PI0001851 (gene) Melon (PI 482460) v1

Overview
NamePI0001851
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionGTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic
Locationchr05: 22545298 .. 22548314 (+)
RNA-Seq ExpressionPI0001851
SyntenyPI0001851
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTCTAATGTCTTTTATCAGCTTAAACCCTAAACCCTGTGAAGTGAAGAAGAGATGGAAAGACAGGAAAAATGTTGATTACTCGAAAGCTTTCTGCTTCAAACCTCAAATCCTTTCTTTCTCTGCGTTTCCTTTCTCACTTGTCTTCTTCACCTACCTGCTCAAACCCACCAATCCTTTTCCCACTTCCTTTCACTCTATCCCGTTCTTTCTCTTCCAAACCCACTCCTCCACAAGCTTCGAATCTCTCCCGTGATGGCAACTTTGACGAAACCACTTCCCGATTCTCCCTCGTCTGCCCTGGCTGTGGAGTTCATATGCAAGACTCCAATCCCAAGCATCCTGGCTTCTTCCTCAAACCCTCCAAAAAAGATTCCAATTATCGTCTCCTTACCCATCTCGTCCCCGTAGATGATGAATCTGAATGCTCTGATTTTCTCAAGAGAGGCCTCGTAATCGAGCCTGAAAAGGAGAAGACTGAAGAGGATGTGATCGAGAAACCCCAGAAGCCGACCGTGTGTTCGCGATGCCACTCGTTAAGGCATTATGGGAAGGTAAAGGATCCTTCAGTGGAGAATTTACTGCCGGATTTTGATTTCGATCATACTATCGGTAGGAGATTGGTGTCAACTACGGGAGCCCGATCTGTTGTTCTAATCGTCGTTGATGCTGCAGATTTTGATGGGTCATTCCCTAAAAAGGTAGCGAACTTGGTATCGGCGAGTATTGAGCATAATTCAGCTGCCTGGAAACAGGGGAAATCAGGGAATGTGCCAAGAGTGGTGCTTGTGGTGACGAAAATCGATTTGCTGCCTAGTTCTTTATCACCGACGGGATTCGAGCATTGGGTGAGGCAGAGAGCGAGAGAGGGAGGAATCAACAAGATAACGAGTTTGCATATGGTGAGTTCAATTAGAGATTGGGGGATGAAAAATTTGGTGGAGGATGTAATTGAGTTGGCTGGGGCGAGAGGACATGTGTGGGCAATTGGAGCACAAAATGCTGGGAAAAGCACGTTGATTAATTCAATAGGAAAGCATGTTGGTGGTAAGATCACGCAGTTGACTGAAGCACCAGTTCCTGGGACAACGTTGGGGATAATCAGAGTGGAGGGAATTTTTCCTGGTCAAGCCAAATTGTTTGATACTCCTGGCCTTCTCAATCCTCATCAGATTACTACAAGATTGACAAGGGAAGAGCAGAAACTTGTCCACATCAGCAAGGAATTGAAGCCCAGGACTTACAGGATTAAGGTAATGAAGTTGATCTTTTTTATGATCCTTTCTCATGCCTGAAACTTAATTGGTGCTTCATTACCATTGAACCTTCGTTCTTTAAGTTTTAGTTTCAGAGAAGGTTGGGTTTTAGGATGAGTAGGCTATTGTTTTCAGCCTGGTTATGCGCATCTTAACTAAACTTACTAGATAACCTGCCAAACCCTACAACATTTAAAGGTTAAGAGAACTTTCTAGGATATTAAATACTATGCATGTGGCCTTTCTAGATCGTGTGCTCTTATTGACCCCTAGATTAACGAATATGGTTTAGGAGGCTGTGTTATTGACTACGAAGATACATGATATAACCAACATGAAAAACCATAACAAAAGATCACGTGATTATTGAGTTTCGTTATGTTGTGAAATTGTGATTGGCTAAATAGAAAAATGGTATAATACTATATAATGATTTTAAAATTGTGAGTATTAAGTTTCTACTACCAATTTAATCTTCTATAGACAAATATTTTCAGCAAGAAATGTGGTTAGAGATTTCTTAATTTTGGTGTTACTTTTTACAGGTTGGTCATACAATTCATGTTGCTGGTCTCATGAGGCTAGATGTGGAAGAAACAAATGTGGATACAATTTATGTTACAGTTTGGGCATCTCCTTATCTTCCATTACACATGGGGAAAACAGAAAATGCAACCAAAATACAAGAGGATCATTTTGGGAATCATTTGCAGGTATTGTTTTGCACTAATTAGTTTGATGATAAACATACAGGATGGTCATTTTGTGGATATTCATTGTTTCTTGCTTTGCTTCAGCCACCTATAGGCAAAGATCGAGTAGCAGAACTTGGAAAATGGGTGAGAAGGGAATTTCATGTTTGTGGAACTAGTTGGGATTCAAGTTGTGTAGATGCTGCTGCTGCTGGCCTGGGTTGGTTTGCCATTGGACTAAAAGGCGAAGCAGTCTTAAACATATGGACTTATGAGGGGGTTGAGATTGTTTTACGAAGTTCTGTCATTCCTTTTCGATCAAACTTCTTTGAAGATGCTGGATTTACAGTCTCGAAAATTGTCTCTAAGGCTGATAAGGCTGCCAATAAACCACTTGGTCAAAGTGAAAAGAAGAAAAAGGGTGACCCCAAGGTTCATGTCCTTGCTAAAACGTAAGCATTGAGAGCTCACTTCGACTATGATTTTGCTTAAATGTTGTAGCATTGCACAGTTAGTGATCTACATCAATGTACAAGAATGGGGTTTGTCGGAATTTATGTTGGGGAATAGCAATGCGAGCATCTTCAAGCCTTGTCTTTCAGCCGGTGACAAGTGTCCGGGATTCGTGTGACTTCCTTCTCCAATATGTTTACTTCATCTTCATGATCTATGAAGCCCTGTTCACGCTCCAAACTCAAAGTAGGTTGCATCATATCAGAGAATGATATACTTAAATCTACTGCTTCATTTTCAGGGAAGGCTTTAGCTTCTATATTCATTACGGGAGGCAGTGCAACAACACCTAGAATGGATAGTAATTATAAACTTGAGGCATTGATTGTTTCCTCTACGGATTCGCAGCCCCAAAATGTCGAGAGTTGTGGTATTTTTCCTTTTTGTAGTCAGGTTGCCCATGTCTGATTAACATCCATCAAGATTAGTTCCTAGTCTTTCTATTTTTTCTATGTTTTTTCTATTCATTTGCAATAACCAGACTTCATATTTATAATATAAGTTGAAATTGATTTGATTATGTTTTCTCTTTGGTATAAACAATATGCATTTGACGAT

mRNA sequence

GTCTAATGTCTTTTATCAGCTTAAACCCTAAACCCTGTGAAGTGAAGAAGAGATGGAAAGACAGGAAAAATGTTGATTACTCGAAAGCTTTCTGCTTCAAACCTCAAATCCTTTCTTTCTCTGCGTTTCCTTTCTCACTTGTCTTCTTCACCTACCTGCTCAAACCCACCAATCCTTTTCCCACTTCCTTTCACTCTATCCCGTTCTTTCTCTTCCAAACCCACTCCTCCACAAGCTTCGAATCTCTCCCGTGATGGCAACTTTGACGAAACCACTTCCCGATTCTCCCTCGTCTGCCCTGGCTGTGGAGTTCATATGCAAGACTCCAATCCCAAGCATCCTGGCTTCTTCCTCAAACCCTCCAAAAAAGATTCCAATTATCGTCTCCTTACCCATCTCGTCCCCGTAGATGATGAATCTGAATGCTCTGATTTTCTCAAGAGAGGCCTCGTAATCGAGCCTGAAAAGGAGAAGACTGAAGAGGATGTGATCGAGAAACCCCAGAAGCCGACCGTGTGTTCGCGATGCCACTCGTTAAGGCATTATGGGAAGGTAAAGGATCCTTCAGTGGAGAATTTACTGCCGGATTTTGATTTCGATCATACTATCGGTAGGAGATTGGTGTCAACTACGGGAGCCCGATCTGTTGTTCTAATCGTCGTTGATGCTGCAGATTTTGATGGGTCATTCCCTAAAAAGGTAGCGAACTTGGTATCGGCGAGTATTGAGCATAATTCAGCTGCCTGGAAACAGGGGAAATCAGGGAATGTGCCAAGAGTGGTGCTTGTGGTGACGAAAATCGATTTGCTGCCTAGTTCTTTATCACCGACGGGATTCGAGCATTGGGTGAGGCAGAGAGCGAGAGAGGGAGGAATCAACAAGATAACGAGTTTGCATATGGTGAGTTCAATTAGAGATTGGGGGATGAAAAATTTGGTGGAGGATGTAATTGAGTTGGCTGGGGCGAGAGGACATGTGTGGGCAATTGGAGCACAAAATGCTGGGAAAAGCACGTTGATTAATTCAATAGGAAAGCATGTTGGTGGTAAGATCACGCAGTTGACTGAAGCACCAGTTCCTGGGACAACGTTGGGGATAATCAGAGTGGAGGGAATTTTTCCTGGTCAAGCCAAATTGTTTGATACTCCTGGCCTTCTCAATCCTCATCAGATTACTACAAGATTGACAAGGGAAGAGCAGAAACTTGTCCACATCAGCAAGGAATTGAAGCCCAGGACTTACAGGATTAAGGTTGGTCATACAATTCATGTTGCTGGTCTCATGAGGCTAGATGTGGAAGAAACAAATGTGGATACAATTTATGTTACAGTTTGGGCATCTCCTTATCTTCCATTACACATGGGGAAAACAGAAAATGCAACCAAAATACAAGAGGATCATTTTGGGAATCATTTGCAGCCACCTATAGGCAAAGATCGAGTAGCAGAACTTGGAAAATGGGTGAGAAGGGAATTTCATGTTTGTGGAACTAGTTGGGATTCAAGTTGTGTAGATGCTGCTGCTGCTGGCCTGGGTTGGTTTGCCATTGGACTAAAAGGCGAAGCAGTCTTAAACATATGGACTTATGAGGGGGTTGAGATTGTTTTACGAAGTTCTGTCATTCCTTTTCGATCAAACTTCTTTGAAGATGCTGGATTTACAGTCTCGAAAATTGTCTCTAAGGCTGATAAGGCTGCCAATAAACCACTTGGTCAAAGTGAAAAGAAGAAAAAGGGTGACCCCAAGGTTCATGTCCTTGCTAAAACGTAAGCATTGAGAGCTCACTTCGACTATGATTTTGCTTAAATGTTGTAGCATTGCACAGTTAGTGATCTACATCAATGTACAAGAATGGGGTTTGTCGGAATTTATGTTGGGGAATAGCAATGCGAGCATCTTCAAGCCTTGTCTTTCAGCCGGTGACAAGTGTCCGGGATTCGTGTGACTTCCTTCTCCAATATGTTTACTTCATCTTCATGATCTATGAAGCCCTGTTCACGCTCCAAACTCAAAGTAGGTTGCATCATATCAGAGAATGATATACTTAAATCTACTGCTTCATTTTCAGGGAAGGCTTTAGCTTCTATATTCATTACGGGAGGCAGTGCAACAACACCTAGAATGGATAGTAATTATAAACTTGAGGCATTGATTGTTTCCTCTACGGATTCGCAGCCCCAAAATGTCGAGAGTTGTGGTATTTTTCCTTTTTGTAGTCAGGTTGCCCATGTCTGATTAACATCCATCAAGATTAGTTCCTAGTCTTTCTATTTTTTCTATGTTTTTTCTATTCATTTGCAATAACCAGACTTCATATTTATAATATAAGTTGAAATTGATTTGATTATGTTTTCTCTTTGGTATAAACAATATGCATTTGACGAT

Coding sequence (CDS)

ATGTTGATTACTCGAAAGCTTTCTGCTTCAAACCTCAAATCCTTTCTTTCTCTGCGTTTCCTTTCTCACTTGTCTTCTTCACCTACCTGCTCAAACCCACCAATCCTTTTCCCACTTCCTTTCACTCTATCCCGTTCTTTCTCTTCCAAACCCACTCCTCCACAAGCTTCGAATCTCTCCCGTGATGGCAACTTTGACGAAACCACTTCCCGATTCTCCCTCGTCTGCCCTGGCTGTGGAGTTCATATGCAAGACTCCAATCCCAAGCATCCTGGCTTCTTCCTCAAACCCTCCAAAAAAGATTCCAATTATCGTCTCCTTACCCATCTCGTCCCCGTAGATGATGAATCTGAATGCTCTGATTTTCTCAAGAGAGGCCTCGTAATCGAGCCTGAAAAGGAGAAGACTGAAGAGGATGTGATCGAGAAACCCCAGAAGCCGACCGTGTGTTCGCGATGCCACTCGTTAAGGCATTATGGGAAGGTAAAGGATCCTTCAGTGGAGAATTTACTGCCGGATTTTGATTTCGATCATACTATCGGTAGGAGATTGGTGTCAACTACGGGAGCCCGATCTGTTGTTCTAATCGTCGTTGATGCTGCAGATTTTGATGGGTCATTCCCTAAAAAGGTAGCGAACTTGGTATCGGCGAGTATTGAGCATAATTCAGCTGCCTGGAAACAGGGGAAATCAGGGAATGTGCCAAGAGTGGTGCTTGTGGTGACGAAAATCGATTTGCTGCCTAGTTCTTTATCACCGACGGGATTCGAGCATTGGGTGAGGCAGAGAGCGAGAGAGGGAGGAATCAACAAGATAACGAGTTTGCATATGGTGAGTTCAATTAGAGATTGGGGGATGAAAAATTTGGTGGAGGATGTAATTGAGTTGGCTGGGGCGAGAGGACATGTGTGGGCAATTGGAGCACAAAATGCTGGGAAAAGCACGTTGATTAATTCAATAGGAAAGCATGTTGGTGGTAAGATCACGCAGTTGACTGAAGCACCAGTTCCTGGGACAACGTTGGGGATAATCAGAGTGGAGGGAATTTTTCCTGGTCAAGCCAAATTGTTTGATACTCCTGGCCTTCTCAATCCTCATCAGATTACTACAAGATTGACAAGGGAAGAGCAGAAACTTGTCCACATCAGCAAGGAATTGAAGCCCAGGACTTACAGGATTAAGGTTGGTCATACAATTCATGTTGCTGGTCTCATGAGGCTAGATGTGGAAGAAACAAATGTGGATACAATTTATGTTACAGTTTGGGCATCTCCTTATCTTCCATTACACATGGGGAAAACAGAAAATGCAACCAAAATACAAGAGGATCATTTTGGGAATCATTTGCAGCCACCTATAGGCAAAGATCGAGTAGCAGAACTTGGAAAATGGGTGAGAAGGGAATTTCATGTTTGTGGAACTAGTTGGGATTCAAGTTGTGTAGATGCTGCTGCTGCTGGCCTGGGTTGGTTTGCCATTGGACTAAAAGGCGAAGCAGTCTTAAACATATGGACTTATGAGGGGGTTGAGATTGTTTTACGAAGTTCTGTCATTCCTTTTCGATCAAACTTCTTTGAAGATGCTGGATTTACAGTCTCGAAAATTGTCTCTAAGGCTGATAAGGCTGCCAATAAACCACTTGGTCAAAGTGAAAAGAAGAAAAAGGGTGACCCCAAGGTTCATGTCCTTGCTAAAACGTAA

Protein sequence

MLITRKLSASNLKSFLSLRFLSHLSSSPTCSNPPILFPLPFTLSRSFSSKPTPPQASNLSRDGNFDETTSRFSLVCPGCGVHMQDSNPKHPGFFLKPSKKDSNYRLLTHLVPVDDESECSDFLKRGLVIEPEKEKTEEDVIEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDFDHTIGRRLVSTTGARSVVLIVVDAADFDGSFPKKVANLVSASIEHNSAAWKQGKSGNVPRVVLVVTKIDLLPSSLSPTGFEHWVRQRAREGGINKITSLHMVSSIRDWGMKNLVEDVIELAGARGHVWAIGAQNAGKSTLINSIGKHVGGKITQLTEAPVPGTTLGIIRVEGIFPGQAKLFDTPGLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVEETNVDTIYVTVWASPYLPLHMGKTENATKIQEDHFGNHLQPPIGKDRVAELGKWVRREFHVCGTSWDSSCVDAAAAGLGWFAIGLKGEAVLNIWTYEGVEIVLRSSVIPFRSNFFEDAGFTVSKIVSKADKAANKPLGQSEKKKKGDPKVHVLAKT
Homology
BLAST of PI0001851 vs. ExPASy Swiss-Prot
Match: Q8W4I6 (GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BPG2 PE=1 SV=1)

HSP 1 Score: 448.7 bits (1153), Expect = 9.5e-125
Identity = 235/575 (40.87%), Postives = 344/575 (59.83%), Query Frame = 0

Query: 53  PPQASNLSRDGNFDETTSRFSLVCPGCGVHMQDSNPKHPGFFLKPSKKDSNYRLLTHL-- 112
           P ++ NL      DE      ++CPGCG+ MQD++P  PG++ K     +N     H+  
Sbjct: 75  PKKSKNLIMSEGRDEDEDYGKIICPGCGIFMQDNDPDLPGYYQKRKVIANNLEGDEHVEN 134

Query: 113 ------VPVDDES---------ECSDFLKRGLV--------------------------- 172
                   VDD++         E  D +K  +                            
Sbjct: 135 DELAGFEMVDDDADEEEEGEDDEMDDEIKNAIEGSNSESESGFEWESDEWEEKKEVNDVE 194

Query: 173 -------------IEPEKE------KTEEDVIEKPQKP-------TVCSRCHSLRHYGKV 232
                        +  EKE      KTE   I + +         TVC+RCHSLR+YG+V
Sbjct: 195 LDGFAPAGVGYGNVTEEKEKKKRVSKTERKKIAREEAKKDNYDDVTVCARCHSLRNYGQV 254

Query: 233 KDPSVENLLPDFDFDHTIGRRLVS--TTGARSVVLIVVDAADFDGSFPKKVANLVSASIE 292
           K+ + ENLLPDFDFD  I  RL+   +  + +VV++VVD  DFDGSFPK+ A  +   ++
Sbjct: 255 KNQAAENLLPDFDFDRLISTRLIKPMSNSSTTVVVMVVDCVDFDGSFPKRAAKSLFQVLQ 314

Query: 293 HNSAAWKQGKSGNVPRVVLVVTKIDLLPSSLSPTGFEHWVRQRAREGGINKITSLHMVSS 352
                 K  K  N+P++VLV TK+DLLP+ +SP   + WVR RA+ GG  K++ ++MVS+
Sbjct: 315 KAENDPKGSK--NLPKLVLVATKVDLLPTQISPARLDRWVRHRAKAGGAPKLSGVYMVSA 374

Query: 353 IRDWGMKNLVEDVIELAGARGHVWAIGAQNAGKSTLINSIGKHVGGKITQLTEAPVPGTT 412
            +D G+KNL+  + ELAG RG+VW IGAQNAGKSTLIN++ K  G K+T+LTEAPVPGTT
Sbjct: 375 RKDIGVKNLLAYIKELAGPRGNVWVIGAQNAGKSTLINALSKKDGAKVTRLTEAPVPGTT 434

Query: 413 LGIIRVEGIFPGQAKLFDTPGLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIH 472
           LGI+++ GI   +AK++DTPGLL+P+ ++ RL  EE+K+V I KE++PR+YR+K G ++H
Sbjct: 435 LGILKIGGILSAKAKMYDTPGLLHPYLMSLRLNSEERKMVEIRKEVQPRSYRVKAGQSVH 494

Query: 473 VAGLMRLDVEETNVDTIYVTVWASPYLPLHMGKTENATKIQEDHFGNHLQPPIGKDRVAE 532
           + GL+RLD+   +V+TIY+T+WAS  + LH+GKTENA +I + H G  LQPPIG++R +E
Sbjct: 495 IGGLVRLDLVSASVETIYITIWASHSVSLHLGKTENAEEIFKGHSGLRLQPPIGENRASE 554

Query: 533 LGKWVRREFHVCGTSWDSSCVDAAAAGLGWFAIGLKGEAVLNIWTYEGVEIVLRSSVIPF 556
           LG W  +E  V G SWD   +D + AGLGW ++GLKG A L +WTY+G+++ LR  ++  
Sbjct: 555 LGTWEEKEIQVSGNSWDVKSIDISVAGLGWLSLGLKGAATLALWTYQGIDVTLREPLVID 614

BLAST of PI0001851 vs. ExPASy Swiss-Prot
Match: P54453 (Uncharacterized protein YqeH OS=Bacillus subtilis (strain 168) OX=224308 GN=yqeH PE=1 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 3.6e-39
Identity = 127/405 (31.36%), Postives = 193/405 (47.65%), Query Frame = 0

Query: 124 KRGLVIEPEKEKTEEDVIEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDF---DHTI 183
           K GL   P    T+E+VI        C RC  L++Y +++D S    L D DF    H I
Sbjct: 18  KTGLGYAPPASLTKENVI--------CQRCFRLKNYNEIQDVS----LTDDDFLNILHGI 77

Query: 184 GRRLVSTTGARSVVLIVVDAADFDGSFPKKVANLVSASIEHNSAAWKQGKSGNVPRVVLV 243
           G          S+V+ +VD  DF+GS+   +  LV                GN   ++LV
Sbjct: 78  GE-------TDSLVVKIVDIFDFNGSWINGLQRLV---------------GGN--PILLV 137

Query: 244 VTKIDLLPSSLSPTGFEHWVRQRAREGGINKITSLHMVSSIRDWGMKNLVEDVIELAGAR 303
             K D+LP SL       W+++ A+E G+ K   + +VS+ R  G++ ++ D IE     
Sbjct: 138 GNKADILPKSLKRERLIQWMKREAKELGL-KPVDVFLVSAGRGQGIREVI-DAIEHYRNG 197

Query: 304 GHVWAIGAQNAGKSTLINSIGKHVGGKITQLTEAPVPGTTLGIIRVEGIFPGQAKLFDTP 363
             V+ +G  N GKST IN I K V G+   +T +  PGTTL  I +       + L+DTP
Sbjct: 198 KDVYVVGCTNVGKSTFINRIIKEVSGEEDIITTSQFPGTTLDAIEIP--LDDGSSLYDTP 257

Query: 364 GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVEETNVDTIYVT 423
           G++N HQ+   + +++ K++   KELKPRT+++    T++  GL R D         YV+
Sbjct: 258 GIINNHQMAHYVNKKDLKILSPKKELKPRTFQLNDQQTLYFGGLARFD---------YVS 317

Query: 424 VWASPY-------LPLHMGKTENATKIQEDHFGNHLQPPIGKDRVAELGKWVRREFHVCG 483
              SP+       L +H  K ENA  + E H G  L PP GKD + E  + V   F +  
Sbjct: 318 GERSPFICYMPNELMIHRTKLENADALYEKHAGELLTPP-GKDEMDEFPELVAHTFTI-- 366

Query: 484 TSWDSSCVDAAAAGLGWFAIGLKGEAVLNIWTYEGVEIVLRSSVI 519
                   D   +GLGW  +    +  +  +  +GV + +R S+I
Sbjct: 378 ---KDKKTDIVFSGLGWVTVH-DADKKVTAYAPKGVHVFVRRSLI 366

BLAST of PI0001851 vs. ExPASy Swiss-Prot
Match: Q66GP9 (NO-associated protein 1, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NOA1 PE=1 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 5.0e-25
Identity = 96/372 (25.81%), Postives = 161/372 (43.28%), Query Frame = 0

Query: 149 VCSRCHSLRHYGKVKDPSVENLLP---DFDFDHTIGRRLVSTTGARSVVLIVVDAADFDG 208
           +C RC  L H   +         P    F     +  +L      +++++ +VD  DF+G
Sbjct: 144 ICGRCQLLSHGHMITAVGGNGGYPGGKQFVSADELREKLSHLRHEKALIVKLVDIVDFNG 203

Query: 209 SFPKKVANLVSASIEHNSAAWKQGKSGNVPRVVLVVTKIDLLPSSLSPTGFEHWVRQRAR 268
           SF  +V +LV A+                  ++LV+TKIDLLP          WV +   
Sbjct: 204 SFLARVRDLVGAN-----------------PIILVITKIDLLPKGTDMNCIGDWVVEVTM 263

Query: 269 EGGINKITSLHMVSSIRDWGMKNLVEDVIELAGARGHVWAIGAQNAGKSTLINSIGKHV- 328
              +N + S+H+ SS    G+  +  ++ +    R  V+ +GA N GKS  IN++ K + 
Sbjct: 264 RKKLN-VLSVHLTSSKSLDGVSGVASEIQKEKKGR-DVYILGAANVGKSAFINALLKTMA 323

Query: 329 -------GGKITQLTEAPVPGTTLGIIRVEGIFPGQAKLFDTPGLLNPHQITTRLTREEQ 388
                    +  +  ++ VPGTTLG I++   F G  KL+DTPG+   H+    +  ++ 
Sbjct: 324 ERDPVAAAAQKYKPIQSAVPGTTLGPIQINA-FVGGEKLYDTPGVHLHHRQAAVVHSDDL 383

Query: 389 KLVHISKELKPRTYRIKV---------------GHTIHVAGLMRLDVEETNVDTIYVTVW 448
             +     L+ +++ I                 G+T    GL+R+D+ +   +T + T +
Sbjct: 384 PALAPQNRLRGQSFDISTLPTQSSSSPKGESLNGYTFFWGGLVRIDILKALPETCF-TFY 443

Query: 449 ASPYLPLHMGKTENATKIQEDHFGNHLQPPIGKDRVAE-LGKWVRREFHVCGTSWDSSCV 494
               L +H   T+ AT   E   G  L PP GK+++ E  G    R   +          
Sbjct: 444 GPKALEIHAVPTKTATAFYEKELGVLLTPPSGKNQMQEWKGLQSHRLLQIEINDAKRPAS 494

BLAST of PI0001851 vs. ExPASy Swiss-Prot
Match: Q6YPG5 (Putative nitric oxide synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0104700 PE=3 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 4.7e-23
Identity = 91/366 (24.86%), Postives = 162/366 (44.26%), Query Frame = 0

Query: 146 KPTVCSRCHSLRHYGKVKDPSVENLLP---DFDFDHTIGRRLVSTTGARSVVLIVVDAAD 205
           +  +C RC  L H   +         P    F     +  +L      +++++ +VD  D
Sbjct: 133 RTVLCGRCKLLSHGHMITAVGGHGGYPGGKQFVSADQLRDKLSYLRHEKALIIKLVDIVD 192

Query: 206 FDGSFPKKVANLVSASIEHNSAAWKQGKSGNVPRVVLVVTKIDLLPSSLSPTGFEHWVRQ 265
           F+GSF  +V +   A+                  ++LV+TK+DLLP          WV +
Sbjct: 193 FNGSFLARVRDFAGAN-----------------PIILVITKVDLLPRDTDLNCIGDWVVE 252

Query: 266 RAREGGINKITSLHMVSSIRDWGMKNLVEDVIELAGARGHVWAIGAQNAGKSTLINSIGK 325
              +  +N + S+H+ SS    G+  ++ ++ +    R  V+ +G+ N GKS  I+++ +
Sbjct: 253 AVVKKKLN-VLSVHLTSSKSLVGVTGVISEIQQEKKGR-DVYILGSANVGKSAFISAMLR 312

Query: 326 HVGGKIT--------QLTEAPVPGTTLGIIRVEGIFPGQAKLFDTPGLLNPHQITTRLTR 385
            +  K          +  ++ VPGTTLG I++E  F G  KL+DTPG+   H+    +  
Sbjct: 313 TMAYKDPVAAAAQKYKPIQSAVPGTTLGPIQIEA-FLGGGKLYDTPGVHLHHRQAAVIHA 372

Query: 386 EEQKLVHISKELKPRTYRIK------VGHTIHVAGLMRLDVEETNVDTIYVTVWASPYLP 445
           ++   +     L+ R +          G+++   GL+R+DV +  +    +T +    L 
Sbjct: 373 DDLPSLAPQSRLRARCFPANDTDVGLSGNSLFWGGLVRIDVVKA-LPRTRLTFYGPKKLK 432

Query: 446 LHMGKTENATKIQEDHFGNHLQPPIGKDRV-AELGKWVRREFHVCGTSWDSSCVDAAAAG 494
           ++M  T  A +  E   G  L PP GK++    +G    RE  +     D    D A +G
Sbjct: 433 INMVPTTEADEFYEREVGVTLTPPAGKEKAEGWVGLQGVRELQIKYEESDRPACDIAISG 477

BLAST of PI0001851 vs. ExPASy Swiss-Prot
Match: Q9JJG9 (Nitric oxide-associated protein 1 OS=Mus musculus OX=10090 GN=Noa1 PE=1 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 4.4e-13
Identity = 49/171 (28.65%), Postives = 83/171 (48.54%), Query Frame = 0

Query: 357 FDTPGLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVEETNVDT 416
           +DTPG+     I   LT +E   V  +  + PRT+ +K G  + + G+ R+D  + N  +
Sbjct: 496 YDTPGITKESCILNLLTEKEINTVLPTHSIIPRTFVLKPGMVLFLGGIARIDFLQGN-QS 555

Query: 417 IYVTVWASPYLPLHMGKTENATKIQEDHFGNH--LQPPIGKDRVAELGKWVRREFHVCGT 476
            + TV AS +LP+H+   + A  + E H G+   L P  GK+R+A+    V  +  + G 
Sbjct: 556 AWFTVVASNFLPVHITSLDKADALYEKHAGHELLLVPMGGKERMAQFPPLVAEDITLKGG 615

Query: 477 SWDSSCVDAAAAGLGWFAIGLKGEAVLNI--WTYEGVEIVLRSSVIPFRSN 524
               +  D   +  GW A+    E  L++   T EG  + +   V+P+  N
Sbjct: 616 GKFEAVADIKFSSAGWVAVTPYSEGTLHLRGHTPEGTALTVHPPVLPYIVN 665

BLAST of PI0001851 vs. ExPASy TrEMBL
Match: A0A0A0KZ58 (G domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G285700 PE=4 SV=1)

HSP 1 Score: 1064.3 bits (2751), Expect = 1.8e-307
Identity = 526/566 (92.93%), Postives = 544/566 (96.11%), Query Frame = 0

Query: 1   MLITRKLSASNLKSFLSLRFLSHLSSSPTCSNPPILFPLPFTLSRSFSSKPTPPQASNLS 60
           MLI RKLSASNLKSFL LRF+SHLSSSP CSNP ILFPLP TLSRSFSSKPT PQA +LS
Sbjct: 1   MLIARKLSASNLKSFLFLRFISHLSSSPICSNPSILFPLPSTLSRSFSSKPTSPQAFSLS 60

Query: 61  RDGNFDETTSRFSLVCPGCGVHMQDSNPKHPGFFLKPSKKDSNYRLLTHLVPVDDESECS 120
           RDGNFDETTSRFSLVCPGCGVHMQDSNPKHPGFF+KPSKKDSNYRLLTHLVPVDDESECS
Sbjct: 61  RDGNFDETTSRFSLVCPGCGVHMQDSNPKHPGFFIKPSKKDSNYRLLTHLVPVDDESECS 120

Query: 121 DFLKRGLVIEPEKEKTEEDVIEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDFDHTI 180
           +FLKRGLVIEPEKEKTEEDV EKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDF+HT+
Sbjct: 121 EFLKRGLVIEPEKEKTEEDVNEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDFNHTL 180

Query: 181 GRRLVSTTGARSVVLIVVDAADFDGSFPKKVANLVSASIEHNSAAWKQGKSGNVPRVVLV 240
           G RLVSTTG RSVVLIVVDAADFDGSFPKKVANLVSASIE+NSAAWKQGKSGNVPRVVLV
Sbjct: 181 GGRLVSTTGTRSVVLIVVDAADFDGSFPKKVANLVSASIENNSAAWKQGKSGNVPRVVLV 240

Query: 241 VTKIDLLPSSLSPTGFEHWVRQRAREGGINKITSLHMVSSIRDWGMKNLVEDVIELAGAR 300
           VTK DLLPSSLSP  FEHWVRQRAREGGINKITSLHMVSSIRDWG+KNLVEDVIELAGAR
Sbjct: 241 VTKTDLLPSSLSPEKFEHWVRQRAREGGINKITSLHMVSSIRDWGIKNLVEDVIELAGAR 300

Query: 301 GHVWAIGAQNAGKSTLINSIGKHVGGKITQLTEAPVPGTTLGIIRVEGIFPGQAKLFDTP 360
           G+VWAIGAQNAGKSTLINSIGKHVGGKITQLTEAPVPGTTLGIIRVEGIFP QAKLFDTP
Sbjct: 301 GNVWAIGAQNAGKSTLINSIGKHVGGKITQLTEAPVPGTTLGIIRVEGIFPAQAKLFDTP 360

Query: 361 GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVEETNVDTIYVT 420
           GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVEETN+DTIYVT
Sbjct: 361 GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVEETNLDTIYVT 420

Query: 421 VWASPYLPLHMGKTENATKIQEDHFGNHLQPPIGKDRVAELGKWVRREFHVCGTSWDSSC 480
           VWASPYLPLHMGKTENATK+QEDHFG  LQPPIGKDRV ELGKWVRREF VCGTSWDSSC
Sbjct: 421 VWASPYLPLHMGKTENATKMQEDHFGIQLQPPIGKDRVEELGKWVRREFRVCGTSWDSSC 480

Query: 481 VDAAAAGLGWFAIGLKGEAVLNIWTYEGVEIVLRSSVIPFRSNFFEDAGFTVSKIVSKAD 540
           VDAA AGLGWFAIGLKGE VL+IWTYEGV+IVLRSSVIP R+NFFEDAGFTVSKIVSKAD
Sbjct: 481 VDAAVAGLGWFAIGLKGEVVLSIWTYEGVDIVLRSSVIPHRANFFEDAGFTVSKIVSKAD 540

Query: 541 KAANKPLGQSEKKKKGDPKVHVLAKT 567
           +A+NKPLGQSEKKKK DP+VHVLA+T
Sbjct: 541 QASNKPLGQSEKKKKSDPEVHVLAQT 566

BLAST of PI0001851 vs. ExPASy TrEMBL
Match: A0A1S3BNH1 (GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103491566 PE=4 SV=1)

HSP 1 Score: 1055.0 bits (2727), Expect = 1.1e-304
Identity = 523/566 (92.40%), Postives = 542/566 (95.76%), Query Frame = 0

Query: 1   MLITRKLSASNLKSFLSLRFLSHLSSSPTCSNPPILFPLPFTLSRSFSSKPTPPQASNLS 60
           MLI RKLSASNLKSFLS RF+ HLSSSPTCSNP ILFPLP TL  SFSSKPTPPQA NLS
Sbjct: 1   MLIARKLSASNLKSFLSPRFIYHLSSSPTCSNPSILFPLPSTLFCSFSSKPTPPQALNLS 60

Query: 61  RDGNFDETTSRFSLVCPGCGVHMQDSNPKHPGFFLKPSKKDSNYRLLTHLVPVDDESECS 120
           RDGNFDETTSR S VCPGCGVHMQDSNPKHPGFF+KPSKKDS+YR LTHLVPVDDESECS
Sbjct: 61  RDGNFDETTSRLSHVCPGCGVHMQDSNPKHPGFFIKPSKKDSSYRRLTHLVPVDDESECS 120

Query: 121 DFLKRGLVIEPEKEKTEEDVIEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDFDHTI 180
           +FLKRGLVIEPEKEKTEEDVIEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDF HT+
Sbjct: 121 EFLKRGLVIEPEKEKTEEDVIEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDFKHTL 180

Query: 181 GRRLVSTTGARSVVLIVVDAADFDGSFPKKVANLVSASIEHNSAAWKQGKSGNVPRVVLV 240
           G RLVSTTG RSVVLIVVDAADFDGSFPKKVANLVSASIE+NSAAWKQGKSGNVPRVVLV
Sbjct: 181 GGRLVSTTGTRSVVLIVVDAADFDGSFPKKVANLVSASIENNSAAWKQGKSGNVPRVVLV 240

Query: 241 VTKIDLLPSSLSPTGFEHWVRQRAREGGINKITSLHMVSSIRDWGMKNLVEDVIELAGAR 300
           VTK DLLPSSLSPT FE WVRQRAREGGINKITSLHMVS+I+DWG+KNLVEDVIELAGAR
Sbjct: 241 VTKTDLLPSSLSPTTFERWVRQRAREGGINKITSLHMVSAIKDWGIKNLVEDVIELAGAR 300

Query: 301 GHVWAIGAQNAGKSTLINSIGKHVGGKITQLTEAPVPGTTLGIIRVEGIFPGQAKLFDTP 360
           G+VWAIGAQNAGKSTLINSIGKHVGGK+T LTEAPVPGTTLGIIRVEGIFP QAKLFDTP
Sbjct: 301 GNVWAIGAQNAGKSTLINSIGKHVGGKVTHLTEAPVPGTTLGIIRVEGIFPEQAKLFDTP 360

Query: 361 GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVEETNVDTIYVT 420
           GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDV+ETNVDTIYVT
Sbjct: 361 GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVDETNVDTIYVT 420

Query: 421 VWASPYLPLHMGKTENATKIQEDHFGNHLQPPIGKDRVAELGKWVRREFHVCGTSWDSSC 480
           VWASPYLPLHMGKTENATKI+EDHFG  LQPPIGKDRVAELGKWVR+EF VCGTSWDSSC
Sbjct: 421 VWASPYLPLHMGKTENATKIREDHFGIQLQPPIGKDRVAELGKWVRKEFRVCGTSWDSSC 480

Query: 481 VDAAAAGLGWFAIGLKGEAVLNIWTYEGVEIVLRSSVIPFRSNFFEDAGFTVSKIVSKAD 540
           VDAAAAGLGWF+IGLKGEAVL+IWTYEGVEIVLRSSVIP R+NFFEDAGFTVSKIVSKAD
Sbjct: 481 VDAAAAGLGWFSIGLKGEAVLSIWTYEGVEIVLRSSVIPDRANFFEDAGFTVSKIVSKAD 540

Query: 541 KAANKPLGQSEKKKKGDPKVHVLAKT 567
           +AANK LGQSEKKKKGDPKVH+LAKT
Sbjct: 541 QAANKQLGQSEKKKKGDPKVHMLAKT 566

BLAST of PI0001851 vs. ExPASy TrEMBL
Match: A0A5D3DDC5 (GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G00340 PE=4 SV=1)

HSP 1 Score: 1053.1 bits (2722), Expect = 4.1e-304
Identity = 522/566 (92.23%), Postives = 542/566 (95.76%), Query Frame = 0

Query: 1   MLITRKLSASNLKSFLSLRFLSHLSSSPTCSNPPILFPLPFTLSRSFSSKPTPPQASNLS 60
           MLI RKLSASNLKSFLS RF+ HLSSSPTCSNP ILFPLP TL  SFSSKPTPPQA NLS
Sbjct: 1   MLIARKLSASNLKSFLSPRFIYHLSSSPTCSNPSILFPLPSTLFCSFSSKPTPPQALNLS 60

Query: 61  RDGNFDETTSRFSLVCPGCGVHMQDSNPKHPGFFLKPSKKDSNYRLLTHLVPVDDESECS 120
           RDGNFDETTSR S VCPGCGVHMQDSNPKHPGFF+KPSKKDS+YR LTHLVPV+DESECS
Sbjct: 61  RDGNFDETTSRLSHVCPGCGVHMQDSNPKHPGFFIKPSKKDSSYRRLTHLVPVNDESECS 120

Query: 121 DFLKRGLVIEPEKEKTEEDVIEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDFDHTI 180
           +FLKRGLVIEPEKEKTEEDVIEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDF HT+
Sbjct: 121 EFLKRGLVIEPEKEKTEEDVIEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDFKHTL 180

Query: 181 GRRLVSTTGARSVVLIVVDAADFDGSFPKKVANLVSASIEHNSAAWKQGKSGNVPRVVLV 240
           G RLVSTTG RSVVLIVVDAADFDGSFPKKVANLVSASIE+NSAAWKQGKSGNVPRVVLV
Sbjct: 181 GGRLVSTTGTRSVVLIVVDAADFDGSFPKKVANLVSASIENNSAAWKQGKSGNVPRVVLV 240

Query: 241 VTKIDLLPSSLSPTGFEHWVRQRAREGGINKITSLHMVSSIRDWGMKNLVEDVIELAGAR 300
           VTK DLLPSSLSPT FE WVRQRAREGGINKITSLHMVS+I+DWG+KNLVEDVIELAGAR
Sbjct: 241 VTKTDLLPSSLSPTTFERWVRQRAREGGINKITSLHMVSAIKDWGIKNLVEDVIELAGAR 300

Query: 301 GHVWAIGAQNAGKSTLINSIGKHVGGKITQLTEAPVPGTTLGIIRVEGIFPGQAKLFDTP 360
           G+VWAIGAQNAGKSTLINSIGKHVGGK+T LTEAPVPGTTLGIIRVEGIFP QAKLFDTP
Sbjct: 301 GNVWAIGAQNAGKSTLINSIGKHVGGKVTHLTEAPVPGTTLGIIRVEGIFPEQAKLFDTP 360

Query: 361 GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVEETNVDTIYVT 420
           GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDV+ETNVDTIYVT
Sbjct: 361 GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVDETNVDTIYVT 420

Query: 421 VWASPYLPLHMGKTENATKIQEDHFGNHLQPPIGKDRVAELGKWVRREFHVCGTSWDSSC 480
           VWASPYLPLHMGKTENATKI+EDHFG  LQPPIGKDRVAELGKWVR+EF VCGTSWDSSC
Sbjct: 421 VWASPYLPLHMGKTENATKIREDHFGIQLQPPIGKDRVAELGKWVRKEFRVCGTSWDSSC 480

Query: 481 VDAAAAGLGWFAIGLKGEAVLNIWTYEGVEIVLRSSVIPFRSNFFEDAGFTVSKIVSKAD 540
           VDAAAAGLGWF+IGLKGEAVL+IWTYEGVEIVLRSSVIP R+NFFEDAGFTVSKIVSKAD
Sbjct: 481 VDAAAAGLGWFSIGLKGEAVLSIWTYEGVEIVLRSSVIPDRANFFEDAGFTVSKIVSKAD 540

Query: 541 KAANKPLGQSEKKKKGDPKVHVLAKT 567
           +AANK LGQSEKKKKGDPKVH+LAKT
Sbjct: 541 QAANKQLGQSEKKKKGDPKVHMLAKT 566

BLAST of PI0001851 vs. ExPASy TrEMBL
Match: A0A5A7TAR0 (GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold366G00150 PE=4 SV=1)

HSP 1 Score: 1043.1 bits (2696), Expect = 4.2e-301
Identity = 517/559 (92.49%), Postives = 535/559 (95.71%), Query Frame = 0

Query: 1   MLITRKLSASNLKSFLSLRFLSHLSSSPTCSNPPILFPLPFTLSRSFSSKPTPPQASNLS 60
           MLI RKLSASNLKSFLS RF+ HLSSSPTCSNP ILFPLP TL  SFSSKPTPPQA NLS
Sbjct: 1   MLIARKLSASNLKSFLSPRFIYHLSSSPTCSNPSILFPLPSTLFCSFSSKPTPPQALNLS 60

Query: 61  RDGNFDETTSRFSLVCPGCGVHMQDSNPKHPGFFLKPSKKDSNYRLLTHLVPVDDESECS 120
           RDGNFDETTSR S VCPGCGVHMQDSNPKHPGFF+KPSKKDS+YR LTHLVPVDDESECS
Sbjct: 61  RDGNFDETTSRLSHVCPGCGVHMQDSNPKHPGFFIKPSKKDSSYRRLTHLVPVDDESECS 120

Query: 121 DFLKRGLVIEPEKEKTEEDVIEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDFDHTI 180
           +FLKRGLVIEPEKEKTEEDVIEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDF HT+
Sbjct: 121 EFLKRGLVIEPEKEKTEEDVIEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDFKHTL 180

Query: 181 GRRLVSTTGARSVVLIVVDAADFDGSFPKKVANLVSASIEHNSAAWKQGKSGNVPRVVLV 240
           G RLVSTTG RSVVLIVVDAADFDGSFPKKVANLVSASIE+NSAAWKQGKSGNVPRVVLV
Sbjct: 181 GGRLVSTTGTRSVVLIVVDAADFDGSFPKKVANLVSASIENNSAAWKQGKSGNVPRVVLV 240

Query: 241 VTKIDLLPSSLSPTGFEHWVRQRAREGGINKITSLHMVSSIRDWGMKNLVEDVIELAGAR 300
           VTK DLLPSSLSPT FE WVRQRAREGGINKITSLHMVS+I+DWG+KNLVEDVIELAGAR
Sbjct: 241 VTKTDLLPSSLSPTTFERWVRQRAREGGINKITSLHMVSAIKDWGIKNLVEDVIELAGAR 300

Query: 301 GHVWAIGAQNAGKSTLINSIGKHVGGKITQLTEAPVPGTTLGIIRVEGIFPGQAKLFDTP 360
           G+VWAIGAQNAGKSTLINSIGKHVGGK+T LTEAPVPGTTLGIIRVEGIFP QAKLFDTP
Sbjct: 301 GNVWAIGAQNAGKSTLINSIGKHVGGKVTHLTEAPVPGTTLGIIRVEGIFPEQAKLFDTP 360

Query: 361 GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVEETNVDTIYVT 420
           GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDV+ETNVDTIYVT
Sbjct: 361 GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVDETNVDTIYVT 420

Query: 421 VWASPYLPLHMGKTENATKIQEDHFGNHLQPPIGKDRVAELGKWVRREFHVCGTSWDSSC 480
           VWASPYLPLHMGKTENATKI+EDHFG  LQPPIGKDRVAELGKWVR+EF VCGTSWDSSC
Sbjct: 421 VWASPYLPLHMGKTENATKIREDHFGIQLQPPIGKDRVAELGKWVRKEFRVCGTSWDSSC 480

Query: 481 VDAAAAGLGWFAIGLKGEAVLNIWTYEGVEIVLRSSVIPFRSNFFEDAGFTVSKIVSKAD 540
           VDAAAAGLGWF+IGLKGEAVL+IWTYEGVEIVLRSSVIP R+NFFEDAGFTVSKIVSKAD
Sbjct: 481 VDAAAAGLGWFSIGLKGEAVLSIWTYEGVEIVLRSSVIPDRANFFEDAGFTVSKIVSKAD 540

Query: 541 KAANKPLGQSEKKKKGDPK 560
           +AANK LGQSEKKKKGDPK
Sbjct: 541 QAANKQLGQSEKKKKGDPK 559

BLAST of PI0001851 vs. ExPASy TrEMBL
Match: A0A6J1FW06 (GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111448990 PE=4 SV=1)

HSP 1 Score: 990.7 bits (2560), Expect = 2.5e-285
Identity = 492/581 (84.68%), Postives = 531/581 (91.39%), Query Frame = 0

Query: 1   MLITRKLSASNLKSFLSLRFL-----SHLSSSPTCSNPPILFPL---------PFTLSRS 60
           MLI RKLSA  LK  LSLRF+     S L SSP+CSNP IL PL         P + S+ 
Sbjct: 1   MLIARKLSALKLKPILSLRFVSPLQRSQLFSSPSCSNPRILCPLPSATPLPLSPSSSSQP 60

Query: 61  FSSKPTPPQASNLSRDGNFDETTSRFSLVCPGCGVHMQDSNPKHPGFFLKPSKKDSNYRL 120
           FSSKPTPP++  L+RDGN+ E TSRFSLVCPGCGVHMQD+N KHPGFF+KPS+KDSNYRL
Sbjct: 61  FSSKPTPPESPLLTRDGNYSEATSRFSLVCPGCGVHMQDTNRKHPGFFVKPSRKDSNYRL 120

Query: 121 LTHLVPVDDESECSDFLKRGLVIEPEKEKTEEDVIEKPQKPTVCSRCHSLRHYGKVKDPS 180
           LTHLVPVDDESECSD LKRGLV+EPE +K+EE+V EK QKPTVCSRCHSLRHYGKVKDP+
Sbjct: 121 LTHLVPVDDESECSDLLKRGLVMEPEHQKSEENVNEKAQKPTVCSRCHSLRHYGKVKDPA 180

Query: 181 VENLLPDFDFDHTIGRRLVSTTGARSVVLIVVDAADFDGSFPKKVANLVSASIEHNSAAW 240
           +ENLLPDFDFDHTIGRRLVSTTG RSVVL+VVDAADFDGSFPKKVANLVSA+IE NS  W
Sbjct: 181 LENLLPDFDFDHTIGRRLVSTTGTRSVVLLVVDAADFDGSFPKKVANLVSATIEDNSTVW 240

Query: 241 KQGKSGNVPRVVLVVTKIDLLPSSLSPTGFEHWVRQRAREGGINKITSLHMVSSIRDWGM 300
           KQGKSGNVPRVVLVVTKIDLLPSS+SPT FEHWVRQRAREGGINKITSLHMVS++RDWG+
Sbjct: 241 KQGKSGNVPRVVLVVTKIDLLPSSVSPTRFEHWVRQRAREGGINKITSLHMVSAVRDWGL 300

Query: 301 KNLVEDVIELAGARGHVWAIGAQNAGKSTLINSIGKHVGGKITQLTEAPVPGTTLGIIRV 360
           K+LVEDVI+L G RG+VWA+GAQNAGKSTLINSIGKHVGGKITQLTEAPVPGTTLGIIRV
Sbjct: 301 KSLVEDVIDLVGPRGNVWAVGAQNAGKSTLINSIGKHVGGKITQLTEAPVPGTTLGIIRV 360

Query: 361 EGIFPGQAKLFDTPGLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMR 420
           EG+FPGQAKLFDTPGLLNPHQITTRLTREEQKLVHISKELKPRTYRIK GHTIHVAGLMR
Sbjct: 361 EGMFPGQAKLFDTPGLLNPHQITTRLTREEQKLVHISKELKPRTYRIKAGHTIHVAGLMR 420

Query: 421 LDVEETNVDTIYVTVWASPYLPLHMGKTENATKIQEDHFGNHLQPPIGKDRVAELGKWVR 480
           LDVEET+VDTIYVTVWASPYLPLHMGKTENA+KI EDHFGN LQPPIGKDRVAELGKWVR
Sbjct: 421 LDVEETSVDTIYVTVWASPYLPLHMGKTENASKILEDHFGNQLQPPIGKDRVAELGKWVR 480

Query: 481 REFHVCGTSWDSSCVDAAAAGLGWFAIGLKGEAVLNIWTYEGVEIVLRSSVIPFRSNFFE 540
           REFHVCG+SWDSSC+DAAAAGLGWFAIGLKGEAVL+IWTYEG+E+VLRSSVIP RSNFFE
Sbjct: 481 REFHVCGSSWDSSCMDAAAAGLGWFAIGLKGEAVLSIWTYEGIEVVLRSSVIPDRSNFFE 540

Query: 541 DAGFTVSKIVSKADKAANKPLGQSEKK-KKGDPKVHVLAKT 567
           DAGFTVSK+VSKAD+AANK L QSE+K KKGD KVHVLA+T
Sbjct: 541 DAGFTVSKVVSKADQAANKSLDQSERKTKKGDSKVHVLAET 581

BLAST of PI0001851 vs. NCBI nr
Match: XP_004142247.1 (GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic isoform X1 [Cucumis sativus] >KGN54094.1 hypothetical protein Csa_018182 [Cucumis sativus])

HSP 1 Score: 1064.3 bits (2751), Expect = 3.6e-307
Identity = 526/566 (92.93%), Postives = 544/566 (96.11%), Query Frame = 0

Query: 1   MLITRKLSASNLKSFLSLRFLSHLSSSPTCSNPPILFPLPFTLSRSFSSKPTPPQASNLS 60
           MLI RKLSASNLKSFL LRF+SHLSSSP CSNP ILFPLP TLSRSFSSKPT PQA +LS
Sbjct: 1   MLIARKLSASNLKSFLFLRFISHLSSSPICSNPSILFPLPSTLSRSFSSKPTSPQAFSLS 60

Query: 61  RDGNFDETTSRFSLVCPGCGVHMQDSNPKHPGFFLKPSKKDSNYRLLTHLVPVDDESECS 120
           RDGNFDETTSRFSLVCPGCGVHMQDSNPKHPGFF+KPSKKDSNYRLLTHLVPVDDESECS
Sbjct: 61  RDGNFDETTSRFSLVCPGCGVHMQDSNPKHPGFFIKPSKKDSNYRLLTHLVPVDDESECS 120

Query: 121 DFLKRGLVIEPEKEKTEEDVIEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDFDHTI 180
           +FLKRGLVIEPEKEKTEEDV EKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDF+HT+
Sbjct: 121 EFLKRGLVIEPEKEKTEEDVNEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDFNHTL 180

Query: 181 GRRLVSTTGARSVVLIVVDAADFDGSFPKKVANLVSASIEHNSAAWKQGKSGNVPRVVLV 240
           G RLVSTTG RSVVLIVVDAADFDGSFPKKVANLVSASIE+NSAAWKQGKSGNVPRVVLV
Sbjct: 181 GGRLVSTTGTRSVVLIVVDAADFDGSFPKKVANLVSASIENNSAAWKQGKSGNVPRVVLV 240

Query: 241 VTKIDLLPSSLSPTGFEHWVRQRAREGGINKITSLHMVSSIRDWGMKNLVEDVIELAGAR 300
           VTK DLLPSSLSP  FEHWVRQRAREGGINKITSLHMVSSIRDWG+KNLVEDVIELAGAR
Sbjct: 241 VTKTDLLPSSLSPEKFEHWVRQRAREGGINKITSLHMVSSIRDWGIKNLVEDVIELAGAR 300

Query: 301 GHVWAIGAQNAGKSTLINSIGKHVGGKITQLTEAPVPGTTLGIIRVEGIFPGQAKLFDTP 360
           G+VWAIGAQNAGKSTLINSIGKHVGGKITQLTEAPVPGTTLGIIRVEGIFP QAKLFDTP
Sbjct: 301 GNVWAIGAQNAGKSTLINSIGKHVGGKITQLTEAPVPGTTLGIIRVEGIFPAQAKLFDTP 360

Query: 361 GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVEETNVDTIYVT 420
           GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVEETN+DTIYVT
Sbjct: 361 GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVEETNLDTIYVT 420

Query: 421 VWASPYLPLHMGKTENATKIQEDHFGNHLQPPIGKDRVAELGKWVRREFHVCGTSWDSSC 480
           VWASPYLPLHMGKTENATK+QEDHFG  LQPPIGKDRV ELGKWVRREF VCGTSWDSSC
Sbjct: 421 VWASPYLPLHMGKTENATKMQEDHFGIQLQPPIGKDRVEELGKWVRREFRVCGTSWDSSC 480

Query: 481 VDAAAAGLGWFAIGLKGEAVLNIWTYEGVEIVLRSSVIPFRSNFFEDAGFTVSKIVSKAD 540
           VDAA AGLGWFAIGLKGE VL+IWTYEGV+IVLRSSVIP R+NFFEDAGFTVSKIVSKAD
Sbjct: 481 VDAAVAGLGWFAIGLKGEVVLSIWTYEGVDIVLRSSVIPHRANFFEDAGFTVSKIVSKAD 540

Query: 541 KAANKPLGQSEKKKKGDPKVHVLAKT 567
           +A+NKPLGQSEKKKK DP+VHVLA+T
Sbjct: 541 QASNKPLGQSEKKKKSDPEVHVLAQT 566

BLAST of PI0001851 vs. NCBI nr
Match: XP_008449781.1 (PREDICTED: GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic [Cucumis melo])

HSP 1 Score: 1055.0 bits (2727), Expect = 2.2e-304
Identity = 523/566 (92.40%), Postives = 542/566 (95.76%), Query Frame = 0

Query: 1   MLITRKLSASNLKSFLSLRFLSHLSSSPTCSNPPILFPLPFTLSRSFSSKPTPPQASNLS 60
           MLI RKLSASNLKSFLS RF+ HLSSSPTCSNP ILFPLP TL  SFSSKPTPPQA NLS
Sbjct: 1   MLIARKLSASNLKSFLSPRFIYHLSSSPTCSNPSILFPLPSTLFCSFSSKPTPPQALNLS 60

Query: 61  RDGNFDETTSRFSLVCPGCGVHMQDSNPKHPGFFLKPSKKDSNYRLLTHLVPVDDESECS 120
           RDGNFDETTSR S VCPGCGVHMQDSNPKHPGFF+KPSKKDS+YR LTHLVPVDDESECS
Sbjct: 61  RDGNFDETTSRLSHVCPGCGVHMQDSNPKHPGFFIKPSKKDSSYRRLTHLVPVDDESECS 120

Query: 121 DFLKRGLVIEPEKEKTEEDVIEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDFDHTI 180
           +FLKRGLVIEPEKEKTEEDVIEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDF HT+
Sbjct: 121 EFLKRGLVIEPEKEKTEEDVIEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDFKHTL 180

Query: 181 GRRLVSTTGARSVVLIVVDAADFDGSFPKKVANLVSASIEHNSAAWKQGKSGNVPRVVLV 240
           G RLVSTTG RSVVLIVVDAADFDGSFPKKVANLVSASIE+NSAAWKQGKSGNVPRVVLV
Sbjct: 181 GGRLVSTTGTRSVVLIVVDAADFDGSFPKKVANLVSASIENNSAAWKQGKSGNVPRVVLV 240

Query: 241 VTKIDLLPSSLSPTGFEHWVRQRAREGGINKITSLHMVSSIRDWGMKNLVEDVIELAGAR 300
           VTK DLLPSSLSPT FE WVRQRAREGGINKITSLHMVS+I+DWG+KNLVEDVIELAGAR
Sbjct: 241 VTKTDLLPSSLSPTTFERWVRQRAREGGINKITSLHMVSAIKDWGIKNLVEDVIELAGAR 300

Query: 301 GHVWAIGAQNAGKSTLINSIGKHVGGKITQLTEAPVPGTTLGIIRVEGIFPGQAKLFDTP 360
           G+VWAIGAQNAGKSTLINSIGKHVGGK+T LTEAPVPGTTLGIIRVEGIFP QAKLFDTP
Sbjct: 301 GNVWAIGAQNAGKSTLINSIGKHVGGKVTHLTEAPVPGTTLGIIRVEGIFPEQAKLFDTP 360

Query: 361 GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVEETNVDTIYVT 420
           GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDV+ETNVDTIYVT
Sbjct: 361 GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVDETNVDTIYVT 420

Query: 421 VWASPYLPLHMGKTENATKIQEDHFGNHLQPPIGKDRVAELGKWVRREFHVCGTSWDSSC 480
           VWASPYLPLHMGKTENATKI+EDHFG  LQPPIGKDRVAELGKWVR+EF VCGTSWDSSC
Sbjct: 421 VWASPYLPLHMGKTENATKIREDHFGIQLQPPIGKDRVAELGKWVRKEFRVCGTSWDSSC 480

Query: 481 VDAAAAGLGWFAIGLKGEAVLNIWTYEGVEIVLRSSVIPFRSNFFEDAGFTVSKIVSKAD 540
           VDAAAAGLGWF+IGLKGEAVL+IWTYEGVEIVLRSSVIP R+NFFEDAGFTVSKIVSKAD
Sbjct: 481 VDAAAAGLGWFSIGLKGEAVLSIWTYEGVEIVLRSSVIPDRANFFEDAGFTVSKIVSKAD 540

Query: 541 KAANKPLGQSEKKKKGDPKVHVLAKT 567
           +AANK LGQSEKKKKGDPKVH+LAKT
Sbjct: 541 QAANKQLGQSEKKKKGDPKVHMLAKT 566

BLAST of PI0001851 vs. NCBI nr
Match: TYK21671.1 (GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1053.1 bits (2722), Expect = 8.4e-304
Identity = 522/566 (92.23%), Postives = 542/566 (95.76%), Query Frame = 0

Query: 1   MLITRKLSASNLKSFLSLRFLSHLSSSPTCSNPPILFPLPFTLSRSFSSKPTPPQASNLS 60
           MLI RKLSASNLKSFLS RF+ HLSSSPTCSNP ILFPLP TL  SFSSKPTPPQA NLS
Sbjct: 1   MLIARKLSASNLKSFLSPRFIYHLSSSPTCSNPSILFPLPSTLFCSFSSKPTPPQALNLS 60

Query: 61  RDGNFDETTSRFSLVCPGCGVHMQDSNPKHPGFFLKPSKKDSNYRLLTHLVPVDDESECS 120
           RDGNFDETTSR S VCPGCGVHMQDSNPKHPGFF+KPSKKDS+YR LTHLVPV+DESECS
Sbjct: 61  RDGNFDETTSRLSHVCPGCGVHMQDSNPKHPGFFIKPSKKDSSYRRLTHLVPVNDESECS 120

Query: 121 DFLKRGLVIEPEKEKTEEDVIEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDFDHTI 180
           +FLKRGLVIEPEKEKTEEDVIEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDF HT+
Sbjct: 121 EFLKRGLVIEPEKEKTEEDVIEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDFKHTL 180

Query: 181 GRRLVSTTGARSVVLIVVDAADFDGSFPKKVANLVSASIEHNSAAWKQGKSGNVPRVVLV 240
           G RLVSTTG RSVVLIVVDAADFDGSFPKKVANLVSASIE+NSAAWKQGKSGNVPRVVLV
Sbjct: 181 GGRLVSTTGTRSVVLIVVDAADFDGSFPKKVANLVSASIENNSAAWKQGKSGNVPRVVLV 240

Query: 241 VTKIDLLPSSLSPTGFEHWVRQRAREGGINKITSLHMVSSIRDWGMKNLVEDVIELAGAR 300
           VTK DLLPSSLSPT FE WVRQRAREGGINKITSLHMVS+I+DWG+KNLVEDVIELAGAR
Sbjct: 241 VTKTDLLPSSLSPTTFERWVRQRAREGGINKITSLHMVSAIKDWGIKNLVEDVIELAGAR 300

Query: 301 GHVWAIGAQNAGKSTLINSIGKHVGGKITQLTEAPVPGTTLGIIRVEGIFPGQAKLFDTP 360
           G+VWAIGAQNAGKSTLINSIGKHVGGK+T LTEAPVPGTTLGIIRVEGIFP QAKLFDTP
Sbjct: 301 GNVWAIGAQNAGKSTLINSIGKHVGGKVTHLTEAPVPGTTLGIIRVEGIFPEQAKLFDTP 360

Query: 361 GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVEETNVDTIYVT 420
           GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDV+ETNVDTIYVT
Sbjct: 361 GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVDETNVDTIYVT 420

Query: 421 VWASPYLPLHMGKTENATKIQEDHFGNHLQPPIGKDRVAELGKWVRREFHVCGTSWDSSC 480
           VWASPYLPLHMGKTENATKI+EDHFG  LQPPIGKDRVAELGKWVR+EF VCGTSWDSSC
Sbjct: 421 VWASPYLPLHMGKTENATKIREDHFGIQLQPPIGKDRVAELGKWVRKEFRVCGTSWDSSC 480

Query: 481 VDAAAAGLGWFAIGLKGEAVLNIWTYEGVEIVLRSSVIPFRSNFFEDAGFTVSKIVSKAD 540
           VDAAAAGLGWF+IGLKGEAVL+IWTYEGVEIVLRSSVIP R+NFFEDAGFTVSKIVSKAD
Sbjct: 481 VDAAAAGLGWFSIGLKGEAVLSIWTYEGVEIVLRSSVIPDRANFFEDAGFTVSKIVSKAD 540

Query: 541 KAANKPLGQSEKKKKGDPKVHVLAKT 567
           +AANK LGQSEKKKKGDPKVH+LAKT
Sbjct: 541 QAANKQLGQSEKKKKGDPKVHMLAKT 566

BLAST of PI0001851 vs. NCBI nr
Match: KAA0040048.1 (GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1043.1 bits (2696), Expect = 8.7e-301
Identity = 517/559 (92.49%), Postives = 535/559 (95.71%), Query Frame = 0

Query: 1   MLITRKLSASNLKSFLSLRFLSHLSSSPTCSNPPILFPLPFTLSRSFSSKPTPPQASNLS 60
           MLI RKLSASNLKSFLS RF+ HLSSSPTCSNP ILFPLP TL  SFSSKPTPPQA NLS
Sbjct: 1   MLIARKLSASNLKSFLSPRFIYHLSSSPTCSNPSILFPLPSTLFCSFSSKPTPPQALNLS 60

Query: 61  RDGNFDETTSRFSLVCPGCGVHMQDSNPKHPGFFLKPSKKDSNYRLLTHLVPVDDESECS 120
           RDGNFDETTSR S VCPGCGVHMQDSNPKHPGFF+KPSKKDS+YR LTHLVPVDDESECS
Sbjct: 61  RDGNFDETTSRLSHVCPGCGVHMQDSNPKHPGFFIKPSKKDSSYRRLTHLVPVDDESECS 120

Query: 121 DFLKRGLVIEPEKEKTEEDVIEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDFDHTI 180
           +FLKRGLVIEPEKEKTEEDVIEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDF HT+
Sbjct: 121 EFLKRGLVIEPEKEKTEEDVIEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFDFKHTL 180

Query: 181 GRRLVSTTGARSVVLIVVDAADFDGSFPKKVANLVSASIEHNSAAWKQGKSGNVPRVVLV 240
           G RLVSTTG RSVVLIVVDAADFDGSFPKKVANLVSASIE+NSAAWKQGKSGNVPRVVLV
Sbjct: 181 GGRLVSTTGTRSVVLIVVDAADFDGSFPKKVANLVSASIENNSAAWKQGKSGNVPRVVLV 240

Query: 241 VTKIDLLPSSLSPTGFEHWVRQRAREGGINKITSLHMVSSIRDWGMKNLVEDVIELAGAR 300
           VTK DLLPSSLSPT FE WVRQRAREGGINKITSLHMVS+I+DWG+KNLVEDVIELAGAR
Sbjct: 241 VTKTDLLPSSLSPTTFERWVRQRAREGGINKITSLHMVSAIKDWGIKNLVEDVIELAGAR 300

Query: 301 GHVWAIGAQNAGKSTLINSIGKHVGGKITQLTEAPVPGTTLGIIRVEGIFPGQAKLFDTP 360
           G+VWAIGAQNAGKSTLINSIGKHVGGK+T LTEAPVPGTTLGIIRVEGIFP QAKLFDTP
Sbjct: 301 GNVWAIGAQNAGKSTLINSIGKHVGGKVTHLTEAPVPGTTLGIIRVEGIFPEQAKLFDTP 360

Query: 361 GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVEETNVDTIYVT 420
           GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDV+ETNVDTIYVT
Sbjct: 361 GLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVDETNVDTIYVT 420

Query: 421 VWASPYLPLHMGKTENATKIQEDHFGNHLQPPIGKDRVAELGKWVRREFHVCGTSWDSSC 480
           VWASPYLPLHMGKTENATKI+EDHFG  LQPPIGKDRVAELGKWVR+EF VCGTSWDSSC
Sbjct: 421 VWASPYLPLHMGKTENATKIREDHFGIQLQPPIGKDRVAELGKWVRKEFRVCGTSWDSSC 480

Query: 481 VDAAAAGLGWFAIGLKGEAVLNIWTYEGVEIVLRSSVIPFRSNFFEDAGFTVSKIVSKAD 540
           VDAAAAGLGWF+IGLKGEAVL+IWTYEGVEIVLRSSVIP R+NFFEDAGFTVSKIVSKAD
Sbjct: 481 VDAAAAGLGWFSIGLKGEAVLSIWTYEGVEIVLRSSVIPDRANFFEDAGFTVSKIVSKAD 540

Query: 541 KAANKPLGQSEKKKKGDPK 560
           +AANK LGQSEKKKKGDPK
Sbjct: 541 QAANKQLGQSEKKKKGDPK 559

BLAST of PI0001851 vs. NCBI nr
Match: XP_038901740.1 (GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic [Benincasa hispida])

HSP 1 Score: 1040.8 bits (2690), Expect = 4.3e-300
Identity = 511/571 (89.49%), Postives = 543/571 (95.10%), Query Frame = 0

Query: 1   MLITRKLSASNLKSFLSLRFL-----SHLSSSPTCSNPPILFPLPFTLSRSFSSKPTPPQ 60
           MLITRKLSASNLKSFLSLR +     S L SSP C NPPILFP P TL RSFSSKPTPP+
Sbjct: 1   MLITRKLSASNLKSFLSLRSVSLSLSSQLFSSPLCLNPPILFPHPSTLPRSFSSKPTPPE 60

Query: 61  ASNLSRDGNFDETTSRFSLVCPGCGVHMQDSNPKHPGFFLKPSKKDSNYRLLTHLVPVDD 120
           ASNL+RDGN+DE TSRFSLVCPGCGVHMQD+NPKHPGFF+KPS+KD NYRLLTHLVPVDD
Sbjct: 61  ASNLTRDGNYDEATSRFSLVCPGCGVHMQDTNPKHPGFFVKPSRKDPNYRLLTHLVPVDD 120

Query: 121 ESECSDFLKRGLVIEPEKEKTEEDVIEKPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFD 180
           ESECS+FLKRGLVI+PE +KTEE+VIE+PQKPTVCSRCHSLRHYGKVKDPSVENLLPDFD
Sbjct: 121 ESECSEFLKRGLVIDPENQKTEENVIEQPQKPTVCSRCHSLRHYGKVKDPSVENLLPDFD 180

Query: 181 FDHTIGRRLVSTTGARSVVLIVVDAADFDGSFPKKVANLVSASIEHNSAAWKQGKSGNVP 240
           FDHTIGRRLVSTTG RSVV+IVVDA DFDGSFPKKVANLVSA+IE NSAAWKQGKSGNVP
Sbjct: 181 FDHTIGRRLVSTTGTRSVVMIVVDATDFDGSFPKKVANLVSATIEDNSAAWKQGKSGNVP 240

Query: 241 RVVLVVTKIDLLPSSLSPTGFEHWVRQRAREGGINKITSLHMVSSIRDWGMKNLVEDVIE 300
           RVVLVVTKIDLLPSS+SPT FEHWVRQRAREGGINKITSLHMVS++RDWG+KNLVEDVI+
Sbjct: 241 RVVLVVTKIDLLPSSISPTRFEHWVRQRAREGGINKITSLHMVSAVRDWGLKNLVEDVID 300

Query: 301 LAGARGHVWAIGAQNAGKSTLINSIGKHVGGKITQLTEAPVPGTTLGIIRVEGIFPGQAK 360
           L GARG+VWAIGAQNAGKSTLINSIGKHVGGKITQLTEAPVPGTTLGIIR+EGIFPGQA+
Sbjct: 301 LVGARGNVWAIGAQNAGKSTLINSIGKHVGGKITQLTEAPVPGTTLGIIRMEGIFPGQAR 360

Query: 361 LFDTPGLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVEETNVD 420
           LFDTPGLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVEET+VD
Sbjct: 361 LFDTPGLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVEETSVD 420

Query: 421 TIYVTVWASPYLPLHMGKTENATKIQEDHFGNHLQPPIGKDRVAELGKWVRREFHVCGTS 480
           TIYVTVWASPYLPLHMGKTENA+KIQ DHFGN LQPPIGKDRVAELGKWVRREF VCG+S
Sbjct: 421 TIYVTVWASPYLPLHMGKTENASKIQ-DHFGNQLQPPIGKDRVAELGKWVRREFRVCGSS 480

Query: 481 WDSSCVDAAAAGLGWFAIGLKGEAVLNIWTYEGVEIVLRSSVIPFRSNFFEDAGFTVSKI 540
           WDSSCVDAAAAGLGWFAIGLKGEAVL+IWTYEGVE+V+RSSVIP+RSNFFEDAGFT+SKI
Sbjct: 481 WDSSCVDAAAAGLGWFAIGLKGEAVLSIWTYEGVEVVIRSSVIPYRSNFFEDAGFTISKI 540

Query: 541 VSKADKAANKPLGQSEKKKKGDPKVHVLAKT 567
           VSKAD+ A KPL Q EKKKKGDPKVHVLA+T
Sbjct: 541 VSKADQVATKPLDQREKKKKGDPKVHVLAET 570

BLAST of PI0001851 vs. TAIR 10
Match: AT4G10620.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 732.3 bits (1889), Expect = 3.1e-211
Identity = 362/568 (63.73%), Postives = 444/568 (78.17%), Query Frame = 0

Query: 5   RKLSASNLKSFLSLRFLSHLSSSPTCSNPPILFPLPFTLSRSFSSKPTPP--------QA 64
           R+LS+S LK   +L   S  SS PT  NP    P  +     F++   PP         +
Sbjct: 7   RELSSSKLKPLFALHLSSFKSSIPTKPNPS---PPSYLNPHHFNNISKPPFLRFYSSSSS 66

Query: 65  SN---LSRDGNFDETTSRFSLVCPGCGVHMQDSNPKHPGFFLKPS-KKDSNYRLLTHLVP 124
           SN   L+RDGN+++TTS    VCPGCGVHMQ+SNPKHPGFF+KPS +K  N   L  L P
Sbjct: 67  SNLLPLNRDGNYNDTTSITISVCPGCGVHMQNSNPKHPGFFIKPSTEKQRNDLNLRDLTP 126

Query: 125 VDDESECSDFLKRGLVIEP--EKEKTEEDVIEKPQKPTVCSRCHSLRHYGKVKDPSVENL 184
           +  E E  D +KRG +IEP    +    D      +P VC+RCHSLRHYG+VKDP+VENL
Sbjct: 127 ISQEPEFIDSIKRGFIIEPISSSDLNPRDDEPSDSRPLVCARCHSLRHYGRVKDPTVENL 186

Query: 185 LPDFDFDHTIGRRLVSTTGARSVVLIVVDAADFDGSFPKKVANLVSASIEHNSAAWKQGK 244
           LPDFDFDHT+GRRL S +GAR+VVL+VVDA+DFDGSFPK+VA LVS +I+ N+ AWK+GK
Sbjct: 187 LPDFDFDHTVGRRLGSASGARTVVLMVVDASDFDGSFPKRVAKLVSRTIDENNMAWKEGK 246

Query: 245 SGNVPRVVLVVTKIDLLPSSLSPTGFEHWVRQRAREGGINKITSLHMVSSIRDWGMKNLV 304
           SGNVPRVV+VVTKIDLLPSSLSP  FE WVR RAREGG++KIT LH VS +++WG+K+LV
Sbjct: 247 SGNVPRVVVVVTKIDLLPSSLSPNRFEQWVRLRAREGGLSKITKLHFVSPVKNWGIKDLV 306

Query: 305 EDVIELAGARGHVWAIGAQNAGKSTLINSIGKHVGGKITQLTEAPVPGTTLGIIRVEGIF 364
           EDV  +AG RGHVWA+G+QNAGKSTLIN++GK VGGK+  LTEAPVPGTTLGIIR+EG+ 
Sbjct: 307 EDVAAMAGKRGHVWAVGSQNAGKSTLINAVGKVVGGKVWHLTEAPVPGTTLGIIRIEGVL 366

Query: 365 PGQAKLFDTPGLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIHVAGLMRLDVE 424
           P +AKLFDTPGLLNPHQITTRLTREEQ+LVHISKELKPRTYRIK G+T+H+ GLMRLD++
Sbjct: 367 PFEAKLFDTPGLLNPHQITTRLTREEQRLVHISKELKPRTYRIKEGYTVHIGGLMRLDID 426

Query: 425 ETNVDTIYVTVWASPYLPLHMGKTENATKIQEDHFGNHLQPPIGKDRVAELGKWVRREFH 484
           E +VD++YVTVWASPY+PLHMGK ENA K  EDHFG  LQPPIG+ RV ELGKWVR+EF 
Sbjct: 427 EASVDSLYVTVWASPYVPLHMGKKENAYKTLEDHFGCRLQPPIGEKRVEELGKWVRKEFR 486

Query: 485 VCGTSWDSSCVDAAAAGLGWFAIGLKGEAVLNIWTYEGVEIVLRSSVIPFRSNFFEDAGF 544
           V GTSWD+S VD A +GLGWFA+GLKG+A+L +WT+EG+++  R S++P R++ FED+GF
Sbjct: 487 VSGTSWDTSSVDIAVSGLGWFALGLKGDAILGVWTHEGIDVFCRDSLLPQRAHTFEDSGF 546

Query: 545 TVSKIVSKADKAANKPLGQSEKKKKGDP 559
           TVSKIV+KAD+  N+ + + E +KK  P
Sbjct: 547 TVSKIVAKADRNFNQ-IHKEETQKKRKP 570

BLAST of PI0001851 vs. TAIR 10
Match: AT3G57180.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 448.7 bits (1153), Expect = 6.7e-126
Identity = 235/575 (40.87%), Postives = 344/575 (59.83%), Query Frame = 0

Query: 53  PPQASNLSRDGNFDETTSRFSLVCPGCGVHMQDSNPKHPGFFLKPSKKDSNYRLLTHL-- 112
           P ++ NL      DE      ++CPGCG+ MQD++P  PG++ K     +N     H+  
Sbjct: 75  PKKSKNLIMSEGRDEDEDYGKIICPGCGIFMQDNDPDLPGYYQKRKVIANNLEGDEHVEN 134

Query: 113 ------VPVDDES---------ECSDFLKRGLV--------------------------- 172
                   VDD++         E  D +K  +                            
Sbjct: 135 DELAGFEMVDDDADEEEEGEDDEMDDEIKNAIEGSNSESESGFEWESDEWEEKKEVNDVE 194

Query: 173 -------------IEPEKE------KTEEDVIEKPQKP-------TVCSRCHSLRHYGKV 232
                        +  EKE      KTE   I + +         TVC+RCHSLR+YG+V
Sbjct: 195 LDGFAPAGVGYGNVTEEKEKKKRVSKTERKKIAREEAKKDNYDDVTVCARCHSLRNYGQV 254

Query: 233 KDPSVENLLPDFDFDHTIGRRLVS--TTGARSVVLIVVDAADFDGSFPKKVANLVSASIE 292
           K+ + ENLLPDFDFD  I  RL+   +  + +VV++VVD  DFDGSFPK+ A  +   ++
Sbjct: 255 KNQAAENLLPDFDFDRLISTRLIKPMSNSSTTVVVMVVDCVDFDGSFPKRAAKSLFQVLQ 314

Query: 293 HNSAAWKQGKSGNVPRVVLVVTKIDLLPSSLSPTGFEHWVRQRAREGGINKITSLHMVSS 352
                 K  K  N+P++VLV TK+DLLP+ +SP   + WVR RA+ GG  K++ ++MVS+
Sbjct: 315 KAENDPKGSK--NLPKLVLVATKVDLLPTQISPARLDRWVRHRAKAGGAPKLSGVYMVSA 374

Query: 353 IRDWGMKNLVEDVIELAGARGHVWAIGAQNAGKSTLINSIGKHVGGKITQLTEAPVPGTT 412
            +D G+KNL+  + ELAG RG+VW IGAQNAGKSTLIN++ K  G K+T+LTEAPVPGTT
Sbjct: 375 RKDIGVKNLLAYIKELAGPRGNVWVIGAQNAGKSTLINALSKKDGAKVTRLTEAPVPGTT 434

Query: 413 LGIIRVEGIFPGQAKLFDTPGLLNPHQITTRLTREEQKLVHISKELKPRTYRIKVGHTIH 472
           LGI+++ GI   +AK++DTPGLL+P+ ++ RL  EE+K+V I KE++PR+YR+K G ++H
Sbjct: 435 LGILKIGGILSAKAKMYDTPGLLHPYLMSLRLNSEERKMVEIRKEVQPRSYRVKAGQSVH 494

Query: 473 VAGLMRLDVEETNVDTIYVTVWASPYLPLHMGKTENATKIQEDHFGNHLQPPIGKDRVAE 532
           + GL+RLD+   +V+TIY+T+WAS  + LH+GKTENA +I + H G  LQPPIG++R +E
Sbjct: 495 IGGLVRLDLVSASVETIYITIWASHSVSLHLGKTENAEEIFKGHSGLRLQPPIGENRASE 554

Query: 533 LGKWVRREFHVCGTSWDSSCVDAAAAGLGWFAIGLKGEAVLNIWTYEGVEIVLRSSVIPF 556
           LG W  +E  V G SWD   +D + AGLGW ++GLKG A L +WTY+G+++ LR  ++  
Sbjct: 555 LGTWEEKEIQVSGNSWDVKSIDISVAGLGWLSLGLKGAATLALWTYQGIDVTLREPLVID 614

BLAST of PI0001851 vs. TAIR 10
Match: AT3G47450.2 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 117.5 bits (293), Expect = 3.6e-26
Identity = 96/372 (25.81%), Postives = 161/372 (43.28%), Query Frame = 0

Query: 149 VCSRCHSLRHYGKVKDPSVENLLP---DFDFDHTIGRRLVSTTGARSVVLIVVDAADFDG 208
           +C RC  L H   +         P    F     +  +L      +++++ +VD  DF+G
Sbjct: 144 ICGRCQLLSHGHMITAVGGNGGYPGGKQFVSADELREKLSHLRHEKALIVKLVDIVDFNG 203

Query: 209 SFPKKVANLVSASIEHNSAAWKQGKSGNVPRVVLVVTKIDLLPSSLSPTGFEHWVRQRAR 268
           SF  +V +LV A+                  ++LV+TKIDLLP          WV +   
Sbjct: 204 SFLARVRDLVGAN-----------------PIILVITKIDLLPKGTDMNCIGDWVVEVTM 263

Query: 269 EGGINKITSLHMVSSIRDWGMKNLVEDVIELAGARGHVWAIGAQNAGKSTLINSIGKHV- 328
              +N + S+H+ SS    G+  +  ++ +    R  V+ +GA N GKS  IN++ K + 
Sbjct: 264 RKKLN-VLSVHLTSSKSLDGVSGVASEIQKEKKGR-DVYILGAANVGKSAFINALLKTMA 323

Query: 329 -------GGKITQLTEAPVPGTTLGIIRVEGIFPGQAKLFDTPGLLNPHQITTRLTREEQ 388
                    +  +  ++ VPGTTLG I++   F G  KL+DTPG+   H+    +  ++ 
Sbjct: 324 ERDPVAAAAQKYKPIQSAVPGTTLGPIQINA-FVGGEKLYDTPGVHLHHRQAAVVHSDDL 383

Query: 389 KLVHISKELKPRTYRIKV---------------GHTIHVAGLMRLDVEETNVDTIYVTVW 448
             +     L+ +++ I                 G+T    GL+R+D+ +   +T + T +
Sbjct: 384 PALAPQNRLRGQSFDISTLPTQSSSSPKGESLNGYTFFWGGLVRIDILKALPETCF-TFY 443

Query: 449 ASPYLPLHMGKTENATKIQEDHFGNHLQPPIGKDRVAE-LGKWVRREFHVCGTSWDSSCV 494
               L +H   T+ AT   E   G  L PP GK+++ E  G    R   +          
Sbjct: 444 GPKALEIHAVPTKTATAFYEKELGVLLTPPSGKNQMQEWKGLQSHRLLQIEINDAKRPAS 494

BLAST of PI0001851 vs. TAIR 10
Match: AT3G47450.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 116.7 bits (291), Expect = 6.1e-26
Identity = 96/372 (25.81%), Postives = 161/372 (43.28%), Query Frame = 0

Query: 149 VCSRCHSLRHYGKVKDPSVENLLP---DFDFDHTIGRRLVSTTGARSVVLIVVDAADFDG 208
           +C RC  L H   +         P    F     +  +L      +++++ +VD  DF+G
Sbjct: 144 ICGRCQLLSHGHMITAVGGNGGYPGGKQFVSADELREKLSHLRHEKALIVKLVDIVDFNG 203

Query: 209 SFPKKVANLVSASIEHNSAAWKQGKSGNVPRVVLVVTKIDLLPSSLSPTGFEHWVRQRAR 268
           SF  +V +LV A+                  ++LV+TKIDLLP          WV +   
Sbjct: 204 SFLARVRDLVGAN-----------------PIILVITKIDLLPKGTDMNCIGDWVVEVTM 263

Query: 269 EGGINKITSLHMVSSIRDWGMKNLVEDVIELAGARGHVWAIGAQNAGKSTLINSIGKHV- 328
              +N + S+H+ SS    G+  +  ++ +    R  V+ +GA N GKS  IN++ K + 
Sbjct: 264 RKKLN-VLSVHLTSSKSLDGVSGVASEIQKEKKGR-DVYILGAANVGKSAFINALLKTMA 323

Query: 329 -------GGKITQLTEAPVPGTTLGIIRVEGIFPGQAKLFDTPGLLNPHQITTRLTREEQ 388
                    +  +  ++ VPGTTLG I++   F G  KL+DTPG+   H+    +  ++ 
Sbjct: 324 ERDPVAAAAQKYKPIQSAVPGTTLGPIQINA-FVGGEKLYDTPGVHLHHRQAAVVHSDDL 383

Query: 389 KLVHISKELKPRTYRIKV---------------GHTIHVAGLMRLDVEETNVDTIYVTVW 448
             +     L+ +++ I                 G+T    GL+R+D+ +   +T + T +
Sbjct: 384 PALAPQNRLRGQSFDISTLPTQSSSSPKGESLNGYTFFWGGLVRIDILKALPETCF-TFY 443

Query: 449 ASPYLPLHMGKTENATKIQEDHFGNHLQPPIGKDRVAE-LGKWVRREFHVCGTSWDSSCV 494
               L +H   T+ AT   E   G  L PP GK+++ E  G    R   +          
Sbjct: 444 GPKALEIHAVPTKTATAFYEAKLGVLLTPPSGKNQMQEWKGLQSHRLLQIEINDAKRPAS 494

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8W4I69.5e-12540.87GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic OS=Arab... [more]
P544533.6e-3931.36Uncharacterized protein YqeH OS=Bacillus subtilis (strain 168) OX=224308 GN=yqeH... [more]
Q66GP95.0e-2525.81NO-associated protein 1, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=... [more]
Q6YPG54.7e-2324.86Putative nitric oxide synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g... [more]
Q9JJG94.4e-1328.65Nitric oxide-associated protein 1 OS=Mus musculus OX=10090 GN=Noa1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KZ581.8e-30792.93G domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G285700 PE=4 SV=1[more]
A0A1S3BNH11.1e-30492.40GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic OS=Cucu... [more]
A0A5D3DDC54.1e-30492.23GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2 OS=Cucumis melo var. m... [more]
A0A5A7TAR04.2e-30192.49GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2 OS=Cucumis melo var. m... [more]
A0A6J1FW062.5e-28584.68GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic OS=Cucu... [more]
Match NameE-valueIdentityDescription
XP_004142247.13.6e-30792.93GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic isoform... [more]
XP_008449781.12.2e-30492.40PREDICTED: GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplas... [more]
TYK21671.18.4e-30492.23GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2 [Cucumis melo var. mak... [more]
KAA0040048.18.7e-30192.49GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2 [Cucumis melo var. mak... [more]
XP_038901740.14.3e-30089.49GTP-binding protein BRASSINAZOLE INSENSITIVE PALE GREEN 2, chloroplastic [Beninc... [more]
Match NameE-valueIdentityDescription
AT4G10620.13.1e-21163.73P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT3G57180.16.7e-12640.87P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT3G47450.23.6e-2625.81P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT3G47450.16.1e-2625.81P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006073GTP binding domainPFAMPF01926MMR_HSR1coord: 303..378
e-value: 6.0E-9
score: 36.0
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 182..367
e-value: 6.2E-33
score: 116.0
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 192..381
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 543..566
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 545..566
NoneNo IPR availablePANTHERPTHR46434:SF3GTP-BINDING PROTEIN BRASSINAZOLE INSENSITIVE PALE GREEN 2, CHLOROPLASTICcoord: 52..118
NoneNo IPR availablePANTHERPTHR46434GENETIC INTERACTOR OF PROHIBITINS 3, MITOCHONDRIALcoord: 128..556
coord: 52..118
NoneNo IPR availablePANTHERPTHR46434:SF3GTP-BINDING PROTEIN BRASSINAZOLE INSENSITIVE PALE GREEN 2, CHLOROPLASTICcoord: 128..556
NoneNo IPR availableCDDcd01855YqeHcoord: 150..362
e-value: 1.58724E-60
score: 196.717

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0001851.1PI0001851.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005739 mitochondrion
molecular_function GO:0005525 GTP binding
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0004517 nitric-oxide synthase activity