PI0000770 (gene) Melon (PI 482460) v1

Overview


Name	PI0000770
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	E3 ubiquitin-protein ligase RHF1A
Location	chr11: 4846634 .. 4850376 (-)
RNA-Seq Expression	PI0000770
Synteny	PI0000770

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAGGGGAGTTTTTGAGAGCAGACTGATTCCACTTCCACCAAAAACCGTATGAATCGGTTTAGTTTGCTGCAAAGTTCTTCAGATTTTTCCTCCTCTCCCTATAAACTCTCTTCTTTGAGTTTGACCATTCTCTTTCTCTCTCTCTCTTTCTACACATTTCTAGGTTTCAGTTTTCCCCAATTCCCCTTCTTCTAAATTCCTTCCTTTCTTCTTCCCTTTGATTTTGTCTACACTTCATGGCCAATTTCACTTCTTCTTCTTCTTCTTCTTCTTTCTCTCTGAATCCCAATGCTGTTTCAGCTTCTGCTGCTGCTGCTTCTGATGATGATTTTGATGATGCTTGCTGTATTTGTCTCGACCCATTTACCTCCGATGATCCTGCCACTGTAAGTTCATCACTTAAACTTCCTCTTTCTTCACTACTGATTATAATTTTGTTCAATACTTGTAATGGGGTTTGCGTATTTTGACTGTTTGTGTGTTCTGAAGGAACATTCAGTTTCTTCTTTTTGGAGATCGTGTTTCCTTACCCCGTTCTGCATCTTGAAAGTGCAACAGGGTGTGTTTTTTTTTTTTTTCTTTTGAAGTGTTATGATCACCTGAGAATGAAAGGAGGAGACCCTTTTTTGTTTTTTTGGAGAAAATTGTGAAGAAGTGTACCTATTTGGTTTATGTTTTATTTTACGCAAATGGGTCTTGATTTTATTTGCTCGATCTTATTTGAATTATCGACGACTAAAAGTAGAATGTGATTCTCAATTCCTCATTGAGTGATTTATATATAATCTTCGATTGGAGATTTACAGGAGGATTTTCTACCCTTCGTATAACCAATGAAAAGCATCTCTTGCTAGACTTGGGCTAGCCCTTATTTTTACTCGAGCCAATTGGTTTCTTCACTAACATTCATTCATGTCTTTACAAACGGGTTTTGTGATTTCTCGTTTATTAGGATGAATTGATGAAGTGTTACTATAGAATGTAGGTGAGTAGATCTCTGATGATGGTTTTTCAACTAGTGTTGTGGGATTCCTTTTGTTTTCTGTCAAACTACTTTGTTTATATAACGACACAATATGACTTATTGTGCATGGTTTGAATTAATAACTTATTCGTCATAATTAGATGTTTAAATTTGATACAAAATCTGTAAGTAGATGAGTGAGACTGAGATTGGGTACAAGCGAATGATCATATTGCTGTTGTAGAAAATTGATCTGTTGAAGTGCTTTCTTCACTCCCTCTTGATAAAGGGATTTTGTCTCCAAGATGTTGATGGTCCATTCTAGGTGTTTTATTTGGCCTTTATGTTTGCATTAACTTTAACTATTGTTCAAATTTCAGATTACCAGTTGCAAGCATGAATATCATCTCCAATGTATTCTTGATTGGTAGGCTTCTTGAAGCTTTTTCTTTTTTATCCGATCTTCTAAAAGCAGGCTGTAATGGAAGTCTTACCTTTTGGGCATTGATTTTTCAGGTCTCAAAGAAGCGATGAGTGCCCAATCTGTTGCCAGTTGCTAGTGTTGAAGGATCCTGTTGGGTATGTAACTTTGTCATAGAAAGTTTAATTCCTTTCACATTGTCCTCACAATTCTTGTGCCTCGCTCTATCTCTCTTAATCAATCTACTTAATGAAAATGACACAATCTTTCTAATCAAATGTGTGCTAATTCAACTATTCTACAGTCAGGAGCTTCTAGCAGCTATGGCTAATGAAAGGCTCTTGAAGTCGAGAAGCTTGTCTTCTGCTGCATCTACTTCTTTGCGTTTCCACGAAAACTTTGATATTGATCACGTGAGTAGTTCTAATAAATATGGCAATCTATCTTTCTTATCTTTCTTCCTTATGCTTAATTGTTGCTTCAACAAAGATCGACCATTATTAAGCAGCAAAATTGATTCCTTCAGGACTCTGCGCACTCGGATGACTCCGACTTTGATGACCTGATTATGCAGCATCTTGCTGCTGCAGCTAGCCGAGCTCGATATGTTCATAGAAGGGAGAGGCAAAGACATTTTGGACTTGGGCCTTCACAAGTTGCCTCTGCATGTCCAGAGGTTCCATTGTCACCACGATTTCAAGATGCAACTCTAACTTCACCTAATAGTGATTCACCATCTCCCGGTTCTCCAATACCATCGGTTGGCCGAACTGGAATTATTAAAATCTCCCCTAGGTACATTACATCCCTGGACTAACATATCTACTACATGCTTTGCTCTTTCTTAATACCAACCATACTTGACTTAAATTTAAGTTGGTTTCTTGTAAAAGAGAGTGCCTTCTAGTACTCTATAGAGAAATTCTTTCTTCTCTTGATTGCAACCTTAAATTCTCAAGACGCCCTCAAGCTTCAAAACAAGCATACCTTTCGTTTGTTTTACCATCTAACATGCATCAACCTTTTTGTTGTATAGTGTAATTTTACTGCCCGATACACCGTCTGGTAGTCAACAGAAAACAAACCAATCTGAGGGGCTCTCTTTCCCAGATTCCATCAAATCCAAATGGTCTGCTACTTCTGCAAAGTAAGTGTAATGGAGTTTGGTTCTGTTAAATTAGTTTCTTTCTACATGTCACATCATCAATTCTAAATTGCATACCAAAATTTAAAAACCTGTACCAATACAGGTACAAGGAGTCAATCTTGCGAAGCACTCAAGGTATTAAGGAGAAACTGCTTGCTCGTAACAGCTCCGTCAAGGAGCTAAGCAAGGGAGTAAAGCGTGAAGTTAGTGCTGGGATTGCTGGCGTTGCTCGAATGATCGATCGCCTAGATTTGACTTCAAAAAGGAATACTGGTTCTGTTTCCTTCTTCAGCTGTGGTGGGGGTACATCAAACTCCTTGAAAGGAAAGAACGTAGTGCAAGAGAGTGCTGTCACTGATGGTTCAGTTAAAATTGATAGAATATGTACTGGTTCACCTTCACAAGTTTCCCCAACCGTTCCAGGCTCCGTCGAAGTTTCTTTAGCTCAGGTACTTCACTTTATGTCGTTCAACGATTTTCCACAAACTAGTGTCAAACTATACCTTACTTTGTAAAAGACTTTTGCTTAAATCATTCCACAATTTATGTGTTTAATGTCTTTATTGATTATCATTCATCTGTTTGGCAGAGAGGAGATTGAAGTTTGGATGTTAGGATTGGACTCATCTAGTTGAACATCTTATCAACTCAACTCAACAGAATGCACACTGCGGCAAGCGACGTGTTACGACTTAGCCAACATCCTTGTTGGTTTGCGTCCTGCATTTTCTTCTTTCTATAAATCTACTAATCAAAAAGGTGTAATTCTCTTTGTAATTCAATATATATATTTTTTTATGTAATGATGATCTGCTTCCATGATACACAATTTCTATGTGGGCGAGTTTCTTAAAACTCATTGGGATAAATGCTTCTTTTGAAGGGGGAAGAGCATAACAAAGGATGATGGTTTCTATTCTGAATTCTCTTGAAGCCAACCTAAGTGCAATGTCAAGGGGAATATTAGGTTAGGGTGGAAACAAAATACCAAAAACTGCATTTTTTAAGCCAAGTCAATTCCCATGCCAATTTTGTAACTTGAAAATTGAAATTGATCTCATGGTCTGAGAGTCTTTGGAAAAAAAAAAAGTTGTGCAGTATACATTAGAAATTTTCATATCCAGCGAAATTGGCATTCTATATTGATATGTCTATGTCGAAATCAACCTTCTAAATTTTATTGATTGTTCTAATAATACACAACTTTTG

mRNA sequence

AAAAGGGGAGTTTTTGAGAGCAGACTGATTCCACTTCCACCAAAAACCGTATGAATCGGTTTAGTTTGCTGCAAAGTTCTTCAGATTTTTCCTCCTCTCCCTATAAACTCTCTTCTTTGAGTTTGACCATTCTCTTTCTCTCTCTCTCTTTCTACACATTTCTAGGTTTCAGTTTTCCCCAATTCCCCTTCTTCTAAATTCCTTCCTTTCTTCTTCCCTTTGATTTTGTCTACACTTCATGGCCAATTTCACTTCTTCTTCTTCTTCTTCTTCTTTCTCTCTGAATCCCAATGCTGTTTCAGCTTCTGCTGCTGCTGCTTCTGATGATGATTTTGATGATGCTTGCTGTATTTGTCTCGACCCATTTACCTCCGATGATCCTGCCACTATTACCAGTTGCAAGCATGAATATCATCTCCAATGTATTCTTGATTGGTCTCAAAGAAGCGATGAGTGCCCAATCTGTTGCCAGTTGCTAGTGTTGAAGGATCCTGTTGGTCAGGAGCTTCTAGCAGCTATGGCTAATGAAAGGCTCTTGAAGTCGAGAAGCTTGTCTTCTGCTGCATCTACTTCTTTGCGTTTCCACGAAAACTTTGATATTGATCACGACTCTGCGCACTCGGATGACTCCGACTTTGATGACCTGATTATGCAGCATCTTGCTGCTGCAGCTAGCCGAGCTCGATATGTTCATAGAAGGGAGAGGCAAAGACATTTTGGACTTGGGCCTTCACAAGTTGCCTCTGCATGTCCAGAGGTTCCATTGTCACCACGATTTCAAGATGCAACTCTAACTTCACCTAATAGTGATTCACCATCTCCCGGTTCTCCAATACCATCGGTTGGCCGAACTGGAATTATTAAAATCTCCCCTAGTGTAATTTTACTGCCCGATACACCGTCTGGTAGTCAACAGAAAACAAACCAATCTGAGGGGCTCTCTTTCCCAGATTCCATCAAATCCAAATGGTCTGCTACTTCTGCAAAGTACAAGGAGTCAATCTTGCGAAGCACTCAAGGTATTAAGGAGAAACTGCTTGCTCGTAACAGCTCCGTCAAGGAGCTAAGCAAGGGAGTAAAGCGTGAAGTTAGTGCTGGGATTGCTGGCGTTGCTCGAATGATCGATCGCCTAGATTTGACTTCAAAAAGGAATACTGGTTCTGTTTCCTTCTTCAGCTGTGGTGGGGGTACATCAAACTCCTTGAAAGGAAAGAACGTAGTGCAAGAGAGTGCTGTCACTGATGGTTCAGTTAAAATTGATAGAATATGTACTGGTTCACCTTCACAAGTTTCCCCAACCGTTCCAGGCTCCGTCGAAGTTTCTTTAGCTCAGAGAGGAGATTGAAGTTTGGATGTTAGGATTGGACTCATCTAGTTGAACATCTTATCAACTCAACTCAACAGAATGCACACTGCGGCAAGCGACGTGTTACGACTTAGCCAACATCCTTGTTGGTTTGCGTCCTGCATTTTCTTCTTTCTATAAATCTACTAATCAAAAAGGTGTAATTCTCTTTGTAATTCAATATATATATTTTTTTATGTAATGATGATCTGCTTCCATGATACACAATTTCTATGTGGGCGAGTTTCTTAAAACTCATTGGGATAAATGCTTCTTTTGAAGGGGGAAGAGCATAACAAAGGATGATGGTTTCTATTCTGAATTCTCTTGAAGCCAACCTAAGTGCAATGTCAAGGGGAATATTAGGTTAGGGTGGAAACAAAATACCAAAAACTGCATTTTTTAAGCCAAGTCAATTCCCATGCCAATTTTGTAACTTGAAAATTGAAATTGATCTCATGGTCTGAGAGTCTTTGGAAAAAAAAAAAGTTGTGCAGTATACATTAGAAATTTTCATATCCAGCGAAATTGGCATTCTATATTGATATGTCTATGTCGAAATCAACCTTCTAAATTTTATTGATTGTTCTAATAATACACAACTTTTG

Coding sequence (CDS)

ATGGCCAATTTCACTTCTTCTTCTTCTTCTTCTTCTTTCTCTCTGAATCCCAATGCTGTTTCAGCTTCTGCTGCTGCTGCTTCTGATGATGATTTTGATGATGCTTGCTGTATTTGTCTCGACCCATTTACCTCCGATGATCCTGCCACTATTACCAGTTGCAAGCATGAATATCATCTCCAATGTATTCTTGATTGGTCTCAAAGAAGCGATGAGTGCCCAATCTGTTGCCAGTTGCTAGTGTTGAAGGATCCTGTTGGTCAGGAGCTTCTAGCAGCTATGGCTAATGAAAGGCTCTTGAAGTCGAGAAGCTTGTCTTCTGCTGCATCTACTTCTTTGCGTTTCCACGAAAACTTTGATATTGATCACGACTCTGCGCACTCGGATGACTCCGACTTTGATGACCTGATTATGCAGCATCTTGCTGCTGCAGCTAGCCGAGCTCGATATGTTCATAGAAGGGAGAGGCAAAGACATTTTGGACTTGGGCCTTCACAAGTTGCCTCTGCATGTCCAGAGGTTCCATTGTCACCACGATTTCAAGATGCAACTCTAACTTCACCTAATAGTGATTCACCATCTCCCGGTTCTCCAATACCATCGGTTGGCCGAACTGGAATTATTAAAATCTCCCCTAGTGTAATTTTACTGCCCGATACACCGTCTGGTAGTCAACAGAAAACAAACCAATCTGAGGGGCTCTCTTTCCCAGATTCCATCAAATCCAAATGGTCTGCTACTTCTGCAAAGTACAAGGAGTCAATCTTGCGAAGCACTCAAGGTATTAAGGAGAAACTGCTTGCTCGTAACAGCTCCGTCAAGGAGCTAAGCAAGGGAGTAAAGCGTGAAGTTAGTGCTGGGATTGCTGGCGTTGCTCGAATGATCGATCGCCTAGATTTGACTTCAAAAAGGAATACTGGTTCTGTTTCCTTCTTCAGCTGTGGTGGGGGTACATCAAACTCCTTGAAAGGAAAGAACGTAGTGCAAGAGAGTGCTGTCACTGATGGTTCAGTTAAAATTGATAGAATATGTACTGGTTCACCTTCACAAGTTTCCCCAACCGTTCCAGGCTCCGTCGAAGTTTCTTTAGCTCAGAGAGGAGATTGA

Protein sequence

MANFTSSSSSSSFSLNPNAVSASAAAASDDDFDDACCICLDPFTSDDPATITSCKHEYHLQCILDWSQRSDECPICCQLLVLKDPVGQELLAAMANERLLKSRSLSSAASTSLRFHENFDIDHDSAHSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFGLGPSQVASACPEVPLSPRFQDATLTSPNSDSPSPGSPIPSVGRTGIIKISPSVILLPDTPSGSQQKTNQSEGLSFPDSIKSKWSATSAKYKESILRSTQGIKEKLLARNSSVKELSKGVKREVSAGIAGVARMIDRLDLTSKRNTGSVSFFSCGGGTSNSLKGKNVVQESAVTDGSVKIDRICTGSPSQVSPTVPGSVEVSLAQRGD

Homology

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BLAST of PI0000770 vs. ExPASy Swiss-Prot
Match: Q4TU14 (E3 ubiquitin-protein ligase RHF1A OS=Arabidopsis thaliana OX=3702 GN=RHF1A PE=1 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 3.3e-62
Identity = 168/368 (45.65%), Postives = 223/368 (60.60%), Query Frame = 0


Query: 1   MANFTSSSS---SSSFSLNPNAVSASAAA-----ASDDD--FDDACCICLDPFTSDDPAT 60
           M+NFT +S+   S +   NP   S+S+++     ASDDD   DDAC ICL+PFT  DP+T
Sbjct: 1   MSNFTYTSAFNLSDNSPFNPAIGSSSSSSSALVVASDDDNNTDDACSICLEPFTLQDPST 60

Query: 61  ITSCKHEYHLQCILDWSQRSDECPICCQLLVLKDPVGQELLAAMANERLLKSRSLSSAAS 120
           +TSCKHEYHLQCI++WSQRS ECPIC QL VL+DP  QELLAA+  ERLLK+R++SS++ 
Sbjct: 61  VTSCKHEYHLQCIIEWSQRSKECPICWQLFVLRDPASQELLAAVEKERLLKTRNISSSSP 120

Query: 121 TSLRFHENFDIDHDSAHSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFGLGPSQVASA 180
            S+  H + D   +   S  S FD+  ++HL  AA R   + RR+ Q    L  S   + 
Sbjct: 121 ISIH-HSHDDFHSEEEESQFSSFDEQFLRHLTEAAHRRCLLRRRDGQISSSLVSSSDPTT 180

Query: 181 CPEVPLSPRFQDATLT-----------SPNSDSPSP---GSPIPSVGRTGIIKISPSVIL 240
                L   ++ + ++           SP S +PSP    S IP+    G  +ISP    
Sbjct: 181 IHPTDLVNLYRLSAISHVEHQNSNPCPSPGSMTPSPVSGHSSIPADSNNG-SRISPG--- 240

Query: 241 LPDTPSGSQQKTNQSEGLSFPDSIKSKWSATSAKYKESILRSTQGIKEKLLARNSSVKEL 300
              +PS S Q     E  S P++IKSK +A SAKYKESI +S QG+KEKLLARN+SVKEL
Sbjct: 241 --PSPSRSSQSPKSPEASSLPEAIKSKLAAASAKYKESISKSKQGLKEKLLARNNSVKEL 300

Query: 301 SKGVKREVSAGIAGVARMIDRLDLTSKRNTGSV---SFFSCGGGTSNSLKGKNVVQESAV 342
           SKGV+RE++AGIAGVARMI+R+D +SKR  GS    +  +   G + S KGK V   S  
Sbjct: 301 SKGVQREMNAGIAGVARMIERMDFSSKRFGGSAHVSTSTATASGFNFSFKGKRVEANSKS 360

[Close]

BLAST of PI0000770 vs. ExPASy Swiss-Prot
Match: Q9ZT42 (E3 ubiquitin-protein ligase RHF2A OS=Arabidopsis thaliana OX=3702 GN=RHF2A PE=2 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 6.1e-40
Identity = 137/368 (37.23%), Postives = 196/368 (53.26%), Query Frame = 0


Query: 19  AVSASAAAASDDDFDDACCICLDPFTSDDPATITSCKHEYHLQCILDWSQRSDECPICCQ 78
           + +A       D  DDAC ICL+ F   DP+T+TSCKHEYHLQCIL+W QRS +CP+C Q
Sbjct: 16  SAAAFVEGGIQDACDDACSICLESFCESDPSTLTSCKHEYHLQCILEWCQRSSQCPMCWQ 75

Query: 79  LLVLKDPVGQELLAAMANERLLKSRSLSSAASTSLRFHENFDIDHDSAHSDDSDFDDLIM 138
            + LKDP  QELL A+  ER  +     +A         +F++ H     D+++ ++ I+
Sbjct: 76  SISLKDPTSQELLEAVEQERNFRFNPTRNATIFRHPTLGDFELQHLPVGVDNAEIEERII 135

Query: 139 QHLAAAAS--RARYVHRRERQR---------HFGLGPSQVASAC----PEVPLSPRFQDA 198
           QHLAAAA+  RAR+  RRE  R          F +  SQ  ++     P +P SP  +D 
Sbjct: 136 QHLAAAAAMGRARHGVRREGHRSRSSSQGHQQFMVFSSQPNASSPPPHPPMPSSPSQRDE 195

Query: 199 TLTSPN------------SDSPSPGSPIPSVGRTGIIKISPSVI------LLPDTPSGSQ 258
           + T  N            S++  P S  P        ++SPS        L   +PS  Q
Sbjct: 196 SDTVSNLPHNALGEGSHQSNTQPPTSSHPR-------QVSPSASDSNSRPLNQSSPS-EQ 255

Query: 259 QKTNQSEGLSFPDSIKSKWSATSAKYKESILRSTQGIKEKLLARNSSVKELSKGVKREVS 318
            +   SE  SF +S+KS+ +A S +YKESI ++T+  K++L +RN+S+ +L   VKREVS
Sbjct: 256 DRAGPSELQSFSESLKSRLNAVSTRYKESISKNTRNWKDRLFSRNTSMADLGSEVKREVS 315

Query: 319 AGIAGVARMIDRLDL--TSKRNTGSVSFFSCG--GGTSNSLKGKNVVQESAVTDGSVKID 350
           AGIA V+RM++RL+    S+ +T SVS  S      T+N         E +V +  VK +
Sbjct: 316 AGIATVSRMMERLETRENSRPSTASVSDVSENHTPETNNEHNRAAAGDEHSVNERGVK-E 374

[Close]

BLAST of PI0000770 vs. ExPASy Swiss-Prot
Match: Q2HIJ8 (Probable E3 ubiquitin-protein ligase RHB1A OS=Arabidopsis thaliana OX=3702 GN=RHB1A PE=2 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 5.6e-09
Identity = 24/55 (43.64%), Postives = 37/55 (67.27%), Query Frame = 0


Query: 30  DDFDDACCICLDPFTSDDPATITSCKHEYHLQCILDWSQRSDECPICCQLLVLKD 85
           D+ +D C IC + +  ++P   T C+HE+HL C+L+W +RSD CPIC + +V  D
Sbjct: 133 DEEEDCCPICFEDYDVENPRLTTKCEHEFHLSCLLEWIERSDRCPICDKEVVFDD 187

[Close]

BLAST of PI0000770 vs. ExPASy Swiss-Prot
Match: Q93ZF6 (E3 ubiquitin-protein ligase AIRP1 OS=Arabidopsis thaliana OX=3702 GN=AIRP1 PE=1 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 2.1e-08
Identity = 24/51 (47.06%), Postives = 35/51 (68.63%), Query Frame = 0


Query: 34  DACCICLDPFTSDDPATITSCKHEYHLQCILDWSQRSDECPICCQLLVLKD 85
           D C ICL+ +  D+P  +T C H++HL CIL W +RS+ CP+C + LVL +
Sbjct: 102 DECPICLEEYEIDNPKLLTKCGHDFHLACILAWMERSEACPVCDKELVLTE 152

[Close]

BLAST of PI0000770 vs. ExPASy Swiss-Prot
Match: Q8LE94 (E3 ubiquitin-protein ligase At3g02290 OS=Arabidopsis thaliana OX=3702 GN=At3g02290 PE=2 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 2.1e-08
Identity = 20/48 (41.67%), Postives = 34/48 (70.83%), Query Frame = 0


Query: 33  DDACCICLDPFTSDDPATITSCKHEYHLQCILDWSQRSDECPICCQLL 81
           +D C  CL+ +TS++P  +T C H +HL CI +W +RS+ CP+C +++
Sbjct: 178 EDVCPTCLEEYTSENPKIVTKCSHHFHLSCIYEWMERSENCPVCGKVM 225

[Close]

BLAST of PI0000770 vs. ExPASy TrEMBL
Match: A0A1S3C9D8 (E3 ubiquitin-protein ligase RHF1A OS=Cucumis melo OX=3656 GN=LOC103497932 PE=4 SV=1)

HSP 1 Score: 656.4 bits (1692), Expect = 7.2e-185
Identity = 353/371 (95.15%), Postives = 359/371 (96.77%), Query Frame = 0


Query: 1   MANFTSSS--SSSSFSLNPNAVSAS-AAAASDDDFDDACCICLDPFTSDDPATITSCKHE 60
           MANFTSSS  S SS SLNPNAVSAS AAAASDDDFDDACCICLDPFTSDDPATITSCKHE
Sbjct: 1   MANFTSSSSPSPSSVSLNPNAVSASAAAAASDDDFDDACCICLDPFTSDDPATITSCKHE 60

Query: 61  YHLQCILDWSQRSDECPICCQLLVLKDPVGQELLAAMANERLLKSRSLSSAASTSLRFHE 120
           YHLQCILDWSQRSDECPICCQLL LKDPVGQELLAAMA+ERLLKSRSLSSAASTSLRFHE
Sbjct: 61  YHLQCILDWSQRSDECPICCQLLALKDPVGQELLAAMASERLLKSRSLSSAASTSLRFHE 120

Query: 121 NFDIDHDSAHSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFGLGPSQVASACPEVPLS 180
           NFD DHDS HSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFG+GPSQVASACPEVP+S
Sbjct: 121 NFDFDHDSVHSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFGVGPSQVASACPEVPMS 180

Query: 181 PRFQDATLTSPNSDSPSPGSPIPSVGRTGIIKISPSVILLPDTPSGSQQKTNQSEGLSFP 240
           PRFQDATLTSPNSDSPSPGSP+PSVG+TGI KISPSVILLPDTPSGSQQKTNQSEGLSF 
Sbjct: 181 PRFQDATLTSPNSDSPSPGSPMPSVGQTGINKISPSVILLPDTPSGSQQKTNQSEGLSFQ 240

Query: 241 DSIKSKWSATSAKYKESILRSTQGIKEKLLARNSSVKELSKGVKREVSAGIAGVARMIDR 300
           DSIKSKWSATSAKYKESILRSTQGIKEKLLARNSSVKELSKGVKREVSAGIAGVARMIDR
Sbjct: 241 DSIKSKWSATSAKYKESILRSTQGIKEKLLARNSSVKELSKGVKREVSAGIAGVARMIDR 300

Query: 301 LDLTSKRNTGSVSFFSCGGGTSNSLKGKNVVQESAVTDGSVKIDRICTGSPSQVSPTVPG 360
           LDLTSKRNTGSVSFFSC GGTSNSLKGKNVVQESA TDGSVKI+RICTGSPSQVSPTVPG
Sbjct: 301 LDLTSKRNTGSVSFFSC-GGTSNSLKGKNVVQESAGTDGSVKINRICTGSPSQVSPTVPG 360

Query: 361 SVEVSLAQRGD 369
           SVEVSLAQRGD
Sbjct: 361 SVEVSLAQRGD 370

[Close]

BLAST of PI0000770 vs. ExPASy TrEMBL
Match: A0A0A0KDQ6 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G153470 PE=4 SV=1)

HSP 1 Score: 647.9 bits (1670), Expect = 2.6e-182
Identity = 345/368 (93.75%), Postives = 352/368 (95.65%), Query Frame = 0


Query: 1   MANFTSSSSSSSFSLNPNAVSASAAAASDDDFDDACCICLDPFTSDDPATITSCKHEYHL 60
           MANFT  SSSS  SLNPNAVSAS AAASDDDFDD CCICLDPFTSDDPATITSCKHEYHL
Sbjct: 1   MANFT--SSSSFVSLNPNAVSAS-AAASDDDFDDVCCICLDPFTSDDPATITSCKHEYHL 60

Query: 61  QCILDWSQRSDECPICCQLLVLKDPVGQELLAAMANERLLKSRSLSSAASTSLRFHENFD 120
           QCILDWSQRSDECPICCQLLVLKDPVGQELLAAMA+ERLLKSR LSSAASTSL FHENFD
Sbjct: 61  QCILDWSQRSDECPICCQLLVLKDPVGQELLAAMASERLLKSRGLSSAASTSLHFHENFD 120

Query: 121 IDHDSAHSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFGLGPSQVASACPEVPLSPRF 180
            DHDS HSDDSDFDDLIMQHLAAAASRARYV RRERQRHFG+GPSQV+SACPEVP+SPRF
Sbjct: 121 FDHDSVHSDDSDFDDLIMQHLAAAASRARYVQRRERQRHFGVGPSQVSSACPEVPMSPRF 180

Query: 181 QDATLTSPNSDSPSPGSPIPSVGRTGIIKISPSVILLPDTPSGSQQKTNQSEGLSFPDSI 240
           QDATLTSPNSDSPSP SP+PSVGRTGI KISPSVILLPDTPSGSQQKTNQSEGLSF DSI
Sbjct: 181 QDATLTSPNSDSPSPSSPMPSVGRTGINKISPSVILLPDTPSGSQQKTNQSEGLSFQDSI 240

Query: 241 KSKWSATSAKYKESILRSTQGIKEKLLARNSSVKELSKGVKREVSAGIAGVARMIDRLDL 300
           KSKWSATSAKYKESILRSTQGIKEKLLARNSSVKELSKGVKREVSAGIAGVARMIDRLDL
Sbjct: 241 KSKWSATSAKYKESILRSTQGIKEKLLARNSSVKELSKGVKREVSAGIAGVARMIDRLDL 300

Query: 301 TSKRNTGSVSFFSCGGGTSNSLKGKNVVQESAVTDGSVKIDRICTGSPSQVSPTVPGSVE 360
           TSKRNTGSVSFFSC GGTSNSLKGKNVVQESAVTDGSVKI+RICTGSPSQVSPTVP SV+
Sbjct: 301 TSKRNTGSVSFFSCSGGTSNSLKGKNVVQESAVTDGSVKINRICTGSPSQVSPTVPSSVK 360

Query: 361 VSLAQRGD 369
           VSLAQRGD
Sbjct: 361 VSLAQRGD 365

[Close]

BLAST of PI0000770 vs. ExPASy TrEMBL
Match: A0A5A7SVT0 (E3 ubiquitin-protein ligase RHF1A OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold111G00690 PE=4 SV=1)

HSP 1 Score: 582.8 bits (1501), Expect = 1.0e-162
Identity = 305/318 (95.91%), Postives = 311/318 (97.80%), Query Frame = 0


Query: 51  ITSCKHEYHLQCILDWSQRSDECPICCQLLVLKDPVGQELLAAMANERLLKSRSLSSAAS 110
           ITSCKHEYHLQCILDWSQRSDECPICCQLL LKDPVGQELLAAMA+ERLLKSRSLSSAAS
Sbjct: 4   ITSCKHEYHLQCILDWSQRSDECPICCQLLALKDPVGQELLAAMASERLLKSRSLSSAAS 63

Query: 111 TSLRFHENFDIDHDSAHSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFGLGPSQVASA 170
           TSLRFHENFD DHDS HSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFG+GPSQVASA
Sbjct: 64  TSLRFHENFDFDHDSVHSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFGVGPSQVASA 123

Query: 171 CPEVPLSPRFQDATLTSPNSDSPSPGSPIPSVGRTGIIKISPSVILLPDTPSGSQQKTNQ 230
           CPEVP+SPRFQDATLTSPNSDSPSPGSP+PSVG+TGI KISPSVILLPDTPSGSQQKTNQ
Sbjct: 124 CPEVPMSPRFQDATLTSPNSDSPSPGSPMPSVGQTGINKISPSVILLPDTPSGSQQKTNQ 183

Query: 231 SEGLSFPDSIKSKWSATSAKYKESILRSTQGIKEKLLARNSSVKELSKGVKREVSAGIAG 290
           SEGLSF DSIKSKWSATSAKYKESILRSTQGIKEKLLARNSSVKELSKGVKREVSAGIAG
Sbjct: 184 SEGLSFQDSIKSKWSATSAKYKESILRSTQGIKEKLLARNSSVKELSKGVKREVSAGIAG 243

Query: 291 VARMIDRLDLTSKRNTGSVSFFSCGGGTSNSLKGKNVVQESAVTDGSVKIDRICTGSPSQ 350
           VARMIDRLDLTSKRNTGSVSFFSC GGTSNSLKGKNVVQESA TDGSVKI+RICTGSPSQ
Sbjct: 244 VARMIDRLDLTSKRNTGSVSFFSC-GGTSNSLKGKNVVQESAGTDGSVKINRICTGSPSQ 303

Query: 351 VSPTVPGSVEVSLAQRGD 369
           VSPTVPGSVEVSLAQRGD
Sbjct: 304 VSPTVPGSVEVSLAQRGD 320

[Close]

BLAST of PI0000770 vs. ExPASy TrEMBL
Match: A0A6J1H5S5 (E3 ubiquitin-protein ligase RHF1A isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111460372 PE=4 SV=1)

HSP 1 Score: 563.9 bits (1452), Expect = 4.9e-157
Identity = 313/376 (83.24%), Postives = 330/376 (87.77%), Query Frame = 0


Query: 1   MANFTSSSSSSSFSLNPNAVSASAAAASDDDF-DDACCICLDPFTSDDPATITSCKHEYH 60
           MA+FTSSS         NA+S      SDD F DDACCICLDPFTS DP +ITSCKHEYH
Sbjct: 1   MADFTSSSL--------NALS----PPSDDAFEDDACCICLDPFTSHDPPSITSCKHEYH 60

Query: 61  LQCILDWSQRSDECPICCQLLVLKDPVGQELLAAMANERLLKSRSLSSAASTSLRFHENF 120
           LQCILDWSQRS+ECPICCQLLVL+DPVGQELLAA+ANE+LLKSRSLSS ASTSLRFHENF
Sbjct: 61  LQCILDWSQRSNECPICCQLLVLRDPVGQELLAAIANEKLLKSRSLSSVASTSLRFHENF 120

Query: 121 DIDHDSAHSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFGLGPSQVASACPE------ 180
           D+DHDS+ SDDSDFDDLIMQHLAAAASRARYVHRRERQR FGLGPSQ ASACPE      
Sbjct: 121 DVDHDSSRSDDSDFDDLIMQHLAAAASRARYVHRRERQRQFGLGPSQGASACPEEVPVLI 180

Query: 181 VPLSPRFQDATLTSPNSDSPSPGSPIPSVGRTGIIKISPSVILLPDTPSGSQQKTNQSEG 240
            PLSPRFQDA+L+SPNSDSPSPGSP+ S GRTGI KISP V LL +T SGSQQKTNQSEG
Sbjct: 181 CPLSPRFQDASLSSPNSDSPSPGSPLSSGGRTGISKISPRV-LLTETSSGSQQKTNQSEG 240

Query: 241 LSFPDSIKSKWSATSAKYKESILRSTQGIKEKLLARNSSVKELSKGVKREVSAGIAGVAR 300
           LSFPDSIKSKWSATSAKYKESI RSTQGIKEKLLARNSSVKELS+GVKREVSAGIAGVAR
Sbjct: 241 LSFPDSIKSKWSATSAKYKESIWRSTQGIKEKLLARNSSVKELSRGVKREVSAGIAGVAR 300

Query: 301 MIDRLDLTSKRNTGSVSFFSCGGGTSNSLKGKNVV-QESAVTDGSVKIDRICTGSPSQVS 360
           MIDRLDL+SKRN  SVSFFSC GGTSNS+KGKNVV QES VTDGSVK DRICTGSPS VS
Sbjct: 301 MIDRLDLSSKRNAASVSFFSCSGGTSNSMKGKNVVMQESGVTDGSVKNDRICTGSPSHVS 360

Query: 361 PTVPGSVEVSLAQRGD 369
           PTVPGSV+ SLAQRGD
Sbjct: 361 PTVPGSVDDSLAQRGD 363

[Close]

BLAST of PI0000770 vs. ExPASy TrEMBL
Match: A0A6J1L048 (E3 ubiquitin-protein ligase RHF1A isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111499166 PE=4 SV=1)

HSP 1 Score: 562.8 bits (1449), Expect = 1.1e-156
Identity = 312/376 (82.98%), Postives = 329/376 (87.50%), Query Frame = 0


Query: 1   MANFTSSSSSSSFSLNPNAVSASAAAASDDDF-DDACCICLDPFTSDDPATITSCKHEYH 60
           MA+FTSSS         NA+S      SDD F DDACCICLDPFTS DP +ITSCKHEYH
Sbjct: 1   MADFTSSSL--------NALS----PPSDDAFEDDACCICLDPFTSHDPPSITSCKHEYH 60

Query: 61  LQCILDWSQRSDECPICCQLLVLKDPVGQELLAAMANERLLKSRSLSSAASTSLRFHENF 120
           LQCILDWSQRS+ECPICCQLLVL+DPVGQELLAA+A+E+LLKSRSLSS ASTSLRFHENF
Sbjct: 61  LQCILDWSQRSNECPICCQLLVLRDPVGQELLAAIASEKLLKSRSLSSVASTSLRFHENF 120

Query: 121 DIDHDSAHSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFGLGPSQVASACPE------ 180
           D+DHDS+HSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFGLGPSQ  SACPE      
Sbjct: 121 DVDHDSSHSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFGLGPSQGVSACPEEVPVLI 180

Query: 181 VPLSPRFQDATLTSPNSDSPSPGSPIPSVGRTGIIKISPSVILLPDTPSGSQQKTNQSEG 240
            PLSPRFQDATL+SPNSDSPSPGSP+ S GRTGI KISP V LL +T SGSQQKTNQ EG
Sbjct: 181 CPLSPRFQDATLSSPNSDSPSPGSPLSSGGRTGISKISPRV-LLTETLSGSQQKTNQPEG 240

Query: 241 LSFPDSIKSKWSATSAKYKESILRSTQGIKEKLLARNSSVKELSKGVKREVSAGIAGVAR 300
           LSFPDSIKSKWSATSAKYKESI RSTQGIKEKLLARNSSVKELS+GVKREVSAGIAGVAR
Sbjct: 241 LSFPDSIKSKWSATSAKYKESIWRSTQGIKEKLLARNSSVKELSRGVKREVSAGIAGVAR 300

Query: 301 MIDRLDLTSKRNTGSVSFFSCGGGTSNSLKGKNVV-QESAVTDGSVKIDRICTGSPSQVS 360
           MIDRLDL+SKRN  SVSFFSC GGTSNS+KGKNVV QES VTDGSVK DRICTGSPS VS
Sbjct: 301 MIDRLDLSSKRNAASVSFFSCSGGTSNSMKGKNVVMQESGVTDGSVKNDRICTGSPSHVS 360

Query: 361 PTVPGSVEVSLAQRGD 369
           PTVPGSV  SLAQRG+
Sbjct: 361 PTVPGSVNDSLAQRGN 363

[Close]

BLAST of PI0000770 vs. NCBI nr
Match: XP_008458566.1 (PREDICTED: E3 ubiquitin-protein ligase RHF1A [Cucumis melo])

HSP 1 Score: 656.4 bits (1692), Expect = 1.5e-184
Identity = 353/371 (95.15%), Postives = 359/371 (96.77%), Query Frame = 0


Query: 1   MANFTSSS--SSSSFSLNPNAVSAS-AAAASDDDFDDACCICLDPFTSDDPATITSCKHE 60
           MANFTSSS  S SS SLNPNAVSAS AAAASDDDFDDACCICLDPFTSDDPATITSCKHE
Sbjct: 1   MANFTSSSSPSPSSVSLNPNAVSASAAAAASDDDFDDACCICLDPFTSDDPATITSCKHE 60

Query: 61  YHLQCILDWSQRSDECPICCQLLVLKDPVGQELLAAMANERLLKSRSLSSAASTSLRFHE 120
           YHLQCILDWSQRSDECPICCQLL LKDPVGQELLAAMA+ERLLKSRSLSSAASTSLRFHE
Sbjct: 61  YHLQCILDWSQRSDECPICCQLLALKDPVGQELLAAMASERLLKSRSLSSAASTSLRFHE 120

Query: 121 NFDIDHDSAHSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFGLGPSQVASACPEVPLS 180
           NFD DHDS HSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFG+GPSQVASACPEVP+S
Sbjct: 121 NFDFDHDSVHSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFGVGPSQVASACPEVPMS 180

Query: 181 PRFQDATLTSPNSDSPSPGSPIPSVGRTGIIKISPSVILLPDTPSGSQQKTNQSEGLSFP 240
           PRFQDATLTSPNSDSPSPGSP+PSVG+TGI KISPSVILLPDTPSGSQQKTNQSEGLSF 
Sbjct: 181 PRFQDATLTSPNSDSPSPGSPMPSVGQTGINKISPSVILLPDTPSGSQQKTNQSEGLSFQ 240

Query: 241 DSIKSKWSATSAKYKESILRSTQGIKEKLLARNSSVKELSKGVKREVSAGIAGVARMIDR 300
           DSIKSKWSATSAKYKESILRSTQGIKEKLLARNSSVKELSKGVKREVSAGIAGVARMIDR
Sbjct: 241 DSIKSKWSATSAKYKESILRSTQGIKEKLLARNSSVKELSKGVKREVSAGIAGVARMIDR 300

Query: 301 LDLTSKRNTGSVSFFSCGGGTSNSLKGKNVVQESAVTDGSVKIDRICTGSPSQVSPTVPG 360
           LDLTSKRNTGSVSFFSC GGTSNSLKGKNVVQESA TDGSVKI+RICTGSPSQVSPTVPG
Sbjct: 301 LDLTSKRNTGSVSFFSC-GGTSNSLKGKNVVQESAGTDGSVKINRICTGSPSQVSPTVPG 360

Query: 361 SVEVSLAQRGD 369
           SVEVSLAQRGD
Sbjct: 361 SVEVSLAQRGD 370

[Close]

BLAST of PI0000770 vs. NCBI nr
Match: XP_004149264.1 (E3 ubiquitin-protein ligase RHF1A [Cucumis sativus] >KGN46964.1 hypothetical protein Csa_020594 [Cucumis sativus])

HSP 1 Score: 647.9 bits (1670), Expect = 5.3e-182
Identity = 345/368 (93.75%), Postives = 352/368 (95.65%), Query Frame = 0


Query: 1   MANFTSSSSSSSFSLNPNAVSASAAAASDDDFDDACCICLDPFTSDDPATITSCKHEYHL 60
           MANFT  SSSS  SLNPNAVSAS AAASDDDFDD CCICLDPFTSDDPATITSCKHEYHL
Sbjct: 1   MANFT--SSSSFVSLNPNAVSAS-AAASDDDFDDVCCICLDPFTSDDPATITSCKHEYHL 60

Query: 61  QCILDWSQRSDECPICCQLLVLKDPVGQELLAAMANERLLKSRSLSSAASTSLRFHENFD 120
           QCILDWSQRSDECPICCQLLVLKDPVGQELLAAMA+ERLLKSR LSSAASTSL FHENFD
Sbjct: 61  QCILDWSQRSDECPICCQLLVLKDPVGQELLAAMASERLLKSRGLSSAASTSLHFHENFD 120

Query: 121 IDHDSAHSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFGLGPSQVASACPEVPLSPRF 180
            DHDS HSDDSDFDDLIMQHLAAAASRARYV RRERQRHFG+GPSQV+SACPEVP+SPRF
Sbjct: 121 FDHDSVHSDDSDFDDLIMQHLAAAASRARYVQRRERQRHFGVGPSQVSSACPEVPMSPRF 180

Query: 181 QDATLTSPNSDSPSPGSPIPSVGRTGIIKISPSVILLPDTPSGSQQKTNQSEGLSFPDSI 240
           QDATLTSPNSDSPSP SP+PSVGRTGI KISPSVILLPDTPSGSQQKTNQSEGLSF DSI
Sbjct: 181 QDATLTSPNSDSPSPSSPMPSVGRTGINKISPSVILLPDTPSGSQQKTNQSEGLSFQDSI 240

Query: 241 KSKWSATSAKYKESILRSTQGIKEKLLARNSSVKELSKGVKREVSAGIAGVARMIDRLDL 300
           KSKWSATSAKYKESILRSTQGIKEKLLARNSSVKELSKGVKREVSAGIAGVARMIDRLDL
Sbjct: 241 KSKWSATSAKYKESILRSTQGIKEKLLARNSSVKELSKGVKREVSAGIAGVARMIDRLDL 300

Query: 301 TSKRNTGSVSFFSCGGGTSNSLKGKNVVQESAVTDGSVKIDRICTGSPSQVSPTVPGSVE 360
           TSKRNTGSVSFFSC GGTSNSLKGKNVVQESAVTDGSVKI+RICTGSPSQVSPTVP SV+
Sbjct: 301 TSKRNTGSVSFFSCSGGTSNSLKGKNVVQESAVTDGSVKINRICTGSPSQVSPTVPSSVK 360

Query: 361 VSLAQRGD 369
           VSLAQRGD
Sbjct: 361 VSLAQRGD 365

[Close]

BLAST of PI0000770 vs. NCBI nr
Match: XP_038906575.1 (E3 ubiquitin-protein ligase RHF1A [Benincasa hispida])

HSP 1 Score: 624.0 bits (1608), Expect = 8.2e-175
Identity = 340/369 (92.14%), Postives = 348/369 (94.31%), Query Frame = 0


Query: 1   MANFTSSSSSSSFSLNPNAVSASAAAASDDDFDDACCICLDPFTSDDPATITSCKHEYHL 60
           MANFTSSSSS     NPNAVSAS  AASDD FDDACCICLDPFTSDDPATITSCKHEYHL
Sbjct: 1   MANFTSSSSSH----NPNAVSAS--AASDDAFDDACCICLDPFTSDDPATITSCKHEYHL 60

Query: 61  QCILDWSQRSDECPICCQLLVLKDPVGQELLAAMANERLLKSRSLSSAASTSLRFHENFD 120
           QCILDWSQRS+ECPICCQLLVLKDPVGQELLAA+A+ERLLK RSLSSAASTSLRFHENFD
Sbjct: 61  QCILDWSQRSNECPICCQLLVLKDPVGQELLAAIASERLLKLRSLSSAASTSLRFHENFD 120

Query: 121 IDHDSAHSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFGLGPSQVASACP-EVPLSPR 180
           IDHDSAHSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFGLGPSQV SACP EVP+SPR
Sbjct: 121 IDHDSAHSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFGLGPSQVISACPEEVPMSPR 180

Query: 181 FQDATLTSPNSDSPSPGSPIPSVGRTGIIKISPSVILLPDTPSGSQQKTNQSEGLSFPDS 240
           FQDATL SPNSDSPSPGSP+ SVG+TG  KIS SVILLP  PSGS QKTNQSEGLSFPDS
Sbjct: 181 FQDATLISPNSDSPSPGSPLSSVGQTGTSKISHSVILLP--PSGSHQKTNQSEGLSFPDS 240

Query: 241 IKSKWSATSAKYKESILRSTQGIKEKLLARNSSVKELSKGVKREVSAGIAGVARMIDRLD 300
           IKSKWSATSAKYKESILRSTQGIKEKLLARNSS+KELS+GVKREVSAGIAGVARMIDRLD
Sbjct: 241 IKSKWSATSAKYKESILRSTQGIKEKLLARNSSMKELSRGVKREVSAGIAGVARMIDRLD 300

Query: 301 LTSKRNTGSVSFFSCGGGTSNSLKGKNVVQESAVTDGSVKIDRICTGSPSQVSPTVPGSV 360
           LTSKRNTGSVSFFS  GGTSNSLKGKNVVQESAVTDGSVKIDRICTGSPSQVSPTVPGSV
Sbjct: 301 LTSKRNTGSVSFFSFSGGTSNSLKGKNVVQESAVTDGSVKIDRICTGSPSQVSPTVPGSV 360

Query: 361 EVSLAQRGD 369
           EVSLAQRGD
Sbjct: 361 EVSLAQRGD 361

[Close]

BLAST of PI0000770 vs. NCBI nr
Match: KAA0033417.1 (E3 ubiquitin-protein ligase RHF1A [Cucumis melo var. makuwa])

HSP 1 Score: 582.8 bits (1501), Expect = 2.1e-162
Identity = 305/318 (95.91%), Postives = 311/318 (97.80%), Query Frame = 0


Query: 51  ITSCKHEYHLQCILDWSQRSDECPICCQLLVLKDPVGQELLAAMANERLLKSRSLSSAAS 110
           ITSCKHEYHLQCILDWSQRSDECPICCQLL LKDPVGQELLAAMA+ERLLKSRSLSSAAS
Sbjct: 4   ITSCKHEYHLQCILDWSQRSDECPICCQLLALKDPVGQELLAAMASERLLKSRSLSSAAS 63

Query: 111 TSLRFHENFDIDHDSAHSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFGLGPSQVASA 170
           TSLRFHENFD DHDS HSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFG+GPSQVASA
Sbjct: 64  TSLRFHENFDFDHDSVHSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFGVGPSQVASA 123

Query: 171 CPEVPLSPRFQDATLTSPNSDSPSPGSPIPSVGRTGIIKISPSVILLPDTPSGSQQKTNQ 230
           CPEVP+SPRFQDATLTSPNSDSPSPGSP+PSVG+TGI KISPSVILLPDTPSGSQQKTNQ
Sbjct: 124 CPEVPMSPRFQDATLTSPNSDSPSPGSPMPSVGQTGINKISPSVILLPDTPSGSQQKTNQ 183

Query: 231 SEGLSFPDSIKSKWSATSAKYKESILRSTQGIKEKLLARNSSVKELSKGVKREVSAGIAG 290
           SEGLSF DSIKSKWSATSAKYKESILRSTQGIKEKLLARNSSVKELSKGVKREVSAGIAG
Sbjct: 184 SEGLSFQDSIKSKWSATSAKYKESILRSTQGIKEKLLARNSSVKELSKGVKREVSAGIAG 243

Query: 291 VARMIDRLDLTSKRNTGSVSFFSCGGGTSNSLKGKNVVQESAVTDGSVKIDRICTGSPSQ 350
           VARMIDRLDLTSKRNTGSVSFFSC GGTSNSLKGKNVVQESA TDGSVKI+RICTGSPSQ
Sbjct: 244 VARMIDRLDLTSKRNTGSVSFFSC-GGTSNSLKGKNVVQESAGTDGSVKINRICTGSPSQ 303

Query: 351 VSPTVPGSVEVSLAQRGD 369
           VSPTVPGSVEVSLAQRGD
Sbjct: 304 VSPTVPGSVEVSLAQRGD 320

[Close]

BLAST of PI0000770 vs. NCBI nr
Match: XP_022959383.1 (E3 ubiquitin-protein ligase RHF1A isoform X1 [Cucurbita moschata])

HSP 1 Score: 563.9 bits (1452), Expect = 1.0e-156
Identity = 313/376 (83.24%), Postives = 330/376 (87.77%), Query Frame = 0


Query: 1   MANFTSSSSSSSFSLNPNAVSASAAAASDDDF-DDACCICLDPFTSDDPATITSCKHEYH 60
           MA+FTSSS         NA+S      SDD F DDACCICLDPFTS DP +ITSCKHEYH
Sbjct: 1   MADFTSSSL--------NALS----PPSDDAFEDDACCICLDPFTSHDPPSITSCKHEYH 60

Query: 61  LQCILDWSQRSDECPICCQLLVLKDPVGQELLAAMANERLLKSRSLSSAASTSLRFHENF 120
           LQCILDWSQRS+ECPICCQLLVL+DPVGQELLAA+ANE+LLKSRSLSS ASTSLRFHENF
Sbjct: 61  LQCILDWSQRSNECPICCQLLVLRDPVGQELLAAIANEKLLKSRSLSSVASTSLRFHENF 120

Query: 121 DIDHDSAHSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFGLGPSQVASACPE------ 180
           D+DHDS+ SDDSDFDDLIMQHLAAAASRARYVHRRERQR FGLGPSQ ASACPE      
Sbjct: 121 DVDHDSSRSDDSDFDDLIMQHLAAAASRARYVHRRERQRQFGLGPSQGASACPEEVPVLI 180

Query: 181 VPLSPRFQDATLTSPNSDSPSPGSPIPSVGRTGIIKISPSVILLPDTPSGSQQKTNQSEG 240
            PLSPRFQDA+L+SPNSDSPSPGSP+ S GRTGI KISP V LL +T SGSQQKTNQSEG
Sbjct: 181 CPLSPRFQDASLSSPNSDSPSPGSPLSSGGRTGISKISPRV-LLTETSSGSQQKTNQSEG 240

Query: 241 LSFPDSIKSKWSATSAKYKESILRSTQGIKEKLLARNSSVKELSKGVKREVSAGIAGVAR 300
           LSFPDSIKSKWSATSAKYKESI RSTQGIKEKLLARNSSVKELS+GVKREVSAGIAGVAR
Sbjct: 241 LSFPDSIKSKWSATSAKYKESIWRSTQGIKEKLLARNSSVKELSRGVKREVSAGIAGVAR 300

Query: 301 MIDRLDLTSKRNTGSVSFFSCGGGTSNSLKGKNVV-QESAVTDGSVKIDRICTGSPSQVS 360
           MIDRLDL+SKRN  SVSFFSC GGTSNS+KGKNVV QES VTDGSVK DRICTGSPS VS
Sbjct: 301 MIDRLDLSSKRNAASVSFFSCSGGTSNSMKGKNVVMQESGVTDGSVKNDRICTGSPSHVS 360

Query: 361 PTVPGSVEVSLAQRGD 369
           PTVPGSV+ SLAQRGD
Sbjct: 361 PTVPGSVDDSLAQRGD 363

[Close]

BLAST of PI0000770 vs. TAIR 10
Match: AT4G14220.1 (RING-H2 group F1A )

HSP 1 Score: 240.4 bits (612), Expect = 2.4e-63
Identity = 168/368 (45.65%), Postives = 223/368 (60.60%), Query Frame = 0


Query: 1   MANFTSSSS---SSSFSLNPNAVSASAAA-----ASDDD--FDDACCICLDPFTSDDPAT 60
           M+NFT +S+   S +   NP   S+S+++     ASDDD   DDAC ICL+PFT  DP+T
Sbjct: 1   MSNFTYTSAFNLSDNSPFNPAIGSSSSSSSALVVASDDDNNTDDACSICLEPFTLQDPST 60

Query: 61  ITSCKHEYHLQCILDWSQRSDECPICCQLLVLKDPVGQELLAAMANERLLKSRSLSSAAS 120
           +TSCKHEYHLQCI++WSQRS ECPIC QL VL+DP  QELLAA+  ERLLK+R++SS++ 
Sbjct: 61  VTSCKHEYHLQCIIEWSQRSKECPICWQLFVLRDPASQELLAAVEKERLLKTRNISSSSP 120

Query: 121 TSLRFHENFDIDHDSAHSDDSDFDDLIMQHLAAAASRARYVHRRERQRHFGLGPSQVASA 180
            S+  H + D   +   S  S FD+  ++HL  AA R   + RR+ Q    L  S   + 
Sbjct: 121 ISIH-HSHDDFHSEEEESQFSSFDEQFLRHLTEAAHRRCLLRRRDGQISSSLVSSSDPTT 180

Query: 181 CPEVPLSPRFQDATLT-----------SPNSDSPSP---GSPIPSVGRTGIIKISPSVIL 240
                L   ++ + ++           SP S +PSP    S IP+    G  +ISP    
Sbjct: 181 IHPTDLVNLYRLSAISHVEHQNSNPCPSPGSMTPSPVSGHSSIPADSNNG-SRISPG--- 240

Query: 241 LPDTPSGSQQKTNQSEGLSFPDSIKSKWSATSAKYKESILRSTQGIKEKLLARNSSVKEL 300
              +PS S Q     E  S P++IKSK +A SAKYKESI +S QG+KEKLLARN+SVKEL
Sbjct: 241 --PSPSRSSQSPKSPEASSLPEAIKSKLAAASAKYKESISKSKQGLKEKLLARNNSVKEL 300

Query: 301 SKGVKREVSAGIAGVARMIDRLDLTSKRNTGSV---SFFSCGGGTSNSLKGKNVVQESAV 342
           SKGV+RE++AGIAGVARMI+R+D +SKR  GS    +  +   G + S KGK V   S  
Sbjct: 301 SKGVQREMNAGIAGVARMIERMDFSSKRFGGSAHVSTSTATASGFNFSFKGKRVEANSKS 360

[Close]

BLAST of PI0000770 vs. TAIR 10
Match: AT5G22000.2 (RING-H2 group F2A )

HSP 1 Score: 166.4 bits (420), Expect = 4.3e-41
Identity = 137/368 (37.23%), Postives = 196/368 (53.26%), Query Frame = 0


Query: 19  AVSASAAAASDDDFDDACCICLDPFTSDDPATITSCKHEYHLQCILDWSQRSDECPICCQ 78
           + +A       D  DDAC ICL+ F   DP+T+TSCKHEYHLQCIL+W QRS +CP+C Q
Sbjct: 16  SAAAFVEGGIQDACDDACSICLESFCESDPSTLTSCKHEYHLQCILEWCQRSSQCPMCWQ 75

Query: 79  LLVLKDPVGQELLAAMANERLLKSRSLSSAASTSLRFHENFDIDHDSAHSDDSDFDDLIM 138
            + LKDP  QELL A+  ER  +     +A         +F++ H     D+++ ++ I+
Sbjct: 76  SISLKDPTSQELLEAVEQERNFRFNPTRNATIFRHPTLGDFELQHLPVGVDNAEIEERII 135

Query: 139 QHLAAAAS--RARYVHRRERQR---------HFGLGPSQVASAC----PEVPLSPRFQDA 198
           QHLAAAA+  RAR+  RRE  R          F +  SQ  ++     P +P SP  +D 
Sbjct: 136 QHLAAAAAMGRARHGVRREGHRSRSSSQGHQQFMVFSSQPNASSPPPHPPMPSSPSQRDE 195

Query: 199 TLTSPN------------SDSPSPGSPIPSVGRTGIIKISPSVI------LLPDTPSGSQ 258
           + T  N            S++  P S  P        ++SPS        L   +PS  Q
Sbjct: 196 SDTVSNLPHNALGEGSHQSNTQPPTSSHPR-------QVSPSASDSNSRPLNQSSPS-EQ 255

Query: 259 QKTNQSEGLSFPDSIKSKWSATSAKYKESILRSTQGIKEKLLARNSSVKELSKGVKREVS 318
            +   SE  SF +S+KS+ +A S +YKESI ++T+  K++L +RN+S+ +L   VKREVS
Sbjct: 256 DRAGPSELQSFSESLKSRLNAVSTRYKESISKNTRNWKDRLFSRNTSMADLGSEVKREVS 315

Query: 319 AGIAGVARMIDRLDL--TSKRNTGSVSFFSCG--GGTSNSLKGKNVVQESAVTDGSVKID 350
           AGIA V+RM++RL+    S+ +T SVS  S      T+N         E +V +  VK +
Sbjct: 316 AGIATVSRMMERLETRENSRPSTASVSDVSENHTPETNNEHNRAAAGDEHSVNERGVK-E 374

[Close]

BLAST of PI0000770 vs. TAIR 10
Match: AT5G22000.1 (RING-H2 group F2A )

HSP 1 Score: 166.4 bits (420), Expect = 4.3e-41
Identity = 137/368 (37.23%), Postives = 196/368 (53.26%), Query Frame = 0


Query: 19  AVSASAAAASDDDFDDACCICLDPFTSDDPATITSCKHEYHLQCILDWSQRSDECPICCQ 78
           + +A       D  DDAC ICL+ F   DP+T+TSCKHEYHLQCIL+W QRS +CP+C Q
Sbjct: 16  SAAAFVEGGIQDACDDACSICLESFCESDPSTLTSCKHEYHLQCILEWCQRSSQCPMCWQ 75

Query: 79  LLVLKDPVGQELLAAMANERLLKSRSLSSAASTSLRFHENFDIDHDSAHSDDSDFDDLIM 138
            + LKDP  QELL A+  ER  +     +A         +F++ H     D+++ ++ I+
Sbjct: 76  SISLKDPTSQELLEAVEQERNFRFNPTRNATIFRHPTLGDFELQHLPVGVDNAEIEERII 135

Query: 139 QHLAAAAS--RARYVHRRERQR---------HFGLGPSQVASAC----PEVPLSPRFQDA 198
           QHLAAAA+  RAR+  RRE  R          F +  SQ  ++     P +P SP  +D 
Sbjct: 136 QHLAAAAAMGRARHGVRREGHRSRSSSQGHQQFMVFSSQPNASSPPPHPPMPSSPSQRDE 195

Query: 199 TLTSPN------------SDSPSPGSPIPSVGRTGIIKISPSVI------LLPDTPSGSQ 258
           + T  N            S++  P S  P        ++SPS        L   +PS  Q
Sbjct: 196 SDTVSNLPHNALGEGSHQSNTQPPTSSHPR-------QVSPSASDSNSRPLNQSSPS-EQ 255

Query: 259 QKTNQSEGLSFPDSIKSKWSATSAKYKESILRSTQGIKEKLLARNSSVKELSKGVKREVS 318
            +   SE  SF +S+KS+ +A S +YKESI ++T+  K++L +RN+S+ +L   VKREVS
Sbjct: 256 DRAGPSELQSFSESLKSRLNAVSTRYKESISKNTRNWKDRLFSRNTSMADLGSEVKREVS 315

Query: 319 AGIAGVARMIDRLDL--TSKRNTGSVSFFSCG--GGTSNSLKGKNVVQESAVTDGSVKID 350
           AGIA V+RM++RL+    S+ +T SVS  S      T+N         E +V +  VK +
Sbjct: 316 AGIATVSRMMERLETRENSRPSTASVSDVSENHTPETNNEHNRAAAGDEHSVNERGVK-E 374

[Close]

BLAST of PI0000770 vs. TAIR 10
Match: AT5G22000.3 (RING-H2 group F2A )

HSP 1 Score: 166.4 bits (420), Expect = 4.3e-41
Identity = 137/368 (37.23%), Postives = 196/368 (53.26%), Query Frame = 0


Query: 19  AVSASAAAASDDDFDDACCICLDPFTSDDPATITSCKHEYHLQCILDWSQRSDECPICCQ 78
           + +A       D  DDAC ICL+ F   DP+T+TSCKHEYHLQCIL+W QRS +CP+C Q
Sbjct: 16  SAAAFVEGGIQDACDDACSICLESFCESDPSTLTSCKHEYHLQCILEWCQRSSQCPMCWQ 75

Query: 79  LLVLKDPVGQELLAAMANERLLKSRSLSSAASTSLRFHENFDIDHDSAHSDDSDFDDLIM 138
            + LKDP  QELL A+  ER  +     +A         +F++ H     D+++ ++ I+
Sbjct: 76  SISLKDPTSQELLEAVEQERNFRFNPTRNATIFRHPTLGDFELQHLPVGVDNAEIEERII 135

Query: 139 QHLAAAAS--RARYVHRRERQR---------HFGLGPSQVASAC----PEVPLSPRFQDA 198
           QHLAAAA+  RAR+  RRE  R          F +  SQ  ++     P +P SP  +D 
Sbjct: 136 QHLAAAAAMGRARHGVRREGHRSRSSSQGHQQFMVFSSQPNASSPPPHPPMPSSPSQRDE 195

Query: 199 TLTSPN------------SDSPSPGSPIPSVGRTGIIKISPSVI------LLPDTPSGSQ 258
           + T  N            S++  P S  P        ++SPS        L   +PS  Q
Sbjct: 196 SDTVSNLPHNALGEGSHQSNTQPPTSSHPR-------QVSPSASDSNSRPLNQSSPS-EQ 255

Query: 259 QKTNQSEGLSFPDSIKSKWSATSAKYKESILRSTQGIKEKLLARNSSVKELSKGVKREVS 318
            +   SE  SF +S+KS+ +A S +YKESI ++T+  K++L +RN+S+ +L   VKREVS
Sbjct: 256 DRAGPSELQSFSESLKSRLNAVSTRYKESISKNTRNWKDRLFSRNTSMADLGSEVKREVS 315

Query: 319 AGIAGVARMIDRLDL--TSKRNTGSVSFFSCG--GGTSNSLKGKNVVQESAVTDGSVKID 350
           AGIA V+RM++RL+    S+ +T SVS  S      T+N         E +V +  VK +
Sbjct: 316 AGIATVSRMMERLETRENSRPSTASVSDVSENHTPETNNEHNRAAAGDEHSVNERGVK-E 374

[Close]

BLAST of PI0000770 vs. TAIR 10
Match: AT4G00335.1 (RING-H2 finger B1A )

HSP 1 Score: 63.5 bits (153), Expect = 4.0e-10
Identity = 24/55 (43.64%), Postives = 37/55 (67.27%), Query Frame = 0


Query: 30  DDFDDACCICLDPFTSDDPATITSCKHEYHLQCILDWSQRSDECPICCQLLVLKD 85
           D+ +D C IC + +  ++P   T C+HE+HL C+L+W +RSD CPIC + +V  D
Sbjct: 133 DEEEDCCPICFEDYDVENPRLTTKCEHEFHLSCLLEWIERSDRCPICDKEVVFDD 187

The following BLAST results are available for this feature:

BLAST of PI0000770 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q4TU14	3.3e-62	45.65	E3 ubiquitin-protein ligase RHF1A OS=Arabidopsis thaliana OX=3702 GN=RHF1A PE=1 ...	[more]
Q9ZT42	6.1e-40	37.23	E3 ubiquitin-protein ligase RHF2A OS=Arabidopsis thaliana OX=3702 GN=RHF2A PE=2 ...	[more]
Q2HIJ8	5.6e-09	43.64	Probable E3 ubiquitin-protein ligase RHB1A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]
Q93ZF6	2.1e-08	47.06	E3 ubiquitin-protein ligase AIRP1 OS=Arabidopsis thaliana OX=3702 GN=AIRP1 PE=1 ...	[more]
Q8LE94	2.1e-08	41.67	E3 ubiquitin-protein ligase At3g02290 OS=Arabidopsis thaliana OX=3702 GN=At3g022...	[more]

BLAST of PI0000770 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3C9D8	7.2e-185	95.15	E3 ubiquitin-protein ligase RHF1A OS=Cucumis melo OX=3656 GN=LOC103497932 PE=4 S...	[more]
A0A0A0KDQ6	2.6e-182	93.75	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G153470 P...	[more]
A0A5A7SVT0	1.0e-162	95.91	E3 ubiquitin-protein ligase RHF1A OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2...	[more]
A0A6J1H5S5	4.9e-157	83.24	E3 ubiquitin-protein ligase RHF1A isoform X1 OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A6J1L048	1.1e-156	82.98	E3 ubiquitin-protein ligase RHF1A isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]

BLAST of PI0000770 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008458566.1	1.5e-184	95.15	PREDICTED: E3 ubiquitin-protein ligase RHF1A [Cucumis melo]	[more]
XP_004149264.1	5.3e-182	93.75	E3 ubiquitin-protein ligase RHF1A [Cucumis sativus] >KGN46964.1 hypothetical pro...	[more]
XP_038906575.1	8.2e-175	92.14	E3 ubiquitin-protein ligase RHF1A [Benincasa hispida]	[more]
KAA0033417.1	2.1e-162	95.91	E3 ubiquitin-protein ligase RHF1A [Cucumis melo var. makuwa]	[more]
XP_022959383.1	1.0e-156	83.24	E3 ubiquitin-protein ligase RHF1A isoform X1 [Cucurbita moschata]	[more]

BLAST of PI0000770 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G14220.1	2.4e-63	45.65	RING-H2 group F1A	[more]
AT5G22000.2	4.3e-41	37.23	RING-H2 group F2A	[more]
AT5G22000.1	4.3e-41	37.23	RING-H2 group F2A	[more]
AT5G22000.3	4.3e-41	37.23	RING-H2 group F2A	[more]
AT4G00335.1	4.0e-10	43.64	RING-H2 finger B1A	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 36..76 e-value: 5.4E-7 score: 39.2
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 35..76 e-value: 6.9E-9 score: 35.9
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 36..76 score: 11.840146
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 3..81 e-value: 5.7E-14 score: 53.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 344..359
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 344..368
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 218..239
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 178..203
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 180..199
None	No IPR available	PANTHER	PTHR46463	ZINC FINGER, RING/FYVE/PHD-TYPE	coord: 1..340
None	No IPR available	PANTHER	PTHR46463:SF16	E3 UBIQUITIN-PROTEIN LIGASE RHF1A	coord: 1..340
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 33..83

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0000770.1	PI0000770.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009561	megagametogenesis
biological_process	GO:0055046	microgametogenesis
biological_process	GO:0010498	proteasomal protein catabolic process
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0051726	regulation of cell cycle
molecular_function	GO:0004842	ubiquitin-protein transferase activity