PI0000809 (gene) Melon (PI 482460) v1

Overview
NamePI0000809
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionMannan endo-1,4-beta-mannosidase
Locationchr04: 29118057 .. 29120729 (+)
RNA-Seq ExpressionPI0000809
SyntenyPI0000809
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGCTTCGGATTTACTTTTTGATTCTACTTCTTCTACCTCTAGTGCTTCTAGCAAAAGCAGATGATGGGTTTGTAAGAACTAAAGGGCAACAACTGATTCTGAATGAAACTCCATTTTACGCCAATGGGTTCAATGCCTATTGGTTAATGTACTTCGCTTCAGACCCATCACAGAGACCCAAAGTCTCCTCTGCTTTTCAACAAGCCGTCAATCACGGTCTCTCAATTGGAAGAACTTGGGCCTTCAACGATGGCGGATACAGCCCTTTACAGTATTCCCCAGGACAATACAATGAGAAGATGTTCCAGGTTCTTTGATTCCATTGATTTTTGTATTTGCTCTGTTTTTTTTTTTTTTTTTTTTTTTTCATCTTTTGTTAAGTGAGAAATGGGTTTTTGTTAGGGATTGGATTTTGTGGTAGCGGAAGCAAGGAAATATGGGATTAAACTGATACTGAGTATGGTGAATAATTATGAAAGTATGGGAGGGAAAAAAACAGTATGTGGAATGGGCGAGAAGCCAGGGGCAGGCTATTTCTTCTGAGGATGAGTTCTTCACTAATCCTGTTGTTAAAGGGTTCTACAAGAACCATATTAAGGTAAAAATCAGAATGCCTAATCAAATTTCAAGTTGTGATTTCTAAACTTTCATTTTGGATTTTTCTGTTTTGTTGGTGGAATTTGGTTGAAGCTTTGAAATGGGGTTGTGAATGATTTAACGTTTATGCAGTGAGGTTGTGAGGGGGATTGGCTGGTGAAAGTGGAAATGGAGTAGTTGGAGTTTCGGAGAAGAAAGAGTGGTTGGGAATTGGAATTAGGTCATTTACAGAGGAGAGTAATTATGAAAATTTATTTATTTTTACCTTTTTCCATAAGTTTTTTCTGAAATGATTTCCATTGGTGGACGTTCTTATTTCATTACGTTGAAGATTGAGCTTACCCAAATTTTATTTTCAACTGATTGAAACATGAATTTCAATAAAGGTGAAATTTTGTTAATTTGATTTATATTTTTTTAAAGAAAAGACAAACATTTTTGCCTCAAGACTTCATTGTGTGGGTAGTAAGTTCATGCTTTTTAATGGGTTGGGTTTGAATTTTTTTTTAAAAAAAACCATTTGGCCTTCAATCTCAATATTTAAAATACTTTATTAGTTGAATTTGCTGAGCTTTATCATTTGTTTTATATATGTAATATTTTAGGATCAAAAGTGACTTTATTTACGTTGTTGACCAAATTGCCCATTATAGGGAGCTACTAGTTAAAAGTCGATATTGTGACTTTATTTTGATTTCCACCTTTTATGGAACCTTTTTGAAGTTTTTTTCTTTTTTTTTTATTTTTTATTTTCAAACTTGACAGGTAATAAATTAGAAATTGGTATGAAATTCAATGATAAAATCCATTTGCTTTATAGTCTCCTTCTTTGCACCAATTACCATATCTATCAAATTGCAAGCTATGCAAACGTGAGCATAGCTACTTAGTTACAAAATCACTCTTGGATTCAAACCCTTTTACTCAATATGTAGAAGAAAGCCGTGATTTATAAATACTGATAATTTTGTTTGAAAAATACTTTAAAATGTATATAGAGCATTTTGACAAGAGTCAATAGCATTACTGGAGTAGCTTATAAGGATGATCCAACAATAATGGCTTGGGAACTCATGAACGAGGCCAGATGCCCTTCAGATCCTTCAGGAAACACCATTCAGGTTCTCACTACAGATTTCCTCCCTTCTTCCCTGCTCTTAATTTCAATCTCACTCATTCCAAAACCAATTTTAAGTATGGTTTGAATTAACTTTTCAAGTATTTAAATAAGTGTTTATAAACATTTAGAAAGTTAATCCAAACGGACACTTAATGTAAATTTTGTCATTTTAAGTTGACAAAGTTGTATGAATGAAATGAAATGATGGGCAGGCATGGATTAAAGAAATGGCATCATATTTGAAATCAATAGATGGGAAACACCTATTAGAAGCTGGTTTGGAAGGTTTCTATGGGCAATCAAAGTATCAATGGAATCCAAACTTTCAAGTGGGAACAGATTTCATTGCAAACAATCAAATACCTGAAATTGACTTTGCTACAGTCCATTCATATCCTGACCAATGGTAAAAACAAAATTTCCCCTATTTGATTCTTTTTGGGATCTTTAATCTCCAACAAGCTAAAATGAAATGGGTGATTTCTACTTATGTTGTAGGTTATCTGGTTCTAGTTACGAGAATCAGCTCTCGTTCCTCAACACTTGGCTTAATGATCACATCCAAGATGCACAGAACATTCTGCATAAGCCAGTTCTTTTTGCTGAGTTTGGAAAATCTACAAAGTATTCTGGCGCTGATCAAAGGGATCAGCTTTTCAATGCTGTTTACTCGGCTGTGTATTCGTCCGCTCGAGGGGGTGGTGCAGCCATCGGTGGAATGTTCTGGCAACTTCTAGTTGAAGGGATGGATTCTTTCCGAGATGGGTATGAAGTGGTACTGAGCGAGAGCCCCTCGACTGCAAATTTGATCACCCAGGAGTCTAGAAGGCTGATTCACATCAGAAGGATGTATGCCAAACTCAGAAACATTGAGAAGTGGAAGAGAGCAAAGGAGATGAAAAAAGCACAGTGGGAGGCCTCAAAGGGAGGCAACAATAGTCCTGGAAATTAA

mRNA sequence

ATGAAGCTTCGGATTTACTTTTTGATTCTACTTCTTCTACCTCTAGTGCTTCTAGCAAAAGCAGATGATGGGTTTGTAAGAACTAAAGGGCAACAACTGATTCTGAATGAAACTCCATTTTACGCCAATGGGTTCAATGCCTATTGGTTAATGTACTTCGCTTCAGACCCATCACAGAGACCCAAAGTCTCCTCTGCTTTTCAACAAGCCGTCAATCACGGTCTCTCAATTGGAAGAACTTGGGCCTTCAACGATGGCGGATACAGCCCTTTACAGTATTCCCCAGGACAATACAATGAGAAGATGTTCCAGGGAAAAAAACAGTATGTGGAATGGGCGAGAAGCCAGGGGCAGGCTATTTCTTCTGAGGATGAGTTCTTCACTAATCCTGTTGTTAAAGGGTTCTACAAGAACCATATTAAGAGCATTTTGACAAGAGTCAATAGCATTACTGGAGTAGCTTATAAGGATGATCCAACAATAATGGCTTGGGAACTCATGAACGAGGCCAGATGCCCTTCAGATCCTTCAGGAAACACCATTCAGGCATGGATTAAAGAAATGGCATCATATTTGAAATCAATAGATGGGAAACACCTATTAGAAGCTGGTTTGGAAGGTTTCTATGGGCAATCAAAGTATCAATGGAATCCAAACTTTCAAGTGGGAACAGATTTCATTGCAAACAATCAAATACCTGAAATTGACTTTGCTACAGTCCATTCATATCCTGACCAATGGTTATCTGGTTCTAGTTACGAGAATCAGCTCTCGTTCCTCAACACTTGGCTTAATGATCACATCCAAGATGCACAGAACATTCTGCATAAGCCAGTTCTTTTTGCTGAGTTTGGAAAATCTACAAAGTATTCTGGCGCTGATCAAAGGGATCAGCTTTTCAATGCTGTTTACTCGGCTGTGTATTCGTCCGCTCGAGGGGGTGGTGCAGCCATCGGTGGAATGTTCTGGCAACTTCTAGTTGAAGGGATGGATTCTTTCCGAGATGGGTATGAAGTGGTACTGAGCGAGAGCCCCTCGACTGCAAATTTGATCACCCAGGAGTCTAGAAGGCTGATTCACATCAGAAGGATGTATGCCAAACTCAGAAACATTGAGAAGTGGAAGAGAGCAAAGGAGATGAAAAAAGCACAGTGGGAGGCCTCAAAGGGAGGCAACAATAGTCCTGGAAATTAA

Coding sequence (CDS)

ATGAAGCTTCGGATTTACTTTTTGATTCTACTTCTTCTACCTCTAGTGCTTCTAGCAAAAGCAGATGATGGGTTTGTAAGAACTAAAGGGCAACAACTGATTCTGAATGAAACTCCATTTTACGCCAATGGGTTCAATGCCTATTGGTTAATGTACTTCGCTTCAGACCCATCACAGAGACCCAAAGTCTCCTCTGCTTTTCAACAAGCCGTCAATCACGGTCTCTCAATTGGAAGAACTTGGGCCTTCAACGATGGCGGATACAGCCCTTTACAGTATTCCCCAGGACAATACAATGAGAAGATGTTCCAGGGAAAAAAACAGTATGTGGAATGGGCGAGAAGCCAGGGGCAGGCTATTTCTTCTGAGGATGAGTTCTTCACTAATCCTGTTGTTAAAGGGTTCTACAAGAACCATATTAAGAGCATTTTGACAAGAGTCAATAGCATTACTGGAGTAGCTTATAAGGATGATCCAACAATAATGGCTTGGGAACTCATGAACGAGGCCAGATGCCCTTCAGATCCTTCAGGAAACACCATTCAGGCATGGATTAAAGAAATGGCATCATATTTGAAATCAATAGATGGGAAACACCTATTAGAAGCTGGTTTGGAAGGTTTCTATGGGCAATCAAAGTATCAATGGAATCCAAACTTTCAAGTGGGAACAGATTTCATTGCAAACAATCAAATACCTGAAATTGACTTTGCTACAGTCCATTCATATCCTGACCAATGGTTATCTGGTTCTAGTTACGAGAATCAGCTCTCGTTCCTCAACACTTGGCTTAATGATCACATCCAAGATGCACAGAACATTCTGCATAAGCCAGTTCTTTTTGCTGAGTTTGGAAAATCTACAAAGTATTCTGGCGCTGATCAAAGGGATCAGCTTTTCAATGCTGTTTACTCGGCTGTGTATTCGTCCGCTCGAGGGGGTGGTGCAGCCATCGGTGGAATGTTCTGGCAACTTCTAGTTGAAGGGATGGATTCTTTCCGAGATGGGTATGAAGTGGTACTGAGCGAGAGCCCCTCGACTGCAAATTTGATCACCCAGGAGTCTAGAAGGCTGATTCACATCAGAAGGATGTATGCCAAACTCAGAAACATTGAGAAGTGGAAGAGAGCAAAGGAGATGAAAAAAGCACAGTGGGAGGCCTCAAAGGGAGGCAACAATAGTCCTGGAAATTAA

Protein sequence

MKLRIYFLILLLLPLVLLAKADDGFVRTKGQQLILNETPFYANGFNAYWLMYFASDPSQRPKVSSAFQQAVNHGLSIGRTWAFNDGGYSPLQYSPGQYNEKMFQGKKQYVEWARSQGQAISSEDEFFTNPVVKGFYKNHIKSILTRVNSITGVAYKDDPTIMAWELMNEARCPSDPSGNTIQAWIKEMASYLKSIDGKHLLEAGLEGFYGQSKYQWNPNFQVGTDFIANNQIPEIDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQNILHKPVLFAEFGKSTKYSGADQRDQLFNAVYSAVYSSARGGGAAIGGMFWQLLVEGMDSFRDGYEVVLSESPSTANLITQESRRLIHIRRMYAKLRNIEKWKRAKEMKKAQWEASKGGNNSPGN
Homology
BLAST of PI0000809 vs. ExPASy Swiss-Prot
Match: Q9FJZ3 (Mannan endo-1,4-beta-mannosidase 7 OS=Arabidopsis thaliana OX=3702 GN=MAN7 PE=2 SV=1)

HSP 1 Score: 498.0 bits (1281), Expect = 9.6e-140
Identity = 241/388 (62.11%), Postives = 292/388 (75.26%), Query Frame = 0

Query: 24  GFVRTKGQQLILNETPFYANGFNAYWLMYFASDPSQRPKVSSAFQQAVNHGLSIGRTWAF 83
           GFVRTKG Q  LN  P+YANGFNAYWLMY ASDPSQR K+S+AFQ A  HGL++ RTWAF
Sbjct: 30  GFVRTKGVQFSLNGYPYYANGFNAYWLMYVASDPSQRSKISTAFQDASRHGLTVARTWAF 89

Query: 84  NDGGYSPLQYSPGQYNEKMFQ----------------------------GKKQYVEWARS 143
           +DGGY  LQYSPG YNE MFQ                            G+KQYV+WARS
Sbjct: 90  SDGGYRALQYSPGSYNEDMFQGLDFALAEARRHGIKIILSFANNYESFGGRKQYVDWARS 149

Query: 144 QGQAISSEDEFFTNPVVKGFYKNHIKSILTRVNSITGVAYKDDPTIMAWELMNEARCPSD 203
           +G+ +SSED+FFT+ +VK FYKNHIK++L R N+ T V YKDDPTIMAWELMNE RCPSD
Sbjct: 150 RGRPVSSEDDFFTDSLVKDFYKNHIKAVLNRFNTFTKVHYKDDPTIMAWELMNEPRCPSD 209

Query: 204 PSGNTIQAWIKEMASYLKSIDGKHLLEAGLEGFYGQSKYQ---WNPNFQVGTDFIANNQI 263
           PSG  IQAWI EMA+++KS+D  HLLEAGLEGFYGQS  Q    NP  Q GTDFIANN+I
Sbjct: 210 PSGRAIQAWITEMAAHVKSLDRNHLLEAGLEGFYGQSSPQSKTLNPPGQFGTDFIANNRI 269

Query: 264 PEIDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQNILHKPVLFAEFGKSTKYSG 323
           P IDF TVHSYPD+W   SS ++Q+ FLN WL+ HIQDAQN+LHKP++ AEFGKS K  G
Sbjct: 270 PGIDFVTVHSYPDEWFPDSSEQSQMDFLNKWLDAHIQDAQNVLHKPIILAEFGKSMKKPG 329

Query: 324 --ADQRDQLFNAVYSAVYSSARGGGAAIGGMFWQLLVEGMDSFRDGYEVVLSESPSTANL 379
               QRD +FN VYS +Y SA+ GGAA GG+FWQLLV G+D+F+DGY ++LS+S ST N+
Sbjct: 330 YTPAQRDIVFNTVYSKIYGSAKRGGAAAGGLFWQLLVNGIDNFQDGYGIILSQSSSTVNV 389

BLAST of PI0000809 vs. ExPASy Swiss-Prot
Match: Q0JKM9 (Mannan endo-1,4-beta-mannosidase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=MAN1 PE=2 SV=2)

HSP 1 Score: 453.4 bits (1165), Expect = 2.7e-126
Identity = 224/385 (58.18%), Postives = 275/385 (71.43%), Query Frame = 0

Query: 24  GFVRTKGQQLILNETPFYANGFNAYWLMYFASDPSQRPKVSSAFQQAVNHGLSIGRTWAF 83
           GFVR +G + +L+  P+YANGFNAYWLM  A+DPSQR KVS+A  +A  HGL++ RTWAF
Sbjct: 36  GFVRAQGTRFVLDGNPYYANGFNAYWLMLLAADPSQRGKVSAALGEAAGHGLTVARTWAF 95

Query: 84  ND-GGYSPLQYSPGQYNEKMFQ----------------------------GKKQYVEWAR 143
           +D GG + LQ SPG YNE  F+                            G+KQYV WAR
Sbjct: 96  SDGGGGNALQLSPGNYNENTFKGLDFVLSEARKYGIKVILSLVDNYDSFGGRKQYVNWAR 155

Query: 144 SQGQAISSEDEFFTNPVVKGFYKNHIKSILTRVNSITGVAYKDDPTIMAWELMNEARCPS 203
           +QGQ I S+DEFFTNPVVKGFYKNH+K++LTR N+ITGVAY+DDPTI+AWELMNE RC S
Sbjct: 156 AQGQGIGSDDEFFTNPVVKGFYKNHVKTVLTRKNTITGVAYRDDPTILAWELMNEPRCQS 215

Query: 204 DPSGNTIQAWIKEMASYLKSIDGKHLLEAGLEGFYGQSK----YQWNPN-FQVGTDFIAN 263
           D SG T+Q+WI EMA+++KSID  H+LE GLEGFYG S        NP+ +Q+GTDFIAN
Sbjct: 216 DLSGRTVQSWITEMAAHVKSIDRNHMLEVGLEGFYGASSPSRIAAVNPSGYQLGTDFIAN 275

Query: 264 NQIPEIDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQNILHKPVLFAEFGKSTK 323
           NQ+P IDFATVHSYPDQWLSG   + QL F+  WL+ HI DAQ +L KP+L AEFGKS K
Sbjct: 276 NQVPGIDFATVHSYPDQWLSGKDDQAQLGFMGRWLDAHIADAQAVLRKPLLIAEFGKSWK 335

Query: 324 YSG--ADQRDQLFNAVYSAVYSSARGGGAAIGGMFWQLLVEGMDSFRDGYEVVLSESPST 373
             G  + QRD L+  VY+ +Y SAR GGA +GG+FWQLLV GMDS+RDGYEVV  E+PST
Sbjct: 336 DPGYSSGQRDALYGTVYAKIYESARRGGATVGGLFWQLLVPGMDSYRDGYEVVFGETPST 395

BLAST of PI0000809 vs. ExPASy Swiss-Prot
Match: Q6Z310 (Putative mannan endo-1,4-beta-mannosidase 9 OS=Oryza sativa subsp. japonica OX=39947 GN=MAN9 PE=2 SV=2)

HSP 1 Score: 406.4 bits (1043), Expect = 3.8e-112
Identity = 194/369 (52.57%), Postives = 249/369 (67.48%), Query Frame = 0

Query: 21  ADDGFVRTKGQQLILNETPFYANGFNAYWLMYFASDPSQRPKVSSAFQQAVNHGLSIGRT 80
           A+  F R  G +  +   PFY+NGFNAYWLMY ASDP  R K +   QQA +   ++ RT
Sbjct: 35  ANGQFARASGTRFTVGGRPFYSNGFNAYWLMYMASDPGDRSKAAGVLQQAASLRATLVRT 94

Query: 81  WAFNDGGYSPLQYSPGQYNEKMFQ----------------------------GKKQYVEW 140
           WAF+DGGY PLQ SPG YNE MF                             GKKQYV+W
Sbjct: 95  WAFSDGGYRPLQKSPGVYNEDMFMGLDFVIAEAKKRGLYLILSLVNNWDGFGGKKQYVQW 154

Query: 141 ARSQGQAISSEDEFFTNPVVKGFYKNHIKSILTRVNSITGVAYKDDPTIMAWELMNEARC 200
           AR QG  + S+D+FF + V K FYKNH  ++LTRVN ITGVAYKDDPTI AWEL+NE RC
Sbjct: 155 ARDQGHNLGSDDDFFRSDVTKQFYKNH--AVLTRVNKITGVAYKDDPTIFAWELINEPRC 214

Query: 201 PSDPSGNTIQAWIKEMASYLKSIDGKHLLEAGLEGFYGQSKYQ-WNPNFQVGTDFIANNQ 260
            SD SG T+QAW+ EMA Y+KS+D  H++E GLEGFYG+S ++ +NP + VGTDFIANN 
Sbjct: 215 QSDLSGKTLQAWVTEMAGYVKSVDPNHMVEIGLEGFYGESMHKNFNPGYTVGTDFIANNL 274

Query: 261 IPEIDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQNILHKPVLFAEFGKSTKYS 320
           +P +DFAT+HSYPDQW+SG+S + Q++F+  W+ DHI+D+  +L KP+L  EFG S + +
Sbjct: 275 VPAVDFATIHSYPDQWVSGASSDEQVAFMRKWMADHIRDSAAVLRKPLLVTEFGWSARSN 334

Query: 321 G--ADQRDQLFNAVYSAVYSSARGGGAAIGGMFWQLLVEGMDSFRDGYEVVLSESPSTAN 359
           G     RD  F  VY AVY+SAR GGA  GG+FWQ++  GM+S+ DGYEVVL  S STA+
Sbjct: 335 GYTVAARDAYFRTVYDAVYASAREGGACAGGLFWQVMAPGMESWTDGYEVVLERSKSTAD 394

BLAST of PI0000809 vs. ExPASy Swiss-Prot
Match: Q8L5J1 (Mannan endo-1,4-beta-mannosidase 4 OS=Solanum lycopersicum OX=4081 GN=MAN4 PE=1 SV=2)

HSP 1 Score: 395.6 bits (1015), Expect = 6.7e-109
Identity = 201/389 (51.67%), Postives = 258/389 (66.32%), Query Frame = 0

Query: 5   IYFLILLLLP----LVLLAKADDGFVRTKGQQLILNETPFYANGFNAYWLMYFASDPSQR 64
           I+  IL++ P        A +++ FV T G    LN    Y NGFNAYWLMY A DPS R
Sbjct: 8   IFVAILIIFPNEFSKPTRAFSNNNFVYTDGTHFALNGKSLYINGFNAYWLMYIAYDPSTR 67

Query: 65  PKVSSAFQQAVNHGLSIGRTWAFNDGGYSPLQYSPGQYNEKMFQ---------------- 124
            KV++ FQQA  + +++ RTWAF+ GG  PLQ +PG YNE+MFQ                
Sbjct: 68  IKVTNTFQQASKYKMNVARTWAFSHGGSRPLQSAPGVYNEQMFQGLDFVISEAKKYGIHL 127

Query: 125 ------------GKKQYVEWARSQGQAISSEDEFFTNPVVKGFYKNHIKSILTRVNSITG 184
                       GKKQYVEWA  +GQ ++S+D+FFTNP+VKGFYKN++K +LTRVN+IT 
Sbjct: 128 IMSLVNNWDAFGGKKQYVEWAVQRGQKLTSDDDFFTNPMVKGFYKNNVKVVLTRVNTITK 187

Query: 185 VAYKDDPTIMAWELMNEARCPSDPSGNTIQAWIKEMASYLKSIDGKHLLEAGLEGFYGQS 244
           VAYKDDPTI++WEL+NE RCPSD SG T Q W+ EMA YLKSID  HLLE GLEGFYG  
Sbjct: 188 VAYKDDPTILSWELINEPRCPSDLSGKTFQNWVLEMAGYLKSIDSNHLLEIGLEGFYGND 247

Query: 245 KYQWNPNFQV-GTDFIANNQIPEIDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDA 304
             Q+NPN  + GT+FI+NNQ+  IDF T+H YP+QWL G + E Q  + + W+  HI D+
Sbjct: 248 MRQYNPNSYIFGTNFISNNQVQGIDFTTIHMYPNQWLPGLTQEAQDKWASQWIQVHIDDS 307

Query: 305 QNILHKPVLFAEFGKSTKYSG--ADQRDQLFNAVYSAVYSSARGGGAAIGGMFWQLLVEG 359
           + +L KP+L AEFGKSTK  G    +RD  F  +Y  +++ A+ GG   GG+FWQ+L +G
Sbjct: 308 K-MLKKPLLIAEFGKSTKTPGYTVAKRDNYFEKIYGTIFNCAKSGGPCGGGLFWQVLGQG 367

BLAST of PI0000809 vs. ExPASy Swiss-Prot
Match: Q9SG94 (Mannan endo-1,4-beta-mannosidase 3 OS=Arabidopsis thaliana OX=3702 GN=MAN3 PE=2 SV=1)

HSP 1 Score: 385.6 bits (989), Expect = 6.9e-106
Identity = 193/404 (47.77%), Postives = 260/404 (64.36%), Query Frame = 0

Query: 1   MKLRIYFLILLLL------PLVLLAKADDGFVRTKGQQLILNETPFYANGFNAYWLMYFA 60
           MK   + ++L +L       L + + + DGFV  KG Q ILN  PFYANGFNAYWL Y A
Sbjct: 1   MKCLCFVVLLAILIAQNSSDLGVKSASSDGFVSRKGVQFILNGKPFYANGFNAYWLAYEA 60

Query: 61  SDPSQRPKVSSAFQQAVNHGLSIGRTWAFNDGGYSPLQYSPGQYNEKMFQ---------- 120
           +D + R K++  FQ A  H L+I RTW F DGGY  LQ +PG Y+EK FQ          
Sbjct: 61  TDSTTRFKITYVFQNATIHDLTIVRTWGFRDGGYRALQIAPGVYDEKTFQGLDFAIAEAK 120

Query: 121 ------------------GKKQYVEWARSQGQAISSEDEFFTNPVVKGFYKNHIKSILTR 180
                             G+KQYV+WA++ GQ +SS+D+F+TNP+VK +YKNH+K+++ R
Sbjct: 121 RLGIKMIITFVNNYSDFGGRKQYVDWAKNTGQNVSSDDDFYTNPLVKQYYKNHVKTMVNR 180

Query: 181 VNSITGVAYKDDPTIMAWELMNEARCPSDPSGNTIQAWIKEMASYLKSIDGKHLLEAGLE 240
           VN+ T V YKD+PTIM WELMNE +C +DPSG T+ AW+ EMA Y+KS+D KHLL  GLE
Sbjct: 181 VNTFTKVEYKDEPTIMGWELMNEPQCRADPSGKTLTAWMNEMALYVKSVDSKHLLSTGLE 240

Query: 241 GFYGQSKYQWNPNFQ------VGTDFIANNQIPEIDFATVHSYPDQWLSGSSYENQLSFL 300
           GFYG S  Q   +        +GTDFIAN+++  IDFA++HSYPD W      +++L+ L
Sbjct: 241 GFYGDSSPQRKTSLNPVAANVLGTDFIANHKLDAIDFASIHSYPDLWFPNLDEKSRLNLL 300

Query: 301 NTWLNDHIQDAQNILHKPVLFAEFGKSTKYSG--ADQRDQLFNAVYSAVYSSARGGGAAI 360
             WL  H++DAQNIL KP++  EFGK T   G    QRD +FNA +  +Y SA  GG A 
Sbjct: 301 RKWLECHLEDAQNILKKPLILGEFGKPTNTPGYTQAQRDAVFNATFDTIYESAEKGGPAA 360

Query: 361 GGMFWQLLVEGMDSFRDGYEVVLSESPSTANLITQESRRLIHIR 363
           G +FW ++ +GM++F+D   +VLSE+ +T N+IT+ESR+L  IR
Sbjct: 361 GALFWHVISDGMNNFKDPLSIVLSENSTTVNIITEESRKLGLIR 404

BLAST of PI0000809 vs. ExPASy TrEMBL
Match: A0A5A7TIV8 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold242G00810 PE=3 SV=1)

HSP 1 Score: 779.2 bits (2011), Expect = 7.9e-222
Identity = 383/397 (96.47%), Postives = 387/397 (97.48%), Query Frame = 0

Query: 1   MKLRIYFLILLLLPLVLLAKADDGFVRTKGQQLILNETPFYANGFNAYWLMYFASDPSQR 60
           MKL I   ILL LPLVLLAKADDGFVRTKGQQLILN TPFYANGFNAYWLMYFASDPS +
Sbjct: 1   MKLGISIFILLFLPLVLLAKADDGFVRTKGQQLILNGTPFYANGFNAYWLMYFASDPSLK 60

Query: 61  PKVSSAFQQAVNHGLSIGRTWAFNDGGYSPLQYSPGQYNEKMFQGKKQYVEWARSQGQAI 120
           PKVSSAFQQAV+HGLSIGRTWAFNDGGYSPLQYSPGQYNEK+  GKKQYVEWARSQGQAI
Sbjct: 61  PKVSSAFQQAVDHGLSIGRTWAFNDGGYSPLQYSPGQYNEKIMGGKKQYVEWARSQGQAI 120

Query: 121 SSEDEFFTNPVVKGFYKNHIKSILTRVNSITGVAYKDDPTIMAWELMNEARCPSDPSGNT 180
           SSEDEFFTNPVVKGFYKNHIKSILTRVNSITGVAYKDDPTIMAWELMNEARCPSDPSGNT
Sbjct: 121 SSEDEFFTNPVVKGFYKNHIKSILTRVNSITGVAYKDDPTIMAWELMNEARCPSDPSGNT 180

Query: 181 IQAWIKEMASYLKSIDGKHLLEAGLEGFYGQSKYQWNPNFQVGTDFIANNQIPEIDFATV 240
           IQAWIKEMASYLKSIDGKHLLEAGLEGFYGQSKYQWNPNFQVGTDFIANNQIPEIDFATV
Sbjct: 181 IQAWIKEMASYLKSIDGKHLLEAGLEGFYGQSKYQWNPNFQVGTDFIANNQIPEIDFATV 240

Query: 241 HSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQNILHKPVLFAEFGKSTKYSGADQRDQLF 300
           HSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQNILHKPVLFAEFGKS KYSGADQRDQLF
Sbjct: 241 HSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQNILHKPVLFAEFGKSIKYSGADQRDQLF 300

Query: 301 NAVYSAVYSSARGGGAAIGGMFWQLLVEGMDSFRDGYEVVLSESPSTANLITQESRRLIH 360
           NAVYSAVYSSARGGGAAIGGMFWQLLVEGMDSFRDGYEVVLSESPSTANLITQESRRLIH
Sbjct: 301 NAVYSAVYSSARGGGAAIGGMFWQLLVEGMDSFRDGYEVVLSESPSTANLITQESRRLIH 360

Query: 361 IRRMYAKLRNIEKWKRAKEMKKAQWEASKGGNNSPGN 398
           IRRMYAKLRNIEKWKRAKEMKKAQWEAS+GGNNSPGN
Sbjct: 361 IRRMYAKLRNIEKWKRAKEMKKAQWEASRGGNNSPGN 397

BLAST of PI0000809 vs. ExPASy TrEMBL
Match: E5GCI6 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 770.8 bits (1989), Expect = 2.8e-219
Identity = 386/425 (90.82%), Postives = 389/425 (91.53%), Query Frame = 0

Query: 1   MKLRIYFLILLLLPLVLLAKADDGFVRTKGQQLILNETPFYANGFNAYWLMYFASDPSQR 60
           MKL I   ILL LPLVLLAKADDGFVRTKGQQLILN TPFYANGFNAYWLMYFASDPS +
Sbjct: 1   MKLGISIFILLFLPLVLLAKADDGFVRTKGQQLILNGTPFYANGFNAYWLMYFASDPSLK 60

Query: 61  PKVSSAFQQAVNHGLSIGRTWAFNDGGYSPLQYSPGQYNEKMFQ---------------- 120
           PKVSSAFQQAV+HGLSIGRTWAFNDGGYSPLQYSPGQYNEKMFQ                
Sbjct: 61  PKVSSAFQQAVDHGLSIGRTWAFNDGGYSPLQYSPGQYNEKMFQGLDFVVAEARKYGIKL 120

Query: 121 ------------GKKQYVEWARSQGQAISSEDEFFTNPVVKGFYKNHIKSILTRVNSITG 180
                       GKKQYVEWARSQGQAISSEDEFFTNPVVKGFYKNHIKSILTRVNSITG
Sbjct: 121 ILSLVNNYESMGGKKQYVEWARSQGQAISSEDEFFTNPVVKGFYKNHIKSILTRVNSITG 180

Query: 181 VAYKDDPTIMAWELMNEARCPSDPSGNTIQAWIKEMASYLKSIDGKHLLEAGLEGFYGQS 240
           VAYKDDPTIMAWELMNEARCPSDPSGNTIQAWIKEMASYLKSIDGKHLLEAGLEGFYGQS
Sbjct: 181 VAYKDDPTIMAWELMNEARCPSDPSGNTIQAWIKEMASYLKSIDGKHLLEAGLEGFYGQS 240

Query: 241 KYQWNPNFQVGTDFIANNQIPEIDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQ 300
           KYQWNPNFQVGTDFIANNQIPEIDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQ
Sbjct: 241 KYQWNPNFQVGTDFIANNQIPEIDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQ 300

Query: 301 NILHKPVLFAEFGKSTKYSGADQRDQLFNAVYSAVYSSARGGGAAIGGMFWQLLVEGMDS 360
           NILHKPVLFAEFGKS KYSGADQRDQLFNAVYSAVYSSARGGGAAIGGMFWQLLVEGMDS
Sbjct: 301 NILHKPVLFAEFGKSIKYSGADQRDQLFNAVYSAVYSSARGGGAAIGGMFWQLLVEGMDS 360

Query: 361 FRDGYEVVLSESPSTANLITQESRRLIHIRRMYAKLRNIEKWKRAKEMKKAQWEASKGGN 398
           FRDGYEVVLSESPSTANLITQESRRLIHIRRMYAKLRNIEKWKRAKEMKKAQWEAS+GGN
Sbjct: 361 FRDGYEVVLSESPSTANLITQESRRLIHIRRMYAKLRNIEKWKRAKEMKKAQWEASRGGN 420

BLAST of PI0000809 vs. ExPASy TrEMBL
Match: A0A1S3CRH7 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103503926 PE=3 SV=1)

HSP 1 Score: 770.8 bits (1989), Expect = 2.8e-219
Identity = 386/425 (90.82%), Postives = 389/425 (91.53%), Query Frame = 0

Query: 1   MKLRIYFLILLLLPLVLLAKADDGFVRTKGQQLILNETPFYANGFNAYWLMYFASDPSQR 60
           MKL I   ILL LPLVLLAKADDGFVRTKGQQLILN TPFYANGFNAYWLMYFASDPS +
Sbjct: 1   MKLGISIFILLFLPLVLLAKADDGFVRTKGQQLILNGTPFYANGFNAYWLMYFASDPSLK 60

Query: 61  PKVSSAFQQAVNHGLSIGRTWAFNDGGYSPLQYSPGQYNEKMFQ---------------- 120
           PKVSSAFQQAV+HGLSIGRTWAFNDGGYSPLQYSPGQYNEKMFQ                
Sbjct: 61  PKVSSAFQQAVDHGLSIGRTWAFNDGGYSPLQYSPGQYNEKMFQGLDFVVAEARKYGIKL 120

Query: 121 ------------GKKQYVEWARSQGQAISSEDEFFTNPVVKGFYKNHIKSILTRVNSITG 180
                       GKKQYVEWARSQGQAISSEDEFFTNPVVKGFYKNHIKSILTRVNSITG
Sbjct: 121 ILSLVNNYESMGGKKQYVEWARSQGQAISSEDEFFTNPVVKGFYKNHIKSILTRVNSITG 180

Query: 181 VAYKDDPTIMAWELMNEARCPSDPSGNTIQAWIKEMASYLKSIDGKHLLEAGLEGFYGQS 240
           VAYKDDPTIMAWELMNEARCPSDPSGNTIQAWIKEMASYLKSIDGKHLLEAGLEGFYGQS
Sbjct: 181 VAYKDDPTIMAWELMNEARCPSDPSGNTIQAWIKEMASYLKSIDGKHLLEAGLEGFYGQS 240

Query: 241 KYQWNPNFQVGTDFIANNQIPEIDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQ 300
           KYQWNPNFQVGTDFIANNQIPEIDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQ
Sbjct: 241 KYQWNPNFQVGTDFIANNQIPEIDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQ 300

Query: 301 NILHKPVLFAEFGKSTKYSGADQRDQLFNAVYSAVYSSARGGGAAIGGMFWQLLVEGMDS 360
           NILHKPVLFAEFGKS KYSGADQRDQLFNAVYSAVYSSARGGGAAIGGMFWQLLVEGMDS
Sbjct: 301 NILHKPVLFAEFGKSIKYSGADQRDQLFNAVYSAVYSSARGGGAAIGGMFWQLLVEGMDS 360

Query: 361 FRDGYEVVLSESPSTANLITQESRRLIHIRRMYAKLRNIEKWKRAKEMKKAQWEASKGGN 398
           FRDGYEVVLSESPSTANLITQESRRLIHIRRMYAKLRNIEKWKRAKEMKKAQWEAS+GGN
Sbjct: 361 FRDGYEVVLSESPSTANLITQESRRLIHIRRMYAKLRNIEKWKRAKEMKKAQWEASRGGN 420

BLAST of PI0000809 vs. ExPASy TrEMBL
Match: A0A0A0LDZ5 (Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_3G859700 PE=3 SV=1)

HSP 1 Score: 758.1 bits (1956), Expect = 1.9e-215
Identity = 380/425 (89.41%), Postives = 385/425 (90.59%), Query Frame = 0

Query: 1   MKLRIYFLILLLLPLVLLAKADDGFVRTKGQQLILNETPFYANGFNAYWLMYFASDPSQR 60
           MKL I   ILLLL L+  AK D+GFV TKGQQLILN +PFYANGFNAYWLMYFASDPSQ 
Sbjct: 1   MKLGISIFILLLLLLLPRAKPDEGFVSTKGQQLILNGSPFYANGFNAYWLMYFASDPSQA 60

Query: 61  PKVSSAFQQAVNHGLSIGRTWAFNDGGYSPLQYSPGQYNEKMFQ---------------- 120
           PKVSSAFQQAVNHGLSIGRTWAFNDGGYSPLQYSPGQYNEKMFQ                
Sbjct: 61  PKVSSAFQQAVNHGLSIGRTWAFNDGGYSPLQYSPGQYNEKMFQGLDFVVAEARKYGIKL 120

Query: 121 ------------GKKQYVEWARSQGQAISSEDEFFTNPVVKGFYKNHIKSILTRVNSITG 180
                       GKKQYVEWARSQGQAISSEDEFFTNPVVKGFYKNHIKSILTRVNSITG
Sbjct: 121 ILSLVNNYASMGGKKQYVEWARSQGQAISSEDEFFTNPVVKGFYKNHIKSILTRVNSITG 180

Query: 181 VAYKDDPTIMAWELMNEARCPSDPSGNTIQAWIKEMASYLKSIDGKHLLEAGLEGFYGQS 240
           VAYKDDPTIMAWELMNEARCPSDPSGNTIQ WIKEMASYLKSIDGKHLLEAGLEGFYGQS
Sbjct: 181 VAYKDDPTIMAWELMNEARCPSDPSGNTIQGWIKEMASYLKSIDGKHLLEAGLEGFYGQS 240

Query: 241 KYQWNPNFQVGTDFIANNQIPEIDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQ 300
           KYQWNPNFQVGTDFIANNQIPEIDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQ
Sbjct: 241 KYQWNPNFQVGTDFIANNQIPEIDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQ 300

Query: 301 NILHKPVLFAEFGKSTKYSGADQRDQLFNAVYSAVYSSARGGGAAIGGMFWQLLVEGMDS 360
           NILHKPVLFAEFGKSTKYSGADQRDQLFNAVYSAVYSSARGGGAAIGGMFWQLLVEGMDS
Sbjct: 301 NILHKPVLFAEFGKSTKYSGADQRDQLFNAVYSAVYSSARGGGAAIGGMFWQLLVEGMDS 360

Query: 361 FRDGYEVVLSESPSTANLITQESRRLIHIRRMYAKLRNIEKWKRAKEMKKAQWEASKGGN 398
           FRDGYEVVLSESPSTANLITQESRRLIHIRRMYAKLRNIEKWKRAKEMK+AQWEAS+GGN
Sbjct: 361 FRDGYEVVLSESPSTANLITQESRRLIHIRRMYAKLRNIEKWKRAKEMKRAQWEASRGGN 420

BLAST of PI0000809 vs. ExPASy TrEMBL
Match: A0A6J1BS42 (Mannan endo-1,4-beta-mannosidase OS=Momordica charantia OX=3673 GN=LOC111005053 PE=3 SV=1)

HSP 1 Score: 694.1 bits (1790), Expect = 3.4e-196
Identity = 345/426 (80.99%), Postives = 378/426 (88.73%), Query Frame = 0

Query: 1   MKLRIYFL-ILLLLPLVLLAKADDGFVRTKGQQLILNETPFYANGFNAYWLMYFASDPSQ 60
           MKL   FL +LLL+ L   A+A+DGFVRT+GQQLILN +PFYANGFNAYWLMYFASDPSQ
Sbjct: 1   MKLISRFLVVLLLIVLRSEAEAEDGFVRTRGQQLILNGSPFYANGFNAYWLMYFASDPSQ 60

Query: 61  RPKVSSAFQQAVNHGLSIGRTWAFNDGGYSPLQYSPGQYNEKMFQ--------------- 120
           R KVSSAFQ+A+NHGLSIGRTWAF+DGGY+PLQYSPGQYNE+MFQ               
Sbjct: 61  RTKVSSAFQEALNHGLSIGRTWAFSDGGYNPLQYSPGQYNEQMFQGLDYVISEAGKHGIK 120

Query: 121 -------------GKKQYVEWARSQGQAISSEDEFFTNPVVKGFYKNHIKSILTRVNSIT 180
                        GKKQYVEWARSQGQAISSED+FFTN VVKGFYKNHIKS+L R NS+T
Sbjct: 121 LILSLVNNYENMGGKKQYVEWARSQGQAISSEDDFFTNSVVKGFYKNHIKSVLMRTNSLT 180

Query: 181 GVAYKDDPTIMAWELMNEARCPSDPSGNTIQAWIKEMASYLKSIDGKHLLEAGLEGFYGQ 240
           G+AYKDDPTIMAWELMNEARCPSDPSGNTIQAWI+EMASYLKSIDGKHLLEAGLEGFYGQ
Sbjct: 181 GIAYKDDPTIMAWELMNEARCPSDPSGNTIQAWIREMASYLKSIDGKHLLEAGLEGFYGQ 240

Query: 241 SKYQWNPNFQVGTDFIANNQIPEIDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDA 300
           S+ Q NPNFQVGT+FIANNQIPE+DFATVHSYPDQWLSGSSYENQL+FLNTWLNDHIQDA
Sbjct: 241 SRNQGNPNFQVGTNFIANNQIPELDFATVHSYPDQWLSGSSYENQLTFLNTWLNDHIQDA 300

Query: 301 QNILHKPVLFAEFGKSTKYSGADQRDQLFNAVYSAVYSSARGGGAAIGGMFWQLLVEGMD 360
           QNILHKPVLFAEFGKSTKYSG+DQRDQLFNAVYSAVYSSAR GGAA+GG+FWQLLVEGMD
Sbjct: 301 QNILHKPVLFAEFGKSTKYSGSDQRDQLFNAVYSAVYSSARTGGAAVGGLFWQLLVEGMD 360

Query: 361 SFRDGYEVVLSESPSTANLITQESRRLIHIRRMYAKLRNIEKWKRAKEMKKAQWEASKGG 398
           SFRDGYEVVL+E+ STANLI+QES++LIHIR+MYAKLRNIEK KRAKE+++AQW+A  GG
Sbjct: 361 SFRDGYEVVLNENLSTANLISQESQKLIHIRKMYAKLRNIEKLKRAKEIRRAQWQALNGG 420

BLAST of PI0000809 vs. NCBI nr
Match: KAA0041571.1 (mannan endo-1,4-beta-mannosidase 7-like [Cucumis melo var. makuwa] >TYK19685.1 mannan endo-1,4-beta-mannosidase 7-like [Cucumis melo var. makuwa])

HSP 1 Score: 779.2 bits (2011), Expect = 1.6e-221
Identity = 383/397 (96.47%), Postives = 387/397 (97.48%), Query Frame = 0

Query: 1   MKLRIYFLILLLLPLVLLAKADDGFVRTKGQQLILNETPFYANGFNAYWLMYFASDPSQR 60
           MKL I   ILL LPLVLLAKADDGFVRTKGQQLILN TPFYANGFNAYWLMYFASDPS +
Sbjct: 1   MKLGISIFILLFLPLVLLAKADDGFVRTKGQQLILNGTPFYANGFNAYWLMYFASDPSLK 60

Query: 61  PKVSSAFQQAVNHGLSIGRTWAFNDGGYSPLQYSPGQYNEKMFQGKKQYVEWARSQGQAI 120
           PKVSSAFQQAV+HGLSIGRTWAFNDGGYSPLQYSPGQYNEK+  GKKQYVEWARSQGQAI
Sbjct: 61  PKVSSAFQQAVDHGLSIGRTWAFNDGGYSPLQYSPGQYNEKIMGGKKQYVEWARSQGQAI 120

Query: 121 SSEDEFFTNPVVKGFYKNHIKSILTRVNSITGVAYKDDPTIMAWELMNEARCPSDPSGNT 180
           SSEDEFFTNPVVKGFYKNHIKSILTRVNSITGVAYKDDPTIMAWELMNEARCPSDPSGNT
Sbjct: 121 SSEDEFFTNPVVKGFYKNHIKSILTRVNSITGVAYKDDPTIMAWELMNEARCPSDPSGNT 180

Query: 181 IQAWIKEMASYLKSIDGKHLLEAGLEGFYGQSKYQWNPNFQVGTDFIANNQIPEIDFATV 240
           IQAWIKEMASYLKSIDGKHLLEAGLEGFYGQSKYQWNPNFQVGTDFIANNQIPEIDFATV
Sbjct: 181 IQAWIKEMASYLKSIDGKHLLEAGLEGFYGQSKYQWNPNFQVGTDFIANNQIPEIDFATV 240

Query: 241 HSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQNILHKPVLFAEFGKSTKYSGADQRDQLF 300
           HSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQNILHKPVLFAEFGKS KYSGADQRDQLF
Sbjct: 241 HSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQNILHKPVLFAEFGKSIKYSGADQRDQLF 300

Query: 301 NAVYSAVYSSARGGGAAIGGMFWQLLVEGMDSFRDGYEVVLSESPSTANLITQESRRLIH 360
           NAVYSAVYSSARGGGAAIGGMFWQLLVEGMDSFRDGYEVVLSESPSTANLITQESRRLIH
Sbjct: 301 NAVYSAVYSSARGGGAAIGGMFWQLLVEGMDSFRDGYEVVLSESPSTANLITQESRRLIH 360

Query: 361 IRRMYAKLRNIEKWKRAKEMKKAQWEASKGGNNSPGN 398
           IRRMYAKLRNIEKWKRAKEMKKAQWEAS+GGNNSPGN
Sbjct: 361 IRRMYAKLRNIEKWKRAKEMKKAQWEASRGGNNSPGN 397

BLAST of PI0000809 vs. NCBI nr
Match: XP_008466539.2 (PREDICTED: mannan endo-1,4-beta-mannosidase 7-like [Cucumis melo] >ADN34185.1 endo-beta-mannanase [Cucumis melo subsp. melo])

HSP 1 Score: 770.8 bits (1989), Expect = 5.8e-219
Identity = 386/425 (90.82%), Postives = 389/425 (91.53%), Query Frame = 0

Query: 1   MKLRIYFLILLLLPLVLLAKADDGFVRTKGQQLILNETPFYANGFNAYWLMYFASDPSQR 60
           MKL I   ILL LPLVLLAKADDGFVRTKGQQLILN TPFYANGFNAYWLMYFASDPS +
Sbjct: 1   MKLGISIFILLFLPLVLLAKADDGFVRTKGQQLILNGTPFYANGFNAYWLMYFASDPSLK 60

Query: 61  PKVSSAFQQAVNHGLSIGRTWAFNDGGYSPLQYSPGQYNEKMFQ---------------- 120
           PKVSSAFQQAV+HGLSIGRTWAFNDGGYSPLQYSPGQYNEKMFQ                
Sbjct: 61  PKVSSAFQQAVDHGLSIGRTWAFNDGGYSPLQYSPGQYNEKMFQGLDFVVAEARKYGIKL 120

Query: 121 ------------GKKQYVEWARSQGQAISSEDEFFTNPVVKGFYKNHIKSILTRVNSITG 180
                       GKKQYVEWARSQGQAISSEDEFFTNPVVKGFYKNHIKSILTRVNSITG
Sbjct: 121 ILSLVNNYESMGGKKQYVEWARSQGQAISSEDEFFTNPVVKGFYKNHIKSILTRVNSITG 180

Query: 181 VAYKDDPTIMAWELMNEARCPSDPSGNTIQAWIKEMASYLKSIDGKHLLEAGLEGFYGQS 240
           VAYKDDPTIMAWELMNEARCPSDPSGNTIQAWIKEMASYLKSIDGKHLLEAGLEGFYGQS
Sbjct: 181 VAYKDDPTIMAWELMNEARCPSDPSGNTIQAWIKEMASYLKSIDGKHLLEAGLEGFYGQS 240

Query: 241 KYQWNPNFQVGTDFIANNQIPEIDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQ 300
           KYQWNPNFQVGTDFIANNQIPEIDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQ
Sbjct: 241 KYQWNPNFQVGTDFIANNQIPEIDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQ 300

Query: 301 NILHKPVLFAEFGKSTKYSGADQRDQLFNAVYSAVYSSARGGGAAIGGMFWQLLVEGMDS 360
           NILHKPVLFAEFGKS KYSGADQRDQLFNAVYSAVYSSARGGGAAIGGMFWQLLVEGMDS
Sbjct: 301 NILHKPVLFAEFGKSIKYSGADQRDQLFNAVYSAVYSSARGGGAAIGGMFWQLLVEGMDS 360

Query: 361 FRDGYEVVLSESPSTANLITQESRRLIHIRRMYAKLRNIEKWKRAKEMKKAQWEASKGGN 398
           FRDGYEVVLSESPSTANLITQESRRLIHIRRMYAKLRNIEKWKRAKEMKKAQWEAS+GGN
Sbjct: 361 FRDGYEVVLSESPSTANLITQESRRLIHIRRMYAKLRNIEKWKRAKEMKKAQWEASRGGN 420

BLAST of PI0000809 vs. NCBI nr
Match: XP_004147857.3 (mannan endo-1,4-beta-mannosidase 7 [Cucumis sativus] >KGN60129.2 hypothetical protein Csa_001474 [Cucumis sativus])

HSP 1 Score: 760.4 bits (1962), Expect = 7.9e-216
Identity = 381/425 (89.65%), Postives = 386/425 (90.82%), Query Frame = 0

Query: 1   MKLRIYFLILLLLPLVLLAKADDGFVRTKGQQLILNETPFYANGFNAYWLMYFASDPSQR 60
           MKL I   ILLLL L+ LAK D+GFV TKGQQLILN +PFYANGFNAYWLMYFASDPSQ 
Sbjct: 1   MKLGISIFILLLLLLLPLAKPDEGFVSTKGQQLILNGSPFYANGFNAYWLMYFASDPSQA 60

Query: 61  PKVSSAFQQAVNHGLSIGRTWAFNDGGYSPLQYSPGQYNEKMFQ---------------- 120
           PKVSSAFQQAVNHGLSIGRTWAFNDGGYSPLQYSPGQYNEKMFQ                
Sbjct: 61  PKVSSAFQQAVNHGLSIGRTWAFNDGGYSPLQYSPGQYNEKMFQGLDFVVAEARKYGIKL 120

Query: 121 ------------GKKQYVEWARSQGQAISSEDEFFTNPVVKGFYKNHIKSILTRVNSITG 180
                       GKKQYVEWARSQGQAISSEDEFFTNPVVKGFYKNHIKSILTRVNSITG
Sbjct: 121 ILSLVNNYASMGGKKQYVEWARSQGQAISSEDEFFTNPVVKGFYKNHIKSILTRVNSITG 180

Query: 181 VAYKDDPTIMAWELMNEARCPSDPSGNTIQAWIKEMASYLKSIDGKHLLEAGLEGFYGQS 240
           VAYKDDPTIMAWELMNEARCPSDPSGNTIQ WIKEMASYLKSIDGKHLLEAGLEGFYGQS
Sbjct: 181 VAYKDDPTIMAWELMNEARCPSDPSGNTIQGWIKEMASYLKSIDGKHLLEAGLEGFYGQS 240

Query: 241 KYQWNPNFQVGTDFIANNQIPEIDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQ 300
           KYQWNPNFQVGTDFIANNQIPEIDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQ
Sbjct: 241 KYQWNPNFQVGTDFIANNQIPEIDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQ 300

Query: 301 NILHKPVLFAEFGKSTKYSGADQRDQLFNAVYSAVYSSARGGGAAIGGMFWQLLVEGMDS 360
           NILHKPVLFAEFGKSTKYSGADQRDQLFNAVYSAVYSSARGGGAAIGGMFWQLLVEGMDS
Sbjct: 301 NILHKPVLFAEFGKSTKYSGADQRDQLFNAVYSAVYSSARGGGAAIGGMFWQLLVEGMDS 360

Query: 361 FRDGYEVVLSESPSTANLITQESRRLIHIRRMYAKLRNIEKWKRAKEMKKAQWEASKGGN 398
           FRDGYEVVLSESPSTANLITQESRRLIHIRRMYAKLRNIEKWKRAKEMK+AQWEAS+GGN
Sbjct: 361 FRDGYEVVLSESPSTANLITQESRRLIHIRRMYAKLRNIEKWKRAKEMKRAQWEASRGGN 420

BLAST of PI0000809 vs. NCBI nr
Match: XP_038897716.1 (mannan endo-1,4-beta-mannosidase 7-like [Benincasa hispida])

HSP 1 Score: 742.7 bits (1916), Expect = 1.7e-210
Identity = 367/425 (86.35%), Postives = 387/425 (91.06%), Query Frame = 0

Query: 1   MKLRIYFLILLLLPLVLLAKADDGFVRTKGQQLILNETPFYANGFNAYWLMYFASDPSQR 60
           MKL I  L+ LLLPLVLLA+ADDGFVRT+GQQLILN TPFYANGFNAYWLMYFASDPSQR
Sbjct: 1   MKLSISILV-LLLPLVLLAEADDGFVRTRGQQLILNGTPFYANGFNAYWLMYFASDPSQR 60

Query: 61  PKVSSAFQQAVNHGLSIGRTWAFNDGGYSPLQYSPGQYNEKMFQ---------------- 120
            KVSSAFQQAVNHGLSIGRTWAF+DGGYSPLQYSPGQYNEKMFQ                
Sbjct: 61  TKVSSAFQQAVNHGLSIGRTWAFSDGGYSPLQYSPGQYNEKMFQGLDFVVSEARKYGIKL 120

Query: 121 ------------GKKQYVEWARSQGQAISSEDEFFTNPVVKGFYKNHIKSILTRVNSITG 180
                       GKKQYVEWARSQGQ+ISSED+FF+NPVVKGFYKNHIKS+LTR+NSITG
Sbjct: 121 ILSLVNNYESMGGKKQYVEWARSQGQSISSEDDFFSNPVVKGFYKNHIKSVLTRINSITG 180

Query: 181 VAYKDDPTIMAWELMNEARCPSDPSGNTIQAWIKEMASYLKSIDGKHLLEAGLEGFYGQS 240
           VAYKDDPTIMAWELMNEARCPSDPSGNTIQAWI+EMASYLKSIDGKHLLEAGLEGFYGQS
Sbjct: 181 VAYKDDPTIMAWELMNEARCPSDPSGNTIQAWIREMASYLKSIDGKHLLEAGLEGFYGQS 240

Query: 241 KYQWNPNFQVGTDFIANNQIPEIDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQ 300
           +YQWN NFQVGTDFIANNQIPE+DFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQ
Sbjct: 241 RYQWNTNFQVGTDFIANNQIPELDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQ 300

Query: 301 NILHKPVLFAEFGKSTKYSGADQRDQLFNAVYSAVYSSARGGGAAIGGMFWQLLVEGMDS 360
           NILHKPVL AEFGKSTKYSGADQRDQLFNAVYSAVYSSARGGGAA+GG+FWQLLVEGMDS
Sbjct: 301 NILHKPVLLAEFGKSTKYSGADQRDQLFNAVYSAVYSSARGGGAAVGGLFWQLLVEGMDS 360

Query: 361 FRDGYEVVLSESPSTANLITQESRRLIHIRRMYAKLRNIEKWKRAKEMKKAQWEASKGGN 398
           FRDGYEVVLSE+PSTANLI+QESRRLIHIR+MYAKLRNIEKWKRA+E++KAQWEA KGG 
Sbjct: 361 FRDGYEVVLSENPSTANLISQESRRLIHIRKMYAKLRNIEKWKRAREIRKAQWEALKGGK 420

BLAST of PI0000809 vs. NCBI nr
Match: XP_022132099.1 (mannan endo-1,4-beta-mannosidase 7-like [Momordica charantia])

HSP 1 Score: 694.1 bits (1790), Expect = 6.9e-196
Identity = 345/426 (80.99%), Postives = 378/426 (88.73%), Query Frame = 0

Query: 1   MKLRIYFL-ILLLLPLVLLAKADDGFVRTKGQQLILNETPFYANGFNAYWLMYFASDPSQ 60
           MKL   FL +LLL+ L   A+A+DGFVRT+GQQLILN +PFYANGFNAYWLMYFASDPSQ
Sbjct: 1   MKLISRFLVVLLLIVLRSEAEAEDGFVRTRGQQLILNGSPFYANGFNAYWLMYFASDPSQ 60

Query: 61  RPKVSSAFQQAVNHGLSIGRTWAFNDGGYSPLQYSPGQYNEKMFQ--------------- 120
           R KVSSAFQ+A+NHGLSIGRTWAF+DGGY+PLQYSPGQYNE+MFQ               
Sbjct: 61  RTKVSSAFQEALNHGLSIGRTWAFSDGGYNPLQYSPGQYNEQMFQGLDYVISEAGKHGIK 120

Query: 121 -------------GKKQYVEWARSQGQAISSEDEFFTNPVVKGFYKNHIKSILTRVNSIT 180
                        GKKQYVEWARSQGQAISSED+FFTN VVKGFYKNHIKS+L R NS+T
Sbjct: 121 LILSLVNNYENMGGKKQYVEWARSQGQAISSEDDFFTNSVVKGFYKNHIKSVLMRTNSLT 180

Query: 181 GVAYKDDPTIMAWELMNEARCPSDPSGNTIQAWIKEMASYLKSIDGKHLLEAGLEGFYGQ 240
           G+AYKDDPTIMAWELMNEARCPSDPSGNTIQAWI+EMASYLKSIDGKHLLEAGLEGFYGQ
Sbjct: 181 GIAYKDDPTIMAWELMNEARCPSDPSGNTIQAWIREMASYLKSIDGKHLLEAGLEGFYGQ 240

Query: 241 SKYQWNPNFQVGTDFIANNQIPEIDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDA 300
           S+ Q NPNFQVGT+FIANNQIPE+DFATVHSYPDQWLSGSSYENQL+FLNTWLNDHIQDA
Sbjct: 241 SRNQGNPNFQVGTNFIANNQIPELDFATVHSYPDQWLSGSSYENQLTFLNTWLNDHIQDA 300

Query: 301 QNILHKPVLFAEFGKSTKYSGADQRDQLFNAVYSAVYSSARGGGAAIGGMFWQLLVEGMD 360
           QNILHKPVLFAEFGKSTKYSG+DQRDQLFNAVYSAVYSSAR GGAA+GG+FWQLLVEGMD
Sbjct: 301 QNILHKPVLFAEFGKSTKYSGSDQRDQLFNAVYSAVYSSARTGGAAVGGLFWQLLVEGMD 360

Query: 361 SFRDGYEVVLSESPSTANLITQESRRLIHIRRMYAKLRNIEKWKRAKEMKKAQWEASKGG 398
           SFRDGYEVVL+E+ STANLI+QES++LIHIR+MYAKLRNIEK KRAKE+++AQW+A  GG
Sbjct: 361 SFRDGYEVVLNENLSTANLISQESQKLIHIRKMYAKLRNIEKLKRAKEIRRAQWQALNGG 420

BLAST of PI0000809 vs. TAIR 10
Match: AT5G66460.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 498.0 bits (1281), Expect = 6.8e-141
Identity = 241/388 (62.11%), Postives = 292/388 (75.26%), Query Frame = 0

Query: 24  GFVRTKGQQLILNETPFYANGFNAYWLMYFASDPSQRPKVSSAFQQAVNHGLSIGRTWAF 83
           GFVRTKG Q  LN  P+YANGFNAYWLMY ASDPSQR K+S+AFQ A  HGL++ RTWAF
Sbjct: 30  GFVRTKGVQFSLNGYPYYANGFNAYWLMYVASDPSQRSKISTAFQDASRHGLTVARTWAF 89

Query: 84  NDGGYSPLQYSPGQYNEKMFQ----------------------------GKKQYVEWARS 143
           +DGGY  LQYSPG YNE MFQ                            G+KQYV+WARS
Sbjct: 90  SDGGYRALQYSPGSYNEDMFQGLDFALAEARRHGIKIILSFANNYESFGGRKQYVDWARS 149

Query: 144 QGQAISSEDEFFTNPVVKGFYKNHIKSILTRVNSITGVAYKDDPTIMAWELMNEARCPSD 203
           +G+ +SSED+FFT+ +VK FYKNHIK++L R N+ T V YKDDPTIMAWELMNE RCPSD
Sbjct: 150 RGRPVSSEDDFFTDSLVKDFYKNHIKAVLNRFNTFTKVHYKDDPTIMAWELMNEPRCPSD 209

Query: 204 PSGNTIQAWIKEMASYLKSIDGKHLLEAGLEGFYGQSKYQ---WNPNFQVGTDFIANNQI 263
           PSG  IQAWI EMA+++KS+D  HLLEAGLEGFYGQS  Q    NP  Q GTDFIANN+I
Sbjct: 210 PSGRAIQAWITEMAAHVKSLDRNHLLEAGLEGFYGQSSPQSKTLNPPGQFGTDFIANNRI 269

Query: 264 PEIDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQNILHKPVLFAEFGKSTKYSG 323
           P IDF TVHSYPD+W   SS ++Q+ FLN WL+ HIQDAQN+LHKP++ AEFGKS K  G
Sbjct: 270 PGIDFVTVHSYPDEWFPDSSEQSQMDFLNKWLDAHIQDAQNVLHKPIILAEFGKSMKKPG 329

Query: 324 --ADQRDQLFNAVYSAVYSSARGGGAAIGGMFWQLLVEGMDSFRDGYEVVLSESPSTANL 379
               QRD +FN VYS +Y SA+ GGAA GG+FWQLLV G+D+F+DGY ++LS+S ST N+
Sbjct: 330 YTPAQRDIVFNTVYSKIYGSAKRGGAAAGGLFWQLLVNGIDNFQDGYGIILSQSSSTVNV 389

BLAST of PI0000809 vs. TAIR 10
Match: AT3G10890.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 385.6 bits (989), Expect = 4.9e-107
Identity = 193/404 (47.77%), Postives = 260/404 (64.36%), Query Frame = 0

Query: 1   MKLRIYFLILLLL------PLVLLAKADDGFVRTKGQQLILNETPFYANGFNAYWLMYFA 60
           MK   + ++L +L       L + + + DGFV  KG Q ILN  PFYANGFNAYWL Y A
Sbjct: 1   MKCLCFVVLLAILIAQNSSDLGVKSASSDGFVSRKGVQFILNGKPFYANGFNAYWLAYEA 60

Query: 61  SDPSQRPKVSSAFQQAVNHGLSIGRTWAFNDGGYSPLQYSPGQYNEKMFQ---------- 120
           +D + R K++  FQ A  H L+I RTW F DGGY  LQ +PG Y+EK FQ          
Sbjct: 61  TDSTTRFKITYVFQNATIHDLTIVRTWGFRDGGYRALQIAPGVYDEKTFQGLDFAIAEAK 120

Query: 121 ------------------GKKQYVEWARSQGQAISSEDEFFTNPVVKGFYKNHIKSILTR 180
                             G+KQYV+WA++ GQ +SS+D+F+TNP+VK +YKNH+K+++ R
Sbjct: 121 RLGIKMIITFVNNYSDFGGRKQYVDWAKNTGQNVSSDDDFYTNPLVKQYYKNHVKTMVNR 180

Query: 181 VNSITGVAYKDDPTIMAWELMNEARCPSDPSGNTIQAWIKEMASYLKSIDGKHLLEAGLE 240
           VN+ T V YKD+PTIM WELMNE +C +DPSG T+ AW+ EMA Y+KS+D KHLL  GLE
Sbjct: 181 VNTFTKVEYKDEPTIMGWELMNEPQCRADPSGKTLTAWMNEMALYVKSVDSKHLLSTGLE 240

Query: 241 GFYGQSKYQWNPNFQ------VGTDFIANNQIPEIDFATVHSYPDQWLSGSSYENQLSFL 300
           GFYG S  Q   +        +GTDFIAN+++  IDFA++HSYPD W      +++L+ L
Sbjct: 241 GFYGDSSPQRKTSLNPVAANVLGTDFIANHKLDAIDFASIHSYPDLWFPNLDEKSRLNLL 300

Query: 301 NTWLNDHIQDAQNILHKPVLFAEFGKSTKYSG--ADQRDQLFNAVYSAVYSSARGGGAAI 360
             WL  H++DAQNIL KP++  EFGK T   G    QRD +FNA +  +Y SA  GG A 
Sbjct: 301 RKWLECHLEDAQNILKKPLILGEFGKPTNTPGYTQAQRDAVFNATFDTIYESAEKGGPAA 360

Query: 361 GGMFWQLLVEGMDSFRDGYEVVLSESPSTANLITQESRRLIHIR 363
           G +FW ++ +GM++F+D   +VLSE+ +T N+IT+ESR+L  IR
Sbjct: 361 GALFWHVISDGMNNFKDPLSIVLSENSTTVNIITEESRKLGLIR 404

BLAST of PI0000809 vs. TAIR 10
Match: AT3G10900.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 354.8 bits (909), Expect = 9.3e-98
Identity = 183/395 (46.33%), Postives = 247/395 (62.53%), Query Frame = 0

Query: 7   FLILLLLPLV-----LLAKADDGFVRTKGQQLILNETPFYANGFNAYWLMYFASDPSQRP 66
           F++LL + +      + A   DGFV   G Q ILN  PFYANGFNAYWL Y A+DP+ R 
Sbjct: 6   FIVLLAIVIAQSYVGVEAAPSDGFVSRNGVQFILNGKPFYANGFNAYWLAYEATDPTTRF 65

Query: 67  KVSSAFQQAVNHGLSIGRTWAFNDGG-YSPLQYSPGQYNEKMFQ---------------- 126
           K+++ FQ A + GL+I RTW F DG  Y  LQ +PG Y+E+ FQ                
Sbjct: 66  KITNVFQNATSLGLTIARTWGFRDGAIYRALQTAPGSYDEQTFQGLDFVIAEAKRIGIKL 125

Query: 127 ------------GKKQYVEWARSQGQAISSEDEFFTNPVVKGFYKNHIKSILTRVNSITG 186
                       GKKQYV+WARS+G+ +SS D+F+ NPV+K FYKNH+K++L RVN+ T 
Sbjct: 126 IILLVNNWDDYGGKKQYVDWARSKGEVVSSNDDFYRNPVIKDFYKNHVKTVLNRVNTFTK 185

Query: 187 VAYKDDPTIMAWELMNEARCPSDPSGNTIQAWIKEMASYLKSIDGKHLLEAGLEGFYGQS 246
           VAYKD+P IMAW+LMNE RC  D SG T+  WI EMA ++KS+D  HLL  G EGFYG S
Sbjct: 186 VAYKDEPAIMAWQLMNEPRCGVDKSGKTLMDWINEMAPFVKSVDPNHLLSTGHEGFYGDS 245

Query: 247 ----KYQWNP--NFQVGTDFIANNQIPEIDFATVHSYPDQWLSGSSYENQLSFLNTWLND 306
               K   NP     VG DFIAN+ I  IDFA++H   D W       ++L+F+  WL  
Sbjct: 246 SPERKNSLNPVSANTVGADFIANHNIDAIDFASMHCGSDLWFQRLDQNSRLAFIKRWLEG 305

Query: 307 HIQDAQNILHKPVLFAEFG---KSTKYSGADQRDQLFNAVYSAVYSSARGGGAAIGGMFW 359
           HI+DAQNIL KPV+ AEFG    + +Y+ A+ RD +F   Y  +Y+SA+ GG+A G +FW
Sbjct: 306 HIEDAQNILKKPVILAEFGLGSDTPRYTLAN-RDGVFTTTYDIIYASAQKGGSAAGALFW 365

BLAST of PI0000809 vs. TAIR 10
Match: AT5G01930.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 350.9 bits (899), Expect = 1.3e-96
Identity = 170/369 (46.07%), Postives = 236/369 (63.96%), Query Frame = 0

Query: 26  VRTKGQQLILNETPFYANGFNAYWLMYFASDPSQRPKVSSAFQQAVNHGLSIGRTWAFND 85
           V+ KG Q  LN  PFY NGFN YW+M  A+D S R KV+  FQQA   G+++GRTWAFND
Sbjct: 48  VQRKGMQFTLNGQPFYVNGFNTYWMMTLAADNSTRGKVTEVFQQASAVGMTVGRTWAFND 107

Query: 86  GGYSPLQYSPGQYNEKMFQ----------------------------GKKQYVEWARSQG 145
           G +  LQ SP  Y+E++F+                            GK QYV+W  + G
Sbjct: 108 GQWRALQKSPSVYDEEVFKALDFVLSEARKYKIRLILSLVNNWDAYGGKAQYVKWGNASG 167

Query: 146 QAISSEDEFFTNPVVKGFYKNHIKSILTRVNSITGVAYKDDPTIMAWELMNEARCPSDPS 205
             ++S+D+FFTNP ++ FY++H++++L RVN+ T + YK+DPTI AWELMNE RCPSDPS
Sbjct: 168 LNLTSDDDFFTNPTLRNFYQSHVRTVLNRVNTFTNITYKNDPTIFAWELMNEPRCPSDPS 227

Query: 206 GNTIQAWIKEMASYLKSIDGKHLLEAGLEGFYGQS---KYQWNPN---FQVGTDFIANNQ 265
           G+ +Q+WI+EMA ++KS+D KHL+E GLEGFYG S   + ++NPN    QVGTDFI NNQ
Sbjct: 228 GDKLQSWIQEMAVFVKSLDAKHLVEIGLEGFYGPSAPARTRFNPNPYAAQVGTDFIRNNQ 287

Query: 266 IPEIDFATVHSYPDQWLSGSSYENQLSFLNTWLNDHIQDAQNILHKPVLFAEFGKSTKYS 325
           +  IDFA+VH YPD W+S +   + L F ++W+  H++DA+  L  PVLF EFG S    
Sbjct: 288 VLGIDFASVHVYPDSWISPAVSNSFLEFTSSWMQAHVEDAEMYLGMPVLFTEFGVSAHDP 347

Query: 326 GADQ--RDQLFNAVYSAVYSSARGGGAAIGGMFWQLLVEGMDSFRDGYEVVLSESPSTAN 359
           G +   RD + N VY    +S R GGA  G + WQ+  +G +   DGY V L+ + + + 
Sbjct: 348 GFNTSFRDMMLNTVYKMTLNSTRKGGAGAGSLVWQVFPQGAEFMDDGYAVYLTRAHTASK 407

BLAST of PI0000809 vs. TAIR 10
Match: AT1G02310.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 342.0 bits (876), Expect = 6.2e-94
Identity = 180/398 (45.23%), Postives = 240/398 (60.30%), Query Frame = 0

Query: 5   IYFLILLLLPLVLLAKADDGFVRTKGQQLILNETPFYANGFNAYWLMYFASDPSQ--RPK 64
           ++FL  L+    +      GFV   G Q +LN    Y NGFNAYW+M  A+D +   R  
Sbjct: 9   LFFLPFLIGNNRICVAVKTGFVGRNGTQFVLNGEQVYLNGFNAYWMMTTAADTASKGRAT 68

Query: 65  VSSAFQQAVNHGLSIGRTWAFNDGGYSPLQYSPGQYNEKMFQ------------------ 124
           V++A +QA   G+++ R W FN+G Y PLQ SPG Y+E +F+                  
Sbjct: 69  VTTALRQASAVGMNVARIWGFNEGDYIPLQISPGSYSEDVFKGLDFVVYEAGRFNIKLII 128

Query: 125 ----------GKKQYVEWARSQGQAISSEDEFFTNPVVKGFYKNHIKSILTRVNSITGVA 184
                     G+K+YVEWA      +   DEF+TN  VK FYKNH+K++LTR N+ITG  
Sbjct: 129 SLVNNFEDYGGRKKYVEWA-----GLDEPDEFYTNSAVKQFYKNHVKTVLTRKNTITGRM 188

Query: 185 YKDDPTIMAWELMNEARCPSDPSGNTIQAWIKEMASYLKSIDGKHLLEAGLEGFYGQS-- 244
           YKDDPTI +WEL+NE RC    + N +Q W+KEMASY+KSID  HLLE GLEGFYG+S  
Sbjct: 189 YKDDPTIFSWELINEPRCNDSTASNILQDWVKEMASYVKSIDSNHLLEIGLEGFYGESIP 248

Query: 245 -KYQWNPNFQV--GTDFIANNQIPEIDFATVHSYPDQW--LSGSSYENQLSFLNTWLNDH 304
            +  +NP  +V  GTDFI NNQIP+IDFAT+H YPD W  L  S    Q +F++ W+  H
Sbjct: 249 ERTVYNPGGRVLTGTDFITNNQIPDIDFATIHIYPDSWLPLQSSRTGEQDTFVDRWIGAH 308

Query: 305 IQDAQNILHKPVLFAEFGKSTKYSG--ADQRDQLFNAVYSAVYSSARGGGAAIGGMFWQL 363
           I+D  NI+ KP+L  EFGKS+KY G   ++R++ F  VY  +Y SAR GG+  GG+FWQL
Sbjct: 309 IEDCDNIIKKPLLITEFGKSSKYPGFSLEKRNKFFQRVYDVIYDSARAGGSCTGGVFWQL 368

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FJZ39.6e-14062.11Mannan endo-1,4-beta-mannosidase 7 OS=Arabidopsis thaliana OX=3702 GN=MAN7 PE=2 ... [more]
Q0JKM92.7e-12658.18Mannan endo-1,4-beta-mannosidase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=M... [more]
Q6Z3103.8e-11252.57Putative mannan endo-1,4-beta-mannosidase 9 OS=Oryza sativa subsp. japonica OX=3... [more]
Q8L5J16.7e-10951.67Mannan endo-1,4-beta-mannosidase 4 OS=Solanum lycopersicum OX=4081 GN=MAN4 PE=1 ... [more]
Q9SG946.9e-10647.77Mannan endo-1,4-beta-mannosidase 3 OS=Arabidopsis thaliana OX=3702 GN=MAN3 PE=2 ... [more]
Match NameE-valueIdentityDescription
A0A5A7TIV87.9e-22296.47Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
E5GCI62.8e-21990.82Mannan endo-1,4-beta-mannosidase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1[more]
A0A1S3CRH72.8e-21990.82Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103503926 PE=3 SV... [more]
A0A0A0LDZ51.9e-21589.41Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_3G859700 PE=3... [more]
A0A6J1BS423.4e-19680.99Mannan endo-1,4-beta-mannosidase OS=Momordica charantia OX=3673 GN=LOC111005053 ... [more]
Match NameE-valueIdentityDescription
KAA0041571.11.6e-22196.47mannan endo-1,4-beta-mannosidase 7-like [Cucumis melo var. makuwa] >TYK19685.1 m... [more]
XP_008466539.25.8e-21990.82PREDICTED: mannan endo-1,4-beta-mannosidase 7-like [Cucumis melo] >ADN34185.1 en... [more]
XP_004147857.37.9e-21689.65mannan endo-1,4-beta-mannosidase 7 [Cucumis sativus] >KGN60129.2 hypothetical pr... [more]
XP_038897716.11.7e-21086.35mannan endo-1,4-beta-mannosidase 7-like [Benincasa hispida][more]
XP_022132099.16.9e-19680.99mannan endo-1,4-beta-mannosidase 7-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT5G66460.16.8e-14162.11Glycosyl hydrolase superfamily protein [more]
AT3G10890.14.9e-10747.77Glycosyl hydrolase superfamily protein [more]
AT3G10900.19.3e-9846.33Glycosyl hydrolase superfamily protein [more]
AT5G01930.11.3e-9646.07Glycosyl hydrolase superfamily protein [more]
AT1G02310.16.2e-9445.23Glycosyl hydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001547Glycoside hydrolase, family 5PFAMPF00150Cellulasecoord: 154..302
e-value: 8.0E-8
score: 32.0
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 21..362
e-value: 2.7E-115
score: 387.3
NoneNo IPR availablePANTHERPTHR31451:SF43MANNAN ENDO-1,4-BETA-MANNOSIDASE-LIKE PROTEINcoord: 7..105
NoneNo IPR availablePANTHERPTHR31451:SF43MANNAN ENDO-1,4-BETA-MANNOSIDASE-LIKE PROTEINcoord: 103..382
IPR045053Mannan endo-1,4-beta-mannosidase-likePANTHERPTHR31451FAMILY NOT NAMEDcoord: 7..105
IPR045053Mannan endo-1,4-beta-mannosidase-likePANTHERPTHR31451FAMILY NOT NAMEDcoord: 103..382
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 25..350

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0000809.1PI0000809.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071704 organic substance metabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0016985 mannan endo-1,4-beta-mannosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds