PI0001755 (gene) Melon (PI 482460) v1

Overview
NamePI0001755
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionCytochrome P450
Locationchr08: 7652436 .. 7655189 (-)
RNA-Seq ExpressionPI0001755
SyntenyPI0001755
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCTTAGCCATCGTAATTCTTGCTCTGCTTTTACTCCTTTGTTTTTCCGCTGCTTTGCTCCGATGGAATGAAGTTCGATACAGAAAAAAACGGTTGCCTCCTGGTACAATGGGTTGGCCTGTTTTTGGTGAAACCTCTGAGTTTCTTAAACAAGGTCCAAATTTTATGAATTCTAAAAGAGCAAGGTACTAATTAAAATTTACTCTGTTTTTCTTTTCTTTTTTTCCTTTTTGCCTTTTGATTTCTGGGTAATCTCTGGTTTTAACGCTTGCTCTGTTTTTTTGTAAAGATATGGGAGCTTTTTCAAATCACATATTTTGGGATCTCCTACAGTTGTTTCTATGGATCCAGAGGTTAATAGATATATTTTAATGAATGAATCTAAAGGACTTGTCGCTGGTTATCCTCAATCTATGCTTGATATACTTGGAAAATGCAATATCGCTGCCGTACACGGTGACGCTCATAAGCTTATGAGAGGTGCTCTACTTGCTATTGTTAGTCCCCCATGATTAAAGATCGACTTCTTCTTCAAGTTGATGAGTTTATGCGATCCCATTTGCGTAATTGGGATAGTCAAATCATTGATATTCAAGAAAAAACTAAACAGGTGGATTTTAGTTTTTAAAAAATTGCTCATTTTTCTCTGTTTTCTCACATTATTAACCTCCCTCCTCCCCCCCCTCTTCAGATGGCTTTCTGGACATCTTTGAAGCAGACTACCAGTATCGAATCCGGTCCATTATGGGAATCTTTCACCATAGAATTCTTTAAGCTCGTCCTGGGCACTTTCTCGCTCCCTATCGATCTTCCCGGCACGAATTACCGTATAGGAGTTCAGGTTAAAACTTTAATTCTAATATGAGATCAAAGCCTGTTAACGTCATTGGAAGAATTTAATTGATAAATTATGGGGTTTTTTTTATGTATTTTTCAGGCAAGGAATAACATAGTAAAGCTATTGAAGCAGCTGATTAAGGAGAGAGGAAATTCAAAGATTCAAAAAGATGACATGCTTGGATATTTATTGAGTGAAGAGAACAAATATAAACTAAATGAGGAACAGATTATTGATCAAGTCATTACTGTTTTCTATTCTGGGTATGAAACTGTTTCAACAACTTCAATGATGGCTGTAAAGTTCCTTCATGATCATCCCAAAGTTCTTCAGCAACTCAGAGTGAGCATTAAAATAAGAAAAAATAAATTTACTACTCAGTGAGTTGGAAATTTGAAAAAGCTATTGGTTTTTTTTTTTTTTTTTTTTTTTCAATTTTTTCTTCTAAGTTAGAGATTTGAAATTTGCAGGAAGAACATTTAGCAATAAGAGAGAAGAAAAATCCTGAGGACCCCATTGATTGGGATGATTTTAAAGCAATGGAGTTCACTCGTGCGGTATGATTTTTTATGTTTCTACTGTCGTTTTTTTAACAGTTCTTCGATGGGTTTTTCTTAAAAAATGATCAAGATTCTAACTTTACACTGTTTTTGTTTTGGAATTGTTTAGGTGATTTTTGAAACATCAAGGCTAGCTACTGTTGTTAATGGAGTTTTGAGGAAAACTACAAAGGATTTGGAATTAAATGGTGAGTTTTATTAATATTTATTTATCTATTTTTTTTTTTGTATAAAATTTGGAGTTTTCTTATGTACTTGTTTAAGATTAGAAAAATCATTCCTTCAAGAGATTCAAATTTTCATTATGCGCCACCTACCACGTCAATGTCATTTCATAAAATTTCTACTTCAAGAGTATTTTGGTAACAAATATAAACGTTGAAAGGATAATGCTTCAAGGGTAATTTTAGTATGTATATAAAAGTTGGGTAATATTGCAAACTTTTAAAAGTATAAGGGCATTTTTAAAACAAGCCGCAAGTTTTGTTTATGAGCTATTTGCAGTTACCAATATTCATCTCCCCAATTCACTTTGGGCCTCTTTTTCTCTCTCTCTCTCCTCTGTTTTTTGTGGATCAGTCTTATATATAAGGATCATAAAGAATGACAGAGCTTCTTCACGTCTTTCATTACAACAATGTGCTGAGATCAAACTAAAAAGTTTCAATCTTTAACTTTTTTTTTTTTTTTTTTACTTTTCTTTTTACAGGGTTTCAAATTCCTAAAGGATGGAGGATTTATGTGTATACTAGAGAGATAAACTATGACCCTTTTCTCTATCCTGAACCATTTACCTTCAACCCTTGGAGATGGCTGGTGAGTCTCTCTCTCTCTCTCTTAACTTTCTTTCTCTCTCTTACCCTTTGTTGAAAATGACCATTTTGCTCTTGTGTAACATTTTTCAGGACAAAAACTTAGAGTCCCATAATTATTTTTTCCTGTTTGGAGGCGGAACTAGACTTTGTCCTGGCAAAGAATTGGGAATTGCAGAGGTTTCTACTTTTTTACATTACTTTGTAACTAAATACAGGTATGTAGCTTTCTTTTTCATATTAGTGTATTCTTAGTACTTAAGTGCTATCCTGATAGGCACACATTTTCGTCCATGCGACAAATTATGATTATATATTTTGTTAGATATTGCATAAAGATAGGTACGGTTCCACATGCAATTATGTATTATTTTTTGCTAATAAAAATTAAAACAGAAATTCTGATAAAGATTTTTTTTTTTTTTTTTTTATTTTTATTTTTTATTTTTTCAGGTGGGAAGAAGTTGGAGGATATAAGCTCTTGAAGTTTCCAAGAGTGAAAGCTCCAAATGGGTTTCACATTAGGGTTTCATCCTATTGA

mRNA sequence

ATGGCCTTAGCCATCGTAATTCTTGCTCTGCTTTTACTCCTTTGTTTTTCCGCTGCTTTGCTCCGATGGAATGAAGTTCGATACAGAAAAAAACGGTTGCCTCCTGGTACAATGGGTTGGCCTGTTTTTGGTGAAACCTCTGAGTTTCTTAAACAAGGTCCAAATTTTATGAATTCTAAAAGAGCAAGATATGGGAGCTTTTTCAAATCACATATTTTGGGATCTCCTACAGTTGTTTCTATGGATCCAGAGGTTAATAGATATATTTTAATGAATGAATCTAAAGGACTTGTCGCTGGTTATCCTCAATCTATGCTTGATATACTTGGAAAATGCAATATCGCTGCCGTACACGGTGACGCTCATAAGCTTATGAGAGGTGCTCTACTTGCTATTGTTATTGATGAGTTTATGCGATCCCATTTGCGTAATTGGGATAGTCAAATCATTGATATTCAAGAAAAAACTAAACAGATGGCTTTCTGGACATCTTTGAAGCAGACTACCAGTATCGAATCCGGTCCATTATGGGAATCTTTCACCATAGAATTCTTTAAGCTCGTCCTGGGCACTTTCTCGCTCCCTATCGATCTTCCCGGCACGAATTACCGTATAGGAGTTCAGGCAAGGAATAACATAGTAAAGCTATTGAAGCAGCTGATTAAGGAGAGAGGAAATTCAAAGATTCAAAAAGATGACATGCTTGGATATTTATTGAGTGAAGAGAACAAATATAAACTAAATGAGGAACAGATTATTGATCAAGTCATTACTGTTTTCTATTCTGGGTATGAAACTGTTTCAACAACTTCAATGATGGCTGTAAAGTTCCTTCATGATCATCCCAAAGTTCTTCAGCAACTCAGAGAAGAACATTTAGCAATAAGAGAGAAGAAAAATCCTGAGGACCCCATTGATTGGGATGATTTTAAAGCAATGGAGTTCACTCGTGCGGTGATTTTTGAAACATCAAGGCTAGCTACTGTTGTTAATGGAGTTTTGAGGAAAACTACAAAGGATTTGGAATTAAATGGGTTTCAAATTCCTAAAGGATGGAGGATTTATGTGTATACTAGAGAGATAAACTATGACCCTTTTCTCTATCCTGAACCATTTACCTTCAACCCTTGGAGATGGCTGGACAAAAACTTAGAGTCCCATAATTATTTTTTCCTGTTTGGAGGCGGAACTAGACTTTGTCCTGGCAAAGAATTGGGAATTGCAGAGGTTTCTACTTTTTTACATTACTTTGTAACTAAATACAGGTGGGAAGAAGTTGGAGGATATAAGCTCTTGAAGTTTCCAAGAGTGAAAGCTCCAAATGGGTTTCACATTAGGGTTTCATCCTATTGA

Coding sequence (CDS)

ATGGCCTTAGCCATCGTAATTCTTGCTCTGCTTTTACTCCTTTGTTTTTCCGCTGCTTTGCTCCGATGGAATGAAGTTCGATACAGAAAAAAACGGTTGCCTCCTGGTACAATGGGTTGGCCTGTTTTTGGTGAAACCTCTGAGTTTCTTAAACAAGGTCCAAATTTTATGAATTCTAAAAGAGCAAGATATGGGAGCTTTTTCAAATCACATATTTTGGGATCTCCTACAGTTGTTTCTATGGATCCAGAGGTTAATAGATATATTTTAATGAATGAATCTAAAGGACTTGTCGCTGGTTATCCTCAATCTATGCTTGATATACTTGGAAAATGCAATATCGCTGCCGTACACGGTGACGCTCATAAGCTTATGAGAGGTGCTCTACTTGCTATTGTTATTGATGAGTTTATGCGATCCCATTTGCGTAATTGGGATAGTCAAATCATTGATATTCAAGAAAAAACTAAACAGATGGCTTTCTGGACATCTTTGAAGCAGACTACCAGTATCGAATCCGGTCCATTATGGGAATCTTTCACCATAGAATTCTTTAAGCTCGTCCTGGGCACTTTCTCGCTCCCTATCGATCTTCCCGGCACGAATTACCGTATAGGAGTTCAGGCAAGGAATAACATAGTAAAGCTATTGAAGCAGCTGATTAAGGAGAGAGGAAATTCAAAGATTCAAAAAGATGACATGCTTGGATATTTATTGAGTGAAGAGAACAAATATAAACTAAATGAGGAACAGATTATTGATCAAGTCATTACTGTTTTCTATTCTGGGTATGAAACTGTTTCAACAACTTCAATGATGGCTGTAAAGTTCCTTCATGATCATCCCAAAGTTCTTCAGCAACTCAGAGAAGAACATTTAGCAATAAGAGAGAAGAAAAATCCTGAGGACCCCATTGATTGGGATGATTTTAAAGCAATGGAGTTCACTCGTGCGGTGATTTTTGAAACATCAAGGCTAGCTACTGTTGTTAATGGAGTTTTGAGGAAAACTACAAAGGATTTGGAATTAAATGGGTTTCAAATTCCTAAAGGATGGAGGATTTATGTGTATACTAGAGAGATAAACTATGACCCTTTTCTCTATCCTGAACCATTTACCTTCAACCCTTGGAGATGGCTGGACAAAAACTTAGAGTCCCATAATTATTTTTTCCTGTTTGGAGGCGGAACTAGACTTTGTCCTGGCAAAGAATTGGGAATTGCAGAGGTTTCTACTTTTTTACATTACTTTGTAACTAAATACAGGTGGGAAGAAGTTGGAGGATATAAGCTCTTGAAGTTTCCAAGAGTGAAAGCTCCAAATGGGTTTCACATTAGGGTTTCATCCTATTGA

Protein sequence

MALAIVILALLLLLCFSAALLRWNEVRYRKKRLPPGTMGWPVFGETSEFLKQGPNFMNSKRARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGDAHKLMRGALLAIVIDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQTTSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLKQLIKERGNSKIQKDDMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQLREEHLAIREKKNPEDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLELNGFQIPKGWRIYVYTREINYDPFLYPEPFTFNPWRWLDKNLESHNYFFLFGGGTRLCPGKELGIAEVSTFLHYFVTKYRWEEVGGYKLLKFPRVKAPNGFHIRVSSY
Homology
BLAST of PI0001755 vs. ExPASy Swiss-Prot
Match: Q69F95 (Cytochrome P450 85A OS=Phaseolus vulgaris OX=3885 GN=BA13 PE=3 SV=2)

HSP 1 Score: 679.9 bits (1753), Expect = 2.0e-194
Identity = 332/464 (71.55%), Postives = 392/464 (84.48%), Query Frame = 0

Query: 1   MALAIVIL-ALLLLLCFSAALLRWNEVRYRKKRLPPGTMGWPVFGETSEFLKQGPNFMNS 60
           MAL + IL  L+LLLCF +ALL+WNEVR+R+K LPPG MGWPVFGET+EFLKQGPNFM +
Sbjct: 1   MALFMAILGVLVLLLCFCSALLKWNEVRFRRKGLPPGAMGWPVFGETTEFLKQGPNFMKN 60

Query: 61  KRARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHG 120
           KRARYGSFFKSHILG PT+VSMDPE+NR+ILMNE+KGLV GYPQSMLDILG  NIAAVHG
Sbjct: 61  KRARYGSFFKSHILGCPTIVSMDPELNRFILMNEAKGLVPGYPQSMLDILGTRNIAAVHG 120

Query: 121 DAHKLMRGALLAIV------------IDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQ 180
             HK MRGALL+I+            IDEFMR+HL +WD+++I+IQEKTK+MAF +SLKQ
Sbjct: 121 STHKYMRGALLSIISPTLIRDQLLPKIDEFMRTHLMDWDNKVINIQEKTKEMAFLSSLKQ 180

Query: 181 TTSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLKQLIKERGNS 240
              +ES  + + F  EFFKLVLGT SLPI+LP TNYR G+QAR +I+ +L QL++ER  S
Sbjct: 181 IAGMESSSIAQPFMTEFFKLVLGTLSLPINLPRTNYRGGLQARKSIISILSQLLEERKAS 240

Query: 241 KIQKDDMLGYLL-SEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQ 300
           +    DMLG L+  +EN+YKL++E+IID VIT+ YSGYETVSTTSMMAVK+LHDHPKVL+
Sbjct: 241 QDVHVDMLGCLMKKDENRYKLSDEEIIDLVITIMYSGYETVSTTSMMAVKYLHDHPKVLE 300

Query: 301 QLREEHLAIREKKNPEDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLELNGF 360
           ++R+EH AIRE+K PEDPID +D K+M FTRAVIFETSRLAT+VNGVLRKTT D+ELNG+
Sbjct: 301 EIRKEHFAIRERKKPEDPIDCNDLKSMRFTRAVIFETSRLATIVNGVLRKTTHDMELNGY 360

Query: 361 QIPKGWRIYVYTREINYDPFLYPEPFTFNPWRWLDKNLESHNYFFLFGGGTRLCPGKELG 420
            IPKGWRIYVYTREINYDPFLY +P TFNPWRWL  +LES ++F +FGGGTR CPGKELG
Sbjct: 361 LIPKGWRIYVYTREINYDPFLYHDPLTFNPWRWLGNSLESQSHFLIFGGGTRQCPGKELG 420

Query: 421 IAEVSTFLHYFVTKYRWEEVGGYKLLKFPRVKAPNGFHIRVSSY 451
           IAE+STFLHYFVT+YRWEEVGG KL+KFPRV APNG HIRVSS+
Sbjct: 421 IAEISTFLHYFVTRYRWEEVGGDKLMKFPRVVAPNGLHIRVSSF 464

BLAST of PI0001755 vs. ExPASy Swiss-Prot
Match: Q43147 (Cytochrome P450 85A1 OS=Solanum lycopersicum OX=4081 GN=CYP85A1 PE=2 SV=1)

HSP 1 Score: 664.8 bits (1714), Expect = 6.7e-190
Identity = 319/464 (68.75%), Postives = 388/464 (83.62%), Query Frame = 0

Query: 1   MALAIVILALLLLLC-FSAALLRWNEVRYRKKRLPPGTMGWPVFGETSEFLKQGPNFMNS 60
           MA  ++ L+    LC F  ALLRWN+V+Y +K LPPGTMGWP+FGET+EFLK GP+FM +
Sbjct: 1   MAFFLIFLSSFFGLCIFCTALLRWNQVKYNQKNLPPGTMGWPLFGETTEFLKLGPSFMKN 60

Query: 61  KRARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHG 120
           +RARYGSFFKSHILG PT+VSMD E+NRYIL+NE+KGLV GYPQSM+DILGKCNIAAV+G
Sbjct: 61  QRARYGSFFKSHILGCPTIVSMDSELNRYILVNEAKGLVPGYPQSMIDILGKCNIAAVNG 120

Query: 121 DAHKLMRGALLAIV------------IDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQ 180
            AHK MRGALL+++            IDEFMRSHL NWD+++IDIQEKT +MAF +SLKQ
Sbjct: 121 SAHKYMRGALLSLISPTMIRDQLLPKIDEFMRSHLTNWDNKVIDIQEKTNKMAFLSSLKQ 180

Query: 181 TTSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLKQLIKERGNS 240
              IES  L + F  EFF LVLGT SLPI+LP TNY  G QAR  IV LL+ LI+ER  S
Sbjct: 181 IAGIESTSLAQEFMSEFFNLVLGTLSLPINLPNTNYHRGFQARKIIVNLLRTLIEERRAS 240

Query: 241 KIQKDDMLGYLLSEE-NKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQ 300
           K  + DMLGYL++EE  ++KL ++++ID +IT+ YSGYETVSTTSMMAVK+LHDHPKVL+
Sbjct: 241 KEIQHDMLGYLMNEEATRFKLTDDEMIDLIITILYSGYETVSTTSMMAVKYLHDHPKVLE 300

Query: 301 QLREEHLAIREKKNPEDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLELNGF 360
           +LR+EH+AIREKK PEDPID++D+++M FTRAVI ETSRLAT+VNGVLRKTT+D+E+NG+
Sbjct: 301 ELRKEHMAIREKKKPEDPIDYNDYRSMRFTRAVILETSRLATIVNGVLRKTTQDMEINGY 360

Query: 361 QIPKGWRIYVYTREINYDPFLYPEPFTFNPWRWLDKNLESHNYFFLFGGGTRLCPGKELG 420
            IPKGWRIYVYTRE+NYDP LYP+P++FNPWRW+DK+LE  N F +FGGGTR CPGKELG
Sbjct: 361 IIPKGWRIYVYTRELNYDPRLYPDPYSFNPWRWMDKSLEHQNSFLVFGGGTRQCPGKELG 420

Query: 421 IAEVSTFLHYFVTKYRWEEVGGYKLLKFPRVKAPNGFHIRVSSY 451
           +AE+STFLHYFVTKYRWEE+GG KL+KFPRV+APNG  IRVS++
Sbjct: 421 VAEISTFLHYFVTKYRWEEIGGDKLMKFPRVEAPNGLRIRVSAH 464

BLAST of PI0001755 vs. ExPASy Swiss-Prot
Match: Q940V4 (Cytochrome P450 85A2 OS=Arabidopsis thaliana OX=3702 GN=CYP85A2 PE=2 SV=1)

HSP 1 Score: 639.8 bits (1649), Expect = 2.3e-182
Identity = 311/465 (66.88%), Postives = 377/465 (81.08%), Query Frame = 0

Query: 1   MALAIVILALL-LLLCFSAALLRWNEVRYRKKRLPPGTMGWPVFGETSEFLKQGPNFMNS 60
           M + ++IL LL +++C   ALLRWN++RY KK LPPGTMGWP+FGET+EFLKQGP+FM +
Sbjct: 1   MGIMMMILGLLVIIVCLCTALLRWNQMRYSKKGLPPGTMGWPIFGETTEFLKQGPDFMKN 60

Query: 61  KRARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHG 120
           +R RYGSFFKSHILG PT+VSMD E+NRYILMNESKGLVAGYPQSMLDILG CNIAAVHG
Sbjct: 61  QRLRYGSFFKSHILGCPTIVSMDAELNRYILMNESKGLVAGYPQSMLDILGTCNIAAVHG 120

Query: 121 DAHKLMRGALLAIV------------IDEFMRSHLRNWDS-QIIDIQEKTKQMAFWTSLK 180
            +H+LMRG+LL+++            ID+FMR++L  WD  + +DIQEKTK MAF +SL 
Sbjct: 121 PSHRLMRGSLLSLISPTMMKDHLLPKIDDFMRNYLCGWDDLETVDIQEKTKHMAFLSSLL 180

Query: 181 QTTSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLKQLIKERGN 240
           Q       P  E +  EFFKLV+GT S+PID+PGTNYR GVQARNNI +LL +L++ER  
Sbjct: 181 QIAETLKKPEVEEYRTEFFKLVVGTLSVPIDIPGTNYRSGVQARNNIDRLLTELMQERKE 240

Query: 241 SKIQKDDMLGYLL-SEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVL 300
           S     DMLGYL+  E+N+Y L +++I DQV+T+ YSGYETVSTTSMMA+K+LHDHPK L
Sbjct: 241 SGETFTDMLGYLMKKEDNRYLLTDKEIRDQVVTILYSGYETVSTTSMMALKYLHDHPKAL 300

Query: 301 QQLREEHLAIREKKNPEDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLELNG 360
           ++LR EHLAIRE+K P++P+  DD K+M+FTRAVIFETSRLAT+VNGVLRKTT DLELNG
Sbjct: 301 EELRREHLAIRERKRPDEPLTLDDIKSMKFTRAVIFETSRLATIVNGVLRKTTHDLELNG 360

Query: 361 FQIPKGWRIYVYTREINYDPFLYPEPFTFNPWRWLDKNLESHNYFFLFGGGTRLCPGKEL 420
           + IPKGWRIYVYTREINYD  LY +P  FNPWRW++K+LES +YF LFGGG RLCPGKEL
Sbjct: 361 YLIPKGWRIYVYTREINYDTSLYEDPMIFNPWRWMEKSLESKSYFLLFGGGVRLCPGKEL 420

Query: 421 GIAEVSTFLHYFVTKYRWEEVGGYKLLKFPRVKAPNGFHIRVSSY 451
           GI+EVS+FLHYFVTKYRWEE G  KL+ FPRV AP G+H++ S Y
Sbjct: 421 GISEVSSFLHYFVTKYRWEENGEDKLMVFPRVSAPKGYHLKCSPY 465

BLAST of PI0001755 vs. ExPASy Swiss-Prot
Match: Q9FMA5 (Cytochrome P450 85A1 OS=Arabidopsis thaliana OX=3702 GN=CYP85A1 PE=2 SV=1)

HSP 1 Score: 629.0 bits (1621), Expect = 4.1e-179
Identity = 300/463 (64.79%), Postives = 374/463 (80.78%), Query Frame = 0

Query: 2   ALAIVILALLLLLCFSAALLRWNEVRYRKKRLPPGTMGWPVFGETSEFLKQGPNFMNSKR 61
           A+ +++  LL+++   +ALLRWN++RY K  LPPGTMGWP+FGET+EFLKQGPNFM ++R
Sbjct: 3   AMMVMMGLLLIIVSLCSALLRWNQMRYTKNGLPPGTMGWPIFGETTEFLKQGPNFMRNQR 62

Query: 62  ARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGDA 121
            RYGSFFKSH+LG PT++SMD EVNRYIL NESKGLV GYPQSMLDILG CN+AAVHG +
Sbjct: 63  LRYGSFFKSHLLGCPTLISMDSEVNRYILKNESKGLVPGYPQSMLDILGTCNMAAVHGSS 122

Query: 122 HKLMRGALLAIV------------IDEFMRSHLRNWDS-QIIDIQEKTKQMAFWTSLKQT 181
           H+LMRG+LL+++            +D FMRS+L  W+  ++IDIQ+KTK MAF +SL Q 
Sbjct: 123 HRLMRGSLLSLISSTMMRDHILPKVDHFMRSYLDQWNELEVIDIQDKTKHMAFLSSLTQI 182

Query: 182 TSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLKQLIKERGNSK 241
                 P  E F   FFKLV+GT S+PIDLPGTNYR G+QARNNI +LL++L++ER +S 
Sbjct: 183 AGNLRKPFVEEFKTAFFKLVVGTLSVPIDLPGTNYRCGIQARNNIDRLLRELMQERRDSG 242

Query: 242 IQKDDMLGYLLSEE-NKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQ 301
               DMLGYL+ +E N+Y L +E+I DQV+T+ YSGYETVSTTSMMA+K+LHDHPK LQ+
Sbjct: 243 ETFTDMLGYLMKKEGNRYPLTDEEIRDQVVTILYSGYETVSTTSMMALKYLHDHPKALQE 302

Query: 302 LREEHLAIREKKNPEDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLELNGFQ 361
           LR EHLA RE+K  ++P+  +D K+M+FTRAVI+ETSRLAT+VNGVLRKTT+DLE+NG+ 
Sbjct: 303 LRAEHLAFRERKRQDEPLGLEDVKSMKFTRAVIYETSRLATIVNGVLRKTTRDLEINGYL 362

Query: 362 IPKGWRIYVYTREINYDPFLYPEPFTFNPWRWLDKNLESHNYFFLFGGGTRLCPGKELGI 421
           IPKGWRIYVYTREINYD  LY +P  FNPWRW+ K+LES N  F+FGGGTRLCPGKELGI
Sbjct: 363 IPKGWRIYVYTREINYDANLYEDPLIFNPWRWMKKSLESQNSCFVFGGGTRLCPGKELGI 422

Query: 422 AEVSTFLHYFVTKYRWEEVGGYKLLKFPRVKAPNGFHIRVSSY 451
            E+S+FLHYFVT+YRWEE+GG +L+ FPRV AP GFH+R+S Y
Sbjct: 423 VEISSFLHYFVTRYRWEEIGGDELMVFPRVFAPKGFHLRISPY 465

BLAST of PI0001755 vs. ExPASy Swiss-Prot
Match: Q50LE0 (Cytochrome P450 85A3 OS=Solanum lycopersicum OX=4081 GN=CYP85A3 PE=2 SV=1)

HSP 1 Score: 617.5 bits (1591), Expect = 1.2e-175
Identity = 297/467 (63.60%), Postives = 371/467 (79.44%), Query Frame = 0

Query: 1   MALAIVILALLLLLC-FSAALLRWNEVRYRKKRLPPGTMGWPVFGETSEFLKQGPNFMNS 60
           MA+ ++I  +    C  S  L RW ++ Y KK LPPGTMGWP+FGET EFL QGPNFM +
Sbjct: 1   MAIFLIIFVVFFGFCILSTPLFRWIDIVYNKKNLPPGTMGWPIFGETREFLNQGPNFMKN 60

Query: 61  KRARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHG 120
           +RARYG+FFKSHILG PTVVSMD  +N YIL NE+KGL+ GYPQSMLDILGKCNIAAVHG
Sbjct: 61  QRARYGNFFKSHILGCPTVVSMDAGLNVYILNNEAKGLIPGYPQSMLDILGKCNIAAVHG 120

Query: 121 DAHKLMRGALLAIV------------IDEFMRSHLRNWDS-QIIDIQEKTKQMAFWTSLK 180
             HK +RGALL+++            ID+FMRSHL  WD+  +IDIQ+ TK+MAF++SL 
Sbjct: 121 ATHKYIRGALLSLINPTMIKDHILPKIDKFMRSHLSGWDNCNVIDIQQMTKEMAFFSSLD 180

Query: 181 QTTSI-ESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLKQLIKERG 240
           Q      S  + + F   F  + LGT SLPI+ P TNY  G+Q R  IVKLL+++I++R 
Sbjct: 181 QIGGFATSSSIAQEFRAGFLNIALGTISLPINFPTTNYYRGLQGRKTIVKLLRKIIEDRR 240

Query: 241 NSKIQKDDMLGYLLSEE--NKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPK 300
            SK  + DMLG +++EE  N+Y L++E++IDQ+IT+ YSG+ETVSTTSMMAVK+LHDHPK
Sbjct: 241 GSKKIQQDMLGLMMNEEAKNRYTLSDEELIDQIITIMYSGFETVSTTSMMAVKYLHDHPK 300

Query: 301 VLQQLREEHLAIREKKNPEDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEL 360
            L+++R+EH AIREKK+ EDPID++DFKAM FTRAVI+ET RLAT+VNGVLRKTT+D+EL
Sbjct: 301 ALEEIRKEHFAIREKKSLEDPIDYNDFKAMRFTRAVIYETLRLATIVNGVLRKTTQDMEL 360

Query: 361 NGFQIPKGWRIYVYTREINYDPFLYPEPFTFNPWRWLDKNLESHNYFFLFGGGTRLCPGK 420
           NG+ IPKGWRIYVYTRE+NYDP +YP+P+TFNPWRWL+ NL+  + F +FGGGTRLCPGK
Sbjct: 361 NGYMIPKGWRIYVYTRELNYDPLIYPDPYTFNPWRWLENNLDHQSSFLMFGGGTRLCPGK 420

Query: 421 ELGIAEVSTFLHYFVTKYRWEEVGGYKLLKFPRVKAPNGFHIRVSSY 451
           ELG+AE+STFLHYFVT+YRWEEVGG KL+KFPRV+A NG  I+VS+Y
Sbjct: 421 ELGVAEISTFLHYFVTRYRWEEVGGNKLMKFPRVEALNGLWIKVSAY 467

BLAST of PI0001755 vs. ExPASy TrEMBL
Match: A0A1S3C1A8 (cytochrome P450 85A-like OS=Cucumis melo OX=3656 GN=LOC103495886 PE=3 SV=1)

HSP 1 Score: 870.5 bits (2248), Expect = 3.0e-249
Identity = 429/462 (92.86%), Postives = 438/462 (94.81%), Query Frame = 0

Query: 1   MALAIVILALLLLLCFSAALLRWNEVRYRKKRLPPGTMGWPVFGETSEFLKQGPNFMNSK 60
           MALAIVILAL LLLCF AAL +WNEVRYRK  LPPGTMGWPVFGETSEFLKQGPNFMNSK
Sbjct: 1   MALAIVILALFLLLCFCAALFQWNEVRYRKNGLPPGTMGWPVFGETSEFLKQGPNFMNSK 60

Query: 61  RARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGD 120
           RARYGSFFKSHILGSPTV+SMDPEVNRYILMNESKGLVAGYPQSMLDILG CNIAAVHGD
Sbjct: 61  RARYGSFFKSHILGSPTVISMDPEVNRYILMNESKGLVAGYPQSMLDILGTCNIAAVHGD 120

Query: 121 AHKLMRGALLAIV------------IDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT 180
           AHKLMRGALLAIV            +DEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT
Sbjct: 121 AHKLMRGALLAIVGPTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT 180

Query: 181 TSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLKQLIKERGNSK 240
           TSI SGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLK+LI ER NSK
Sbjct: 181 TSIVSGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLKKLISERVNSK 240

Query: 241 IQKDDMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL 300
           IQKDDMLGYLLSEENKYKLNEEQIIDQ+ITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL
Sbjct: 241 IQKDDMLGYLLSEENKYKLNEEQIIDQIITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHLAIREKKNPEDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLELNGFQI 360
           REEH+A+REKKNPEDPIDWDDFKAM+FTRAVIFETSRLATVVNGVLRKTTKDLE+NGF I
Sbjct: 301 REEHIALREKKNPEDPIDWDDFKAMDFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLI 360

Query: 361 PKGWRIYVYTREINYDPFLYPEPFTFNPWRWLDKNLESHNYFFLFGGGTRLCPGKELGIA 420
           PKGWRIYVYTREINYDPFLY EPFTFNPWRWLDKNLESHNYFFLFGGGTRLCPGKELGIA
Sbjct: 361 PKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFLFGGGTRLCPGKELGIA 420

Query: 421 EVSTFLHYFVTKYRWEEVGGYKLLKFPRVKAPNGFHIRVSSY 451
           EVSTFLHYFVTKYRWEEVGG KLLKFPRVKAPNGFHIRVSSY
Sbjct: 421 EVSTFLHYFVTKYRWEEVGGCKLLKFPRVKAPNGFHIRVSSY 462

BLAST of PI0001755 vs. ExPASy TrEMBL
Match: A0A0A0L0Q5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G410870 PE=3 SV=1)

HSP 1 Score: 852.4 bits (2201), Expect = 8.4e-244
Identity = 422/458 (92.14%), Postives = 431/458 (94.10%), Query Frame = 0

Query: 5   IVILALLLLLCFSAALLRWNEVRYRKKRLPPGTMGWPVFGETSEFLKQGPNFMNSKRARY 64
           +VI+ L LLLCF AALLR NEVRY K  LPPGTMGWPVFGETSEFLKQGPNFMNSKRARY
Sbjct: 1   MVIVGLFLLLCFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNSKRARY 60

Query: 65  GSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGDAHKL 124
           GSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGDAHKL
Sbjct: 61  GSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGDAHKL 120

Query: 125 MRGALLAIV------------IDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQTTSIE 184
           MRGALLAIV            +DEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQTTSIE
Sbjct: 121 MRGALLAIVSHTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQTTSIE 180

Query: 185 SGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLKQLIKERGNSKIQKD 244
           SGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLL+QLI ERGNSKIQKD
Sbjct: 181 SGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGNSKIQKD 240

Query: 245 DMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQLREEH 304
           DMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQLREEH
Sbjct: 241 DMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQLREEH 300

Query: 305 LAIREKKNPEDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLELNGFQIPKGW 364
           LA+REKKNP+DPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLE+NGF IPKGW
Sbjct: 301 LALREKKNPKDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLIPKGW 360

Query: 365 RIYVYTREINYDPFLYPEPFTFNPWRWLDKNLESHNYFFLFGGGTRLCPGKELGIAEVST 424
           RIYVY REINYDPFLY EP+TFNPWRWLDK+LESHNYFFLFGGG RLCPGKELGIAEVST
Sbjct: 361 RIYVYNREINYDPFLYSEPYTFNPWRWLDKSLESHNYFFLFGGGIRLCPGKELGIAEVST 420

Query: 425 FLHYFVTKYRWEEVGGYKLLKFPRVKAPNGFHIRVSSY 451
           FLHYFVTKYRWEEVG  KLLKFPRVKA NGFHIRVSSY
Sbjct: 421 FLHYFVTKYRWEEVGECKLLKFPRVKARNGFHIRVSSY 458

BLAST of PI0001755 vs. ExPASy TrEMBL
Match: A0A0A0KXL9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G641590 PE=3 SV=1)

HSP 1 Score: 795.4 bits (2053), Expect = 1.2e-226
Identity = 391/463 (84.45%), Postives = 420/463 (90.71%), Query Frame = 0

Query: 1   MALAIVILALLLLLCFSAALLRWNEVRYRKKRLPPGTMGWPVFGETSEFLKQGPNFMNSK 60
           MALA+VI+AL LLL F +ALLRWNEVRY KK LPPGTMGWP+FGET+EFLKQGPNFM S+
Sbjct: 1   MALAMVIIALFLLLLFCSALLRWNEVRYSKKGLPPGTMGWPIFGETTEFLKQGPNFMKSQ 60

Query: 61  RARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGD 120
           RARYGS FKSHILG PT+VSMDPEVNRY+LMNESKGLV GYPQSMLDILGKCNIAAVHG 
Sbjct: 61  RARYGSIFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGS 120

Query: 121 AHKLMRGALLAIV------------IDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT 180
           AHKLMRGALLAIV            IDEFMRSHL NWD+QIIDIQEKTK+MA  +S+KQ 
Sbjct: 121 AHKLMRGALLAIVSPTMIKDRLLPNIDEFMRSHLSNWDNQIIDIQEKTKEMALRSSMKQI 180

Query: 181 TSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLKQLIKERGNSK 240
              ESGPL ESFT EFFKLVLGT SLPIDLPGTNYR G+QAR NI+KLL+QLIKER +SK
Sbjct: 181 AGFESGPLSESFTPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLEQLIKERRDSK 240

Query: 241 IQKDDMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL 300
           IQK+DMLGY +SEENKYKLN+E+IIDQ+ITV YSGYETVSTTSMMAVKFLHDHPKVLQQL
Sbjct: 241 IQKNDMLGYFMSEENKYKLNDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHLAIRE-KKNPEDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLELNGFQ 360
           REEHLAIRE KKNPEDPIDWDD KAM+FTRAVIFETSRLAT+VNGVLRKTTKD+ELNGF 
Sbjct: 301 REEHLAIREKKKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFL 360

Query: 361 IPKGWRIYVYTREINYDPFLYPEPFTFNPWRWLDKNLESHNYFFLFGGGTRLCPGKELGI 420
           IPKGWRIYVYTREINYDPFLY EPFTFNPWRWLD NLES+NYFF+FGGGTRLCPGKELGI
Sbjct: 361 IPKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDNNLESNNYFFIFGGGTRLCPGKELGI 420

Query: 421 AEVSTFLHYFVTKYRWEEVGGYKLLKFPRVKAPNGFHIRVSSY 451
           AE+STFLHYFVTKYRWEEVGG KLLKFPRV+AP+GFHIRVSSY
Sbjct: 421 AEISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY 463

BLAST of PI0001755 vs. ExPASy TrEMBL
Match: A0A5A7TDB4 (Cytochrome P450 85A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold64G002300 PE=3 SV=1)

HSP 1 Score: 793.9 bits (2049), Expect = 3.5e-226
Identity = 390/463 (84.23%), Postives = 419/463 (90.50%), Query Frame = 0

Query: 1   MALAIVILALLLLLCFSAALLRWNEVRYRKKRLPPGTMGWPVFGETSEFLKQGPNFMNSK 60
           MALA+VILAL LLL F +ALLRWNEVRY KK LPPGTMGWP+FGET+EFLKQGPNFM S+
Sbjct: 1   MALAMVILALFLLLLFCSALLRWNEVRYSKKGLPPGTMGWPIFGETTEFLKQGPNFMKSQ 60

Query: 61  RARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGD 120
           RARYGS FKSHILG PT+VSMDPEVNRY+LMNESKGLV GYPQSMLDILGKCNIAAVHG 
Sbjct: 61  RARYGSIFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGS 120

Query: 121 AHKLMRGALLAIV------------IDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT 180
           AHKLMRGALLAIV            +DEFMRSHL NWD+QIIDIQEKTK+MA  +S+KQ 
Sbjct: 121 AHKLMRGALLAIVSPTMIKDRLLPKLDEFMRSHLSNWDNQIIDIQEKTKEMALRSSMKQI 180

Query: 181 TSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLKQLIKERGNSK 240
              ESGPL ESFT EFFKLVLGT SLPIDLPGTNYR G+QAR NI+KLL+QLIKER +SK
Sbjct: 181 AGFESGPLSESFTPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLEQLIKERRDSK 240

Query: 241 IQKDDMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL 300
           IQK+DMLGY +SEENKYKLN+E+IIDQ+ITV YSGYETVSTTSMMAVKFLHDHPKVLQQL
Sbjct: 241 IQKNDMLGYFMSEENKYKLNDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHLAIRE-KKNPEDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLELNGFQ 360
           REEHLAIRE KKNPEDPIDWDD KAM+FTRAVIFETSRLAT+VNGVLRKTTKD+ELNGF 
Sbjct: 301 REEHLAIREKKKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFL 360

Query: 361 IPKGWRIYVYTREINYDPFLYPEPFTFNPWRWLDKNLESHNYFFLFGGGTRLCPGKELGI 420
           IPKGWRIYVYTREINYDPFLY EPFTFNPWRWLD NLES+NYFF+FGGGTRLCPGKELGI
Sbjct: 361 IPKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDNNLESNNYFFIFGGGTRLCPGKELGI 420

Query: 421 AEVSTFLHYFVTKYRWEEVGGYKLLKFPRVKAPNGFHIRVSSY 451
            E+STFLHYFVTKYRWEEVGG KLLKFPRV+AP+GFHIRVSSY
Sbjct: 421 TEISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY 463

BLAST of PI0001755 vs. ExPASy TrEMBL
Match: A0A1S3CA65 (cytochrome P450 85A-like OS=Cucumis melo OX=3656 GN=LOC103498545 PE=3 SV=1)

HSP 1 Score: 793.9 bits (2049), Expect = 3.5e-226
Identity = 390/463 (84.23%), Postives = 419/463 (90.50%), Query Frame = 0

Query: 1   MALAIVILALLLLLCFSAALLRWNEVRYRKKRLPPGTMGWPVFGETSEFLKQGPNFMNSK 60
           MALA+VILAL LLL F +ALLRWNEVRY KK LPPGTMGWP+FGET+EFLKQGPNFM S+
Sbjct: 1   MALAMVILALFLLLLFCSALLRWNEVRYSKKGLPPGTMGWPIFGETTEFLKQGPNFMKSQ 60

Query: 61  RARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGD 120
           RARYGS FKSHILG PT+VSMDPEVNRY+LMNESKGLV GYPQSMLDILGKCNIAAVHG 
Sbjct: 61  RARYGSIFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGS 120

Query: 121 AHKLMRGALLAIV------------IDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT 180
           AHKLMRGALLAIV            +DEFMRSHL NWD+QIIDIQEKTK+MA  +S+KQ 
Sbjct: 121 AHKLMRGALLAIVSPTMIKDRLLPKLDEFMRSHLSNWDNQIIDIQEKTKEMALRSSMKQI 180

Query: 181 TSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLKQLIKERGNSK 240
              ESGPL ESFT EFFKLVLGT SLPIDLPGTNYR G+QAR NI+KLL+QLIKER +SK
Sbjct: 181 AGFESGPLSESFTPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLEQLIKERRDSK 240

Query: 241 IQKDDMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL 300
           IQK+DMLGY +SEENKYKLN+E+IIDQ+ITV YSGYETVSTTSMMAVKFLHDHPKVLQQL
Sbjct: 241 IQKNDMLGYFMSEENKYKLNDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHLAIRE-KKNPEDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLELNGFQ 360
           REEHLAIRE KKNPEDPIDWDD KAM+FTRAVIFETSRLAT+VNGVLRKTTKD+ELNGF 
Sbjct: 301 REEHLAIREKKKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFL 360

Query: 361 IPKGWRIYVYTREINYDPFLYPEPFTFNPWRWLDKNLESHNYFFLFGGGTRLCPGKELGI 420
           IPKGWRIYVYTREINYDPFLY EPFTFNPWRWLD NLES+NYFF+FGGGTRLCPGKELGI
Sbjct: 361 IPKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDNNLESNNYFFIFGGGTRLCPGKELGI 420

Query: 421 AEVSTFLHYFVTKYRWEEVGGYKLLKFPRVKAPNGFHIRVSSY 451
            E+STFLHYFVTKYRWEEVGG KLLKFPRV+AP+GFHIRVSSY
Sbjct: 421 TEISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY 463

BLAST of PI0001755 vs. NCBI nr
Match: XP_008455791.1 (PREDICTED: cytochrome P450 85A-like [Cucumis melo])

HSP 1 Score: 870.5 bits (2248), Expect = 6.1e-249
Identity = 429/462 (92.86%), Postives = 438/462 (94.81%), Query Frame = 0

Query: 1   MALAIVILALLLLLCFSAALLRWNEVRYRKKRLPPGTMGWPVFGETSEFLKQGPNFMNSK 60
           MALAIVILAL LLLCF AAL +WNEVRYRK  LPPGTMGWPVFGETSEFLKQGPNFMNSK
Sbjct: 1   MALAIVILALFLLLCFCAALFQWNEVRYRKNGLPPGTMGWPVFGETSEFLKQGPNFMNSK 60

Query: 61  RARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGD 120
           RARYGSFFKSHILGSPTV+SMDPEVNRYILMNESKGLVAGYPQSMLDILG CNIAAVHGD
Sbjct: 61  RARYGSFFKSHILGSPTVISMDPEVNRYILMNESKGLVAGYPQSMLDILGTCNIAAVHGD 120

Query: 121 AHKLMRGALLAIV------------IDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT 180
           AHKLMRGALLAIV            +DEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT
Sbjct: 121 AHKLMRGALLAIVGPTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT 180

Query: 181 TSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLKQLIKERGNSK 240
           TSI SGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLK+LI ER NSK
Sbjct: 181 TSIVSGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLKKLISERVNSK 240

Query: 241 IQKDDMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL 300
           IQKDDMLGYLLSEENKYKLNEEQIIDQ+ITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL
Sbjct: 241 IQKDDMLGYLLSEENKYKLNEEQIIDQIITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHLAIREKKNPEDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLELNGFQI 360
           REEH+A+REKKNPEDPIDWDDFKAM+FTRAVIFETSRLATVVNGVLRKTTKDLE+NGF I
Sbjct: 301 REEHIALREKKNPEDPIDWDDFKAMDFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLI 360

Query: 361 PKGWRIYVYTREINYDPFLYPEPFTFNPWRWLDKNLESHNYFFLFGGGTRLCPGKELGIA 420
           PKGWRIYVYTREINYDPFLY EPFTFNPWRWLDKNLESHNYFFLFGGGTRLCPGKELGIA
Sbjct: 361 PKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFLFGGGTRLCPGKELGIA 420

Query: 421 EVSTFLHYFVTKYRWEEVGGYKLLKFPRVKAPNGFHIRVSSY 451
           EVSTFLHYFVTKYRWEEVGG KLLKFPRVKAPNGFHIRVSSY
Sbjct: 421 EVSTFLHYFVTKYRWEEVGGCKLLKFPRVKAPNGFHIRVSSY 462

BLAST of PI0001755 vs. NCBI nr
Match: XP_004150514.3 (cytochrome P450 85A [Cucumis sativus])

HSP 1 Score: 855.9 bits (2210), Expect = 1.6e-244
Identity = 425/462 (91.99%), Postives = 434/462 (93.94%), Query Frame = 0

Query: 1   MALAIVILALLLLLCFSAALLRWNEVRYRKKRLPPGTMGWPVFGETSEFLKQGPNFMNSK 60
           M LA+VI+ L LLLCF AALLR NEVRY K  LPPGTMGWPVFGETSEFLKQGPNFMNSK
Sbjct: 1   MGLAMVIVGLFLLLCFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNSK 60

Query: 61  RARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGD 120
           RARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGD
Sbjct: 61  RARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGD 120

Query: 121 AHKLMRGALLAIV------------IDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT 180
           AHKLMRGALLAIV            +DEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT
Sbjct: 121 AHKLMRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT 180

Query: 181 TSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLKQLIKERGNSK 240
           TSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLL+QLI ERGNSK
Sbjct: 181 TSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGNSK 240

Query: 241 IQKDDMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL 300
           IQKDDMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL
Sbjct: 241 IQKDDMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHLAIREKKNPEDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLELNGFQI 360
           REEHLA+REKKNP+DPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLE+NGF I
Sbjct: 301 REEHLALREKKNPKDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLI 360

Query: 361 PKGWRIYVYTREINYDPFLYPEPFTFNPWRWLDKNLESHNYFFLFGGGTRLCPGKELGIA 420
           PKGWRIYVY REINYDPFLY EP+TFNPWRWLDK+LESHNYFFLFGGG RLCPGKELGIA
Sbjct: 361 PKGWRIYVYNREINYDPFLYSEPYTFNPWRWLDKSLESHNYFFLFGGGIRLCPGKELGIA 420

Query: 421 EVSTFLHYFVTKYRWEEVGGYKLLKFPRVKAPNGFHIRVSSY 451
           EVSTFLHYFVTKYRWEEVG  KLLKFPRVKA NGFHIRVSSY
Sbjct: 421 EVSTFLHYFVTKYRWEEVGECKLLKFPRVKARNGFHIRVSSY 462

BLAST of PI0001755 vs. NCBI nr
Match: XP_038890036.1 (cytochrome P450 85A-like [Benincasa hispida])

HSP 1 Score: 808.5 bits (2087), Expect = 2.9e-230
Identity = 396/462 (85.71%), Postives = 422/462 (91.34%), Query Frame = 0

Query: 1   MALAIVILALLLLLCFSAALLRWNEVRYRKKRLPPGTMGWPVFGETSEFLKQGPNFMNSK 60
           MALA+VILAL LLLCF +ALLRWNEVRY KK LPPGTMGWPVFGET+EFLKQGPNFM S+
Sbjct: 1   MALAMVILALFLLLCFCSALLRWNEVRYSKKGLPPGTMGWPVFGETTEFLKQGPNFMKSQ 60

Query: 61  RARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGD 120
           RARYGS FKSHILG PT+VSMDPEVNRY+LMNESKGLV GYPQSMLDILGKCNIAAVHG 
Sbjct: 61  RARYGSIFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG 120

Query: 121 AHKLMRGALLAIV------------IDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT 180
           AHKLMRGALLAIV            +D+FMRSHLRNWDSQIIDIQEKTK+MA  +SLKQ 
Sbjct: 121 AHKLMRGALLAIVSPTMIKDRLLPKVDDFMRSHLRNWDSQIIDIQEKTKEMALRSSLKQI 180

Query: 181 TSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLKQLIKERGNSK 240
             IESGPL ESF  EFFKLVLGT SLPIDLPGTNYR G+QAR NI+KLL+QLI ER +SK
Sbjct: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRDSK 240

Query: 241 IQKDDMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL 300
           IQKDDMLG+L+SEENKYKLN+E+IIDQ+ITV YSGYETVSTTSMMAVKFLHDHPKVLQQL
Sbjct: 241 IQKDDMLGFLMSEENKYKLNDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHLAIREKKNPEDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLELNGFQI 360
           REEHLAIREKKNPEDPIDWDD KAM+FTRAVIFETSRLAT+VNGVLRKTTKD+ELNGF I
Sbjct: 301 REEHLAIREKKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360

Query: 361 PKGWRIYVYTREINYDPFLYPEPFTFNPWRWLDKNLESHNYFFLFGGGTRLCPGKELGIA 420
           PKGWRIYVYTREINYDPFLY EPFTFNPWRWLDKNLESHNYFF+FGGGTR CPGKELGIA
Sbjct: 361 PKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGTRHCPGKELGIA 420

Query: 421 EVSTFLHYFVTKYRWEEVGGYKLLKFPRVKAPNGFHIRVSSY 451
           E+STFLHYFVTKYRWEEVGG KLLKFPRV+AP+GFHIRVSSY
Sbjct: 421 EISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY 462

BLAST of PI0001755 vs. NCBI nr
Match: XP_004141472.1 (cytochrome P450 85A [Cucumis sativus] >KGN52526.1 hypothetical protein Csa_009064 [Cucumis sativus])

HSP 1 Score: 795.4 bits (2053), Expect = 2.5e-226
Identity = 391/463 (84.45%), Postives = 420/463 (90.71%), Query Frame = 0

Query: 1   MALAIVILALLLLLCFSAALLRWNEVRYRKKRLPPGTMGWPVFGETSEFLKQGPNFMNSK 60
           MALA+VI+AL LLL F +ALLRWNEVRY KK LPPGTMGWP+FGET+EFLKQGPNFM S+
Sbjct: 1   MALAMVIIALFLLLLFCSALLRWNEVRYSKKGLPPGTMGWPIFGETTEFLKQGPNFMKSQ 60

Query: 61  RARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGD 120
           RARYGS FKSHILG PT+VSMDPEVNRY+LMNESKGLV GYPQSMLDILGKCNIAAVHG 
Sbjct: 61  RARYGSIFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGS 120

Query: 121 AHKLMRGALLAIV------------IDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT 180
           AHKLMRGALLAIV            IDEFMRSHL NWD+QIIDIQEKTK+MA  +S+KQ 
Sbjct: 121 AHKLMRGALLAIVSPTMIKDRLLPNIDEFMRSHLSNWDNQIIDIQEKTKEMALRSSMKQI 180

Query: 181 TSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLKQLIKERGNSK 240
              ESGPL ESFT EFFKLVLGT SLPIDLPGTNYR G+QAR NI+KLL+QLIKER +SK
Sbjct: 181 AGFESGPLSESFTPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLEQLIKERRDSK 240

Query: 241 IQKDDMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL 300
           IQK+DMLGY +SEENKYKLN+E+IIDQ+ITV YSGYETVSTTSMMAVKFLHDHPKVLQQL
Sbjct: 241 IQKNDMLGYFMSEENKYKLNDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHLAIRE-KKNPEDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLELNGFQ 360
           REEHLAIRE KKNPEDPIDWDD KAM+FTRAVIFETSRLAT+VNGVLRKTTKD+ELNGF 
Sbjct: 301 REEHLAIREKKKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFL 360

Query: 361 IPKGWRIYVYTREINYDPFLYPEPFTFNPWRWLDKNLESHNYFFLFGGGTRLCPGKELGI 420
           IPKGWRIYVYTREINYDPFLY EPFTFNPWRWLD NLES+NYFF+FGGGTRLCPGKELGI
Sbjct: 361 IPKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDNNLESNNYFFIFGGGTRLCPGKELGI 420

Query: 421 AEVSTFLHYFVTKYRWEEVGGYKLLKFPRVKAPNGFHIRVSSY 451
           AE+STFLHYFVTKYRWEEVGG KLLKFPRV+AP+GFHIRVSSY
Sbjct: 421 AEISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY 463

BLAST of PI0001755 vs. NCBI nr
Match: XP_008459397.1 (PREDICTED: cytochrome P450 85A-like [Cucumis melo] >KAA0039425.1 cytochrome P450 85A-like [Cucumis melo var. makuwa])

HSP 1 Score: 793.9 bits (2049), Expect = 7.3e-226
Identity = 390/463 (84.23%), Postives = 419/463 (90.50%), Query Frame = 0

Query: 1   MALAIVILALLLLLCFSAALLRWNEVRYRKKRLPPGTMGWPVFGETSEFLKQGPNFMNSK 60
           MALA+VILAL LLL F +ALLRWNEVRY KK LPPGTMGWP+FGET+EFLKQGPNFM S+
Sbjct: 1   MALAMVILALFLLLLFCSALLRWNEVRYSKKGLPPGTMGWPIFGETTEFLKQGPNFMKSQ 60

Query: 61  RARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGD 120
           RARYGS FKSHILG PT+VSMDPEVNRY+LMNESKGLV GYPQSMLDILGKCNIAAVHG 
Sbjct: 61  RARYGSIFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGS 120

Query: 121 AHKLMRGALLAIV------------IDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT 180
           AHKLMRGALLAIV            +DEFMRSHL NWD+QIIDIQEKTK+MA  +S+KQ 
Sbjct: 121 AHKLMRGALLAIVSPTMIKDRLLPKLDEFMRSHLSNWDNQIIDIQEKTKEMALRSSMKQI 180

Query: 181 TSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLKQLIKERGNSK 240
              ESGPL ESFT EFFKLVLGT SLPIDLPGTNYR G+QAR NI+KLL+QLIKER +SK
Sbjct: 181 AGFESGPLSESFTPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLEQLIKERRDSK 240

Query: 241 IQKDDMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL 300
           IQK+DMLGY +SEENKYKLN+E+IIDQ+ITV YSGYETVSTTSMMAVKFLHDHPKVLQQL
Sbjct: 241 IQKNDMLGYFMSEENKYKLNDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHLAIRE-KKNPEDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLELNGFQ 360
           REEHLAIRE KKNPEDPIDWDD KAM+FTRAVIFETSRLAT+VNGVLRKTTKD+ELNGF 
Sbjct: 301 REEHLAIREKKKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFL 360

Query: 361 IPKGWRIYVYTREINYDPFLYPEPFTFNPWRWLDKNLESHNYFFLFGGGTRLCPGKELGI 420
           IPKGWRIYVYTREINYDPFLY EPFTFNPWRWLD NLES+NYFF+FGGGTRLCPGKELGI
Sbjct: 361 IPKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDNNLESNNYFFIFGGGTRLCPGKELGI 420

Query: 421 AEVSTFLHYFVTKYRWEEVGGYKLLKFPRVKAPNGFHIRVSSY 451
            E+STFLHYFVTKYRWEEVGG KLLKFPRV+AP+GFHIRVSSY
Sbjct: 421 TEISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY 463

BLAST of PI0001755 vs. TAIR 10
Match: AT3G30180.1 (brassinosteroid-6-oxidase 2 )

HSP 1 Score: 639.8 bits (1649), Expect = 1.6e-183
Identity = 311/465 (66.88%), Postives = 377/465 (81.08%), Query Frame = 0

Query: 1   MALAIVILALL-LLLCFSAALLRWNEVRYRKKRLPPGTMGWPVFGETSEFLKQGPNFMNS 60
           M + ++IL LL +++C   ALLRWN++RY KK LPPGTMGWP+FGET+EFLKQGP+FM +
Sbjct: 1   MGIMMMILGLLVIIVCLCTALLRWNQMRYSKKGLPPGTMGWPIFGETTEFLKQGPDFMKN 60

Query: 61  KRARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHG 120
           +R RYGSFFKSHILG PT+VSMD E+NRYILMNESKGLVAGYPQSMLDILG CNIAAVHG
Sbjct: 61  QRLRYGSFFKSHILGCPTIVSMDAELNRYILMNESKGLVAGYPQSMLDILGTCNIAAVHG 120

Query: 121 DAHKLMRGALLAIV------------IDEFMRSHLRNWDS-QIIDIQEKTKQMAFWTSLK 180
            +H+LMRG+LL+++            ID+FMR++L  WD  + +DIQEKTK MAF +SL 
Sbjct: 121 PSHRLMRGSLLSLISPTMMKDHLLPKIDDFMRNYLCGWDDLETVDIQEKTKHMAFLSSLL 180

Query: 181 QTTSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLKQLIKERGN 240
           Q       P  E +  EFFKLV+GT S+PID+PGTNYR GVQARNNI +LL +L++ER  
Sbjct: 181 QIAETLKKPEVEEYRTEFFKLVVGTLSVPIDIPGTNYRSGVQARNNIDRLLTELMQERKE 240

Query: 241 SKIQKDDMLGYLL-SEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVL 300
           S     DMLGYL+  E+N+Y L +++I DQV+T+ YSGYETVSTTSMMA+K+LHDHPK L
Sbjct: 241 SGETFTDMLGYLMKKEDNRYLLTDKEIRDQVVTILYSGYETVSTTSMMALKYLHDHPKAL 300

Query: 301 QQLREEHLAIREKKNPEDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLELNG 360
           ++LR EHLAIRE+K P++P+  DD K+M+FTRAVIFETSRLAT+VNGVLRKTT DLELNG
Sbjct: 301 EELRREHLAIRERKRPDEPLTLDDIKSMKFTRAVIFETSRLATIVNGVLRKTTHDLELNG 360

Query: 361 FQIPKGWRIYVYTREINYDPFLYPEPFTFNPWRWLDKNLESHNYFFLFGGGTRLCPGKEL 420
           + IPKGWRIYVYTREINYD  LY +P  FNPWRW++K+LES +YF LFGGG RLCPGKEL
Sbjct: 361 YLIPKGWRIYVYTREINYDTSLYEDPMIFNPWRWMEKSLESKSYFLLFGGGVRLCPGKEL 420

Query: 421 GIAEVSTFLHYFVTKYRWEEVGGYKLLKFPRVKAPNGFHIRVSSY 451
           GI+EVS+FLHYFVTKYRWEE G  KL+ FPRV AP G+H++ S Y
Sbjct: 421 GISEVSSFLHYFVTKYRWEENGEDKLMVFPRVSAPKGYHLKCSPY 465

BLAST of PI0001755 vs. TAIR 10
Match: AT5G38970.1 (brassinosteroid-6-oxidase 1 )

HSP 1 Score: 629.0 bits (1621), Expect = 2.9e-180
Identity = 300/463 (64.79%), Postives = 374/463 (80.78%), Query Frame = 0

Query: 2   ALAIVILALLLLLCFSAALLRWNEVRYRKKRLPPGTMGWPVFGETSEFLKQGPNFMNSKR 61
           A+ +++  LL+++   +ALLRWN++RY K  LPPGTMGWP+FGET+EFLKQGPNFM ++R
Sbjct: 3   AMMVMMGLLLIIVSLCSALLRWNQMRYTKNGLPPGTMGWPIFGETTEFLKQGPNFMRNQR 62

Query: 62  ARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGDA 121
            RYGSFFKSH+LG PT++SMD EVNRYIL NESKGLV GYPQSMLDILG CN+AAVHG +
Sbjct: 63  LRYGSFFKSHLLGCPTLISMDSEVNRYILKNESKGLVPGYPQSMLDILGTCNMAAVHGSS 122

Query: 122 HKLMRGALLAIV------------IDEFMRSHLRNWDS-QIIDIQEKTKQMAFWTSLKQT 181
           H+LMRG+LL+++            +D FMRS+L  W+  ++IDIQ+KTK MAF +SL Q 
Sbjct: 123 HRLMRGSLLSLISSTMMRDHILPKVDHFMRSYLDQWNELEVIDIQDKTKHMAFLSSLTQI 182

Query: 182 TSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLKQLIKERGNSK 241
                 P  E F   FFKLV+GT S+PIDLPGTNYR G+QARNNI +LL++L++ER +S 
Sbjct: 183 AGNLRKPFVEEFKTAFFKLVVGTLSVPIDLPGTNYRCGIQARNNIDRLLRELMQERRDSG 242

Query: 242 IQKDDMLGYLLSEE-NKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQ 301
               DMLGYL+ +E N+Y L +E+I DQV+T+ YSGYETVSTTSMMA+K+LHDHPK LQ+
Sbjct: 243 ETFTDMLGYLMKKEGNRYPLTDEEIRDQVVTILYSGYETVSTTSMMALKYLHDHPKALQE 302

Query: 302 LREEHLAIREKKNPEDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLELNGFQ 361
           LR EHLA RE+K  ++P+  +D K+M+FTRAVI+ETSRLAT+VNGVLRKTT+DLE+NG+ 
Sbjct: 303 LRAEHLAFRERKRQDEPLGLEDVKSMKFTRAVIYETSRLATIVNGVLRKTTRDLEINGYL 362

Query: 362 IPKGWRIYVYTREINYDPFLYPEPFTFNPWRWLDKNLESHNYFFLFGGGTRLCPGKELGI 421
           IPKGWRIYVYTREINYD  LY +P  FNPWRW+ K+LES N  F+FGGGTRLCPGKELGI
Sbjct: 363 IPKGWRIYVYTREINYDANLYEDPLIFNPWRWMKKSLESQNSCFVFGGGTRLCPGKELGI 422

Query: 422 AEVSTFLHYFVTKYRWEEVGGYKLLKFPRVKAPNGFHIRVSSY 451
            E+S+FLHYFVT+YRWEE+GG +L+ FPRV AP GFH+R+S Y
Sbjct: 423 VEISSFLHYFVTRYRWEEIGGDELMVFPRVFAPKGFHLRISPY 465

BLAST of PI0001755 vs. TAIR 10
Match: AT5G38970.2 (brassinosteroid-6-oxidase 1 )

HSP 1 Score: 519.2 bits (1336), Expect = 3.2e-147
Identity = 250/384 (65.10%), Postives = 307/384 (79.95%), Query Frame = 0

Query: 81  MDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGDAHKLMRGALLAIV------- 140
           MD EVNRYIL NESKGLV GYPQSMLDILG CN+AAVHG +H+LMRG+LL+++       
Sbjct: 1   MDSEVNRYILKNESKGLVPGYPQSMLDILGTCNMAAVHGSSHRLMRGSLLSLISSTMMRD 60

Query: 141 -----IDEFMRSHLRNWDS-QIIDIQEKTKQMAFWTSLKQTTSIESGPLWESFTIEFFKL 200
                +D FMRS+L  W+  ++IDIQ+KTK MAF +SL Q       P  E F   FFKL
Sbjct: 61  HILPKVDHFMRSYLDQWNELEVIDIQDKTKHMAFLSSLTQIAGNLRKPFVEEFKTAFFKL 120

Query: 201 VLGTFSLPIDLPGTNYRIGVQARNNIVKLLKQLIKERGNSKIQKDDMLGYLLSEE-NKYK 260
           V+GT S+PIDLPGTNYR G+QARNNI +LL++L++ER +S     DMLGYL+ +E N+Y 
Sbjct: 121 VVGTLSVPIDLPGTNYRCGIQARNNIDRLLRELMQERRDSGETFTDMLGYLMKKEGNRYP 180

Query: 261 LNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQLREEHLAIREKKNPEDPID 320
           L +E+I DQV+T+ YSGYETVSTTSMMA+K+LHDHPK LQ+LR EHLA RE+K  ++P+ 
Sbjct: 181 LTDEEIRDQVVTILYSGYETVSTTSMMALKYLHDHPKALQELRAEHLAFRERKRQDEPLG 240

Query: 321 WDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLELNGFQIPKGWRIYVYTREINYDPF 380
            +D K+M+FTRAVI+ETSRLAT+VNGVLRKTT+DLE+NG+ IPKGWRIYVYTREINYD  
Sbjct: 241 LEDVKSMKFTRAVIYETSRLATIVNGVLRKTTRDLEINGYLIPKGWRIYVYTREINYDAN 300

Query: 381 LYPEPFTFNPWRWLDKNLESHNYFFLFGGGTRLCPGKELGIAEVSTFLHYFVTKYRWEEV 440
           LY +P  FNPWRW+ K+LES N  F+FGGGTRLCPGKELGI E+S+FLHYFVT+YRWEE+
Sbjct: 301 LYEDPLIFNPWRWMKKSLESQNSCFVFGGGTRLCPGKELGIVEISSFLHYFVTRYRWEEI 360

Query: 441 GGYKLLKFPRVKAPNGFHIRVSSY 451
           GG +L+ FPRV AP GFH+R+S Y
Sbjct: 361 GGDELMVFPRVFAPKGFHLRISPY 384

BLAST of PI0001755 vs. TAIR 10
Match: AT5G38970.3 (brassinosteroid-6-oxidase 1 )

HSP 1 Score: 513.8 bits (1322), Expect = 1.4e-145
Identity = 250/393 (63.61%), Postives = 314/393 (79.90%), Query Frame = 0

Query: 2   ALAIVILALLLLLCFSAALLRWNEVRYRKKRLPPGTMGWPVFGETSEFLKQGPNFMNSKR 61
           A+ +++  LL+++   +ALLRWN++RY K  LPPGTMGWP+FGET+EFLKQGPNFM ++R
Sbjct: 3   AMMVMMGLLLIIVSLCSALLRWNQMRYTKNGLPPGTMGWPIFGETTEFLKQGPNFMRNQR 62

Query: 62  ARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGDA 121
            RYGSFFKSH+LG PT++SMD EVNRYIL NESKGLV GYPQSMLDILG CN+AAVHG +
Sbjct: 63  LRYGSFFKSHLLGCPTLISMDSEVNRYILKNESKGLVPGYPQSMLDILGTCNMAAVHGSS 122

Query: 122 HKLMRGALLAIV------------IDEFMRSHLRNWDS-QIIDIQEKTKQMAFWTSLKQT 181
           H+LMRG+LL+++            +D FMRS+L  W+  ++IDIQ+KTK MAF +SL Q 
Sbjct: 123 HRLMRGSLLSLISSTMMRDHILPKVDHFMRSYLDQWNELEVIDIQDKTKHMAFLSSLTQI 182

Query: 182 TSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLKQLIKERGNSK 241
                 P  E F   FFKLV+GT S+PIDLPGTNYR G+QARNNI +LL++L++ER +S 
Sbjct: 183 AGNLRKPFVEEFKTAFFKLVVGTLSVPIDLPGTNYRCGIQARNNIDRLLRELMQERRDSG 242

Query: 242 IQKDDMLGYLLSEE-NKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQ 301
               DMLGYL+ +E N+Y L +E+I DQV+T+ YSGYETVSTTSMMA+K+LHDHPK LQ+
Sbjct: 243 ETFTDMLGYLMKKEGNRYPLTDEEIRDQVVTILYSGYETVSTTSMMALKYLHDHPKALQE 302

Query: 302 LREEHLAIREKKNPEDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLELNGFQ 361
           LR EHLA RE+K  ++P+  +D K+M+FTRAVI+ETSRLAT+VNGVLRKTT+DLE+NG+ 
Sbjct: 303 LRAEHLAFRERKRQDEPLGLEDVKSMKFTRAVIYETSRLATIVNGVLRKTTRDLEINGYL 362

Query: 362 IPKGWRIYVYTREINYDPFLYPEPFTFNPWRWL 381
           IPKGWRIYVYTREINYD  LY +P  FNPWRW+
Sbjct: 363 IPKGWRIYVYTREINYDANLYEDPLIFNPWRWM 395

BLAST of PI0001755 vs. TAIR 10
Match: AT5G05690.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 312.0 bits (798), Expect = 7.8e-85
Identity = 171/464 (36.85%), Postives = 264/464 (56.90%), Query Frame = 0

Query: 9   ALLLLLCFSAA--LLRWNEVRYRKKRLPPGTMGWPVFGETSEFL----KQGPN-FMNSKR 68
           A LLLL   AA  LL     RYR+  LPPG++G P+ GET + +     + P  F++ + 
Sbjct: 5   AFLLLLSSIAAGFLLLLRRTRYRRMGLPPGSLGLPLIGETFQLIGAYKTENPEPFIDERV 64

Query: 69  ARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGDA 128
           ARYGS F +H+ G PT+ S DPE NR++L NE K     YP S+ ++LGK ++  + G  
Sbjct: 65  ARYGSVFMTHLFGEPTIFSADPETNRFVLQNEGKLFECSYPASICNLLGKHSLLLMKGSL 124

Query: 129 HKLMRGALLAIV------------IDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQTT 188
           HK M    ++              ID  +R +L +W S+++ + E+ K++ F  ++KQ  
Sbjct: 125 HKRMHSLTMSFANSSIIKDHLMLDIDRLVRFNLDSWSSRVL-LMEEAKKITFELTVKQLM 184

Query: 189 SIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLKQLIKERGNSKI 248
           S + G   ES   E+  ++ G FSLP+ L  T YR  +QAR  + + L  ++ +R   + 
Sbjct: 185 SFDPGEWSESLRKEYLLVIEGFFSLPLPLFSTTYRKAIQARRKVAEALTVVVMKRREEEE 244

Query: 249 Q----KDDMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVL 308
           +    K DML  LL+ ++ +  ++E+I+D ++ +  +GYET ST   +AVKFL + P  L
Sbjct: 245 EGAERKKDMLAALLAADDGF--SDEEIVDFLVALLVAGYETTSTIMTLAVKFLTETPLAL 304

Query: 309 QQLREEHLAIREKKNPEDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLELNG 368
            QL+EEH  IR  K+    ++W D+K+M FT+ V+ ET R+A ++ GV R+   D+E+ G
Sbjct: 305 AQLKEEHEKIRAMKSDSYSLEWSDYKSMPFTQCVVNETLRVANIIGGVFRRAMTDVEIKG 364

Query: 369 FQIPKGWRIYVYTREINYDPFLYPEPFTFNPWRWLDKNLES--HNYFFLFGGGTRLCPGK 428
           ++IPKGW+++   R ++ DP  + +  TFNPWRW   ++ +   N F  FGGG RLCPG 
Sbjct: 365 YKIPKGWKVFSSFRAVHLDPNHFKDARTFNPWRWQSNSVTTGPSNVFTPFGGGPRLCPGY 424

Query: 429 ELGIAEVSTFLHYFVTKYRWEEVGGYKLLKFPRVKAPNGFHIRV 448
           EL    +S FLH  VT + W      KL+ FP  +    + I V
Sbjct: 425 ELARVALSVFLHRLVTGFSWVPAEQDKLVFFPTTRTQKRYPIFV 465

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q69F952.0e-19471.55Cytochrome P450 85A OS=Phaseolus vulgaris OX=3885 GN=BA13 PE=3 SV=2[more]
Q431476.7e-19068.75Cytochrome P450 85A1 OS=Solanum lycopersicum OX=4081 GN=CYP85A1 PE=2 SV=1[more]
Q940V42.3e-18266.88Cytochrome P450 85A2 OS=Arabidopsis thaliana OX=3702 GN=CYP85A2 PE=2 SV=1[more]
Q9FMA54.1e-17964.79Cytochrome P450 85A1 OS=Arabidopsis thaliana OX=3702 GN=CYP85A1 PE=2 SV=1[more]
Q50LE01.2e-17563.60Cytochrome P450 85A3 OS=Solanum lycopersicum OX=4081 GN=CYP85A3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3C1A83.0e-24992.86cytochrome P450 85A-like OS=Cucumis melo OX=3656 GN=LOC103495886 PE=3 SV=1[more]
A0A0A0L0Q58.4e-24492.14Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G410870 PE=3 SV=1[more]
A0A0A0KXL91.2e-22684.45Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G641590 PE=3 SV=1[more]
A0A5A7TDB43.5e-22684.23Cytochrome P450 85A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol... [more]
A0A1S3CA653.5e-22684.23cytochrome P450 85A-like OS=Cucumis melo OX=3656 GN=LOC103498545 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_008455791.16.1e-24992.86PREDICTED: cytochrome P450 85A-like [Cucumis melo][more]
XP_004150514.31.6e-24491.99cytochrome P450 85A [Cucumis sativus][more]
XP_038890036.12.9e-23085.71cytochrome P450 85A-like [Benincasa hispida][more]
XP_004141472.12.5e-22684.45cytochrome P450 85A [Cucumis sativus] >KGN52526.1 hypothetical protein Csa_00906... [more]
XP_008459397.17.3e-22684.23PREDICTED: cytochrome P450 85A-like [Cucumis melo] >KAA0039425.1 cytochrome P450... [more]
Match NameE-valueIdentityDescription
AT3G30180.11.6e-18366.88brassinosteroid-6-oxidase 2 [more]
AT5G38970.12.9e-18064.79brassinosteroid-6-oxidase 1 [more]
AT5G38970.23.2e-14765.10brassinosteroid-6-oxidase 1 [more]
AT5G38970.31.4e-14563.61brassinosteroid-6-oxidase 1 [more]
AT5G05690.17.8e-8536.85Cytochrome P450 superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 317..335
score: 30.77
coord: 357..381
score: 29.72
coord: 400..423
score: 24.43
coord: 390..400
score: 45.83
coord: 61..80
score: 28.18
coord: 271..297
score: 20.55
IPR001128Cytochrome P450PRINTSPR00385P450coord: 262..279
score: 29.56
coord: 318..329
score: 41.2
coord: 391..400
score: 47.04
coord: 400..411
score: 32.17
IPR001128Cytochrome P450PFAMPF00067p450coord: 189..425
e-value: 1.4E-50
score: 172.4
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 24..449
e-value: 3.3E-96
score: 324.8
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 27..446
NoneNo IPR availablePANTHERPTHR24286:SF273CYTOCHROME P450 FAMILY PROTEINcoord: 8..448
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 8..448
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 393..402

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0001755.1PI0001755.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016132 brassinosteroid biosynthetic process
biological_process GO:0010268 brassinosteroid homeostasis
biological_process GO:0016125 sterol metabolic process
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen