PI0001348 (gene) Melon (PI 482460) v1

Overview
NamePI0001348
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Description4-hydroxy-3-methylbut-2-enyl diphosphate reductase
Locationchr01: 40017 .. 44210 (-)
RNA-Seq ExpressionPI0001348
SyntenyPI0001348
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTTGAAAGAGTTCTTACCAATTTAAATTGTACTATAAGGCCTTCTCTTTTATAACAAAAATGCAAAAGAAAAAGAAAAGACAACAGATAATATTGTAATGATAATTTCTAATTCTAATGTAAATTTATCAACTTAAGAAAAGAAAAGAAAAAAAATAAAGAAAAAATAAAGAAGAAGACAAAGGGCATTGTTGAATTTGAGATAAATGGAGCAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGGGGCCATTTTTGTCTGAGCTTGCGACTGGAAATTGAGCTCTCAAATTACTCTCTTCCATGGCGTTCTCTATGCAACTTTATCGTCTTCCCACTCGTACGGATGCAGCCTTGCCGGAGAATTTCACCGCCGTTCGATCACTCCGGTACAGGAAGCCTATGTCCGTGAGATGCGACGGCGATTCTTCTTCTTCTTCTTCTTCTTCTTCTGCGGCTGTGGATTCCAATTTTGACGCCAAGGTGTTCCGGAGGAACTTGACGAAGAGTGACAATTACAACCGCAAAGGCTTTGGTTACAAGGACGAGACTCTGGCGCTCATGAATCGAGTATACGAGTAAGCCTCGATCGTCTTGAATTTGTTAGATTTCTAATTGAATTAGGAAATTATTTATGAATTCTTTGTTTTCATTCTTTTGCATGTCAGAGAATATTCTATTACCTGCTCCTGAAGAATTTTAGTCTTTCGATTTGGCTTCTCAAGGCCTTAAACTAAATTAAAAATAATAAAAAAAAAAGAAGGGGGTAAAGGCTTATCACTTGAATTTTCAACTTATTTTTATGAAAATAAGTTTTTTAAGGAAGCTTGTGTTTTCTAATTCTATGTTCGAATTTCAGGTGATATCATAAAGACTCTGAAGGAAAATGGATTTGAGTATACGTGGGGAGATGTTACTGTAAAACTGGCGGAAGCATATGGATTTTGTTGGGGAGTCGAGCGAGCAGTTCAGATTGCTTATGAAGCGAGAAAACAATTTCCTGAGGAGAAAATCTGGTTAACTAATGAGATTATTCACAATCCAACTGTTAACAAGGTCTTGATTTATATTTAGTGCTTGATTCTTTAGATTTTTAAATCTAGAAATGAATCTTACTCTGGTAAACTTGATGATGCTTATTGTGTTCTATTTGGTAGAGGGGAAAAAAAGTTAAAGCTTTGTGTAATATTTTATTGTCCAATGACGAGTTGTTAGGATCTGTTCTACTTTCAATCAGAGGTGTTTCTTTTATCCCTTTCTGGGATCTTTCCCTTTCTTTCTGACTGAACCTGTTAGGTATCAAGTTTTCAGACAGAAAAAAACCCCCAAGGACACTTACTGAGGCAATAAACTCTGTTTATGATACTCAGAGAAAGGAAACAGCCAACAATTATAAGATAGTTCAAATACCAAAAAGAAACAAGAACTCAGTGACAAGTAGAAGAACAAGGAAGAAATAAAATATAAGAAATGACAGTATTCGAGAGCTGCCTCTTCTCCCAAAAGCTTCAAAAGCTTTCCAATTCATCACCCCAAAATGATTCCCCAAATCCCTTCATAAACCCCTTACCGATGGTTCCCACCTCATATCACATTCTGTGCCAACATTCTCTTTAATTCCCAACTTTCCTCTTTTACCCCTCCTCTGGTATGTGAATAATGGGAGGTCTAACAGACTCGTGCATCTTATTTGTAGAGGAAGTTCAGATTTTCAATTACTGGTTATTACTGACTAGTGTGTTGACGTCTCTCTTTTATTCATCTAATGATGACATGCTCCAGCCGGAAAAAGGCGATCTGTTTTTTTTCTTGAATTTTCATTTCCATCCCAACTATGCATTTTTATCAGTCTGAACTGTGATTTGAAAATTTGCAAGATGGTATCCCAGTTGCGGTCCTGTCCCATGAGTGTGGTTTTCAGTCTGGTGAATGACTATGAGGAATGAAGAGCTTTGACCTTCCAGTCTTTGGGAACAATGCAATTCTCACCCTAATTTTGTGGAATTCTAAATTTATCATTTTGATCTTTTTATTTTCTAAATTCTAATCATGTTTCCATCTAGTGTATGGAGTAGGATAGACTTGTTGACCAACTTATAGTGTTGGAAGCAGTAAAAAGTCTTGCACCAATACTTGGATTTTTGTAAGATAAAAATTTATCTTTACTTCTGTCCTTTTCCTAGAAACTTTGAATATCCATTTTTGGATTTCCTTGCTATCTTTTCCAACAAAAGAACATCTCGAGTATGACTGGTGTTCAAAGTTTCTAAACTTAATTGTTCGTACCATCCTTTCGCATAATTCTTAGTTTTCTTGTTACCGAATGTAGAAATTTAATACATATTTTCGAATTTTATATGTAGCGTTTGGAGGAGATGGAAGTCCAAAACATTCCACTTGATGAAGGAAGGAAACAGTTTGAAGTGGTTGATAAGGGTGATGTGTGATTTTTGCCTGCATTTGGAGCAGCAGTGGAGGAGATGTTGACATTGAGCGAAAAAAAGTGCAATCGTTGATACAACTTGCCCATGGGTTTCAAAGGTATCGTAGCAGTTTTTTTTTCTCTCCTTGCTGTACGTTCTGATACTGACAATAACGCTGCACTGCAATTAATACTATGTTTGTTGCATCTGACAAAATTACTCTCGATCCATACATTGGTTGCACAACATTTGAAGGTTTGGAATGTTGTGACAAGCACAAAAAAAGGATATTATACTTCAATCATTCACGGTAAATATGCTCATGAAGAAACTGTAGCGACAGCATCATTTGCAGGGAAATATATAATTGTAAAGAACATGGCAGAGTATGTCCATTTCGTTTTTCTTTTTTATTGACCACAGCAATTCTGCAATTGCTGGGTGAGGAATAATGTGAATTGTTGATAAAAACTTTTGCCTTTATTTATTCAGGCAATGTATGTGTGGACTATATTCTTGGAGGTGAACTTGATGGATCCAGTTCAACCAAAGAAGAGTTTATGAAGGTATTATTTACAATGCTTTCTTAACATTATATATTGGGATCGTTCACATTTCAGAGCATTATTGGTAACCATCCTGGTCTTCAGAGATTCAAAATGCAGTCTCGGAAGGTTTGACCCAGATCTTGACCTATTAAAGGTTGGGATTGCAAACCAAACCACAATGCTCAAGGGAGAAACAGAAGAGATTGGTAAATTATCTGCTGAACACTATTCATTGCTGTTCCTTGAAATTTCAAGCATATTTATTTAAGTTTTACTTTTATCAGGTAAATTAGTTGAAAGAACTATGATGCGCAAGCACGGAGTGGAAAACGTCAATGAGCACTTCCTCAGTTTCAATACAATATGTGATGTGCTACAAGTAAGTCTAGTCGGATTTGCTGCCGTGTCTAGGATGCGGATTTTAGGGAGTAAAAGGTGTTGTGTATTCTATGTTTTACTGCTTCAGTGCATGCATGTGTGCTCACTTGTGGTATTATCAATTAAACTCTTATCCTTTGGAACCATCTTTATACGCTCCTGTCATGATTCTACTGACAAGTTTGCTAACCCATGTATGCTTATCTCGTATACTTTTAGTTGTCACTTATGTGTGTTCTCTAACCTGTCTTATTCTATCAGGAGCGTCAAGATGCAATGTACAAGCTAGTAGAGGGAAAACTGGATCTTATGTTAGTTGTTGGTGGATGGAATTCCAGTAACACTTCTCATCTTCAAGAGATAGCGGAGGAGGAGCGAGGAATTCGTCTTACTGGATTGATAGTGAGCAGAGAATAGGTCCAGGAAACAGAATATCTTACAAACTGAATGTAAGTTACTATACAGATTTCAGTTCTAATGCCTTTAGTCTCAATTCCAAACTCTTTGTTCACTTTTCAATTACAAAATAAACCCTACCTTTCTTTCCTTGATTACTTACAATTTCACCATCCTTGCAAATTCATGTTTAGCATGGGGAGTTAGTTGAAAAGGAGAACTGGCTACCCAAGGGTCATATCACGATCGGTGTCACATCCGGGGCTTCAACCCCTGATAAGGTTAGTCTCTCTCCGACTTACATGCTTGTTAGTACAAACTGTTGTCTGCAACAAATTTAAAGCCATACCATGTTCGTATGTGCTATCAAACAGGTCGTAGAGGATGCTCTTATCAAAGTCTTCGACATTAAGCGTGAGGAAGCTTTGCATTTGCATAAATTTATAGGTTAG

mRNA sequence

GTTTGAAAGAGTTCTTACCAATTTAAATTGTACTATAAGGCCTTCTCTTTTATAACAAAAATGCAAAAGAAAAAGAAAAGACAACAGATAATATTGTAATGATAATTTCTAATTCTAATGTAAATTTATCAACTTAAGAAAAGAAAAGAAAAAAAATAAAGAAAAAATAAAGAAGAAGACAAAGGGCATTGTTGAATTTGAGATAAATGGAGCAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGGGGCCATTTTTGTCTGAGCTTGCGACTGGAAATTGAGCTCTCAAATTACTCTCTTCCATGGCGTTCTCTATGCAACTTTATCGTCTTCCCACTCGTACGGATGCAGCCTTGCCGGAGAATTTCACCGCCGTTCGATCACTCCGGTACAGGAAGCCTATGTCCGTGAGATGCGACGGCGATTCTTCTTCTTCTTCTTCTTCTTCTTCTGCGGCTGTGGATTCCAATTTTGACGCCAAGGTGTTCCGGAGGAACTTGACGAAGAGTGACAATTACAACCGCAAAGGCTTTGGTTACAAGGACGAGACTCTGGCGCTCATGAATCGAACTCTGAAGGAAAATGGATTTGAGTATACGTGGGGAGATGTTACTGTAAAACTGGCGGAAGCATATGGATTTTGTTGGGGAGTCGAGCGAGCAGTTCAGATTGCTTATGAAGCGAGAAAACAATTTCCTGAGGAGAAAATCTGGTTAACTAATGAGATTATTCACAATCCAACTGTTAACAAGCGTTTGGAGGAGATGGAAGTCCAAAACATTCCACTTGATGAAGGAAGGAAACAGTTTGAAGTGGTTGATAAGGGTGATGTTGCAATCGTTGATACAACTTGCCCATGGGTTTCAAAGGTTTGGAATGTTGTGACAAGCACAAAAAAAGGATATTATACTTCAATCATTCACGGTAAATATGCTCATGAAGAAACTGTAGCGACAGCATCATTTGCAGGGAAATATATAATTGTAAAGAACATGGCAGAAGATTCAAAATGCAGTCTCGGAAGGTTTGACCCAGATCTTGACCTATTAAAGGTTGGGATTGCAAACCAAACCACAATGCTCAAGGGAGAAACAGAAGAGATTGGTAAATTAGTTGAAAGAACTATGATGCGCAAGCACGGAGTGGAAAACGTCAATGAGCACTTCCTCAGTTTCAATACAATATGTGATGTGCTACAAGAGCGTCAAGATGCAATGTACAAGCTAGTAGAGGGAAAACTGGATCTTATGTTAGTTGTTGGTGGATGGAATTCCAGTAACACTTCTCATCTTCAAGAGATAGCGGAGGAGGATGAGCAGAGAATAGGTCCAGGAAACAGAATATCTTACAAACTGAATCATGGGGAGTTAGTTGAAAAGGAGAACTGGCTACCCAAGGGTCATATCACGATCGGTGTCACATCCGGGGCTTCAACCCCTGATAAGGTCGTAGAGGATGCTCTTATCAAAGTCTTCGACATTAAGCGTGAGGAAGCTTTGCATTTGCATAAATTTATAGGTTAG

Coding sequence (CDS)

ATGGCGTTCTCTATGCAACTTTATCGTCTTCCCACTCGTACGGATGCAGCCTTGCCGGAGAATTTCACCGCCGTTCGATCACTCCGGTACAGGAAGCCTATGTCCGTGAGATGCGACGGCGATTCTTCTTCTTCTTCTTCTTCTTCTTCTGCGGCTGTGGATTCCAATTTTGACGCCAAGGTGTTCCGGAGGAACTTGACGAAGAGTGACAATTACAACCGCAAAGGCTTTGGTTACAAGGACGAGACTCTGGCGCTCATGAATCGAACTCTGAAGGAAAATGGATTTGAGTATACGTGGGGAGATGTTACTGTAAAACTGGCGGAAGCATATGGATTTTGTTGGGGAGTCGAGCGAGCAGTTCAGATTGCTTATGAAGCGAGAAAACAATTTCCTGAGGAGAAAATCTGGTTAACTAATGAGATTATTCACAATCCAACTGTTAACAAGCGTTTGGAGGAGATGGAAGTCCAAAACATTCCACTTGATGAAGGAAGGAAACAGTTTGAAGTGGTTGATAAGGGTGATGTTGCAATCGTTGATACAACTTGCCCATGGGTTTCAAAGGTTTGGAATGTTGTGACAAGCACAAAAAAAGGATATTATACTTCAATCATTCACGGTAAATATGCTCATGAAGAAACTGTAGCGACAGCATCATTTGCAGGGAAATATATAATTGTAAAGAACATGGCAGAAGATTCAAAATGCAGTCTCGGAAGGTTTGACCCAGATCTTGACCTATTAAAGGTTGGGATTGCAAACCAAACCACAATGCTCAAGGGAGAAACAGAAGAGATTGGTAAATTAGTTGAAAGAACTATGATGCGCAAGCACGGAGTGGAAAACGTCAATGAGCACTTCCTCAGTTTCAATACAATATGTGATGTGCTACAAGAGCGTCAAGATGCAATGTACAAGCTAGTAGAGGGAAAACTGGATCTTATGTTAGTTGTTGGTGGATGGAATTCCAGTAACACTTCTCATCTTCAAGAGATAGCGGAGGAGGATGAGCAGAGAATAGGTCCAGGAAACAGAATATCTTACAAACTGAATCATGGGGAGTTAGTTGAAAAGGAGAACTGGCTACCCAAGGGTCATATCACGATCGGTGTCACATCCGGGGCTTCAACCCCTGATAAGGTCGTAGAGGATGCTCTTATCAAAGTCTTCGACATTAAGCGTGAGGAAGCTTTGCATTTGCATAAATTTATAGGTTAG

Protein sequence

MAFSMQLYRLPTRTDAALPENFTAVRSLRYRKPMSVRCDGDSSSSSSSSSAAVDSNFDAKVFRRNLTKSDNYNRKGFGYKDETLALMNRTLKENGFEYTWGDVTVKLAEAYGFCWGVERAVQIAYEARKQFPEEKIWLTNEIIHNPTVNKRLEEMEVQNIPLDEGRKQFEVVDKGDVAIVDTTCPWVSKVWNVVTSTKKGYYTSIIHGKYAHEETVATASFAGKYIIVKNMAEDSKCSLGRFDPDLDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDVLQERQDAMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEEDEQRIGPGNRISYKLNHGELVEKENWLPKGHITIGVTSGASTPDKVVEDALIKVFDIKREEALHLHKFIG
Homology
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BLAST of PI0001348 vs. ExPASy Swiss-Prot
Match: Q94B35 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISPH PE=1 SV=1)

HSP 1 Score: 559.3 bits (1440), Expect = 3.6e-158
Identity = 296/466 (63.52%), Postives = 349/466 (74.89%), Query Frame = 0 

Query: 1   MAFSMQLYRLPTRTDAALPEN-FTAVRSLRYRKPMSVRCDGDSSSSSSSSSAAVDSNFDA 60
           MA ++Q  RL  R D  + EN  +   SLR RK +SVRC      ++ S S  +DS+FDA
Sbjct: 1   MAVALQFSRLCVRPDTFVRENHLSGSGSLRRRKALSVRC-SSGDENAPSPSVVMDSDFDA 60

Query: 61  KVFRRNLTKSDNYNRKGFGYKDETLALMNR--------TLKENGFEYTWGDVTVKLAEAY 120
           KVFR+NLT+SDNYNRKGFG+K+ETL LMNR        TLK NG+ Y+WGDVTVKLA+AY
Sbjct: 61  KVFRKNLTRSDNYNRKGFGHKEETLKLMNREYTSDILETLKTNGYTYSWGDVTVKLAKAY 120

Query: 121 GFCWGVERAVQIAYEARKQFPEEKIWLTNEIIHNPTVNKRLEEMEVQNIPLDEGRKQFEV 180
           GFCWGVERAVQIAYEARKQFPEE++W+TNEIIHNPTVNKRLE+M+V+ IP+++ +KQF+V
Sbjct: 121 GFCWGVERAVQIAYEARKQFPEERLWITNEIIHNPTVNKRLEDMDVKIIPVEDSKKQFDV 180

Query: 181 VDKGDVA---------------------IVDTTCPWVSKVWNVVTSTKKGYYTSIIHGKY 240
           V+K DV                      IVDTTCPWV+KVWN V   KKG YTS+IHGKY
Sbjct: 181 VEKDDVVILPAFGAGVDEMYVLNDKKVQIVDTTCPWVTKVWNTVEKHKKGEYTSVIHGKY 240

Query: 241 AHEETVATASFAGKYIIVKNMAEDS-------------------------KCSLGR-FDP 300
            HEET+ATASFAGKYIIVKNM E +                         K ++ + FDP
Sbjct: 241 NHEETIATASFAGKYIIVKNMKEANYVCDYILGGQYDGSSSTKEEFMEKFKYAISKGFDP 300

Query: 301 DLDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDVLQERQDA 360
           D DL+KVGIANQTTMLKGETEEIG+L+E TMMRK+GVENV+ HF+SFNTICD  QERQDA
Sbjct: 301 DNDLVKVGIANQTTMLKGETEEIGRLLETTMMRKYGVENVSGHFISFNTICDATQERQDA 360

Query: 361 MYKLVEGKLDLMLVVGGWNSSNTSHLQEIAE---------EDEQRIGPGNRISYKLNHGE 402
           +Y+LVE K+DLMLVVGGWNSSNTSHLQEI+E         + E+RIGPGN+I+YKL++GE
Sbjct: 361 IYELVEEKIDLMLVVGGWNSSNTSHLQEISEARGIPSYWIDSEKRIGPGNKIAYKLHYGE 420

[Close]
BLAST of PI0001348 vs. ExPASy Swiss-Prot
Match: Q6AVG6 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISPH PE=2 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 2.6e-156
Identity = 294/433 (67.90%), Postives = 334/433 (77.14%), Query Frame = 0 

Query: 31  RKPMSVRCDGDSSSSSSSSSAAVDSNFDAKVFRRNLTKSDNYNRKGFGYKDETLALMN-- 90
           R P SVRCD  S++SS S+SA++D++FD K FR NLT+SDNYNRKGFG+K ETL LM+  
Sbjct: 25  RAPSSVRCD-SSAASSLSASASLDADFDKKQFRHNLTRSDNYNRKGFGHKKETLELMSQE 84

Query: 91  ------RTLKENGFEYTWGDVTVKLAEAYGFCWGVERAVQIAYEARKQFPEEKIWLTNEI 150
                 +TLKENG ++TWG VTVKLAEAYGFCWGVERAVQIAYEARKQFP+++IWLTNEI
Sbjct: 85  YTSDVIKTLKENGNQHTWGPVTVKLAEAYGFCWGVERAVQIAYEARKQFPDDRIWLTNEI 144

Query: 151 IHNPTVNKRLEEMEVQNIPLDEGRKQFEVVDKGDVA---------------------IVD 210
           IHNPTVNKRLE+M VQNIP+D G K F+VV++GDV                      IVD
Sbjct: 145 IHNPTVNKRLEDMGVQNIPVDAGIKDFDVVEQGDVVVLPAFGAAVEEMYTLNEKKVQIVD 204

Query: 211 TTCPWVSKVWNVVTSTKKGYYTSIIHGKYAHEETVATASFAGKYIIVKNMAEDS-KCS-- 270
           TTCPWVSKVWN+V   KKG YTSIIHGKY+HEETVATASFAG YIIVKN+AE S  C   
Sbjct: 205 TTCPWVSKVWNMVEKHKKGDYTSIIHGKYSHEETVATASFAGTYIIVKNIAEASYVCDYI 264

Query: 271 LG-----------------------RFDPDLDLLKVGIANQTTMLKGETEEIGKLVERTM 330
           LG                        FDPD+DL+KVGIANQTTMLKGETEEIGKLVE+TM
Sbjct: 265 LGGQLDGSSSTKEEFLEKFKNAVSPGFDPDVDLVKVGIANQTTMLKGETEEIGKLVEKTM 324

Query: 331 MRKHGVENVNEHFLSFNTICDVLQERQDAMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAE 390
           MR+ GVENVN+HF++FNTICD  QERQDAMY+LV+ K+DL+LVVGGWNSSNTSHLQEI E
Sbjct: 325 MRRFGVENVNDHFIAFNTICDATQERQDAMYQLVKEKVDLILVVGGWNSSNTSHLQEIGE 384

Query: 391 ---------EDEQRIGPGNRISYKLNHGELVEKENWLPKGHITIGVTSGASTPDKVVEDA 400
                    + EQRIGPGN+ISYKLNHGELVEKENWLP+G ITIGVTSGASTPDKVVEDA
Sbjct: 385 LSGIPSYWIDSEQRIGPGNKISYKLNHGELVEKENWLPEGPITIGVTSGASTPDKVVEDA 444

[Close]
BLAST of PI0001348 vs. ExPASy Swiss-Prot
Match: Q31S64 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Synechococcus elongatus (strain PCC 7942 / FACHB-805) OX=1140 GN=ispH PE=3 SV=1)

HSP 1 Score: 410.6 bits (1054), Expect = 2.1e-113
Identity = 210/397 (52.90%), Postives = 268/397 (67.51%), Query Frame = 0 

Query: 58  DAKVFRRNLTKSDNYNRKGFGYKDETLALMN--------RTLKENGFEYTWGDVTVKLAE 117
           D K F+R L +SD YNRKGFG   +    +         ++L++NG+    G++T++LAE
Sbjct: 2   DTKAFKRALHQSDRYNRKGFGKTTDVSGALESAYQSDLIQSLRQNGYRLQRGEITIRLAE 61

Query: 118 AYGFCWGVERAVQIAYEARKQFPEEKIWLTNEIIHNPTVNKRLEEMEVQNIPLDEGRKQF 177
           A+GFCWGVERAV IAYE R+ FP+E+IW+TNEIIHNP+VN+ L EM V+ IP + G K F
Sbjct: 62  AFGFCWGVERAVAIAYETRQHFPQERIWITNEIIHNPSVNQHLREMSVEFIPCERGEKDF 121

Query: 178 EVVDKGDVA---------------------IVDTTCPWVSKVWNVVTSTKKGYYTSIIHG 237
            VVD+GDV                      IVDTTCPWVSKVWN V   K+G +TSIIHG
Sbjct: 122 SVVDRGDVVILPAFGASVQEMQLLDEKGCHIVDTTCPWVSKVWNTVEKHKRGAHTSIIHG 181

Query: 238 KYAHEETVATASFAGKYIIVKNMAE---------------------DSKCSLGRFDPDLD 297
           KY HEETVAT+SFA  Y++V N+ +                        CS G FDPD D
Sbjct: 182 KYNHEETVATSSFAETYLVVLNLEQAQYVCDYILNGGDRDEFMTRFGKACSAG-FDPDRD 241

Query: 298 LLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDVLQERQDAMYK 357
           L ++GIANQTTMLK ETE IGKL ERT+++K+G + +N+HFL+FNTICD  QERQDAM++
Sbjct: 242 LERIGIANQTTMLKSETEAIGKLFERTLLKKYGPQALNDHFLAFNTICDATQERQDAMFQ 301

Query: 358 LVEGKLDLMLVVGGWNSSNTSHLQEIAEEDE---------QRIGPGNRISYKLNHGELVE 396
           LVE  LDL++V+GG+NSSNT+HLQEIA E +         +RIGPGNRI +K  H +L  
Sbjct: 302 LVEEPLDLIVVIGGFNSSNTTHLQEIAIERQIPSFHIDAAERIGPGNRIEHKPLHTDLTT 361

[Close]
BLAST of PI0001348 vs. ExPASy Swiss-Prot
Match: Q5N249 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) OX=269084 GN=ispH PE=3 SV=1)

HSP 1 Score: 410.6 bits (1054), Expect = 2.1e-113
Identity = 210/397 (52.90%), Postives = 268/397 (67.51%), Query Frame = 0 

Query: 58  DAKVFRRNLTKSDNYNRKGFGYKDETLALMN--------RTLKENGFEYTWGDVTVKLAE 117
           D K F+R L +SD YNRKGFG   +    +         ++L++NG+    G++T++LAE
Sbjct: 2   DTKAFKRALHQSDRYNRKGFGKTTDVSGALESAYQSDLIQSLRQNGYRLQRGEITIRLAE 61

Query: 118 AYGFCWGVERAVQIAYEARKQFPEEKIWLTNEIIHNPTVNKRLEEMEVQNIPLDEGRKQF 177
           A+GFCWGVERAV IAYE R+ FP+E+IW+TNEIIHNP+VN+ L EM V+ IP + G K F
Sbjct: 62  AFGFCWGVERAVAIAYETRQHFPQERIWITNEIIHNPSVNQHLREMSVEFIPCERGEKDF 121

Query: 178 EVVDKGDVA---------------------IVDTTCPWVSKVWNVVTSTKKGYYTSIIHG 237
            VVD+GDV                      IVDTTCPWVSKVWN V   K+G +TSIIHG
Sbjct: 122 SVVDRGDVVILPAFGASVQEMQLLDEKGCHIVDTTCPWVSKVWNTVEKHKRGAHTSIIHG 181

Query: 238 KYAHEETVATASFAGKYIIVKNMAE---------------------DSKCSLGRFDPDLD 297
           KY HEETVAT+SFA  Y++V N+ +                        CS G FDPD D
Sbjct: 182 KYNHEETVATSSFAETYLVVLNLEQAQYVCDYILNGGDRDEFMTRFGKACSAG-FDPDRD 241

Query: 298 LLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDVLQERQDAMYK 357
           L ++GIANQTTMLK ETE IGKL ERT+++K+G + +N+HFL+FNTICD  QERQDAM++
Sbjct: 242 LERIGIANQTTMLKSETEAIGKLFERTLLKKYGPQALNDHFLAFNTICDATQERQDAMFQ 301

Query: 358 LVEGKLDLMLVVGGWNSSNTSHLQEIAEEDE---------QRIGPGNRISYKLNHGELVE 396
           LVE  LDL++V+GG+NSSNT+HLQEIA E +         +RIGPGNRI +K  H +L  
Sbjct: 302 LVEEPLDLIVVIGGFNSSNTTHLQEIAIERQIPSFHIDAAERIGPGNRIEHKPLHTDLTT 361

[Close]
BLAST of PI0001348 vs. ExPASy Swiss-Prot
Match: B8HWD3 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Cyanothece sp. (strain PCC 7425 / ATCC 29141) OX=395961 GN=ispH PE=3 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 4.6e-113
Identity = 215/396 (54.29%), Postives = 271/396 (68.43%), Query Frame = 0 

Query: 58  DAKVFRRNLTKSDNYNRKGFGYKDETLALMN--------RTLKENGFEYTWGDVTVKLAE 117
           D K F+R+L  S+ Y+RKGFG++ E   ++         + ++ENG  +  G+VT++LAE
Sbjct: 2   DTKAFKRSLHSSNQYHRKGFGHEAEVTEMLRFEYQSSLIQQIRENGNRFQRGEVTIQLAE 61

Query: 118 AYGFCWGVERAVQIAYEARKQFPEEKIWLTNEIIHNPTVNKRLEEMEVQNIPLDE-GRKQ 177
           A+GFCWGVERAV +AYE R+ FP E+IW+TNEIIHNP+VN+ L EM+V+ IP+DE G+K 
Sbjct: 62  AFGFCWGVERAVALAYETRQHFPTERIWITNEIIHNPSVNQNLREMQVEFIPVDEQGQKD 121

Query: 178 FEVVDKGDVA---------------------IVDTTCPWVSKVWNVVTSTKKGYYTSIIH 237
           F VV   DV                      I+DTTCPWVSKVWN V   KKG YTSIIH
Sbjct: 122 FSVVAGEDVVILPAFGASVQEMQLLNDKGCKIMDTTCPWVSKVWNSVEKHKKGSYTSIIH 181

Query: 238 GKYAHEETVATASFAGKYIIVKNMAE------------DSKCSLGR--------FDPDLD 297
           GKY HEET+AT+S+A  Y+IV ++ E            D +  L +        FDPD D
Sbjct: 182 GKYQHEETIATSSYAATYLIVLDLKEAEYVCNYILNGGDREEFLHKFRHAYSPDFDPDRD 241

Query: 298 LLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDVLQERQDAMYK 357
           L++VGIANQTTMLKGETE+IGKL ERT+MRK+G + +N+HFLSFNTICD  QERQDAM+K
Sbjct: 242 LVRVGIANQTTMLKGETEQIGKLFERTLMRKYGPDQINQHFLSFNTICDATQERQDAMFK 301

Query: 358 LVEGKLDLMLVVGGWNSSNTSHLQEIAEEDE---------QRIGPGNRISYKLNHGELVE 395
           LVE  LDLM+V+GG+NSSNT+HLQEI+ E E          RIGPGNR+ +K  H  L  
Sbjct: 302 LVEYPLDLMVVIGGFNSSNTTHLQEISIEREIPSYHIDSADRIGPGNRVEHKPLHRPLEI 361

[Close]
BLAST of PI0001348 vs. ExPASy TrEMBL
Match: A0A0A0K3D8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G075680 PE=3 SV=1)

HSP 1 Score: 709.5 bits (1830), Expect = 7.9e-201
Identity = 377/465 (81.08%), Postives = 384/465 (82.58%), Query Frame = 0 

Query: 1   MAFSMQLYRLPTRTDAALPENFTAVRSLRYRKPMSVRCDGD--SSSSSSSSSAAVDSNFD 60
           MAFSMQLYRLPTRTDAA+PENFTAVRSLRYRKPMSVRCDGD  SSSSSSSSSAAVDSNFD
Sbjct: 1   MAFSMQLYRLPTRTDAAMPENFTAVRSLRYRKPMSVRCDGDSSSSSSSSSSSAAVDSNFD 60

Query: 61  AKVFRRNLTKSDNYNRKGFGYKDETLALMNR--------TLKENGFEYTWGDVTVKLAEA 120
           AKVFRRNLTKSDNYNRKGFGYKDETLALMNR        TLKENGFEYTWGDVTVKLAEA
Sbjct: 61  AKVFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEA 120

Query: 121 YGFCWGVERAVQIAYEARKQFPEEKIWLTNEIIHNPTVNKRLEEMEVQNIPLDEGRKQFE 180
           YGFCWGVERAVQIAYEARKQFP+EKIWLTNEIIHNPTVNKRLEEMEVQNIPL++GRKQFE
Sbjct: 121 YGFCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEDGRKQFE 180

Query: 181 VVDKGDVA---------------------IVDTTCPWVSKVWNVVTSTKKGYYTSIIHGK 240
           VVDKGDV                      IVDTTCPWVSKVWNVV   KKG YTSIIHGK
Sbjct: 181 VVDKGDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGK 240

Query: 241 YAHEETVATASFAGKYIIVKNMAE-------------DSKCSLGR-------------FD 300
           YAHEETVATASFAG +IIVKNMAE             D   S                FD
Sbjct: 241 YAHEETVATASFAGTFIIVKNMAEAIYVCDYILGGELDGSSSTKEEFMKKFKNAVSKGFD 300

Query: 301 PDLDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDVLQERQD 360
           PD+DLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICD  QERQD
Sbjct: 301 PDVDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQD 360

Query: 361 AMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEE---------DEQRIGPGNRISYKLNHG 400
           AMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEE          EQRIGPGNRI+YKLNHG
Sbjct: 361 AMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHG 420

[Close]
BLAST of PI0001348 vs. ExPASy TrEMBL
Match: A0A5A7SJM1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00040 PE=3 SV=1)

HSP 1 Score: 704.5 bits (1817), Expect = 2.5e-199
Identity = 375/463 (80.99%), Postives = 381/463 (82.29%), Query Frame = 0 

Query: 1   MAFSMQLYRLPTRTDAALPENFTAVRSLRYRKPMSVRCDGDSSSSSSSSSAAVDSNFDAK 60
           MAFSMQLYRLPTRTDAALPEN +AVRSLRYRKPMSVRCDGD   SSSSSSAAVDS+FDAK
Sbjct: 1   MAFSMQLYRLPTRTDAALPENSSAVRSLRYRKPMSVRCDGD---SSSSSSAAVDSDFDAK 60

Query: 61  VFRRNLTKSDNYNRKGFGYKDETLALMNR--------TLKENGFEYTWGDVTVKLAEAYG 120
           VFRRNLTKSDNYNRKGFGYKDETLALMNR        TLKENGFEYTWGDVTVKLAEAYG
Sbjct: 61  VFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEAYG 120

Query: 121 FCWGVERAVQIAYEARKQFPEEKIWLTNEIIHNPTVNKRLEEMEVQNIPLDEGRKQFEVV 180
           FCWGVERAVQIAYEARKQFP+EKIWLTNEIIHNPTVNKRLEEMEVQNIPL+EGRKQFEVV
Sbjct: 121 FCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEEGRKQFEVV 180

Query: 181 DKGDVA---------------------IVDTTCPWVSKVWNVVTSTKKGYYTSIIHGKYA 240
           DKGDV                      IVDTTCPWVSKVWNVV   KKG YTSIIHGKYA
Sbjct: 181 DKGDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGKYA 240

Query: 241 HEETVATASFAGKYIIVKNMAE-------------DSKCSLGR-------------FDPD 300
           HEETVATASFAGKYIIVKNMAE             D   S                FDPD
Sbjct: 241 HEETVATASFAGKYIIVKNMAEAMYVCDYILGGELDGSSSTKEEFMKKFKNAVSEGFDPD 300

Query: 301 LDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDVLQERQDAM 360
           +DLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICD  QERQDAM
Sbjct: 301 VDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQDAM 360

Query: 361 YKLVEGKLDLMLVVGGWNSSNTSHLQEIAEE---------DEQRIGPGNRISYKLNHGEL 400
           YKLVEGKLDLMLVVGGWNSSNTSHLQEIAEE          EQRIGPGNRI+YKLNHGEL
Sbjct: 361 YKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHGEL 420

[Close]
BLAST of PI0001348 vs. ExPASy TrEMBL
Match: A0A1S3BZP5 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103495189 PE=3 SV=1)

HSP 1 Score: 704.5 bits (1817), Expect = 2.5e-199
Identity = 375/463 (80.99%), Postives = 381/463 (82.29%), Query Frame = 0 

Query: 1   MAFSMQLYRLPTRTDAALPENFTAVRSLRYRKPMSVRCDGDSSSSSSSSSAAVDSNFDAK 60
           MAFSMQLYRLPTRTDAALPEN +AVRSLRYRKPMSVRCDGD   SSSSSSAAVDS+FDAK
Sbjct: 1   MAFSMQLYRLPTRTDAALPENSSAVRSLRYRKPMSVRCDGD---SSSSSSAAVDSDFDAK 60

Query: 61  VFRRNLTKSDNYNRKGFGYKDETLALMNR--------TLKENGFEYTWGDVTVKLAEAYG 120
           VFRRNLTKSDNYNRKGFGYKDETLALMNR        TLKENGFEYTWGDVTVKLAEAYG
Sbjct: 61  VFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEAYG 120

Query: 121 FCWGVERAVQIAYEARKQFPEEKIWLTNEIIHNPTVNKRLEEMEVQNIPLDEGRKQFEVV 180
           FCWGVERAVQIAYEARKQFP+EKIWLTNEIIHNPTVNKRLEEMEVQNIPL+EGRKQFEVV
Sbjct: 121 FCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEEGRKQFEVV 180

Query: 181 DKGDVA---------------------IVDTTCPWVSKVWNVVTSTKKGYYTSIIHGKYA 240
           DKGDV                      IVDTTCPWVSKVWNVV   KKG YTSIIHGKYA
Sbjct: 181 DKGDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGKYA 240

Query: 241 HEETVATASFAGKYIIVKNMAE-------------DSKCSLGR-------------FDPD 300
           HEETVATASFAGKYIIVKNMAE             D   S                FDPD
Sbjct: 241 HEETVATASFAGKYIIVKNMAEAMYVCDYILGGELDGSSSTKEEFMKKFKNAVSEGFDPD 300

Query: 301 LDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDVLQERQDAM 360
           +DLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICD  QERQDAM
Sbjct: 301 VDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQDAM 360

Query: 361 YKLVEGKLDLMLVVGGWNSSNTSHLQEIAEE---------DEQRIGPGNRISYKLNHGEL 400
           YKLVEGKLDLMLVVGGWNSSNTSHLQEIAEE          EQRIGPGNRI+YKLNHGEL
Sbjct: 361 YKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHGEL 420

[Close]
BLAST of PI0001348 vs. ExPASy TrEMBL
Match: A0A6J1I4K4 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111470927 PE=3 SV=1)

HSP 1 Score: 672.9 bits (1735), Expect = 8.2e-190
Identity = 356/465 (76.56%), Postives = 376/465 (80.86%), Query Frame = 0 

Query: 1   MAFSMQLYRLPTRTDAALPENFTAVRSLRYRKPMSVRCDGDS--SSSSSSSSAAVDSNFD 60
           MAFSMQLYRL TRTDAALPE+F  VRS+R RKP+SVRCDGDS  S+SSSSSS AVDSNFD
Sbjct: 1   MAFSMQLYRLATRTDAALPESFPGVRSVRCRKPLSVRCDGDSSASASSSSSSVAVDSNFD 60

Query: 61  AKVFRRNLTKSDNYNRKGFGYKDETLALMN--------RTLKENGFEYTWGDVTVKLAEA 120
           AKVFRRNL+KS+NYNR+GFG+KDETLALMN        +TLKENGFEYTWGDVTVKLAEA
Sbjct: 61  AKVFRRNLSKSENYNRRGFGHKDETLALMNSEYTSDIIKTLKENGFEYTWGDVTVKLAEA 120

Query: 121 YGFCWGVERAVQIAYEARKQFPEEKIWLTNEIIHNPTVNKRLEEMEVQNIPLDEGRKQFE 180
           YGFCWGVERAVQIAYEARKQFP+EK+WLTNEIIHNPTVNKRLEEMEVQNIPL++G+KQFE
Sbjct: 121 YGFCWGVERAVQIAYEARKQFPDEKLWLTNEIIHNPTVNKRLEEMEVQNIPLEDGKKQFE 180

Query: 181 VVDKGDVA---------------------IVDTTCPWVSKVWNVVTSTKKGYYTSIIHGK 240
           VV KGDV                      IVDTTCPWVSKVWNVV   KKG +TSIIHGK
Sbjct: 181 VVGKGDVVILPAFGAGVDEMLTLSEKKVQIVDTTCPWVSKVWNVVEKHKKGEFTSIIHGK 240

Query: 241 YAHEETVATASFAGKYIIVKNMAE-------------DSKCSLGR-------------FD 300
           Y+HEETVATASFAGKYIIVKNMAE             D   S                FD
Sbjct: 241 YSHEETVATASFAGKYIIVKNMAEATYVCDYILGGELDGSSSTKEEFMKKFKNAVSQGFD 300

Query: 301 PDLDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDVLQERQD 360
           PD DLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICD  QERQD
Sbjct: 301 PDTDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQD 360

Query: 361 AMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEE---------DEQRIGPGNRISYKLNHG 400
           AMYKLVEGK+DLMLVVGGWNSSNTSHLQEIAEE          EQRIGPGNRI+YKLNHG
Sbjct: 361 AMYKLVEGKVDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHG 420

[Close]
BLAST of PI0001348 vs. ExPASy TrEMBL
Match: K7NBR0 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Siraitia grosvenorii OX=190515 PE=2 SV=1)

HSP 1 Score: 672.5 bits (1734), Expect = 1.1e-189
Identity = 351/467 (75.16%), Postives = 383/467 (82.01%), Query Frame = 0 

Query: 1   MAFSMQLYRLPTRTDAALPENFTAVRSLRYRKPMSVRCDGDSSSSSSSSSA----AVDSN 60
           MAFS+QLYRL TRTDAALPENF+ +R++RYRKP+SVRCDGDSSSSSSSSS+    A+DSN
Sbjct: 1   MAFSLQLYRLSTRTDAALPENFSGIRAVRYRKPLSVRCDGDSSSSSSSSSSSSSVAMDSN 60

Query: 61  FDAKVFRRNLTKSDNYNRKGFGYKDETLALMNR--------TLKENGFEYTWGDVTVKLA 120
           FDAKVFR+NLT+S+NYNRKGFGYKDETLALMNR        TLKE+ +EYTWGDVTVKLA
Sbjct: 61  FDAKVFRKNLTRSENYNRKGFGYKDETLALMNREYTSDIIKTLKEHEYEYTWGDVTVKLA 120

Query: 121 EAYGFCWGVERAVQIAYEARKQFPEEKIWLTNEIIHNPTVNKRLEEMEVQNIPLDEGRKQ 180
           EAYGFCWGVERAVQIAYEARKQFP+EKIW+TNEIIHNPTVNKRLEEMEV+NIPL+EG+KQ
Sbjct: 121 EAYGFCWGVERAVQIAYEARKQFPDEKIWITNEIIHNPTVNKRLEEMEVENIPLEEGKKQ 180

Query: 181 FEVVDKGDVA---------------------IVDTTCPWVSKVWNVVTSTKKGYYTSIIH 240
           FEVV+KGDV                      IVDTTCPWVSKVWNVV   KKG +TSIIH
Sbjct: 181 FEVVNKGDVVILPAFGAAVDEMLTLSEKNVQIVDTTCPWVSKVWNVVEKHKKGDFTSIIH 240

Query: 241 GKYAHEETVATASFAGKYIIVKNMAEDS-------------------------KCSLGR- 300
           GKYAHEETVATASFAGKYIIVKNMAE +                         K ++ + 
Sbjct: 241 GKYAHEETVATASFAGKYIIVKNMAEATYVCDYILGGELDGSSPTKEEFMKKFKNAVSKG 300

Query: 301 FDPDLDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDVLQER 360
           FDPD+DL+KVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHF+SFNTICD  QER
Sbjct: 301 FDPDIDLVKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFISFNTICDATQER 360

Query: 361 QDAMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAE---------EDEQRIGPGNRISYKLN 400
           QDAMYKLVEGKLDLMLVVGGWNSSNTSHLQ+IAE         + EQRIGPGN+I+YKLN
Sbjct: 361 QDAMYKLVEGKLDLMLVVGGWNSSNTSHLQQIAEDRGIPSYWVDSEQRIGPGNKIAYKLN 420

[Close]
BLAST of PI0001348 vs. NCBI nr
Match: XP_004137008.2 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic [Cucumis sativus])

HSP 1 Score: 709.5 bits (1830), Expect = 1.6e-200
Identity = 377/465 (81.08%), Postives = 384/465 (82.58%), Query Frame = 0 

Query: 1   MAFSMQLYRLPTRTDAALPENFTAVRSLRYRKPMSVRCDGD--SSSSSSSSSAAVDSNFD 60
           MAFSMQLYRLPTRTDAA+PENFTAVRSLRYRKPMSVRCDGD  SSSSSSSSSAAVDSNFD
Sbjct: 1   MAFSMQLYRLPTRTDAAMPENFTAVRSLRYRKPMSVRCDGDSSSSSSSSSSSAAVDSNFD 60

Query: 61  AKVFRRNLTKSDNYNRKGFGYKDETLALMNR--------TLKENGFEYTWGDVTVKLAEA 120
           AKVFRRNLTKSDNYNRKGFGYKDETLALMNR        TLKENGFEYTWGDVTVKLAEA
Sbjct: 61  AKVFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEA 120

Query: 121 YGFCWGVERAVQIAYEARKQFPEEKIWLTNEIIHNPTVNKRLEEMEVQNIPLDEGRKQFE 180
           YGFCWGVERAVQIAYEARKQFP+EKIWLTNEIIHNPTVNKRLEEMEVQNIPL++GRKQFE
Sbjct: 121 YGFCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEDGRKQFE 180

Query: 181 VVDKGDVA---------------------IVDTTCPWVSKVWNVVTSTKKGYYTSIIHGK 240
           VVDKGDV                      IVDTTCPWVSKVWNVV   KKG YTSIIHGK
Sbjct: 181 VVDKGDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGK 240

Query: 241 YAHEETVATASFAGKYIIVKNMAE-------------DSKCSLGR-------------FD 300
           YAHEETVATASFAG +IIVKNMAE             D   S                FD
Sbjct: 241 YAHEETVATASFAGTFIIVKNMAEAIYVCDYILGGELDGSSSTKEEFMKKFKNAVSKGFD 300

Query: 301 PDLDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDVLQERQD 360
           PD+DLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICD  QERQD
Sbjct: 301 PDVDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQD 360

Query: 361 AMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEE---------DEQRIGPGNRISYKLNHG 400
           AMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEE          EQRIGPGNRI+YKLNHG
Sbjct: 361 AMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHG 420

[Close]
BLAST of PI0001348 vs. NCBI nr
Match: KAE8645891.1 (hypothetical protein Csa_017704 [Cucumis sativus])

HSP 1 Score: 709.5 bits (1830), Expect = 1.6e-200
Identity = 377/465 (81.08%), Postives = 384/465 (82.58%), Query Frame = 0 

Query: 1   MAFSMQLYRLPTRTDAALPENFTAVRSLRYRKPMSVRCDGD--SSSSSSSSSAAVDSNFD 60
           MAFSMQLYRLPTRTDAA+PENFTAVRSLRYRKPMSVRCDGD  SSSSSSSSSAAVDSNFD
Sbjct: 66  MAFSMQLYRLPTRTDAAMPENFTAVRSLRYRKPMSVRCDGDSSSSSSSSSSSAAVDSNFD 125

Query: 61  AKVFRRNLTKSDNYNRKGFGYKDETLALMNR--------TLKENGFEYTWGDVTVKLAEA 120
           AKVFRRNLTKSDNYNRKGFGYKDETLALMNR        TLKENGFEYTWGDVTVKLAEA
Sbjct: 126 AKVFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEA 185

Query: 121 YGFCWGVERAVQIAYEARKQFPEEKIWLTNEIIHNPTVNKRLEEMEVQNIPLDEGRKQFE 180
           YGFCWGVERAVQIAYEARKQFP+EKIWLTNEIIHNPTVNKRLEEMEVQNIPL++GRKQFE
Sbjct: 186 YGFCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEDGRKQFE 245

Query: 181 VVDKGDVA---------------------IVDTTCPWVSKVWNVVTSTKKGYYTSIIHGK 240
           VVDKGDV                      IVDTTCPWVSKVWNVV   KKG YTSIIHGK
Sbjct: 246 VVDKGDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGK 305

Query: 241 YAHEETVATASFAGKYIIVKNMAE-------------DSKCSLGR-------------FD 300
           YAHEETVATASFAG +IIVKNMAE             D   S                FD
Sbjct: 306 YAHEETVATASFAGTFIIVKNMAEAIYVCDYILGGELDGSSSTKEEFMKKFKNAVSKGFD 365

Query: 301 PDLDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDVLQERQD 360
           PD+DLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICD  QERQD
Sbjct: 366 PDVDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQD 425

Query: 361 AMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEE---------DEQRIGPGNRISYKLNHG 400
           AMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEE          EQRIGPGNRI+YKLNHG
Sbjct: 426 AMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHG 485

[Close]
BLAST of PI0001348 vs. NCBI nr
Match: XP_008454877.1 (PREDICTED: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like [Cucumis melo] >KAA0031252.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase [Cucumis melo var. makuwa] >TYK06703.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase [Cucumis melo var. makuwa])

HSP 1 Score: 704.5 bits (1817), Expect = 5.2e-199
Identity = 375/463 (80.99%), Postives = 381/463 (82.29%), Query Frame = 0 

Query: 1   MAFSMQLYRLPTRTDAALPENFTAVRSLRYRKPMSVRCDGDSSSSSSSSSAAVDSNFDAK 60
           MAFSMQLYRLPTRTDAALPEN +AVRSLRYRKPMSVRCDGD   SSSSSSAAVDS+FDAK
Sbjct: 1   MAFSMQLYRLPTRTDAALPENSSAVRSLRYRKPMSVRCDGD---SSSSSSAAVDSDFDAK 60

Query: 61  VFRRNLTKSDNYNRKGFGYKDETLALMNR--------TLKENGFEYTWGDVTVKLAEAYG 120
           VFRRNLTKSDNYNRKGFGYKDETLALMNR        TLKENGFEYTWGDVTVKLAEAYG
Sbjct: 61  VFRRNLTKSDNYNRKGFGYKDETLALMNREYTSDIIKTLKENGFEYTWGDVTVKLAEAYG 120

Query: 121 FCWGVERAVQIAYEARKQFPEEKIWLTNEIIHNPTVNKRLEEMEVQNIPLDEGRKQFEVV 180
           FCWGVERAVQIAYEARKQFP+EKIWLTNEIIHNPTVNKRLEEMEVQNIPL+EGRKQFEVV
Sbjct: 121 FCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEEGRKQFEVV 180

Query: 181 DKGDVA---------------------IVDTTCPWVSKVWNVVTSTKKGYYTSIIHGKYA 240
           DKGDV                      IVDTTCPWVSKVWNVV   KKG YTSIIHGKYA
Sbjct: 181 DKGDVVILPAFGAAVDEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGKYA 240

Query: 241 HEETVATASFAGKYIIVKNMAE-------------DSKCSLGR-------------FDPD 300
           HEETVATASFAGKYIIVKNMAE             D   S                FDPD
Sbjct: 241 HEETVATASFAGKYIIVKNMAEAMYVCDYILGGELDGSSSTKEEFMKKFKNAVSEGFDPD 300

Query: 301 LDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDVLQERQDAM 360
           +DLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICD  QERQDAM
Sbjct: 301 VDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQDAM 360

Query: 361 YKLVEGKLDLMLVVGGWNSSNTSHLQEIAEE---------DEQRIGPGNRISYKLNHGEL 400
           YKLVEGKLDLMLVVGGWNSSNTSHLQEIAEE          EQRIGPGNRI+YKLNHGEL
Sbjct: 361 YKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHGEL 420

[Close]
BLAST of PI0001348 vs. NCBI nr
Match: XP_038888882.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic [Benincasa hispida])

HSP 1 Score: 689.1 bits (1777), Expect = 2.3e-194
Identity = 365/463 (78.83%), Postives = 376/463 (81.21%), Query Frame = 0 

Query: 1   MAFSMQLYRLPTRTDAALPENFTAVRSLRYRKPMSVRCDGDSSSSSSSSSAAVDSNFDAK 60
           MAFSMQLYRLPTRTDA LPENF  +RS+RYRKP+SVRCDGD     SSSSAAVDS+FDAK
Sbjct: 1   MAFSMQLYRLPTRTDALLPENFATLRSVRYRKPLSVRCDGD-----SSSSAAVDSDFDAK 60

Query: 61  VFRRNLTKSDNYNRKGFGYKDETLALMNR--------TLKENGFEYTWGDVTVKLAEAYG 120
           VFRRNLTKS+NYNR+GFGYKDETLALMNR        TLKENGFEYTWG+VTVKLAEAYG
Sbjct: 61  VFRRNLTKSENYNRRGFGYKDETLALMNREYTSDVIKTLKENGFEYTWGNVTVKLAEAYG 120

Query: 121 FCWGVERAVQIAYEARKQFPEEKIWLTNEIIHNPTVNKRLEEMEVQNIPLDEGRKQFEVV 180
           FCWGVERAVQIAYEARKQFP+EKIWLTNEIIHNPTVNKRLEEMEVQNIPL+EGRKQFEVV
Sbjct: 121 FCWGVERAVQIAYEARKQFPDEKIWLTNEIIHNPTVNKRLEEMEVQNIPLEEGRKQFEVV 180

Query: 181 DKGDVA---------------------IVDTTCPWVSKVWNVVTSTKKGYYTSIIHGKYA 240
           DKGDV                      IVDTTCPWVSKVWNVV   KKG YTSIIHGKYA
Sbjct: 181 DKGDVVILPAFGAAVEEMLTLSEKKVQIVDTTCPWVSKVWNVVDKHKKGDYTSIIHGKYA 240

Query: 241 HEETVATASFAGKYIIVKNMAE-------------DSKCSLGR-------------FDPD 300
           HEETVATASFAGKYIIVKNMAE             D   S                FDPD
Sbjct: 241 HEETVATASFAGKYIIVKNMAEAMYVCDYILGGELDGSSSTKEEFMKKFKNAVSEGFDPD 300

Query: 301 LDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDVLQERQDAM 360
           +DLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICD  QERQDAM
Sbjct: 301 IDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQDAM 360

Query: 361 YKLVEGKLDLMLVVGGWNSSNTSHLQEIAEE---------DEQRIGPGNRISYKLNHGEL 400
           YKLVEGKLDLMLVVGGWNSSNTSHLQEIAEE          EQRIGPGNRI+YKLNHGEL
Sbjct: 361 YKLVEGKLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHGEL 420

[Close]
BLAST of PI0001348 vs. NCBI nr
Match: XP_022972347.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic [Cucurbita maxima])

HSP 1 Score: 672.9 bits (1735), Expect = 1.7e-189
Identity = 356/465 (76.56%), Postives = 376/465 (80.86%), Query Frame = 0 

Query: 1   MAFSMQLYRLPTRTDAALPENFTAVRSLRYRKPMSVRCDGDS--SSSSSSSSAAVDSNFD 60
           MAFSMQLYRL TRTDAALPE+F  VRS+R RKP+SVRCDGDS  S+SSSSSS AVDSNFD
Sbjct: 1   MAFSMQLYRLATRTDAALPESFPGVRSVRCRKPLSVRCDGDSSASASSSSSSVAVDSNFD 60

Query: 61  AKVFRRNLTKSDNYNRKGFGYKDETLALMN--------RTLKENGFEYTWGDVTVKLAEA 120
           AKVFRRNL+KS+NYNR+GFG+KDETLALMN        +TLKENGFEYTWGDVTVKLAEA
Sbjct: 61  AKVFRRNLSKSENYNRRGFGHKDETLALMNSEYTSDIIKTLKENGFEYTWGDVTVKLAEA 120

Query: 121 YGFCWGVERAVQIAYEARKQFPEEKIWLTNEIIHNPTVNKRLEEMEVQNIPLDEGRKQFE 180
           YGFCWGVERAVQIAYEARKQFP+EK+WLTNEIIHNPTVNKRLEEMEVQNIPL++G+KQFE
Sbjct: 121 YGFCWGVERAVQIAYEARKQFPDEKLWLTNEIIHNPTVNKRLEEMEVQNIPLEDGKKQFE 180

Query: 181 VVDKGDVA---------------------IVDTTCPWVSKVWNVVTSTKKGYYTSIIHGK 240
           VV KGDV                      IVDTTCPWVSKVWNVV   KKG +TSIIHGK
Sbjct: 181 VVGKGDVVILPAFGAGVDEMLTLSEKKVQIVDTTCPWVSKVWNVVEKHKKGEFTSIIHGK 240

Query: 241 YAHEETVATASFAGKYIIVKNMAE-------------DSKCSLGR-------------FD 300
           Y+HEETVATASFAGKYIIVKNMAE             D   S                FD
Sbjct: 241 YSHEETVATASFAGKYIIVKNMAEATYVCDYILGGELDGSSSTKEEFMKKFKNAVSQGFD 300

Query: 301 PDLDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDVLQERQD 360
           PD DLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICD  QERQD
Sbjct: 301 PDTDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDATQERQD 360

Query: 361 AMYKLVEGKLDLMLVVGGWNSSNTSHLQEIAEE---------DEQRIGPGNRISYKLNHG 400
           AMYKLVEGK+DLMLVVGGWNSSNTSHLQEIAEE          EQRIGPGNRI+YKLNHG
Sbjct: 361 AMYKLVEGKVDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHG 420

[Close]
BLAST of PI0001348 vs. TAIR 10
Match: AT4G34350.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase )

HSP 1 Score: 559.3 bits (1440), Expect = 2.5e-159
Identity = 296/466 (63.52%), Postives = 349/466 (74.89%), Query Frame = 0 

Query: 1   MAFSMQLYRLPTRTDAALPEN-FTAVRSLRYRKPMSVRCDGDSSSSSSSSSAAVDSNFDA 60
           MA ++Q  RL  R D  + EN  +   SLR RK +SVRC      ++ S S  +DS+FDA
Sbjct: 1   MAVALQFSRLCVRPDTFVRENHLSGSGSLRRRKALSVRC-SSGDENAPSPSVVMDSDFDA 60

Query: 61  KVFRRNLTKSDNYNRKGFGYKDETLALMNR--------TLKENGFEYTWGDVTVKLAEAY 120
           KVFR+NLT+SDNYNRKGFG+K+ETL LMNR        TLK NG+ Y+WGDVTVKLA+AY
Sbjct: 61  KVFRKNLTRSDNYNRKGFGHKEETLKLMNREYTSDILETLKTNGYTYSWGDVTVKLAKAY 120

Query: 121 GFCWGVERAVQIAYEARKQFPEEKIWLTNEIIHNPTVNKRLEEMEVQNIPLDEGRKQFEV 180
           GFCWGVERAVQIAYEARKQFPEE++W+TNEIIHNPTVNKRLE+M+V+ IP+++ +KQF+V
Sbjct: 121 GFCWGVERAVQIAYEARKQFPEERLWITNEIIHNPTVNKRLEDMDVKIIPVEDSKKQFDV 180

Query: 181 VDKGDVA---------------------IVDTTCPWVSKVWNVVTSTKKGYYTSIIHGKY 240
           V+K DV                      IVDTTCPWV+KVWN V   KKG YTS+IHGKY
Sbjct: 181 VEKDDVVILPAFGAGVDEMYVLNDKKVQIVDTTCPWVTKVWNTVEKHKKGEYTSVIHGKY 240

Query: 241 AHEETVATASFAGKYIIVKNMAEDS-------------------------KCSLGR-FDP 300
            HEET+ATASFAGKYIIVKNM E +                         K ++ + FDP
Sbjct: 241 NHEETIATASFAGKYIIVKNMKEANYVCDYILGGQYDGSSSTKEEFMEKFKYAISKGFDP 300

Query: 301 DLDLLKVGIANQTTMLKGETEEIGKLVERTMMRKHGVENVNEHFLSFNTICDVLQERQDA 360
           D DL+KVGIANQTTMLKGETEEIG+L+E TMMRK+GVENV+ HF+SFNTICD  QERQDA
Sbjct: 301 DNDLVKVGIANQTTMLKGETEEIGRLLETTMMRKYGVENVSGHFISFNTICDATQERQDA 360

Query: 361 MYKLVEGKLDLMLVVGGWNSSNTSHLQEIAE---------EDEQRIGPGNRISYKLNHGE 402
           +Y+LVE K+DLMLVVGGWNSSNTSHLQEI+E         + E+RIGPGN+I+YKL++GE
Sbjct: 361 IYELVEEKIDLMLVVGGWNSSNTSHLQEISEARGIPSYWIDSEKRIGPGNKIAYKLHYGE 420

The following BLAST results are available for this feature:
BLAST of PI0001348 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q94B353.6e-15863.524-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Arabidopsis... [more]
Q6AVG62.6e-15667.904-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Oryza sativ... [more]
Q31S642.1e-11352.904-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Synechococcus elongatus (s... [more]
Q5N2492.1e-11352.904-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Synechococcus sp. (strain ... [more]
B8HWD34.6e-11354.294-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Cyanothece sp. (strain PCC... [more]
BLAST of PI0001348 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0K3D87.9e-20181.08Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G075680 PE=3 SV=1[more]
A0A5A7SJM12.5e-19980.994-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Cucumis melo var. makuwa O... [more]
A0A1S3BZP52.5e-19980.994-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like OS=Cucumi... [more]
A0A6J1I4K48.2e-19076.564-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Cucurbita m... [more]
K7NBR01.1e-18975.164-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Siraitia grosvenorii OX=19... [more]
BLAST of PI0001348 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004137008.21.6e-20081.084-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic [Cucumis sativ... [more]
KAE8645891.11.6e-20081.08hypothetical protein Csa_017704 [Cucumis sativus][more]
XP_008454877.15.2e-19980.99PREDICTED: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-lik... [more]
XP_038888882.12.3e-19478.834-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic [Benincasa his... [more]
XP_022972347.11.7e-18976.564-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic [Cucurbita max... [more]
BLAST of PI0001348 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G34350.12.5e-15963.524-hydroxy-3-methylbut-2-enyl diphosphate reductase [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR0034514-hydroxy-3-methylbut-2-enyl diphosphate reductasePFAMPF02401LYTBcoord: 105..387
e-value: 1.7E-67
score: 227.5
IPR0034514-hydroxy-3-methylbut-2-enyl diphosphate reductaseCDDcd13944lytB_ispHcoord: 105..387
e-value: 4.3337E-83
score: 253.487
NoneNo IPR availableGENE3D3.40.50.11270coord: 114..183
e-value: 1.2E-13
score: 52.9
NoneNo IPR availableGENE3D3.40.1010.20coord: 292..404
e-value: 7.6E-28
score: 98.5
NoneNo IPR availableGENE3D3.40.1010.20coord: 184..291
e-value: 6.1E-17
score: 63.5
NoneNo IPR availablePANTHERPTHR316194-HYDROXY-3-METHYLBUT-2-ENYL DIPHOSPHATE REDUCTASE, CHLOROPLASTICcoord: 242..399
coord: 1..233

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0001348.1PI0001348.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0050992 dimethylallyl diphosphate biosynthetic process
biological_process GO:0019288 isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
molecular_function GO:0051745 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0046872 metal ion binding