PI0001069 (gene) Melon (PI 482460) v1

Overview


Name	PI0001069
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	(R)-mandelonitrile lyase 1-like
Location	chr05: 5161110 .. 5165920 (-)
RNA-Seq Expression	PI0001069
Synteny	PI0001069

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCCACCGATATTTTGGAGTTTTTCTTTTTTTTTTTTTTTGGCTCCAACCGACAGACTAGTTGGTTCGTTAAAAAGCAAAATCGACCACTAACTGCCCCTCATACAGAATTACCCGATTGCGGTGGTTCGATCAGCTTCGATGGTTTGGCTTAATTTACTCACCCCTAATCTCACTTTTCTAAATAGGTCTAAATTATCTTTCTTTACTGATGATTACTCACACAATATTTGATTACTCACGCAATATTCTTTATTAAATTTCCAGATATCACCTATCTTCATGCATCTCATCGAAATATCCAATCAAATTTTGTCATGTGGTAAATTCTTCTTATTTCTTTTATGCAATGTTTTAATTATTTGTATAGATATGAATGATGAGAAGAGGATGCACAAACATAAGTGCATCCCATATTGTATCTATACGTATTATTCCATTAATAATATATATTAATTTTAAATATTGGTATCAATATAAATATTCAATAATCGATATCCATGAGTATTATTTGTAATCAGTATCAGTATTATTTATAATCAATATCAGGAGGCAATTAGTAATCTATATCAATGATTAGTCAGTTACCAATATTAATTAGTATTTGTAATCAGTAACAATGCCAGTGTTGTATCAGTATTGTTAATCTATATAAATAATTAGTATGAATAATATATATCAATAATCTGCATTAATATCTATATTGATATCCATAATTAAAATGTTAGTTTTATTTTTCTTTTTTGCTTTCATTTGTTTTTTCAATATTCATGATTATTGTTTACAATTACTTTGATAACATAACATGAGTTTAATCTTAAAATCAATTGACAATAAAAGAAGTATTGAAAATTCCACATTGAAAAAACCAAGGCGACTCTCACACTCCTTATAAGATAGAAGAACTACTCCTTTCATTACCAATTAGTTTTGAAATGAAATCTCTCACTGTATCAAATATAGTATTAGAGTCCATATAACCCAAATGGATATTCGGTTCAATAAAATCAAATCAAGATCTAATCAAGAATGATGAAGTCGGTGGGCTACTACACTCTCATGGCTAAATGGGTTATGAGAGGGAACCTCATGATATTAAATAAAGATTAAATAAAGAGTAGCTTATCTATTTTTACAAGAAAAGCGAGATGTCTTGGTTTTTAAAAAATTAAATAACTTCTTTTAAACTCACAAAATTTGGTGGACAAAATTCTCTCTAATCATGTTATTTCTCTCTAAAAAAATGAGTAAAAATCCTTTCTTTTTTTGTGTTGACCAACTGTTTACATCATAGCTTGCTGTCACGGGAATTCCAAATTATTTTAAATTCAAACTCCCATCTCTCTATATATGTACATTCAGACATGCCTCTTCACATAAAGAAATTTGAAAAAACACAAGAATGGAGCATAGTCTTAGTATGGCTTCCTTTCTTCTATTCATTCTCATATCTATGCTTCACTTTCAATTGGGATTCCCCCTCTCCTCAAGCACTAACCCTAATGAAGGTAATAAAAAGCCCCACTTTTCCTCCCAACATCATTTAATAATGGTTGCATGCTTGCATTGCTCGCTTTATCTTTGGGCTTGCCTCCTTGCATTGCATCGAACTTTTGTATCTTTGCACTCTGCTCGCTTTATCTACAATATCTCTTTTGGTCTTGGGGTGAGGATGAGGATTCTAGAGATGTCAACTTAGTTAGGATATCTTATTGCATCTATTGTCCTCTCTTGTCTTATGAATATTTTTCTAAACAAATAGTTGCCTCTAATACTTTGATTGTAAATATATCCTTTTCTTATTTTTTTTCAGATTTTAGATACATGAAGTTTGTACATGATGGTAGTGATTTGCCAACAAAAGAAAAATATGACTACATCATAATAGGAGGAGGAACAGCAGGGTGTCCATTAGCTGCAACATTATCATCAAATTACTCAGTCCTACTTCTTGAAAGAGGTAGTGAACCAACCAAATATCCCTCTGTATTGAGCGAACAAAGTTTCTTAAACGTCTATACGGTTGAAGACGACGGCAAAAATCCTTTCCAACGATTTGTTTCTGAGGATGGCGTGGAAAATTTGAGAGGTCGAGTCCTTGGTGGTACTAGTATGCTTAATGGTGGGTTCTACTCAAGGGGACATCAAGAGTTTTTTGAAACTGCTGGGGTTGATTGGGACATGGAATTGGTGAAAAAGGCTTATGAATGGGTTGAAGAGAGTGTGGTGTTTGAAGCAAGTTTGAATAATGGTTGGCAATATGCTTTTAAAAAGGGTTTGTTGGAAGCTGGAGTTGGTCCTTATAATGGATTTGATTTGAACCATCGTTTAGGGACTAAAATTGGAGGGTCAATCTTTGATAGGGAAGGAAATAGACATGGATCTGTGGAGCTTTTGAATAAGGCTCAACCCAATAATCTTAAAGTTGCAGTCCGAGCCACAGTATTGAAAATCATCTTTTCTGGTTAGTGCTAAATGGAATAATGAATCTCACCTTATTTTAATTCATTGATATATCTATAAATTTTAGAGTTCAACGTTGATTTAAGATCAATAGATATATTTTGGTTTTTCACTTTTACTTTTAAGAAATTAATCTAAATGATAAAATTATTAAAAATATAGCAAAATTTCAAATTGATAGACACTGACATTGATAGAAGTCTATATCAAGTGACTGTTATATGACTATTATAGAAATTGATACACCTCTATTAGTGTCTATCAATATCACGGATAGAAATTAATAGAAGTTTATATCAGTGACTATTATAGACATGGATAAAAGTCTATCAATGACTATCAATGATAGAATTGAAAGTTTATTATATTTTGTAAATATTTTGGTTTATTTTGTTATATTTGAAAATGTTTTTTTTTTTTTTTTTAGTTTTTTATAATAACGAATCTCACTTTATTTCATTGATATATCTATAACTATTTGATTATTGATATATCCATTGATACGAAAAAGAAAAAGAAAAAACGAAATCTTAATATTGACCTTTTAATCCTTATATTGAACACACCATTACATCCGGTTGGGATTATCTTTGGTTCGGCTTTGGTTCACACCAATTCTTTAACCGAATGAATGGTTCGGTTCCGGTTTTCTATAAAAACCTAGTCGGACCGACCACTTATACCCCTACTAATTCCATAGATGGCTTCCTCCAACTTTCAATCACACTGATTTCTTTTCTTCAACTTGTGTAGGTTTATCAGCAAGTGGGGTTTTATATTCAGACTCCAAAGGAAAGTTACACACAGCATCCATCCGAAAAAAAGGAGAGATCATACTAAGTGCAGGAGCCATTGGAAGTCCTCAACTTCTCCTTCTAAGTGGGGTTGGCCCAAAATCTCATCTTTCATCCTTAAAACTACCTGTCGTTCTTCACCAACCACATGTCGGCCAATTCATGTCCGACAATCCTCGTTTCACTGCCAGTATGGTGCTTCCATATCCATTGGTTATCACAGCCGTAAAAGTTGTTGGAACCTTAGATAACAATATCCATCTTCAATCTATCACAGGTTTTTTACCATTTTCACTACCTCCATCATTTAGCCTTCTTCCTCATGGATTTGATTCCGTCAATTTGAGCTTAGCCACCATCGTTGGGAAATTCTCTGAGGTTCTTTCCGAAGGGTCCTTGAAATTGAATTCTTCAACTGATGTGAAGAAAAATCCCATTGTTAGATTTAATTACTATTCTCATCCTGATGATCTTGCTAAATGTGTTAGAGGAGTAAGAAAAATTGGAGATTTCCTTAAAACTCCAACAATTGAAAAGATTAAGATACAAGATTTTGAGGGTAAGAAAAGATTTACGTTTTTGGAACCTCCGTTACCGGGAAATTTGTCGGATGTTGGTGTGATTGAAGAGTTTTGTAAGAAAACAGTAACGACTTATTGGCATTACCATGGAGGATGCTTGGTCGGAAAAGTTGTCGACGGTAATTATAGAGTCAACGGAATTAAAAATTTGCGCGTAGTTGATGGCTCCACTTTTTCTGATTCACCTGGAACCAATCCTATGGCTACTCTCATGATGCTTGGCCGGTGAGTTTATATATATGTATGTATGTATATTTGACTATGTTTTCAATTTGTTGTATATATTTGTCGAAATAAACAATGTTCACGGTGAAATATTGTTTATAAACCTAATTTTGAAAAAAATAAATGTTTATCGAACAATATTTTTAAATTTAATTACTTAGGTAACAATTTGATCTTTTATTTTTTGTTTTTCTAAAATATTAAAATGCGTAGAGACTACTTATACCTTTAAGTTTCGTATTTTGTTACCTATTTTTTCTCCATGGTTTTAAAAATTAAGGTAAATTTTGAAAACTATGGAAAAATAGTTTTTAGACACTTATATTTGGCTTTAGAATTTAGTTAAAAAATTCTAACAACTGTACTTAATATGAAATCATTATAAGAAATATGAAGAAAATAGACTGGTTACTGAACGGAGACCTCACTTTTTATTCTTGAAATTGTTCATCAAACTACATAGGTGTAATGTTCACCAAAAAAAAAAAAAAAGTTATTGGACAAAATGTTTGAATAAACTACAATTGATATTTTCTTAAAAAAGAAATTAGTACAGCTTTTAGTTTAATATATATGAGTAGAAATTTGAATCTGCTTTGGTATCTTGTACTCAAAGATACAGATTTTAACAAAAGTTAAACTATTGTTTAGATTATTTTATTGCCCTTTAATTTTTCTATACCGACAAATTTTGAATTGTTTTGCAGATATGTTGGGCTTAATATATTGAGAGAAAGATCAAGTTAA

mRNA sequence

ATGTCCACCGATATTTTGGAGTTTTTCTTTTTTTTTTTTTTTGGCTCCAACCGACAGACTAATTTTAGATACATGAAGTTTGTACATGATGGTAGTGATTTGCCAACAAAAGAAAAATATGACTACATCATAATAGGAGGAGGAACAGCAGGGTGTCCATTAGCTGCAACATTATCATCAAATTACTCAGTCCTACTTCTTGAAAGAGGTAGTGAACCAACCAAATATCCCTCTGTATTGAGCGAACAAAGTTTCTTAAACGTCTATACGGTTGAAGACGACGGCAAAAATCCTTTCCAACGATTTGTTTCTGAGGATGGCGTGGAAAATTTGAGAGGTCGAGTCCTTGGTGGTACTAGTATGCTTAATGGTGGGTTCTACTCAAGGGGACATCAAGAGTTTTTTGAAACTGCTGGGGCTTATGAATGGGTTGAAGAGAGTGTGGTGTTTGAAGCAAGTTTGAATAATGGTTGGCAATATGCTTTTAAAAAGGGTTTGTTGGAAGCTGGAGTTGGTCCTTATAATGGATTTGATTTGAACCATCGTTTAGGGACTAAAATTGGAGGGTCAATCTTTGATAGGGAAGGAAATAGACATGGATCTGTGGAGCTTTTGAATAAGGCTCAACCCAATAATCTTAAAGTTGCAGTCCGAGCCACAGTATTGAAAATCATCTTTTCTGGTTTATCAGCAAGTGGGGTTTTATATTCAGACTCCAAAGGAAAGTTACACACAGCATCCATCCGAAAAAAAGGAGAGATCATACTAAGTGCAGGAGCCATTGGAAGTCCTCAACTTCTCCTTCTAAGTGGGGTTGGCCCAAAATCTCATCTTTCATCCTTAAAACTACCTGTCGTTCTTCACCAACCACATGTCGGCCAATTCATGTCCGACAATCCTCGTTTCACTGCCAGTATGGTGCTTCCATATCCATTGGTTATCACAGCCGTAAAAGTTGTTGGAACCTTAGATAACAATATCCATCTTCAATCTATCACAGGTTTTTTACCATTTTCACTACCTCCATCATTTAGCCTTCTTCCTCATGGATTTGATTCCGTCAATTTGAGCTTAGCCACCATCGTTGGGAAATTCTCTGAGGTTCTTTCCGAAGGGTCCTTGAAATTGAATTCTTCAACTGATGTGAAGAAAAATCCCATTGTTAGATTTAATTACTATTCTCATCCTGATGATCTTGCTAAATGTGTTAGAGGAGTAAGAAAAATTGGAGATTTCCTTAAAACTCCAACAATTGAAAAGATTAAGATACAAGATTTTGAGGGTAAGAAAAGATTTACGTTTTTGGAACCTCCGTTACCGGGAAATTTGTCGGATGTTGGTGTGATTGAAGAGTTTTGTAAGAAAACAGTAACGACTTATTGGCATTACCATGGAGGATGCTTGGTCGGAAAAGTTGTCGACGGTAATTATAGAGTCAACGGAATTAAAAATTTGCGCGTAGTTGATGGCTCCACTTTTTCTGATTCACCTGGAACCAATCCTATGGCTACTCTCATGATGCTTGGCCGATATGTTGGGCTTAATATATTGAGAGAAAGATCAAGTTAA

Coding sequence (CDS)

ATGTCCACCGATATTTTGGAGTTTTTCTTTTTTTTTTTTTTTGGCTCCAACCGACAGACTAATTTTAGATACATGAAGTTTGTACATGATGGTAGTGATTTGCCAACAAAAGAAAAATATGACTACATCATAATAGGAGGAGGAACAGCAGGGTGTCCATTAGCTGCAACATTATCATCAAATTACTCAGTCCTACTTCTTGAAAGAGGTAGTGAACCAACCAAATATCCCTCTGTATTGAGCGAACAAAGTTTCTTAAACGTCTATACGGTTGAAGACGACGGCAAAAATCCTTTCCAACGATTTGTTTCTGAGGATGGCGTGGAAAATTTGAGAGGTCGAGTCCTTGGTGGTACTAGTATGCTTAATGGTGGGTTCTACTCAAGGGGACATCAAGAGTTTTTTGAAACTGCTGGGGCTTATGAATGGGTTGAAGAGAGTGTGGTGTTTGAAGCAAGTTTGAATAATGGTTGGCAATATGCTTTTAAAAAGGGTTTGTTGGAAGCTGGAGTTGGTCCTTATAATGGATTTGATTTGAACCATCGTTTAGGGACTAAAATTGGAGGGTCAATCTTTGATAGGGAAGGAAATAGACATGGATCTGTGGAGCTTTTGAATAAGGCTCAACCCAATAATCTTAAAGTTGCAGTCCGAGCCACAGTATTGAAAATCATCTTTTCTGGTTTATCAGCAAGTGGGGTTTTATATTCAGACTCCAAAGGAAAGTTACACACAGCATCCATCCGAAAAAAAGGAGAGATCATACTAAGTGCAGGAGCCATTGGAAGTCCTCAACTTCTCCTTCTAAGTGGGGTTGGCCCAAAATCTCATCTTTCATCCTTAAAACTACCTGTCGTTCTTCACCAACCACATGTCGGCCAATTCATGTCCGACAATCCTCGTTTCACTGCCAGTATGGTGCTTCCATATCCATTGGTTATCACAGCCGTAAAAGTTGTTGGAACCTTAGATAACAATATCCATCTTCAATCTATCACAGGTTTTTTACCATTTTCACTACCTCCATCATTTAGCCTTCTTCCTCATGGATTTGATTCCGTCAATTTGAGCTTAGCCACCATCGTTGGGAAATTCTCTGAGGTTCTTTCCGAAGGGTCCTTGAAATTGAATTCTTCAACTGATGTGAAGAAAAATCCCATTGTTAGATTTAATTACTATTCTCATCCTGATGATCTTGCTAAATGTGTTAGAGGAGTAAGAAAAATTGGAGATTTCCTTAAAACTCCAACAATTGAAAAGATTAAGATACAAGATTTTGAGGGTAAGAAAAGATTTACGTTTTTGGAACCTCCGTTACCGGGAAATTTGTCGGATGTTGGTGTGATTGAAGAGTTTTGTAAGAAAACAGTAACGACTTATTGGCATTACCATGGAGGATGCTTGGTCGGAAAAGTTGTCGACGGTAATTATAGAGTCAACGGAATTAAAAATTTGCGCGTAGTTGATGGCTCCACTTTTTCTGATTCACCTGGAACCAATCCTATGGCTACTCTCATGATGCTTGGCCGATATGTTGGGCTTAATATATTGAGAGAAAGATCAAGTTAA

Protein sequence

MSTDILEFFFFFFFGSNRQTNFRYMKFVHDGSDLPTKEKYDYIIIGGGTAGCPLAATLSSNYSVLLLERGSEPTKYPSVLSEQSFLNVYTVEDDGKNPFQRFVSEDGVENLRGRVLGGTSMLNGGFYSRGHQEFFETAGAYEWVEESVVFEASLNNGWQYAFKKGLLEAGVGPYNGFDLNHRLGTKIGGSIFDREGNRHGSVELLNKAQPNNLKVAVRATVLKIIFSGLSASGVLYSDSKGKLHTASIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQPHVGQFMSDNPRFTASMVLPYPLVITAVKVVGTLDNNIHLQSITGFLPFSLPPSFSLLPHGFDSVNLSLATIVGKFSEVLSEGSLKLNSSTDVKKNPIVRFNYYSHPDDLAKCVRGVRKIGDFLKTPTIEKIKIQDFEGKKRFTFLEPPLPGNLSDVGVIEEFCKKTVTTYWHYHGGCLVGKVVDGNYRVNGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLNILRERSS

Homology

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BLAST of PI0001069 vs. ExPASy Swiss-Prot
Match: P52706 ((R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 517.3 bits (1331), Expect = 2.0e-145
Identity = 264/516 (51.16%), Postives = 354/516 (68.60%), Query Frame = 0


Query: 21  NFRYMKFVHDGSDLPTKEKYDYIIIGGGTAGCPLAATLSSNYSVLLLERGSEPTKYPSVL 80
           +F Y++F +D +DL  +  YDY+I+GGGT+GCPLAATLS  Y VL+LERGS PT YP+VL
Sbjct: 35  DFSYLRFAYDATDLELEGSYDYVIVGGGTSGCPLAATLSEKYKVLVLERGSLPTAYPNVL 94

Query: 81  SEQSFLNVYTVEDDGKNPFQRFVSEDGVENLRGRVLGGTSMLNGGFYSRGHQEFFETAG- 140
           +   F+     EDDGK P +RFVSEDG++N+RGRVLGGTSM+N G Y+R +   +  +G 
Sbjct: 95  TADGFVYNLQQEDDGKTPVERFVSEDGIDNVRGRVLGGTSMINAGVYARANTSIYSASGV 154

Query: 141 ---------AYEWVEESVVFEASLNNGWQYAFKKGLLEAGVGPYNGFDLNHRLGTKIGGS 200
                     YEWVE+++VF+ +    WQ       LEAGV P +GF L+H  GT+I GS
Sbjct: 155 DWDMDLVNKTYEWVEDTIVFKPNY-QPWQSVTGTAFLEAGVDPNHGFSLDHEAGTRITGS 214

Query: 201 IFDREGNRHGSVELLNKAQPNNLKVAVRATVLKIIFS---GLSASGVLYSDSKGKLHTAS 260
            FD +G RH + ELLNK   NNL+V V A+V KIIFS   GL+A+GV+Y DS G  H A 
Sbjct: 215 TFDNKGTRHAADELLNKGNSNNLRVGVHASVEKIIFSNAPGLTATGVIYRDSNGTPHRAF 274

Query: 261 IRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQPHVGQFMSDNPRFTASMV 320
           +R KGE+I+SAG IG+PQLLLLSGVGP+S+LSSL +PVVL  P+VGQF+ DNPR   +++
Sbjct: 275 VRSKGEVIVSAGTIGTPQLLLLSGVGPESYLSSLNIPVVLSHPYVGQFLHDNPRNFINIL 334

Query: 321 LPYPLVITAVKVVGTLDNNIHLQSITGFLPFSLPPSFSLLPH-GFDSVNLSLATIVGKFS 380
            P P+  T V V+G + N+ +  S +  LPF+ PP FS  P   +   N + A    K +
Sbjct: 335 PPNPIEPTIVTVLG-ISNDFYQCSFSS-LPFTTPP-FSFFPSTSYPLPNSTFAHFASKVA 394

Query: 381 EVLSEGSLKLNSSTDVKKNPIVRFNYYSHPDDLAKCVRGVRKIGDFLKTPTIEKIKIQDF 440
             LS GSL L SS++V+ +P V+FNYYS+P DL+ CV G++KIG+ L T  ++  K++D 
Sbjct: 395 GPLSYGSLTLKSSSNVRVSPNVKFNYYSNPTDLSHCVSGMKKIGELLSTDALKPYKVEDL 454

Query: 441 EGKKRFTFLEPPLPGNLSDVGVIEEFCKKTVTTYWHYHGGCLVGKVVDGNYRVNGIKNLR 500
            G + F  L  PLP + +D    E FC+++V +YWHYHGGCLVGKV+DG++RV GI  LR
Sbjct: 455 PGIEGFNILGIPLPKDQTDDAAFETFCRESVASYWHYHGGCLVGKVLDGDFRVTGIDALR 514

Query: 501 VVDGSTFSDSPGTNPMATLMMLGRYVGLNILRERSS 523
           VVDGSTF  +P ++P    +MLGRYVG+ IL+ERS+
Sbjct: 515 VVDGSTFPYTPASHPQGFYLMLGRYVGIKILQERSA 546

[Close]

BLAST of PI0001069 vs. ExPASy Swiss-Prot
Match: P52707 ((R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1)

HSP 1 Score: 515.0 bits (1325), Expect = 9.9e-145
Identity = 266/517 (51.45%), Postives = 351/517 (67.89%), Query Frame = 0


Query: 21  NFRYMKFVHDGSDLPTKEKYDYIIIGGGTAGCPLAATLSSNYSVLLLERGSEPTKYPSVL 80
           +F Y+ FV+D +D   +  YDYII+GGGTAGCPLAATLS+NYSVL+LERGS PT+YP++L
Sbjct: 35  DFSYLSFVYDATDPELEGSYDYIIVGGGTAGCPLAATLSANYSVLVLERGSLPTEYPNLL 94

Query: 81  SEQSFLNVYTVEDDGKNPFQRFVSEDGVENLRGRVLGGTSMLNGGFYSRGHQEFFETAG- 140
               F+     EDDGK P +RFVSEDG++N+RGRVLGGTSM+N G Y R +  FF   G 
Sbjct: 95  ISDGFVYNLQQEDDGKTPVERFVSEDGIDNVRGRVLGGTSMINAGVYVRANTSFFNQTGI 154

Query: 141 ---------AYEWVEESVVFEASLNNGWQYAFKKGLLEAGVGPYNGFDLNHRLGTKIGGS 200
                     YEWVE+++VFE   +  WQ       LEAG+ P NGF ++H  GT++ GS
Sbjct: 155 EWDMDLVNQTYEWVEDTIVFEPD-SQTWQTVIGTAYLEAGILPNNGFSVDHLAGTRLTGS 214

Query: 201 IFDREGNRHGSVELLNKAQPNNLKVAVRATVLKIIF----SGLSASGVLYSDSKGKLHTA 260
            FD  G RH S ELLNK  PNNL+VAV+A V KIIF    SG++A GV+Y+DS G  H A
Sbjct: 215 TFDNNGTRHASDELLNKGDPNNLRVAVQAAVEKIIFSSNTSGVTAIGVIYTDSNGTTHQA 274

Query: 261 SIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQPHVGQFMSDNPRFTASM 320
            +R +GE+ILSAG IGSPQLLLLSGVGP+S+L+SL + VV   P+VGQ++ DNPR   ++
Sbjct: 275 FVRGEGEVILSAGPIGSPQLLLLSGVGPESYLTSLNISVVASHPYVGQYIYDNPRNFINI 334

Query: 321 VLPYPLVITAVKVVGTLDNNIHLQSITGFLPFSLPPSFSLLP-HGFDSVNLSLATIVGKF 380
           + P P+  + V V+G + ++ +  SI+  LPF  PP FS  P   +   N + A IV K 
Sbjct: 335 LPPNPIEASTVTVLG-ITSDFYQCSISS-LPFDTPP-FSFFPTTSYPLPNQTFAHIVNKV 394

Query: 381 SEVLSEGSLKLNSSTDVKKNPIVRFNYYSHPDDLAKCVRGVRKIGDFLKTPTIEKIKIQD 440
              LS G++ LNSS+DV+  P V+FNYYS+  DL+ CV G++K+G+ L T  +E  K++D
Sbjct: 395 PGPLSHGTVTLNSSSDVRVGPNVKFNYYSNLTDLSHCVSGMKKLGEVLSTDALEPYKVED 454

Query: 441 FEGKKRFTFLEPPLPGNLSDVGVIEEFCKKTVTTYWHYHGGCLVGKVVDGNYRVNGIKNL 500
             G   F  L  PLP N +D    E FC+++V +YWHYHGGCLVGKV+D  +RV GI  L
Sbjct: 455 LPGIDGFNILGIPLPENQTDDAAFETFCRESVASYWHYHGGCLVGKVLDDGFRVTGINAL 514

Query: 501 RVVDGSTFSDSPGTNPMATLMMLGRYVGLNILRERSS 523
           RVVDGSTF  +P ++P    +MLGRY+G+ IL+ERS+
Sbjct: 515 RVVDGSTFPSTPASHPQGFYLMLGRYMGIQILQERSA 547

[Close]

BLAST of PI0001069 vs. ExPASy Swiss-Prot
Match: Q945K2 ((R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1)

HSP 1 Score: 512.3 bits (1318), Expect = 6.4e-144
Identity = 261/516 (50.58%), Postives = 353/516 (68.41%), Query Frame = 0


Query: 21  NFRYMKFVHDGSDLPTKEKYDYIIIGGGTAGCPLAATLSSNYSVLLLERGSEPTKYPSVL 80
           +F Y+ F +D +DL  +  YDY+I+GGGT+GCPLAATLS  Y VL+LERGS PT YP+VL
Sbjct: 35  DFSYLSFAYDATDLELEGSYDYVIVGGGTSGCPLAATLSEKYKVLVLERGSLPTAYPNVL 94

Query: 81  SEQSFLNVYTVEDDGKNPFQRFVSEDGVENLRGRVLGGTSMLNGGFYSRGHQEFFETAG- 140
           +   F+     EDDGK P +RFVSEDG++N+RGRVLGGTS++N G Y+R +   +  +G 
Sbjct: 95  TADGFVYNLQQEDDGKTPVERFVSEDGIDNVRGRVLGGTSIINAGVYARANTSIYSASGV 154

Query: 141 ---------AYEWVEESVVFEASLNNGWQYAFKKGLLEAGVGPYNGFDLNHRLGTKIGGS 200
                     YEWVE+++V++ + +  WQ   K   LEAGV P +GF L+H  GT+I GS
Sbjct: 155 DWDMDLVNQTYEWVEDTIVYKPN-SQSWQSVTKTAFLEAGVHPNHGFSLDHEEGTRITGS 214

Query: 201 IFDREGNRHGSVELLNKAQPNNLKVAVRATVLKIIFS---GLSASGVLYSDSKGKLHTAS 260
            FD +G RH + ELLNK   NNL+V V A+V KIIFS   GL+A+GV+Y DS G  H A 
Sbjct: 215 TFDNKGTRHAADELLNKGNSNNLRVGVHASVEKIIFSNAPGLTATGVIYRDSNGTPHQAF 274

Query: 261 IRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQPHVGQFMSDNPRFTASMV 320
           +R KGE+I+SAG IG+PQLLLLSGVGP+S+LSSL +PVVL  P+VGQF+ DNPR   +++
Sbjct: 275 VRSKGEVIVSAGTIGTPQLLLLSGVGPESYLSSLNIPVVLSHPYVGQFLHDNPRNFINIL 334

Query: 321 LPYPLVITAVKVVGTLDNNIHLQSITGFLPFSLPPSFSLLPH-GFDSVNLSLATIVGKFS 380
            P P+  T V V+G + N+ +  S +  LPF+ PP F   P   +   N + A    K +
Sbjct: 335 PPNPIEPTIVTVLG-ISNDFYQCSFSS-LPFTTPP-FGFFPSASYPLPNSTFAHFASKVA 394

Query: 381 EVLSEGSLKLNSSTDVKKNPIVRFNYYSHPDDLAKCVRGVRKIGDFLKTPTIEKIKIQDF 440
             LS GSL L SS++V+ +P V+FNYYS+  DL+ CV G++KIG+ L T  ++  K++D 
Sbjct: 395 GPLSYGSLTLKSSSNVRVSPNVKFNYYSNLTDLSHCVSGMKKIGELLSTDALKPYKVEDL 454

Query: 441 EGKKRFTFLEPPLPGNLSDVGVIEEFCKKTVTTYWHYHGGCLVGKVVDGNYRVNGIKNLR 500
            G + F  L  PLP + +D    E FC+++V +YWHYHGGCLVGKV+DG++RV GI  LR
Sbjct: 455 PGVEGFNILGIPLPKDQTDDAAFETFCRESVASYWHYHGGCLVGKVLDGDFRVTGINALR 514

Query: 501 VVDGSTFSDSPGTNPMATLMMLGRYVGLNILRERSS 523
           VVDGSTF  +P ++P    +MLGRYVG+ IL+ERS+
Sbjct: 515 VVDGSTFPYTPASHPQGFYLMLGRYVGIKILQERSA 546

[Close]

BLAST of PI0001069 vs. ExPASy Swiss-Prot
Match: Q9SSM2 ((R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 SV=1)

HSP 1 Score: 506.9 bits (1304), Expect = 2.7e-142
Identity = 254/515 (49.32%), Postives = 354/515 (68.74%), Query Frame = 0


Query: 24  YMKFVHDGSDLPTKEKYDYIIIGGGTAGCPLAATLSSNYSVLLLERGSEPTKYPSVLSEQ 83
           +M+F+ + +D  +++ YDYII+GGGTAGCPLAATLS ++ VLLLERG  P   P+V+S  
Sbjct: 38  FMRFISNATDFASEDYYDYIIVGGGTAGCPLAATLSQSFRVLLLERGGVPYNRPNVMSHD 97

Query: 84  SFLNVYTVEDDGKNPFQRFVSEDGVENLRGRVLGGTSMLNGGFYSRGHQEFFETAG---- 143
            FL   T  ++  +P Q F+SE+GV N RGRVLGG+S +N GFYSR  ++FFE +G    
Sbjct: 98  GFLTTLTDVNNFDSPAQSFISEEGVPNARGRVLGGSSAINAGFYSRADKQFFENSGLVWD 157

Query: 144 ------AYEWVEESVVFEASLNNGWQYAFKKGLLEAGVGPYNGFDLNHRLGTKIGGSIFD 203
                 +YEWVE ++VF   L   WQ A +  LLE GV P+NGF L H++GTKIGGS FD
Sbjct: 158 LSSVNQSYEWVERAIVFRPQLRT-WQTAIRDALLEVGVHPFNGFTLEHKVGTKIGGSTFD 217

Query: 204 REGNRHGSVELLNKAQPNNLKVAVRATVLKIIF--------SGLSASGVLYSDSKGKLHT 263
           R G RH S +LL  A+ +N++VAV ATV +++         S +SA GV+Y D  G+ H 
Sbjct: 218 RTGRRHSSADLLRYARSSNIRVAVYATVERVLLASSPSVSGSNVSAIGVVYRDQLGRFHH 277

Query: 264 ASIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQPHVGQFMSDNPRFTAS 323
           A IR +GE+ILSAGA+GSPQLL LSG+GP+S+LS+  +PV L QPHVG F+ DNPR   S
Sbjct: 278 ALIRDRGEVILSAGALGSPQLLFLSGIGPRSYLSTWGIPVALDQPHVGDFVYDNPRNGIS 337

Query: 324 MVLPYPLVITAVKVVGTLDNNIHLQSITGFLPFSLPPSFSLLPHGFDSVNLSLATIVGKF 383
           +V P P+  + ++VVG  ++   L++ +  +PF+ P     +      + + + TI+ K 
Sbjct: 338 IVPPVPMENSLIQVVGVTEDGAFLEAASNVIPFASPLHSVFIRAPASPLYVPVTTIMEKI 397

Query: 384 SEVLSEGSLKLNSSTDVKKNPIVRFNYYSHPDDLAKCVRGVRKIGDFLKTPTIEKIKIQD 443
              +S G L+L +STDV+ NP+VRFNY+S P DL +CV G RKIG+ L++  ++   I++
Sbjct: 398 LGPVSIGLLRL-ASTDVRINPVVRFNYFSDPQDLERCVNGTRKIGEILRSRAMQDFMIRE 457

Query: 444 FEGKKRFTFLEPPLPGNLSDVGVIEEFCKKTVTTYWHYHGGCLVGKVVDGNYRVNGIKNL 503
           + G +RF F+  PLP + S+  V+ +FC++TV+T WHYHGG +VGKVVD + +V G+ +L
Sbjct: 458 WFGNRRFRFVGAPLPVDQSNDLVMADFCRRTVSTIWHYHGGAVVGKVVDSDLKVIGVNSL 517

Query: 504 RVVDGSTFSDSPGTNPMATLMMLGRYVGLNILRER 521
           R+VDGSTF+ SPGTNP ATLMMLGRY+GL +LRER
Sbjct: 518 RLVDGSTFNISPGTNPQATLMMLGRYMGLKMLRER 550

[Close]

BLAST of PI0001069 vs. ExPASy Swiss-Prot
Match: O82784 ((R)-mandelonitrile lyase 4 OS=Prunus serotina OX=23207 GN=MDL4 PE=2 SV=1)

HSP 1 Score: 502.7 bits (1293), Expect = 5.1e-141
Identity = 262/522 (50.19%), Postives = 346/522 (66.28%), Query Frame = 0


Query: 16  SNRQTNFRYMKFVHDGSDLPTKEKYDYIIIGGGTAGCPLAATLSSNYSVLLLERGSEPTK 75
           ++ + +F Y+KFV++  DL  +  YDYII+GGGT+GCPLAATLS+NYSVL+LERG+  T+
Sbjct: 31  TSSEHDFGYLKFVYNAVDLELEGSYDYIIVGGGTSGCPLAATLSANYSVLVLERGTIATE 90

Query: 76  YPSVLSEQSFLNVYTVEDDGKNPFQRFVSEDGVENLRGRVLGGTSMLNGGFYSRGHQEFF 135
           YP+ L+   F      +DDGK P +RFVSEDG++N+R R+LGGT+++N G Y+R ++ F+
Sbjct: 91  YPNTLTVDGFAYNLQQQDDGKTPVERFVSEDGIDNVRSRILGGTTIINAGVYARANESFY 150

Query: 136 ETAG----------AYEWVEESVVFEASLNNGWQYAFKKGLLEAGVGPYNGFDLNHRLGT 195
             +G          AYEWVE+++V++ S N  WQ       LEAGV P NGF L H  GT
Sbjct: 151 NNSGVEWDLDLVNEAYEWVEDAIVYKPS-NQSWQSITGTAFLEAGVHPDNGFGLVHEEGT 210

Query: 196 KIGGSIFDREGNRHGSVELLNKAQPNNLKVAVRATVLKIIF----SGLSASGVLYSDSKG 255
           ++ GS FD  G RH S ELLNK  P+NLKVAV A V KIIF    SGL+A GV+Y+DS G
Sbjct: 211 RLTGSTFDNSGTRHASDELLNKGDPDNLKVAVEAAVQKIIFSTESSGLTAVGVVYTDSNG 270

Query: 256 KLHTASIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQPHVGQFMSDNPR 315
             H A +  KGE+ILSAG +G+PQLLLLSGVGP+S+L+SL + VV   P+VGQ+++DNPR
Sbjct: 271 TSHRALVSGKGEVILSAGTLGTPQLLLLSGVGPESYLTSLNISVVASHPYVGQYVNDNPR 330

Query: 316 FTASMVLPYPLVITAVKVVGTLDNNIHLQSITGFLPFSLPPSFSLLP-HGFDSVNLSLAT 375
              +++ P P+  + V V+G   +    Q     LPF  PP FSL P   +   N + A 
Sbjct: 331 NFINILPPNPIEPSTVTVLGITSD--FYQCSLSSLPFDTPP-FSLFPTTSYPLPNQTFAH 390

Query: 376 IVGKFSEVLSEGSLKLNSSTDVKKNPIVRFNYYSHPDDLAKCVRGVRKIGDFLKTPTIEK 435
           IV K    LS GSL L SS++V   P V+FNY S P DL  CV G++KIG FL T  ++ 
Sbjct: 391 IVSKVPGPLSAGSLTLQSSSNVSVAPNVKFNYCSDPVDLTHCVSGMKKIGVFLSTDALKP 450

Query: 436 IKIQDFEGKKRFTFLEPPLPGNLSDVGVIEEFCKKTVTTYWHYHGGCLVGKVVDGNYRVN 495
            K+ D  G   F  L  PLP N +D    E+FC+ TV +YWHYHGG +VGKV+DGN+RV 
Sbjct: 451 YKVDDLPGIDGFNILGTPLPENQTDDAAFEKFCRDTVASYWHYHGGAIVGKVIDGNFRVT 510

Query: 496 GIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLNILRERSS 523
           GI  LRVVDGSTF  +P ++P    +MLGRYVG  I++ERS+
Sbjct: 511 GINALRVVDGSTFPATPASHPQGFYLMLGRYVGTKIVQERSA 548

[Close]

BLAST of PI0001069 vs. ExPASy TrEMBL
Match: A0A1S3BS25 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493083 PE=3 SV=1)

HSP 1 Score: 919.8 bits (2376), Expect = 4.9e-264
Identity = 459/518 (88.61%), Postives = 479/518 (92.47%), Query Frame = 0


Query: 5   ILEFFFFFFFGSNRQTN--FRYMKFVHDGSDLPTKEKYDYIIIGGGTAGCPLAATLSSNY 64
           +L F   F   SN   N  FRYMKFVHD SDLPT+E+YDYIIIGGGTAGCPLAATLSSN+
Sbjct: 31  MLHFHLGFPLSSNTSPNEDFRYMKFVHDASDLPTEEEYDYIIIGGGTAGCPLAATLSSNF 90

Query: 65  SVLLLERGSEPTKYPSVLSEQSFLNVYTVEDDGKNPFQRFVSEDGVENLRGRVLGGTSML 124
           SVLLLERGSEPTKYPSVL EQSFLNVYTVEDDG+NPFQRFVSEDGVENLRGRVLGGTSML
Sbjct: 91  SVLLLERGSEPTKYPSVLKEQSFLNVYTVEDDGENPFQRFVSEDGVENLRGRVLGGTSML 150

Query: 125 NGGFYSRGHQEFFETAG----------AYEWVEESVVFEASLNNGWQYAFKKGLLEAGVG 184
           NGGFYSRGH+EFFETAG          AYEWVEE VVFEASLNNGWQYAF+ GLLEAGVG
Sbjct: 151 NGGFYSRGHREFFETAGVKWDMELVKKAYEWVEEGVVFEASLNNGWQYAFRNGLLEAGVG 210

Query: 185 PYNGFDLNHRLGTKIGGSIFDREGNRHGSVELLNKAQPNNLKVAVRATVLKIIFSGLSAS 244
           PYN FDLNHRLGTKIGGSIFD+EGNRHGSVELLNKA PNNLKV VRATV KIIFSGLSA 
Sbjct: 211 PYNEFDLNHRLGTKIGGSIFDKEGNRHGSVELLNKAHPNNLKVVVRATVEKIIFSGLSAR 270

Query: 245 GVLYSDSKGKLHTASIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQPHV 304
           GVLYSDSKGKLHTASIR+KGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLP+VLHQPHV
Sbjct: 271 GVLYSDSKGKLHTASIRRKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPIVLHQPHV 330

Query: 305 GQFMSDNPRFTASMVLPYPLVITAVKVVGTLDNNIHLQSITGFLPFSLPPSFSLLPHGFD 364
           GQ MSDNPRFT +MVLPYPLVITAVKVVGTLD+NIHLQSITGFLPFSLPPSFSLLP GFD
Sbjct: 331 GQSMSDNPRFTVNMVLPYPLVITAVKVVGTLDDNIHLQSITGFLPFSLPPSFSLLPRGFD 390

Query: 365 SVNLSLATIVGKFSEVLSEGSLKLNSSTDVKKNPIVRFNYYSHPDDLAKCVRGVRKIGDF 424
           SVNLSLAT+VGKFSEVLSEGSLKLNSSTDVKKNPIVRFNYYSHPDDLAKCVRGVRK+GDF
Sbjct: 391 SVNLSLATVVGKFSEVLSEGSLKLNSSTDVKKNPIVRFNYYSHPDDLAKCVRGVRKMGDF 450

Query: 425 LKTPTIEKIKIQDFEGKKRFTFLEPPLPGNLSDVGVIEEFCKKTVTTYWHYHGGCLVGKV 484
           LKT TIEKIKIQDFEGK+ F FLEPPLP NLSD+GV+E++CKKTVTTYWHYHGGCLVGKV
Sbjct: 451 LKTATIEKIKIQDFEGKRSFAFLEPPLPENLSDIGVVEKYCKKTVTTYWHYHGGCLVGKV 510

Query: 485 VDGNYRVNGIKNLRVVDGSTFSDSPGTNPMATLMMLGR 511
           VDGNYRV G+KNLRVVDGSTFSDSPGTNPMATLMMLGR
Sbjct: 511 VDGNYRVMGVKNLRVVDGSTFSDSPGTNPMATLMMLGR 548

[Close]

BLAST of PI0001069 vs. ExPASy TrEMBL
Match: A0A5A7VJA1 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold231G00920 PE=3 SV=1)

HSP 1 Score: 913.3 bits (2359), Expect = 4.6e-262
Identity = 450/496 (90.73%), Postives = 469/496 (94.56%), Query Frame = 0


Query: 25  MKFVHDGSDLPTKEKYDYIIIGGGTAGCPLAATLSSNYSVLLLERGSEPTKYPSVLSEQS 84
           MKFVHD SDLPT+E+YDYIIIGGGTAGCPLAATLSSN+SVLLLERGSEPTKYPSVL EQS
Sbjct: 1   MKFVHDASDLPTEEEYDYIIIGGGTAGCPLAATLSSNFSVLLLERGSEPTKYPSVLKEQS 60

Query: 85  FLNVYTVEDDGKNPFQRFVSEDGVENLRGRVLGGTSMLNGGFYSRGHQEFFETAG----- 144
           FLNVYTVEDDG+NPFQRFVSEDGVENLRGRVLGGTSMLNGGFYSRGH+EFFETAG     
Sbjct: 61  FLNVYTVEDDGENPFQRFVSEDGVENLRGRVLGGTSMLNGGFYSRGHREFFETAGVKWDM 120

Query: 145 -----AYEWVEESVVFEASLNNGWQYAFKKGLLEAGVGPYNGFDLNHRLGTKIGGSIFDR 204
                AYEWVEE VVFEASLNNGWQYAF+ GLLEAGVGPYN FDLNHRLGTKIGGSIFD+
Sbjct: 121 ELVKKAYEWVEEGVVFEASLNNGWQYAFRNGLLEAGVGPYNEFDLNHRLGTKIGGSIFDK 180

Query: 205 EGNRHGSVELLNKAQPNNLKVAVRATVLKIIFSGLSASGVLYSDSKGKLHTASIRKKGEI 264
           EGNRHGSVELLNKA PNNLKV VRATV KIIFSGLSA GVLYSDSKGKLHTASIR+KGEI
Sbjct: 181 EGNRHGSVELLNKAHPNNLKVVVRATVEKIIFSGLSARGVLYSDSKGKLHTASIRRKGEI 240

Query: 265 ILSAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQPHVGQFMSDNPRFTASMVLPYPLVI 324
           ILSAGAIGSPQLLLLSGVGPKSHLSSLKLP+VLHQPHVGQ MSDNPRFT +MVLPYPLVI
Sbjct: 241 ILSAGAIGSPQLLLLSGVGPKSHLSSLKLPIVLHQPHVGQSMSDNPRFTVNMVLPYPLVI 300

Query: 325 TAVKVVGTLDNNIHLQSITGFLPFSLPPSFSLLPHGFDSVNLSLATIVGKFSEVLSEGSL 384
           TAVKVVGTLD+NIHLQSITGFLPFSLPPSFSLLP GFDSVNLSLAT+VGKFSEVLSEGSL
Sbjct: 301 TAVKVVGTLDDNIHLQSITGFLPFSLPPSFSLLPRGFDSVNLSLATVVGKFSEVLSEGSL 360

Query: 385 KLNSSTDVKKNPIVRFNYYSHPDDLAKCVRGVRKIGDFLKTPTIEKIKIQDFEGKKRFTF 444
           KLNSSTDVKKNPIVRFNYYSHPDDLAKCVRGVRK+GDFLKT TIEKIKIQDFEGK+ F F
Sbjct: 361 KLNSSTDVKKNPIVRFNYYSHPDDLAKCVRGVRKMGDFLKTATIEKIKIQDFEGKRSFAF 420

Query: 445 LEPPLPGNLSDVGVIEEFCKKTVTTYWHYHGGCLVGKVVDGNYRVNGIKNLRVVDGSTFS 504
           LEPPLP NLSD+GV+E++CKKTVTTYWHYHGGCLVGKVVDGNYRV G+KNLRVVDGSTFS
Sbjct: 421 LEPPLPENLSDIGVVEKYCKKTVTTYWHYHGGCLVGKVVDGNYRVMGVKNLRVVDGSTFS 480

Query: 505 DSPGTNPMATLMMLGR 511
           DSPGTNPMATLMMLGR
Sbjct: 481 DSPGTNPMATLMMLGR 496

[Close]

BLAST of PI0001069 vs. ExPASy TrEMBL
Match: A0A0A0LJ83 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G055560 PE=3 SV=1)

HSP 1 Score: 837.0 bits (2161), Expect = 4.2e-239
Identity = 410/529 (77.50%), Postives = 460/529 (86.96%), Query Frame = 0


Query: 5   ILEFFFFFFFGSNRQTN--FRYMKFVHDGSDLPTKEKYDYIIIGGGTAGCPLAATLSSNY 64
           +L F F F   SN   N   +YM FV D S+L   E+YDYI+IGGGTAGCPLA TLS+N+
Sbjct: 17  MLHFQFGFPLSSNSNPNEDLKYMNFVQDASELLENEEYDYIVIGGGTAGCPLATTLSANF 76

Query: 65  SVLLLERGSEPTKYPSVLSEQSFLNVYTVEDDGKNPFQRFVSEDGVENLRGRVLGGTSML 124
           SVLLLERG+ PTKYP+VLSEQ+F NV+T EDDG+NPFQRFVSEDGVE +RGRVLGG+SML
Sbjct: 77  SVLLLERGNVPTKYPTVLSEQAFPNVFTTEDDGENPFQRFVSEDGVEIIRGRVLGGSSML 136

Query: 125 NGGFYSRGHQEFFETAG----------AYEWVEESVVFEASLNNGWQYAFKKGLLEAGVG 184
           N GFYSRGH EFF+ +G          AYEWVEESV+FEA++NNGWQYAF+K LLEAGVG
Sbjct: 137 NAGFYSRGHPEFFDISGVDWDKELVEKAYEWVEESVIFEANINNGWQYAFRKALLEAGVG 196

Query: 185 PYNGFDLNHRLGTKIGGSIFDREGNRHGSVELLNKAQPNNLKVAVRATVLKIIFSGLSAS 244
           PY+GFDLNHR+GTKIGGSIFD+EGNRHGSVELLNKAQPNNLKV ++A V KI+FS LSA+
Sbjct: 197 PYHGFDLNHRIGTKIGGSIFDKEGNRHGSVELLNKAQPNNLKVGIQAVVQKILFSDLSAT 256

Query: 245 GVLYSDSKGKLHTASIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQPHV 304
           GV YSDSKG LHTASIRK GEII+SAG IGSPQLLLLSG+GPKSHL SLK+PVVLHQP+V
Sbjct: 257 GVSYSDSKGNLHTASIRKNGEIIVSAGTIGSPQLLLLSGIGPKSHLESLKIPVVLHQPYV 316

Query: 305 GQFMSDNPRFTASMVLPYPLVITAVKVVGTLDNNIHLQSITGFLPFSLPPSFSLLPHGFD 364
           GQ MSDNPR+T  ++LPYP+  +AVK VGTL+NN+HLQSITGFLPFSLPPSFSL+P G D
Sbjct: 317 GQSMSDNPRYTVHVILPYPMATSAVKAVGTLENNVHLQSITGFLPFSLPPSFSLVPPGLD 376

Query: 365 SVNLSLATIVGKFSEVLSEGSLKLNSSTDVKKNPIVRFNYYSHPDDLAKCVRGVRKIGDF 424
           SVNLSLA++VGKFSEVLSEGSL L SSTDVK+NP V FNYYSHPDDLAKCVRGVRK+GD 
Sbjct: 377 SVNLSLASLVGKFSEVLSEGSLYLTSSTDVKENPSVSFNYYSHPDDLAKCVRGVRKMGDL 436

Query: 425 LKTPTIEKIKIQDFEGKKRFTFLEPPLPGNLSDVGVIEEFCKKTVTTYWHYHGGCLVGKV 484
           LKT  +E IKIQDFEG KRF FLEP LPGNLSDVG++EEFCKKTVTTYWHYHGGCLVGKV
Sbjct: 437 LKTEAMENIKIQDFEGNKRFAFLEPSLPGNLSDVGLVEEFCKKTVTTYWHYHGGCLVGKV 496

Query: 485 VDGNYRVNGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLNILRERS 522
           VDGNY+V GI+NLRVVDGSTF DSPGTNPMAT+MMLGRYVGL I +ERS
Sbjct: 497 VDGNYKVIGIENLRVVDGSTFVDSPGTNPMATVMMLGRYVGLKIKQERS 545

[Close]

BLAST of PI0001069 vs. ExPASy TrEMBL
Match: A0A1S3BTD3 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493053 PE=3 SV=1)

HSP 1 Score: 836.3 bits (2159), Expect = 7.2e-239
Identity = 410/531 (77.21%), Postives = 460/531 (86.63%), Query Frame = 0


Query: 1   MSTDILEFFFFFFFGSNRQTNFRYMKFVHDGSDLPTKEKYDYIIIGGGTAGCPLAATLSS 60
           MST   +F F     +N   +F+YM FV D S+LP  E+YDYI+IGGGTAGCPLA TLSS
Sbjct: 10  MSTLHFQFGFPLSSNTNSNEDFKYMNFVQDASELPENEEYDYIVIGGGTAGCPLATTLSS 69

Query: 61  NYSVLLLERGSEPTKYPSVLSEQSFLNVYTVEDDGKNPFQRFVSEDGVENLRGRVLGGTS 120
            +SVLLLERG+ PTKYP+VL E++F N +T+EDDG+NPFQRFVSEDGVE +RGRVLGG+S
Sbjct: 70  TFSVLLLERGNVPTKYPTVLREETFPNAFTIEDDGENPFQRFVSEDGVEIIRGRVLGGSS 129

Query: 121 MLNGGFYSRGHQEFFETAG----------AYEWVEESVVFEASLNNGWQYAFKKGLLEAG 180
           MLN GFYSRGHQEFF+ +G          AYEWVEESVVFEA++NNGWQ AF+K LLEAG
Sbjct: 130 MLNAGFYSRGHQEFFDISGVDWDMELVEKAYEWVEESVVFEANINNGWQNAFRKALLEAG 189

Query: 181 VGPYNGFDLNHRLGTKIGGSIFDREGNRHGSVELLNKAQPNNLKVAVRATVLKIIFSGLS 240
           VGPYNGFDLNHR+GTKIGGSIFD+EGNRHGS+ELLNKA PNNLKVA++ATV KI+FS LS
Sbjct: 190 VGPYNGFDLNHRIGTKIGGSIFDKEGNRHGSLELLNKAHPNNLKVAIQATVQKILFSDLS 249

Query: 241 ASGVLYSDSKGKLHTASIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQP 300
           A+GV YSDSKG LHTASIRK GEII+SAG IGSPQLLLLSG+GPKSHL SL+LPVVL QP
Sbjct: 250 ATGVSYSDSKGNLHTASIRKNGEIIVSAGTIGSPQLLLLSGIGPKSHLESLELPVVLDQP 309

Query: 301 HVGQFMSDNPRFTASMVLPYPLVITAVKVVGTLDNNIHLQSITGFLPFSLPPSFSLLPHG 360
           HVGQ MSDNPR+   ++LPY L  +AVK VGTL+NN+HLQSITGFLPFSLPPSFSL+P G
Sbjct: 310 HVGQSMSDNPRYNVHVILPYQLATSAVKTVGTLENNVHLQSITGFLPFSLPPSFSLVPPG 369

Query: 361 FDSVNLSLATIVGKFSEVLSEGSLKLNSSTDVKKNPIVRFNYYSHPDDLAKCVRGVRKIG 420
            DSVNLSLA+IVGKFSEVLSEGSL L SSTDVKKNP V FNYYSHPDDLAKCVRGVRK+G
Sbjct: 370 LDSVNLSLASIVGKFSEVLSEGSLYLTSSTDVKKNPSVSFNYYSHPDDLAKCVRGVRKMG 429

Query: 421 DFLKTPTIEKIKIQDFEGKKRFTFLEPPLPGNLSDVGVIEEFCKKTVTTYWHYHGGCLVG 480
           + LKT  +EKIKIQDFEG KRF F+EP LP NLSDVG +EEFCKKTVTTYWHYHGGCLVG
Sbjct: 430 ELLKTEAMEKIKIQDFEGNKRFAFVEPSLPENLSDVGSVEEFCKKTVTTYWHYHGGCLVG 489

Query: 481 KVVDGNYRVNGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLNILRERS 522
           KVVDGNY+V GI+NLRVVDGSTF DSPGTNPMAT+MMLGRYVGLNI++ERS
Sbjct: 490 KVVDGNYKVIGIENLRVVDGSTFIDSPGTNPMATVMMLGRYVGLNIIQERS 540

[Close]

BLAST of PI0001069 vs. ExPASy TrEMBL
Match: A0A5A7UQ82 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold231G001000 PE=3 SV=1)

HSP 1 Score: 817.4 bits (2110), Expect = 3.5e-233
Identity = 401/520 (77.12%), Postives = 449/520 (86.35%), Query Frame = 0


Query: 1   MSTDILEFFFFFFFGSNRQTNFRYMKFVHDGSDLPTKEKYDYIIIGGGTAGCPLAATLSS 60
           MST   +F F     +N   +F+YM FV D S+LP  E+YDYI+IGGGTAGCPLA TLSS
Sbjct: 1   MSTLHFQFGFPLSSNTNSNEDFKYMNFVQDASELPENEEYDYIVIGGGTAGCPLATTLSS 60

Query: 61  NYSVLLLERGSEPTKYPSVLSEQSFLNVYTVEDDGKNPFQRFVSEDGVENLRGRVLGGTS 120
            +SVLLLERG+ PTKYP+VL E++F N +T+EDDG+NPFQRFVSEDGVE +RGRVLGG+S
Sbjct: 61  TFSVLLLERGNVPTKYPTVLREETFPNAFTIEDDGENPFQRFVSEDGVEIIRGRVLGGSS 120

Query: 121 MLNGGFYSRGHQEFFETAG----------AYEWVEESVVFEASLNNGWQYAFKKGLLEAG 180
           MLN GFYSRGHQEFF+ +G          AYEWVEESVVFEA++NNGWQ AF+K LLEAG
Sbjct: 121 MLNAGFYSRGHQEFFDISGVDWDMELVEKAYEWVEESVVFEANINNGWQNAFRKALLEAG 180

Query: 181 VGPYNGFDLNHRLGTKIGGSIFDREGNRHGSVELLNKAQPNNLKVAVRATVLKIIFSGLS 240
           VGPYNGFDLNHR+GTKIGGSIFD+EGNRHGS+ELLNKA PNNLKVA++ATV KI+FS LS
Sbjct: 181 VGPYNGFDLNHRIGTKIGGSIFDKEGNRHGSLELLNKAHPNNLKVAIQATVQKILFSDLS 240

Query: 241 ASGVLYSDSKGKLHTASIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQP 300
           A+GV YSDSKG LHTASIRK GEII+SAG IGSPQLLLLSG+GPKSHL SL+LPVVL QP
Sbjct: 241 ATGVSYSDSKGNLHTASIRKNGEIIVSAGTIGSPQLLLLSGIGPKSHLESLELPVVLDQP 300

Query: 301 HVGQFMSDNPRFTASMVLPYPLVITAVKVVGTLDNNIHLQSITGFLPFSLPPSFSLLPHG 360
           HVGQ MSDNPR+   ++LPY L  +AVK VGTL+NN+HLQSITGFLPFSLPPSFSL+P G
Sbjct: 301 HVGQSMSDNPRYNVHVILPYQLATSAVKTVGTLENNVHLQSITGFLPFSLPPSFSLVPPG 360

Query: 361 FDSVNLSLATIVGKFSEVLSEGSLKLNSSTDVKKNPIVRFNYYSHPDDLAKCVRGVRKIG 420
            DSVNLSLA+IVGKFSEVLSEGSL L SSTDVKKNP V FNYYSHPDDLAKCVRGVRK+G
Sbjct: 361 LDSVNLSLASIVGKFSEVLSEGSLYLTSSTDVKKNPSVSFNYYSHPDDLAKCVRGVRKMG 420

Query: 421 DFLKTPTIEKIKIQDFEGKKRFTFLEPPLPGNLSDVGVIEEFCKKTVTTYWHYHGGCLVG 480
           + LKT  +EKIKIQDFEG KRF F+EP LP NLSDVG +EEFCKKTVTTYWHYHGGCLVG
Sbjct: 421 ELLKTEAMEKIKIQDFEGNKRFAFVEPSLPENLSDVGSVEEFCKKTVTTYWHYHGGCLVG 480

Query: 481 KVVDGNYRVNGIKNLRVVDGSTFSDSPGTNPMATLMMLGR 511
           KVVDGNY+V GI+NLRVVDGSTF DSPGTNPMAT+MMLGR
Sbjct: 481 KVVDGNYKVIGIENLRVVDGSTFIDSPGTNPMATVMMLGR 520

[Close]

BLAST of PI0001069 vs. NCBI nr
Match: XP_031736419.1 ((R)-mandelonitrile lyase 1-like [Cucumis sativus])

HSP 1 Score: 949.5 bits (2453), Expect = 1.2e-272
Identity = 477/530 (90.00%), Postives = 495/530 (93.40%), Query Frame = 0


Query: 5   ILEFFFFFFFGSNRQTN--FRYMKFVHDGSDLPTKEKYDYIIIGGGTAGCPLAATLSSNY 64
           +L+F       SN + N  FRYMKFVHD SDLPTKEKYDYIIIGGGTAGCPLAATLSSN+
Sbjct: 18  MLQFQLGVSLSSNTKPNEDFRYMKFVHDASDLPTKEKYDYIIIGGGTAGCPLAATLSSNF 77

Query: 65  SVLLLERGSEPTKYPSVLSEQSFLNVYTVEDDGKNPFQRFVSEDGVENLRGRVLGGTSML 124
           SVLLLERGSEPTKYPSVL EQSFLNVYTVEDDG+NPFQRFVSEDGVENLRGRVLGGTSML
Sbjct: 78  SVLLLERGSEPTKYPSVLKEQSFLNVYTVEDDGENPFQRFVSEDGVENLRGRVLGGTSML 137

Query: 125 NGGFYSRGHQEFFETAG----------AYEWVEESVVFEASLNNGWQYAFKKGLLEAGVG 184
           NGGFYSRGHQEFFETAG          AYEWVEESVVFEASLNNGWQYAF+ GLLEAGVG
Sbjct: 138 NGGFYSRGHQEFFETAGVKWDMELVKKAYEWVEESVVFEASLNNGWQYAFRNGLLEAGVG 197

Query: 185 PYNGFDLNHRLGTKIGGSIFDREGNRHGSVELLNKAQPNNLKVAVRATVLKIIFSGLSAS 244
           PYNGF+LNHRLGTKIGGSIFD+EGNRHGSVELLNKAQPNNLKV VRATV KIIFSGLSAS
Sbjct: 198 PYNGFELNHRLGTKIGGSIFDKEGNRHGSVELLNKAQPNNLKVVVRATVEKIIFSGLSAS 257

Query: 245 GVLYSDSKGKLHTASIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQPHV 304
           GVLYSDSKG+LHTASIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQPHV
Sbjct: 258 GVLYSDSKGRLHTASIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQPHV 317

Query: 305 GQFMSDNPRFTASMVLPYPLVITAVKVVGTLDNNIHLQSITGFLPFSLPPSFSLLPHGFD 364
           GQ MSDNPRFTA++VLPYPLVITAVKVVGTLD+NIHLQSITGFLPFSLPPSFSLLPH F+
Sbjct: 318 GQSMSDNPRFTANIVLPYPLVITAVKVVGTLDDNIHLQSITGFLPFSLPPSFSLLPHRFN 377

Query: 365 SVNLSLATIVGKFSEVLSEGSLKLNSSTDVKKNPIVRFNYYSHPDDLAKCVRGVRKIGDF 424
           SVNLSLATIVGKFSEV SEGSLKLNSSTDVKKNPIVRFNYYSHPDDLAKCVRGVRK GDF
Sbjct: 378 SVNLSLATIVGKFSEVFSEGSLKLNSSTDVKKNPIVRFNYYSHPDDLAKCVRGVRKFGDF 437

Query: 425 LKTPTIEKIKIQDFEGKKRFTFLEPPLPGNLSDVGVIEEFCKKTVTTYWHYHGGCLVGKV 484
           LKTPTIEKIKIQDFEGK+ F FLEPP+P NLSD GV+E+FCK+TVTTYWHYHGGCLVGKV
Sbjct: 438 LKTPTIEKIKIQDFEGKRSFAFLEPPIPENLSDDGVVEKFCKETVTTYWHYHGGCLVGKV 497

Query: 485 VDGNYRVNGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLNILRERSS 523
           VDGNYRV GIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLNILRERSS
Sbjct: 498 VDGNYRVMGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLNILRERSS 547

[Close]

BLAST of PI0001069 vs. NCBI nr
Match: XP_031736342.1 ((R)-mandelonitrile lyase 1-like [Cucumis sativus] >KAE8651659.1 hypothetical protein Csa_021315 [Cucumis sativus])

HSP 1 Score: 948.3 bits (2450), Expect = 2.7e-272
Identity = 476/530 (89.81%), Postives = 495/530 (93.40%), Query Frame = 0


Query: 5   ILEFFFFFFFGSNRQTN--FRYMKFVHDGSDLPTKEKYDYIIIGGGTAGCPLAATLSSNY 64
           +L+F       SN + N  FRYMKFVHD SDLPTKEKYDYIIIGGGTAGCPLAATL+SN+
Sbjct: 18  MLQFQLGVSLSSNTKPNEDFRYMKFVHDASDLPTKEKYDYIIIGGGTAGCPLAATLTSNF 77

Query: 65  SVLLLERGSEPTKYPSVLSEQSFLNVYTVEDDGKNPFQRFVSEDGVENLRGRVLGGTSML 124
           SVLLLERGSEPTKYPSVL EQSFLNVYTVEDDG+NPFQRFVSEDGVENLRGRVLGGTSML
Sbjct: 78  SVLLLERGSEPTKYPSVLKEQSFLNVYTVEDDGENPFQRFVSEDGVENLRGRVLGGTSML 137

Query: 125 NGGFYSRGHQEFFETAG----------AYEWVEESVVFEASLNNGWQYAFKKGLLEAGVG 184
           NGGFYSRGHQEFFETAG          AYEWVEESVVFEASLNNGWQYAF+ GLLEAGVG
Sbjct: 138 NGGFYSRGHQEFFETAGVKWDMELVKKAYEWVEESVVFEASLNNGWQYAFRNGLLEAGVG 197

Query: 185 PYNGFDLNHRLGTKIGGSIFDREGNRHGSVELLNKAQPNNLKVAVRATVLKIIFSGLSAS 244
           PYNGF+LNHRLGTKIGGSIFD+EGNRHGSVELLNKAQPNNLKV VRATV KIIFSGLSAS
Sbjct: 198 PYNGFELNHRLGTKIGGSIFDKEGNRHGSVELLNKAQPNNLKVVVRATVEKIIFSGLSAS 257

Query: 245 GVLYSDSKGKLHTASIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQPHV 304
           GVLYSDSKG+LHTASIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQPHV
Sbjct: 258 GVLYSDSKGRLHTASIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQPHV 317

Query: 305 GQFMSDNPRFTASMVLPYPLVITAVKVVGTLDNNIHLQSITGFLPFSLPPSFSLLPHGFD 364
           GQ MSDNPRFTA++VLPYPLVITAVKVVGTLD+NIHLQSITGFLPFSLPPSFSLLPH F+
Sbjct: 318 GQSMSDNPRFTANIVLPYPLVITAVKVVGTLDDNIHLQSITGFLPFSLPPSFSLLPHRFN 377

Query: 365 SVNLSLATIVGKFSEVLSEGSLKLNSSTDVKKNPIVRFNYYSHPDDLAKCVRGVRKIGDF 424
           SVNLSLATIVGKFSEV SEGSLKLNSSTDVKKNPIVRFNYYSHPDDLAKCVRGVRK GDF
Sbjct: 378 SVNLSLATIVGKFSEVFSEGSLKLNSSTDVKKNPIVRFNYYSHPDDLAKCVRGVRKFGDF 437

Query: 425 LKTPTIEKIKIQDFEGKKRFTFLEPPLPGNLSDVGVIEEFCKKTVTTYWHYHGGCLVGKV 484
           LKTPTIEKIKIQDFEGK+ F FLEPP+P NLSD GV+E+FCK+TVTTYWHYHGGCLVGKV
Sbjct: 438 LKTPTIEKIKIQDFEGKRSFAFLEPPIPENLSDDGVVEKFCKETVTTYWHYHGGCLVGKV 497

Query: 485 VDGNYRVNGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLNILRERSS 523
           VDGNYRV GIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLNILRERSS
Sbjct: 498 VDGNYRVMGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLNILRERSS 547

[Close]

BLAST of PI0001069 vs. NCBI nr
Match: XP_008451933.1 (PREDICTED: (R)-mandelonitrile lyase 1-like [Cucumis melo])

HSP 1 Score: 919.8 bits (2376), Expect = 1.0e-263
Identity = 459/518 (88.61%), Postives = 479/518 (92.47%), Query Frame = 0


Query: 5   ILEFFFFFFFGSNRQTN--FRYMKFVHDGSDLPTKEKYDYIIIGGGTAGCPLAATLSSNY 64
           +L F   F   SN   N  FRYMKFVHD SDLPT+E+YDYIIIGGGTAGCPLAATLSSN+
Sbjct: 31  MLHFHLGFPLSSNTSPNEDFRYMKFVHDASDLPTEEEYDYIIIGGGTAGCPLAATLSSNF 90

Query: 65  SVLLLERGSEPTKYPSVLSEQSFLNVYTVEDDGKNPFQRFVSEDGVENLRGRVLGGTSML 124
           SVLLLERGSEPTKYPSVL EQSFLNVYTVEDDG+NPFQRFVSEDGVENLRGRVLGGTSML
Sbjct: 91  SVLLLERGSEPTKYPSVLKEQSFLNVYTVEDDGENPFQRFVSEDGVENLRGRVLGGTSML 150

Query: 125 NGGFYSRGHQEFFETAG----------AYEWVEESVVFEASLNNGWQYAFKKGLLEAGVG 184
           NGGFYSRGH+EFFETAG          AYEWVEE VVFEASLNNGWQYAF+ GLLEAGVG
Sbjct: 151 NGGFYSRGHREFFETAGVKWDMELVKKAYEWVEEGVVFEASLNNGWQYAFRNGLLEAGVG 210

Query: 185 PYNGFDLNHRLGTKIGGSIFDREGNRHGSVELLNKAQPNNLKVAVRATVLKIIFSGLSAS 244
           PYN FDLNHRLGTKIGGSIFD+EGNRHGSVELLNKA PNNLKV VRATV KIIFSGLSA 
Sbjct: 211 PYNEFDLNHRLGTKIGGSIFDKEGNRHGSVELLNKAHPNNLKVVVRATVEKIIFSGLSAR 270

Query: 245 GVLYSDSKGKLHTASIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQPHV 304
           GVLYSDSKGKLHTASIR+KGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLP+VLHQPHV
Sbjct: 271 GVLYSDSKGKLHTASIRRKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPIVLHQPHV 330

Query: 305 GQFMSDNPRFTASMVLPYPLVITAVKVVGTLDNNIHLQSITGFLPFSLPPSFSLLPHGFD 364
           GQ MSDNPRFT +MVLPYPLVITAVKVVGTLD+NIHLQSITGFLPFSLPPSFSLLP GFD
Sbjct: 331 GQSMSDNPRFTVNMVLPYPLVITAVKVVGTLDDNIHLQSITGFLPFSLPPSFSLLPRGFD 390

Query: 365 SVNLSLATIVGKFSEVLSEGSLKLNSSTDVKKNPIVRFNYYSHPDDLAKCVRGVRKIGDF 424
           SVNLSLAT+VGKFSEVLSEGSLKLNSSTDVKKNPIVRFNYYSHPDDLAKCVRGVRK+GDF
Sbjct: 391 SVNLSLATVVGKFSEVLSEGSLKLNSSTDVKKNPIVRFNYYSHPDDLAKCVRGVRKMGDF 450

Query: 425 LKTPTIEKIKIQDFEGKKRFTFLEPPLPGNLSDVGVIEEFCKKTVTTYWHYHGGCLVGKV 484
           LKT TIEKIKIQDFEGK+ F FLEPPLP NLSD+GV+E++CKKTVTTYWHYHGGCLVGKV
Sbjct: 451 LKTATIEKIKIQDFEGKRSFAFLEPPLPENLSDIGVVEKYCKKTVTTYWHYHGGCLVGKV 510

Query: 485 VDGNYRVNGIKNLRVVDGSTFSDSPGTNPMATLMMLGR 511
           VDGNYRV G+KNLRVVDGSTFSDSPGTNPMATLMMLGR
Sbjct: 511 VDGNYRVMGVKNLRVVDGSTFSDSPGTNPMATLMMLGR 548

[Close]

BLAST of PI0001069 vs. NCBI nr
Match: KAA0067300.1 ((R)-mandelonitrile lyase 1-like [Cucumis melo var. makuwa] >TYK10911.1 (R)-mandelonitrile lyase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 913.3 bits (2359), Expect = 9.6e-262
Identity = 450/496 (90.73%), Postives = 469/496 (94.56%), Query Frame = 0


Query: 25  MKFVHDGSDLPTKEKYDYIIIGGGTAGCPLAATLSSNYSVLLLERGSEPTKYPSVLSEQS 84
           MKFVHD SDLPT+E+YDYIIIGGGTAGCPLAATLSSN+SVLLLERGSEPTKYPSVL EQS
Sbjct: 1   MKFVHDASDLPTEEEYDYIIIGGGTAGCPLAATLSSNFSVLLLERGSEPTKYPSVLKEQS 60

Query: 85  FLNVYTVEDDGKNPFQRFVSEDGVENLRGRVLGGTSMLNGGFYSRGHQEFFETAG----- 144
           FLNVYTVEDDG+NPFQRFVSEDGVENLRGRVLGGTSMLNGGFYSRGH+EFFETAG     
Sbjct: 61  FLNVYTVEDDGENPFQRFVSEDGVENLRGRVLGGTSMLNGGFYSRGHREFFETAGVKWDM 120

Query: 145 -----AYEWVEESVVFEASLNNGWQYAFKKGLLEAGVGPYNGFDLNHRLGTKIGGSIFDR 204
                AYEWVEE VVFEASLNNGWQYAF+ GLLEAGVGPYN FDLNHRLGTKIGGSIFD+
Sbjct: 121 ELVKKAYEWVEEGVVFEASLNNGWQYAFRNGLLEAGVGPYNEFDLNHRLGTKIGGSIFDK 180

Query: 205 EGNRHGSVELLNKAQPNNLKVAVRATVLKIIFSGLSASGVLYSDSKGKLHTASIRKKGEI 264
           EGNRHGSVELLNKA PNNLKV VRATV KIIFSGLSA GVLYSDSKGKLHTASIR+KGEI
Sbjct: 181 EGNRHGSVELLNKAHPNNLKVVVRATVEKIIFSGLSARGVLYSDSKGKLHTASIRRKGEI 240

Query: 265 ILSAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQPHVGQFMSDNPRFTASMVLPYPLVI 324
           ILSAGAIGSPQLLLLSGVGPKSHLSSLKLP+VLHQPHVGQ MSDNPRFT +MVLPYPLVI
Sbjct: 241 ILSAGAIGSPQLLLLSGVGPKSHLSSLKLPIVLHQPHVGQSMSDNPRFTVNMVLPYPLVI 300

Query: 325 TAVKVVGTLDNNIHLQSITGFLPFSLPPSFSLLPHGFDSVNLSLATIVGKFSEVLSEGSL 384
           TAVKVVGTLD+NIHLQSITGFLPFSLPPSFSLLP GFDSVNLSLAT+VGKFSEVLSEGSL
Sbjct: 301 TAVKVVGTLDDNIHLQSITGFLPFSLPPSFSLLPRGFDSVNLSLATVVGKFSEVLSEGSL 360

Query: 385 KLNSSTDVKKNPIVRFNYYSHPDDLAKCVRGVRKIGDFLKTPTIEKIKIQDFEGKKRFTF 444
           KLNSSTDVKKNPIVRFNYYSHPDDLAKCVRGVRK+GDFLKT TIEKIKIQDFEGK+ F F
Sbjct: 361 KLNSSTDVKKNPIVRFNYYSHPDDLAKCVRGVRKMGDFLKTATIEKIKIQDFEGKRSFAF 420

Query: 445 LEPPLPGNLSDVGVIEEFCKKTVTTYWHYHGGCLVGKVVDGNYRVNGIKNLRVVDGSTFS 504
           LEPPLP NLSD+GV+E++CKKTVTTYWHYHGGCLVGKVVDGNYRV G+KNLRVVDGSTFS
Sbjct: 421 LEPPLPENLSDIGVVEKYCKKTVTTYWHYHGGCLVGKVVDGNYRVMGVKNLRVVDGSTFS 480

Query: 505 DSPGTNPMATLMMLGR 511
           DSPGTNPMATLMMLGR
Sbjct: 481 DSPGTNPMATLMMLGR 496

[Close]

BLAST of PI0001069 vs. NCBI nr
Match: XP_038891172.1 ((R)-mandelonitrile lyase 1-like [Benincasa hispida])

HSP 1 Score: 878.6 bits (2269), Expect = 2.6e-251
Identity = 434/517 (83.95%), Postives = 473/517 (91.49%), Query Frame = 0


Query: 16  SNRQTNFRYMKFVHDGSDLPTKEKYDYIIIGGGTAGCPLAATLSSNYSVLLLERGSEPTK 75
           +N   + RYMKFVHD S+LP KE+YDYIIIGGGTAGCPLAATLSSN+SVLLLERGSEPTK
Sbjct: 32  TNPNEDVRYMKFVHDASELPKKEEYDYIIIGGGTAGCPLAATLSSNFSVLLLERGSEPTK 91

Query: 76  YPSVLSEQSFLNVYTVEDDGKNPFQRFVSEDGVENLRGRVLGGTSMLNGGFYSRGHQEFF 135
           YP+VLSEQ+F NV+T+EDDGKNPFQRFVSEDGVENLRGRVLGGTSMLNGGFYSRGH+EFF
Sbjct: 92  YPTVLSEQTFTNVFTIEDDGKNPFQRFVSEDGVENLRGRVLGGTSMLNGGFYSRGHREFF 151

Query: 136 ETAG----------AYEWVEESVVFEASLNNGWQYAFKKGLLEAGVGPYNGFDLNHRLGT 195
           ETAG          AYEWVEESVVF+ASLNNGWQYAF+KGLLEAGVGP NGFDL H +GT
Sbjct: 152 ETAGVNWDMKLVEKAYEWVEESVVFQASLNNGWQYAFRKGLLEAGVGPDNGFDLRHSVGT 211

Query: 196 KIGGSIFDREGNRHGSVELLNKAQPNNLKVAVRATVLKIIFSGLSASGVLYSDSKGKLHT 255
           KIGGSIFD+EGNRHG+VELLNKA+PNNLKVA++ATV KI+FSGLSASGV YSDSKGKLH 
Sbjct: 212 KIGGSIFDKEGNRHGAVELLNKAEPNNLKVAIQATVQKILFSGLSASGVSYSDSKGKLHR 271

Query: 256 ASIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQPHVGQFMSDNPRFTAS 315
           A +RKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLK+PVVL+Q HVGQFMSDNPR+T +
Sbjct: 272 AFVRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKVPVVLNQHHVGQFMSDNPRYTVN 331

Query: 316 MVLPYPLVITAVKVVGTLDNNIHLQSITGFLPFSLPPSFSLLPHGFDSVNLSLATIVGKF 375
           ++LP+PLV TAVKVVGTL+NNIHLQSIT +LPFS+P SFSL+PHGFDSVNLSLATI GKF
Sbjct: 332 ILLPFPLVTTAVKVVGTLENNIHLQSITSYLPFSVPLSFSLIPHGFDSVNLSLATIAGKF 391

Query: 376 SEVLSEGSLKLNSSTDVKKNPIVRFNYYSHPDDLAKCVRGVRKIGDFLKTPTIEKIKIQD 435
           SEVLS+G L L +STDVKK+P VRFNYYSHPDDLAKCVRGVRKIGDFLKT TIE IKIQD
Sbjct: 392 SEVLSDGYLHL-TSTDVKKSPTVRFNYYSHPDDLAKCVRGVRKIGDFLKTQTIENIKIQD 451

Query: 436 FEGKKRFTFLEPPLPGNLSDVGVIEEFCKKTVTTYWHYHGGCLVGKVVDGNYRVNGIKNL 495
           FEG+K F FL P LP NLSDV  +EE+CKKTVTTYWHYHGGCLVGKVVDGNYRV GIKNL
Sbjct: 452 FEGRKGFEFLGPRLPQNLSDVSSVEEYCKKTVTTYWHYHGGCLVGKVVDGNYRVIGIKNL 511

Query: 496 RVVDGSTFSDSPGTNPMATLMMLGRYVGLNILRERSS 523
           RVVDGSTFS+SPGTNPMATL+MLGRYVGLNILRERSS
Sbjct: 512 RVVDGSTFSESPGTNPMATLLMLGRYVGLNILRERSS 547

[Close]

BLAST of PI0001069 vs. TAIR 10
Match: AT1G73050.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 506.9 bits (1304), Expect = 1.9e-143
Identity = 254/515 (49.32%), Postives = 354/515 (68.74%), Query Frame = 0


Query: 24  YMKFVHDGSDLPTKEKYDYIIIGGGTAGCPLAATLSSNYSVLLLERGSEPTKYPSVLSEQ 83
           +M+F+ + +D  +++ YDYII+GGGTAGCPLAATLS ++ VLLLERG  P   P+V+S  
Sbjct: 38  FMRFISNATDFASEDYYDYIIVGGGTAGCPLAATLSQSFRVLLLERGGVPYNRPNVMSHD 97

Query: 84  SFLNVYTVEDDGKNPFQRFVSEDGVENLRGRVLGGTSMLNGGFYSRGHQEFFETAG---- 143
            FL   T  ++  +P Q F+SE+GV N RGRVLGG+S +N GFYSR  ++FFE +G    
Sbjct: 98  GFLTTLTDVNNFDSPAQSFISEEGVPNARGRVLGGSSAINAGFYSRADKQFFENSGLVWD 157

Query: 144 ------AYEWVEESVVFEASLNNGWQYAFKKGLLEAGVGPYNGFDLNHRLGTKIGGSIFD 203
                 +YEWVE ++VF   L   WQ A +  LLE GV P+NGF L H++GTKIGGS FD
Sbjct: 158 LSSVNQSYEWVERAIVFRPQLRT-WQTAIRDALLEVGVHPFNGFTLEHKVGTKIGGSTFD 217

Query: 204 REGNRHGSVELLNKAQPNNLKVAVRATVLKIIF--------SGLSASGVLYSDSKGKLHT 263
           R G RH S +LL  A+ +N++VAV ATV +++         S +SA GV+Y D  G+ H 
Sbjct: 218 RTGRRHSSADLLRYARSSNIRVAVYATVERVLLASSPSVSGSNVSAIGVVYRDQLGRFHH 277

Query: 264 ASIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQPHVGQFMSDNPRFTAS 323
           A IR +GE+ILSAGA+GSPQLL LSG+GP+S+LS+  +PV L QPHVG F+ DNPR   S
Sbjct: 278 ALIRDRGEVILSAGALGSPQLLFLSGIGPRSYLSTWGIPVALDQPHVGDFVYDNPRNGIS 337

Query: 324 MVLPYPLVITAVKVVGTLDNNIHLQSITGFLPFSLPPSFSLLPHGFDSVNLSLATIVGKF 383
           +V P P+  + ++VVG  ++   L++ +  +PF+ P     +      + + + TI+ K 
Sbjct: 338 IVPPVPMENSLIQVVGVTEDGAFLEAASNVIPFASPLHSVFIRAPASPLYVPVTTIMEKI 397

Query: 384 SEVLSEGSLKLNSSTDVKKNPIVRFNYYSHPDDLAKCVRGVRKIGDFLKTPTIEKIKIQD 443
              +S G L+L +STDV+ NP+VRFNY+S P DL +CV G RKIG+ L++  ++   I++
Sbjct: 398 LGPVSIGLLRL-ASTDVRINPVVRFNYFSDPQDLERCVNGTRKIGEILRSRAMQDFMIRE 457

Query: 444 FEGKKRFTFLEPPLPGNLSDVGVIEEFCKKTVTTYWHYHGGCLVGKVVDGNYRVNGIKNL 503
           + G +RF F+  PLP + S+  V+ +FC++TV+T WHYHGG +VGKVVD + +V G+ +L
Sbjct: 458 WFGNRRFRFVGAPLPVDQSNDLVMADFCRRTVSTIWHYHGGAVVGKVVDSDLKVIGVNSL 517

Query: 504 RVVDGSTFSDSPGTNPMATLMMLGRYVGLNILRER 521
           R+VDGSTF+ SPGTNP ATLMMLGRY+GL +LRER
Sbjct: 518 RLVDGSTFNISPGTNPQATLMMLGRYMGLKMLRER 550

[Close]

BLAST of PI0001069 vs. TAIR 10
Match: AT1G12570.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 406.8 bits (1044), Expect = 2.7e-113
Identity = 244/553 (44.12%), Postives = 318/553 (57.50%), Query Frame = 0


Query: 16  SNRQTNFRYMKFVHDGSDLPTKEKYDYIIIGGGTAGCPLAATLSSNYSVLLLERGSEPTK 75
           S++  N+ +M+   D +  PT   YDYIIIGGGTAGCPLAATLS N SVLLLERG  P  
Sbjct: 25  SDKAPNYSFMR---DATGSPTTSYYDYIIIGGGTAGCPLAATLSQNASVLLLERGDSPYN 84

Query: 76  YPSVLSEQSFLNVYTVEDDGKNPFQRFVSEDGVENLRGRVLGGTSMLNGGFYSRGHQEFF 135
            P++    +F      +    +P QRFVSEDGV N R RVLGG S LN GFY+R   ++ 
Sbjct: 85  NPNITRLSAF-GAALSDLSESSPSQRFVSEDGVINARARVLGGGSALNAGFYTRAGTKYV 144

Query: 136 ETAG--------AYEWVEESVVFEASLNNGWQYAFKKGLLEAGVGPYNGFDLNHRLGTKI 195
              G        +Y+WVE  V F+  +   WQ A + GLLEAG+ P NGF  +H  GTK 
Sbjct: 145 RNMGWDGALANESYQWVEAKVAFQPPMGR-WQTAVRDGLLEAGIVPNNGFTYDHINGTKF 204

Query: 196 GGSIFDREGNRHGSVELLNKAQPNNLKVAVRATVLKIIFSGLS-----ASGVLYSDSKGK 255
           GG+IFDR GNRH + +LL  A P  + V + ATV +I+F         A+GV+Y D  G+
Sbjct: 205 GGTIFDRNGNRHTAADLLEYADPKGITVLLHATVHRILFRTRGTTKPIANGVVYRDRTGQ 264

Query: 256 LHTASIRK--KGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQPHVGQFMSDNP 315
            H A +++    EIILSAG +GSPQLL+LSGVGP + L +  + VV+ QPHVGQ M DNP
Sbjct: 265 AHRAYLKEGALSEIILSAGTLGSPQLLMLSGVGPSAQLQAQNITVVMDQPHVGQGMYDNP 324

Query: 316 RFTASMVLPYPLVITAVKVVGTLDNNIHLQSITG-------------------FLPFSLP 375
                +  P P+ ++ ++VVG      ++++  G                   +  FS  
Sbjct: 325 MNAVFVPSPVPVEVSLIEVVGITGEGTYVEAAGGENFGGGGGGSSGSSSTRDYYAMFS-- 384

Query: 376 PSFSLLPHGFDSVNLSLAT------IVGKFSEVLSEGSLKLNSSTDVKKNPIVRFNYYSH 435
           P  +LL        LS A       ++ K    LS G L+L  + + K NPIV FNY+ H
Sbjct: 385 PRATLLESN-SMTKLSSAQPFQGGFLLEKVMGPLSTGHLEL-KTRNPKDNPIVTFNYFQH 444

Query: 436 PDDLAKCVRGVRKIGDFLKTPTIEKIKIQD--FEGKKRFTF-----LEPPLPG-NLSDVG 495
           PDDL +CVRG++ I   +++    + K  D  FE     T      L PP  G   S   
Sbjct: 445 PDDLKRCVRGIQTIERVVQSKAFSRYKYADVSFEYLLNLTASTPVNLRPPRSGPGASLPP 504

Query: 496 VIEEFCKKTVTTYWHYHGGCLVGKVVDGNYRVNGIKNLRVVDGSTFSDSPGTNPMATLMM 521
             EEFC+ TVTT WHYHGGC+VG+VVDG+Y+V GI  LRV+D ST    PGTNP AT+MM
Sbjct: 505 SAEEFCQHTVTTIWHYHGGCVVGRVVDGDYKVIGIDRLRVIDMSTVGYCPGTNPQATVMM 564

[Close]

BLAST of PI0001069 vs. TAIR 10
Match: AT5G51950.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 394.0 bits (1011), Expect = 1.8e-109
Identity = 238/563 (42.27%), Postives = 317/563 (56.31%), Query Frame = 0


Query: 11  FFFFGSNRQTNFRYMKFVHDGSDLPTKEKYDYIIIGGGTAGCPLAATLSSNYSVLLLERG 70
           + F  S       Y  F+ D +  P   ++DYIIIGGGT+GC LAATLS N SVL+LERG
Sbjct: 17  YIFHASCSPDKAGYYSFMKDATLAPMFARFDYIIIGGGTSGCALAATLSQNASVLVLERG 76

Query: 71  SEPTKYPSVLSEQSFLNVYTVEDDGKNPFQRFVSEDGVENLRGRVLGGTSMLNGGFYSRG 130
             P   P+    ++F    +     K+  Q F+SEDGV N R RVLGG S+LN GFY+R 
Sbjct: 77  GAPYDNPTATDIENFATTLS-NTSPKSWSQLFISEDGVYNTRARVLGGGSVLNAGFYTRA 136

Query: 131 HQEFF--------ETAGAYEWVEESVVFEASLNNGWQYAFKKGLLEAGVGPYNGFDLNHR 190
             E+         E   AYEWVE+ V F+  +  GWQ AFK GLLEAG  PYNGF  +H 
Sbjct: 137 GDEYVKETEWKTDEVEAAYEWVEKKVAFQPPV-LGWQTAFKDGLLEAGEFPYNGFTYDHI 196

Query: 191 LGTKIGGSIFDREGNRHGSVELLNKAQPNNLKVAVRATVLKIIFSGL-----SASGVLYS 250
            GTKIGG+IFDR G+RH + +LL  A P N+ V + A+V KI+F+        A GV++ 
Sbjct: 197 YGTKIGGTIFDRAGHRHTAADLLEYANPGNIVVYLHASVHKILFTTKGRPRPKAYGVIFQ 256

Query: 251 DSKGKLHTASIRKK--GEIILSAGAIGSPQLLLLSGVGPKSHLSSLKL-PVVLHQPHVGQ 310
           D+ G LH A + K    E+ILSAGAIGSPQLL+LSG+GP +HL++  + P+VL  P VGQ
Sbjct: 257 DANGVLHKAELEKNSMNEVILSAGAIGSPQLLMLSGIGPAAHLAAHGIKPLVLDHPMVGQ 316

Query: 311 FMSDNPRFTASMVLPYPLVITAVKVVGTLDNNIHLQSITGFL------------------ 370
            M DNP     +  P P+ ++ ++VVG      +++  +G +                  
Sbjct: 317 GMGDNPMNAIFIPSPTPVEVSLIQVVGITKFESYIEGASGVIFSYSWTRRFFDGVLNYLN 376

Query: 371 -------PFSLPPSFSL--LPHGFDSVNLSL------ATIVGKFSEVLSEGSLKLNSSTD 430
                    +  P+ S   +   F+ +N  L        I+ K +  +S G L+L  +T+
Sbjct: 377 EMQTSRTTSTTSPTLSTQSITDFFNPINPLLNATTRAGLILQKIAGPISRGHLELR-NTN 436

Query: 431 VKKNPIVRFNYYSHPDDLAKCVRGVRKIGDFLKTPTIEKIKIQD--FEGKKRFTFLEPP- 490
              NP VRFNYY  P+DL  CV G+  I   + +    K K  D    G        P  
Sbjct: 437 PDDNPSVRFNYYQEPEDLQICVEGINTIIKVINSKAFSKFKYPDATIHGLLDLMLSVPTN 496

Query: 491 -LPGNLSDVGVIEEFCKKTVTTYWHYHGGCLVGKVVDGNYRVNGIKNLRVVDGSTFSDSP 521
             P +++ +  + +FC  TV T WHYHGGC VG+VVD NYRV GI +LRV+DGSTF  SP
Sbjct: 497 LRPRHITSMFNLRQFCIDTVMTIWHYHGGCQVGRVVDKNYRVLGIDSLRVIDGSTFLKSP 556

[Close]

BLAST of PI0001069 vs. TAIR 10
Match: AT5G51950.2 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 391.3 bits (1004), Expect = 1.2e-108
Identity = 234/544 (43.01%), Postives = 311/544 (57.17%), Query Frame = 0


Query: 30  DGSDLPTKEKYDYIIIGGGTAGCPLAATLSSNYSVLLLERGSEPTKYPSVLSEQSFLNVY 89
           D +  P   ++DYIIIGGGT+GC LAATLS N SVL+LERG  P   P+    ++F    
Sbjct: 3   DATLAPMFARFDYIIIGGGTSGCALAATLSQNASVLVLERGGAPYDNPTATDIENFATTL 62

Query: 90  TVEDDGKNPFQRFVSEDGVENLRGRVLGGTSMLNGGFYSRGHQEFF--------ETAGAY 149
           +     K+  Q F+SEDGV N R RVLGG S+LN GFY+R   E+         E   AY
Sbjct: 63  S-NTSPKSWSQLFISEDGVYNTRARVLGGGSVLNAGFYTRAGDEYVKETEWKTDEVEAAY 122

Query: 150 EWVEESVVFEASLNNGWQYAFKKGLLEAGVGPYNGFDLNHRLGTKIGGSIFDREGNRHGS 209
           EWVE+ V F+  +  GWQ AFK GLLEAG  PYNGF  +H  GTKIGG+IFDR G+RH +
Sbjct: 123 EWVEKKVAFQPPV-LGWQTAFKDGLLEAGEFPYNGFTYDHIYGTKIGGTIFDRAGHRHTA 182

Query: 210 VELLNKAQPNNLKVAVRATVLKIIFSGL-----SASGVLYSDSKGKLHTASIRKK--GEI 269
            +LL  A P N+ V + A+V KI+F+        A GV++ D+ G LH A + K    E+
Sbjct: 183 ADLLEYANPGNIVVYLHASVHKILFTTKGRPRPKAYGVIFQDANGVLHKAELEKNSMNEV 242

Query: 270 ILSAGAIGSPQLLLLSGVGPKSHLSSLKL-PVVLHQPHVGQFMSDNPRFTASMVLPYPLV 329
           ILSAGAIGSPQLL+LSG+GP +HL++  + P+VL  P VGQ M DNP     +  P P+ 
Sbjct: 243 ILSAGAIGSPQLLMLSGIGPAAHLAAHGIKPLVLDHPMVGQGMGDNPMNAIFIPSPTPVE 302

Query: 330 ITAVKVVGTLDNNIHLQSITGFL-------------------------PFSLPPSFSL-- 389
           ++ ++VVG      +++  +G +                           +  P+ S   
Sbjct: 303 VSLIQVVGITKFESYIEGASGVIFSYSWTRRFFDGVLNYLNEMQTSRTTSTTSPTLSTQS 362

Query: 390 LPHGFDSVNLSL------ATIVGKFSEVLSEGSLKLNSSTDVKKNPIVRFNYYSHPDDLA 449
           +   F+ +N  L        I+ K +  +S G L+L  +T+   NP VRFNYY  P+DL 
Sbjct: 363 ITDFFNPINPLLNATTRAGLILQKIAGPISRGHLELR-NTNPDDNPSVRFNYYQEPEDLQ 422

Query: 450 KCVRGVRKIGDFLKTPTIEKIKIQD--FEGKKRFTFLEPP--LPGNLSDVGVIEEFCKKT 509
            CV G+  I   + +    K K  D    G        P    P +++ +  + +FC  T
Sbjct: 423 ICVEGINTIIKVINSKAFSKFKYPDATIHGLLDLMLSVPTNLRPRHITSMFNLRQFCIDT 482

Query: 510 VTTYWHYHGGCLVGKVVDGNYRVNGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLNI 521
           V T WHYHGGC VG+VVD NYRV GI +LRV+DGSTF  SPGTNP AT+MMLGRY+G  I
Sbjct: 483 VMTIWHYHGGCQVGRVVDKNYRVLGIDSLRVIDGSTFLKSPGTNPQATVMMLGRYMGQRI 542

[Close]

BLAST of PI0001069 vs. TAIR 10
Match: AT1G72970.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 391.0 bits (1003), Expect = 1.5e-108
Identity = 231/541 (42.70%), Postives = 307/541 (56.75%), Query Frame = 0


Query: 27  FVHDGSDLPTKEKYDYIIIGGGTAGCPLAATLSSNYSVLLLERGSEPTKYPSVLSEQSFL 86
           F  +G D      YDYI+IGGGTAGCPLAATLS N+SVL+LERG  P    +V   ++F 
Sbjct: 54  FSSNGQD----SSYDYIVIGGGTAGCPLAATLSQNFSVLVLERGGVPFTNANVSFLRNF- 113

Query: 87  NVYTVEDDGKNPFQRFVSEDGVENLRGRVLGGTSMLNGGFYSRGHQEFFETAG------- 146
           ++   +    +  Q FVS DGV N R RVLGG S +N GFYSR    F + AG       
Sbjct: 114 HIGLADISASSASQAFVSTDGVYNARARVLGGGSCINAGFYSRADAAFVKRAGWDPKLVK 173

Query: 147 -AYEWVEESVVFEASLNNGWQYAFKKGLLEAGVGPYNGFDLNHRLGTKIGGSIFDREGNR 206
            +Y WVE  +V +  L   WQ A +  LLE GV P+NGF  +H  GTKIGG+IFDR G R
Sbjct: 174 ESYPWVEREIVHQPKLTL-WQKALRDSLLEVGVRPFNGFTYDHVSGTKIGGTIFDRFGRR 233

Query: 207 HGSVELLNKAQPNNLKVAVRATVLKIIF--SGL--SASGVLYSDSKGKLHTA--SIRKKG 266
           H + ELL  A P  L+V + ATV KI+F  SG     +GV++ D KG  H A  S RK  
Sbjct: 234 HTAAELLAYANPQKLRVLIYATVQKIVFDTSGTRPRVTGVIFKDEKGNQHQALLSNRKGS 293

Query: 267 EIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQPHVGQFMSDNPRFTASMVLPYPL 326
           E+ILS+GAIGSPQ+L+LSG+GPK  L  LK+PVVL   HVG+ M+DNP  T  +    P+
Sbjct: 294 EVILSSGAIGSPQMLMLSGIGPKKELQRLKIPVVLENEHVGKGMADNPMNTILVPSKAPI 353

Query: 327 VITAVKVVGTLDNNIHLQSITGF--LPFSLPPSFSLLPHGFDSVNLSLAT---------- 386
             + ++ VG     +++++ TGF   P S+   + ++ +  +  +   A           
Sbjct: 354 EQSLIQTVGITKMGVYVEASTGFGQSPESIHTHYGIMSNKNELFSTIPAKQRRPEATQAY 413

Query: 387 ----------------IVGKFSEVLSEGSLKLNSSTDVKKNPIVRFNYYSHPDDLAKCVR 446
                           I+ K +  +S G L L  +T+V  NP V FNY+ HP DL +CV 
Sbjct: 414 ITRNKYQLHEAFNGSFILEKLAYPISRGHLSL-VNTNVDDNPSVTFNYFKHPVDLQRCVE 473

Query: 447 GVRKIGDFLKTPTIEKIKIQDFEGKKRFTFLEPPL-----PGNLSDVGVIEEFCKKTVTT 506
            +R +   + +         D +   +   L         P  L+D   + +FCK TV T
Sbjct: 474 AIRLVSKVVTSNRFLNYTQCDKQNVHKMLSLSVKANINLRPKQLNDTKSMAQFCKDTVVT 533

Query: 507 YWHYHGGCLVGKVVDGNYRVNGIKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLNILRE 521
            WHYHGGCLVGKVV  N +V G+  LRV+DGSTF +SPGTNP AT+MM+GRY+G+ ILRE
Sbjct: 534 IWHYHGGCLVGKVVSPNRKVLGVDRLRVIDGSTFDESPGTNPQATMMMMGRYMGVKILRE 587

The following BLAST results are available for this feature:

BLAST of PI0001069 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P52706	2.0e-145	51.16	(R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1	[more]
P52707	9.9e-145	51.45	(R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1	[more]
Q945K2	6.4e-144	50.58	(R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1	[more]
Q9SSM2	2.7e-142	49.32	(R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 ...	[more]
O82784	5.1e-141	50.19	(R)-mandelonitrile lyase 4 OS=Prunus serotina OX=23207 GN=MDL4 PE=2 SV=1	[more]

BLAST of PI0001069 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BS25	4.9e-264	88.61	(R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493083 PE=3 SV=...	[more]
A0A5A7VJA1	4.6e-262	90.73	(R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]
A0A0A0LJ83	4.2e-239	77.50	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G055560 PE=3 SV=1	[more]
A0A1S3BTD3	7.2e-239	77.21	(R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493053 PE=3 SV=...	[more]
A0A5A7UQ82	3.5e-233	77.12	(R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]

BLAST of PI0001069 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031736419.1	1.2e-272	90.00	(R)-mandelonitrile lyase 1-like [Cucumis sativus]	[more]
XP_031736342.1	2.7e-272	89.81	(R)-mandelonitrile lyase 1-like [Cucumis sativus] >KAE8651659.1 hypothetical pro...	[more]
XP_008451933.1	1.0e-263	88.61	PREDICTED: (R)-mandelonitrile lyase 1-like [Cucumis melo]	[more]
KAA0067300.1	9.6e-262	90.73	(R)-mandelonitrile lyase 1-like [Cucumis melo var. makuwa] >TYK10911.1 (R)-mande...	[more]
XP_038891172.1	2.6e-251	83.95	(R)-mandelonitrile lyase 1-like [Benincasa hispida]	[more]

BLAST of PI0001069 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G73050.1	1.9e-143	49.32	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT1G12570.1	2.7e-113	44.12	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT5G51950.1	1.8e-109	42.27	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT5G51950.2	1.2e-108	43.01	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT1G72970.1	1.5e-108	42.70	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000172	Glucose-methanol-choline oxidoreductase, N-terminal	PFAM	PF00732	GMC_oxred_N	coord: 40..136 e-value: 2.6E-9 score: 36.9 coord: 160..299 e-value: 7.6E-20 score: 71.5
IPR000172	Glucose-methanol-choline oxidoreductase, N-terminal	PROSITE	PS00623	GMC_OXRED_1	coord: 113..136
IPR000172	Glucose-methanol-choline oxidoreductase, N-terminal	PROSITE	PS00624	GMC_OXRED_2	coord: 259..273
None	No IPR available	GENE3D	3.30.410.40		coord: 150..459 e-value: 3.0E-156 score: 523.1
None	No IPR available	PIRSR	PIRSR000137-2	PIRSR000137-2	coord: 358..520 e-value: 2.3E-38 score: 130.1
None	No IPR available	PANTHER	PTHR45968:SF23	(R)-MANDELONITRILE LYASE 4-RELATED	coord: 15..521
None	No IPR available	PANTHER	PTHR45968	OSJNBA0019K04.7 PROTEIN	coord: 15..521
None	No IPR available	SUPERFAMILY	54373	FAD-linked reductases, C-terminal domain	coord: 351..466
IPR012132	Glucose-methanol-choline oxidoreductase	PIRSF	PIRSF000137	Alcohol_oxidase	coord: 26..149 e-value: 1.3E-23 score: 81.5 coord: 336..522 e-value: 1.2E-30 score: 104.8 coord: 141..326 e-value: 7.2E-30 score: 102.2
IPR036188	FAD/NAD(P)-binding domain superfamily	GENE3D	3.50.50.60		coord: 40..509 e-value: 3.0E-156 score: 523.1
IPR036188	FAD/NAD(P)-binding domain superfamily	SUPERFAMILY	51905	FAD/NAD(P)-binding domain	coord: 22..520
IPR007867	Glucose-methanol-choline oxidoreductase, C-terminal	PFAM	PF05199	GMC_oxred_C	coord: 370..510 e-value: 4.5E-28 score: 98.5

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0001069.1	PI0001069.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0007165	signal transduction
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0043531	ADP binding
molecular_function	GO:0050660	flavin adenine dinucleotide binding
molecular_function	GO:0016829	lyase activity
molecular_function	GO:0003953	NAD+ nucleosidase activity
molecular_function	GO:0016614	oxidoreductase activity, acting on CH-OH group of donors