PI0000866 (gene) Melon (PI 482460) v1

Overview


Name	PI0000866
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	Reverse transcriptase
Location	chr12: 15984009 .. 15985589 (-)
RNA-Seq Expression	PI0000866
Synteny	PI0000866

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACAAAGATAGTGCACAAAGTTTGTCGGATTTCTATGGAAAAGGCACCATATGTAAGGGGCCGCACGTGGCGAGAGAATGTCCGAATCGGACTGCCTTCTACGCCTTTCAGGCGGCCTTAGCCTCAGACTCGGATGACAAGAATGGTCGAGCAGAGGATGAAGATGTCCAAATAGAAGGCGATTACCCCAGGGTAGGGGCCCTAAGGTTTCTATCGTCTCTCCAAAGGAAAGTGGAGGAGACAAGTGAACCAATAGGAAGGTGCTTAATGTATGTCGACACCCGGATCAACCAGAAGTTGGCTAAGAGTACTATGGTTGACTCCGGTGTGACCCACAACTTCATTACCGAGTCGGAAGCCTGTCGGTTAAACCTCCGCTGGGAGAAAGACACGAGAAAGGTGAAGGCCGTAAATTCTGTTGCCTTACCTATTGTCGGTGTAGTAAAAAGAACGATGATAAGTTTGGGAGAATGGAATGACCGTATAGACTTCGTGGTTGTCAAGATGGATGACTTCGACGTGGTATTGGGAATGGAATTTTTGCTAGAACATCAAGCGATACCGATGCCTCTAGCCAAATGCCTAGTGATCACGGGATCCAACCCCACCATTGTGCAGACAGACATTCGTCAGCCCAATGGATTAAAAATGATTTCGGCCCTACAGCTGAAGAAGGGCCTCGCTCGCGATGAACCGACCTTCATGGCCGTTCCGCTTGAGCAACGGGGGACTCAGAAAGAAGTAGCCCCGAAAGACATTCTGCGTGCCTTAGAGAGGCATAACGACGTGATACCTGATAGTTTGCCCAAGACTCTACTGTCGAAGAGAACAATGGGTCATGAGATCGAACTGATGCCAGGGGCAAAACCGCCTGCAAGGAATGCTTGTCATATGGCACCACCCGAGCCGGTAGAACTTCGAAAACAGTTAGAGGAGTTGTTGAGTGCATGATTCATTAGACCTGCGAAAGCTCCATATGGGGCCCCGGTCCCTTTCCAAAAGAATAGTCCACAAGCTCACAACTCCACAAGAGAATGGAAACGGACAACAGACATCGCTCGAGCTTACTTGGAGAAGGCCTCCAGACGGATGAAGAAGCAGGCAAGTAAGAAACGCCGTCCCCTCGAGTTTTGGGCGGGAAACCGAGTCCTCATCAAACTGCGACCAGAACAAGTCCGGTTTCGATGACGTAAAGACCAACACCTCGTTAGGAAATACAAGGGACCTGTTGAAGTCCTTAAGAAGGTGGAAAACACATCCTACCGAGTGGCGTTACCCACGTGGATGAAAATGCATCCAATAATTCACGTGAGTAACCTAAAGCCCTACCATCAAGACCCCGATGACACGCAACACAACGTTGTCATTTGACCAAACATCAGTCTGAACCAAAAGGAAGAAGACAGAGATGTTGAAGAAATCCTTACCGACTGGGTAAGGAAGGGCAGAAGACCCACGAGGAGAATCCACGAATACCTGATCAAGTGGAAGAATCTCCCCGTGGAAGAGACCAGTTGGGAACACGTCGAAGACCTAGAAGCTTGGAAGCAAAAGATCGAAGAATTTCAGCTTCGCTAG

mRNA sequence

ATGGACAAAGATAGTGCACAAAGTTTGTCGGATTTCTATGGAAAAGGCACCATATGTAAGGGGCCGCACGTGGCGAGAGAATGTCCGAATCGGACTGCCTTCTACGCCTTTCAGGCGGCCTTAGCCTCAGACTCGGATGACAAGAATGGTCGAGCAGAGGATGAAGATGTCCAAATAGAAGGCGATTACCCCAGGGTAGGGGCCCTAAGTCTGAACCAAAAGGAAGAAGACAGAGATGTTGAAGAAATCCTTACCGACTGGGTAAGGAAGGGCAGAAGACCCACGAGGAGAATCCACGAATACCTGATCAAGTGGAAGAATCTCCCCGTGGAAGAGACCAGTTGGGAACACGTCGAAGACCTAGAAGCTTGGAAGCAAAAGATCGAAGAATTTCAGCTTCGCTAG

Coding sequence (CDS)

ATGGACAAAGATAGTGCACAAAGTTTGTCGGATTTCTATGGAAAAGGCACCATATGTAAGGGGCCGCACGTGGCGAGAGAATGTCCGAATCGGACTGCCTTCTACGCCTTTCAGGCGGCCTTAGCCTCAGACTCGGATGACAAGAATGGTCGAGCAGAGGATGAAGATGTCCAAATAGAAGGCGATTACCCCAGGGTAGGGGCCCTAAGTCTGAACCAAAAGGAAGAAGACAGAGATGTTGAAGAAATCCTTACCGACTGGGTAAGGAAGGGCAGAAGACCCACGAGGAGAATCCACGAATACCTGATCAAGTGGAAGAATCTCCCCGTGGAAGAGACCAGTTGGGAACACGTCGAAGACCTAGAAGCTTGGAAGCAAAAGATCGAAGAATTTCAGCTTCGCTAG

Protein sequence

MDKDSAQSLSDFYGKGTICKGPHVARECPNRTAFYAFQAALASDSDDKNGRAEDEDVQIEGDYPRVGALSLNQKEEDRDVEEILTDWVRKGRRPTRRIHEYLIKWKNLPVEETSWEHVEDLEAWKQKIEEFQLR

Homology

[Close]

BLAST of PI0000866 vs. ExPASy TrEMBL
Match: A0A5A7URR9 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G001520 PE=4 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 4.1e-21
Identity = 53/66 (80.30%), Postives = 61/66 (92.42%), Query Frame = 0


Query: 69  LSLNQKEEDRDVEEILTDWVRKGRRPTRRIHEYLIKWKNLPVEETSWEHVEDLEAWKQKI 128
           + L+QK ED+DVEEIL + VR+GRRPTRRIHEYL+KWKNLPVEETSWE VEDLEAWKQKI
Sbjct: 469 IDLSQK-EDKDVEEILAERVRRGRRPTRRIHEYLVKWKNLPVEETSWERVEDLEAWKQKI 528

Query: 129 EEFQLR 135
           E+F+LR
Sbjct: 529 EDFKLR 533

[Close]

BLAST of PI0000866 vs. ExPASy TrEMBL
Match: A0A5D3BFH0 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G001520 PE=4 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 4.1e-21
Identity = 53/66 (80.30%), Postives = 61/66 (92.42%), Query Frame = 0


Query: 69  LSLNQKEEDRDVEEILTDWVRKGRRPTRRIHEYLIKWKNLPVEETSWEHVEDLEAWKQKI 128
           + L+QK ED+DVEEIL + VR+GRRPTRRIHEYL+KWKNLPVEETSWE VEDLEAWKQKI
Sbjct: 187 IDLSQK-EDKDVEEILAERVRRGRRPTRRIHEYLVKWKNLPVEETSWERVEDLEAWKQKI 246

Query: 129 EEFQLR 135
           E+F+LR
Sbjct: 247 EDFKLR 251

[Close]

BLAST of PI0000866 vs. ExPASy TrEMBL
Match: A0A5D3C8Z6 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold265G00760 PE=4 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 9.1e-21
Identity = 53/66 (80.30%), Postives = 60/66 (90.91%), Query Frame = 0


Query: 69   LSLNQKEEDRDVEEILTDWVRKGRRPTRRIHEYLIKWKNLPVEETSWEHVEDLEAWKQKI 128
            + L+QK ED+DVEEIL + VR+GRRPTRRIHEYLIKWKNLPVEETSWE VEDLE WKQKI
Sbjct: 1493 IDLSQK-EDKDVEEILAERVRRGRRPTRRIHEYLIKWKNLPVEETSWERVEDLEVWKQKI 1552

Query: 129  EEFQLR 135
            E+F+LR
Sbjct: 1553 EDFKLR 1557

[Close]

BLAST of PI0000866 vs. ExPASy TrEMBL
Match: A0A5D3C8Z6 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold265G00760 PE=4 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 1.0e-08
Identity = 38/66 (57.58%), Postives = 47/66 (71.21%), Query Frame = 0


Query: 5   SAQSLSDFYGKGTICKGPHVARECPNRTAFYAFQAALASDSDDKNGRAEDEDVQIEG-DY 64
           S Q+LS+      ICKGPH+ARECPN+TAF AFQA+L SDSD++  + E E  Q E  D 
Sbjct: 379 SNQNLSNRPLSCFICKGPHMARECPNKTAFNAFQASLTSDSDNQQSQTEGEVNQTEEVDN 438

Query: 65  PRVGAL 70
           PR+GAL
Sbjct: 439 PRMGAL 444

HSP 2 Score: 109.4 bits (272), Expect = 1.2e-20
Identity = 53/66 (80.30%), Postives = 60/66 (90.91%), Query Frame = 0


Query: 69  LSLNQKEEDRDVEEILTDWVRKGRRPTRRIHEYLIKWKNLPVEETSWEHVEDLEAWKQKI 128
           + L+QK ED+DVEEIL + VRKGRRPTRRIHEYL+KWKNLPVEE+SWE VEDLEAW QKI
Sbjct: 196 IDLSQK-EDKDVEEILAERVRKGRRPTRRIHEYLVKWKNLPVEESSWERVEDLEAWTQKI 255

Query: 129 EEFQLR 135
           EEF+LR
Sbjct: 256 EEFKLR 260

[Close]

BLAST of PI0000866 vs. ExPASy TrEMBL
Match: A0A5A7VG74 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold25954G00010 PE=4 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 1.2e-20
Identity = 52/66 (78.79%), Postives = 60/66 (90.91%), Query Frame = 0


Query: 69  LSLNQKEEDRDVEEILTDWVRKGRRPTRRIHEYLIKWKNLPVEETSWEHVEDLEAWKQKI 128
           + LNQK ED++VEEIL + VR+GRRP RRIHEYL+KWKNLPVEETSWE VEDLEAWKQKI
Sbjct: 97  IDLNQK-EDKEVEEILAERVRRGRRPARRIHEYLVKWKNLPVEETSWERVEDLEAWKQKI 156

Query: 129 EEFQLR 135
           E+F+LR
Sbjct: 157 EDFKLR 161

[Close]

BLAST of PI0000866 vs. NCBI nr
Match: KAA0057858.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 110.9 bits (276), Expect = 8.5e-21
Identity = 53/66 (80.30%), Postives = 61/66 (92.42%), Query Frame = 0


Query: 69  LSLNQKEEDRDVEEILTDWVRKGRRPTRRIHEYLIKWKNLPVEETSWEHVEDLEAWKQKI 128
           + L+QK ED+DVEEIL + VR+GRRPTRRIHEYL+KWKNLPVEETSWE VEDLEAWKQKI
Sbjct: 469 IDLSQK-EDKDVEEILAERVRRGRRPTRRIHEYLVKWKNLPVEETSWERVEDLEAWKQKI 528

Query: 129 EEFQLR 135
           E+F+LR
Sbjct: 529 EDFKLR 533

[Close]

BLAST of PI0000866 vs. NCBI nr
Match: TYJ98540.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 110.9 bits (276), Expect = 8.5e-21
Identity = 53/66 (80.30%), Postives = 61/66 (92.42%), Query Frame = 0


Query: 69  LSLNQKEEDRDVEEILTDWVRKGRRPTRRIHEYLIKWKNLPVEETSWEHVEDLEAWKQKI 128
           + L+QK ED+DVEEIL + VR+GRRPTRRIHEYL+KWKNLPVEETSWE VEDLEAWKQKI
Sbjct: 187 IDLSQK-EDKDVEEILAERVRRGRRPTRRIHEYLVKWKNLPVEETSWERVEDLEAWKQKI 246

Query: 129 EEFQLR 135
           E+F+LR
Sbjct: 247 EDFKLR 251

[Close]

BLAST of PI0000866 vs. NCBI nr
Match: TYK07955.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 109.8 bits (273), Expect = 1.9e-20
Identity = 53/66 (80.30%), Postives = 60/66 (90.91%), Query Frame = 0


Query: 69   LSLNQKEEDRDVEEILTDWVRKGRRPTRRIHEYLIKWKNLPVEETSWEHVEDLEAWKQKI 128
            + L+QK ED+DVEEIL + VR+GRRPTRRIHEYLIKWKNLPVEETSWE VEDLE WKQKI
Sbjct: 1493 IDLSQK-EDKDVEEILAERVRRGRRPTRRIHEYLIKWKNLPVEETSWERVEDLEVWKQKI 1552

Query: 129  EEFQLR 135
            E+F+LR
Sbjct: 1553 EDFKLR 1557

[Close]

BLAST of PI0000866 vs. NCBI nr
Match: TYK07955.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 69.7 bits (169), Expect = 2.2e-08
Identity = 38/66 (57.58%), Postives = 47/66 (71.21%), Query Frame = 0


Query: 5   SAQSLSDFYGKGTICKGPHVARECPNRTAFYAFQAALASDSDDKNGRAEDEDVQIEG-DY 64
           S Q+LS+      ICKGPH+ARECPN+TAF AFQA+L SDSD++  + E E  Q E  D 
Sbjct: 379 SNQNLSNRPLSCFICKGPHMARECPNKTAFNAFQASLTSDSDNQQSQTEGEVNQTEEVDN 438

Query: 65  PRVGAL 70
           PR+GAL
Sbjct: 439 PRMGAL 444

HSP 2 Score: 109.4 bits (272), Expect = 2.5e-20
Identity = 52/66 (78.79%), Postives = 60/66 (90.91%), Query Frame = 0


Query: 69  LSLNQKEEDRDVEEILTDWVRKGRRPTRRIHEYLIKWKNLPVEETSWEHVEDLEAWKQKI 128
           + LNQK ED++VEEIL + VR+GRRP RRIHEYL+KWKNLPVEETSWE VEDLEAWKQKI
Sbjct: 97  IDLNQK-EDKEVEEILAERVRRGRRPARRIHEYLVKWKNLPVEETSWERVEDLEAWKQKI 156

Query: 129 EEFQLR 135
           E+F+LR
Sbjct: 157 EDFKLR 161

[Close]

BLAST of PI0000866 vs. NCBI nr
Match: TYK04336.1 (2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase [Cucumis melo var. makuwa])

HSP 1 Score: 109.4 bits (272), Expect = 2.5e-20
Identity = 52/66 (78.79%), Postives = 61/66 (92.42%), Query Frame = 0


Query: 69  LSLNQKEEDRDVEEILTDWVRKGRRPTRRIHEYLIKWKNLPVEETSWEHVEDLEAWKQKI 128
           + L+QK ED+DVEEIL + VR+GRRPTRRIHEYL+KWKNLPVEETSWE VEDLEAWKQKI
Sbjct: 32  IDLSQK-EDKDVEEILAERVRRGRRPTRRIHEYLVKWKNLPVEETSWERVEDLEAWKQKI 91

Query: 129 EEFQLR 135
           ++F+LR
Sbjct: 92  KDFKLR 96

The following BLAST results are available for this feature:

BLAST of PI0000866 vs.


Match Name	E-value	Identity	Description

BLAST of PI0000866 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7URR9	4.1e-21	80.30	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold27...	[more]
A0A5D3BFH0	4.1e-21	80.30	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35...	[more]
A0A5D3C8Z6	9.1e-21	80.30	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold26...	[more]
A0A5D3C8Z6	1.0e-08	57.58	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold26...	[more]
A0A5A7VG74	1.2e-20	78.79	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold25...	[more]

BLAST of PI0000866 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0057858.1	8.5e-21	80.30	reverse transcriptase [Cucumis melo var. makuwa]	[more]
TYJ98540.1	8.5e-21	80.30	reverse transcriptase [Cucumis melo var. makuwa]	[more]
TYK07955.1	1.9e-20	80.30	reverse transcriptase [Cucumis melo var. makuwa]	[more]
TYK07955.1	2.2e-08	57.58	reverse transcriptase [Cucumis melo var. makuwa]	[more]
TYK04336.1	2.5e-20	78.79	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase [Cucumis melo var. maku...	[more]

BLAST of PI0000866 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 121..134
None	No IPR available	GENE3D	2.40.50.40		coord: 71..134 e-value: 5.7E-11 score: 43.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 42..71
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 46..61
None	No IPR available	CDD	cd00024	CD_CSD	coord: 80..132 e-value: 6.15142E-9 score: 46.7013
IPR023780	Chromo domain	PFAM	PF00385	Chromo	coord: 79..132 e-value: 2.4E-10 score: 40.2
IPR000953	Chromo/chromo shadow domain	PROSITE	PS50013	CHROMO_2	coord: 78..134 score: 12.648001
IPR016197	Chromo-like domain superfamily	SUPERFAMILY	54160	Chromo domain-like	coord: 74..132

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0000866.1	PI0000866.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015074	DNA integration
biological_process	GO:0006278	RNA-dependent DNA biosynthetic process
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0003964	RNA-directed DNA polymerase activity