PI0001647 (gene) Melon (PI 482460) v1

Overview


Name	PI0001647
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	Reverse transcriptase
Location	chr01: 21062452 .. 21062937 (+)
RNA-Seq Expression	PI0001647
Synteny	PI0001647

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATAATTTTGAGGCTCAATCAATAAATACGTACGCATGGCGAGCTATGGAAGAAGAGTTGTATGACCGAGTTGCTCAACGAATGATAGATGGTTTGGCGAGAGACCTAGAGAAGAAATTTGAAATTAAGTGTCTTAAGGCATTAGGAGCTCAAGAGTTCTCAGGAACTGTAGATCTGCAGGAGGCGGAGAAGTGGATAAGAACACTTGAAAAGTGTTTCTGGGTTATGCAATGTCCAGAAGAAAGAAGGGTGGACTTGGTCGTTTTCCTACTTCTAGAAGGTGCGGAAGACTGGTGGTCTTTGGAAGAAGGAAGGAGTGGAGAAGTGAGTTGGACTGAGTTCAAGGAAATTTTCAATAAGAAGTATTTTCCCGAGGCATTTAGAGATGGGAAGCGAAATGAATTTTTGCATTTAAGGCAAGAAGATTTGTCCGTAGCTAAGTATGAGAAGAAGTTCACGAGTCCGACAAAGTATACGACATGA

mRNA sequence

ATGGATAATTTTGAGGCTCAATCAATAAATACGTACGCATGGCGAGCTATGGAAGAAGAGTTGTATGACCGAGTTGCTCAACGAATGATAGATGGTTTGGCGAGAGACCTAGAGAAGAAATTTGAAATTAAGTGTCTTAAGGCATTAGGAGCTCAAGAGTTCTCAGGAACTGTAGATCTGCAGGAGGCGGAGAAGTGGATAAGAACACTTGAAAAGTGTTTCTGGGTTATGCAATGTCCAGAAGAAAGAAGGGTGGACTTGGTCGTTTTCCTACTTCTAGAAGGTGCGGAAGACTGGTGGTCTTTGGAAGAAGGAAGGAGTGGAGAAGTGAGTTGGACTGAGTTCAAGGAAATTTTCAATAAGAAGTATTTTCCCGAGGCATTTAGAGATGGGAAGCGAAATGAATTTTTGCATTTAAGGCAAGAAGATTTGTCCGTAGCTAAGTATGAGAAGAAGTTCACGAGTCCGACAAAGTATACGACATGA

Coding sequence (CDS)

ATGGATAATTTTGAGGCTCAATCAATAAATACGTACGCATGGCGAGCTATGGAAGAAGAGTTGTATGACCGAGTTGCTCAACGAATGATAGATGGTTTGGCGAGAGACCTAGAGAAGAAATTTGAAATTAAGTGTCTTAAGGCATTAGGAGCTCAAGAGTTCTCAGGAACTGTAGATCTGCAGGAGGCGGAGAAGTGGATAAGAACACTTGAAAAGTGTTTCTGGGTTATGCAATGTCCAGAAGAAAGAAGGGTGGACTTGGTCGTTTTCCTACTTCTAGAAGGTGCGGAAGACTGGTGGTCTTTGGAAGAAGGAAGGAGTGGAGAAGTGAGTTGGACTGAGTTCAAGGAAATTTTCAATAAGAAGTATTTTCCCGAGGCATTTAGAGATGGGAAGCGAAATGAATTTTTGCATTTAAGGCAAGAAGATTTGTCCGTAGCTAAGTATGAGAAGAAGTTCACGAGTCCGACAAAGTATACGACATGA

Protein sequence

MDNFEAQSINTYAWRAMEEELYDRVAQRMIDGLARDLEKKFEIKCLKALGAQEFSGTVDLQEAEKWIRTLEKCFWVMQCPEERRVDLVVFLLLEGAEDWWSLEEGRSGEVSWTEFKEIFNKKYFPEAFRDGKRNEFLHLRQEDLSVAKYEKKFTSPTKYTT

Homology

[Close]

BLAST of PI0001647 vs. ExPASy TrEMBL
Match: A0A5A7U067 (Retrotrans_gag domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold648G00470 PE=4 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 1.9e-33
Identity = 77/163 (47.24%), Postives = 110/163 (67.48%), Query Frame = 0


Query: 5   EAQSINTYAWRAMEEELYDRVAQRMIDGL---ARDLEKKFEIKCLKALGAQEFSGTVDLQ 64
           +A+S   +    +EE+L DR+AQ ++ G+     +LEK F I+ LKALGA  F+GT +L 
Sbjct: 29  DAESSRLHVEVNVEEQLLDRLAQGLVSGIRSAQSNLEKNFWIERLKALGATTFAGTTNLA 88

Query: 65  EAEKWIRTLEKCFWVMQCPEERRVDLVVFLLLEGAEDWWSLEEGR---SGEVSWTEFKEI 124
           +AE W+  +EKCF VM+C E+R+V+LVVFLL  G EDWW L E R   + ++SW EFK+ 
Sbjct: 89  DAEAWLTLIEKCFKVMRCLEDRKVELVVFLLKNGTEDWWRLTESRRRATVDMSWDEFKKT 148

Query: 125 FNKKYFPEAFRDGKRNEFLHLRQEDLSVAKYEKKFTSPTKYTT 162
           F +K++  +FRD KR++FL   Q  + V KYEKK+T  +KY T
Sbjct: 149 FFEKFYSRSFRDAKRSKFLRFTQGSMIVVKYEKKYTKLSKYAT 191

[Close]

BLAST of PI0001647 vs. ExPASy TrEMBL
Match: A0A5A7UZM6 (Gag protease polyprotein-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold119G00750 PE=4 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 2.1e-32
Identity = 68/129 (52.71%), Postives = 93/129 (72.09%), Query Frame = 0


Query: 36  DLEKKFEIKCLKALGAQEFSGTVDLQEAEKWIRTLEKCFWVMQCPEERRVDLVVFLLLEG 95
           D EKK+ I+ LKALGA  F+GT +  +AE W+  +EKCF V +CPE+R+V+L  FLL  G
Sbjct: 51  DPEKKYGIERLKALGATTFAGTTNPADAEAWLTLIEKCFRVTRCPEDRKVELAAFLLQNG 110

Query: 96  AEDWWSLEEGR---SGEVSWTEFKEIFNKKYFPEAFRDGKRNEFLHLRQEDLSVAKYEKK 155
           AEDWW +EE R   +G++SW EFK+ F  K++P +FRD KRNEFL L Q  +++A+YEKK
Sbjct: 111 AEDWWRMEESRRRTTGDISWNEFKKAFFDKFYPRSFRDAKRNEFLRLTQGSMTIAEYEKK 170

Query: 156 FTSPTKYTT 162
           +T  + Y T
Sbjct: 171 YTELSMYAT 179

[Close]

BLAST of PI0001647 vs. ExPASy TrEMBL
Match: A0A5D3BB91 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold110G001760 PE=4 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 2.8e-32
Identity = 74/163 (45.40%), Postives = 107/163 (65.64%), Query Frame = 0


Query: 5   EAQSINTYAWRAMEEELYDRVAQRMIDGL---ARDLEKKFEIKCLKALGAQEFSGTVDLQ 64
           +A+S        +EE+L DR+AQR++ G+     D EKK+  + LKALGA  F+GT +  
Sbjct: 29  DAESSRPRVEENVEEQLLDRLAQRLVSGIRSAQSDPEKKYGFERLKALGATTFAGTTNPT 88

Query: 65  EAEKWIRTLEKCFWVMQCPEERRVDLVVFLLLEGAEDWWSLEEGR---SGEVSWTEFKEI 124
           + E W+  +EKCF V +  E+R+V+L  FLL   AEDWW +EE R   +G++SW EFK+ 
Sbjct: 89  DVEAWLTLIEKCFRVTRYLEDRKVELAAFLLQNDAEDWWRMEESRRRTTGDMSWDEFKKA 148

Query: 125 FNKKYFPEAFRDGKRNEFLHLRQEDLSVAKYEKKFTSPTKYTT 162
           F  K++P +FRD K NEF+ L Q  ++VA+YEKK+T  +KY T
Sbjct: 149 FFDKFYPRSFRDAKHNEFVRLTQGTMTVAEYEKKYTELSKYAT 191

[Close]

BLAST of PI0001647 vs. ExPASy TrEMBL
Match: A0A5A7TBS0 (CCHC-type domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold64G002900 PE=4 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 3.6e-32
Identity = 68/129 (52.71%), Postives = 93/129 (72.09%), Query Frame = 0


Query: 36  DLEKKFEIKCLKALGAQEFSGTVDLQEAEKWIRTLEKCFWVMQCPEERRVDLVVFLLLEG 95
           D +KK+ I+ LKALGA  F+GT +  + E W+  +EKCF V +CPE+R+V+L  FLL  G
Sbjct: 145 DPKKKYGIERLKALGATTFAGTTNPTDVEAWLTLIEKCFRVTRCPEDRKVELAAFLLQNG 204

Query: 96  AEDWWSLEEGR---SGEVSWTEFKEIFNKKYFPEAFRDGKRNEFLHLRQEDLSVAKYEKK 155
           AEDWW +EE R   +G++SW EFK+ F  K++P +FRD KRNEFL L Q  ++VA+YEKK
Sbjct: 205 AEDWWRMEESRRRTTGDISWDEFKKAFFDKFYPRSFRDAKRNEFLRLTQGSMTVAEYEKK 264

Query: 156 FTSPTKYTT 162
           +T  +KY T
Sbjct: 265 YTELSKYAT 273

[Close]

BLAST of PI0001647 vs. ExPASy TrEMBL
Match: A0A5D3CTK6 (CCHC-type domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold892G00030 PE=4 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 3.6e-32
Identity = 68/129 (52.71%), Postives = 93/129 (72.09%), Query Frame = 0


Query: 36  DLEKKFEIKCLKALGAQEFSGTVDLQEAEKWIRTLEKCFWVMQCPEERRVDLVVFLLLEG 95
           D +KK+ I+ LKALGA  F+GT +  + E W+  +EKCF V +CPE+R+V+L  FLL  G
Sbjct: 181 DPKKKYGIERLKALGATTFAGTTNPTDVEAWLTLIEKCFRVTRCPEDRKVELAAFLLQNG 240

Query: 96  AEDWWSLEEGR---SGEVSWTEFKEIFNKKYFPEAFRDGKRNEFLHLRQEDLSVAKYEKK 155
           AEDWW +EE R   +G++SW EFK+ F  K++P +FRD KRNEFL L Q  ++VA+YEKK
Sbjct: 241 AEDWWRMEESRRRTTGDISWDEFKKAFFDKFYPRSFRDAKRNEFLRLTQGSMTVAEYEKK 300

Query: 156 FTSPTKYTT 162
           +T  +KY T
Sbjct: 301 YTELSKYAT 309

[Close]

BLAST of PI0001647 vs. NCBI nr
Match: KAA0047616.1 (uncharacterized protein E6C27_scaffold115G00890 [Cucumis melo var. makuwa] >TYK08273.1 uncharacterized protein E5676_scaffold648G00470 [Cucumis melo var. makuwa])

HSP 1 Score: 152.1 bits (383), Expect = 4.0e-33
Identity = 77/163 (47.24%), Postives = 110/163 (67.48%), Query Frame = 0


Query: 5   EAQSINTYAWRAMEEELYDRVAQRMIDGL---ARDLEKKFEIKCLKALGAQEFSGTVDLQ 64
           +A+S   +    +EE+L DR+AQ ++ G+     +LEK F I+ LKALGA  F+GT +L 
Sbjct: 29  DAESSRLHVEVNVEEQLLDRLAQGLVSGIRSAQSNLEKNFWIERLKALGATTFAGTTNLA 88

Query: 65  EAEKWIRTLEKCFWVMQCPEERRVDLVVFLLLEGAEDWWSLEEGR---SGEVSWTEFKEI 124
           +AE W+  +EKCF VM+C E+R+V+LVVFLL  G EDWW L E R   + ++SW EFK+ 
Sbjct: 89  DAEAWLTLIEKCFKVMRCLEDRKVELVVFLLKNGTEDWWRLTESRRRATVDMSWDEFKKT 148

Query: 125 FNKKYFPEAFRDGKRNEFLHLRQEDLSVAKYEKKFTSPTKYTT 162
           F +K++  +FRD KR++FL   Q  + V KYEKK+T  +KY T
Sbjct: 149 FFEKFYSRSFRDAKRSKFLRFTQGSMIVVKYEKKYTKLSKYAT 191

[Close]

BLAST of PI0001647 vs. NCBI nr
Match: KAA0060484.1 (Gag protease polyprotein-like protein [Cucumis melo var. makuwa] >TYK18569.1 Gag protease polyprotein-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 148.7 bits (374), Expect = 4.4e-32
Identity = 68/129 (52.71%), Postives = 93/129 (72.09%), Query Frame = 0


Query: 36  DLEKKFEIKCLKALGAQEFSGTVDLQEAEKWIRTLEKCFWVMQCPEERRVDLVVFLLLEG 95
           D EKK+ I+ LKALGA  F+GT +  +AE W+  +EKCF V +CPE+R+V+L  FLL  G
Sbjct: 51  DPEKKYGIERLKALGATTFAGTTNPADAEAWLTLIEKCFRVTRCPEDRKVELAAFLLQNG 110

Query: 96  AEDWWSLEEGR---SGEVSWTEFKEIFNKKYFPEAFRDGKRNEFLHLRQEDLSVAKYEKK 155
           AEDWW +EE R   +G++SW EFK+ F  K++P +FRD KRNEFL L Q  +++A+YEKK
Sbjct: 111 AEDWWRMEESRRRTTGDISWNEFKKAFFDKFYPRSFRDAKRNEFLRLTQGSMTIAEYEKK 170

Query: 156 FTSPTKYTT 162
           +T  + Y T
Sbjct: 171 YTELSMYAT 179

[Close]

BLAST of PI0001647 vs. NCBI nr
Match: TYJ95881.1 (retrotransposon protein, putative, Ty3-gypsy subclass [Cucumis melo var. makuwa])

HSP 1 Score: 148.3 bits (373), Expect = 5.7e-32
Identity = 74/163 (45.40%), Postives = 107/163 (65.64%), Query Frame = 0


Query: 5   EAQSINTYAWRAMEEELYDRVAQRMIDGL---ARDLEKKFEIKCLKALGAQEFSGTVDLQ 64
           +A+S        +EE+L DR+AQR++ G+     D EKK+  + LKALGA  F+GT +  
Sbjct: 29  DAESSRPRVEENVEEQLLDRLAQRLVSGIRSAQSDPEKKYGFERLKALGATTFAGTTNPT 88

Query: 65  EAEKWIRTLEKCFWVMQCPEERRVDLVVFLLLEGAEDWWSLEEGR---SGEVSWTEFKEI 124
           + E W+  +EKCF V +  E+R+V+L  FLL   AEDWW +EE R   +G++SW EFK+ 
Sbjct: 89  DVEAWLTLIEKCFRVTRYLEDRKVELAAFLLQNDAEDWWRMEESRRRTTGDMSWDEFKKA 148

Query: 125 FNKKYFPEAFRDGKRNEFLHLRQEDLSVAKYEKKFTSPTKYTT 162
           F  K++P +FRD K NEF+ L Q  ++VA+YEKK+T  +KY T
Sbjct: 149 FFDKFYPRSFRDAKHNEFVRLTQGTMTVAEYEKKYTELSKYAT 191

[Close]

BLAST of PI0001647 vs. NCBI nr
Match: TYK15233.1 (uncharacterized protein E5676_scaffold892G00030 [Cucumis melo var. makuwa])

HSP 1 Score: 147.9 bits (372), Expect = 7.5e-32
Identity = 68/129 (52.71%), Postives = 93/129 (72.09%), Query Frame = 0


Query: 36  DLEKKFEIKCLKALGAQEFSGTVDLQEAEKWIRTLEKCFWVMQCPEERRVDLVVFLLLEG 95
           D +KK+ I+ LKALGA  F+GT +  + E W+  +EKCF V +CPE+R+V+L  FLL  G
Sbjct: 181 DPKKKYGIERLKALGATTFAGTTNPTDVEAWLTLIEKCFRVTRCPEDRKVELAAFLLQNG 240

Query: 96  AEDWWSLEEGR---SGEVSWTEFKEIFNKKYFPEAFRDGKRNEFLHLRQEDLSVAKYEKK 155
           AEDWW +EE R   +G++SW EFK+ F  K++P +FRD KRNEFL L Q  ++VA+YEKK
Sbjct: 241 AEDWWRMEESRRRTTGDISWDEFKKAFFDKFYPRSFRDAKRNEFLRLTQGSMTVAEYEKK 300

Query: 156 FTSPTKYTT 162
           +T  +KY T
Sbjct: 301 YTELSKYAT 309

[Close]

BLAST of PI0001647 vs. NCBI nr
Match: KAA0039476.1 (uncharacterized protein E6C27_scaffold64G002900 [Cucumis melo var. makuwa])

HSP 1 Score: 147.9 bits (372), Expect = 7.5e-32
Identity = 68/129 (52.71%), Postives = 93/129 (72.09%), Query Frame = 0


Query: 36  DLEKKFEIKCLKALGAQEFSGTVDLQEAEKWIRTLEKCFWVMQCPEERRVDLVVFLLLEG 95
           D +KK+ I+ LKALGA  F+GT +  + E W+  +EKCF V +CPE+R+V+L  FLL  G
Sbjct: 145 DPKKKYGIERLKALGATTFAGTTNPTDVEAWLTLIEKCFRVTRCPEDRKVELAAFLLQNG 204

Query: 96  AEDWWSLEEGR---SGEVSWTEFKEIFNKKYFPEAFRDGKRNEFLHLRQEDLSVAKYEKK 155
           AEDWW +EE R   +G++SW EFK+ F  K++P +FRD KRNEFL L Q  ++VA+YEKK
Sbjct: 205 AEDWWRMEESRRRTTGDISWDEFKKAFFDKFYPRSFRDAKRNEFLRLTQGSMTVAEYEKK 264

Query: 156 FTSPTKYTT 162
           +T  +KY T
Sbjct: 265 YTELSKYAT 273

The following BLAST results are available for this feature:

BLAST of PI0001647 vs.


Match Name	E-value	Identity	Description

BLAST of PI0001647 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7U067	1.9e-33	47.24	Retrotrans_gag domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 ...	[more]
A0A5A7UZM6	2.1e-32	52.71	Gag protease polyprotein-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]
A0A5D3BB91	2.8e-32	45.40	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold11...	[more]
A0A5A7TBS0	3.6e-32	52.71	CCHC-type domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6...	[more]
A0A5D3CTK6	3.6e-32	52.71	CCHC-type domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5...	[more]

BLAST of PI0001647 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0047616.1	4.0e-33	47.24	uncharacterized protein E6C27_scaffold115G00890 [Cucumis melo var. makuwa] >TYK0...	[more]
KAA0060484.1	4.4e-32	52.71	Gag protease polyprotein-like protein [Cucumis melo var. makuwa] >TYK18569.1 Gag...	[more]
TYJ95881.1	5.7e-32	45.40	retrotransposon protein, putative, Ty3-gypsy subclass [Cucumis melo var. makuwa]	[more]
TYK15233.1	7.5e-32	52.71	uncharacterized protein E5676_scaffold892G00030 [Cucumis melo var. makuwa]	[more]
KAA0039476.1	7.5e-32	52.71	uncharacterized protein E6C27_scaffold64G002900 [Cucumis melo var. makuwa]	[more]

BLAST of PI0001647 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005162	Retrotransposon gag domain	PFAM	PF03732	Retrotrans_gag	coord: 87..155 e-value: 3.9E-11 score: 43.1
None	No IPR available	PANTHER	PTHR34482:SF4	POLYMERASES SUPERFAMILY PROTEIN, PUTATIVE ISOFORM 1-RELATED	coord: 61..151
None	No IPR available	PANTHER	PTHR34482	DNA DAMAGE-INDUCIBLE PROTEIN 1-LIKE	coord: 61..151

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0001647.1	PI0001647.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090304	nucleic acid metabolic process
molecular_function	GO:0005488	binding
molecular_function	GO:0008233	peptidase activity
molecular_function	GO:0016740	transferase activity