PI0001531 (gene) Melon (PI 482460) v1

Overview


Name	PI0001531
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	4-coumarate-CoA ligase
Location	chr04: 16558358 .. 16562595 (-)
RNA-Seq Expression	PI0001531
Synteny	PI0001531

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGATTATGAGTTTTATTCTTTTGATCACTGAATATTTAGTTTACAATGTAATGTTTTGATATTTGTTTGCTTGTTCTTTTCTTTCAGTTTATGAAAAATGGCACCGCTCTTAAACGATCATTTGATCAGTTTTGAAACGCCAGAAAATGAGGCTGTTATCACTAAAAAAATTCCTCGATCTGAACGCACCAACAAATATCCTAATTGGTATTCACCAGATACAGGAATTTGTCATAGTGTCCATGGCTACAGAAAACTTCCCTCTGATCCATTTCTTGATGTTGTCTCCTTCATCTTCTCCTTCCAGTTCCAACACAATGGACATTCAGCTCTCATTGATTCCTCAACTGGAAATTCAATATCTTACAAAGAATTGTTTCCTATGGTAAAATCCATGGCTTCTGGTCTCCATAACTTAGGTATATCACAAGGGGATGTAGTTTTGCTTATGTTACCAAATTCAATTTTCTATCCCATTATTATTTTGGGTGCCCTGTATTTGGGAGCTGTTATTACAACCATGTTTCCTCAAAGCAGTTCCTCTGAAATCAAGAAACGAATCACTGATTGCAATGTGCGTTTGGCTTTTACAATAACTCAGAAAATCAAGAATTTTGAAGCATTGGGAATTAAGACAATTGGTGTACCAGAAAATACGGACTTCGACTTGATGAAGCCTATGGGATTTTCTTCTTTTTATGAACTTATTTCAGGTGGTTCTGATTTGATTAAAAAGCCAGTGATCAGACAAGAAGATACAGCTGCTATATTGTTTTCTTCAGGTACTACTGGAGTGAGCAAAGGGGTTATGTTATCACATAGAAATTTTATATCTACGATCGAGCTTTTCGTTCGGTTTGAAGCTTCACAGTATGAATATTTGCCTACAAAGAATGTGTATTTGGCTGCTGTACCAATGTTTCATATCTATGGTCTCTCCATTTTTGTGATGGGATTAATGTCATTGGGGTCTAGTGTTGTTGTAATGAGCAAATTTGATGTCAAAGATGTGGTCAAGGCCATTGATAGATTTAAGGTCACACATTTTCCAGTTGTTCCACCTATATTGACAGCTTTGACTAGGACTGCAGAGAAAATTGGCGTGCAAAGATTTCGAAGTTTGAAACAAGTTTCTTGTGGGGCTGCTTCTTCAAGCAAGAAGACCATAGATGATTTTGTGCACGCTCTCCCTCATGTTGACTTCATTCAGGTATTGATTAAACCAATTCTGTTTATGATGGATTGAGAATAACTTAAAACAGGCTTAATTTAAAAAACAAAAGGGTGAAAATTGGCTTAATTTTCAAAAATAAAAAACAAAAAACATAATGGGTCTTACTTTTGTTTGTGAAGCATCCAAAGCAATGTCTGAGTCCTGTTCTGTATCTTGTTTGGAAATAGAGGAACTTTGACTCATAATGTTGATTTGCTATGTTTGTTAGTTTGAACAATGCTTGGTTTGGTGTATTTGTGGTTAGGGATATGGCATGACAGAGTCCACAGCTATAGGAACCCGTGGTTTCAATACCAAAAATAATGCTAGAAATTATTTGTCAGTGGGACTCTTAGCTCCAAACACAGAAGCTAGAGTCGTCGATTTGGTTGCTGGATCTTGCATGCCTCCTGGTGAAACCGGCGAACTTTTGTTACGTGGACCTGGTTCAATGAAAGGTTTAATTGATTCTTTCCAAAACAATCTGTGCATTGCCCAAAGATCTTGTTAACTACCATTTACTTTAAAGTTTAGACTAATAGGTTTTGATATATCTTTTGTTTATATTACTACTAAATCTAAAGGTTGAAGTTGATGAGTTATAACTCACAACATATTTAATACTTTGTTTCTATTCTTAACCCATCTATTTGTTGGCTGTAGTGAATTTCACATTTCTCTTCCGTATTTTTCTGTCACTTTCATCAGTCTAGAGTTCATAGCCGTCTCCATTACTGTGCATCTACCCTCACCATTAAAGAAGCATGAATATGGATATGGCATAACATAAACATAGTGACATGCCATATTTTAGACATCTAGAGCACTACTCGGAGATGCCACGTTTATTAGAATACACATTTTTCAAAATATATATCATTTCTATACAAAAAGAAAATTTAAAGCAAATGGATTGAATGTTTATGAATGTATATACTCAGAAAACTTAGCTTTGATGTATGTCTCATTCAAAAGTTATTATTAATTATTGTCATGTATGTGTCTTTTCAGTATACTCAACAAGTATTCTATGCATGTCTAACACATTTGTTGTATTAACAAGTGTTTGATACGTGTCCAACAAGTATATATAGGAGTGTGTTGAACACAGACACGCAATGTCAAACTGAAGTGTTTGTGCTTCTTTGCAGTCACCACGATCACAAAGATATGTCACTAACTACAGTTGTTTTGTTTTGTATTTATAGGGTACTTGAATAATCCAGAGGCAACAATGTCCACAATAGACCAAGAAAATTGGCTACATACTGGAGACATTGTCTACTTTGACCGAGATGGATACTTGTATGTTGTTGATCGTTTGAAGGAGGTTATAAAGTATAAAGGCTTTCAGGTACAATATAATAATCCAAATTTTTTTTTTTTTACGTTGTCAAAGATACTGCTACAGCTTATGAGTTATCACGCTCATGAACGTTTAGAGTGATTTACATTTGTTTTTAGCTAAGGACAATTATCAGAGATGACAAAATTTAGACCTATCACTAGTAAGAGTCTATCACTGATAGATTATATCATTAGTAGAAGCCTATCAACGATAAAATCTATCATCAATATATTTTGCTATATTTGTAATTCTATAAAAATGTTGCTATACACTTAATTATTATTTTGAAAATTGCTACCTAATGTTCAATTATCCTAGCTTTTTATATAATTGACGTTTAACTCCTCAGCCAACGAAAATAAAACATGGTTACCAAACCAAACAGAGCTTTAAATACATAAAAGTTTAACCTTCGAGGAGTTAAAGAATTGATTTTTCGCTCTGATCTTGCTTGTGGTGTGTCAATGCTTTGAATGAAGTTCATGATGGATAAAGTTAGTAATGTAATTTGCTTAATCTGTTATTTTGTAGGTTGCACCTACTGATTTAGAAGCTGTAGTGATCACACATCCCGAAGTTCTTGATACTGCAGTAGCAGCGTGAGTATTATTCTAATCACCACACACAATTTTAAGATAGACAGTAAATATCTTTTTAGTGAGTTACTTTGTCATTTCAGTCAAAACTAATACACTTTAAAATCTAGCTAGGCGACATTAAATTATTCTAAAAGTGTCTCCATATATAACTTTTAAGTGAATATAAAAACATCCCTAGATCTATGAAGAAGATTGTAAATAAATATATCAAAGAAAAAATTTAGAAATATCTCAAGCTCTCTTTAATCTCGAAGGAATTCTAAAACTCTTTTAGACTAGGATAATGTCATAAAAATATCTTGAATAGTTGAGATAAAAAAAGTATTGAAAAATCCAATGGCTAAGCTCAAAGATCTCACACCTATAAATAGGATGATTTATTCTCATTTGTAAAGCAAATAAAGAAAAGAAAAGAAGAAAGATAGCGAAGCTACTAAGTTAGAAAGTTAGACAAACATAGATGAAGAAAATTGAGTGGCCATCTTTGTAGATTCTTAATAAAAGTTCCTTTGTTCCACGTGATTTCTCTCTTTTTTTTCTTTTTTGCCAATTTCTTCAACACAAGTGTCAAACAAGAAAACACAAATAAATTGGGGGATAAAGCGACGTCAATCCTTGTAAATTTTAATCATCATCAGACAAGTTAATTTTGATGCTGTTTTAATTTTGAGATAATTTCAGATCATATCTTAGAGGCATTTGCCATGTTTTTTTCAGGGCCAAAGATGAAGAATGTGGTGAAATTCCAGTGGCATTTGTGGTAAAGAAGCCAGGAAGTGCACTCTCACAAAAAGATGTTGTTGATTATGTTGCTCAACAGGTTTCCCATATTTTTCGAATCTGTCCATGCATTTTTAAACATTAAAAATGTTCGTATTATATATATATTCGTCTTTTGTCTCTGCAGGTTGCACCATATAAAAGAGTTAGAAAAGTGATTTTTACGGAATCAATACCAAAGTCTGCTGCTGGGAAAGTACTTCGAAGGGAACTACAGAAACACTTGCCAGCTTCTAAATTATGATGAGCTCTCCAGCCAATGTTTTTGTTCCTTTCTTTTCCTTATTACTAATTGGC

mRNA sequence

AAGATTATGAGTTTTATTCTTTTGATCACTGAATATTTAGTTTACAATGTAATGTTTTGATATTTGTTTGCTTGTTCTTTTCTTTCAGTTTATGAAAAATGGCACCGCTCTTAAACGATCATTTGATCAGTTTTGAAACGCCAGAAAATGAGGCTGTTATCACTAAAAAAATTCCTCGATCTGAACGCACCAACAAATATCCTAATTGGTATTCACCAGATACAGGAATTTGTCATAGTGTCCATGGCTACAGAAAACTTCCCTCTGATCCATTTCTTGATGTTGTCTCCTTCATCTTCTCCTTCCAGTTCCAACACAATGGACATTCAGCTCTCATTGATTCCTCAACTGGAAATTCAATATCTTACAAAGAATTGTTTCCTATGGTAAAATCCATGGCTTCTGGTCTCCATAACTTAGGTATATCACAAGGGGATGTAGTTTTGCTTATGTTACCAAATTCAATTTTCTATCCCATTATTATTTTGGGTGCCCTGTATTTGGGAGCTGTTATTACAACCATGTTTCCTCAAAGCAGTTCCTCTGAAATCAAGAAACGAATCACTGATTGCAATGTGCGTTTGGCTTTTACAATAACTCAGAAAATCAAGAATTTTGAAGCATTGGGAATTAAGACAATTGGTGTACCAGAAAATACGGACTTCGACTTGATGAAGCCTATGGGATTTTCTTCTTTTTATGAACTTATTTCAGGTGGTTCTGATTTGATTAAAAAGCCAGTGATCAGACAAGAAGATACAGCTGCTATATTGTTTTCTTCAGGTACTACTGGAGTGAGCAAAGGGGTTATGTTATCACATAGAAATTTTATATCTACGATCGAGCTTTTCGTTCGGTTTGAAGCTTCACAGTATGAATATTTGCCTACAAAGAATGTGTATTTGGCTGCTGTACCAATGTTTCATATCTATGGTCTCTCCATTTTTGTGATGGGATTAATGTCATTGGGGTCTAGTGTTGTTGTAATGAGCAAATTTGATGTCAAAGATGTGGTCAAGGCCATTGATAGATTTAAGGTCACACATTTTCCAGTTGTTCCACCTATATTGACAGCTTTGACTAGGACTGCAGAGAAAATTGGCGTGCAAAGATTTCGAAGTTTGAAACAAGTTTCTTGTGGGGCTGCTTCTTCAAGCAAGAAGACCATAGATGATTTTGTGCACGCTCTCCCTCATGTTGACTTCATTCAGGGATATGGCATGACAGAGTCCACAGCTATAGGAACCCGTGGTTTCAATACCAAAAATAATGCTAGAAATTATTTGTCAGTGGGACTCTTAGCTCCAAACACAGAAGCTAGAGTCGTCGATTTGGTTGCTGGATCTTGCATGCCTCCTGGTGAAACCGGCGAACTTTTGTTACGTGGACCTGGTTCAATGAAAGGGTACTTGAATAATCCAGAGGCAACAATGTCCACAATAGACCAAGAAAATTGGCTACATACTGGAGACATTGTCTACTTTGACCGAGATGGATACTTGTATGTTGTTGATCGTTTGAAGGAGGTTATAAAGTATAAAGGCTTTCAGGTTGCACCTACTGATTTAGAAGCTGTAGTGATCACACATCCCGAAGTTCTTGATACTGCAGTAGCAGCGGCCAAAGATGAAGAATGTGGTGAAATTCCAGTGGCATTTGTGGTAAAGAAGCCAGGAAGTGCACTCTCACAAAAAGATGTTGTTGATTATGTTGCTCAACAGGTTGCACCATATAAAAGAGTTAGAAAAGTGATTTTTACGGAATCAATACCAAAGTCTGCTGCTGGGAAAGTACTTCGAAGGGAACTACAGAAACACTTGCCAGCTTCTAAATTATGATGAGCTCTCCAGCCAATGTTTTTGTTCCTTTCTTTTCCTTATTACTAATTGGC

Coding sequence (CDS)

ATGGCACCGCTCTTAAACGATCATTTGATCAGTTTTGAAACGCCAGAAAATGAGGCTGTTATCACTAAAAAAATTCCTCGATCTGAACGCACCAACAAATATCCTAATTGGTATTCACCAGATACAGGAATTTGTCATAGTGTCCATGGCTACAGAAAACTTCCCTCTGATCCATTTCTTGATGTTGTCTCCTTCATCTTCTCCTTCCAGTTCCAACACAATGGACATTCAGCTCTCATTGATTCCTCAACTGGAAATTCAATATCTTACAAAGAATTGTTTCCTATGGTAAAATCCATGGCTTCTGGTCTCCATAACTTAGGTATATCACAAGGGGATGTAGTTTTGCTTATGTTACCAAATTCAATTTTCTATCCCATTATTATTTTGGGTGCCCTGTATTTGGGAGCTGTTATTACAACCATGTTTCCTCAAAGCAGTTCCTCTGAAATCAAGAAACGAATCACTGATTGCAATGTGCGTTTGGCTTTTACAATAACTCAGAAAATCAAGAATTTTGAAGCATTGGGAATTAAGACAATTGGTGTACCAGAAAATACGGACTTCGACTTGATGAAGCCTATGGGATTTTCTTCTTTTTATGAACTTATTTCAGGTGGTTCTGATTTGATTAAAAAGCCAGTGATCAGACAAGAAGATACAGCTGCTATATTGTTTTCTTCAGGTACTACTGGAGTGAGCAAAGGGGTTATGTTATCACATAGAAATTTTATATCTACGATCGAGCTTTTCGTTCGGTTTGAAGCTTCACAGTATGAATATTTGCCTACAAAGAATGTGTATTTGGCTGCTGTACCAATGTTTCATATCTATGGTCTCTCCATTTTTGTGATGGGATTAATGTCATTGGGGTCTAGTGTTGTTGTAATGAGCAAATTTGATGTCAAAGATGTGGTCAAGGCCATTGATAGATTTAAGGTCACACATTTTCCAGTTGTTCCACCTATATTGACAGCTTTGACTAGGACTGCAGAGAAAATTGGCGTGCAAAGATTTCGAAGTTTGAAACAAGTTTCTTGTGGGGCTGCTTCTTCAAGCAAGAAGACCATAGATGATTTTGTGCACGCTCTCCCTCATGTTGACTTCATTCAGGGATATGGCATGACAGAGTCCACAGCTATAGGAACCCGTGGTTTCAATACCAAAAATAATGCTAGAAATTATTTGTCAGTGGGACTCTTAGCTCCAAACACAGAAGCTAGAGTCGTCGATTTGGTTGCTGGATCTTGCATGCCTCCTGGTGAAACCGGCGAACTTTTGTTACGTGGACCTGGTTCAATGAAAGGGTACTTGAATAATCCAGAGGCAACAATGTCCACAATAGACCAAGAAAATTGGCTACATACTGGAGACATTGTCTACTTTGACCGAGATGGATACTTGTATGTTGTTGATCGTTTGAAGGAGGTTATAAAGTATAAAGGCTTTCAGGTTGCACCTACTGATTTAGAAGCTGTAGTGATCACACATCCCGAAGTTCTTGATACTGCAGTAGCAGCGGCCAAAGATGAAGAATGTGGTGAAATTCCAGTGGCATTTGTGGTAAAGAAGCCAGGAAGTGCACTCTCACAAAAAGATGTTGTTGATTATGTTGCTCAACAGGTTGCACCATATAAAAGAGTTAGAAAAGTGATTTTTACGGAATCAATACCAAAGTCTGCTGCTGGGAAAGTACTTCGAAGGGAACTACAGAAACACTTGCCAGCTTCTAAATTATGA

Protein sequence

MAPLLNDHLISFETPENEAVITKKIPRSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFLDVVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLPNSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKTIGVPENTDFDLMKPMGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPILTALTRTAEKIGVQRFRSLKQVSCGAASSSKKTIDDFVHALPHVDFIQGYGMTESTAIGTRGFNTKNNARNYLSVGLLAPNTEARVVDLVAGSCMPPGETGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQVAPTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQQVAPYKRVRKVIFTESIPKSAAGKVLRRELQKHLPASKL

Homology

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BLAST of PI0001531 vs. ExPASy Swiss-Prot
Match: Q84P24 (4-coumarate--CoA ligase-like 6 OS=Arabidopsis thaliana OX=3702 GN=4CLL6 PE=1 SV=2)

HSP 1 Score: 636.0 bits (1639), Expect = 4.3e-181
Identity = 320/554 (57.76%), Postives = 429/554 (77.44%), Query Frame = 0


Query: 26  PRSERTNKYPN-WYSPDTGICHSVHGYRKLPSDPFLDVVSFIFSFQFQHNGHSALIDSST 85
           P+++ +++ P  W+S  TGI  S      LP DP LD VS +FS   +H+G +ALIDS T
Sbjct: 12  PKTQTSHQNPPFWFSSKTGIYTSKFPSLHLPVDPNLDAVSALFS--HKHHGDTALIDSLT 71

Query: 86  GNSISYKELFPMVKSMASGL-HNLGISQGDVVLLMLPNSIFYPIIILGALYLGAVITTMF 145
           G SIS+ EL  MV+SMA+G+ H LG+ QGDVV L+LPNS+++P+I L  + LGA++TTM 
Sbjct: 72  GFSISHTELQIMVQSMAAGIYHVLGVRQGDVVSLVLPNSVYFPMIFLSLISLGAIVTTMN 131

Query: 146 PQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKTIGVPENTDFDLMKPMGFSSFYEL 205
           P SS  EIKK++++C+V LAFT T+ ++   +LG+  I V E+ DFD ++ +    FY +
Sbjct: 132 PSSSLGEIKKQVSECSVGLAFTSTENVEKLSSLGVSVISVSESYDFDSIR-IENPKFYSI 191

Query: 206 ISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFVRFEASQYEYLP 265
           +      + KP+I+Q+D AAI++SSGTTG SKGV+L+HRN I+++ELFVRFEASQYEY  
Sbjct: 192 MKESFGFVPKPLIKQDDVAAIMYSSGTTGASKGVLLTHRNLIASMELFVRFEASQYEYPG 251

Query: 266 TKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPI 325
           + NVYLAA+P+ HIYGLS+FVMGL+SLGS++VVM +FD  DVV  I+RFK+THFPVVPP+
Sbjct: 252 SSNVYLAALPLCHIYGLSLFVMGLLSLGSTIVVMKRFDASDVVNVIERFKITHFPVVPPM 311

Query: 326 LTALTRTAEKIGVQRFRSLKQVSCGAASSSKKTIDDFVHALPHVDFIQGYGMTESTAIGT 385
           L ALT+ A+ +  + F+SLKQVS GAA  S+K I+DF+  LPHVD IQGYGMTESTA+GT
Sbjct: 312 LMALTKKAKGVCGEVFKSLKQVSSGAAPLSRKFIEDFLQTLPHVDLIQGYGMTESTAVGT 371

Query: 386 RGFNTKNNARNYLSVGLLAPNTEARVVDLVAGSCMPPGETGELLLRGPGSMKGYLNNPEA 445
           RGFN++  +R Y SVGLLAPN +A+VVD  +GS +PPG  GEL ++GPG MKGYLNNP+A
Sbjct: 372 RGFNSEKLSR-YSSVGLLAPNMQAKVVDWSSGSFLPPGNRGELWIQGPGVMKGYLNNPKA 431

Query: 446 TMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQVAPTDLEAVVITHPEVLDT 505
           T  +I +++WL TGDI YFD DGYL++VDR+KE+IKYKGFQ+AP DLEAV+++HP ++D 
Sbjct: 432 TQMSIVEDSWLRTGDIAYFDEDGYLFIVDRIKEIIKYKGFQIAPADLEAVLVSHPLIIDA 491

Query: 506 AVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQQVAPYKRVRKVIFTESIPKSAAG 565
           AV AA +EECGEIPVAFVV++  + LS++DV+ YVA QVAPY++VRKV+   SIPKS  G
Sbjct: 492 AVTAAPNEECGEIPVAFVVRRQETTLSEEDVISYVASQVAPYRKVRKVVMVNSIPKSPTG 551

Query: 566 KVLRRELQKHLPAS 578
           K+LR+EL++ L  S
Sbjct: 552 KILRKELKRILTNS 561

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BLAST of PI0001531 vs. ExPASy Swiss-Prot
Match: Q6YYZ2 (4-coumarate--CoA ligase-like 3 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL3 PE=3 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 3.8e-145
Identity = 285/551 (51.72%), Postives = 370/551 (67.15%), Query Frame = 0


Query: 35  PNWYSPDTGICHSVHGYRKLPSDPFLDVVSFIFS-FQFQHNGHSALIDSSTGNSISYKEL 94
           P +YS  TGI  S+H    LP+DP L +V+ +F+       G   L+D++T +++S  +L
Sbjct: 25  PPFYSAATGIYSSIHPPVALPTDPSLTLVAHLFARLPLADPGAPTLVDAATASAVSRADL 84

Query: 95  FPMVKSMASGLHNL-GISQGDVVLLMLPNSIFYPIIILGALYLGAVITTMFPQSSSSEIK 154
             +V S+A+GL    G+ +G VVLL+LPNS+ +P+  L  L  GAV TTM P SS +EI 
Sbjct: 85  RRLVASLAAGLRRRHGVRKGSVVLLLLPNSVAFPVSFLAVLAAGAVATTMNPSSSPAEIA 144

Query: 155 KRITDCNVRLAFTITQKIKNFEALGIKTIGVPENTDFDLMKPMG------FSSFYELISG 214
            +       L             L    + VPE  D       G      F++F  ++ G
Sbjct: 145 AQARATGACLVLASRDGAARLPPLAAPVVLVPEILDHSAAADDGDDDQRVFAAFRAMLDG 204

Query: 215 G---SDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFVRFEASQYEYLP 274
           G         PV+ Q+D  AIL+SSGT+G SKGV+L+HRN I+  ELFVRFEASQY    
Sbjct: 205 GGGDGTETAVPVVGQDDAVAILYSSGTSGRSKGVVLTHRNLIAMTELFVRFEASQYHARG 264

Query: 275 TK-NVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPP 334
            + NVY+AA+PM H+YGLS+F +GL+S+G++VVVM +FD  D V AI R+KVTH P+VPP
Sbjct: 265 ARENVYMAALPMSHVYGLSLFAVGLLSIGATVVVMRRFDAGDAVAAIGRYKVTHMPLVPP 324

Query: 335 ILTALTRTAEKIGV--QRFRSLKQVSCGAASSSKKTIDDFVHALPHVDFIQGYGMTESTA 394
           I+ A+ R A   GV   +  SL QVSCGAA  +   I +F+ A PHVDFIQGYGMTESTA
Sbjct: 325 IMAAMVRAAAAGGVPPSQVASLVQVSCGAAPITAALIHEFLQAFPHVDFIQGYGMTESTA 384

Query: 395 IGTRGFNTKNNARNYLSVGLLAPNTEARVVDLVAGSCMPPGETGELLLRGPGSMKGYLNN 454
           +GTRGFNT  + + Y SVGLLAPN  A++V L + SC+PPG +GEL L GPG MKGYL++
Sbjct: 385 VGTRGFNTSKH-KKYTSVGLLAPNMHAKIVHLESSSCLPPGFSGELWLHGPGIMKGYLSD 444

Query: 455 PEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQVAPTDLEAVVITHPEV 514
            +   +   ++ WL TGDI YFD DGYLY+V RLK+ IKYKGFQ+AP DLE V+I HPE+
Sbjct: 445 DDDACTR--KDGWLRTGDIAYFDLDGYLYIVGRLKDTIKYKGFQIAPGDLEEVLIHHPEI 504

Query: 515 LDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQQVAPYKRVRKVIFTESIPKS 572
           LD AV +A+DEE GEIPVAFVV++ GS LS K V++YVA+QVAPYKRVRKV+F E+IPKS
Sbjct: 505 LDVAVTSAEDEEAGEIPVAFVVRRSGSNLSCKQVMEYVAKQVAPYKRVRKVVFVEAIPKS 564

[Close]

BLAST of PI0001531 vs. ExPASy Swiss-Prot
Match: Q336M7 (4-coumarate--CoA ligase-like 2 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL2 PE=3 SV=3)

HSP 1 Score: 463.8 bits (1192), Expect = 2.9e-129
Identity = 262/532 (49.25%), Postives = 348/532 (65.41%), Query Frame = 0


Query: 36  NWYSPDTGICHSVHGYRKLPSDPFLDVVSFIFSFQFQHNGHSALIDSSTGNSISYKELFP 95
           ++YS  TG+  S+H    LPSDP L +V  +FS    H+ HS L+D+ T  ++S  +   
Sbjct: 14  SFYSAATGLYSSLHPPLPLPSDPSLSLVPHLFSHLPLHH-HSLLVDAPTAATLSCADFRR 73

Query: 96  MVKSMASGL-HNLGISQGDVVLLMLPNSIFYPIIILGALYLGAVITTMFPQSSSSEIKKR 155
           +V S+A+GL   L I++G +VLL+LPNS+ +P+  L  L  GAV TTM P S+ +EI  R
Sbjct: 74  LVSSLAAGLRRRLHIARGSLVLLLLPNSLTFPVAFLAVLATGAVATTMNPSSAPAEIAAR 133

Query: 156 ITDCNVRLAFTITQKIKNFEALGIKTIGVPENTDFDLMKPMGFSSFYELISGGSDL-IKK 215
           + D    L    T        L    + VP+              F+ L+    +  ++ 
Sbjct: 134 LRDTAPSLVLASTHNAAKLPPLAAPLVLVPDTFQQQHDDDQFDFFFHALLETDPETPVEM 193

Query: 216 PV-IRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFVRFEASQYEYLPTKNVYLAAV 275
            V + Q+D AA+L+SSGT+G SKGV+++HRN I+ +ELFVRFEASQY      NVYLAA+
Sbjct: 194 GVGVGQDDAAAVLYSSGTSGRSKGVVVTHRNLIAMVELFVRFEASQYTRPARDNVYLAAL 253

Query: 276 PMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPILTALTRTAE 335
           PMFH+YGLS+F +GL+SLG +VVVM +F+V D VKAI ++KVTH P+VPPI++AL R   
Sbjct: 254 PMFHVYGLSLFAVGLLSLGCTVVVMRRFNVDDAVKAIRKYKVTHLPLVPPIMSALLRANP 313

Query: 336 KIGVQRFRSLKQVSCGAASSSKKTIDDFVHALPHVDFIQGYGMTESTAIGTRGFNTKNNA 395
            + +    SL QVS GAA  +   I  F+HA PHVDFIQGYGMTESTA+GTRGFNT  + 
Sbjct: 314 PLELD---SLLQVSSGAAPLNHTLIHHFLHAFPHVDFIQGYGMTESTAVGTRGFNTCKH- 373

Query: 396 RNYLSVGLLAPNTEARVVDLVAGSCMPPGETGELLLRGPGSMK------GYLNNPEATMS 455
           + Y SVGLLAPN  A++V L +GSC+PPG  GEL L GP  MK      GYLN+ +   +
Sbjct: 374 KKYASVGLLAPNMHAKIVHLESGSCLPPGSYGELWLHGPAIMKEFCFVTGYLNDDDDAFT 433

Query: 456 TIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQVAPTDLEAVVITHPEVLDTAVA 515
              ++ WL TGDI YFD DGYL++V RLK+ IKYKGFQ+AP DLEAV+I HPE++D AV 
Sbjct: 434 R--KDGWLRTGDIAYFDSDGYLFIVGRLKDTIKYKGFQIAPADLEAVLIRHPEIIDVAVT 493

Query: 516 AAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQQVAPYKRVRKVIFTESIP 559
           + +DEE GEIPVAFVV+K GS LS   V++YVA+QV    R R+   T+  P
Sbjct: 494 SDEDEEAGEIPVAFVVRKSGSTLSCTHVMEYVAKQV----RTRRSYTTQHSP 534

[Close]

BLAST of PI0001531 vs. ExPASy Swiss-Prot
Match: M4IQR7 (Probable CoA ligase CCL5 OS=Humulus lupulus OX=3486 GN=CCL5 PE=2 SV=1)

HSP 1 Score: 441.4 bits (1134), Expect = 1.5e-122
Identity = 240/550 (43.64%), Postives = 354/550 (64.36%), Query Frame = 0


Query: 38  YSPDTGICHSVHGYRKLPSDPFLDVVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMV 97
           Y     I +S     +LP +  +DV +FI S    H+G  A ID++TG  +++ +L+  V
Sbjct: 15  YCKSNSIFYSKREPVQLPQNHSVDVTTFISS--RAHHGKIAFIDAATGRHLTFPQLWRAV 74

Query: 98  KSMASGLHNLGISQGDVVLLMLPNSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITD 157
            S+A+ L  +GI +GDV+LL+ PNSI++P++ L  + LGA+ITT  P ++  EI K+ITD
Sbjct: 75  DSVATCLSAMGIRKGDVILLLSPNSIYFPVVCLAVMSLGAIITTTNPLNTPREIAKQITD 134

Query: 158 CNVRLAFTITQKIKNFEALGIKTIGVPENTDFDLMKPMGFSSFYELISGGSDLIKKPV-- 217
               LAFTI Q +       +  + + +     L K +   S     S G  + K+P   
Sbjct: 135 SKPVLAFTIPQLVSKIAGSNLPIVIIDDEVKSSLEKTLNIVS-----SLGEMMRKEPSPN 194

Query: 218 -----IRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFVRFEASQYEYLPTKNVYLA 277
                + QEDTA +L+SSGTTG SKGV+ SH+N I+ ++  +    S++     ++ ++ 
Sbjct: 195 RIGYRVNQEDTATLLYSSGTTGASKGVVSSHKNLIAMVQTIL----SRFGTEDGEHTFIC 254

Query: 278 AVPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPILTALTRT 337
            VPMFHIYGL+ F MGL+S GS++V++SKF++ +++ AI++++ T+ P+VPPIL AL + 
Sbjct: 255 TVPMFHIYGLAAFAMGLLSSGSTIVILSKFEIHEMLSAIEKYRATYLPLVPPILMALLKN 314

Query: 338 AEKIGVQ-RFRSLKQVSCGAASSSKKTIDDFVHALPHVDFIQGYGMTESTAIGTRGFNTK 397
           A  I  +    SL+ V  G A  SK+ I+ FV   P V  +QGYG+TEST IG    +  
Sbjct: 315 ANHIRAKYDLSSLQSVLSGGAPLSKEVIEGFVENYPTVSILQGYGLTESTGIGA-STDCL 374

Query: 398 NNARNYLSVGLLAPNTEARVVDLVAGSCMPPGETGELLLRGPGSMKGYLNNPEATMSTID 457
             +R Y + G+L+P+ EA++V+   G  +    TGEL LRGP  MKGY +N EAT STID
Sbjct: 375 QESRRYGTAGMLSPSMEAKIVNPETGEALSVNRTGELWLRGPTIMKGYFSNEEATSSTID 434

Query: 458 QENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQVAPTDLEAVVITHPEVLDTAVAAAK 517
            E WL TGD+ Y D DG+++VVDRLKE+IKYKG+QVAP +LEA++++HPE+ D AV    
Sbjct: 435 SEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLSHPEISDAAVIPYP 494

Query: 518 DEECGEIPVAFVVKKPGSALSQKDVVDYVAQQVAPYKRVRKVIFTESIPKSAAGKVLRRE 577
           D+E G+ P+A+VV+K GS LS+  V+D++A+ VAPYKR+RKV F  SIPK+ +GK+LR++
Sbjct: 495 DKEAGQFPMAYVVRKGGSNLSESTVMDFIAKLVAPYKRIRKVAFVASIPKNPSGKILRKD 551

Query: 578 LQKHLPASKL 580
           L K L  SKL
Sbjct: 555 LIK-LATSKL 551

[Close]

BLAST of PI0001531 vs. ExPASy Swiss-Prot
Match: Q84P21 (4-coumarate--CoA ligase-like 5 OS=Arabidopsis thaliana OX=3702 GN=4CLL5 PE=1 SV=2)

HSP 1 Score: 438.0 bits (1125), Expect = 1.7e-121
Identity = 243/550 (44.18%), Postives = 351/550 (63.82%), Query Frame = 0


Query: 27  RSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFLDVVSFIFSFQFQHNGHSALIDSSTGN 86
           RS   N    +YS  T I         LP +P LDV +FI S    H G  A ID+STG 
Sbjct: 7   RSGFCNSNSTFYSKRTPI--------PLPPNPSLDVTTFISS--QAHRGRIAFIDASTGQ 66

Query: 87  SISYKELFPMVKSMASGLHNLGISQGDVVLLMLPNSIFYPIIILGALYLGAVITTMFPQS 146
           ++++ EL+  V+S+A  L  +GI +G VVLL+ PNSI +P++ L  + LGA+ITT  P +
Sbjct: 67  NLTFTELWRAVESVADCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLN 126

Query: 147 SSSEIKKRITDCNVRLAFTITQKIKNFEALGIK--TIGVPENTDFDLMKPMGFSSFYELI 206
           +S+EI K+I D N  LAFT +Q +    A   K   + + E     +          +  
Sbjct: 127 TSNEIAKQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGDVRRLVEMMKKE 186

Query: 207 SGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFV-RFEASQYEYLP 266
             G+ +  K  + Q+DTA +L+SSGTTG+SKGV+ SHRN I+ ++  V RF +   E   
Sbjct: 187 PSGNRV--KERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDDGE--- 246

Query: 267 TKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPI 326
               ++  VPMFHIYGL+ F  GL++ GS+++V+SKF++ +++ AI +++ T  P+VPPI
Sbjct: 247 --QRFICTVPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPI 306

Query: 327 LTALTRTAEKIGVQ-RFRSLKQVSCGAASSSKKTIDDFVHALPHVDFIQGYGMTESTAIG 386
           L A+   A++I  +    S+  V CG A  SK+  + F    P V  +QGYG+TEST IG
Sbjct: 307 LVAMVNGADQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTGIG 366

Query: 387 TRGFNTKNNARNYLSVGLLAPNTEARVVDLVAGSCMPPGETGELLLRGPGSMKGYLNNPE 446
               +T   +R Y + G L+ + E R+VD V G  + P +TGEL L+GP  MKGY +N E
Sbjct: 367 A-STDTVEESRRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNEE 426

Query: 447 ATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQVAPTDLEAVVITHPEVLD 506
           AT ST+D E WL TGD+ Y D DG+++VVDRLKE+IKYKG+QVAP +LEA+++THPE+ D
Sbjct: 427 ATSSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITD 486

Query: 507 TAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQQVAPYKRVRKVIFTESIPKSAA 566
            AV    D+E G+ P+A+VV+K GS+LS+K ++++VA+QVAPYKR+RKV F  SIPK+ +
Sbjct: 487 AAVIPFPDKEVGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIRKVAFVSSIPKNPS 538

Query: 567 GKVLRRELQK 573
           GK+LR++L K
Sbjct: 547 GKILRKDLIK 538

[Close]

BLAST of PI0001531 vs. ExPASy TrEMBL
Match: A0A0A0L8X1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G638510 PE=4 SV=1)

HSP 1 Score: 1074.7 bits (2778), Expect = 2.2e-310
Identity = 547/579 (94.47%), Postives = 563/579 (97.24%), Query Frame = 0


Query: 1   MAPLLNDHLISFETPENEAVITKKIPRSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60
           MA L+N+   SFETPENEAVITKKIPRSERTNKYPNWYSPDTGIC SVHGYRKLPSDPFL
Sbjct: 1   MARLINNQ-FSFETPENEAVITKKIPRSERTNKYPNWYSPDTGICRSVHGYRKLPSDPFL 60

Query: 61  DVVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120
           DVVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP
Sbjct: 61  DVVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120

Query: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180
           NSIF+PIIILGALYLGAV+TTMFPQSSSSEIKKRITDCNVRLAF ITQKIKNFEALGIKT
Sbjct: 121 NSIFFPIIILGALYLGAVVTTMFPQSSSSEIKKRITDCNVRLAFAITQKIKNFEALGIKT 180

Query: 181 IGVPENTDFDLMKPMGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLS 240
           IGVPENT+F+LM+ MGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLS
Sbjct: 181 IGVPENTNFNLMRSMGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLS 240

Query: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300
           HRNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKF
Sbjct: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300

Query: 301 DVKDVVKAIDRFKVTHFPVVPPILTALTRTAEKIGVQRFRSLKQVSCGAASSSKKTIDDF 360
           DVKDVVKAIDRFKVTHFPVVPPILTAL RTAEKIGV RFRSLKQVSCGAA+SSKKTID+F
Sbjct: 301 DVKDVVKAIDRFKVTHFPVVPPILTALARTAEKIGVHRFRSLKQVSCGAAASSKKTIDNF 360

Query: 361 VHALPHVDFIQGYGMTESTAIGTRGFNTKNNARNYLSVGLLAPNTEARVVDLVAGSCMPP 420
           VHALPHVDFIQGYGMTESTA+GTRGFNTK NARNYLSVGLLAPNTEA+VVD V GS MPP
Sbjct: 361 VHALPHVDFIQGYGMTESTAVGTRGFNTK-NARNYLSVGLLAPNTEAKVVDWVTGSSMPP 420

Query: 421 GETGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKY 480
           G+TGELLLRGPGSMKGYLNNPEAT  T+DQENWLHTGDIVYFDRDGYLYVVDRLKEVIKY
Sbjct: 421 GKTGELLLRGPGSMKGYLNNPEATTFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKY 480

Query: 481 KGFQVAPTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQ 540
           KGFQ+APTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSAL+QKDVVDYVAQ
Sbjct: 481 KGFQIAPTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQ 540

Query: 541 QVAPYKRVRKVIFTESIPKSAAGKVLRRELQKHLPASKL 580
           QVAPYK+VRKVIFTESIPKSAAGKVLRRELQKH PASKL
Sbjct: 541 QVAPYKKVRKVIFTESIPKSAAGKVLRRELQKHFPASKL 577

[Close]

BLAST of PI0001531 vs. ExPASy TrEMBL
Match: A0A5D3DC80 (4-coumarate--CoA ligase-like 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold392G00450 PE=4 SV=1)

HSP 1 Score: 1071.2 bits (2769), Expect = 1.5e-309
Identity = 546/579 (94.30%), Postives = 559/579 (96.55%), Query Frame = 0


Query: 1   MAPLLNDHLISFETPENEAVITKKIPRSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60
           MA LL++   SFETPENEA ITKKIP SERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL
Sbjct: 1   MARLLSNDQFSFETPENEAAITKKIPLSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60

Query: 61  DVVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120
           DVVSFIFS QFQHNGHSALIDS TGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP
Sbjct: 61  DVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120

Query: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180
           NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT
Sbjct: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180

Query: 181 IGVPENTDFDLMKPMGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLS 240
           IGVPENT+FDLM+PMGFSSFYELIS GSDLIK+PVIRQEDTAAILFSSGTTGVSKGVMLS
Sbjct: 181 IGVPENTNFDLMRPMGFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLS 240

Query: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300
           HRNFISTIELFVRFEASQYEYLPTKNVYLAA+PMFHIYGLSIFVMGLMSLGSSVVVMSKF
Sbjct: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300

Query: 301 DVKDVVKAIDRFKVTHFPVVPPILTALTRTAEKIGVQRFRSLKQVSCGAASSSKKTIDDF 360
           DVKDVVKAIDRFKVTHFPVVPPIL AL+RTAEKIGV+RFRSLKQVSCGAA+SSKKTIDDF
Sbjct: 301 DVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDF 360

Query: 361 VHALPHVDFIQGYGMTESTAIGTRGFNTKNNARNYLSVGLLAPNTEARVVDLVAGSCMPP 420
           V AL HVDFIQGYGMTESTAIGTRGFN K   RNYLSVGLLAPNTEA+VVD V GS MPP
Sbjct: 361 VQALSHVDFIQGYGMTESTAIGTRGFNNK-KTRNYLSVGLLAPNTEAKVVDWVTGSSMPP 420

Query: 421 GETGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKY 480
           G+TGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKY
Sbjct: 421 GKTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKY 480

Query: 481 KGFQVAPTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQ 540
           KGFQ+APTDLEAVVI HPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQ
Sbjct: 481 KGFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQ 540

Query: 541 QVAPYKRVRKVIFTESIPKSAAGKVLRRELQKHLPASKL 580
           QVAPYK+VRKVIFTESIPKSAAGKVLRRELQKHLPASKL
Sbjct: 541 QVAPYKKVRKVIFTESIPKSAAGKVLRRELQKHLPASKL 578

[Close]

BLAST of PI0001531 vs. ExPASy TrEMBL
Match: A0A1S3CCH7 (4-coumarate--CoA ligase-like 6 OS=Cucumis melo OX=3656 GN=LOC103499264 PE=4 SV=1)

HSP 1 Score: 1004.6 bits (2596), Expect = 1.7e-289
Identity = 512/553 (92.59%), Postives = 527/553 (95.30%), Query Frame = 0


Query: 1   MAPLLNDHLISFETPENEAVITKKIPRSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60
           MA LL++   SFETPENEA ITKKIP SERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL
Sbjct: 1   MARLLSNDQFSFETPENEAAITKKIPLSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60

Query: 61  DVVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120
           DVVSFIFS QFQHNGHSALIDS TGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP
Sbjct: 61  DVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120

Query: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180
           NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT
Sbjct: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180

Query: 181 IGVPENTDFDLMKPMGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLS 240
           IGVPENT+FDLM+PMGFSSFYELIS GSDLIK+PVIRQEDTAAILFSSGTTGVSKGVMLS
Sbjct: 181 IGVPENTNFDLMRPMGFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLS 240

Query: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300
           HRNFISTIELFVRFEASQYEYLPTKNVYLAA+PMFHIYGLSIFVMGLMSLGSSVVVMSKF
Sbjct: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300

Query: 301 DVKDVVKAIDRFKVTHFPVVPPILTALTRTAEKIGVQRFRSLKQVSCGAASSSKKTIDDF 360
           DVKDVVKAIDRFKVTHFPVVPPIL AL+RTAEKIGV+RFRSLKQVSCGAA+SSKKTIDDF
Sbjct: 301 DVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDF 360

Query: 361 VHALPHVDFIQGYGMTESTAIGTRGFNTKNNARNYLSVGLLAPNTEARVVDLVAGSCMPP 420
           V AL HVDFIQGYGMTESTAIGTRGFN K   RNYLSVGLLAPNTEA+VVD V GS MPP
Sbjct: 361 VQALSHVDFIQGYGMTESTAIGTRGFNNK-KTRNYLSVGLLAPNTEAKVVDWVTGSSMPP 420

Query: 421 GETGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKY 480
           G+TGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKY
Sbjct: 421 GKTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKY 480

Query: 481 KGFQVAPTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQ 540
           KGFQ+APTDLEAVVI HPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQ
Sbjct: 481 KGFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQ 540

Query: 541 QVAPYKRVRKVIF 554
           QV+   ++   IF
Sbjct: 541 QVSHIFQICPCIF 552

[Close]

BLAST of PI0001531 vs. ExPASy TrEMBL
Match: A0A5A7VLT7 (4-coumarate--CoA ligase-like 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold238G001280 PE=4 SV=1)

HSP 1 Score: 1002.3 bits (2590), Expect = 8.4e-289
Identity = 509/541 (94.09%), Postives = 521/541 (96.30%), Query Frame = 0


Query: 1   MAPLLNDHLISFETPENEAVITKKIPRSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60
           MA LL++   SFETPENEA ITKKIP SERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL
Sbjct: 1   MARLLSNDQFSFETPENEAAITKKIPLSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60

Query: 61  DVVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120
           DVVSFIFS QFQHNGHSALIDS TGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP
Sbjct: 61  DVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120

Query: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180
           NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT
Sbjct: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180

Query: 181 IGVPENTDFDLMKPMGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLS 240
           IGVPENT+FDLM+PMGFSSFYELIS GSDLIK+PVIRQEDTAAILFSSGTTGVSKGVMLS
Sbjct: 181 IGVPENTNFDLMRPMGFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLS 240

Query: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300
           HRNFISTIELFVRFEASQYEYLPTKNVYLAA+PMFHIYGLSIFVMGLMSLGSSVVVMSKF
Sbjct: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300

Query: 301 DVKDVVKAIDRFKVTHFPVVPPILTALTRTAEKIGVQRFRSLKQVSCGAASSSKKTIDDF 360
           DVKDVVKAIDRFKVTHFPVVPPIL AL+RTAEKIGV+RFRSLKQVSCGAA+SSKKTIDDF
Sbjct: 301 DVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDF 360

Query: 361 VHALPHVDFIQGYGMTESTAIGTRGFNTKNNARNYLSVGLLAPNTEARVVDLVAGSCMPP 420
           V AL HVDFIQGYGMTESTAIGTRGFN K   RNYLSVGLLAPNTEA+VVD V GS MPP
Sbjct: 361 VQALSHVDFIQGYGMTESTAIGTRGFNNK-KTRNYLSVGLLAPNTEAKVVDWVTGSSMPP 420

Query: 421 GETGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKY 480
           G+TGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKY
Sbjct: 421 GKTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKY 480

Query: 481 KGFQVAPTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQ 540
           KGFQ+APTDLEAVVI HPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQ
Sbjct: 481 KGFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQ 540

Query: 541 Q 542
           Q
Sbjct: 541 Q 540

[Close]

BLAST of PI0001531 vs. ExPASy TrEMBL
Match: A0A6J1HGK9 (4-coumarate--CoA ligase-like 6 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111464107 PE=4 SV=1)

HSP 1 Score: 937.6 bits (2422), Expect = 2.5e-269
Identity = 472/576 (81.94%), Postives = 520/576 (90.28%), Query Frame = 0


Query: 1   MAPLLNDHLISFETPENEAVITKKIPRSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60
           MAPLLND   SF+TPE++ V TKKIP     NKYP WYSPDTGIC SVH  R LP+DPFL
Sbjct: 1   MAPLLNDQ-FSFQTPESQTVQTKKIP-----NKYPGWYSPDTGICRSVHASRDLPTDPFL 60

Query: 61  DVVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120
           DV S+I S  FQHNG SALIDSSTG+SISY+EL+ MV SMASGL  LG+SQGDVVLL+LP
Sbjct: 61  DVASYILS--FQHNGRSALIDSSTGHSISYRELYRMVNSMASGLQKLGVSQGDVVLLLLP 120

Query: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180
           NSIFYPI++LG LYLGAVITTMFPQSSS EIKKRI++CNVRLAF   Q + NFEA G++ 
Sbjct: 121 NSIFYPIVLLGVLYLGAVITTMFPQSSSQEIKKRISECNVRLAFATPQNVGNFEAFGVQA 180

Query: 181 IGVPENTDFDLMKPMGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLS 240
           IGVPENT+ DLM+PMGFSSFYELISGG DL K+PVIRQEDTAAILFSSGTTGVSKGV+L+
Sbjct: 181 IGVPENTNLDLMRPMGFSSFYELISGGFDLNKRPVIRQEDTAAILFSSGTTGVSKGVLLT 240

Query: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300
           HRNFIST+ELFVRFEASQYEYL T+NVYLAA+PMFH+YGLSIFVMGL+SLGSS+VVMSKF
Sbjct: 241 HRNFISTVELFVRFEASQYEYLTTENVYLAAIPMFHVYGLSIFVMGLLSLGSSIVVMSKF 300

Query: 301 DVKDVVKAIDRFKVTHFPVVPPILTALTRTAEKIGVQRFRSLKQVSCGAASSSKKTIDDF 360
           DVK+VV AIDRFKVTHFPVVPPI+  + RTA K G  +FRSLKQVSCGAAS SKK I DF
Sbjct: 301 DVKEVVVAIDRFKVTHFPVVPPIMIVMARTAVKFGGHKFRSLKQVSCGAASLSKKIIGDF 360

Query: 361 VHALPHVDFIQGYGMTESTAIGTRGFNTKNNARNYLSVGLLAPNTEARVVDLVAGSCMPP 420
           V ALPHVDFIQGYGMTE+TA+GTRGFNTK  A+NYLSVGLLAPN EA+VVD V+GS MPP
Sbjct: 361 VQALPHVDFIQGYGMTETTAVGTRGFNTK-KAQNYLSVGLLAPNMEAKVVDWVSGSIMPP 420

Query: 421 GETGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKY 480
           G+TGELLLRGPG MKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKY
Sbjct: 421 GKTGELLLRGPGLMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKY 480

Query: 481 KGFQVAPTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQ 540
           KGFQ+APTDLE+VVITHPEVLDTAVAAA+DEECGEIPVAFVVKKPGS+LSQKDV+DYVAQ
Sbjct: 481 KGFQIAPTDLESVVITHPEVLDTAVAAAEDEECGEIPVAFVVKKPGSSLSQKDVIDYVAQ 540

Query: 541 QVAPYKRVRKVIFTESIPKSAAGKVLRRELQKHLPA 577
           QVAPYK++RKV+FTESIPKSAAGKVLR+EL KHLP+
Sbjct: 541 QVAPYKKIRKVVFTESIPKSAAGKVLRKELGKHLPS 567

[Close]

BLAST of PI0001531 vs. NCBI nr
Match: XP_004144410.1 (4-coumarate--CoA ligase-like 6 [Cucumis sativus] >KGN58405.1 hypothetical protein Csa_001031 [Cucumis sativus])

HSP 1 Score: 1074.7 bits (2778), Expect = 4.4e-310
Identity = 547/579 (94.47%), Postives = 563/579 (97.24%), Query Frame = 0


Query: 1   MAPLLNDHLISFETPENEAVITKKIPRSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60
           MA L+N+   SFETPENEAVITKKIPRSERTNKYPNWYSPDTGIC SVHGYRKLPSDPFL
Sbjct: 1   MARLINNQ-FSFETPENEAVITKKIPRSERTNKYPNWYSPDTGICRSVHGYRKLPSDPFL 60

Query: 61  DVVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120
           DVVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP
Sbjct: 61  DVVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120

Query: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180
           NSIF+PIIILGALYLGAV+TTMFPQSSSSEIKKRITDCNVRLAF ITQKIKNFEALGIKT
Sbjct: 121 NSIFFPIIILGALYLGAVVTTMFPQSSSSEIKKRITDCNVRLAFAITQKIKNFEALGIKT 180

Query: 181 IGVPENTDFDLMKPMGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLS 240
           IGVPENT+F+LM+ MGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLS
Sbjct: 181 IGVPENTNFNLMRSMGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLS 240

Query: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300
           HRNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKF
Sbjct: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300

Query: 301 DVKDVVKAIDRFKVTHFPVVPPILTALTRTAEKIGVQRFRSLKQVSCGAASSSKKTIDDF 360
           DVKDVVKAIDRFKVTHFPVVPPILTAL RTAEKIGV RFRSLKQVSCGAA+SSKKTID+F
Sbjct: 301 DVKDVVKAIDRFKVTHFPVVPPILTALARTAEKIGVHRFRSLKQVSCGAAASSKKTIDNF 360

Query: 361 VHALPHVDFIQGYGMTESTAIGTRGFNTKNNARNYLSVGLLAPNTEARVVDLVAGSCMPP 420
           VHALPHVDFIQGYGMTESTA+GTRGFNTK NARNYLSVGLLAPNTEA+VVD V GS MPP
Sbjct: 361 VHALPHVDFIQGYGMTESTAVGTRGFNTK-NARNYLSVGLLAPNTEAKVVDWVTGSSMPP 420

Query: 421 GETGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKY 480
           G+TGELLLRGPGSMKGYLNNPEAT  T+DQENWLHTGDIVYFDRDGYLYVVDRLKEVIKY
Sbjct: 421 GKTGELLLRGPGSMKGYLNNPEATTFTMDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKY 480

Query: 481 KGFQVAPTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQ 540
           KGFQ+APTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSAL+QKDVVDYVAQ
Sbjct: 481 KGFQIAPTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALTQKDVVDYVAQ 540

Query: 541 QVAPYKRVRKVIFTESIPKSAAGKVLRRELQKHLPASKL 580
           QVAPYK+VRKVIFTESIPKSAAGKVLRRELQKH PASKL
Sbjct: 541 QVAPYKKVRKVIFTESIPKSAAGKVLRRELQKHFPASKL 577

[Close]

BLAST of PI0001531 vs. NCBI nr
Match: TYK21080.1 (4-coumarate--CoA ligase-like 6 [Cucumis melo var. makuwa])

HSP 1 Score: 1071.2 bits (2769), Expect = 3.1e-309
Identity = 546/579 (94.30%), Postives = 559/579 (96.55%), Query Frame = 0


Query: 1   MAPLLNDHLISFETPENEAVITKKIPRSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60
           MA LL++   SFETPENEA ITKKIP SERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL
Sbjct: 1   MARLLSNDQFSFETPENEAAITKKIPLSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60

Query: 61  DVVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120
           DVVSFIFS QFQHNGHSALIDS TGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP
Sbjct: 61  DVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120

Query: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180
           NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT
Sbjct: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180

Query: 181 IGVPENTDFDLMKPMGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLS 240
           IGVPENT+FDLM+PMGFSSFYELIS GSDLIK+PVIRQEDTAAILFSSGTTGVSKGVMLS
Sbjct: 181 IGVPENTNFDLMRPMGFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLS 240

Query: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300
           HRNFISTIELFVRFEASQYEYLPTKNVYLAA+PMFHIYGLSIFVMGLMSLGSSVVVMSKF
Sbjct: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300

Query: 301 DVKDVVKAIDRFKVTHFPVVPPILTALTRTAEKIGVQRFRSLKQVSCGAASSSKKTIDDF 360
           DVKDVVKAIDRFKVTHFPVVPPIL AL+RTAEKIGV+RFRSLKQVSCGAA+SSKKTIDDF
Sbjct: 301 DVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDF 360

Query: 361 VHALPHVDFIQGYGMTESTAIGTRGFNTKNNARNYLSVGLLAPNTEARVVDLVAGSCMPP 420
           V AL HVDFIQGYGMTESTAIGTRGFN K   RNYLSVGLLAPNTEA+VVD V GS MPP
Sbjct: 361 VQALSHVDFIQGYGMTESTAIGTRGFNNK-KTRNYLSVGLLAPNTEAKVVDWVTGSSMPP 420

Query: 421 GETGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKY 480
           G+TGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKY
Sbjct: 421 GKTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKY 480

Query: 481 KGFQVAPTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQ 540
           KGFQ+APTDLEAVVI HPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQ
Sbjct: 481 KGFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQ 540

Query: 541 QVAPYKRVRKVIFTESIPKSAAGKVLRRELQKHLPASKL 580
           QVAPYK+VRKVIFTESIPKSAAGKVLRRELQKHLPASKL
Sbjct: 541 QVAPYKKVRKVIFTESIPKSAAGKVLRRELQKHLPASKL 578

[Close]

BLAST of PI0001531 vs. NCBI nr
Match: XP_008460452.1 (PREDICTED: 4-coumarate--CoA ligase-like 6 [Cucumis melo])

HSP 1 Score: 1004.6 bits (2596), Expect = 3.5e-289
Identity = 512/553 (92.59%), Postives = 527/553 (95.30%), Query Frame = 0


Query: 1   MAPLLNDHLISFETPENEAVITKKIPRSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60
           MA LL++   SFETPENEA ITKKIP SERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL
Sbjct: 1   MARLLSNDQFSFETPENEAAITKKIPLSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60

Query: 61  DVVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120
           DVVSFIFS QFQHNGHSALIDS TGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP
Sbjct: 61  DVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120

Query: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180
           NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT
Sbjct: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180

Query: 181 IGVPENTDFDLMKPMGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLS 240
           IGVPENT+FDLM+PMGFSSFYELIS GSDLIK+PVIRQEDTAAILFSSGTTGVSKGVMLS
Sbjct: 181 IGVPENTNFDLMRPMGFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLS 240

Query: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300
           HRNFISTIELFVRFEASQYEYLPTKNVYLAA+PMFHIYGLSIFVMGLMSLGSSVVVMSKF
Sbjct: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300

Query: 301 DVKDVVKAIDRFKVTHFPVVPPILTALTRTAEKIGVQRFRSLKQVSCGAASSSKKTIDDF 360
           DVKDVVKAIDRFKVTHFPVVPPIL AL+RTAEKIGV+RFRSLKQVSCGAA+SSKKTIDDF
Sbjct: 301 DVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDF 360

Query: 361 VHALPHVDFIQGYGMTESTAIGTRGFNTKNNARNYLSVGLLAPNTEARVVDLVAGSCMPP 420
           V AL HVDFIQGYGMTESTAIGTRGFN K   RNYLSVGLLAPNTEA+VVD V GS MPP
Sbjct: 361 VQALSHVDFIQGYGMTESTAIGTRGFNNK-KTRNYLSVGLLAPNTEAKVVDWVTGSSMPP 420

Query: 421 GETGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKY 480
           G+TGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKY
Sbjct: 421 GKTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKY 480

Query: 481 KGFQVAPTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQ 540
           KGFQ+APTDLEAVVI HPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQ
Sbjct: 481 KGFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQ 540

Query: 541 QVAPYKRVRKVIF 554
           QV+   ++   IF
Sbjct: 541 QVSHIFQICPCIF 552

[Close]

BLAST of PI0001531 vs. NCBI nr
Match: KAA0068160.1 (4-coumarate--CoA ligase-like 6 [Cucumis melo var. makuwa])

HSP 1 Score: 1002.3 bits (2590), Expect = 1.7e-288
Identity = 509/541 (94.09%), Postives = 521/541 (96.30%), Query Frame = 0


Query: 1   MAPLLNDHLISFETPENEAVITKKIPRSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60
           MA LL++   SFETPENEA ITKKIP SERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL
Sbjct: 1   MARLLSNDQFSFETPENEAAITKKIPLSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60

Query: 61  DVVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120
           DVVSFIFS QFQHNGHSALIDS TGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP
Sbjct: 61  DVVSFIFSVQFQHNGHSALIDSLTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120

Query: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180
           NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT
Sbjct: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180

Query: 181 IGVPENTDFDLMKPMGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLS 240
           IGVPENT+FDLM+PMGFSSFYELIS GSDLIK+PVIRQEDTAAILFSSGTTGVSKGVMLS
Sbjct: 181 IGVPENTNFDLMRPMGFSSFYELISSGSDLIKRPVIRQEDTAAILFSSGTTGVSKGVMLS 240

Query: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300
           HRNFISTIELFVRFEASQYEYLPTKNVYLAA+PMFHIYGLSIFVMGLMSLGSSVVVMSKF
Sbjct: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAIPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300

Query: 301 DVKDVVKAIDRFKVTHFPVVPPILTALTRTAEKIGVQRFRSLKQVSCGAASSSKKTIDDF 360
           DVKDVVKAIDRFKVTHFPVVPPIL AL+RTAEKIGV+RFRSLKQVSCGAA+SSKKTIDDF
Sbjct: 301 DVKDVVKAIDRFKVTHFPVVPPILIALSRTAEKIGVRRFRSLKQVSCGAAASSKKTIDDF 360

Query: 361 VHALPHVDFIQGYGMTESTAIGTRGFNTKNNARNYLSVGLLAPNTEARVVDLVAGSCMPP 420
           V AL HVDFIQGYGMTESTAIGTRGFN K   RNYLSVGLLAPNTEA+VVD V GS MPP
Sbjct: 361 VQALSHVDFIQGYGMTESTAIGTRGFNNK-KTRNYLSVGLLAPNTEAKVVDWVTGSSMPP 420

Query: 421 GETGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKY 480
           G+TGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKY
Sbjct: 421 GKTGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKY 480

Query: 481 KGFQVAPTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQ 540
           KGFQ+APTDLEAVVI HPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQ
Sbjct: 481 KGFQIAPTDLEAVVIAHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQ 540

Query: 541 Q 542
           Q
Sbjct: 541 Q 540

[Close]

BLAST of PI0001531 vs. NCBI nr
Match: XP_038888724.1 (4-coumarate--CoA ligase-like 6 [Benincasa hispida])

HSP 1 Score: 1000.7 bits (2586), Expect = 5.0e-288
Identity = 504/576 (87.50%), Postives = 544/576 (94.44%), Query Frame = 0


Query: 1   MAPLLNDHLISFETPENEAVITKKIPRSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFL 60
           MAPLLNDH  S++TPENE VITKKIPRSE+TNKYPNWYSPDTGIC SVH  R +PSDPFL
Sbjct: 1   MAPLLNDH-FSYQTPENETVITKKIPRSEQTNKYPNWYSPDTGICRSVHASRGVPSDPFL 60

Query: 61  DVVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMASGLHNLGISQGDVVLLMLP 120
           DVVSFIFS  F+HNG+SA+IDSSTG+SISYKE+F MVKSMASGL   GISQGDVVLL+LP
Sbjct: 61  DVVSFIFS--FKHNGNSAIIDSSTGSSISYKEMFHMVKSMASGLQKFGISQGDVVLLVLP 120

Query: 121 NSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKT 180
           NSIFYPII+LG LYLGAV+TTMFPQSSSSEIKKRI DCNVRLAFTITQKIKNFE+LGI+ 
Sbjct: 121 NSIFYPIILLGVLYLGAVVTTMFPQSSSSEIKKRINDCNVRLAFTITQKIKNFESLGIQA 180

Query: 181 IGVPENTDFDLMKPMGFSSFYELISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLS 240
           IGVPE T+FDLM+PMGFS F+ELIS GSDL ++PVIRQEDTAA+LFSSGTTGVSKGVML+
Sbjct: 181 IGVPEITNFDLMQPMGFSYFHELISSGSDLKQRPVIRQEDTAALLFSSGTTGVSKGVMLT 240

Query: 241 HRNFISTIELFVRFEASQYEYLPTKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKF 300
           HRNFISTIELFVRFEASQYEYLPT+NVYLAA+PMFHIYGLSIFVMGL+SLGSSVV+MSKF
Sbjct: 241 HRNFISTIELFVRFEASQYEYLPTENVYLAAIPMFHIYGLSIFVMGLLSLGSSVVIMSKF 300

Query: 301 DVKDVVKAIDRFKVTHFPVVPPILTALTRTAEKIGVQRFRSLKQVSCGAASSSKKTIDDF 360
           DVK+VVKAIDRFKVTHFPVVPPI+TA+ RTAEKIGV RFRSLKQVSCGAAS +KKTIDDF
Sbjct: 301 DVKEVVKAIDRFKVTHFPVVPPIMTAMARTAEKIGVHRFRSLKQVSCGAASLNKKTIDDF 360

Query: 361 VHALPHVDFIQGYGMTESTAIGTRGFNTKNNARNYLSVGLLAPNTEARVVDLVAGSCMPP 420
           V A PHVDFIQGYGMTESTAIGTRGFNTK NARNY SVGLLAPNTEA+VVD V+GS MPP
Sbjct: 361 VKAFPHVDFIQGYGMTESTAIGTRGFNTK-NARNYTSVGLLAPNTEAKVVDWVSGSFMPP 420

Query: 421 GETGELLLRGPGSMKGYLNNPEATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKY 480
           G+TGELLLRGPGSMKGYLNNPEATMSTID+ENWLHTGDIVYFDRDGYLYV+DRLKEVIKY
Sbjct: 421 GKTGELLLRGPGSMKGYLNNPEATMSTIDKENWLHTGDIVYFDRDGYLYVIDRLKEVIKY 480

Query: 481 KGFQVAPTDLEAVVITHPEVLDTAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQ 540
           KGFQ+APTDLEAVV+THPEVLDTAV AAKDEECGEIPVAFVVKKPGS LSQKDV+DYVAQ
Sbjct: 481 KGFQIAPTDLEAVVMTHPEVLDTAVTAAKDEECGEIPVAFVVKKPGSKLSQKDVIDYVAQ 540

Query: 541 QVAPYKRVRKVIFTESIPKSAAGKVLRRELQKHLPA 577
           QVAPYK+VRKV+FTESIPKSAAGKVLRREL KHLP+
Sbjct: 541 QVAPYKKVRKVVFTESIPKSAAGKVLRRELGKHLPS 572

[Close]

BLAST of PI0001531 vs. TAIR 10
Match: AT4G19010.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 636.0 bits (1639), Expect = 3.0e-182
Identity = 320/554 (57.76%), Postives = 429/554 (77.44%), Query Frame = 0


Query: 26  PRSERTNKYPN-WYSPDTGICHSVHGYRKLPSDPFLDVVSFIFSFQFQHNGHSALIDSST 85
           P+++ +++ P  W+S  TGI  S      LP DP LD VS +FS   +H+G +ALIDS T
Sbjct: 12  PKTQTSHQNPPFWFSSKTGIYTSKFPSLHLPVDPNLDAVSALFS--HKHHGDTALIDSLT 71

Query: 86  GNSISYKELFPMVKSMASGL-HNLGISQGDVVLLMLPNSIFYPIIILGALYLGAVITTMF 145
           G SIS+ EL  MV+SMA+G+ H LG+ QGDVV L+LPNS+++P+I L  + LGA++TTM 
Sbjct: 72  GFSISHTELQIMVQSMAAGIYHVLGVRQGDVVSLVLPNSVYFPMIFLSLISLGAIVTTMN 131

Query: 146 PQSSSSEIKKRITDCNVRLAFTITQKIKNFEALGIKTIGVPENTDFDLMKPMGFSSFYEL 205
           P SS  EIKK++++C+V LAFT T+ ++   +LG+  I V E+ DFD ++ +    FY +
Sbjct: 132 PSSSLGEIKKQVSECSVGLAFTSTENVEKLSSLGVSVISVSESYDFDSIR-IENPKFYSI 191

Query: 206 ISGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFVRFEASQYEYLP 265
           +      + KP+I+Q+D AAI++SSGTTG SKGV+L+HRN I+++ELFVRFEASQYEY  
Sbjct: 192 MKESFGFVPKPLIKQDDVAAIMYSSGTTGASKGVLLTHRNLIASMELFVRFEASQYEYPG 251

Query: 266 TKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPI 325
           + NVYLAA+P+ HIYGLS+FVMGL+SLGS++VVM +FD  DVV  I+RFK+THFPVVPP+
Sbjct: 252 SSNVYLAALPLCHIYGLSLFVMGLLSLGSTIVVMKRFDASDVVNVIERFKITHFPVVPPM 311

Query: 326 LTALTRTAEKIGVQRFRSLKQVSCGAASSSKKTIDDFVHALPHVDFIQGYGMTESTAIGT 385
           L ALT+ A+ +  + F+SLKQVS GAA  S+K I+DF+  LPHVD IQGYGMTESTA+GT
Sbjct: 312 LMALTKKAKGVCGEVFKSLKQVSSGAAPLSRKFIEDFLQTLPHVDLIQGYGMTESTAVGT 371

Query: 386 RGFNTKNNARNYLSVGLLAPNTEARVVDLVAGSCMPPGETGELLLRGPGSMKGYLNNPEA 445
           RGFN++  +R Y SVGLLAPN +A+VVD  +GS +PPG  GEL ++GPG MKGYLNNP+A
Sbjct: 372 RGFNSEKLSR-YSSVGLLAPNMQAKVVDWSSGSFLPPGNRGELWIQGPGVMKGYLNNPKA 431

Query: 446 TMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQVAPTDLEAVVITHPEVLDT 505
           T  +I +++WL TGDI YFD DGYL++VDR+KE+IKYKGFQ+AP DLEAV+++HP ++D 
Sbjct: 432 TQMSIVEDSWLRTGDIAYFDEDGYLFIVDRIKEIIKYKGFQIAPADLEAVLVSHPLIIDA 491

Query: 506 AVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQQVAPYKRVRKVIFTESIPKSAAG 565
           AV AA +EECGEIPVAFVV++  + LS++DV+ YVA QVAPY++VRKV+   SIPKS  G
Sbjct: 492 AVTAAPNEECGEIPVAFVVRRQETTLSEEDVISYVASQVAPYRKVRKVVMVNSIPKSPTG 551

Query: 566 KVLRRELQKHLPAS 578
           K+LR+EL++ L  S
Sbjct: 552 KILRKELKRILTNS 561

[Close]

BLAST of PI0001531 vs. TAIR 10
Match: AT1G20510.1 (OPC-8:0 CoA ligase1 )

HSP 1 Score: 438.0 bits (1125), Expect = 1.2e-122
Identity = 243/550 (44.18%), Postives = 351/550 (63.82%), Query Frame = 0


Query: 27  RSERTNKYPNWYSPDTGICHSVHGYRKLPSDPFLDVVSFIFSFQFQHNGHSALIDSSTGN 86
           RS   N    +YS  T I         LP +P LDV +FI S    H G  A ID+STG 
Sbjct: 7   RSGFCNSNSTFYSKRTPI--------PLPPNPSLDVTTFISS--QAHRGRIAFIDASTGQ 66

Query: 87  SISYKELFPMVKSMASGLHNLGISQGDVVLLMLPNSIFYPIIILGALYLGAVITTMFPQS 146
           ++++ EL+  V+S+A  L  +GI +G VVLL+ PNSI +P++ L  + LGA+ITT  P +
Sbjct: 67  NLTFTELWRAVESVADCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLN 126

Query: 147 SSSEIKKRITDCNVRLAFTITQKIKNFEALGIK--TIGVPENTDFDLMKPMGFSSFYELI 206
           +S+EI K+I D N  LAFT +Q +    A   K   + + E     +          +  
Sbjct: 127 TSNEIAKQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGDVRRLVEMMKKE 186

Query: 207 SGGSDLIKKPVIRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFV-RFEASQYEYLP 266
             G+ +  K  + Q+DTA +L+SSGTTG+SKGV+ SHRN I+ ++  V RF +   E   
Sbjct: 187 PSGNRV--KERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDDGE--- 246

Query: 267 TKNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPI 326
               ++  VPMFHIYGL+ F  GL++ GS+++V+SKF++ +++ AI +++ T  P+VPPI
Sbjct: 247 --QRFICTVPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPI 306

Query: 327 LTALTRTAEKIGVQ-RFRSLKQVSCGAASSSKKTIDDFVHALPHVDFIQGYGMTESTAIG 386
           L A+   A++I  +    S+  V CG A  SK+  + F    P V  +QGYG+TEST IG
Sbjct: 307 LVAMVNGADQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTGIG 366

Query: 387 TRGFNTKNNARNYLSVGLLAPNTEARVVDLVAGSCMPPGETGELLLRGPGSMKGYLNNPE 446
               +T   +R Y + G L+ + E R+VD V G  + P +TGEL L+GP  MKGY +N E
Sbjct: 367 A-STDTVEESRRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNEE 426

Query: 447 ATMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQVAPTDLEAVVITHPEVLD 506
           AT ST+D E WL TGD+ Y D DG+++VVDRLKE+IKYKG+QVAP +LEA+++THPE+ D
Sbjct: 427 ATSSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITD 486

Query: 507 TAVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQQVAPYKRVRKVIFTESIPKSAA 566
            AV    D+E G+ P+A+VV+K GS+LS+K ++++VA+QVAPYKR+RKV F  SIPK+ +
Sbjct: 487 AAVIPFPDKEVGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIRKVAFVSSIPKNPS 538

Query: 567 GKVLRRELQK 573
           GK+LR++L K
Sbjct: 547 GKILRKDLIK 538

[Close]

BLAST of PI0001531 vs. TAIR 10
Match: AT4G05160.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 415.6 bits (1067), Expect = 6.5e-116
Identity = 228/538 (42.38%), Postives = 341/538 (63.38%), Query Frame = 0


Query: 43  GICHSVHGYRKLPSDPFLDVVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMVKSMAS 102
           GI  S+     LP DP   +VSF+F     +    A+ DS TG+S+++ +L   V  +A 
Sbjct: 10  GIYRSLRPTLVLPKDPNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQLKSAVARLAH 69

Query: 103 GLHNLGISQGDVVLLMLPNSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITDCNVRL 162
           G H LGI + DVVL+  PNS  +P+  L    +G V TT  P  + +E+ K+I D N ++
Sbjct: 70  GFHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSKQIKDSNPKI 129

Query: 163 AFTITQ---KIKNFE----ALGIK-TIGVPENTDFDLMKPMGFSSFYELISGGSDLIKKP 222
             ++ Q   KIK F+     LG K T+ +P  ++    K + F +  EL    S+     
Sbjct: 130 IISVNQLFDKIKGFDLPVVLLGSKDTVEIPPGSN---SKILSFDNVMELSEPVSE-YPFV 189

Query: 223 VIRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFVRFEASQYEYLPTKNVYLAAVPM 282
            I+Q DTAA+L+SSGTTG SKGV L+H NFI+   +    +    EY     V+L  +PM
Sbjct: 190 EIKQSDTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGEY---HGVFLCFLPM 249

Query: 283 FHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPILTALTRTAEKI 342
           FH++GL++     +  G+++V M++F+++ V+K I++F+VTH  VVPP+  AL++ +   
Sbjct: 250 FHVFGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSKQSI-- 309

Query: 343 GVQRF--RSLKQVSCGAASSSKKTIDDFVHALPHVDFIQGYGMTESTAIGTRGFNTKNNA 402
            V++F   SLK +  GAA   K  +++    +P+V  +QGYGMTE+  I     + +   
Sbjct: 310 -VKKFDLSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTETCGI-VSVEDPRLGK 369

Query: 403 RNYLSVGLLAPNTEARVVDLVAGSCMPPGETGELLLRGPGSMKGYLNNPEATMSTIDQEN 462
           RN  S G+LAP  EA++V +  G   PP + GE+ +RGP  MKGYLNNP+AT  TID+++
Sbjct: 370 RNSGSAGMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKKS 429

Query: 463 WLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQVAPTDLEAVVITHPEVLDTAVAAAKDEE 522
           W+HTGD+ YF+ DG LYVVDR+KE+IKYKGFQVAP +LE ++++HP++LD  V    DEE
Sbjct: 430 WVHTGDLGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDEE 489

Query: 523 CGEIPVAFVVKKPGSALSQKDVVDYVAQQVAPYKRVRKVIFTESIPKSAAGKVLRREL 571
            GE+P+AFVV+ P S+++++D+  ++A+QVAPYKR+R+V F   +PKSAAGK+LRREL
Sbjct: 490 AGEVPIAFVVRSPNSSITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKILRREL 536

[Close]

BLAST of PI0001531 vs. TAIR 10
Match: AT1G20480.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 404.4 bits (1038), Expect = 1.5e-112
Identity = 233/553 (42.13%), Postives = 338/553 (61.12%), Query Frame = 0


Query: 38  YSPDTGICHSVHGYRKLPSDPFLDVVSFIFSFQFQHNGHSALIDSSTGNSISYKELFPMV 97
           +   T I +S      LP + FLDV SFI S    H G +  +D+ TG  +S+ EL+  V
Sbjct: 27  FCESTSIFYSKREPMALPPNQFLDVTSFIAS--QPHRGKTVFVDAVTGRRLSFPELWLGV 86

Query: 98  KSMASGLHNLGISQGDVVLLMLPNSIFYPIIILGALYLGAVITTMFPQSSSSEIKKRITD 157
           + +A  L+ LG+ +G+VV+++ PNSI +PI+ L  + LGA+ITT  P ++S EI K+I D
Sbjct: 87  ERVAGCLYALGVRKGNVVIILSPNSILFPIVSLSVMSLGAIITTANPINTSDEISKQIGD 146

Query: 158 CNVRLAFTITQKIK--------NFEALGIKTIGVPENTDFDLMKPMG-FSSFYELISGGS 217
               LAFT  + +         N   + +    VP  +  D +K +G   +  E  +  S
Sbjct: 147 SRPVLAFTTCKLVSKLAAASNFNLPVVLMDDYHVPSQSYGDRVKLVGRLETMIE--TEPS 206

Query: 218 DLIKKPVIRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELF-VRFEASQYEYLPTKNV 277
           +   K  + Q+DTAA+L+SSGTTG SKGVMLSHRN I+ ++ +  RF   Q         
Sbjct: 207 ESRVKQRVNQDDTAALLYSSGTTGTSKGVMLSHRNLIALVQAYRARFGLEQRT------- 266

Query: 278 YLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPILTAL 337
            +  +PM HI+G   F  GL++LG ++VV+ KFD+  ++ A++  + ++  +VPPI+ A+
Sbjct: 267 -ICTIPMCHIFGFGGFATGLIALGWTIVVLPKFDMAKLLSAVETHRSSYLSLVPPIVVAM 326

Query: 338 TRTAEKIGVQ-RFRSLKQVSCGAASSSKKTIDDFVHALPHVDFIQGYGMTESTAIGTRGF 397
              A +I  +    SL  V  G A  S++  + FV   P V  +QGYG+TESTAI    F
Sbjct: 327 VNGANEINSKYDLSSLHTVVAGGAPLSREVTEKFVENYPKVKILQGYGLTESTAIAASMF 386

Query: 398 NTKNNARNYLSVGLLAPNTEARVVDLVAGSCMPPGETGELLLRGPGSMKGYLNNPEATMS 457
           N K   + Y + GLLAPN E ++VD   G  +   +TGEL +R P  MKGY  N EAT S
Sbjct: 387 N-KEETKRYGASGLLAPNVEGKIVDPDTGRVLGVNQTGELWIRSPTVMKGYFKNKEATAS 446

Query: 458 TIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQVAPTDLEAVVITHPEVLDTAVA 517
           TID E WL TGD+ Y D DG+++VVDRLKE+IK  G+QVAP +LEA+++ HPE+ D AV 
Sbjct: 447 TIDSEGWLKTGDLCYIDGDGFVFVVDRLKELIKCNGYQVAPAELEALLLAHPEIADAAVI 506

Query: 518 AAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQQVAPYKRVRKVIFTESIPKSAAGKVL 577
              D + G+ P+A++V+K GS LS+ +++ +VA+QV+PYK++RKV F  SIPK+ +GK+L
Sbjct: 507 PIPDMKAGQYPMAYIVRKVGSNLSESEIMGFVAKQVSPYKKIRKVTFLASIPKNPSGKIL 565

Query: 578 RRELQKHLPASKL 580
           RREL K L  SKL
Sbjct: 567 RRELTK-LTTSKL 565

[Close]

BLAST of PI0001531 vs. TAIR 10
Match: AT1G20500.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 400.2 bits (1027), Expect = 2.8e-111
Identity = 232/556 (41.73%), Postives = 341/556 (61.33%), Query Frame = 0


Query: 40  PDTGICHSVHGY------RKLPSDPFLDVVSFIFSFQFQHNGHSALIDSSTGNSISYKEL 99
           P +G C S   +        LP +   DV +FI S    H G +A ID++TG  +++ +L
Sbjct: 12  PRSGFCKSNSTFYSKRQPLSLPPNLSRDVTTFISS--QPHRGKTAFIDAATGQCLTFSDL 71

Query: 100 FPMVKSMASGL-HNLGISQGDVVLLMLPNSIFYPIIILGALYLGAVITTMFPQSSSSEIK 159
           +  V  +A  L H +GI +GDVVL++ PNSIF P++ L  + LGAV TT    ++S EI 
Sbjct: 72  WRAVDRVADCLYHEVGIRRGDVVLILSPNSIFIPVVCLSVMSLGAVFTTANTLNTSGEIS 131

Query: 160 KRITDCNVRLAFTITQKIKNFEALGIKTIGVPENTDFDLMKPMGFSSFYELISGGSDLIK 219
           K+I D N  L FT T+++     + I  +     TD ++ + +  +S   ++   S+++K
Sbjct: 132 KQIADSNPTLVFT-TRQLAPKLPVAISVV----LTDDEVYQEL--TSAIRVVGILSEMVK 191

Query: 220 KPV--------IRQEDTAAILFSSGTTGVSKGVMLSHRNFISTIELFVRFEASQYEYLPT 279
           K          + Q+DTA +L+SSGTTG SKGV+ SHRN  + +  F+       + L  
Sbjct: 192 KEPSGQRVRDRVNQDDTAMMLYSSGTTGPSKGVISSHRNLTAHVARFIS------DNLKR 251

Query: 280 KNVYLAAVPMFHIYGLSIFVMGLMSLGSSVVVMSKFDVKDVVKAIDRFKVTHFPVVPPIL 339
            ++++  VPMFH YGL  F MG ++LGS+VV++ +F + D++ A+++ + T   + PP+L
Sbjct: 252 DDIFICTVPMFHTYGLLTFAMGTVALGSTVVILRRFQLHDMMDAVEKHRATALALAPPVL 311

Query: 340 TALTRTAEKIGVQ-RFRSLKQVSCGAASSSKKTIDDFVHALPHVDFIQGYGMTESTAIGT 399
            A+   A+ I  +    SLK V CG A  SK+  + F+   P VD +QGY +TES   G 
Sbjct: 312 VAMINDADLIKAKYDLSSLKTVRCGGAPLSKEVTEGFLEKYPTVDILQGYALTESNG-GG 371

Query: 400 RGFNTKNNARNYLSVGLLAPNTEARVVDLVAGSCMPPGETGELLLRGPGSMKGYLNNPEA 459
              N+   +R Y + G L  + EAR+VD   G  M   +TGEL L+GP   KGY  N EA
Sbjct: 372 AFTNSAEESRRYGTAGTLTSDVEARIVDPNTGRFMGINQTGELWLKGPSISKGYFKNQEA 431

Query: 460 TMSTIDQENWLHTGDIVYFDRDGYLYVVDRLKEVIKYKGFQVAPTDLEAVVITHPEVLDT 519
           T  TI+ E WL TGD+ Y D DG+L+VVDRLKE+IKYKG+QV P +LEA++ITHP++LD 
Sbjct: 432 TNETINLEGWLKTGDLCYIDEDGFLFVVDRLKELIKYKGYQVPPAELEALLITHPDILDA 491

Query: 520 AVAAAKDEECGEIPVAFVVKKPGSALSQKDVVDYVAQQVAPYKRVRKVIFTESIPKSAAG 579
           AV    D+E G+ P+A+VV+K  S LS+K V+D++++QVAPYK++RKV F  SIPK+A+G
Sbjct: 492 AVIPFPDKEAGQYPMAYVVRKHESNLSEKQVIDFISKQVAPYKKIRKVSFINSIPKTASG 550

The following BLAST results are available for this feature:

BLAST of PI0001531 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84P24	4.3e-181	57.76	4-coumarate--CoA ligase-like 6 OS=Arabidopsis thaliana OX=3702 GN=4CLL6 PE=1 SV=...	[more]
Q6YYZ2	3.8e-145	51.72	4-coumarate--CoA ligase-like 3 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL3...	[more]
Q336M7	2.9e-129	49.25	4-coumarate--CoA ligase-like 2 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL2...	[more]
M4IQR7	1.5e-122	43.64	Probable CoA ligase CCL5 OS=Humulus lupulus OX=3486 GN=CCL5 PE=2 SV=1	[more]
Q84P21	1.7e-121	44.18	4-coumarate--CoA ligase-like 5 OS=Arabidopsis thaliana OX=3702 GN=4CLL5 PE=1 SV=...	[more]

BLAST of PI0001531 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L8X1	2.2e-310	94.47	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G638510 PE=4 SV=1	[more]
A0A5D3DC80	1.5e-309	94.30	4-coumarate--CoA ligase-like 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A1S3CCH7	1.7e-289	92.59	4-coumarate--CoA ligase-like 6 OS=Cucumis melo OX=3656 GN=LOC103499264 PE=4 SV=1	[more]
A0A5A7VLT7	8.4e-289	94.09	4-coumarate--CoA ligase-like 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s...	[more]
A0A6J1HGK9	2.5e-269	81.94	4-coumarate--CoA ligase-like 6 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]

BLAST of PI0001531 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004144410.1	4.4e-310	94.47	4-coumarate--CoA ligase-like 6 [Cucumis sativus] >KGN58405.1 hypothetical protei...	[more]
TYK21080.1	3.1e-309	94.30	4-coumarate--CoA ligase-like 6 [Cucumis melo var. makuwa]	[more]
XP_008460452.1	3.5e-289	92.59	PREDICTED: 4-coumarate--CoA ligase-like 6 [Cucumis melo]	[more]
KAA0068160.1	1.7e-288	94.09	4-coumarate--CoA ligase-like 6 [Cucumis melo var. makuwa]	[more]
XP_038888724.1	5.0e-288	87.50	4-coumarate--CoA ligase-like 6 [Benincasa hispida]	[more]

BLAST of PI0001531 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G19010.1	3.0e-182	57.76	AMP-dependent synthetase and ligase family protein	[more]
AT1G20510.1	1.2e-122	44.18	OPC-8:0 CoA ligase1	[more]
AT4G05160.1	6.5e-116	42.38	AMP-dependent synthetase and ligase family protein	[more]
AT1G20480.1	1.5e-112	42.13	AMP-dependent synthetase and ligase family protein	[more]
AT1G20500.1	2.8e-111	41.73	AMP-dependent synthetase and ligase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR042099	ANL, N-terminal domain	GENE3D	3.40.50.12780		coord: 51..475 e-value: 6.7E-117 score: 392.8
IPR000873	AMP-dependent synthetase/ligase	PFAM	PF00501	AMP-binding	coord: 72..480 e-value: 1.2E-99 score: 333.8
None	No IPR available	GENE3D	3.30.300.30		coord: 476..576 e-value: 3.2E-29 score: 103.6
None	No IPR available	PANTHER	PTHR24096:SF261	4-COUMARATE--COA LIGASE-LIKE 6	coord: 29..573
None	No IPR available	PANTHER	PTHR24096	LONG-CHAIN-FATTY-ACID--COA LIGASE	coord: 29..573
None	No IPR available	CDD	cd05904	4CL	coord: 54..570 e-value: 0.0 score: 723.64
None	No IPR available	SUPERFAMILY	56801	Acetyl-CoA synthetase-like	coord: 39..574
IPR025110	AMP-binding enzyme, C-terminal domain	PFAM	PF13193	AMP-binding_C	coord: 490..564 e-value: 2.8E-16 score: 60.3
IPR020845	AMP-binding, conserved site	PROSITE	PS00455	AMP_BINDING	coord: 224..235

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0001531.1	PI0001531.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006744	ubiquinone biosynthetic process
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005777	peroxisome
molecular_function	GO:0016405	CoA-ligase activity