PI0001191 (gene) Melon (PI 482460) v1

BLAST of PI0001191 vs. ExPASy Swiss-Prot
Match: O81103 (Polyphenol oxidase latent form, chloroplastic OS=Prunus armeniaca OX=36596 GN=PPO PE=1 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 3.0e-112
Identity = 248/591 (41.96%), Postives = 338/591 (57.19%), Query Frame = 0

Query: 1   MASLSSLLSVSPYS---GRKIQSSIIPSNKRH-GVINYVSCKASNNGED----------- 60
           M + SSL+S + +S     K Q S+   +K +    + +SCKA+NN              
Sbjct: 10  MGTYSSLISTNSFSTFLPNKSQLSLSGKSKHYVARRSSISCKATNNNNSNNQNEQQEESS 69

Query: 61  -FLSKFDRRDVLFGLGGLYGVTTLSGNDIALATDWSLSPDVHKCKPAN-PPAGK--MDCC 120
             L K DRR++L GLGGLYG TTL     A A D    PD+  CKPA   P G   + CC
Sbjct: 70  RLLGKLDRRNILIGLGGLYGATTLDRKPFAFA-DPIAPPDLTTCKPAEITPGGSETVPCC 129

Query: 121 PPKINEIIEFTPPVLPTPRVRPAVHLMSPDQIADFEKGIQMMKNLPEDDPCSFKHQAKVH 180
           PP   +I  F P +    R  PA H ++ + +A F+K    M+ LP+DDP S   QAKVH
Sbjct: 130 PPVTTKIKTFKPDLSIPLRTSPAAHQVTDEYLAKFKKAQAAMRALPDDDPRSMVQQAKVH 189

Query: 181 CAYCDNAYKEIDST-VDFQVHSNALFFPFHRAYLYFFERILCHLLEKPDFAIPFWNWDAP 240
           CAYC+ AY ++  T  D QVH + LFFPFHR YLYF+ERIL  L++ P FA+P+WNWD+P
Sbjct: 190 CAYCNGAYPQVGFTDNDIQVHFSWLFFPFHRMYLYFYERILGKLIDDPTFALPYWNWDSP 249

Query: 241 PGMYMPEIYTKPSSSLYDENRNECHQPPKLLNLNYGGKKLK-DPNIIVDYNLRWMNDQM- 300
            G  +P+IYT  SS LYD+ RN  HQPP L++L+YGGK    D    +D NL  M  QM 
Sbjct: 250 VGFPIPDIYTDTSSPLYDQYRNADHQPPVLVDLSYGGKDDDVDEQTRIDENLAIMYRQMV 309

Query: 301 -NVETAKEFLGQVMKRGSCLEDGS--GSIEMSPHNNIHIWVGDPEAKITGEDMGHFHSAG 360
              +T   F G   + G+ L  G   G+IE  PHNNIHIWVGDP ++   EDMG+F+SAG
Sbjct: 310 SGAKTPDLFFGHAYRAGN-LNTGKYPGTIENMPHNNIHIWVGDP-SQTHQEDMGNFYSAG 369

Query: 361 KDPLFYAHHANVDRMWNIWQKMKDRPRDHTDPDWLNSSFVLYDENR-------------- 420
           +DPLFYAHHANVDRMWNIW+ +  + +D TD DWL++ F+ YDEN               
Sbjct: 370 RDPLFYAHHANVDRMWNIWKTLGGKRKDITDTDWLDAEFLFYDENAELVRVKVRDSLEPE 429

Query: 421 -------KAVPIPWLYTPAINR--PNLSASRLRNLPPTTDFPQILSSDITTVVDRPE--V 480
                  + V +PWL+T    R   N + +++     T+ FP  L    T  V RP+   
Sbjct: 430 KQLRYNYEPVSLPWLFTKPTARKTKNKTKAKVAATQLTSKFPATLVEVTTVEVARPKPRK 489

Query: 481 ASPEERINKNEVLVLEDVEFDPTLPLHFDVLLNVGDEGEDV-RAGHIEFVGTFTNVPHGR 539
            S +E++++ E+L+++D+EF+ T  + FDV +N  D+ E + R    EF G+F +VP G+
Sbjct: 490 RSKKEKVDEEELLIIKDIEFEGTEAVKFDVFIN--DDAESLSRRDKSEFAGSFVHVPQGK 549

BLAST of PI0001191 vs. ExPASy Swiss-Prot
Match: P43309 (Polyphenol oxidase, chloroplastic OS=Malus domestica OX=3750 PE=1 SV=1)

HSP 1 Score: 393.3 bits (1009), Expect = 4.5e-108
Identity = 231/578 (39.97%), Postives = 329/578 (56.92%), Query Frame = 0

Query: 10  VSPYSGRKIQSSIIPSNKRHGVINYVSCKASNN--GEDFLSKFDRRDVLFGLGGLYGVTT 69
           +SP+S    Q S++   KR      VSCKA+NN   +   SK DRR+VL GLGGLYGV  
Sbjct: 22  LSPFSQNNSQVSLLTKPKR-SFARKVSCKATNNDQNDQAQSKLDRRNVLLGLGGLYGVAG 81

Query: 70  LSGNDIALATDWSLSPDVHKCKPANPPAGKM--DCCPPKINEIIEFTPPVLPTPRVRPAV 129
           +  +  A A   +  PDV KC PA+ P G +  +CCPP   +II+F  P     R+RP  
Sbjct: 82  MGTDPFAFAKPIA-PPDVSKCGPADLPQGAVPTNCCPPPSTKIIDFKLPAPAKLRIRPPA 141

Query: 130 HLMSPDQIADFEKGIQMMKNLPEDDPCSFKHQAKVHCAYCDNAYKEID-STVDFQVHSNA 189
           H +       + K +++MK LP+DDP SFK QA VHCAYCD AY ++    ++ Q+H++ 
Sbjct: 142 HAVDQAYRDKYYKAMELMKALPDDDPRSFKQQAAVHCAYCDGAYDQVGFPELELQIHNSW 201

Query: 190 LFFPFHRAYLYFFERILCHLLEKPDFAIPFWNWDAPPGMYMPEIYTKPSSSLYDENRNEC 249
           LFFPFHR YLYFFE+IL  L+  P FA+PFWNWD+P GM +P IY  P S LYD+ R+  
Sbjct: 202 LFFPFHRYYLYFFEKILGKLINDPTFALPFWNWDSPAGMPLPAIYADPKSPLYDKLRSAN 261

Query: 250 HQPPKLLNLNYGGKKLK-DPNIIVDYNLRWMNDQM--NVETAKEFLGQVMKRGSCLEDGS 309
           HQPP L++L+Y G +        ++ NL+ M  QM  N + AK F G   + G   + G 
Sbjct: 262 HQPPTLVDLDYNGTEDNVSKETTINANLKIMYRQMVSNSKNAKLFFGNPYRAGDEPDPGG 321

Query: 310 GSIEMSPHNNIHIWVGDPEAKITGEDMGHFHSAGKDPLFYAHHANVDRMWNIWQKMKDRP 369
           GSIE +PH  +H+W GD   +   EDMG+F+SAG+DP+F+AHH+NVDRMW+IW+ +  + 
Sbjct: 322 GSIEGTPHAPVHLWTGD-NTQPNFEDMGNFYSAGRDPIFFAHHSNVDRMWSIWKTLGGKR 381

Query: 370 RDHTDPDWLNSSFVLYDENRKA--------------------VPIPWLYT-PAINRPNLS 429
            D TD DWL+S F+ Y+EN +                     V IPWL + P   R  ++
Sbjct: 382 TDLTDSDWLDSGFLFYNENAELVRVKVRDCLETKNLGYVYQDVDIPWLSSKPTPRRAKVA 441

Query: 430 ASRL--------------RNLPPTTDFPQILSSDITTVVDRP--EVASPEERINKNEVLV 489
            S++                +   T+FP  L S I+TVV RP  +  S + + ++ E+LV
Sbjct: 442 LSKVAKKLGVAHAAVASSSKVVAGTEFPISLGSKISTVVKRPKQKKRSKKAKEDEEEILV 501

Query: 490 LEDVEFDPTLPLHFDVLLNVGDEGEDVRAG--HIEFVGTFTNVPHG--RMTHHITSLHFV 539
           +E +EFD  + + FDV +N   + +D+ +G    EF G+F +VPH         T L   
Sbjct: 502 IEGIEFDRDVAVKFDVYVN---DVDDLPSGPDKTEFAGSFVSVPHSHKHKKKMNTILRLG 561

BLAST of PI0001191 vs. ExPASy Swiss-Prot
Match: P43311 (Polyphenol oxidase, chloroplastic OS=Vitis vinifera OX=29760 PE=1 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 5.0e-107
Identity = 240/580 (41.38%), Postives = 330/580 (56.90%), Query Frame = 0

Query: 5   SSLLSVSPYSGRKIQSSIIPSNKRHGVINYVSCKASNNGEDFLSKFDRRDVLFGLGGLYG 64
           +S + V+    R+   S +  N  +G  N  S   S+  E    K DRR+VL G+GGLYG
Sbjct: 32  ASHVPVARNRSRRFAPSKVSCNSANGDPN--SDSTSDVRETSSGKLDRRNVLLGIGGLYG 91

Query: 65  VTTLSGNDIALATDWSL-SPDVHKCKPANPPAG--KMDCCPPKINEIIEFTPPVLPTP-R 124
                G    LA    + +PD+ KC  A  P G    +CCPP   +II+F  P   +P R
Sbjct: 92  AAGGLGATKPLAFGAPIQAPDISKCGTATVPDGVTPTNCCPPVTTKIIDFQLPSSGSPMR 151

Query: 125 VRPAVHLMSPDQIADFEKGIQMMKNLPEDDPCSFKHQAKVHCAYCDNAYKEIDST-VDFQ 184
            RPA HL+S + +A ++K I++ K LP+DDP SFK QA VHC YC  AY ++  T ++ Q
Sbjct: 152 TRPAAHLVSKEYLAKYKKAIELQKALPDDDPRSFKQQANVHCTYCQGAYDQVGYTDLELQ 211

Query: 185 VHSNALFFPFHRAYLYFFERILCHLLEKPDFAIPFWNWDAPPGMYMPEIYTKPSSSLYDE 244
           VH++ LF PFHR YLYF ERIL  L++ P FA+P+W WD P GMYMP IY    SSLYDE
Sbjct: 212 VHASWLFLPFHRYYLYFNERILAKLIDDPTFALPYWAWDNPDGMYMPTIYASSPSSLYDE 271

Query: 245 NRNECHQPPKLLNLNYGGKK--LKDPNIIVDYNLRWMNDQM--NVETAKEFLGQVMKRGS 304
            RN  H PP +++L+Y G +  + D  +  D NL  M  Q+     T K FLG   + G 
Sbjct: 272 KRNAKHLPPTVIDLDYDGTEPTIPDDELKTD-NLAIMYKQIVSGATTPKLFLGYPYRAGD 331

Query: 305 CLEDGSGSIEMSPHNNIHIWVGDPEAKITGEDMGHFHSAGKDPLFYAHHANVDRMWNIWQ 364
            ++ G+G++E +PHN +H W G   A    EDMG+F++AG+DP+F+ HHANVDRMWNIW+
Sbjct: 332 AIDPGAGTLEHAPHNIVHKWTG--LADKPSEDMGNFYTAGRDPIFFGHHANVDRMWNIWK 391

Query: 365 KMKDRPR-DHTDPDWLNSSFVLYDENRKAV--------------------PIPWL--YTP 424
            +  + R D TD DWL+++FV YDEN++ V                    PIPWL   T 
Sbjct: 392 TIGGKNRKDFTDTDWLDATFVFYDENKQLVKVKVSDCVDTSKLRYQYQDIPIPWLPKNTK 451

Query: 425 AINRPNLSAS-----RLRNLPPTT-----DFPQILSSDITTVVDRPEVA-SPEERINKNE 484
           A  +    +S     +   LP TT     DFP+ L+S I   V RP+ + S +E+ ++ E
Sbjct: 452 AKAKTTTKSSKSGVAKAAELPKTTISSIGDFPKALNSVIRVEVPRPKKSRSKKEKEDEEE 511

Query: 485 VLVLEDVEFDPTLPLHFDVLLNVGDEGEDV-RAGHIEFVGTFTNVPHGRMTH--HITSLH 539
           VL+++ +E D    + FDV +N  DE   V R  + EF G+F NVPH  M      T+L 
Sbjct: 512 VLLIKGIELDRENFVKFDVYIN--DEDYSVSRPKNSEFAGSFVNVPHKHMKEMKTKTNLR 571

BLAST of PI0001191 vs. ExPASy Swiss-Prot
Match: Q06215 (Polyphenol oxidase A1, chloroplastic OS=Vicia faba OX=3906 PE=1 SV=1)

HSP 1 Score: 384.0 bits (985), Expect = 2.8e-105
Identity = 236/595 (39.66%), Postives = 333/595 (55.97%), Query Frame = 0

Query: 9   SVSPYSGRKIQSSIIPSNKRHGVINYVSCKASNN-----GEDFLSKF--DRRDVLFGLGG 68
           SV P+  ++ QSS +   KR      V+C ++NN      E  LS     RR+VL GLGG
Sbjct: 25  SVYPFLQKQHQSSKLRKPKRQ-----VTCSSNNNQNNPKEEQELSNIVGHRRNVLIGLGG 84

Query: 69  LYGVTTLSGNDIALATDWSLSPDVHKC-KPANPPAG----KMDCCPPKINEIIEFTPPVL 128
           +YG  TL+ N  ALA+  S  PD+ KC  P++ P+G     ++CCPP   +I +F  P  
Sbjct: 85  IYG--TLATNPSALASPIS-PPDLSKCVPPSDLPSGTTPPNINCCPPYSTKITDFKFPSN 144

Query: 129 PTPRVRPAVHLMSPDQIADFEKGIQMMKNLPEDDPCSFKHQAKVHCAYCDNAYKEID-ST 188
              RVR A HL+  + +  ++K  ++MK LP +DP +F  QA +HCAYCD AY +I    
Sbjct: 145 QPLRVRQAAHLVDNEFLEKYKKATELMKALPSNDPRNFTQQANIHCAYCDGAYSQIGFPD 204

Query: 189 VDFQVHSNALFFPFHRAYLYFFERILCHLLEKPDFAIPFWNWDAPPGMYMPEIYTKPSSS 248
           +  QVH + LFFPFHR YLYF+ERIL  L+  P FA+PFWN+DAP GM +P IY   +S 
Sbjct: 205 LKLQVHGSWLFFPFHRWYLYFYERILGSLINDPTFALPFWNYDAPDGMQLPTIYADKASP 264

Query: 249 LYDENRNECHQPPKLLNLNYG--GKKLKDPNIIVDYNLRWMNDQM--NVETAKEFLGQVM 308
           LYDE RN  HQPP L++LN+   G  + D N ++  NL  M  Q+  N +T++ FLG   
Sbjct: 265 LYDELRNASHQPPTLIDLNFCDIGSDI-DRNELIKTNLSIMYRQVYSNGKTSRLFLGNPY 324

Query: 309 KRGSCLEDGSGSIEMSPHNNIHIWVGDPEAKITGEDMGHFHSAGKDPLFYAHHANVDRMW 368
           + G     G+GSIE  PH  +H W GD   +   EDMG F+SA +DP+FY+HH+NVDR+W
Sbjct: 325 RAGDAEPQGAGSIENVPHAPVHTWTGD-NTQTNIEDMGIFYSAARDPIFYSHHSNVDRLW 384

Query: 369 NIWQKMKDRPRDHTDPDWLNSSFVLYDENR--------------------KAVPIPW--- 428
            IW+ +  +  D TD DWL S F+ YDEN+                    + VPIPW   
Sbjct: 385 YIWKTLGGKKHDFTDKDWLESGFLFYDENKNLVRVNVKDSLDIDKLGYAYQDVPIPWEKA 444

Query: 429 -----------LYTPAINRPNLSAS------RLRNLPPTTDFPQILSSDITTVVDRP-EV 488
                      L    +N  NL  S      R ++      FP +L++ ++ +V RP ++
Sbjct: 445 KPVPRRTKVQKLVEVEVNDGNLRKSPTIFLVRQQSPRKYVTFPLVLNNKVSAIVKRPKKL 504

Query: 489 ASPEERINKNEVLVLEDVEFDPTLPLHFDVLLNVGDEGEDVRAGHIEFVGTFTNVPHGRM 542
            S +E+  + EVLV+E +EF   + + FDV +N  DE + V AG+ EF G+F N+PH   
Sbjct: 505 RSKKEKEEEEEVLVIEGIEFYMNIAIKFDVYIN--DEDDKVGAGNTEFAGSFVNIPHSAH 564

BLAST of PI0001191 vs. ExPASy Swiss-Prot
Match: Q6UIL3 ((+)-larreatricin hydroxylase, chloroplastic OS=Larrea tridentata OX=66636 PE=1 SV=1)

HSP 1 Score: 372.5 bits (955), Expect = 8.3e-102
Identity = 237/592 (40.03%), Postives = 334/592 (56.42%), Query Frame = 0

Query: 1   MASLS--SLLSVSPYSGRKIQSSIIPSNKRHGVINY--VSCKASN------NGEDFLSKF 60
           MASLS  S L  +PYS         P + +   ++   VSCKASN      N ++     
Sbjct: 1   MASLSSQSKLLATPYS--------FPYHTKPSRVSLRRVSCKASNDNKDKPNDQEKTFSI 60

Query: 61  DRRDVLFGLGGLYGVTTL-SGNDIALATDWSLSPDV--HKCKPANPPAGKMD--CCPPKI 120
           DRR++L GLGGLYG + +   N   LA    + P V    C P    A  ++  CCPP +
Sbjct: 61  DRRNMLIGLGGLYGASNVFPSNQSTLAA--PIQPPVLPDSCHPPEDLAEGVNVLCCPPDV 120

Query: 121 NEIIEFTPPVLPTP-RVRPAVHLMSPDQIADFEKGIQMMKNLPEDDPCSFKHQAKVHCAY 180
            + I+F  P  P+  R+RPA HL  P  I  ++K +  MK LP++DP SF  QA +HCAY
Sbjct: 121 KDPIDFQMPSNPSRLRIRPAAHLADPTYIEKYKKALAAMKALPQNDPRSFYQQANIHCAY 180

Query: 181 CDNAYKEID-STVDFQVHSNALFFPFHRAYLYFFERILCHLLEKPDFAIPFWNWDAPPGM 240
           C+ AY ++    V+ QVH + LF PFHR YLYF+ERIL  L++ P FAIPFWNWDAP GM
Sbjct: 181 CNGAYDQVGFPDVNIQVHHSWLFLPFHRWYLYFYERILGSLIDDPTFAIPFWNWDAPKGM 240

Query: 241 YMPEIYTKPSSSLYDENRNECHQPPKLLNLNYGGKKLKDPN-IIVDYNLRWMNDQ-MNVE 300
           +MP ++  P+S LYD  RN  H P  +++L++   +    N   +  NL  M  Q +  +
Sbjct: 241 HMPHMFIDPNSPLYDAKRNPAHFPDTIVDLDFSSGEAPSHNPRQIGNNLSIMYKQVVRAK 300

Query: 301 TAKEFLGQVMKRGSCL-EDGSGSIEMSPHNNIHIWVGDPEAKITGEDMGHFHSAGKDPLF 360
            A+ F G+ ++ GS   E G GS+E +PH NIH+W GDP  +   E+MG+F+SAG+DPLF
Sbjct: 301 KARLFHGRPLQAGSFPDESGDGSLEGTPHGNIHLWSGDPR-QSNFENMGNFYSAGRDPLF 360

Query: 361 YAHHANVDRMWNIWQKMKDRPRDHTDPDWLNSSFVLYDENR------------------- 420
           YAHHANVDRMW IW+    R +D+   DWLN+ F+L+DEN                    
Sbjct: 361 YAHHANVDRMWYIWKNSLGR-KDYKKKDWLNAGFLLFDENAQPVRVYVRDALDERKLGYA 420

Query: 421 -KAVPIPWLYT-PAINRPNLSASRLRNLPPTT-----DFPQILSSDITTVVDRPEVA-SP 480
            + V IPW+ + P   + N   +RLR+   TT      FP  L S ++  V RP+ + S 
Sbjct: 421 YQEVDIPWINSKPKPRKANPWPNRLRSKATTTTKLINKFPLTLDSTVSFEVKRPKKSRSK 480

Query: 481 EERINKNEVLVLEDVEFDPTLPLHFDVLLNVGDEGEDVRAG-HIEFVGTFTNVPH----- 539
            E+ ++ EVLV+E ++ +P  PL FDV +N  DE ED  A    EF G+F NVPH     
Sbjct: 481 SEKEDEEEVLVIEKIKHEPQFPLKFDVYIN--DEDEDPSAADQTEFAGSFVNVPHFHRHG 540

BLAST of PI0001191 vs. ExPASy TrEMBL
Match: A0A5A7UTU0 (Polyphenol oxidase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00530 PE=3 SV=1)

HSP 1 Score: 958.4 bits (2476), Expect = 1.3e-275
Identity = 469/565 (83.01%), Postives = 500/565 (88.50%), Query Frame = 0

Query: 1   MASLSSLLSVSPYSGRKIQSSIIPSNKRHGVINYVSCKASNNGEDFLSKFDRRDVLFGLG 60
           MASLSSLLSVSPYSG KIQ S+IP NKR GV N+VSCKASNNGED ++KFDRRD+LFGLG
Sbjct: 1   MASLSSLLSVSPYSGCKIQPSVIPLNKRRGVNNHVSCKASNNGEDSVNKFDRRDMLFGLG 60

Query: 61  GLYGVTTLSGNDIALATDWSLSPDVHKCKPANPPA--GKMDCCPPKINEIIEFTPPVLPT 120
           GLYGV TLSG DIALA DWSLSPDVH CKP  P A   KMDCCPPKI EIIEFTPPV+PT
Sbjct: 61  GLYGVATLSGKDIALADDWSLSPDVHNCKPVKPSAVGKKMDCCPPKIKEIIEFTPPVVPT 120

Query: 121 PRVRPAVHLMSPDQIADFEKGIQMMKNLPEDDPCSFKHQAKVHCAYCDNAYKEIDSTVDF 180
           PRVRPAVHLM+P+QIAD EKGIQMMK LPEDDP SF HQAKVHCAYCD+AYK++DST+DF
Sbjct: 121 PRVRPAVHLMTPEQIADLEKGIQMMKMLPEDDPRSFAHQAKVHCAYCDDAYKQMDSTMDF 180

Query: 181 QVHSNALFFPFHRAYLYFFERILCHLLEKPDFAIPFWNWDAPPGMYMPEIYTKPSSSLYD 240
           QVH NALFFPFHRAYLYFFERILCHLLEKPDFAIPFWNWDAPPGM MP+IYTKPSSSLYD
Sbjct: 181 QVHENALFFPFHRAYLYFFERILCHLLEKPDFAIPFWNWDAPPGMCMPKIYTKPSSSLYD 240

Query: 241 ENRNECHQPPKLLNLNYGGKKLKDPNIIVDYNLRWMNDQMNVETAKEFLGQVMKRGSCLE 300
           ENR++CHQPPKLLNLNYGGK++KDP  IVD+NLRWMNDQM+VETAKEFLG+V+KRG+  E
Sbjct: 241 ENRDKCHQPPKLLNLNYGGKEVKDPE-IVDFNLRWMNDQMSVETAKEFLGKVIKRGNGPE 300

Query: 301 DGSGSIEMSPHNNIHIWVGDPEAKITGEDMGHFHSAGKDPLFYAHHANVDRMWNIWQKMK 360
           +GSGSIE SPHNNIHIWVGDPEA I GEDMGHF+SAG+DPLFYAHHANVDRMWNIWQKMK
Sbjct: 301 NGSGSIETSPHNNIHIWVGDPEATIHGEDMGHFYSAGRDPLFYAHHANVDRMWNIWQKMK 360

Query: 361 DRPRDHTDPDWLNSSFVLYDENRKA--------------------VPIPWLYTPAINRPN 420
           DRPRDHTD DWLNSSFVLYDENRKA                    VPIPW  TP I RP 
Sbjct: 361 DRPRDHTDMDWLNSSFVLYDENRKAVRIKVSQCLDSKILGYVYQDVPIPWKDTPPIRRPK 420

Query: 421 LSASRLRNLPPTTDFPQILSSDITTVVDRPEVASPEERINKNEVLVLEDVEFDPTLPLHF 480
              S L NLPPT DFPQILSSDITT+VDRPEVASPEERINKNEVLVLEDVE+DPTLPLHF
Sbjct: 421 QERS-LGNLPPTIDFPQILSSDITTMVDRPEVASPEERINKNEVLVLEDVEYDPTLPLHF 480

Query: 481 DVLLNVGDEGEDVRAGHIEFVGTFTNVPHGRMTHHITSLHFVITKKIRQLGLEGENKVAV 540
           DVLLNVGDEGEDVRAGHIEFVGTFTNVPHGR T+H TSLHFVITKKI+QLGLEG+ KVAV
Sbjct: 481 DVLLNVGDEGEDVRAGHIEFVGTFTNVPHGRTTYHKTSLHFVITKKIQQLGLEGDKKVAV 540

Query: 541 KIVPNVNGGDKIKIGGIKIQLEDIE 544
           KIVPNVNGGD+IKIGGIKIQLEDIE
Sbjct: 541 KIVPNVNGGDRIKIGGIKIQLEDIE 563

BLAST of PI0001191 vs. ExPASy TrEMBL
Match: A0A5A7UM24 (Polyphenol oxidase I OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold96G001410 PE=3 SV=1)

HSP 1 Score: 950.3 bits (2455), Expect = 3.6e-273
Identity = 467/565 (82.65%), Postives = 497/565 (87.96%), Query Frame = 0

Query: 1   MASLSSLLSVSPYSGRKIQSSIIPSNKRHGVINYVSCKASNNGEDFLSKFDRRDVLFGLG 60
           MASLSSLLSVSPYSG KIQ S+IP NKR GV N+VSCKASNNGED ++KFDRRD+LFGLG
Sbjct: 1   MASLSSLLSVSPYSGCKIQPSVIPLNKRRGVNNHVSCKASNNGEDSVNKFDRRDMLFGLG 60

Query: 61  GLYGVTTLSGNDIALATDWSLSPDVHKCKPANPPA--GKMDCCPPKINEIIEFTPPVLPT 120
           GLYGV TLSG DIALA DWSLSPDVH CKP  P A   KMDCCPPKI EIIEFTPPV+PT
Sbjct: 61  GLYGVATLSGKDIALADDWSLSPDVHNCKPVKPSAVGKKMDCCPPKIKEIIEFTPPVVPT 120

Query: 121 PRVRPAVHLMSPDQIADFEKGIQMMKNLPEDDPCSFKHQAKVHCAYCDNAYKEIDSTVDF 180
           PRVRPAVHLM+PDQIAD EKGIQMMK LPEDDP SF HQAKVHCAYCD+AYK++DST+DF
Sbjct: 121 PRVRPAVHLMTPDQIADLEKGIQMMKMLPEDDPRSFAHQAKVHCAYCDDAYKQMDSTMDF 180

Query: 181 QVHSNALFFPFHRAYLYFFERILCHLLEKPDFAIPFWNWDAPPGMYMPEIYTKPSSSLYD 240
           QVH NALFFPFHRAYLYFFERILCHLLEKPDFAIPFWNWDAPPGM MP+IYTKPSSSLYD
Sbjct: 181 QVHENALFFPFHRAYLYFFERILCHLLEKPDFAIPFWNWDAPPGMCMPKIYTKPSSSLYD 240

Query: 241 ENRNECHQPPKLLNLNYGGKKLKDPNIIVDYNLRWMNDQMNVETAKEFLGQVMKRGSCLE 300
           ENR+ECHQPPKLLNLNYGGKK+KD   IVD+NLRWMNDQM+VETA+EFLG+V+KRG+C E
Sbjct: 241 ENRDECHQPPKLLNLNYGGKKVKDSE-IVDFNLRWMNDQMSVETAREFLGKVIKRGNCPE 300

Query: 301 DGSGSIEMSPHNNIHIWVGDPEAKITGEDMGHFHSAGKDPLFYAHHANVDRMWNIWQKMK 360
           +GSGSIE SPHNN+H WVGDPEA I GEDMGHF+SAG+DPLFYAHHANVDRMWNIWQ MK
Sbjct: 301 NGSGSIETSPHNNLHNWVGDPEATIHGEDMGHFYSAGRDPLFYAHHANVDRMWNIWQNMK 360

Query: 361 DRPRDHTDPDWLNSSFVLYDENRKA--------------------VPIPWLYTPAINRPN 420
           DR RDHTD DWLNSSFVLYDENRKA                    VPIPW  TP I RP 
Sbjct: 361 DR-RDHTDMDWLNSSFVLYDENRKAVRIKVSQCLDSKILGYVYQDVPIPWKDTPPIRRPK 420

Query: 421 LSASRLRNLPPTTDFPQILSSDITTVVDRPEVASPEERINKNEVLVLEDVEFDPTLPLHF 480
              S L NLPPT DFPQILSSDITT+VDRPEVASPEERINKNEVLVLEDVE+DPTLPLHF
Sbjct: 421 QERS-LGNLPPTIDFPQILSSDITTMVDRPEVASPEERINKNEVLVLEDVEYDPTLPLHF 480

Query: 481 DVLLNVGDEGEDVRAGHIEFVGTFTNVPHGRMTHHITSLHFVITKKIRQLGLEGENKVAV 540
           DVLLNVGDEGEDVRAGHIEFVGTFTNVPHGR T+H TSLHFVITKKI+QLGLEG+ KVAV
Sbjct: 481 DVLLNVGDEGEDVRAGHIEFVGTFTNVPHGRTTYHKTSLHFVITKKIQQLGLEGDKKVAV 540

Query: 541 KIVPNVNGGDKIKIGGIKIQLEDIE 544
           KIVPNVNGGD+IKIGGIKIQLEDIE
Sbjct: 541 KIVPNVNGGDRIKIGGIKIQLEDIE 562

BLAST of PI0001191 vs. ExPASy TrEMBL
Match: A0A0A0LV27 (Tyrosinase_Cu-bd domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G073110 PE=3 SV=1)

HSP 1 Score: 669.1 bits (1725), Expect = 1.6e-188
Identity = 355/544 (65.26%), Postives = 373/544 (68.57%), Query Frame = 0

Query: 1   MASLSSLLSVSPYSGRKIQSSIIPSNKRHGVINYVSCKASNNGEDFLSKFDRRDVLFGLG 60
           MASLSSLLSVSPYSG KIQSSIIP NKRHGV NYVSCKASN+ ED ++KFDRRDVLFGLG
Sbjct: 1   MASLSSLLSVSPYSGCKIQSSIIPLNKRHGVNNYVSCKASNHIEDSVNKFDRRDVLFGLG 60

Query: 61  GLYGVTTLSGNDIALATDWSLSPDVHKCKPANPPAG-KMDCCPPKINEIIEFTPPVLPTP 120
           GLYGV TLSGNDI LA DWSLSPDVH CKP+ P AG KMDCCPPKIN+IIEFTPPV+PT 
Sbjct: 61  GLYGVATLSGNDIGLAADWSLSPDVHNCKPSKPSAGKKMDCCPPKINKIIEFTPPVVPTL 120

Query: 121 RVRPAVHLMSPDQIADFEKGIQMMKNLPEDDPCSFKHQAKVHCAYCDNAYKEIDSTVDFQ 180
           RVRPAVHLM+PDQIAD EKGIQMMKNLPEDDPCSF HQAKVHCAYC++AYK+        
Sbjct: 121 RVRPAVHLMTPDQIADLEKGIQMMKNLPEDDPCSFAHQAKVHCAYCNDAYKQ-------- 180

Query: 181 VHSNALFFPFHRAYLYFFERILCHLLEKPDFAIPFWNWDAPPGMYMPEIYTKPSSSLYDE 240
                                                                       
Sbjct: 181 ------------------------------------------------------------ 240

Query: 241 NRNECHQPPKLLNLNYGGKKLKDPNIIVDYNLRWMNDQMNVETAKEFLGQVMKRGSCLED 300
                                                                       
Sbjct: 241 ------------------------------------------------------------ 300

Query: 301 GSGSIEMSPHNNIHIWVGDPEAKITGEDMGHFHSAGKDPLFYAHHANVDRMWNIWQKMKD 360
               IEMSPHNNIH WVGDPEA I GE+MGHFHSAGKDP+FYAHH NVDRMWNIWQKMKD
Sbjct: 301 ----IEMSPHNNIHNWVGDPEATIQGENMGHFHSAGKDPVFYAHHTNVDRMWNIWQKMKD 360

Query: 361 RPRDHTDPDWLNSSFVLYDENRKAVPIPWLYTPAINRPNLSASRLRNLPPTTDFPQILSS 420
           +PRDHTDPDWLNSSFVLYDENRKA        P I RPN    RL NLPPT DFPQILSS
Sbjct: 361 QPRDHTDPDWLNSSFVLYDENRKA------DNPPIPRPNRWERRLGNLPPTIDFPQILSS 406

Query: 421 DITTVVDRPEVASPEERINKNEVLVLEDVEFDPTLPLHFDVLLNVGDEGEDVRAGHIEFV 480
           DITT+VDRPEVASPEERINKNEVLVLEDVEFDPTLPLHFDVLLNVGD+GEDVRAG IEFV
Sbjct: 421 DITTMVDRPEVASPEERINKNEVLVLEDVEFDPTLPLHFDVLLNVGDDGEDVRAGDIEFV 406

Query: 481 GTFTNVPHGRMTHHITSLHFVITKKIRQLGLEGENKVAVKIVPNVNGGDKIKIGGIKIQL 540
           GTFTNVPHGRMTHH TSLHFV+TKKI QLGLEG+ KVAVKIVPNVNGGD+IKIGGIKIQL
Sbjct: 481 GTFTNVPHGRMTHHKTSLHFVLTKKIVQLGLEGDKKVAVKIVPNVNGGDRIKIGGIKIQL 406

Query: 541 EDIE 544
           EDIE
Sbjct: 541 EDIE 406

BLAST of PI0001191 vs. ExPASy TrEMBL
Match: A0A5A7UM88 (Polyphenol oxidase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00520 PE=3 SV=1)

HSP 1 Score: 619.0 bits (1595), Expect = 1.9e-173
Identity = 324/568 (57.04%), Postives = 390/568 (68.66%), Query Frame = 0

Query: 1   MASLSSLLSVSPYSGRKIQSSIIPSNKRHGVINYVSCKA--SNNGEDFLSKFDRRDVLFG 60
           MASLSSLL +SPYS  K + S I SNKRH V+NYVSCKA  SNNGED +SKFDRR+VLFG
Sbjct: 1   MASLSSLLLLSPYSQTKTKFS-ISSNKRHVVVNYVSCKAASSNNGEDSVSKFDRREVLFG 60

Query: 61  LGGLYGVTTLSGNDIALATDWSLSPDVHKCKPANPPAGKMDCCPPKINEIIEFTPPVLPT 120
           LGGLYGV T +G+ +A      L+PDV  C  A  P G  +CCPP+I +II F PP +  
Sbjct: 61  LGGLYGVVTATGSVLAA----PLTPDVFNCDMAQTPGGIKNCCPPRIGKIIHFVPPKIQQ 120

Query: 121 PRV-RPAVHLMSPDQIADFEKGIQMMKNLPEDDPCSFKHQAKVHCAYCDNAYKEIDSTVD 180
           P V RPA HLM+  QI + E+GIQ +K L EDDP SF HQAK+HCAYCD AY ++ S  +
Sbjct: 121 PYVRRPAAHLMNQTQIEELERGIQKLKQLDEDDPHSFSHQAKIHCAYCDYAYNQLGSITE 180

Query: 181 --FQVHSNALFFPFHRAYLYFFERILCHLLEKPDFAIPFWNWDAPPGMYMPEIYTKPSSS 240
             F+VHSNALFFPFHRAYLYFFERIL H +  PDFAIPFWNWD+PPGM+MPE Y +PSSS
Sbjct: 181 TRFEVHSNALFFPFHRAYLYFFERILRHYIGNPDFAIPFWNWDSPPGMHMPEFYNRPSSS 240

Query: 241 LYDENRNECHQPPKLLNLNYGGKKLKDPNIIVDYNLRWMNDQMNVETAKEFLGQVMKRGS 300
           LYD  R+  H P KL++LNY GK       +VD NLRWMND+M V++ + FLG+V+K G 
Sbjct: 241 LYDNLRDPRHDPHKLIDLNYNGKTEDHSFDVVDCNLRWMNDRMKVQSPRSFLGRVLKAGE 300

Query: 301 CLEDGS-GSIEMSPHNNIHIWVGDPEAKITGEDMGHFHSAGKDPLFYAHHANVDRMWNIW 360
             +    G+IE SPHNNIH WVG        EDMG F+SA  DPLFYAHH N+DR+WNIW
Sbjct: 301 TPKPSDMGTIEQSPHNNIHDWVGTKTPHF--EDMGSFYSAALDPLFYAHHTNIDRLWNIW 360

Query: 361 QKMKDRPRDHTDPDWLNSSFVLYDENRKA--------------------VPIPWLYTPAI 420
           + +   P+DHTDPDWLNSSFV YDEN+ A                    VP+PWL    I
Sbjct: 361 KTLDGNPKDHTDPDWLNSSFVFYDENKNAVRIEVSDCLDTEKLGYVYQEVPLPWLLLNKI 420

Query: 421 NRPNLSASRLRNLPPTTDFPQILSSDITTVVDRPEVASPEERINKNEVLVLEDVEFDPTL 480
             PN         PP  +FPQ L+SDITT+VDRPE A   E +  + VLVLEDVE+DPT 
Sbjct: 421 PSPNPWIRTEEPEPPIINFPQTLNSDITTMVDRPEEAGSGEEV--DVVLVLEDVEYDPTT 480

Query: 481 PLHFDVLLNVGDEGEDVRAGHIEFVGTFTNVPHGRMTHHITSLHFVITKKIRQLGLEGEN 540
            +HFDVL+NV DE +D  A  +EFVG+F N+PHG   H  TSL FVIT+ I+ LGLE + 
Sbjct: 481 RVHFDVLVNVVDEAQDRCAAFLEFVGSFKNIPHGLHHHDKTSLRFVITEVIQGLGLERDK 540

Query: 541 KVAVKIVPNVNGGDKIKIGGIKIQLEDI 543
            +AVKI P V GG+ + +GGIKIQLE +
Sbjct: 541 NLAVKIEPMV-GGEGVTLGGIKIQLEPV 558

BLAST of PI0001191 vs. ExPASy TrEMBL
Match: A0A0A0LUI1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G073090 PE=3 SV=1)

HSP 1 Score: 607.4 bits (1565), Expect = 5.7e-170
Identity = 319/567 (56.26%), Postives = 386/567 (68.08%), Query Frame = 0

Query: 1   MASLSSLLSVSPYSGRKIQSSIIPSNKRHGVINYVSCK-ASNNGEDFLSKFDRRDVLFGL 60
           MASLSSLL +S YS  K +SS I SNKRH V+NYVSCK ASNNGED +SKFDRR+VLFGL
Sbjct: 1   MASLSSLLLLSSYSQTKTKSS-ISSNKRHVVVNYVSCKAASNNGEDSVSKFDRREVLFGL 60

Query: 61  GGLYGVTTLSGNDIALATDWSLSPDVHKCKPANPPAGKMDCCPPKINEIIEFTPPVLPTP 120
           GGLYGV T + + +A      L+PD+  C  A  P G  DCCPP+I +II F PP +  P
Sbjct: 61  GGLYGVVTATSSVLAA----PLTPDIFNCHMAKTPGGIKDCCPPQIGKIIHFVPPKIQQP 120

Query: 121 RV-RPAVHLMSPDQIADFEKGIQMMKNLPEDDPCSFKHQAKVHCAYCDNAYKEIDSTVD- 180
            V RPA HLM+  QI + E GI+ +K L E DP SF HQAK+HCAYCD  Y ++ S  + 
Sbjct: 121 YVRRPAAHLMNQTQIEELESGIKKLKQLDEGDPHSFLHQAKIHCAYCDYVYNQLGSITET 180

Query: 181 -FQVHSNALFFPFHRAYLYFFERILCHLLEKPDFAIPFWNWDAPPGMYMPEIYTKPSSSL 240
            F VHSNALFFPFHRAYLYFFERIL H +  P+FAIPFWNWD+PPGMYMPE Y +PSSSL
Sbjct: 181 RFGVHSNALFFPFHRAYLYFFERILRHYIGNPNFAIPFWNWDSPPGMYMPEFYNRPSSSL 240

Query: 241 YDENRNECHQPPKLLNLNYGGKKLKDPNIIVDYNLRWMNDQMNVETAKEFLGQVMKRGSC 300
           YD  R+  H P KL+ LNY GK       +VD NLRWMND+M V++ + FLG+V+K G  
Sbjct: 241 YDHLRDPRHDPHKLIYLNYNGKTEDHSFDVVDCNLRWMNDRMQVQSPRSFLGRVLKAGET 300

Query: 301 LE-DGSGSIEMSPHNNIHIWVGDPEAKITGEDMGHFHSAGKDPLFYAHHANVDRMWNIWQ 360
                 G+IE SPHNNIH W+G        EDMG F+SA  DPLFYAHHAN+DR+WNIW+
Sbjct: 301 PNPTDMGTIEQSPHNNIHDWLGTKTPHF--EDMGSFYSAALDPLFYAHHANIDRLWNIWK 360

Query: 361 KMKDRPRDHTDPDWLNSSFVLYDENRKA--------------------VPIPWLYTPAIN 420
            +   P+DH DPDWLNSSFV YDEN+ A                    VP+PWL+   I 
Sbjct: 361 TLDGNPKDHIDPDWLNSSFVFYDENKNAVNIKVSDCLDTEKLGYVYQEVPLPWLHMNKIP 420

Query: 421 RPNLSASRLRNLPPTTDFPQILSSDITTVVDRPEVASPEERINKNEVLVLEDVEFDPTLP 480
            PN       + PP  +FPQIL+SDITT+VDRPE     E +  +EVLVLEDVE+DPT  
Sbjct: 421 TPNPWIRTEESEPPIVNFPQILNSDITTMVDRPEEDRSGEEV--DEVLVLEDVEYDPTTR 480

Query: 481 LHFDVLLNVGDEGEDVRAGHIEFVGTFTNVPHGRMTHHITSLHFVITKKIRQLGLEGENK 540
           +HFDVL+NV D+ +D  A  +EFVG+F N+PHG   H  TSL FVIT+ I+ LGLE +  
Sbjct: 481 VHFDVLVNVVDKAQDRCAAFLEFVGSFKNIPHGLHHHDKTSLRFVITEVIQGLGLERDKN 540

Query: 541 VAVKIVPNVNGGDKIKIGGIKIQLEDI 543
           +AVKI P V GG+ + +GGIKIQLE +
Sbjct: 541 LAVKIEPMV-GGEGVTVGGIKIQLEPL 557

BLAST of PI0001191 vs. NCBI nr
Match: KAA0056965.1 (polyphenol oxidase I [Cucumis melo var. makuwa] >TYK26391.1 polyphenol oxidase I [Cucumis melo var. makuwa] >TYK26393.1 polyphenol oxidase I [Cucumis melo var. makuwa])

HSP 1 Score: 958.4 bits (2476), Expect = 2.7e-275
Identity = 469/565 (83.01%), Postives = 500/565 (88.50%), Query Frame = 0

Query: 1   MASLSSLLSVSPYSGRKIQSSIIPSNKRHGVINYVSCKASNNGEDFLSKFDRRDVLFGLG 60
           MASLSSLLSVSPYSG KIQ S+IP NKR GV N+VSCKASNNGED ++KFDRRD+LFGLG
Sbjct: 1   MASLSSLLSVSPYSGCKIQPSVIPLNKRRGVNNHVSCKASNNGEDSVNKFDRRDMLFGLG 60

Query: 61  GLYGVTTLSGNDIALATDWSLSPDVHKCKPANPPA--GKMDCCPPKINEIIEFTPPVLPT 120
           GLYGV TLSG DIALA DWSLSPDVH CKP  P A   KMDCCPPKI EIIEFTPPV+PT
Sbjct: 61  GLYGVATLSGKDIALADDWSLSPDVHNCKPVKPSAVGKKMDCCPPKIKEIIEFTPPVVPT 120

Query: 121 PRVRPAVHLMSPDQIADFEKGIQMMKNLPEDDPCSFKHQAKVHCAYCDNAYKEIDSTVDF 180
           PRVRPAVHLM+P+QIAD EKGIQMMK LPEDDP SF HQAKVHCAYCD+AYK++DST+DF
Sbjct: 121 PRVRPAVHLMTPEQIADLEKGIQMMKMLPEDDPRSFAHQAKVHCAYCDDAYKQMDSTMDF 180

Query: 181 QVHSNALFFPFHRAYLYFFERILCHLLEKPDFAIPFWNWDAPPGMYMPEIYTKPSSSLYD 240
           QVH NALFFPFHRAYLYFFERILCHLLEKPDFAIPFWNWDAPPGM MP+IYTKPSSSLYD
Sbjct: 181 QVHENALFFPFHRAYLYFFERILCHLLEKPDFAIPFWNWDAPPGMCMPKIYTKPSSSLYD 240

Query: 241 ENRNECHQPPKLLNLNYGGKKLKDPNIIVDYNLRWMNDQMNVETAKEFLGQVMKRGSCLE 300
           ENR++CHQPPKLLNLNYGGK++KDP  IVD+NLRWMNDQM+VETAKEFLG+V+KRG+  E
Sbjct: 241 ENRDKCHQPPKLLNLNYGGKEVKDPE-IVDFNLRWMNDQMSVETAKEFLGKVIKRGNGPE 300

Query: 301 DGSGSIEMSPHNNIHIWVGDPEAKITGEDMGHFHSAGKDPLFYAHHANVDRMWNIWQKMK 360
           +GSGSIE SPHNNIHIWVGDPEA I GEDMGHF+SAG+DPLFYAHHANVDRMWNIWQKMK
Sbjct: 301 NGSGSIETSPHNNIHIWVGDPEATIHGEDMGHFYSAGRDPLFYAHHANVDRMWNIWQKMK 360

Query: 361 DRPRDHTDPDWLNSSFVLYDENRKA--------------------VPIPWLYTPAINRPN 420
           DRPRDHTD DWLNSSFVLYDENRKA                    VPIPW  TP I RP 
Sbjct: 361 DRPRDHTDMDWLNSSFVLYDENRKAVRIKVSQCLDSKILGYVYQDVPIPWKDTPPIRRPK 420

Query: 421 LSASRLRNLPPTTDFPQILSSDITTVVDRPEVASPEERINKNEVLVLEDVEFDPTLPLHF 480
              S L NLPPT DFPQILSSDITT+VDRPEVASPEERINKNEVLVLEDVE+DPTLPLHF
Sbjct: 421 QERS-LGNLPPTIDFPQILSSDITTMVDRPEVASPEERINKNEVLVLEDVEYDPTLPLHF 480

Query: 481 DVLLNVGDEGEDVRAGHIEFVGTFTNVPHGRMTHHITSLHFVITKKIRQLGLEGENKVAV 540
           DVLLNVGDEGEDVRAGHIEFVGTFTNVPHGR T+H TSLHFVITKKI+QLGLEG+ KVAV
Sbjct: 481 DVLLNVGDEGEDVRAGHIEFVGTFTNVPHGRTTYHKTSLHFVITKKIQQLGLEGDKKVAV 540

Query: 541 KIVPNVNGGDKIKIGGIKIQLEDIE 544
           KIVPNVNGGD+IKIGGIKIQLEDIE
Sbjct: 541 KIVPNVNGGDRIKIGGIKIQLEDIE 563

BLAST of PI0001191 vs. NCBI nr
Match: KAA0056963.1 (polyphenol oxidase I [Cucumis melo var. makuwa])

HSP 1 Score: 950.3 bits (2455), Expect = 7.3e-273
Identity = 467/565 (82.65%), Postives = 497/565 (87.96%), Query Frame = 0

Query: 1   MASLSSLLSVSPYSGRKIQSSIIPSNKRHGVINYVSCKASNNGEDFLSKFDRRDVLFGLG 60
           MASLSSLLSVSPYSG KIQ S+IP NKR GV N+VSCKASNNGED ++KFDRRD+LFGLG
Sbjct: 1   MASLSSLLSVSPYSGCKIQPSVIPLNKRRGVNNHVSCKASNNGEDSVNKFDRRDMLFGLG 60

Query: 61  GLYGVTTLSGNDIALATDWSLSPDVHKCKPANPPA--GKMDCCPPKINEIIEFTPPVLPT 120
           GLYGV TLSG DIALA DWSLSPDVH CKP  P A   KMDCCPPKI EIIEFTPPV+PT
Sbjct: 61  GLYGVATLSGKDIALADDWSLSPDVHNCKPVKPSAVGKKMDCCPPKIKEIIEFTPPVVPT 120

Query: 121 PRVRPAVHLMSPDQIADFEKGIQMMKNLPEDDPCSFKHQAKVHCAYCDNAYKEIDSTVDF 180
           PRVRPAVHLM+PDQIAD EKGIQMMK LPEDDP SF HQAKVHCAYCD+AYK++DST+DF
Sbjct: 121 PRVRPAVHLMTPDQIADLEKGIQMMKMLPEDDPRSFAHQAKVHCAYCDDAYKQMDSTMDF 180

Query: 181 QVHSNALFFPFHRAYLYFFERILCHLLEKPDFAIPFWNWDAPPGMYMPEIYTKPSSSLYD 240
           QVH NALFFPFHRAYLYFFERILCHLLEKPDFAIPFWNWDAPPGM MP+IYTKPSSSLYD
Sbjct: 181 QVHENALFFPFHRAYLYFFERILCHLLEKPDFAIPFWNWDAPPGMCMPKIYTKPSSSLYD 240

Query: 241 ENRNECHQPPKLLNLNYGGKKLKDPNIIVDYNLRWMNDQMNVETAKEFLGQVMKRGSCLE 300
           ENR+ECHQPPKLLNLNYGGKK+KD   IVD+NLRWMNDQM+VETA+EFLG+V+KRG+C E
Sbjct: 241 ENRDECHQPPKLLNLNYGGKKVKDSE-IVDFNLRWMNDQMSVETAREFLGKVIKRGNCPE 300

Query: 301 DGSGSIEMSPHNNIHIWVGDPEAKITGEDMGHFHSAGKDPLFYAHHANVDRMWNIWQKMK 360
           +GSGSIE SPHNN+H WVGDPEA I GEDMGHF+SAG+DPLFYAHHANVDRMWNIWQ MK
Sbjct: 301 NGSGSIETSPHNNLHNWVGDPEATIHGEDMGHFYSAGRDPLFYAHHANVDRMWNIWQNMK 360

Query: 361 DRPRDHTDPDWLNSSFVLYDENRKA--------------------VPIPWLYTPAINRPN 420
           DR RDHTD DWLNSSFVLYDENRKA                    VPIPW  TP I RP 
Sbjct: 361 DR-RDHTDMDWLNSSFVLYDENRKAVRIKVSQCLDSKILGYVYQDVPIPWKDTPPIRRPK 420

Query: 421 LSASRLRNLPPTTDFPQILSSDITTVVDRPEVASPEERINKNEVLVLEDVEFDPTLPLHF 480
              S L NLPPT DFPQILSSDITT+VDRPEVASPEERINKNEVLVLEDVE+DPTLPLHF
Sbjct: 421 QERS-LGNLPPTIDFPQILSSDITTMVDRPEVASPEERINKNEVLVLEDVEYDPTLPLHF 480

Query: 481 DVLLNVGDEGEDVRAGHIEFVGTFTNVPHGRMTHHITSLHFVITKKIRQLGLEGENKVAV 540
           DVLLNVGDEGEDVRAGHIEFVGTFTNVPHGR T+H TSLHFVITKKI+QLGLEG+ KVAV
Sbjct: 481 DVLLNVGDEGEDVRAGHIEFVGTFTNVPHGRTTYHKTSLHFVITKKIQQLGLEGDKKVAV 540

Query: 541 KIVPNVNGGDKIKIGGIKIQLEDIE 544
           KIVPNVNGGD+IKIGGIKIQLEDIE
Sbjct: 541 KIVPNVNGGDRIKIGGIKIQLEDIE 562

BLAST of PI0001191 vs. NCBI nr
Match: KAE8652708.1 (hypothetical protein Csa_013754 [Cucumis sativus])

HSP 1 Score: 678.3 bits (1749), Expect = 5.4e-191
Identity = 358/544 (65.81%), Postives = 376/544 (69.12%), Query Frame = 0

Query: 1   MASLSSLLSVSPYSGRKIQSSIIPSNKRHGVINYVSCKASNNGEDFLSKFDRRDVLFGLG 60
           MASLSSLLSVSPYSG KIQSSIIP NKRHGV NYVSCKASN+ ED ++KFDRRDVLFGLG
Sbjct: 1   MASLSSLLSVSPYSGCKIQSSIIPLNKRHGVNNYVSCKASNHIEDSVNKFDRRDVLFGLG 60

Query: 61  GLYGVTTLSGNDIALATDWSLSPDVHKCKPANPPAG-KMDCCPPKINEIIEFTPPVLPTP 120
           GLYGV TLSGNDI LA DWSLSPDVH CKP+ P AG KMDCCPPKIN+IIEFTPPV+PT 
Sbjct: 61  GLYGVATLSGNDIGLAADWSLSPDVHNCKPSKPSAGKKMDCCPPKINKIIEFTPPVVPTL 120

Query: 121 RVRPAVHLMSPDQIADFEKGIQMMKNLPEDDPCSFKHQAKVHCAYCDNAYKEIDSTVDFQ 180
           RVRPAVHLM+PDQIAD EKGIQMMKNLPEDDPCSF HQAKVHCAYC++AYK+        
Sbjct: 121 RVRPAVHLMTPDQIADLEKGIQMMKNLPEDDPCSFAHQAKVHCAYCNDAYKQ-------- 180

Query: 181 VHSNALFFPFHRAYLYFFERILCHLLEKPDFAIPFWNWDAPPGMYMPEIYTKPSSSLYDE 240
                                                                       
Sbjct: 181 ------------------------------------------------------------ 240

Query: 241 NRNECHQPPKLLNLNYGGKKLKDPNIIVDYNLRWMNDQMNVETAKEFLGQVMKRGSCLED 300
                                                                       
Sbjct: 241 ------------------------------------------------------------ 300

Query: 301 GSGSIEMSPHNNIHIWVGDPEAKITGEDMGHFHSAGKDPLFYAHHANVDRMWNIWQKMKD 360
               IEMSPHNNIH WVGDPEA I GE+MGHFHSAGKDP+FYAHH NVDRMWNIWQKMKD
Sbjct: 301 ----IEMSPHNNIHNWVGDPEATIQGENMGHFHSAGKDPVFYAHHTNVDRMWNIWQKMKD 360

Query: 361 RPRDHTDPDWLNSSFVLYDENRKAVPIPWLYTPAINRPNLSASRLRNLPPTTDFPQILSS 420
           +PRDHTDPDWLNSSFVLYDENRKA  IPW   P I RPN    RL NLPPT DFPQILSS
Sbjct: 361 QPRDHTDPDWLNSSFVLYDENRKA--IPWKDNPPIPRPNRWERRLGNLPPTIDFPQILSS 410

Query: 421 DITTVVDRPEVASPEERINKNEVLVLEDVEFDPTLPLHFDVLLNVGDEGEDVRAGHIEFV 480
           DITT+VDRPEVASPEERINKNEVLVLEDVEFDPTLPLHFDVLLNVGD+GEDVRAG IEFV
Sbjct: 421 DITTMVDRPEVASPEERINKNEVLVLEDVEFDPTLPLHFDVLLNVGDDGEDVRAGDIEFV 410

Query: 481 GTFTNVPHGRMTHHITSLHFVITKKIRQLGLEGENKVAVKIVPNVNGGDKIKIGGIKIQL 540
           GTFTNVPHGRMTHH TSLHFV+TKKI QLGLEG+ KVAVKIVPNVNGGD+IKIGGIKIQL
Sbjct: 481 GTFTNVPHGRMTHHKTSLHFVLTKKIVQLGLEGDKKVAVKIVPNVNGGDRIKIGGIKIQL 410

Query: 541 EDIE 544
           EDIE
Sbjct: 541 EDIE 410

BLAST of PI0001191 vs. NCBI nr
Match: KAA0056962.1 (polyphenol oxidase I [Cucumis melo var. makuwa] >TYK26390.1 polyphenol oxidase I [Cucumis melo var. makuwa])

HSP 1 Score: 619.0 bits (1595), Expect = 3.9e-173
Identity = 324/568 (57.04%), Postives = 390/568 (68.66%), Query Frame = 0

Query: 1   MASLSSLLSVSPYSGRKIQSSIIPSNKRHGVINYVSCKA--SNNGEDFLSKFDRRDVLFG 60
           MASLSSLL +SPYS  K + S I SNKRH V+NYVSCKA  SNNGED +SKFDRR+VLFG
Sbjct: 1   MASLSSLLLLSPYSQTKTKFS-ISSNKRHVVVNYVSCKAASSNNGEDSVSKFDRREVLFG 60

Query: 61  LGGLYGVTTLSGNDIALATDWSLSPDVHKCKPANPPAGKMDCCPPKINEIIEFTPPVLPT 120
           LGGLYGV T +G+ +A      L+PDV  C  A  P G  +CCPP+I +II F PP +  
Sbjct: 61  LGGLYGVVTATGSVLAA----PLTPDVFNCDMAQTPGGIKNCCPPRIGKIIHFVPPKIQQ 120

Query: 121 PRV-RPAVHLMSPDQIADFEKGIQMMKNLPEDDPCSFKHQAKVHCAYCDNAYKEIDSTVD 180
           P V RPA HLM+  QI + E+GIQ +K L EDDP SF HQAK+HCAYCD AY ++ S  +
Sbjct: 121 PYVRRPAAHLMNQTQIEELERGIQKLKQLDEDDPHSFSHQAKIHCAYCDYAYNQLGSITE 180

Query: 181 --FQVHSNALFFPFHRAYLYFFERILCHLLEKPDFAIPFWNWDAPPGMYMPEIYTKPSSS 240
             F+VHSNALFFPFHRAYLYFFERIL H +  PDFAIPFWNWD+PPGM+MPE Y +PSSS
Sbjct: 181 TRFEVHSNALFFPFHRAYLYFFERILRHYIGNPDFAIPFWNWDSPPGMHMPEFYNRPSSS 240

Query: 241 LYDENRNECHQPPKLLNLNYGGKKLKDPNIIVDYNLRWMNDQMNVETAKEFLGQVMKRGS 300
           LYD  R+  H P KL++LNY GK       +VD NLRWMND+M V++ + FLG+V+K G 
Sbjct: 241 LYDNLRDPRHDPHKLIDLNYNGKTEDHSFDVVDCNLRWMNDRMKVQSPRSFLGRVLKAGE 300

Query: 301 CLEDGS-GSIEMSPHNNIHIWVGDPEAKITGEDMGHFHSAGKDPLFYAHHANVDRMWNIW 360
             +    G+IE SPHNNIH WVG        EDMG F+SA  DPLFYAHH N+DR+WNIW
Sbjct: 301 TPKPSDMGTIEQSPHNNIHDWVGTKTPHF--EDMGSFYSAALDPLFYAHHTNIDRLWNIW 360

Query: 361 QKMKDRPRDHTDPDWLNSSFVLYDENRKA--------------------VPIPWLYTPAI 420
           + +   P+DHTDPDWLNSSFV YDEN+ A                    VP+PWL    I
Sbjct: 361 KTLDGNPKDHTDPDWLNSSFVFYDENKNAVRIEVSDCLDTEKLGYVYQEVPLPWLLLNKI 420

Query: 421 NRPNLSASRLRNLPPTTDFPQILSSDITTVVDRPEVASPEERINKNEVLVLEDVEFDPTL 480
             PN         PP  +FPQ L+SDITT+VDRPE A   E +  + VLVLEDVE+DPT 
Sbjct: 421 PSPNPWIRTEEPEPPIINFPQTLNSDITTMVDRPEEAGSGEEV--DVVLVLEDVEYDPTT 480

Query: 481 PLHFDVLLNVGDEGEDVRAGHIEFVGTFTNVPHGRMTHHITSLHFVITKKIRQLGLEGEN 540
            +HFDVL+NV DE +D  A  +EFVG+F N+PHG   H  TSL FVIT+ I+ LGLE + 
Sbjct: 481 RVHFDVLVNVVDEAQDRCAAFLEFVGSFKNIPHGLHHHDKTSLRFVITEVIQGLGLERDK 540

Query: 541 KVAVKIVPNVNGGDKIKIGGIKIQLEDI 543
            +AVKI P V GG+ + +GGIKIQLE +
Sbjct: 541 NLAVKIEPMV-GGEGVTLGGIKIQLEPV 558

BLAST of PI0001191 vs. NCBI nr
Match: XP_004146641.1 (polyphenol oxidase, chloroplastic [Cucumis sativus] >KGN64649.1 hypothetical protein Csa_013698 [Cucumis sativus])

HSP 1 Score: 607.4 bits (1565), Expect = 1.2e-169
Identity = 319/567 (56.26%), Postives = 386/567 (68.08%), Query Frame = 0

Query: 1   MASLSSLLSVSPYSGRKIQSSIIPSNKRHGVINYVSCK-ASNNGEDFLSKFDRRDVLFGL 60
           MASLSSLL +S YS  K +SS I SNKRH V+NYVSCK ASNNGED +SKFDRR+VLFGL
Sbjct: 1   MASLSSLLLLSSYSQTKTKSS-ISSNKRHVVVNYVSCKAASNNGEDSVSKFDRREVLFGL 60

Query: 61  GGLYGVTTLSGNDIALATDWSLSPDVHKCKPANPPAGKMDCCPPKINEIIEFTPPVLPTP 120
           GGLYGV T + + +A      L+PD+  C  A  P G  DCCPP+I +II F PP +  P
Sbjct: 61  GGLYGVVTATSSVLAA----PLTPDIFNCHMAKTPGGIKDCCPPQIGKIIHFVPPKIQQP 120

Query: 121 RV-RPAVHLMSPDQIADFEKGIQMMKNLPEDDPCSFKHQAKVHCAYCDNAYKEIDSTVD- 180
            V RPA HLM+  QI + E GI+ +K L E DP SF HQAK+HCAYCD  Y ++ S  + 
Sbjct: 121 YVRRPAAHLMNQTQIEELESGIKKLKQLDEGDPHSFLHQAKIHCAYCDYVYNQLGSITET 180

Query: 181 -FQVHSNALFFPFHRAYLYFFERILCHLLEKPDFAIPFWNWDAPPGMYMPEIYTKPSSSL 240
            F VHSNALFFPFHRAYLYFFERIL H +  P+FAIPFWNWD+PPGMYMPE Y +PSSSL
Sbjct: 181 RFGVHSNALFFPFHRAYLYFFERILRHYIGNPNFAIPFWNWDSPPGMYMPEFYNRPSSSL 240

Query: 241 YDENRNECHQPPKLLNLNYGGKKLKDPNIIVDYNLRWMNDQMNVETAKEFLGQVMKRGSC 300
           YD  R+  H P KL+ LNY GK       +VD NLRWMND+M V++ + FLG+V+K G  
Sbjct: 241 YDHLRDPRHDPHKLIYLNYNGKTEDHSFDVVDCNLRWMNDRMQVQSPRSFLGRVLKAGET 300

Query: 301 LE-DGSGSIEMSPHNNIHIWVGDPEAKITGEDMGHFHSAGKDPLFYAHHANVDRMWNIWQ 360
                 G+IE SPHNNIH W+G        EDMG F+SA  DPLFYAHHAN+DR+WNIW+
Sbjct: 301 PNPTDMGTIEQSPHNNIHDWLGTKTPHF--EDMGSFYSAALDPLFYAHHANIDRLWNIWK 360

Query: 361 KMKDRPRDHTDPDWLNSSFVLYDENRKA--------------------VPIPWLYTPAIN 420
            +   P+DH DPDWLNSSFV YDEN+ A                    VP+PWL+   I 
Sbjct: 361 TLDGNPKDHIDPDWLNSSFVFYDENKNAVNIKVSDCLDTEKLGYVYQEVPLPWLHMNKIP 420

Query: 421 RPNLSASRLRNLPPTTDFPQILSSDITTVVDRPEVASPEERINKNEVLVLEDVEFDPTLP 480
            PN       + PP  +FPQIL+SDITT+VDRPE     E +  +EVLVLEDVE+DPT  
Sbjct: 421 TPNPWIRTEESEPPIVNFPQILNSDITTMVDRPEEDRSGEEV--DEVLVLEDVEYDPTTR 480

Query: 481 LHFDVLLNVGDEGEDVRAGHIEFVGTFTNVPHGRMTHHITSLHFVITKKIRQLGLEGENK 540
           +HFDVL+NV D+ +D  A  +EFVG+F N+PHG   H  TSL FVIT+ I+ LGLE +  
Sbjct: 481 VHFDVLVNVVDKAQDRCAAFLEFVGSFKNIPHGLHHHDKTSLRFVITEVIQGLGLERDKN 540

Query: 541 VAVKIVPNVNGGDKIKIGGIKIQLEDI 543
           +AVKI P V GG+ + +GGIKIQLE +
Sbjct: 541 LAVKIEPMV-GGEGVTVGGIKIQLEPL 557