PI0000760 (gene) Melon (PI 482460) v1

Overview
NamePI0000760
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionSphingolipid delta(4)-desaturase DES1-like
Locationchr12: 5031298 .. 5034146 (+)
RNA-Seq ExpressionPI0000760
SyntenyPI0000760
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCACCAGAGGCAAGTTGGCAATGGCAACGATACGGAGACGGCGACGTATAAAACAGCGTCCACTCGAACGCAACGTCCTTTCTCTAAGTTTAGGAGTCACCTTCGTTTCCATTTTTCTTTCCATATTTGGTCTTCTTTAATCCTCCTCCCCTTCCTAAATCTTCAACAACCTTCCTCTATAACTATCCCTTCCTTTCTCTTCCTCAATCCACATCCAATTTCATCTTCAGCAATCAGCACCAGCCCCCAGGAGACGCAAACTCTGCCGGAGCATCTTCTGGGAGCAAACGCCCACCGGGAATCACTCACTAACACAATGCAAGGTGGTGAAGAGAGAGATGATGGTGTTATGGCCACTGACTTCTTCTGGTCTTACACCGATGAACCTCACGCTTCCAGGAGACGACAAATTCTCTCTCAGTACCCTCAGATCCGGGAGCTCTTCGGTCCTGACCCCTGGGCTTTCTTCAAGGTTTTTTATTTCTTTTCTCTCTTGATTCTGAGAATATGGAGAGTTGTGGTTATTTTCTTGTCAGGATGTTTTGATTTCGTGTTTAGATCTGTATTGCTGGTCTCCACACATGTCAATGCCTGAATTTGATCTATGTATATTTGTTTTTTTGTTTGATTTTGAACCTCATCTCTGCTACATTGCTTCGTTTTACTTCGTTTTTGTTTTTCTTTTATCATTCTGATTACTCGTGTTTGTTGCCTCCATCGAAAATCTGCATTTGAGATTCTGAACGCTTGTTATTGATGGATCTGTCCACTTCGCTGTTCTTTTTGCTCTGGTTCAGTTATTTTTTTGCTACATTCCTGTATCGTTTATTTATTTGTTTGTTTGTTTCTTCCTTTCTTCCTTCCTTGTATTACGAACACTTGTCTAAACTTTCCTGCCTTTTCAACTATTAGGCAAGCTTTGGTTCAGGATTATCTTGGTTTAAACTTTATTTAAAGTTACCCTTCTTTTCTTATTTGGAATTTAAAAGAATGCCCTGGAGGAAATTGACCCTCAACCTTTTTCACTACGAAATGGTAAAGTTTAATAGTACTCGATGAATTTTGTTGTTTGCTCTCTAGAGTTTTGGTAAATGTTTAAAGAGGAAATTAGCCCTCGACCATTATTTTCTTGGTTGCCTGGCTCTAATATCTGATTTTAGCATATGTTTAATCGTTTGATTAGGGTAAGTCCATTGCAGCAGTTTAGGGCTTGTATAGTTTCAAATGAGACCATTTATGAAATCAGAAGAATAATTGTTGAAGTTAAATATTTAGACTAATGGTTCCTGCTGTTGTAAAGGGAAGACTGATTTTTTTATTGTTTGCTGTACTTGGTAAGAAGAGCAATTGATTGTAATTATTTAACCAACTGCATTGGCATTTTTGATTCATATGAAAAAAGTAAAGTCATGATCACGAAGAATGAGAACTTGGTGTGAACAGCCATTTATACATTCACGAGCATGACCTTGTAGTCATAAAGTTTAAATTAGCTATTTTAGTTTGGAGAACCAATCATTAATTTATTCTTACAGTATAAAAAGTTGGTCATTTTTTTCTTCTCGTTCTCGTTCTCGCATCTTGCAGATTTCTGTGGTTGTTCTGCTTCAGTTATGGACAGCTACGACCCTTCACAGGGCAGACTGGTTAAAGATATTGGCAATAGCCTACTTCTTTGGCTCTTTTCTCAACCACAACCTATTTTTAGCCATTCACGAGCTCAGTCACAACCTTGCCTTCTCAACACCTGACCACAACAGGTGGCTCGGGATTTTTGCTAATCTCCCCATTGGAGTGCCCATGTCCATCACATTTCAAAAGTATCACCTCGAGCACCATCGCTTCCAAGGAGTGGATGGCATGGATATGGACGTTCCAAGCCAGACCGAAGCTCATCTGGTGACTAATGTTGTCTCAAAAGCTATATGGGTTATCTTCCAACTCTTTTTCTATGCACTCCGCCCATTGTTTCTCAAACCAAAGCCCCCAGGTCACTGGGAGTTCATCAACTTCATTATTCAGATAGCTCTTGATGTTGCCATGGTTTACTTCTGGAGTTGGAAGTCTTTTGCTTACCTGATCCTCTCCACATTCGTCGGTGGCGGGATGCATCCAATGGCTGGTCACTTCATTTCCGAACATTATGTCTTCAATCCCAAACAGGAGACGTACTCTTACTACGGTCCACTTAATCTTCTTACATGGAGTGTAGGTTACCATAATGAACACCATGACTTCCCCAGAATTCCAGGAAGTAAGCTTTACAAAGTGAAGGAGATTGCACCAGAATACTACAATGAACTAGATTCATACAAATCATGGAGCCAAGTGATATACATGTACATTATGGACAAGACAGTGGGGCCATTCAGTAGGATGAAGAGAATGCCAAGGAAATCTGAGTAGTTGATCCGTTCTTCTCTCCCCTTGAGATATTCTTTTTGTTCGATTCTTTCTCCATCTTTTCTGATGGATCATTCTCTTTGAGCTGGTTGTGCCCTTGTTTTGTTTACTTACATTCATGGAGAGTTTTCTTTATTCTATTTAGATTGTATAGTAGCCAGGCATGTTCACTGTACTGTATTCGATTTTCATGGCTTTTTGAGGGATTGGTTTGTAAAAGGAGCCAAGAATTAGCGAACCTAGATTCTCATAGGTTTGATATTTTGGTTAATTTATAATTAGTGCCGACTGTGCCTACGTATTTTTATCTACATGTGTCAGAAATCCTAGATGAAACGCCGAGATGCTCAATGCGTAACGTTTGTGATAAAGGTTGAAATTTTGAATGAAGACCAATTAGAGCATATATCTATGCTCATGTATGAAATTGTATGTATGTTAATAAA

mRNA sequence

CCACCAGAGGCAAGTTGGCAATGGCAACGATACGGAGACGGCGACGTATAAAACAGCGTCCACTCGAACGCAACGTCCTTTCTCTAAGTTTAGGAGTCACCTTCGTTTCCATTTTTCTTTCCATATTTGGTCTTCTTTAATCCTCCTCCCCTTCCTAAATCTTCAACAACCTTCCTCTATAACTATCCCTTCCTTTCTCTTCCTCAATCCACATCCAATTTCATCTTCAGCAATCAGCACCAGCCCCCAGGAGACGCAAACTCTGCCGGAGCATCTTCTGGGAGCAAACGCCCACCGGGAATCACTCACTAACACAATGCAAGGTGGTGAAGAGAGAGATGATGGTGTTATGGCCACTGACTTCTTCTGGTCTTACACCGATGAACCTCACGCTTCCAGGAGACGACAAATTCTCTCTCAGTACCCTCAGATCCGGGAGCTCTTCGGTCCTGACCCCTGGGCTTTCTTCAAGATTTCTGTGGTTGTTCTGCTTCAGTTATGGACAGCTACGACCCTTCACAGGGCAGACTGGTTAAAGATATTGGCAATAGCCTACTTCTTTGGCTCTTTTCTCAACCACAACCTATTTTTAGCCATTCACGAGCTCAGTCACAACCTTGCCTTCTCAACACCTGACCACAACAGGTGGCTCGGGATTTTTGCTAATCTCCCCATTGGAGTGCCCATGTCCATCACATTTCAAAAGTATCACCTCGAGCACCATCGCTTCCAAGGAGTGGATGGCATGGATATGGACGTTCCAAGCCAGACCGAAGCTCATCTGGTGACTAATGTTGTCTCAAAAGCTATATGGGTTATCTTCCAACTCTTTTTCTATGCACTCCGCCCATTGTTTCTCAAACCAAAGCCCCCAGGTCACTGGGAGTTCATCAACTTCATTATTCAGATAGCTCTTGATGTTGCCATGGTTTACTTCTGGAGTTGGAAGTCTTTTGCTTACCTGATCCTCTCCACATTCGTCGGTGGCGGGATGCATCCAATGGCTGGTCACTTCATTTCCGAACATTATGTCTTCAATCCCAAACAGGAGACGTACTCTTACTACGGTCCACTTAATCTTCTTACATGGAGTGTAGGTTACCATAATGAACACCATGACTTCCCCAGAATTCCAGGAAGTAAGCTTTACAAAGTGAAGGAGATTGCACCAGAATACTACAATGAACTAGATTCATACAAATCATGGAGCCAAGTGATATACATGTACATTATGGACAAGACAGTGGGGCCATTCAGTAGGATGAAGAGAATGCCAAGGAAATCTGAGTAGTTGATCCGTTCTTCTCTCCCCTTGAGATATTCTTTTTGTTCGATTCTTTCTCCATCTTTTCTGATGGATCATTCTCTTTGAGCTGGTTGTGCCCTTGTTTTGTTTACTTACATTCATGGAGAGTTTTCTTTATTCTATTTAGATTGTATAGTAGCCAGGCATGTTCACTGTACTGTATTCGATTTTCATGGCTTTTTGAGGGATTGGTTTGTAAAAGGAGCCAAGAATTAGCGAACCTAGATTCTCATAGGTTTGATATTTTGGTTAATTTATAATTAGTGCCGACTGTGCCTACGTATTTTTATCTACATGTGTCAGAAATCCTAGATGAAACGCCGAGATGCTCAATGCGTAACGTTTGTGATAAAGGTTGAAATTTTGAATGAAGACCAATTAGAGCATATATCTATGCTCATGTATGAAATTGTATGTATGTTAATAAA

Coding sequence (CDS)

ATGCAAGGTGGTGAAGAGAGAGATGATGGTGTTATGGCCACTGACTTCTTCTGGTCTTACACCGATGAACCTCACGCTTCCAGGAGACGACAAATTCTCTCTCAGTACCCTCAGATCCGGGAGCTCTTCGGTCCTGACCCCTGGGCTTTCTTCAAGATTTCTGTGGTTGTTCTGCTTCAGTTATGGACAGCTACGACCCTTCACAGGGCAGACTGGTTAAAGATATTGGCAATAGCCTACTTCTTTGGCTCTTTTCTCAACCACAACCTATTTTTAGCCATTCACGAGCTCAGTCACAACCTTGCCTTCTCAACACCTGACCACAACAGGTGGCTCGGGATTTTTGCTAATCTCCCCATTGGAGTGCCCATGTCCATCACATTTCAAAAGTATCACCTCGAGCACCATCGCTTCCAAGGAGTGGATGGCATGGATATGGACGTTCCAAGCCAGACCGAAGCTCATCTGGTGACTAATGTTGTCTCAAAAGCTATATGGGTTATCTTCCAACTCTTTTTCTATGCACTCCGCCCATTGTTTCTCAAACCAAAGCCCCCAGGTCACTGGGAGTTCATCAACTTCATTATTCAGATAGCTCTTGATGTTGCCATGGTTTACTTCTGGAGTTGGAAGTCTTTTGCTTACCTGATCCTCTCCACATTCGTCGGTGGCGGGATGCATCCAATGGCTGGTCACTTCATTTCCGAACATTATGTCTTCAATCCCAAACAGGAGACGTACTCTTACTACGGTCCACTTAATCTTCTTACATGGAGTGTAGGTTACCATAATGAACACCATGACTTCCCCAGAATTCCAGGAAGTAAGCTTTACAAAGTGAAGGAGATTGCACCAGAATACTACAATGAACTAGATTCATACAAATCATGGAGCCAAGTGATATACATGTACATTATGGACAAGACAGTGGGGCCATTCAGTAGGATGAAGAGAATGCCAAGGAAATCTGAGTAG

Protein sequence

MQGGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQLWTATTLHRADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPIGVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLFLKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQVIYMYIMDKTVGPFSRMKRMPRKSE
Homology
BLAST of PI0000760 vs. ExPASy Swiss-Prot
Match: Q6H5U3 (Sphingolipid delta(4)-desaturase DES1-like OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0639600 PE=2 SV=1)

HSP 1 Score: 574.3 bits (1479), Expect = 8.6e-163
Identity = 263/322 (81.68%), Postives = 290/322 (90.06%), Query Frame = 0

Query: 3   GGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQLW 62
           G    ++GVMATDFFWSYTDEPHA+RRR+IL+++PQI+ELFGPDP AF KI+ VV LQLW
Sbjct: 6   GDGREEEGVMATDFFWSYTDEPHATRRREILAKHPQIKELFGPDPLAFLKIAAVVSLQLW 65

Query: 63  TATTLHRADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPIGV 122
           TAT L  A W+KIL +AYFFGSFLNHNLFLAIHELSHNLAF+TP +NRWLGIFANLPIGV
Sbjct: 66  TATLLRDASWVKILTVAYFFGSFLNHNLFLAIHELSHNLAFTTPSYNRWLGIFANLPIGV 125

Query: 123 PMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLFLK 182
           PMSITFQKYHLEHHRFQGVDG+DMD+PSQ EAH V N +SK++WV+FQLFFYALRPLFLK
Sbjct: 126 PMSITFQKYHLEHHRFQGVDGIDMDIPSQAEAHAVKNTLSKSVWVVFQLFFYALRPLFLK 185

Query: 183 PKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNP 242
           PKPPG WEF N IIQIALD +MVYF+ WKS AYLILSTFVGGGMHPMAGHFISEHYVFNP
Sbjct: 186 PKPPGLWEFTNLIIQIALDASMVYFFGWKSLAYLILSTFVGGGMHPMAGHFISEHYVFNP 245

Query: 243 KQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQVIY 302
            QETYSYYGPLNL+TW VGYHNEHHDFPRIPG++LYKV+EIAPEYYN L SYKSWSQVIY
Sbjct: 246 DQETYSYYGPLNLMTWHVGYHNEHHDFPRIPGTRLYKVREIAPEYYNNLKSYKSWSQVIY 305

Query: 303 MYIMDKTVGPFSRMKRMPRKSE 325
           MYIMD+TVGPFSRMKR   K +
Sbjct: 306 MYIMDQTVGPFSRMKRKAPKKD 327

BLAST of PI0000760 vs. ExPASy Swiss-Prot
Match: Q9ZPH4 (Sphingolipid delta(4)-desaturase DES1-like OS=Arabidopsis thaliana OX=3702 GN=At4g04930 PE=2 SV=1)

HSP 1 Score: 572.4 bits (1474), Expect = 3.3e-162
Identity = 266/324 (82.10%), Postives = 293/324 (90.43%), Query Frame = 0

Query: 1   MQGGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ 60
           +   EE  +GVMATDFFWSYTDEPHASRRRQILS YPQIR+LFGPDPWAF KI++VV+LQ
Sbjct: 9   ISSNEEEREGVMATDFFWSYTDEPHASRRRQILSCYPQIRQLFGPDPWAFLKITLVVILQ 68

Query: 61  LWTATTLHRADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI 120
           L TA  LH + WLKIL+IAYFFGSFLNHNLFLAIHELSHNLAFSTP +NR LGIFANLPI
Sbjct: 69  LSTAAILHNSGWLKILSIAYFFGSFLNHNLFLAIHELSHNLAFSTPVYNRCLGIFANLPI 128

Query: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180
           GVPMS+TFQKYHLEHHRFQGVDG+DMDVP+ TEAHLVTN+ +K IWV  QLFFYALRP+F
Sbjct: 129 GVPMSVTFQKYHLEHHRFQGVDGIDMDVPTYTEAHLVTNIFAKTIWVFLQLFFYALRPIF 188

Query: 181 LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240
           +KPKPPG+WEFINF+IQI LDV++V F+ W+SFAYLILSTFVGGGMHPMAGHFISEHYVF
Sbjct: 189 IKPKPPGYWEFINFLIQIVLDVSVVLFFGWRSFAYLILSTFVGGGMHPMAGHFISEHYVF 248

Query: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300
           NP QETYSYYGPLNLLTWSVGYHNEHHDFPRIPG+KL+ VKEIA EYY  L+SYKSWSQV
Sbjct: 249 NPNQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGNKLHLVKEIAGEYYEGLESYKSWSQV 308

Query: 301 IYMYIMDKTVGPFSRMKRMPRKSE 325
           IYMYIMD TVGP+SRMKR   KS+
Sbjct: 309 IYMYIMDTTVGPYSRMKRKLSKSD 332

BLAST of PI0000760 vs. ExPASy Swiss-Prot
Match: Q6UQ04 (Sphingolipid delta(4)-desaturase DES1 OS=Microtus fortis OX=100897 GN=DEGS1 PE=2 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 4.1e-96
Identity = 160/316 (50.63%), Postives = 221/316 (69.94%), Query Frame = 0

Query: 11  VMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQLWTATTLHRA 70
           V   +F W YTD+PH  RRR+IL++YP+I+ L  PDP   + I+ + L+QL +   +   
Sbjct: 5   VSREEFEWVYTDQPHTDRRREILAKYPEIKSLMKPDPNLIWIITAMFLVQLISLYLVKDL 64

Query: 71  DWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFS--TPDHNRWLGIFANLPIGVPMSITF 130
           DW  ++  +Y FGS LNH++ LAIHE+SHN  F       NRW GIFANLPIGVP S++F
Sbjct: 65  DWKWVIFWSYVFGSCLNHSMTLAIHEISHNFPFGHHKAVWNRWFGIFANLPIGVPYSVSF 124

Query: 131 QKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLFLKPKPPGH 190
           ++YH++HHR+ G DG+D+D+P+  E         K +WVI Q  FYALRPLF+ PKP  +
Sbjct: 125 KRYHMDHHRYLGADGIDVDIPTDFEGWFFCTTFRKLVWVILQPLFYALRPLFINPKPITY 184

Query: 191 WEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYS 250
            E IN + QI LD+ + Y +  KS  Y++ ++ +G G+HP++GHFI+EHY+F    ETYS
Sbjct: 185 LEIINTVTQIILDIIVYYVFGIKSLVYMLAASLLGLGLHPISGHFIAEHYMFLKGHETYS 244

Query: 251 YYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQVIYMYIMDK 310
           YYGPLNLLT++VGYHNEHHDFP +PG  L  V++IA EYY++L  Y SW +V+Y ++MD 
Sbjct: 245 YYGPLNLLTFNVGYHNEHHDFPNVPGKNLPLVRKIAAEYYDKLPQYNSWIKVLYDFVMDD 304

Query: 311 TVGPFSRMKRMPRKSE 325
           T+ P+SRMKR P+ SE
Sbjct: 305 TISPYSRMKRPPKGSE 320

BLAST of PI0000760 vs. ExPASy Swiss-Prot
Match: O15121 (Sphingolipid delta(4)-desaturase DES1 OS=Homo sapiens OX=9606 GN=DEGS1 PE=1 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 1.3e-94
Identity = 159/310 (51.29%), Postives = 215/310 (69.35%), Query Frame = 0

Query: 11  VMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQLWTATTLHRA 70
           V   DF W YTD+PHA RRR+IL++YP+I+ L  PDP   + I ++VL QL     +   
Sbjct: 5   VSREDFEWVYTDQPHADRRREILAKYPEIKSLMKPDPNLIWIIIMMVLTQLGAFYIVKDL 64

Query: 71  DWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFST--PDHNRWLGIFANLPIGVPMSITF 130
           DW  ++  AY FGS +NH++ LAIHE++HN AF       NRW G+FANLPIG+P SI+F
Sbjct: 65  DWKWVIFGAYAFGSCINHSMTLAIHEIAHNAAFGNCKAMWNRWFGMFANLPIGIPYSISF 124

Query: 131 QKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLFLKPKPPGH 190
           ++YH++HHR+ G DG+D+D+P+  E         K IWVI Q  FYA RPLF+ PKP  +
Sbjct: 125 KRYHMDHHRYLGADGVDVDIPTDFEGWFFCTAFRKFIWVILQPLFYAFRPLFINPKPITY 184

Query: 191 WEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYS 250
            E IN + Q+  D+ + YF   KS  Y++ ++ +G G+HP++GHFI+EHY+F    ETYS
Sbjct: 185 LEVINTVAQVTFDILIYYFLGIKSLVYMLAASLLGLGLHPISGHFIAEHYMFLKGHETYS 244

Query: 251 YYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQVIYMYIMDK 310
           YYGPLNLLT++VGYHNEHHDFP IPG  L  V++IA EYY+ L  Y SW +V+Y ++MD 
Sbjct: 245 YYGPLNLLTFNVGYHNEHHDFPNIPGKSLPLVRKIAAEYYDNLPHYNSWIKVLYDFVMDD 304

Query: 311 TVGPFSRMKR 319
           T+ P+SRMKR
Sbjct: 305 TISPYSRMKR 314

BLAST of PI0000760 vs. ExPASy Swiss-Prot
Match: Q5XIF5 (Sphingolipid delta(4)-desaturase DES1 OS=Rattus norvegicus OX=10116 GN=Degs1 PE=2 SV=1)

HSP 1 Score: 347.1 bits (889), Expect = 2.2e-94
Identity = 156/316 (49.37%), Postives = 222/316 (70.25%), Query Frame = 0

Query: 11  VMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQLWTATTLHRA 70
           V   +F W YTD+PHA+RR++IL++YP+I+ L  PDP   + ++ ++L+QL +   +   
Sbjct: 5   VSREEFEWVYTDQPHAARRQEILAKYPEIKSLMKPDPNLIWIVTSMLLVQLASFYLVKDL 64

Query: 71  DWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFS--TPDHNRWLGIFANLPIGVPMSITF 130
           DW  ++  +Y FGS LNH++ LAIHE+SHN  F       NRW G+FANL +GVP SI+F
Sbjct: 65  DWKWLMFWSYAFGSCLNHSMTLAIHEISHNFPFGHHKAMWNRWFGMFANLSLGVPYSISF 124

Query: 131 QKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLFLKPKPPGH 190
           ++YH++HHR+ G DG+D+D+P+  E       + K +WVI Q  FYA RPLF+ PKP  H
Sbjct: 125 KRYHMDHHRYLGADGIDVDIPTDFEGWFFCTTLRKLVWVILQPLFYAFRPLFINPKPITH 184

Query: 191 WEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVFNPKQETYS 250
            E IN +IQ+  DV + Y +  KS  Y++ ++ +G G+HP++GHFI+EHY+F    ETYS
Sbjct: 185 LEVINTVIQVTFDVLVYYVFGVKSLVYMLAASLLGLGLHPISGHFIAEHYMFLKGHETYS 244

Query: 251 YYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQVIYMYIMDK 310
           YYGPLNLLT++VGYHNEHHDFP +PG  L  V++IA EYY+ L  Y SW +V+Y ++MD 
Sbjct: 245 YYGPLNLLTFNVGYHNEHHDFPNVPGKNLPLVRKIASEYYDNLPHYNSWIRVLYDFVMDD 304

Query: 311 TVGPFSRMKRMPRKSE 325
           T+ P+SRMKR P+ +E
Sbjct: 305 TISPYSRMKRPPKGNE 320

BLAST of PI0000760 vs. ExPASy TrEMBL
Match: A0A0A0LSB9 (Sphingolipid delta(4)-desaturase DES1-like OS=Cucumis sativus OX=3659 GN=Csa_1G073750 PE=3 SV=1)

HSP 1 Score: 679.5 bits (1752), Expect = 7.0e-192
Identity = 320/324 (98.77%), Postives = 322/324 (99.38%), Query Frame = 0

Query: 1   MQGGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ 60
           M GGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ
Sbjct: 1   MPGGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ 60

Query: 61  LWTATTLHRADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI 120
           LWTATTLH ADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPD+N+WLGIFANLPI
Sbjct: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI 120

Query: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180
           GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF
Sbjct: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180

Query: 181 LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240
           LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF
Sbjct: 181 LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240

Query: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300
           NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV
Sbjct: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300

Query: 301 IYMYIMDKTVGPFSRMKRMPRKSE 325
           IYMYIMDKTVGPFSRMKRMPRKSE
Sbjct: 301 IYMYIMDKTVGPFSRMKRMPRKSE 324

BLAST of PI0000760 vs. ExPASy TrEMBL
Match: A0A5D3DSS5 (Sphingolipid delta(4)-desaturase DES1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00680 PE=3 SV=1)

HSP 1 Score: 676.8 bits (1745), Expect = 4.5e-191
Identity = 318/324 (98.15%), Postives = 322/324 (99.38%), Query Frame = 0

Query: 1   MQGGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ 60
           MQGG ERDDGVMATDFFWSYTDEPHASRRRQILS+YPQIRELFGPDPWAFFKIS+VVLLQ
Sbjct: 1   MQGGAERDDGVMATDFFWSYTDEPHASRRRQILSKYPQIRELFGPDPWAFFKISMVVLLQ 60

Query: 61  LWTATTLHRADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI 120
           LWTATTLH ADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPD+N+WLGIFANLPI
Sbjct: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI 120

Query: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180
           GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF
Sbjct: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180

Query: 181 LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240
           LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF
Sbjct: 181 LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240

Query: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300
           NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV
Sbjct: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300

Query: 301 IYMYIMDKTVGPFSRMKRMPRKSE 325
           IYMYIMDKTVGPFSRMKRMPRKSE
Sbjct: 301 IYMYIMDKTVGPFSRMKRMPRKSE 324

BLAST of PI0000760 vs. ExPASy TrEMBL
Match: A0A1S3B5L3 (Sphingolipid delta(4)-desaturase DES1-like OS=Cucumis melo OX=3656 GN=LOC103486276 PE=3 SV=1)

HSP 1 Score: 676.8 bits (1745), Expect = 4.5e-191
Identity = 318/324 (98.15%), Postives = 322/324 (99.38%), Query Frame = 0

Query: 1   MQGGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ 60
           MQGG ERDDGVMATDFFWSYTDEPHASRRRQILS+YPQIRELFGPDPWAFFKIS+VVLLQ
Sbjct: 1   MQGGAERDDGVMATDFFWSYTDEPHASRRRQILSKYPQIRELFGPDPWAFFKISMVVLLQ 60

Query: 61  LWTATTLHRADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI 120
           LWTATTLH ADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPD+N+WLGIFANLPI
Sbjct: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI 120

Query: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180
           GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF
Sbjct: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180

Query: 181 LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240
           LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF
Sbjct: 181 LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240

Query: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300
           NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV
Sbjct: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300

Query: 301 IYMYIMDKTVGPFSRMKRMPRKSE 325
           IYMYIMDKTVGPFSRMKRMPRKSE
Sbjct: 301 IYMYIMDKTVGPFSRMKRMPRKSE 324

BLAST of PI0000760 vs. ExPASy TrEMBL
Match: A0A6J1EFX3 (Sphingolipid delta(4)-desaturase DES1-like OS=Cucurbita moschata OX=3662 GN=LOC111433967 PE=3 SV=1)

HSP 1 Score: 657.5 bits (1695), Expect = 2.8e-185
Identity = 306/324 (94.44%), Postives = 316/324 (97.53%), Query Frame = 0

Query: 1   MQGGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ 60
           M+GGEERD+GVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ
Sbjct: 1   MRGGEERDEGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ 60

Query: 61  LWTATTLHRADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI 120
           LWTATTLH A+WLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI
Sbjct: 61  LWTATTLHNAEWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI 120

Query: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180
           GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEA LVTNV+SKAIWV+ QLFFYALRPLF
Sbjct: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEARLVTNVISKAIWVVLQLFFYALRPLF 180

Query: 181 LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240
           LKPKPPG WEFINF IQI LDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF
Sbjct: 181 LKPKPPGRWEFINFFIQITLDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240

Query: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300
           NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPG+KLYKVKEIAPEYYNELDSYKSWSQV
Sbjct: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGNKLYKVKEIAPEYYNELDSYKSWSQV 300

Query: 301 IYMYIMDKTVGPFSRMKRMPRKSE 325
           IYMYIMD++VGPFSRMKR+ +K E
Sbjct: 301 IYMYIMDRSVGPFSRMKRLSKKPE 324

BLAST of PI0000760 vs. ExPASy TrEMBL
Match: A0A6J1GTF2 (Sphingolipid delta(4)-desaturase DES1-like OS=Cucurbita moschata OX=3662 GN=LOC111457308 PE=3 SV=1)

HSP 1 Score: 657.5 bits (1695), Expect = 2.8e-185
Identity = 306/324 (94.44%), Postives = 315/324 (97.22%), Query Frame = 0

Query: 1   MQGGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ 60
           M  GEERD+GVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKI+VVVLLQ
Sbjct: 1   MPRGEERDEGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKIAVVVLLQ 60

Query: 61  LWTATTLHRADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI 120
           LWTATTLH ADWLKILA+AYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI
Sbjct: 61  LWTATTLHSADWLKILAVAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI 120

Query: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180
           GVPMSITFQKYHLEHHRFQGVDGMDMDVPS TEA LVTN+VSKAIWV+FQLFFYALRPLF
Sbjct: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSTTEARLVTNIVSKAIWVVFQLFFYALRPLF 180

Query: 181 LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240
           LKPKPPGHWEFINFIIQI LD  MVYFWSWK+FAYLILSTFVGGGMHPMAGHFISEHYVF
Sbjct: 181 LKPKPPGHWEFINFIIQIGLDFTMVYFWSWKTFAYLILSTFVGGGMHPMAGHFISEHYVF 240

Query: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300
           NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPE+YNELDSYKSWSQV
Sbjct: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEFYNELDSYKSWSQV 300

Query: 301 IYMYIMDKTVGPFSRMKRMPRKSE 325
           IYMYIMD+TVGPFSRMKRM +KSE
Sbjct: 301 IYMYIMDRTVGPFSRMKRMAKKSE 324

BLAST of PI0000760 vs. NCBI nr
Match: XP_004146595.1 (sphingolipid delta(4)-desaturase DES1-like [Cucumis sativus] >KGN64668.1 hypothetical protein Csa_014335 [Cucumis sativus])

HSP 1 Score: 679.5 bits (1752), Expect = 1.4e-191
Identity = 320/324 (98.77%), Postives = 322/324 (99.38%), Query Frame = 0

Query: 1   MQGGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ 60
           M GGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ
Sbjct: 1   MPGGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ 60

Query: 61  LWTATTLHRADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI 120
           LWTATTLH ADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPD+N+WLGIFANLPI
Sbjct: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI 120

Query: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180
           GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF
Sbjct: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180

Query: 181 LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240
           LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF
Sbjct: 181 LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240

Query: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300
           NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV
Sbjct: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300

Query: 301 IYMYIMDKTVGPFSRMKRMPRKSE 325
           IYMYIMDKTVGPFSRMKRMPRKSE
Sbjct: 301 IYMYIMDKTVGPFSRMKRMPRKSE 324

BLAST of PI0000760 vs. NCBI nr
Match: XP_008442400.1 (PREDICTED: sphingolipid delta(4)-desaturase DES1-like [Cucumis melo] >KAA0056978.1 sphingolipid delta(4)-desaturase DES1-like [Cucumis melo var. makuwa] >TYK26405.1 sphingolipid delta(4)-desaturase DES1-like [Cucumis melo var. makuwa])

HSP 1 Score: 676.8 bits (1745), Expect = 9.3e-191
Identity = 318/324 (98.15%), Postives = 322/324 (99.38%), Query Frame = 0

Query: 1   MQGGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ 60
           MQGG ERDDGVMATDFFWSYTDEPHASRRRQILS+YPQIRELFGPDPWAFFKIS+VVLLQ
Sbjct: 1   MQGGAERDDGVMATDFFWSYTDEPHASRRRQILSKYPQIRELFGPDPWAFFKISMVVLLQ 60

Query: 61  LWTATTLHRADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI 120
           LWTATTLH ADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPD+N+WLGIFANLPI
Sbjct: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNKWLGIFANLPI 120

Query: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180
           GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF
Sbjct: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180

Query: 181 LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240
           LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF
Sbjct: 181 LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240

Query: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300
           NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV
Sbjct: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300

Query: 301 IYMYIMDKTVGPFSRMKRMPRKSE 325
           IYMYIMDKTVGPFSRMKRMPRKSE
Sbjct: 301 IYMYIMDKTVGPFSRMKRMPRKSE 324

BLAST of PI0000760 vs. NCBI nr
Match: XP_038893930.1 (sphingolipid delta(4)-desaturase DES1-like [Benincasa hispida])

HSP 1 Score: 671.8 bits (1732), Expect = 3.0e-189
Identity = 316/324 (97.53%), Postives = 320/324 (98.77%), Query Frame = 0

Query: 1   MQGGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ 60
           MQGGEERD+GVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ
Sbjct: 1   MQGGEERDEGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ 60

Query: 61  LWTATTLHRADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI 120
           LWTATTLH ADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPD+NRWLGIFANLPI
Sbjct: 61  LWTATTLHSADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDYNRWLGIFANLPI 120

Query: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180
           GVPMSITFQKYHLEHHRFQGVDGMDMDVPS TEAHLVTNVVSKAIWV+FQLFFYALRPLF
Sbjct: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSLTEAHLVTNVVSKAIWVVFQLFFYALRPLF 180

Query: 181 LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240
           LKPKPPG WEFINFIIQIALDVAMVY WSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF
Sbjct: 181 LKPKPPGQWEFINFIIQIALDVAMVYLWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240

Query: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300
           NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV
Sbjct: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300

Query: 301 IYMYIMDKTVGPFSRMKRMPRKSE 325
           IYMYIMD+TVGPFSRMKRMPRKSE
Sbjct: 301 IYMYIMDRTVGPFSRMKRMPRKSE 324

BLAST of PI0000760 vs. NCBI nr
Match: KAG7012427.1 (Sphingolipid delta(4)-desaturase DES1-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 657.9 bits (1696), Expect = 4.5e-185
Identity = 306/324 (94.44%), Postives = 315/324 (97.22%), Query Frame = 0

Query: 1   MQGGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ 60
           M  GEERD+GVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKI+VVVLLQ
Sbjct: 1   MPRGEERDEGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKIAVVVLLQ 60

Query: 61  LWTATTLHRADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI 120
           LWTATTLH ADWLKILA+AYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI
Sbjct: 61  LWTATTLHSADWLKILAVAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI 120

Query: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180
           GVPMSITFQKYHLEHHRFQGVDGMDMDVPS TEA LVTN+VSKAIWV+FQLFFYALRPLF
Sbjct: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSMTEARLVTNIVSKAIWVVFQLFFYALRPLF 180

Query: 181 LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240
           LKPKPPGHWEFINFIIQI LD  MVYFWSWK+FAYLILSTFVGGGMHPMAGHFISEHYVF
Sbjct: 181 LKPKPPGHWEFINFIIQIGLDFTMVYFWSWKTFAYLILSTFVGGGMHPMAGHFISEHYVF 240

Query: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300
           NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPE+YNELDSYKSWSQV
Sbjct: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEFYNELDSYKSWSQV 300

Query: 301 IYMYIMDKTVGPFSRMKRMPRKSE 325
           IYMYIMD+TVGPFSRMKRM +KSE
Sbjct: 301 IYMYIMDRTVGPFSRMKRMAKKSE 324

BLAST of PI0000760 vs. NCBI nr
Match: XP_022927006.1 (sphingolipid delta(4)-desaturase DES1-like [Cucurbita moschata] >XP_022927007.1 sphingolipid delta(4)-desaturase DES1-like [Cucurbita moschata] >KAG7019623.1 Sphingolipid delta(4)-desaturase DES1-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 657.5 bits (1695), Expect = 5.9e-185
Identity = 306/324 (94.44%), Postives = 316/324 (97.53%), Query Frame = 0

Query: 1   MQGGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ 60
           M+GGEERD+GVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ
Sbjct: 1   MRGGEERDEGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ 60

Query: 61  LWTATTLHRADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI 120
           LWTATTLH A+WLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI
Sbjct: 61  LWTATTLHNAEWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI 120

Query: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180
           GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEA LVTNV+SKAIWV+ QLFFYALRPLF
Sbjct: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEARLVTNVISKAIWVVLQLFFYALRPLF 180

Query: 181 LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240
           LKPKPPG WEFINF IQI LDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF
Sbjct: 181 LKPKPPGRWEFINFFIQITLDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240

Query: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300
           NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPG+KLYKVKEIAPEYYNELDSYKSWSQV
Sbjct: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGNKLYKVKEIAPEYYNELDSYKSWSQV 300

Query: 301 IYMYIMDKTVGPFSRMKRMPRKSE 325
           IYMYIMD++VGPFSRMKR+ +K E
Sbjct: 301 IYMYIMDRSVGPFSRMKRLSKKPE 324

BLAST of PI0000760 vs. TAIR 10
Match: AT4G04930.1 (fatty acid desaturase family protein )

HSP 1 Score: 572.4 bits (1474), Expect = 2.3e-163
Identity = 266/324 (82.10%), Postives = 293/324 (90.43%), Query Frame = 0

Query: 1   MQGGEERDDGVMATDFFWSYTDEPHASRRRQILSQYPQIRELFGPDPWAFFKISVVVLLQ 60
           +   EE  +GVMATDFFWSYTDEPHASRRRQILS YPQIR+LFGPDPWAF KI++VV+LQ
Sbjct: 9   ISSNEEEREGVMATDFFWSYTDEPHASRRRQILSCYPQIRQLFGPDPWAFLKITLVVILQ 68

Query: 61  LWTATTLHRADWLKILAIAYFFGSFLNHNLFLAIHELSHNLAFSTPDHNRWLGIFANLPI 120
           L TA  LH + WLKIL+IAYFFGSFLNHNLFLAIHELSHNLAFSTP +NR LGIFANLPI
Sbjct: 69  LSTAAILHNSGWLKILSIAYFFGSFLNHNLFLAIHELSHNLAFSTPVYNRCLGIFANLPI 128

Query: 121 GVPMSITFQKYHLEHHRFQGVDGMDMDVPSQTEAHLVTNVVSKAIWVIFQLFFYALRPLF 180
           GVPMS+TFQKYHLEHHRFQGVDG+DMDVP+ TEAHLVTN+ +K IWV  QLFFYALRP+F
Sbjct: 129 GVPMSVTFQKYHLEHHRFQGVDGIDMDVPTYTEAHLVTNIFAKTIWVFLQLFFYALRPIF 188

Query: 181 LKPKPPGHWEFINFIIQIALDVAMVYFWSWKSFAYLILSTFVGGGMHPMAGHFISEHYVF 240
           +KPKPPG+WEFINF+IQI LDV++V F+ W+SFAYLILSTFVGGGMHPMAGHFISEHYVF
Sbjct: 189 IKPKPPGYWEFINFLIQIVLDVSVVLFFGWRSFAYLILSTFVGGGMHPMAGHFISEHYVF 248

Query: 241 NPKQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGSKLYKVKEIAPEYYNELDSYKSWSQV 300
           NP QETYSYYGPLNLLTWSVGYHNEHHDFPRIPG+KL+ VKEIA EYY  L+SYKSWSQV
Sbjct: 249 NPNQETYSYYGPLNLLTWSVGYHNEHHDFPRIPGNKLHLVKEIAGEYYEGLESYKSWSQV 308

Query: 301 IYMYIMDKTVGPFSRMKRMPRKSE 325
           IYMYIMD TVGP+SRMKR   KS+
Sbjct: 309 IYMYIMDTTVGPYSRMKRKLSKSD 332

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6H5U38.6e-16381.68Sphingolipid delta(4)-desaturase DES1-like OS=Oryza sativa subsp. japonica OX=39... [more]
Q9ZPH43.3e-16282.10Sphingolipid delta(4)-desaturase DES1-like OS=Arabidopsis thaliana OX=3702 GN=At... [more]
Q6UQ044.1e-9650.63Sphingolipid delta(4)-desaturase DES1 OS=Microtus fortis OX=100897 GN=DEGS1 PE=2... [more]
O151211.3e-9451.29Sphingolipid delta(4)-desaturase DES1 OS=Homo sapiens OX=9606 GN=DEGS1 PE=1 SV=1[more]
Q5XIF52.2e-9449.37Sphingolipid delta(4)-desaturase DES1 OS=Rattus norvegicus OX=10116 GN=Degs1 PE=... [more]
Match NameE-valueIdentityDescription
A0A0A0LSB97.0e-19298.77Sphingolipid delta(4)-desaturase DES1-like OS=Cucumis sativus OX=3659 GN=Csa_1G0... [more]
A0A5D3DSS54.5e-19198.15Sphingolipid delta(4)-desaturase DES1-like OS=Cucumis melo var. makuwa OX=119469... [more]
A0A1S3B5L34.5e-19198.15Sphingolipid delta(4)-desaturase DES1-like OS=Cucumis melo OX=3656 GN=LOC1034862... [more]
A0A6J1EFX32.8e-18594.44Sphingolipid delta(4)-desaturase DES1-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1GTF22.8e-18594.44Sphingolipid delta(4)-desaturase DES1-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
Match NameE-valueIdentityDescription
XP_004146595.11.4e-19198.77sphingolipid delta(4)-desaturase DES1-like [Cucumis sativus] >KGN64668.1 hypothe... [more]
XP_008442400.19.3e-19198.15PREDICTED: sphingolipid delta(4)-desaturase DES1-like [Cucumis melo] >KAA0056978... [more]
XP_038893930.13.0e-18997.53sphingolipid delta(4)-desaturase DES1-like [Benincasa hispida][more]
KAG7012427.14.5e-18594.44Sphingolipid delta(4)-desaturase DES1-like protein [Cucurbita argyrosperma subsp... [more]
XP_022927006.15.9e-18594.44sphingolipid delta(4)-desaturase DES1-like [Cucurbita moschata] >XP_022927007.1 ... [more]
Match NameE-valueIdentityDescription
AT4G04930.12.3e-16382.10fatty acid desaturase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013866Sphingolipid delta4-desaturase, N-terminalSMARTSM01269Lipid_DES_2coord: 11..49
e-value: 7.2E-21
score: 85.3
IPR013866Sphingolipid delta4-desaturase, N-terminalPFAMPF08557Lipid_DEScoord: 14..48
e-value: 6.7E-20
score: 70.3
IPR011388Sphingolipid delta4-desaturasePIRSFPIRSF017228Sphnglp_dlt4_descoord: 4..324
e-value: 4.6E-149
score: 493.9
IPR011388Sphingolipid delta4-desaturaseCDDcd03508Delta4-sphingolipid-FADS-likecoord: 32..318
e-value: 1.82931E-170
score: 472.899
IPR005804Fatty acid desaturase domainPFAMPF00487FA_desaturasecoord: 72..290
e-value: 2.4E-21
score: 76.8
NoneNo IPR availablePANTHERPTHR12879:SF17SPHINGOLIPID DELTA(4)-DESATURASE DES1-LIKEcoord: 5..322
NoneNo IPR availablePANTHERPTHR12879SPHINGOLIPID DELTA 4 DESATURASE/C-4 HYDROXYLASE PROTEIN DES2coord: 5..322

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0000760.1PI0000760.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046513 ceramide biosynthetic process
biological_process GO:0006629 lipid metabolic process
biological_process GO:0030148 sphingolipid biosynthetic process
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0042284 sphingolipid delta-4 desaturase activity