPQAVQLNYASSAAGGVPLPPSQR 768

BLAST of PI0002153 vs. ExPASy TrEMBL
Match: A0A5A7UIR3 (Mechanosensitive ion channel protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G001820 PE=3 SV=1)

HSP 1 Score: 1424.8 bits (3687), Expect = 0.0e+00
Identity = 743/768 (96.74%), Postives = 755/768 (98.31%), Query Frame = 0

Query: 1   MADKKGMEQLVLRILEGEEGVHGSSKDLNKPSVGSFPDFDLKETRSFRCTIPQSVVGSSP 60
           MADKKGMEQLVLRILEG EGVHGSSKDLNKPSVGSFPDFDLKETRSFRCTIP+S+VGSSP
Sbjct: 1   MADKKGMEQLVLRILEGGEGVHGSSKDLNKPSVGSFPDFDLKETRSFRCTIPRSLVGSSP 60

Query: 61  SHEISRMTPLKPPKIPGETVTRRASFACSSFSKPKSRLIESPCPDGASLAEEKALAKSTL 120
           SHEISRMTPLKPPKIPGETVTRRASFACSSFSKPKSRLIE PCPDG SLAEEKALAKS+L
Sbjct: 61  SHEISRMTPLKPPKIPGETVTRRASFACSSFSKPKSRLIEPPCPDGESLAEEKALAKSSL 120

Query: 121 YSSPKVDSPAKITTVTSPKEALKSTPITPKTPLIGTTGNEEEDDEEVYKTAELKVKEKSG 180
           YSSPKVDSPAKIT VTSPKEALK+TPITPKTPLIGTTGNEEEDDEEVYKTAELKVKEKS 
Sbjct: 121 YSSPKVDSPAKITAVTSPKEALKATPITPKTPLIGTTGNEEEDDEEVYKTAELKVKEKSE 180

Query: 181 KRLKKTVLMEWVAFLCLTGCLIASLTIDKLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240
           KRLKKTV++EWVAFLCLTGCLIASLTID LVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF
Sbjct: 181 KRLKKTVIVEWVAFLCLTGCLIASLTIDTLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240

Query: 241 INCLVFLIERNFLLKRKVLYFVYGLKKSVIIFIWLALVLLAWGLLFDQSSKRSKKGNEIL 300
           INCLVFLIERNFLLKRKVLYFVYGLKKSVIIFIWLALVLLAWGLLFDQSSKRSKKGNEIL
Sbjct: 241 INCLVFLIERNFLLKRKVLYFVYGLKKSVIIFIWLALVLLAWGLLFDQSSKRSKKGNEIL 300

Query: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME 360
           NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME
Sbjct: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME 360

Query: 361 MAERVGRAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420
           MA RVGRAASTGQLSF+HLK+ESD GNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR
Sbjct: 361 MAGRVGRAASTGQLSFKHLKQESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420

Query: 421 GSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM 480
           GSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPG+KYIDEEDLFRFM
Sbjct: 421 GSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGNKYIDEEDLFRFM 480

Query: 481 SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASA 540
           SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASA
Sbjct: 481 SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASA 540

Query: 541 VVLIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600
           VVLIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD
Sbjct: 541 VVLIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600

Query: 601 RCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF 660
           RCVVDGVQM+VEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF
Sbjct: 601 RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF 660

Query: 661 STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVDKMKLSLCVNHTINFQNYGDK 720
           STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENV+KMKL+LCVNHTINFQNYGDK
Sbjct: 661 STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQNYGDK 720

Query: 721 SNRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVSSAAGVV--PPSQR 767
           SNRRSDLVLELKKIFEDLGIKYHLLPQ VQLNY SSAAG V  PPSQR
Sbjct: 721 SNRRSDLVLELKKIFEDLGIKYHLLPQAVQLNYASSAAGGVPLPPSQR 768

BLAST of PI0002153 vs. ExPASy TrEMBL
Match: A0A0A0LYZ9 (Mechanosensitive ion channel protein OS=Cucumis sativus OX=3659 GN=Csa_1G595830 PE=3 SV=1)

HSP 1 Score: 1412.9 bits (3656), Expect = 0.0e+00
Identity = 735/766 (95.95%), Postives = 753/766 (98.30%), Query Frame = 0

Query: 1   MADKKGMEQLVLRILEGEEGVHGSSKDLNKPSVGSFPDFDLKETRSFRCTIPQSVVGSSP 60
           MADKKGMEQLVLRILEGEEGV  SSKDLNKPSVGSFPDFDLKETRSFRCTIPQSVVGSSP
Sbjct: 1   MADKKGMEQLVLRILEGEEGVRESSKDLNKPSVGSFPDFDLKETRSFRCTIPQSVVGSSP 60

Query: 61  SHEISRMTPLKPPKIPGETVTRRASFACSSFSKPKSRLIESPCPDGASLAEEKALAKSTL 120
           SHEISRMTP KPPKIPGETVTRRASFACSSF+KPKSRLIE PCPDGASLAEEK LAKSTL
Sbjct: 61  SHEISRMTPHKPPKIPGETVTRRASFACSSFTKPKSRLIEPPCPDGASLAEEKELAKSTL 120

Query: 121 YSSPKVDSPAKITTVTSPKEALKSTPITPKTPLIGTTGNEEEDDEEVYKTAELKVKEKSG 180
           YSS KVDSPAKITTVTSPKEALK+ PITPKTPLIGTTGNEEEDDEEVY+TAELKVKEKSG
Sbjct: 121 YSSSKVDSPAKITTVTSPKEALKAAPITPKTPLIGTTGNEEEDDEEVYRTAELKVKEKSG 180

Query: 181 KRLKKTVLMEWVAFLCLTGCLIASLTIDKLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240
           KRLKKTV++EW+AFLCLTGCLIASLTI+ LVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF
Sbjct: 181 KRLKKTVIVEWIAFLCLTGCLIASLTIETLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240

Query: 241 INCLVFLIERNFLLKRKVLYFVYGLKKSVIIFIWLALVLLAWGLLFDQSSKRSKKGNEIL 300
           INCLVFLIERNFLLKRKVLYFVYGL+KSVIIFIWLALVLLAWGLLFDQSSKRSK+GN+IL
Sbjct: 241 INCLVFLIERNFLLKRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDQSSKRSKEGNKIL 300

Query: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME 360
           NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME
Sbjct: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME 360

Query: 361 MAERVGRAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420
           MAERVGRAASTGQLSF+HLKKESD GNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR
Sbjct: 361 MAERVGRAASTGQLSFKHLKKESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420

Query: 421 GSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM 480
           GSGLSTISNTIENFKEEEVE+KDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM
Sbjct: 421 GSGLSTISNTIENFKEEEVEKKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM 480

Query: 481 SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASA 540
           SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKL+SA
Sbjct: 481 SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLSSA 540

Query: 541 VVLIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600
           V+LIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD
Sbjct: 541 VILIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600

Query: 601 RCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF 660
           RCVVDGVQM+VEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF
Sbjct: 601 RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF 660

Query: 661 STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVDKMKLSLCVNHTINFQNYGDK 720
           STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENV+KMKL+LCVNHTINFQNYGDK
Sbjct: 661 STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQNYGDK 720

Query: 721 SNRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVSSAAGVVPPSQR 767
           SNRRSDLVLELKKIFE+LGIKYHLLPQEVQLNYVSSAA    PSQR
Sbjct: 721 SNRRSDLVLELKKIFEELGIKYHLLPQEVQLNYVSSAA----PSQR 762

BLAST of PI0002153 vs. ExPASy TrEMBL
Match: A0A6J1JDR1 (Mechanosensitive ion channel protein OS=Cucurbita maxima OX=3661 GN=LOC111484882 PE=3 SV=1)

HSP 1 Score: 1293.1 bits (3345), Expect = 0.0e+00
Identity = 677/770 (87.92%), Postives = 715/770 (92.86%), Query Frame = 0

Query: 1   MADKKGMEQLVLRILEGEEGVHGSSKDLNKPSVGSFPDFDLKETRSFRCTIPQSVVGSSP 60
           MADKKG EQ+VLRIL+GEEGV  +   L   SV SFPDF+ KETRS RC IPQSVV SSP
Sbjct: 1   MADKKGTEQVVLRILDGEEGV-DARNGLTNGSVASFPDFESKETRSVRCAIPQSVVESSP 60

Query: 61  SHEISRMTPLKPPKIPGETVTRRASFACSSFSKPKSRLIESPCPDGASLAEEKALAKST- 120
           SHEISRM+ LKPPKIP E+  RR SFA SSFSKPKSRLIE PCPDGAS AEE A AKST 
Sbjct: 61  SHEISRMSSLKPPKIPVESAVRRPSFARSSFSKPKSRLIEPPCPDGASSAEENAQAKSTS 120

Query: 121 ---LYSSPKVDSPAKITTVTSPKEALKSTPITPKTPLIGTTGNEEEDDEEVYKTAELKVK 180
                SSPK+DSPAKI T TSPKE+LKS PITP+TPL+G+TG+EEEDDEEVYKTAELKVK
Sbjct: 121 GSPFSSSPKMDSPAKIATATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVK 180

Query: 181 EKSGKRLKKTVLMEWVAFLCLTGCLIASLTIDKLVTKEIWGLGLWKWCVLVLVIFCGRLF 240
           EKS K+LK+ VL+EW+AFLC+T CLI+SLTIDKL+TKEIWGLGLWKWCVLVLVIFCGRLF
Sbjct: 181 EKSRKKLKRAVLIEWIAFLCITACLISSLTIDKLLTKEIWGLGLWKWCVLVLVIFCGRLF 240

Query: 241 SQWFINCLVFLIERNFLLKRKVLYFVYGLKKSVIIFIWLALVLLAWGLLFDQSSKRSKKG 300
           SQWFINCLVFLIERNFLLKRKVLYFVYGL+KSVI+FIWLALVLLAWGLLFDQS KRSKK 
Sbjct: 241 SQWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLALVLLAWGLLFDQSIKRSKKV 300

Query: 301 NEILNYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGP 360
           NEILNYVTRAL ASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGP
Sbjct: 301 NEILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGP 360

Query: 361 PLMEMAERVGRAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLI 420
           PLMEMAERVGR AS GQLSFRHLKKE DGGNEGKEEVIDVDKLKKMKQ K+SAWTMRGLI
Sbjct: 361 PLMEMAERVGRVASAGQLSFRHLKKERDGGNEGKEEVIDVDKLKKMKQAKVSAWTMRGLI 420

Query: 421 NVIRGSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDL 480
           NVIR SGLSTISNTIENFKEEE EQKDKEINSEWEA AAAYQIFRNVAKPGSKYIDEEDL
Sbjct: 421 NVIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDL 480

Query: 481 FRFMSKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNK 540
           FRFM+KEEIDNVLPLFEGG ETGKIKRKTLKNWLVNVY+ERKSLAHSLNDTKTAIEELN+
Sbjct: 481 FRFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNR 540

Query: 541 LASAVVLIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPF 600
           LASA VLIVIII WLLLMGFLTTQVLVFISSQ+LLVVFMFGNTA+T+FEAIIFVFVMHPF
Sbjct: 541 LASAFVLIVIIIVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPF 600

Query: 601 DVGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDF 660
           DVGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISN+YRSPEMSDSIDF
Sbjct: 601 DVGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIDF 660

Query: 661 SVDFSTSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVDKMKLSLCVNHTINFQN 720
           SVDFSTSIESIGALKARIK+YLESKPQFWRPN+SV+VKEIENV+KMKLSL +NHTINFQN
Sbjct: 661 SVDFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQN 720

Query: 721 YGDKSNRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVSSAAGVVPPSQR 767
           YGD+S+RRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVSS A +V  + R
Sbjct: 721 YGDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVSSIAPMVSTTSR 769

BLAST of PI0002153 vs. NCBI nr
Match: XP_008450905.1 (PREDICTED: mechanosensitive ion channel protein 10-like [Cucumis melo] >XP_008450906.1 PREDICTED: mechanosensitive ion channel protein 10-like [Cucumis melo] >TYK10044.1 mechanosensitive ion channel protein 10-like [Cucumis melo var. makuwa])

HSP 1 Score: 1427.2 bits (3693), Expect = 0.0e+00
Identity = 744/768 (96.88%), Postives = 755/768 (98.31%), Query Frame = 0

Query: 1   MADKKGMEQLVLRILEGEEGVHGSSKDLNKPSVGSFPDFDLKETRSFRCTIPQSVVGSSP 60
           MADKKGMEQLVLRILEGEEGVHGSSKDLNKPSVGSFPDFDLKETRSFRCTIP+S+VGSSP
Sbjct: 1   MADKKGMEQLVLRILEGEEGVHGSSKDLNKPSVGSFPDFDLKETRSFRCTIPRSLVGSSP 60

Query: 61  SHEISRMTPLKPPKIPGETVTRRASFACSSFSKPKSRLIESPCPDGASLAEEKALAKSTL 120
           SHEISRMTPLKPPKIPGETVTRRASFACSSFSKPKSRLIE PCPDG SLAEEKALAKS+L
Sbjct: 61  SHEISRMTPLKPPKIPGETVTRRASFACSSFSKPKSRLIEPPCPDGESLAEEKALAKSSL 120

Query: 121 YSSPKVDSPAKITTVTSPKEALKSTPITPKTPLIGTTGNEEEDDEEVYKTAELKVKEKSG 180
           Y SPKVDSPAKIT VTSPKEALK+TPITPKTPLIGTTGNEEEDDEEVYKTAELKVKEKS 
Sbjct: 121 YGSPKVDSPAKITAVTSPKEALKATPITPKTPLIGTTGNEEEDDEEVYKTAELKVKEKSE 180

Query: 181 KRLKKTVLMEWVAFLCLTGCLIASLTIDKLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240
           KRLKKTV++EWVAFLCLTGCLIASLTID LVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF
Sbjct: 181 KRLKKTVIVEWVAFLCLTGCLIASLTIDTLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240

Query: 241 INCLVFLIERNFLLKRKVLYFVYGLKKSVIIFIWLALVLLAWGLLFDQSSKRSKKGNEIL 300
           INCLVFLIERNFLLKRKVLYFVYGLKKSVIIFIWLALVLLAWGLLFDQSSKRSKKGNEIL
Sbjct: 241 INCLVFLIERNFLLKRKVLYFVYGLKKSVIIFIWLALVLLAWGLLFDQSSKRSKKGNEIL 300

Query: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME 360
           NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME
Sbjct: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME 360

Query: 361 MAERVGRAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420
           MA RVGRAASTGQLSF+HLK+ESD GNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR
Sbjct: 361 MAGRVGRAASTGQLSFKHLKQESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420

Query: 421 GSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM 480
           GSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM
Sbjct: 421 GSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM 480

Query: 481 SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASA 540
           SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASA
Sbjct: 481 SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASA 540

Query: 541 VVLIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600
           VVLIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD
Sbjct: 541 VVLIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600

Query: 601 RCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF 660
           RCVVDGVQM+VEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF
Sbjct: 601 RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF 660

Query: 661 STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVDKMKLSLCVNHTINFQNYGDK 720
           STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENV+KMKL+LCVNHTINFQNYGDK
Sbjct: 661 STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQNYGDK 720

Query: 721 SNRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVSSAAGVV--PPSQR 767
           SNRRSDLVLELKKIFEDLGIKYHLLPQ VQLNY SSAAG V  PPSQR
Sbjct: 721 SNRRSDLVLELKKIFEDLGIKYHLLPQAVQLNYASSAAGGVPLPPSQR 768

BLAST of PI0002153 vs. NCBI nr
Match: KAA0055793.1 (mechanosensitive ion channel protein 10-like [Cucumis melo var. makuwa])

HSP 1 Score: 1424.8 bits (3687), Expect = 0.0e+00
Identity = 743/768 (96.74%), Postives = 755/768 (98.31%), Query Frame = 0

Query: 1   MADKKGMEQLVLRILEGEEGVHGSSKDLNKPSVGSFPDFDLKETRSFRCTIPQSVVGSSP 60
           MADKKGMEQLVLRILEG EGVHGSSKDLNKPSVGSFPDFDLKETRSFRCTIP+S+VGSSP
Sbjct: 1   MADKKGMEQLVLRILEGGEGVHGSSKDLNKPSVGSFPDFDLKETRSFRCTIPRSLVGSSP 60

Query: 61  SHEISRMTPLKPPKIPGETVTRRASFACSSFSKPKSRLIESPCPDGASLAEEKALAKSTL 120
           SHEISRMTPLKPPKIPGETVTRRASFACSSFSKPKSRLIE PCPDG SLAEEKALAKS+L
Sbjct: 61  SHEISRMTPLKPPKIPGETVTRRASFACSSFSKPKSRLIEPPCPDGESLAEEKALAKSSL 120

Query: 121 YSSPKVDSPAKITTVTSPKEALKSTPITPKTPLIGTTGNEEEDDEEVYKTAELKVKEKSG 180
           YSSPKVDSPAKIT VTSPKEALK+TPITPKTPLIGTTGNEEEDDEEVYKTAELKVKEKS 
Sbjct: 121 YSSPKVDSPAKITAVTSPKEALKATPITPKTPLIGTTGNEEEDDEEVYKTAELKVKEKSE 180

Query: 181 KRLKKTVLMEWVAFLCLTGCLIASLTIDKLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240
           KRLKKTV++EWVAFLCLTGCLIASLTID LVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF
Sbjct: 181 KRLKKTVIVEWVAFLCLTGCLIASLTIDTLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240

Query: 241 INCLVFLIERNFLLKRKVLYFVYGLKKSVIIFIWLALVLLAWGLLFDQSSKRSKKGNEIL 300
           INCLVFLIERNFLLKRKVLYFVYGLKKSVIIFIWLALVLLAWGLLFDQSSKRSKKGNEIL
Sbjct: 241 INCLVFLIERNFLLKRKVLYFVYGLKKSVIIFIWLALVLLAWGLLFDQSSKRSKKGNEIL 300

Query: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME 360
           NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME
Sbjct: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME 360

Query: 361 MAERVGRAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420
           MA RVGRAASTGQLSF+HLK+ESD GNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR
Sbjct: 361 MAGRVGRAASTGQLSFKHLKQESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420

Query: 421 GSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM 480
           GSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPG+KYIDEEDLFRFM
Sbjct: 421 GSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGNKYIDEEDLFRFM 480

Query: 481 SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASA 540
           SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASA
Sbjct: 481 SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASA 540

Query: 541 VVLIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600
           VVLIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD
Sbjct: 541 VVLIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600

Query: 601 RCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF 660
           RCVVDGVQM+VEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF
Sbjct: 601 RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF 660

Query: 661 STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVDKMKLSLCVNHTINFQNYGDK 720
           STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENV+KMKL+LCVNHTINFQNYGDK
Sbjct: 661 STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQNYGDK 720

Query: 721 SNRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVSSAAGVV--PPSQR 767
           SNRRSDLVLELKKIFEDLGIKYHLLPQ VQLNY SSAAG V  PPSQR
Sbjct: 721 SNRRSDLVLELKKIFEDLGIKYHLLPQAVQLNYASSAAGGVPLPPSQR 768

BLAST of PI0002153 vs. NCBI nr
Match: XP_031738755.1 (mechanosensitive ion channel protein 10 [Cucumis sativus] >KGN66279.1 hypothetical protein Csa_007026 [Cucumis sativus])

HSP 1 Score: 1412.9 bits (3656), Expect = 0.0e+00
Identity = 735/766 (95.95%), Postives = 753/766 (98.30%), Query Frame = 0

Query: 1   MADKKGMEQLVLRILEGEEGVHGSSKDLNKPSVGSFPDFDLKETRSFRCTIPQSVVGSSP 60
           MADKKGMEQLVLRILEGEEGV  SSKDLNKPSVGSFPDFDLKETRSFRCTIPQSVVGSSP
Sbjct: 1   MADKKGMEQLVLRILEGEEGVRESSKDLNKPSVGSFPDFDLKETRSFRCTIPQSVVGSSP 60

Query: 61  SHEISRMTPLKPPKIPGETVTRRASFACSSFSKPKSRLIESPCPDGASLAEEKALAKSTL 120
           SHEISRMTP KPPKIPGETVTRRASFACSSF+KPKSRLIE PCPDGASLAEEK LAKSTL
Sbjct: 61  SHEISRMTPHKPPKIPGETVTRRASFACSSFTKPKSRLIEPPCPDGASLAEEKELAKSTL 120

Query: 121 YSSPKVDSPAKITTVTSPKEALKSTPITPKTPLIGTTGNEEEDDEEVYKTAELKVKEKSG 180
           YSS KVDSPAKITTVTSPKEALK+ PITPKTPLIGTTGNEEEDDEEVY+TAELKVKEKSG
Sbjct: 121 YSSSKVDSPAKITTVTSPKEALKAAPITPKTPLIGTTGNEEEDDEEVYRTAELKVKEKSG 180

Query: 181 KRLKKTVLMEWVAFLCLTGCLIASLTIDKLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240
           KRLKKTV++EW+AFLCLTGCLIASLTI+ LVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF
Sbjct: 181 KRLKKTVIVEWIAFLCLTGCLIASLTIETLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240

Query: 241 INCLVFLIERNFLLKRKVLYFVYGLKKSVIIFIWLALVLLAWGLLFDQSSKRSKKGNEIL 300
           INCLVFLIERNFLLKRKVLYFVYGL+KSVIIFIWLALVLLAWGLLFDQSSKRSK+GN+IL
Sbjct: 241 INCLVFLIERNFLLKRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDQSSKRSKEGNKIL 300

Query: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME 360
           NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME
Sbjct: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME 360

Query: 361 MAERVGRAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420
           MAERVGRAASTGQLSF+HLKKESD GNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR
Sbjct: 361 MAERVGRAASTGQLSFKHLKKESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420

Query: 421 GSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM 480
           GSGLSTISNTIENFKEEEVE+KDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM
Sbjct: 421 GSGLSTISNTIENFKEEEVEKKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM 480

Query: 481 SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASA 540
           SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKL+SA
Sbjct: 481 SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLSSA 540

Query: 541 VVLIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600
           V+LIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD
Sbjct: 541 VILIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600

Query: 601 RCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF 660
           RCVVDGVQM+VEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF
Sbjct: 601 RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF 660

Query: 661 STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVDKMKLSLCVNHTINFQNYGDK 720
           STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENV+KMKL+LCVNHTINFQNYGDK
Sbjct: 661 STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQNYGDK 720

Query: 721 SNRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVSSAAGVVPPSQR 767
           SNRRSDLVLELKKIFE+LGIKYHLLPQEVQLNYVSSAA    PSQR
Sbjct: 721 SNRRSDLVLELKKIFEELGIKYHLLPQEVQLNYVSSAA----PSQR 762

BLAST of PI0002153 vs. NCBI nr
Match: XP_038878911.1 (mechanosensitive ion channel protein 10-like [Benincasa hispida])

HSP 1 Score: 1390.6 bits (3598), Expect = 0.0e+00
Identity = 720/766 (93.99%), Postives = 745/766 (97.26%), Query Frame = 0

Query: 1   MADKKGMEQLVLRILEGEEGVHGSSKDLNKPSVGSFPDFDLKETRSFRCTIPQSVVGSSP 60
           MADKKGMEQLVLRILEGEEGV  +SKDL KPSV SFPDFDLKETRSFRCTIPQSVVGSSP
Sbjct: 1   MADKKGMEQLVLRILEGEEGV-DTSKDLTKPSVSSFPDFDLKETRSFRCTIPQSVVGSSP 60

Query: 61  SHEISRMTPLKPPKIPGETVTRRASFACSSFSKPKSRLIESPCPDGASLAEEKALAKSTL 120
           SHEISRM+PLKPPKIPGET  RR SFA SSFSKPKSRLIESPCPD ASLAEEKA AKSTL
Sbjct: 61  SHEISRMSPLKPPKIPGETAIRRPSFARSSFSKPKSRLIESPCPDSASLAEEKAKAKSTL 120

Query: 121 YSSPKVDSPAKITTVTSPKEALKSTPITPKTPLIGTTGNEEEDDEEVYKTAELKVKEKSG 180
           YSSPK+DSPAKITTVTSPKEALKS PITPKTPLIGTTG+EEEDDEEVYKTAELKVKE+SG
Sbjct: 121 YSSPKMDSPAKITTVTSPKEALKSAPITPKTPLIGTTGSEEEDDEEVYKTAELKVKERSG 180

Query: 181 KRLKKTVLMEWVAFLCLTGCLIASLTIDKLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240
           K+LK+TVL+EWVAFLCLTGCLIASLTIDKLVTKEIWGLGLWKWCVLVLV FCGRLFSQWF
Sbjct: 181 KKLKRTVLIEWVAFLCLTGCLIASLTIDKLVTKEIWGLGLWKWCVLVLVSFCGRLFSQWF 240

Query: 241 INCLVFLIERNFLLKRKVLYFVYGLKKSVIIFIWLALVLLAWGLLFDQSSKRSKKGNEIL 300
           INCLVFLIERNFLLKRKVLYFVYGL+KSVIIFIWL LVLLAWGLLFDQSSKRSKKGNEIL
Sbjct: 241 INCLVFLIERNFLLKRKVLYFVYGLRKSVIIFIWLGLVLLAWGLLFDQSSKRSKKGNEIL 300

Query: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME 360
           NY+TRALGASLIGAGLWLVKTL+VKILAASFQC RFFDRIQESIFHQYILRILSGPP+ME
Sbjct: 301 NYITRALGASLIGAGLWLVKTLMVKILAASFQCARFFDRIQESIFHQYILRILSGPPIME 360

Query: 361 MAERVGRAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420
           MAE VGRAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR
Sbjct: 361 MAESVGRAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420

Query: 421 GSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFM 480
            SGLSTISNTIENFKEEE EQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDE+DLFRFM
Sbjct: 421 SSGLSTISNTIENFKEEENEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEDDLFRFM 480

Query: 481 SKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASA 540
           SKEEIDNVLPLFEGG ETGKIKRKTLKNWLVNVY+ERKSLAHSLNDTKTAIEELNKLASA
Sbjct: 481 SKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNKLASA 540

Query: 541 VVLIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600
           +VLIVIIIEWLLLMGFLTTQVLVF+SSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD
Sbjct: 541 IVLIVIIIEWLLLMGFLTTQVLVFVSSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600

Query: 601 RCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVDF 660
           RCVVDGVQM+VEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSI+FSVDF
Sbjct: 601 RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIEFSVDF 660

Query: 661 STSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVDKMKLSLCVNHTINFQNYGDK 720
           STSIESIGALKARIKTYLESKPQFWRPN+SV+VKEIEN++KMKL+LCVNHTINFQNYGDK
Sbjct: 661 STSIESIGALKARIKTYLESKPQFWRPNHSVIVKEIENINKMKLALCVNHTINFQNYGDK 720

Query: 721 SNRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVSSAAGVVPPSQR 767
           S+RRSDLVLELKKIFEDLGIKYHLLPQEVQLNYV SAA V+P SQR
Sbjct: 721 SSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVGSAASVIPSSQR 765

BLAST of PI0002153 vs. NCBI nr
Match: XP_022987285.1 (mechanosensitive ion channel protein 10-like [Cucurbita maxima] >XP_022987286.1 mechanosensitive ion channel protein 10-like [Cucurbita maxima])

HSP 1 Score: 1293.1 bits (3345), Expect = 0.0e+00
Identity = 677/770 (87.92%), Postives = 715/770 (92.86%), Query Frame = 0

Query: 1   MADKKGMEQLVLRILEGEEGVHGSSKDLNKPSVGSFPDFDLKETRSFRCTIPQSVVGSSP 60
           MADKKG EQ+VLRIL+GEEGV  +   L   SV SFPDF+ KETRS RC IPQSVV SSP
Sbjct: 1   MADKKGTEQVVLRILDGEEGV-DARNGLTNGSVASFPDFESKETRSVRCAIPQSVVESSP 60

Query: 61  SHEISRMTPLKPPKIPGETVTRRASFACSSFSKPKSRLIESPCPDGASLAEEKALAKST- 120
           SHEISRM+ LKPPKIP E+  RR SFA SSFSKPKSRLIE PCPDGAS AEE A AKST 
Sbjct: 61  SHEISRMSSLKPPKIPVESAVRRPSFARSSFSKPKSRLIEPPCPDGASSAEENAQAKSTS 120

Query: 121 ---LYSSPKVDSPAKITTVTSPKEALKSTPITPKTPLIGTTGNEEEDDEEVYKTAELKVK 180
                SSPK+DSPAKI T TSPKE+LKS PITP+TPL+G+TG+EEEDDEEVYKTAELKVK
Sbjct: 121 GSPFSSSPKMDSPAKIATATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVK 180

Query: 181 EKSGKRLKKTVLMEWVAFLCLTGCLIASLTIDKLVTKEIWGLGLWKWCVLVLVIFCGRLF 240
           EKS K+LK+ VL+EW+AFLC+T CLI+SLTIDKL+TKEIWGLGLWKWCVLVLVIFCGRLF
Sbjct: 181 EKSRKKLKRAVLIEWIAFLCITACLISSLTIDKLLTKEIWGLGLWKWCVLVLVIFCGRLF 240

Query: 241 SQWFINCLVFLIERNFLLKRKVLYFVYGLKKSVIIFIWLALVLLAWGLLFDQSSKRSKKG 300
           SQWFINCLVFLIERNFLLKRKVLYFVYGL+KSVI+FIWLALVLLAWGLLFDQS KRSKK 
Sbjct: 241 SQWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLALVLLAWGLLFDQSIKRSKKV 300

Query: 301 NEILNYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGP 360
           NEILNYVTRAL ASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGP
Sbjct: 301 NEILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGP 360

Query: 361 PLMEMAERVGRAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLI 420
           PLMEMAERVGR AS GQLSFRHLKKE DGGNEGKEEVIDVDKLKKMKQ K+SAWTMRGLI
Sbjct: 361 PLMEMAERVGRVASAGQLSFRHLKKERDGGNEGKEEVIDVDKLKKMKQAKVSAWTMRGLI 420

Query: 421 NVIRGSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDL 480
           NVIR SGLSTISNTIENFKEEE EQKDKEINSEWEA AAAYQIFRNVAKPGSKYIDEEDL
Sbjct: 421 NVIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDL 480

Query: 481 FRFMSKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNK 540
           FRFM+KEEIDNVLPLFEGG ETGKIKRKTLKNWLVNVY+ERKSLAHSLNDTKTAIEELN+
Sbjct: 481 FRFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNR 540

Query: 541 LASAVVLIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPF 600
           LASA VLIVIII WLLLMGFLTTQVLVFISSQ+LLVVFMFGNTA+T+FEAIIFVFVMHPF
Sbjct: 541 LASAFVLIVIIIVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPF 600

Query: 601 DVGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDF 660
           DVGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISN+YRSPEMSDSIDF
Sbjct: 601 DVGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRSPEMSDSIDF 660

Query: 661 SVDFSTSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVDKMKLSLCVNHTINFQN 720
           SVDFSTSIESIGALKARIK+YLESKPQFWRPN+SV+VKEIENV+KMKLSL +NHTINFQN
Sbjct: 661 SVDFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQN 720

Query: 721 YGDKSNRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVSSAAGVVPPSQR 767
           YGD+S+RRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVSS A +V  + R
Sbjct: 721 YGDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVSSIAPMVSTTSR 769

BLAST of PI0002153 vs. TAIR 10
Match: AT5G12080.1 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 675.2 bits (1741), Expect = 6.0e-194
Identity = 371/717 (51.74%), Postives = 504/717 (70.29%), Query Frame = 0

Query: 45  RSFRCTIPQSVVG----SSPSHEISRM--TPLKPPKIPGET---VTRRASFACSSFSKPK 104
           RS     P+S  G     SPS EIS++  +P KPP+ P +    +T+R SFA S +SKPK
Sbjct: 28  RSKEMASPESEKGVPFSKSPSPEISKLVGSPNKPPRAPNQNNVGLTQRKSFARSVYSKPK 87

Query: 105 SRLIESPCPDGASLAEEKALAKSTLYSSPKVDSPAKITTVTSPKEALKSTPITPKTPLIG 164
           SR ++  CP   S+ EE+   +     S    SP       S +      P+TP   ++ 
Sbjct: 88  SRFVDPSCPVDTSILEEEVREQLGAGFSFSRASPNN----KSNRSVGSPAPVTPSKVVV- 147

Query: 165 TTGNEEEDDEEVYKTAELKVKEKSGKRLKKTVLMEWVAFLCLTGCLIASLTIDKLVTKEI 224
               E+++DEE+YK  ++K+  +   ++    L+E   F+ +   L+ASLTI+ L     
Sbjct: 148 ----EKDEDEEIYK--KVKLNREMRSKISTLALIESAFFVVILSALVASLTINVLKHHTF 207

Query: 225 WGLGLWKWCVLVLVIFCGRLFSQWFINCLVFLIERNFLLKRKVLYFVYGLKKSVIIFIWL 284
           WGL +WKWCVLV+VIF G L + WF+  +VFLIE NFLL+RKVLYFV+GLKKSV +FIWL
Sbjct: 208 WGLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLIETNFLLRRKVLYFVHGLKKSVQVFIWL 267

Query: 285 ALVLLAWGLLFDQSSKRSKKGNEILNYVTRALGASLIGAGLWLVKTLLVKILAASFQCTR 344
            L+L+AW LLF+   KRS    ++L  +TR L + L GA  WLVKTLL+KILAA+F    
Sbjct: 268 CLILVAWILLFNHDVKRSPAATKVLKCITRTLISILTGAFFWLVKTLLLKILAANFNVNN 327

Query: 345 FFDRIQESIFHQYILRILSGPPLMEMAERVGRAASTGQLSFRHLKKESDGGNEGKEEVID 404
           FFDRIQ+S+FHQY+L+ LSG PLME AERVGR  STG LSF  + K+   G   +++VID
Sbjct: 328 FFDRIQDSVFHQYVLQTLSGLPLMEEAERVGREPSTGHLSFATVVKK---GTVKEKKVID 387

Query: 405 VDKLKKMKQEKISAWTMRGLINVIRGSGLSTISNTI-ENFKEEEVEQKDKEINSEWEARA 464
           + K+ KMK+EK+SAWTMR L+  +R SGLSTIS+T+ E    E  EQ D+EI SE EA A
Sbjct: 388 MGKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDTLDETAYGEGKEQADREITSEMEALA 447

Query: 465 AAYQIFRNVAKPGSKYIDEEDLFRFMSKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVY 524
           AAY +FRNVA+P   YI+EEDL RFM KEE+D V PLF+G AETG+I RK    W+V VY
Sbjct: 448 AAYHVFRNVAQPFFNYIEEEDLLRFMIKEEVDLVFPLFDGAAETGRITRKAFTEWVVKVY 507

Query: 525 VERKSLAHSLNDTKTAIEELNKLASAVVLIVIIIEWLLLMGFLTTQVLVFISSQILLVVF 584
             R++LAHSLNDTKTA+++LNKL +A++++V ++ WLLL+   TT+VL+F S+Q++ + F
Sbjct: 508 TSRRALAHSLNDTKTAVKQLNKLVTAILMVVTVVIWLLLLEVATTKVLLFFSTQLVALAF 567

Query: 585 MFGNTARTVFEAIIFVFVMHPFDVGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNS 644
           + G+T + +FE+I+FVFVMHP+DVGDRCVVDGV M+VEEMN+LTT+FL+ +NEK++YPN+
Sbjct: 568 IIGSTCKNLFESIVFVFVMHPYDVGDRCVVDGVAMLVEEMNLLTTVFLKLNNEKVYYPNA 627

Query: 645 VLATKPISNYYRSPEMSDSIDFSVDFSTSIESIGALKARIKTYLESKPQFWRPNYSVVVK 704
           VLATKPISNY+RSP M ++++FS+ FST +  I  LK RI  YLE  PQ W P +SVVVK
Sbjct: 628 VLATKPISNYFRSPNMGETVEFSISFSTPVSKIAHLKERIAEYLEQNPQHWAPVHSVVVK 687

Query: 705 EIENVDKMKLSLCVNHTINFQNYGDKSNRRSDLVLELKKIFEDLGIKYHLLPQEVQL 752
           EIEN++K+K++L  +HTI FQ   +++ RR++L L +K++ EDL I Y LLPQ++ L
Sbjct: 688 EIENMNKLKMALYSDHTITFQENRERNLRRTELSLAIKRMLEDLHIDYTLLPQDINL 730

BLAST of PI0002153 vs. TAIR 10
Match: AT5G12080.2 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 675.2 bits (1741), Expect = 6.0e-194
Identity = 371/717 (51.74%), Postives = 504/717 (70.29%), Query Frame = 0

Query: 45  RSFRCTIPQSVVG----SSPSHEISRM--TPLKPPKIPGET---VTRRASFACSSFSKPK 104
           RS     P+S  G     SPS EIS++  +P KPP+ P +    +T+R SFA S +SKPK
Sbjct: 28  RSKEMASPESEKGVPFSKSPSPEISKLVGSPNKPPRAPNQNNVGLTQRKSFARSVYSKPK 87

Query: 105 SRLIESPCPDGASLAEEKALAKSTLYSSPKVDSPAKITTVTSPKEALKSTPITPKTPLIG 164
           SR ++  CP   S+ EE+   +     S    SP       S +      P+TP   ++ 
Sbjct: 88  SRFVDPSCPVDTSILEEEVREQLGAGFSFSRASPNN----KSNRSVGSPAPVTPSKVVV- 147

Query: 165 TTGNEEEDDEEVYKTAELKVKEKSGKRLKKTVLMEWVAFLCLTGCLIASLTIDKLVTKEI 224
               E+++DEE+YK  ++K+  +   ++    L+E   F+ +   L+ASLTI+ L     
Sbjct: 148 ----EKDEDEEIYK--KVKLNREMRSKISTLALIESAFFVVILSALVASLTINVLKHHTF 207

Query: 225 WGLGLWKWCVLVLVIFCGRLFSQWFINCLVFLIERNFLLKRKVLYFVYGLKKSVIIFIWL 284
           WGL +WKWCVLV+VIF G L + WF+  +VFLIE NFLL+RKVLYFV+GLKKSV +FIWL
Sbjct: 208 WGLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLIETNFLLRRKVLYFVHGLKKSVQVFIWL 267

Query: 285 ALVLLAWGLLFDQSSKRSKKGNEILNYVTRALGASLIGAGLWLVKTLLVKILAASFQCTR 344
            L+L+AW LLF+   KRS    ++L  +TR L + L GA  WLVKTLL+KILAA+F    
Sbjct: 268 CLILVAWILLFNHDVKRSPAATKVLKCITRTLISILTGAFFWLVKTLLLKILAANFNVNN 327

Query: 345 FFDRIQESIFHQYILRILSGPPLMEMAERVGRAASTGQLSFRHLKKESDGGNEGKEEVID 404
           FFDRIQ+S+FHQY+L+ LSG PLME AERVGR  STG LSF  + K+   G   +++VID
Sbjct: 328 FFDRIQDSVFHQYVLQTLSGLPLMEEAERVGREPSTGHLSFATVVKK---GTVKEKKVID 387

Query: 405 VDKLKKMKQEKISAWTMRGLINVIRGSGLSTISNTI-ENFKEEEVEQKDKEINSEWEARA 464
           + K+ KMK+EK+SAWTMR L+  +R SGLSTIS+T+ E    E  EQ D+EI SE EA A
Sbjct: 388 MGKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDTLDETAYGEGKEQADREITSEMEALA 447

Query: 465 AAYQIFRNVAKPGSKYIDEEDLFRFMSKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVY 524
           AAY +FRNVA+P   YI+EEDL RFM KEE+D V PLF+G AETG+I RK    W+V VY
Sbjct: 448 AAYHVFRNVAQPFFNYIEEEDLLRFMIKEEVDLVFPLFDGAAETGRITRKAFTEWVVKVY 507

Query: 525 VERKSLAHSLNDTKTAIEELNKLASAVVLIVIIIEWLLLMGFLTTQVLVFISSQILLVVF 584
             R++LAHSLNDTKTA+++LNKL +A++++V ++ WLLL+   TT+VL+F S+Q++ + F
Sbjct: 508 TSRRALAHSLNDTKTAVKQLNKLVTAILMVVTVVIWLLLLEVATTKVLLFFSTQLVALAF 567

Query: 585 MFGNTARTVFEAIIFVFVMHPFDVGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNS 644
           + G+T + +FE+I+FVFVMHP+DVGDRCVVDGV M+VEEMN+LTT+FL+ +NEK++YPN+
Sbjct: 568 IIGSTCKNLFESIVFVFVMHPYDVGDRCVVDGVAMLVEEMNLLTTVFLKLNNEKVYYPNA 627

Query: 645 VLATKPISNYYRSPEMSDSIDFSVDFSTSIESIGALKARIKTYLESKPQFWRPNYSVVVK 704
           VLATKPISNY+RSP M ++++FS+ FST +  I  LK RI  YLE  PQ W P +SVVVK
Sbjct: 628 VLATKPISNYFRSPNMGETVEFSISFSTPVSKIAHLKERIAEYLEQNPQHWAPVHSVVVK 687

Query: 705 EIENVDKMKLSLCVNHTINFQNYGDKSNRRSDLVLELKKIFEDLGIKYHLLPQEVQL 752
           EIEN++K+K++L  +HTI FQ   +++ RR++L L +K++ EDL I Y LLPQ++ L
Sbjct: 688 EIENMNKLKMALYSDHTITFQENRERNLRRTELSLAIKRMLEDLHIDYTLLPQDINL 730

BLAST of PI0002153 vs. TAIR 10
Match: AT5G12080.3 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 675.2 bits (1741), Expect = 6.0e-194
Identity = 371/717 (51.74%), Postives = 504/717 (70.29%), Query Frame = 0

Query: 45  RSFRCTIPQSVVG----SSPSHEISRM--TPLKPPKIPGET---VTRRASFACSSFSKPK 104
           RS     P+S  G     SPS EIS++  +P KPP+ P +    +T+R SFA S +SKPK
Sbjct: 28  RSKEMASPESEKGVPFSKSPSPEISKLVGSPNKPPRAPNQNNVGLTQRKSFARSVYSKPK 87

Query: 105 SRLIESPCPDGASLAEEKALAKSTLYSSPKVDSPAKITTVTSPKEALKSTPITPKTPLIG 164
           SR ++  CP   S+ EE+   +     S    SP       S +      P+TP   ++ 
Sbjct: 88  SRFVDPSCPVDTSILEEEVREQLGAGFSFSRASPNN----KSNRSVGSPAPVTPSKVVV- 147

Query: 165 TTGNEEEDDEEVYKTAELKVKEKSGKRLKKTVLMEWVAFLCLTGCLIASLTIDKLVTKEI 224
               E+++DEE+YK  ++K+  +   ++    L+E   F+ +   L+ASLTI+ L     
Sbjct: 148 ----EKDEDEEIYK--KVKLNREMRSKISTLALIESAFFVVILSALVASLTINVLKHHTF 207

Query: 225 WGLGLWKWCVLVLVIFCGRLFSQWFINCLVFLIERNFLLKRKVLYFVYGLKKSVIIFIWL 284
           WGL +WKWCVLV+VIF G L + WF+  +VFLIE NFLL+RKVLYFV+GLKKSV +FIWL
Sbjct: 208 WGLEVWKWCVLVMVIFSGMLVTNWFMRLIVFLIETNFLLRRKVLYFVHGLKKSVQVFIWL 267

Query: 285 ALVLLAWGLLFDQSSKRSKKGNEILNYVTRALGASLIGAGLWLVKTLLVKILAASFQCTR 344
            L+L+AW LLF+   KRS    ++L  +TR L + L GA  WLVKTLL+KILAA+F    
Sbjct: 268 CLILVAWILLFNHDVKRSPAATKVLKCITRTLISILTGAFFWLVKTLLLKILAANFNVNN 327

Query: 345 FFDRIQESIFHQYILRILSGPPLMEMAERVGRAASTGQLSFRHLKKESDGGNEGKEEVID 404
           FFDRIQ+S+FHQY+L+ LSG PLME AERVGR  STG LSF  + K+   G   +++VID
Sbjct: 328 FFDRIQDSVFHQYVLQTLSGLPLMEEAERVGREPSTGHLSFATVVKK---GTVKEKKVID 387

Query: 405 VDKLKKMKQEKISAWTMRGLINVIRGSGLSTISNTI-ENFKEEEVEQKDKEINSEWEARA 464
           + K+ KMK+EK+SAWTMR L+  +R SGLSTIS+T+ E    E  EQ D+EI SE EA A
Sbjct: 388 MGKVHKMKREKVSAWTMRVLMEAVRTSGLSTISDTLDETAYGEGKEQADREITSEMEALA 447

Query: 465 AAYQIFRNVAKPGSKYIDEEDLFRFMSKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVY 524
           AAY +FRNVA+P   YI+EEDL RFM KEE+D V PLF+G AETG+I RK    W+V VY
Sbjct: 448 AAYHVFRNVAQPFFNYIEEEDLLRFMIKEEVDLVFPLFDGAAETGRITRKAFTEWVVKVY 507

Query: 525 VERKSLAHSLNDTKTAIEELNKLASAVVLIVIIIEWLLLMGFLTTQVLVFISSQILLVVF 584
             R++LAHSLNDTKTA+++LNKL +A++++V ++ WLLL+   TT+VL+F S+Q++ + F
Sbjct: 508 TSRRALAHSLNDTKTAVKQLNKLVTAILMVVTVVIWLLLLEVATTKVLLFFSTQLVALAF 567

Query: 585 MFGNTARTVFEAIIFVFVMHPFDVGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNS 644
           + G+T + +FE+I+FVFVMHP+DVGDRCVVDGV M+VEEMN+LTT+FL+ +NEK++YPN+
Sbjct: 568 IIGSTCKNLFESIVFVFVMHPYDVGDRCVVDGVAMLVEEMNLLTTVFLKLNNEKVYYPNA 627

Query: 645 VLATKPISNYYRSPEMSDSIDFSVDFSTSIESIGALKARIKTYLESKPQFWRPNYSVVVK 704
           VLATKPISNY+RSP M ++++FS+ FST +  I  LK RI  YLE  PQ W P +SVVVK
Sbjct: 628 VLATKPISNYFRSPNMGETVEFSISFSTPVSKIAHLKERIAEYLEQNPQHWAPVHSVVVK 687

Query: 705 EIENVDKMKLSLCVNHTINFQNYGDKSNRRSDLVLELKKIFEDLGIKYHLLPQEVQL 752
           EIEN++K+K++L  +HTI FQ   +++ RR++L L +K++ EDL I Y LLPQ++ L
Sbjct: 688 EIENMNKLKMALYSDHTITFQENRERNLRRTELSLAIKRMLEDLHIDYTLLPQDINL 730

BLAST of PI0002153 vs. TAIR 10
Match: AT5G19520.1 (mechanosensitive channel of small conductance-like 9 )

HSP 1 Score: 610.5 bits (1573), Expect = 1.8e-174
Identity = 355/752 (47.21%), Postives = 483/752 (64.23%), Query Frame = 0

Query: 13  RILEGEEGVHGSSKDLNKPSVGSFPDFDLKETRSFRCTIPQSVVG---SSPSHEISRMTP 72
           R+  GEE V   S   +     + P F+           P S  G   S P   IS  TP
Sbjct: 5   RVSNGEEVVINVSDKEDSKDPRASPSFN-------PLASPDSDAGIEKSKPVPPISIPTP 64

Query: 73  ---------LKPPKIPG-ETVTRRASFACSSFSKPKSRLIESPCPDGASLAEEKALAKST 132
                     KPPKIP  E + RR S + S +SKPKSR  E       S  EE    +S 
Sbjct: 65  EIYKFSGSVHKPPKIPSPEGLVRRKSLSRSIYSKPKSRFGEQQSFRYDSTREENG-GRSL 124

Query: 133 LYSSPKVDSPAKITTVTSPKEALKSTPITPKTPLIGTTGNEEEDDEEVYKTAELKVKEKS 192
                            SP    KS        L      E +++EE+YK  +L   ++S
Sbjct: 125 REQFGAGSFARGSFDRASPNN--KSNRSVASAALSKVAEEEPDENEEIYKKVKLHRVKRS 184

Query: 193 GKRLKKTVLMEWVAFLCLTGCLIASLTIDKLVTKEIWGLGLWKWCVLVLVIFCGRLFSQW 252
           G  +K    +E V F+ + G LI SLTID +    IWGL  WKWCVLV+V   G L + W
Sbjct: 185 G--MKPLAFLELVVFMAILGALIVSLTIDVVNKHTIWGLEFWKWCVLVMVTLSGMLVTNW 244

Query: 253 FINCLVFLIERNFLLKRKVLYFVYGLKKSVIIFIWLALVLLAWGLLFDQSSKRSKKGNEI 312
           F++ +VF+IE+N+LL++KVLYFV+GLKK+V +FIW +LVL+AW  LFD   KR++K    
Sbjct: 245 FMHFVVFIIEKNYLLRKKVLYFVHGLKKNVQVFIWFSLVLIAWICLFDGDVKRTRKTKRF 304

Query: 313 LNYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLM 372
           L+++T  + + L+G+ L+LVKT  +K+LA+ F    FF+RIQES+FHQY+L+ LSGPPL+
Sbjct: 305 LDFITWTIVSLLVGSILFLVKTFALKVLASKFNVRNFFERIQESVFHQYVLQTLSGPPLI 364

Query: 373 EMAERVGRAASTGQLSFRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVI 432
           E AE VGR  STG LSF   K     G    ++VID+ K+ +MKQEK+SAWTMR LI  +
Sbjct: 365 EEAENVGRVPSTGHLSFTRTK----DGKVKDKKVIDMGKVHRMKQEKVSAWTMRVLIEAV 424

Query: 433 RGSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRF 492
             SG+STIS+T++    ++ E+ DKEI +E EA AAAY +F NVAKP   YI+E+DL RF
Sbjct: 425 GTSGISTISSTLDEVNNKK-ERTDKEITNEMEAVAAAYDVFNNVAKPNHNYIEEDDLLRF 484

Query: 493 MSKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLAS 552
           M KEE+D VLPL E  A+TGKI RKT   W+VNVY  RK++ HSLNDTKTA+++L+KL +
Sbjct: 485 MIKEEVDLVLPLIE-DADTGKITRKTFTEWVVNVYTSRKTIGHSLNDTKTAVKQLDKLIT 544

Query: 553 AVVLIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVG 612
            ++ ++  I W++L+   +T++L+  SSQ L + FM G+T + +FE+ +FVFVMHP+DVG
Sbjct: 545 GILTVITFIVWMVLLDIASTKLLLVFSSQFLGLAFMIGSTCKNIFESFMFVFVMHPYDVG 604

Query: 613 DRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRSPEMSDSIDFSVD 672
           DRCVVDGV ++VEE+++LTT+FL+ DNEK+FYPNSVL +KPISN+YRSP+M D +DF + 
Sbjct: 605 DRCVVDGVMLLVEEIDLLTTVFLKIDNEKVFYPNSVLISKPISNFYRSPDMGDYVDFGIA 664

Query: 673 FSTSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVDKMKLSLCVNHTINFQNYGD 732
           FST  E IG LK +I  YL +  Q W P   V+V+ IEN++K+ L++ V HTINFQ Y +
Sbjct: 665 FSTPAEKIGCLKGKIGEYLVANSQHWYPEAQVMVRAIENMNKLVLNILVQHTINFQVYVE 724

Query: 733 KSNRRSDLVLELKKIFEDLGIKYHLLPQEVQL 752
           KS RR+ L++ +K+I EDL I Y LLPQ+V L
Sbjct: 725 KSLRRTALIIAIKRILEDLEIDYTLLPQDVNL 738

BLAST of PI0002153 vs. TAIR 10
Match: AT1G53470.1 (mechanosensitive channel of small conductance-like 4 )

HSP 1 Score: 516.2 bits (1328), Expect = 4.7e-146
Identity = 314/790 (39.75%), Postives = 466/790 (58.99%), Query Frame = 0

Query: 21  VHGSSKDLNKPSVGSFPD--FDLKETRSFRCTIPQSVV-GSSPSHEISRMTPLKPPKIPG 80
           V GS+  +  P   S+ +   D    R  R T+  SV  G S   E +R+   +  K   
Sbjct: 112 VSGSASPVTAPVTASYRNGTGDAIRRRQNRVTLSPSVKDGDSSEDEENRVDGSEVVKCTS 171

Query: 81  ETVTRRASFACSSFSKPKSRLIESPCPDGASLAEEKALAKSTLYSSPKVDSPAKITTVTS 140
              T R         K +SRL++ P P    +   +      L                +
Sbjct: 172 NRSTMRTKTLMK--MKTRSRLMDPPTPTYPDMVSGRTPRSGNL----------------N 231

Query: 141 PKEALKSTPITPKTPLIGTTGNEEEDDEEVYKTAELKVKEKSGKRLKKT---VLMEWVAF 200
           P  + ++T   P TP  G +  + E++E+ +   +L      G R +K    V++EW+  
Sbjct: 232 PGFSGRNT--KPGTPNQGGS-KDLEEEEDPFSEEDL----PEGLRKEKICVWVIIEWIFL 291

Query: 201 LCLTGCLIASLTIDKLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWFINCLVFLIERNFLL 260
           + +   LI SL I  L  K +W L LWKW V+VLV+ CGRL S W +   V+ +E NFL 
Sbjct: 292 ILIIASLICSLVIPYLRGKTLWDLALWKWEVMVLVLICGRLVSSWIVKLFVYFVESNFLW 351

Query: 261 KRKVLYFVYGLKKSVIIFIWLALVLLAWGLLFDQSSKRSKKGNEILNYVTRALGASLIGA 320
           ++KVLYFVYG++K V   +WL LVL+AW  LFD+  +R  + + +L YVT+ L   L+  
Sbjct: 352 RKKVLYFVYGIRKPVQNCLWLGLVLIAWHFLFDKKVEREMR-STVLKYVTKVLICLLVAV 411

Query: 321 GLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLMEM---AERVGRAAST 380
            +WL+KTLLVK+LA+SF  + +FDRIQES+F QY++  LSGPP +E+    E+V     T
Sbjct: 412 IIWLIKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPRIEIHIEEEKVANDVKT 471

Query: 381 GQLSFRHLK---------------------------------KESDGGNEGKEEVIDVDK 440
            ++  R L                                    S    EG EE I +D 
Sbjct: 472 FEIVGRKLSPLGPKAVSSPPQVTVGSGRLQKSPSRVGKSPVLSRSGSKKEGGEEGIRIDH 531

Query: 441 LKKMKQEKISAWTMRGLINVIRGSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQ 500
           L++M  + +SAW M+ L+NVI+   LST+   I++   +E + K  +I SE+EA+ AA +
Sbjct: 532 LQRMNTKNVSAWKMKKLMNVIKKGTLSTLDEQIQDTTTQE-DDKATQIRSEFEAKLAARK 591

Query: 501 IFRNVAKPGSKYIDEEDLFRFMSKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERK 560
           IF+NVA+PGS+YI  ED  RF+S++E +  + LFEG +E  KI +  LKNW+VN + ER+
Sbjct: 592 IFQNVAEPGSRYIYMEDFMRFLSEDESERAMDLFEGASECHKISKSCLKNWVVNAFRERR 651

Query: 561 SLAHSLNDTKTAIEELNKLASAVVLIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGN 620
           +LA +LNDTKTA+  L+++   +V IVI+I WLL++G  TT+ L+ ISSQ+LLVVF+FGN
Sbjct: 652 ALALTLNDTKTAVNRLHRIVDVLVSIVILIIWLLILGIATTKFLLVISSQLLLVVFVFGN 711

Query: 621 TARTVFEAIIFVFVMHPFDVGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLAT 680
           + +T+FEA+IFVFVMHPFDVGDRC +DGVQMIVEEMNILTT+FLR+DN+KI YPNS+L T
Sbjct: 712 SCKTIFEAVIFVFVMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQKIVYPNSLLGT 771

Query: 681 KPISNYYRSPEMSDSIDFSVDFSTSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIEN 740
           KPI+NYYRSP+M D+I+F V  +T  E   AL+ RI +Y+++K   W P+  +V +++  
Sbjct: 772 KPIANYYRSPDMQDAIEFFVHIATPPEKTTALRQRILSYVDNKKDHWHPSPMIVFRDMCG 831

Query: 741 VDKMKLSLCVNHTINFQNYGDKSNRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVSSAA 765
           ++ +K+++   H +N QN G++  RR  L+ E+ ++  +L I+Y L P  + +  + +A 
Sbjct: 832 LNSVKIAMWPTHKMNHQNMGERYVRRGQLLEEIGRLCRELDIEYRLYPLNINVKSLPAAT 874

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LYG98.4e-19351.74Mechanosensitive ion channel protein 10 OS=Arabidopsis thaliana OX=3702 GN=MSL10... [more]
Q84M972.6e-17347.21Mechanosensitive ion channel protein 9 OS=Arabidopsis thaliana OX=3702 GN=MSL9 P... [more]
Q9LPG36.5e-14539.75Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana OX=3702 GN=MSL4 P... [more]
Q9LH746.5e-14541.81Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana OX=3702 GN=MSL5 P... [more]
Q9SYM11.2e-14142.40Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana OX=3702 GN=MSL6 P... [more]
Match NameE-valueIdentityDescription
A0A1S3BR080.0e+0096.88Mechanosensitive ion channel protein OS=Cucumis melo OX=3656 GN=LOC103492355 PE=... [more]
A0A5D3CFQ70.0e+0096.88Mechanosensitive ion channel protein OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A5A7UIR30.0e+0096.74Mechanosensitive ion channel protein OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A0A0LYZ90.0e+0095.95Mechanosensitive ion channel protein OS=Cucumis sativus OX=3659 GN=Csa_1G595830 ... [more]
A0A6J1JDR10.0e+0087.92Mechanosensitive ion channel protein OS=Cucurbita maxima OX=3661 GN=LOC111484882... [more]
Match NameE-valueIdentityDescription
XP_008450905.10.0e+0096.88PREDICTED: mechanosensitive ion channel protein 10-like [Cucumis melo] >XP_00845... [more]
KAA0055793.10.0e+0096.74mechanosensitive ion channel protein 10-like [Cucumis melo var. makuwa][more]
XP_031738755.10.0e+0095.95mechanosensitive ion channel protein 10 [Cucumis sativus] >KGN66279.1 hypothetic... [more]
XP_038878911.10.0e+0093.99mechanosensitive ion channel protein 10-like [Benincasa hispida][more]
XP_022987285.10.0e+0087.92mechanosensitive ion channel protein 10-like [Cucurbita maxima] >XP_022987286.1 ... [more]
Match NameE-valueIdentityDescription
AT5G12080.16.0e-19451.74mechanosensitive channel of small conductance-like 10 [more]
AT5G12080.26.0e-19451.74mechanosensitive channel of small conductance-like 10 [more]
AT5G12080.36.0e-19451.74mechanosensitive channel of small conductance-like 10 [more]
AT5G19520.11.8e-17447.21mechanosensitive channel of small conductance-like 9 [more]
AT1G53470.14.7e-14639.75mechanosensitive channel of small conductance-like 4 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006685Mechanosensitive ion channel MscSPFAMPF00924MS_channelcoord: 536..741
e-value: 1.3E-23
score: 83.6
IPR023408Mechanosensitive ion channel MscS domain superfamilyGENE3D2.30.30.60coord: 595..644
e-value: 5.2E-12
score: 47.5
IPR016688Mechanosensitive ion channel MscS-like, plants/fungiPIRSFPIRSF017209Memb_At2g17000coord: 20..764
e-value: 2.5E-216
score: 718.8
IPR016688Mechanosensitive ion channel MscS-like, plants/fungiPANTHERPTHR31618MECHANOSENSITIVE ION CHANNEL PROTEIN 5coord: 4..757
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 138..155
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 138..161
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 53..77
NoneNo IPR availablePANTHERPTHR31618:SF7MECHANOSENSITIVE ION CHANNEL PROTEINcoord: 4..757
IPR010920LSM domain superfamilySUPERFAMILY50182Sm-like ribonucleoproteinscoord: 585..644

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0002153.1PI0002153.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006811 ion transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane