PI0000661 (gene) Melon (PI 482460) v1

Overview


Name	PI0000661
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	Non-structural maintenance of chromosomes element 4
Location	chr02: 3986902 .. 3989257 (-)
RNA-Seq Expression	PI0000661
Synteny	PI0000661

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGGCCTGTCAAACGCGAACCCACCGTTAGCACCCGCAGTAATCGAAATAGAGGCGGCGACGGCGGTGGCGGCGCTGTTGATGGTGCGAGTTTAAGGGATAGTAAGCGGGAGAGGGTTGCTCGGGAGAGAGAGAACTCCGGCGAGGAGAATATGACTGAAGAAGAAGTGGGTCAGCAGGATTTGACGAAGAGGCGGGTCCTTCGCTCTCGGTACCTCGCGGTTATTCATGAGATTAGTGGTATGATGAACCTTTGCATTTTGGATTTTGAGATGTGGGTTTGATTTGTTTGTGTGTGTAATTGGTTTTTGTTTGTTTCTTGAATTTGAAGAAAGAAGAGAGGACTTGTCGAAGGATTTGGATAAGTTTAATGTTATCATCAATGAAGTGGAAAAGTTGCACGAGCAAGGTATTTTTGTAGTCTTTTCTTATTGTTGTCCTTGTCTCTAATTCTAATGCAAATTTAAAGCGACGATAGTGTGCATTTTTGCCCTTTATCCTCTGTATTCTTATATTCATACAAGTTTTTATGTCAGTTTGAATAGTTGTTAATTTTCCTTTTGATGTTGTTATCTACGTGACATGGCTGTGTTTTCGTTTGGGTTTCAGTTCAGAAGCCAAGGGAACAAGTTGCAGATGCAGAAGCTTTGCAAGATATTGCAAATTCCCTTGTGACCTCTATTAGGTCCCAGTCAAATGAAGGTGTTACTCCATCAGATTTTGTTAGTTGTCTACTCAGAGAGTTTGCTGACATTAACGGTAGTATAAAATCTGAAGAACGAGACCTGGTTTCTGTCAATTGGAAAGATATTGGACTCAGTGTTTCATCTATTTTCATGAATGGCTATGGATGCCGCACCATGTAAGATTCATGGATTCCTTTTTCCCACTATCCTGATTCTGAAGTGTCTTGAATTGAGGTTCTTTGTCAACTATATAACTTTCTTTCTTGTATGTGTTTGTGTTTTTTTTTTCTCAAGGCTTGGACCTATGAGCAATCAGTTGAAGCAGCGAAAGACTAATAATGTTTCCAGGAAACGTTCCAGGCCTACTGAAAGTTCTAGACCTGCAGAGGTTAGACCAAAAAGATTTTAATAGTTTAAAGTATTTTGGATTTAGGATCGAGTAATTTGTCACTCTGTTTTGAGCTTTTGTTATTCTGTTGCTTTGACCTGAAAAAGGCTTTGCTTTCAAAATTCCTAGCACGGTTAACTTTTTCAGCACTCTGTTAGTTTTGAGTCTAACAGAAGTACAGTTATTTCTTTCTTGGCTGGAGATACATCATCGAACTTGCTAATGCTTCATCTTGACATTGACAAAGTTTGTTTGGAATCACTCAATGCTTGCGTTCATGACGTTCTTCACTTAACATTATTAACCTTTTTTTTTGGTTTAAGTGGTCTGTGAATTAGATTGTGAAATAGAAGTTTGTTTCTTCAGAATTTGACATTCTACCATCCCGTTGATGATTGTTTCAATTCACACCGCAGGTTGAAGATAATGGGTCTGAACCAAAAACAGATACAGATAAGAACATGGGCATCATGTTTGGAATTTTGAGGAGGAATAAAACCGTTAAACTAGAACATTTGATATTGAATAGAAAGTCTTTTGCACAGACAGTGGAGAATTTATTTGCTCTATCCTTTCTGGTTAAGGATGGACGAGCTGAGATAACTATTGATAAAAAGGGTTCTCATTTTGTGTGTGAGTAAACATCTACAAACTAAACATTTTATGCTTTTGTTTGAAAACATTATGTTCTTTGCACAATGCTAACATTTCCTGCCAACATTTTCAGCACCGAAGAATGCCCCTGCTCATAGTGCCATTATGTCACATGAAGTTAATTACAGTCATTTTGTATTCAGATTCGATTACAAGGATTGGAAGGTACATATTTCGCATTCATATTTATGATACTAACTTCGCATTTTGCGGTGTCTAACATACATCAAATATTAAGAAAATCATTCTGCTCCCTTGATTTCGAGTGTATACAATATTGGATAGTGTTTCATGATATATGCAAAATGCTGTGTGCTATTTAGATCCTTGGATCCAATTGAGGAACACATACTTGTGATGCTTTATTTGCAGCCAATGGTGGACATGGTACCAGTTGGAGAGGAGCTGATGCCCCACAGGAACTTTCTGAACTCAATGCAGGTGTCCCAAGAAGAAGCGACGGCGGACGATTCTGAATCTGCAGGGTTACCTACCACACCAATTCGAAAGCTGTCAAGAAATCGAGGTTTAGTTATGCAATCTGTTGTTGAAGATTCTCCTGACAATGTCTCTCAATCAGGAGCAACGGCAATTTTGAGGGGCAAGAGAAAGCTTACTCAAGGTTAG

mRNA sequence

ATGAGGCCTGTCAAACGCGAACCCACCGTTAGCACCCGCAGTAATCGAAATAGAGGCGGCGACGGCGGTGGCGGCGCTGTTGATGGTGCGAGTTTAAGGGATAGTAAGCGGGAGAGGGTTGCTCGGGAGAGAGAGAACTCCGGCGAGGAGAATATGACTGAAGAAGAAGTGGGTCAGCAGGATTTGACGAAGAGGCGGGTCCTTCGCTCTCGGTACCTCGCGGTTATTCATGAGATTAGTGAAAGAAGAGAGGACTTGTCGAAGGATTTGGATAAGTTTAATGTTATCATCAATGAAGTGGAAAAGTTGCACGAGCAAGTTCAGAAGCCAAGGGAACAAGTTGCAGATGCAGAAGCTTTGCAAGATATTGCAAATTCCCTTGTGACCTCTATTAGGTCCCAGTCAAATGAAGGTGTTACTCCATCAGATTTTGTTAGTTGTCTACTCAGAGAGTTTGCTGACATTAACGGTAGTATAAAATCTGAAGAACGAGACCTGGTTTCTGTCAATTGGAAAGATATTGGACTCAGTGTTTCATCTATTTTCATGAATGGCTATGGATGCCGCACCATGCTTGGACCTATGAGCAATCAGTTGAAGCAGCGAAAGACTAATAATGTTTCCAGGAAACGTTCCAGGCCTACTGAAAGTTCTAGACCTGCAGAGGTTGAAGATAATGGGTCTGAACCAAAAACAGATACAGATAAGAACATGGGCATCATGTTTGGAATTTTGAGGAGGAATAAAACCGTTAAACTAGAACATTTGATATTGAATAGAAAGTCTTTTGCACAGACAGTGGAGAATTTATTTGCTCTATCCTTTCTGGTTAAGGATGGACGAGCTGAGATAACTATTGATAAAAAGGGTTCTCATTTTGTGTCACCGAAGAATGCCCCTGCTCATAGTGCCATTATGTCACATGAAGTTAATTACAGTCATTTTGTATTCAGATTCGATTACAAGGATTGGAAGCCAATGGTGGACATGGTACCAGTTGGAGAGGAGCTGATGCCCCACAGGAACTTTCTGAACTCAATGCAGGTGTCCCAAGAAGAAGCGACGGCGGACGATTCTGAATCTGCAGGGTTACCTACCACACCAATTCGAAAGCTGTCAAGAAATCGAGGTTTAGTTATGCAATCTGTTGTTGAAGATTCTCCTGACAATGTCTCTCAATCAGGAGCAACGGCAATTTTGAGGGGCAAGAGAAAGCTTACTCAAGGTTAG

Coding sequence (CDS)

ATGAGGCCTGTCAAACGCGAACCCACCGTTAGCACCCGCAGTAATCGAAATAGAGGCGGCGACGGCGGTGGCGGCGCTGTTGATGGTGCGAGTTTAAGGGATAGTAAGCGGGAGAGGGTTGCTCGGGAGAGAGAGAACTCCGGCGAGGAGAATATGACTGAAGAAGAAGTGGGTCAGCAGGATTTGACGAAGAGGCGGGTCCTTCGCTCTCGGTACCTCGCGGTTATTCATGAGATTAGTGAAAGAAGAGAGGACTTGTCGAAGGATTTGGATAAGTTTAATGTTATCATCAATGAAGTGGAAAAGTTGCACGAGCAAGTTCAGAAGCCAAGGGAACAAGTTGCAGATGCAGAAGCTTTGCAAGATATTGCAAATTCCCTTGTGACCTCTATTAGGTCCCAGTCAAATGAAGGTGTTACTCCATCAGATTTTGTTAGTTGTCTACTCAGAGAGTTTGCTGACATTAACGGTAGTATAAAATCTGAAGAACGAGACCTGGTTTCTGTCAATTGGAAAGATATTGGACTCAGTGTTTCATCTATTTTCATGAATGGCTATGGATGCCGCACCATGCTTGGACCTATGAGCAATCAGTTGAAGCAGCGAAAGACTAATAATGTTTCCAGGAAACGTTCCAGGCCTACTGAAAGTTCTAGACCTGCAGAGGTTGAAGATAATGGGTCTGAACCAAAAACAGATACAGATAAGAACATGGGCATCATGTTTGGAATTTTGAGGAGGAATAAAACCGTTAAACTAGAACATTTGATATTGAATAGAAAGTCTTTTGCACAGACAGTGGAGAATTTATTTGCTCTATCCTTTCTGGTTAAGGATGGACGAGCTGAGATAACTATTGATAAAAAGGGTTCTCATTTTGTGTCACCGAAGAATGCCCCTGCTCATAGTGCCATTATGTCACATGAAGTTAATTACAGTCATTTTGTATTCAGATTCGATTACAAGGATTGGAAGCCAATGGTGGACATGGTACCAGTTGGAGAGGAGCTGATGCCCCACAGGAACTTTCTGAACTCAATGCAGGTGTCCCAAGAAGAAGCGACGGCGGACGATTCTGAATCTGCAGGGTTACCTACCACACCAATTCGAAAGCTGTCAAGAAATCGAGGTTTAGTTATGCAATCTGTTGTTGAAGATTCTCCTGACAATGTCTCTCAATCAGGAGCAACGGCAATTTTGAGGGGCAAGAGAAAGCTTACTCAAGGTTAG

Protein sequence

MRPVKREPTVSTRSNRNRGGDGGGGAVDGASLRDSKRERVARERENSGEENMTEEEVGQQDLTKRRVLRSRYLAVIHEISERREDLSKDLDKFNVIINEVEKLHEQVQKPREQVADAEALQDIANSLVTSIRSQSNEGVTPSDFVSCLLREFADINGSIKSEERDLVSVNWKDIGLSVSSIFMNGYGCRTMLGPMSNQLKQRKTNNVSRKRSRPTESSRPAEVEDNGSEPKTDTDKNMGIMFGILRRNKTVKLEHLILNRKSFAQTVENLFALSFLVKDGRAEITIDKKGSHFVSPKNAPAHSAIMSHEVNYSHFVFRFDYKDWKPMVDMVPVGEELMPHRNFLNSMQVSQEEATADDSESAGLPTTPIRKLSRNRGLVMQSVVEDSPDNVSQSGATAILRGKRKLTQG

Homology

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BLAST of PI0000661 vs. ExPASy Swiss-Prot
Match: Q9C689 (Non-structural maintenance of chromosomes element 4 homolog A OS=Arabidopsis thaliana OX=3702 GN=NSE4A PE=2 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 3.0e-96
Identity = 203/383 (53.00%), Postives = 271/383 (70.76%), Query Frame = 0


Query: 31  SLRDSKRERVARERENSGEENMTEEEVGQQDLTKRRVLRSRYLAVIHEISERREDLSK-D 90
           S++  K+ +   +     E    +EE  +Q ++ RR+LRS+YL++ +EI++ ++DL K D
Sbjct: 27  SVKKEKQRKTEADSVRPDEPPPPQEE--EQGISDRRILRSKYLSLQNEINDCKDDLMKID 86

Query: 91  LDKFNVIINEVEKLHEQVQKPREQVADAEALQDIANSLVTSIRSQSNEGVTPSDFVSCLL 150
            DKF+ IIN VE LH+QV+KPREQ+ADAEAL DIAN+L++S++SQS  GV+P++FV+ L+
Sbjct: 87  SDKFSRIINAVENLHQQVRKPREQIADAEALLDIANTLMSSVKSQSAHGVSPAEFVNALI 146

Query: 151 REFADINGSIKSEERDLVSVNWKDIGLSVSSIFMNGYGCRTMLGPMSNQLKQRKTNNVSR 210
             F   +  I ++E   VS+ WKD+G +V S  +   GC TMLGPM  +LKQRK    +R
Sbjct: 147 SGFGQGSLGIDTDETAQVSLKWKDLGFAVCSTVLVSCGCSTMLGPMDTELKQRK-RAPNR 206

Query: 211 KRSRPTESSRPAEVEDNGSEPKTDTDKNMGIMFGILRRNKTVKLEHLILNRKSFAQTVEN 270
           KR++P E  RP EV+D+ SE KTDTDKNM IMF IL + K V+LE+L+LNR+SFAQTVEN
Sbjct: 207 KRTKPGEGVRPDEVDDSQSEEKTDTDKNMTIMFNILGKKKRVQLENLVLNRRSFAQTVEN 266

Query: 271 LFALSFLVKDGRAEITIDKKGSHFVSPKNAPAHSAIMSHEVNYSHFVFRFDYKDWKPMVD 330
           LFALSFL KDGR EI +DK GSHF  P+NAP  + +MS EV Y+HFVFR D+KDWK M +
Sbjct: 267 LFALSFLAKDGRVEIIVDKSGSHFAMPRNAPDANVVMSGEVIYNHFVFRLDFKDWKLMSE 326

Query: 331 MVPVGEELMPHRNFL---NSMQVSQEEATADDSESAGLPTTPIRKLSRNRGLVMQ--SVV 390
           MVP+GEELMPHR      +S   +   A+AD ++     TTPIRKLSRNRGLV+Q  +VV
Sbjct: 327 MVPLGEELMPHRQTAVASSSCPAASAPASADFTQDT--QTTPIRKLSRNRGLVVQEETVV 386

Query: 391 EDSPDNVSQSGATAILRGKRKLT 408
           ED+PD   + G     R KR+LT
Sbjct: 387 EDTPD---KEGDGTRRRCKRRLT 401

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BLAST of PI0000661 vs. ExPASy Swiss-Prot
Match: F4JET1 (Non-structural maintenance of chromosomes element 4 homolog B OS=Arabidopsis thaliana OX=3702 GN=NSE4B PE=2 SV=1)

HSP 1 Score: 320.1 bits (819), Expect = 3.7e-86
Identity = 173/342 (50.58%), Postives = 242/342 (70.76%), Query Frame = 0


Query: 32  LRDSKRERVARERENSGEENMTEEEVGQQDLTKRRVLRSRYLAVIHEISERREDLSK-DL 91
           ++  KR +         +  + EEE  +Q +  RR +RS+YLA+ H+I + ++DL+K D 
Sbjct: 31  VKKEKRSKTVIASLRPQQPPIQEEE--EQGIADRRAIRSQYLALTHKIKDAKDDLTKIDS 90

Query: 92  DKFNVIINEVEKLHEQVQKPREQVADAEALQDIANSLVTSIRSQS-NEGVTPSDFVSCLL 151
           +KFN IINEVE LH++V+KPREQ+ADAEAL D+ NS+V+S++SQS + GV+P++FV+ L+
Sbjct: 91  NKFNSIINEVENLHQKVRKPREQIADAEALLDLTNSVVSSVKSQSAHGGVSPAEFVNALI 150

Query: 152 REFADINGSIKSEERDLVSVNWKDIGLSVSSIFMNGYGCRTMLGPMSNQLKQRKTNNVSR 211
             F   +  I ++E   VS+ WKD+G +V S  +   GC TM+GPM +++KQRK+   +R
Sbjct: 151 NGFGKTSLRIDADENTQVSMKWKDLGFTVCSTVLVSCGCTTMMGPMYSEMKQRKSRVGNR 210

Query: 212 KRSRPTESSRPAEVEDNGSEPKTDTDKNMGIMFGILRRNKTVKLEHLILNRKSFAQTVEN 271
           KR++P    +P EV+D  +E K+DTD NM +MF ILR+NK VK+E+L+LNRKSFAQT EN
Sbjct: 211 KRTKPGAGVKPEEVDDTEAEKKSDTDNNMAVMFNILRKNKRVKIENLVLNRKSFAQTAEN 270

Query: 272 LFALSFLVKDGRAEITIDKKGSHFVSPKNAPAHSAIMSHEVNYSHFVFRFDYKDWKPMVD 331
           +FALSFLVKDGR EIT+D  GSHFV P+NAPA + ++S EV Y+HFV RFDYKDW+PM  
Sbjct: 271 MFALSFLVKDGRVEITVDNNGSHFVEPRNAPAANLVLSGEVAYNHFVLRFDYKDWEPMSK 330

Query: 332 MVPVGEELMPHRN---FLNSMQVS-------QEEATADDSES 362
           MV VGEELMP R      +S+++S       QEE   +DS +
Sbjct: 331 MVAVGEELMPLRETKVAQDSIELSRKQGSVIQEETVVEDSSN 370

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BLAST of PI0000661 vs. ExPASy Swiss-Prot
Match: Q2TBI1 (Non-structural maintenance of chromosomes element 4 homolog A OS=Bos taurus OX=9913 GN=NSMCE4A PE=2 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 1.7e-11
Identity = 70/318 (22.01%), Postives = 138/318 (43.40%), Query Frame = 0


Query: 43  ERENSGEENMTEEEVGQQ-DLTKRRVLRSRYLAVIHEISERREDLSKDLDKFNVIINEVE 102
           E  +SG+E +    + ++ D +  R +R +Y A+I+ + + RED+    DK   ++ E  
Sbjct: 59  EPSDSGDEMIDPASLEEETDPSLCRQIRHQYRALINSVQQNREDILNASDKLTEVLEEAN 118

Query: 103 KLHEQVQKPREQVADAEAL---QDIANSLVTSIRSQSNEGVTPSDFVSCLLREFA----D 162
            L   V + RE V DA  L    D+       +RS  N       +V  LL        +
Sbjct: 119 TLFNGVSRAREAVLDAHFLVLASDLGKEKAKQLRSDLN-SFDMLRYVETLLTHMGVNPLE 178

Query: 163 INGSIKSEERD----LVSVNWKDIGLSVSSIFMNGYGCRTMLGPMSNQL--------KQR 222
               I+ E+      +V  +WK  G +  + F   +    +LG +  +         + R
Sbjct: 179 AEELIRDEDSSDLEFIVYDSWKISGKTAENTFNKTHTFHFLLGSIQGECPVPKPRIERPR 238

Query: 223 KTNNVSRKRSRPTESSRPAEVEDNGSEPKTDTDKNMGIMFGILRRNKTVKLE--HLILNR 282
           K   +  +++ P +  R  E     +E   + ++ +G++    + +    +     +++ 
Sbjct: 239 KVRMIEEQQAMPAQLKRMEESHQEATE--KEVERILGLLQTYFQEDPDTPMSFFDFVVDP 298

Query: 283 KSFAQTVENLFALSFLVKDGRAEITIDKKGSHFVSPKNAPAHSAIMSHEVNYSHFVFRFD 339
            SF +TVEN+F +SF+++DG A I +D+     + P N    S   +   N +  +    
Sbjct: 299 HSFPRTVENIFHVSFIIRDGFARIRLDRDRLPVIEPVNINEESGGNTQIRNQA--IIALS 358

[Close]

BLAST of PI0000661 vs. ExPASy Swiss-Prot
Match: Q6BDR8 (Non-structural maintenance of chromosome element 4 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=nse4 PE=1 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 2.5e-10
Identity = 65/283 (22.97%), Postives = 127/283 (44.88%), Query Frame = 0


Query: 65  RRVLRSRYLAVIHEISERREDL-SKDLDKFNVIINEVEKLHEQVQKPREQVADAEALQDI 124
           +R LR RY  +I+++ E R +L  ++ +     I     L   V  P E   DA  L   
Sbjct: 6   KRDLRKRYRNLINKVQESRLELVDEENNNLYETITTANDLFSSVDAPTEATLDALLLTKT 65

Query: 125 ANSLVTSIRSQSNEGVTPS---DFVSCLLREFADINGSIKSEER-DLVSVNWKDIGLSVS 184
            +  + SI+++      P    +  +  +++F +   S  +  R   +   W  +G   S
Sbjct: 66  VD--LASIKARQLHIGRPKFNIELFTKNIKQFLNYPTSHSNVTRIQEIDTAWSRLGKLAS 125

Query: 185 SIFMNGYGCRTMLGPMSNQLKQRKTNNVSRKRSRPTESSRPAEV-EDNGSEPKTDTDKNM 244
           +          M+GP+S + K+R      R +  P   ++P  + E N +  + +T KN+
Sbjct: 126 NCEKQPASLNLMVGPLSFRKKERNIQRRERLQKAPNVLTQPTMLNERNITTQENNTTKNV 185

Query: 245 GIMFGILRRNKTVKLEHLILNRKSFAQTVENLFALSFLVKDGRAEITIDKKGSHFVSPKN 304
             +  +L+ ++ V     I N +S+ QTVENLF +SFL K+G+A +  ++ G   +  + 
Sbjct: 186 LHISRLLQAHQPVNFLKFITNPQSYPQTVENLFYVSFLFKEGKAALVENESGILMLETRI 245

Query: 305 APAHSAIMSHEVNYSHFVFRFDYKDWKPMVDMVPVGEELMPHR 342
            P    +++ E+     V       ++ ++    + E ++P R
Sbjct: 246 PPTDDQVVAGEIRNIQLVLDMTMDLYENIIKEYNIKESIIPTR 286

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BLAST of PI0000661 vs. ExPASy Swiss-Prot
Match: Q9NXX6 (Non-structural maintenance of chromosomes element 4 homolog A OS=Homo sapiens OX=9606 GN=NSMCE4A PE=1 SV=2)

HSP 1 Score: 67.0 bits (162), Expect = 5.6e-10
Identity = 82/356 (23.03%), Postives = 149/356 (41.85%), Query Frame = 0


Query: 15  NRNRGGDGGGGAVDGASLRDSKRE-RVARER--------ENSGEENMTEEEV-GQQDLTK 74
           +R R        +   S R S RE R A ER         +SG+E M    +  + D   
Sbjct: 22  DRTRSRSRSRSPLSPRSRRGSARERREAPERPSLEDTEPSDSGDEMMDPASLEAEADQGL 81

Query: 75  RRVLRSRYLAVIHEISERREDLSKDLDKFNVIINEVEKLHEQVQKPREQVADAEALQDIA 134
            R +R +Y A+I+ + + RED+    DK   ++ E   L  +V + RE V DA  L  +A
Sbjct: 82  CRQIRHQYRALINSVQQNREDILNAGDKLTEVLEEANTLFNEVSRAREAVLDAHFLV-LA 141

Query: 135 NSLVTSIRSQSNEGVTPSD---FVSCLLREFA----DINGSIKSEERD----LVSVNWKD 194
           + L      Q    ++  D   +V  LL        +    I+ E+      +V  +WK 
Sbjct: 142 SDLGKEKAKQLRSDLSSFDMLRYVETLLTHMGVNPLEAEELIRDEDSPDFEFIVYDSWKI 201

Query: 195 IGLSVSSIFMNGYGCRTMLG--------PMSNQLKQRKTNNVSRKRSRPTESSRPAEVED 254
            G +  + F   +    +LG        P     + RK   +  +R+ P +  R  E   
Sbjct: 202 TGRTAENTFNKTHTFHFLLGSIYGECPVPKPRVDRPRKVPVIQEERAMPAQLRRMEESHQ 261

Query: 255 NGSEPKTDTDKNMGIMFGILRRNKTVKLE--HLILNRKSFAQTVENLFALSFLVKDGRAE 314
             +E   + ++ +G++    R +    +     +++  SF +TVEN+F +SF+++DG A 
Sbjct: 262 EATE--KEVERILGLLQTYFREDPDTPMSFFDFVVDPHSFPRTVENIFHVSFIIRDGFAR 321

Query: 315 ITIDKKGSHFVSPKNAPAHSAIMSHEVNY-SHFVFRFDYKDWKPMVDMVPVGEELM 339
           I +D+     + P +    +    H     +  +    Y+DW+ +V    + E ++
Sbjct: 322 IRLDQDRLPVIEPVSINEENEGFEHNTQVRNQGIIALSYRDWEEIVKTFEISEPVI 374

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BLAST of PI0000661 vs. ExPASy TrEMBL
Match: A0A1S3BQS4 (Non-structural maintenance of chromosomes element 4 OS=Cucumis melo OX=3656 GN=LOC103492497 PE=3 SV=1)

HSP 1 Score: 750.0 bits (1935), Expect = 5.3e-213
Identity = 392/409 (95.84%), Postives = 400/409 (97.80%), Query Frame = 0


Query: 1   MRPVKREPTVSTRSNRNRGGDGGGGAVDGASLRDSKRERVARERENSGEENMTEEEVGQQ 60
           MRPVKREPTVSTRS RNRGGDGGGGAVDGASLRDSKRERVARERENSGEENM+ EEVGQQ
Sbjct: 1   MRPVKREPTVSTRSKRNRGGDGGGGAVDGASLRDSKRERVARERENSGEENMS-EEVGQQ 60

Query: 61  DLTKRRVLRSRYLAVIHEISERREDLSKDLDKFNVIINEVEKLHEQVQKPREQVADAEAL 120
           DLTKRRVLRSRYLAVIHEISERREDLSKDLDKFNVIINEVEKLHEQVQKPREQVADAEAL
Sbjct: 61  DLTKRRVLRSRYLAVIHEISERREDLSKDLDKFNVIINEVEKLHEQVQKPREQVADAEAL 120

Query: 121 QDIANSLVTSIRSQSNEGVTPSDFVSCLLREFADINGSIKSEERDLVSVNWKDIGLSVSS 180
           QDIANSLVTSIRSQSNEGVTPSDFVSCLLREFADINGSI SEE++LVSVNWKDIGLSVSS
Sbjct: 121 QDIANSLVTSIRSQSNEGVTPSDFVSCLLREFADINGSINSEEQELVSVNWKDIGLSVSS 180

Query: 181 IFMNGYGCRTMLGPMSNQLKQRKTNNVSRKRSRPTESSRPAEVEDNGSEPKTDTDKNMGI 240
           IFMNGYGCRTMLGPMSNQLKQRKTNNV RKRS+PTESSRPAEVEDNGSEPKTDTDKNMGI
Sbjct: 181 IFMNGYGCRTMLGPMSNQLKQRKTNNVPRKRSKPTESSRPAEVEDNGSEPKTDTDKNMGI 240

Query: 241 MFGILRRNKTVKLEHLILNRKSFAQTVENLFALSFLVKDGRAEITIDKKGSHFVSPKNAP 300
           MFGILRRNKTVKLEHLILNRKSFAQTVENLFALSFLVKDGRAEITIDK GSHFVSPKNAP
Sbjct: 241 MFGILRRNKTVKLEHLILNRKSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAP 300

Query: 301 AHSAIMSHEVNYSHFVFRFDYKDWKPMVDMVPVGEELMPHRNFLNSMQVSQEEATADDSE 360
           AHSAIMSHEVNYSHFVFRFDYKDWKPMV+MVPVGEELMPHRNFLNS+ VS+EEATADDSE
Sbjct: 301 AHSAIMSHEVNYSHFVFRFDYKDWKPMVNMVPVGEELMPHRNFLNSVPVSEEEATADDSE 360

Query: 361 SAGLPTTPIRKLSRNRGLVMQSVVEDSPDNVSQSGATAILRGKRKLTQG 410
           SA LPTTPIRKLSRNRGLVMQSVVEDSPDN SQSG TAILRGKRKL+QG
Sbjct: 361 SASLPTTPIRKLSRNRGLVMQSVVEDSPDNGSQSGPTAILRGKRKLSQG 408

[Close]

BLAST of PI0000661 vs. ExPASy TrEMBL
Match: A0A0A0LZJ0 (Non-structural maintenance of chromosomes element 4 OS=Cucumis sativus OX=3659 GN=Csa_1G605680 PE=3 SV=1)

HSP 1 Score: 746.1 bits (1925), Expect = 7.7e-212
Identity = 394/413 (95.40%), Postives = 398/413 (96.37%), Query Frame = 0


Query: 1   MRPVKREPTVSTRSNRNRGGD----GGGGAVDGASLRDSKRERVARERENSGEENMTEEE 60
           MRPVKREPTVSTRSNRNRGGD    GGGGAVDGASLRDSKRERVARERENSGEENM  EE
Sbjct: 1   MRPVKREPTVSTRSNRNRGGDGGGGGGGGAVDGASLRDSKRERVARERENSGEENM-NEE 60

Query: 61  VGQQDLTKRRVLRSRYLAVIHEISERREDLSKDLDKFNVIINEVEKLHEQVQKPREQVAD 120
           VGQQDLTKRRVLRSRYLAVIHEISERREDLSKDLDKFNVIINEVEKLHEQVQKPREQVAD
Sbjct: 61  VGQQDLTKRRVLRSRYLAVIHEISERREDLSKDLDKFNVIINEVEKLHEQVQKPREQVAD 120

Query: 121 AEALQDIANSLVTSIRSQSNEGVTPSDFVSCLLREFADINGSIKSEERDLVSVNWKDIGL 180
           AEALQDIANSLVTSIRSQSNEGVTPSDFVSCLLREFADINGSI SEE+DLVSVNWKDIGL
Sbjct: 121 AEALQDIANSLVTSIRSQSNEGVTPSDFVSCLLREFADINGSINSEEQDLVSVNWKDIGL 180

Query: 181 SVSSIFMNGYGCRTMLGPMSNQLKQRKTNNVSRKRSRPTESSRPAEVEDNGSEPKTDTDK 240
           SVSSIFMNGYGCRTMLGPMSNQLKQRKTNNV RKR RPTESSRPAEVEDNGSEPKTDTDK
Sbjct: 181 SVSSIFMNGYGCRTMLGPMSNQLKQRKTNNVPRKRVRPTESSRPAEVEDNGSEPKTDTDK 240

Query: 241 NMGIMFGILRRNKTVKLEHLILNRKSFAQTVENLFALSFLVKDGRAEITIDKKGSHFVSP 300
           NMGIMFGILRRNKTVKLEHLILNRKSF QTVENLFALSFLVKDGRAEITIDK GSHFVSP
Sbjct: 241 NMGIMFGILRRNKTVKLEHLILNRKSFGQTVENLFALSFLVKDGRAEITIDKNGSHFVSP 300

Query: 301 KNAPAHSAIMSHEVNYSHFVFRFDYKDWKPMVDMVPVGEELMPHRNFLNSMQVSQEEATA 360
           KNAPAHSAIMSHEVNYSHFVFRFDYKDWKPMVDMVPVGEELMPHR FLNS+QVSQEE  A
Sbjct: 301 KNAPAHSAIMSHEVNYSHFVFRFDYKDWKPMVDMVPVGEELMPHRTFLNSVQVSQEEVAA 360

Query: 361 DDSESAGLPTTPIRKLSRNRGLVMQSVVEDSPDNVSQSGATAILRGKRKLTQG 410
           D+SESAGLPTTPIRKLSRNRGLVMQSVVEDSPDN SQSGATAILRGKRKL+QG
Sbjct: 361 DNSESAGLPTTPIRKLSRNRGLVMQSVVEDSPDNGSQSGATAILRGKRKLSQG 412

[Close]

BLAST of PI0000661 vs. ExPASy TrEMBL
Match: A0A5A7TLP8 (Non-structural maintenance of chromosomes element 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1121G00010 PE=3 SV=1)

HSP 1 Score: 720.3 bits (1858), Expect = 4.5e-204
Identity = 381/409 (93.15%), Postives = 389/409 (95.11%), Query Frame = 0


Query: 1   MRPVKREPTVSTRSNRNRGGDGGGGAVDGASLRDSKRERVARERENSGEENMTEEEVGQQ 60
           MRPVKREPTVSTRS RNRGGDGGGGAVDGASLRDSKRERVARERENSGEENM+ EEVGQQ
Sbjct: 1   MRPVKREPTVSTRSKRNRGGDGGGGAVDGASLRDSKRERVARERENSGEENMS-EEVGQQ 60

Query: 61  DLTKRRVLRSRYLAVIHEISERREDLSKDLDKFNVIINEVEKLHEQVQKPREQVADAEAL 120
           DLTKRRVLRS           RREDLSKDLDKFNVIINEVEKLHEQVQKPREQVADAEAL
Sbjct: 61  DLTKRRVLRS-----------RREDLSKDLDKFNVIINEVEKLHEQVQKPREQVADAEAL 120

Query: 121 QDIANSLVTSIRSQSNEGVTPSDFVSCLLREFADINGSIKSEERDLVSVNWKDIGLSVSS 180
           QDIANSLVTSIRSQSNEGVTPSDFVSCLLREFADINGSI SEE++LVSVNWKDIGLSVSS
Sbjct: 121 QDIANSLVTSIRSQSNEGVTPSDFVSCLLREFADINGSINSEEQELVSVNWKDIGLSVSS 180

Query: 181 IFMNGYGCRTMLGPMSNQLKQRKTNNVSRKRSRPTESSRPAEVEDNGSEPKTDTDKNMGI 240
           IFMNGYGCRTMLGPMSNQLKQRKTNNV RKRS+PTESSRPAEVEDNGSEPKTDTDKNMGI
Sbjct: 181 IFMNGYGCRTMLGPMSNQLKQRKTNNVPRKRSKPTESSRPAEVEDNGSEPKTDTDKNMGI 240

Query: 241 MFGILRRNKTVKLEHLILNRKSFAQTVENLFALSFLVKDGRAEITIDKKGSHFVSPKNAP 300
           MFGILRRNKTVKLEHLILNRKSFAQTVENLFALSFLVKDGRAEITIDK GSHFVSPKNAP
Sbjct: 241 MFGILRRNKTVKLEHLILNRKSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAP 300

Query: 301 AHSAIMSHEVNYSHFVFRFDYKDWKPMVDMVPVGEELMPHRNFLNSMQVSQEEATADDSE 360
           AHSAIMSHEVNYSHFVFRFDYKDWKPMV+MVPVGEELMPHRNFLNS+ VS+EEATADDSE
Sbjct: 301 AHSAIMSHEVNYSHFVFRFDYKDWKPMVNMVPVGEELMPHRNFLNSVPVSEEEATADDSE 360

Query: 361 SAGLPTTPIRKLSRNRGLVMQSVVEDSPDNVSQSGATAILRGKRKLTQG 410
           SA LPTTPIRKLSRNRGLVMQSVVEDSPDN SQSG TAILRGKRKL+QG
Sbjct: 361 SASLPTTPIRKLSRNRGLVMQSVVEDSPDNGSQSGPTAILRGKRKLSQG 397

[Close]

BLAST of PI0000661 vs. ExPASy TrEMBL
Match: A0A6J1HBJ2 (Non-structural maintenance of chromosomes element 4 OS=Cucurbita moschata OX=3662 GN=LOC111461359 PE=3 SV=1)

HSP 1 Score: 670.6 bits (1729), Expect = 4.1e-189
Identity = 356/410 (86.83%), Postives = 375/410 (91.46%), Query Frame = 0


Query: 1   MRPVKREPTVSTRSNRNRGGDGGGGAVDGASLRDSKRER-VARERENSGEENMTEEEVGQ 60
           MRPVKREP  S+RSNR+R   GG  A D ASLRD KRE+ V+RERE SG +N TEEE GQ
Sbjct: 1   MRPVKREPAASSRSNRSR---GGAVAFDAASLRDVKREKVVSREREKSGVDNTTEEEEGQ 60

Query: 61  QDLTKRRVLRSRYLAVIHEISERREDLSKDLDKFNVIINEVEKLHEQVQKPREQVADAEA 120
           Q+L+KRRVLRSRYLAVIHEISERREDLSKDLDKFNVIINEVEKLHEQVQKPREQVADAEA
Sbjct: 61  QELSKRRVLRSRYLAVIHEISERREDLSKDLDKFNVIINEVEKLHEQVQKPREQVADAEA 120

Query: 121 LQDIANSLVTSIRSQSNEGVTPSDFVSCLLREFADINGSIKSEERDLVSVNWKDIGLSVS 180
           LQDIA+SLVTSIRSQSNEGVTPS+FVSCLLREFADING+I SEE DLVSV+WKDIGL VS
Sbjct: 121 LQDIASSLVTSIRSQSNEGVTPSNFVSCLLREFADINGNITSEENDLVSVSWKDIGLGVS 180

Query: 181 SIFMNGYGCRTMLGPMSNQLKQRKTNNVSRKRSRPTESSRPAEVEDNGSEPKTDTDKNMG 240
           SIFMNG+GCRTMLGPMSNQLKQRKT N+SRKRSRPTES RPAEV DNGSE KTDTDKNMG
Sbjct: 181 SIFMNGHGCRTMLGPMSNQLKQRKTTNISRKRSRPTESYRPAEVGDNGSEEKTDTDKNMG 240

Query: 241 IMFGILRRNKTVKLEHLILNRKSFAQTVENLFALSFLVKDGRAEITIDKKGSHFVSPKNA 300
           IMFGILRRNKTVKLEHLILNRKSFAQTVENLFALSFLVKDGRA ITIDK GSHFVSPKNA
Sbjct: 241 IMFGILRRNKTVKLEHLILNRKSFAQTVENLFALSFLVKDGRAAITIDKNGSHFVSPKNA 300

Query: 301 PAHSAIMSHEVNYSHFVFRFDYKDWKPMVDMVPVGEELMPHRNFLNSMQVSQEEATADDS 360
           PAHSAIMSHEVNYSHFVFRFD+KDWKPMVDMVPVG+ELMPHRN LNS QV Q EA+ DDS
Sbjct: 301 PAHSAIMSHEVNYSHFVFRFDFKDWKPMVDMVPVGDELMPHRNILNSAQVPQAEASTDDS 360

Query: 361 ESAGLPTTPIRKLSRNRGLVMQSVVEDSPDNVSQSGATAILRGKRKLTQG 410
           E+A LPTTPIRKLSRNRGLVMQSVVEDSPDN + S AT+ILR KRKL QG
Sbjct: 361 EAAALPTTPIRKLSRNRGLVMQSVVEDSPDNGAHSRATSILRCKRKLNQG 407

[Close]

BLAST of PI0000661 vs. ExPASy TrEMBL
Match: A0A6J1JLE2 (Non-structural maintenance of chromosomes element 4 OS=Cucurbita maxima OX=3661 GN=LOC111485470 PE=3 SV=1)

HSP 1 Score: 666.8 bits (1719), Expect = 5.9e-188
Identity = 353/409 (86.31%), Postives = 372/409 (90.95%), Query Frame = 0


Query: 1   MRPVKREPTVSTRSNRNRGGDGGGGAVDGASLRDSKRERVARERENSGEENMTEEEVGQQ 60
           MRPVKREP  S+R+NR+R   GG  AVD ASLRD KRERVA ERE SG +N T EE GQQ
Sbjct: 1   MRPVKREPASSSRNNRSR---GGSVAVDAASLRDVKRERVAWEREKSGVDNTTVEEEGQQ 60

Query: 61  DLTKRRVLRSRYLAVIHEISERREDLSKDLDKFNVIINEVEKLHEQVQKPREQVADAEAL 120
           +L+KRRVLRSRYLAVIHEISERREDLSKDLDKFNVIINEVEKLHEQV KPREQVADAEAL
Sbjct: 61  ELSKRRVLRSRYLAVIHEISERREDLSKDLDKFNVIINEVEKLHEQVHKPREQVADAEAL 120

Query: 121 QDIANSLVTSIRSQSNEGVTPSDFVSCLLREFADINGSIKSEERDLVSVNWKDIGLSVSS 180
           QDIA+SLVTSI SQSNEGVTPSDFVSCLLREFADING+I  EE +LVSV+WKDIGL VSS
Sbjct: 121 QDIASSLVTSIMSQSNEGVTPSDFVSCLLREFADINGNITCEENNLVSVSWKDIGLGVSS 180

Query: 181 IFMNGYGCRTMLGPMSNQLKQRKTNNVSRKRSRPTESSRPAEVEDNGSEPKTDTDKNMGI 240
           IFMN +GCRTMLGPMSNQLKQRKT N+SRKRSRPTES RPAEVEDNGSE KTDTDKNMGI
Sbjct: 181 IFMNCHGCRTMLGPMSNQLKQRKTTNISRKRSRPTESYRPAEVEDNGSEEKTDTDKNMGI 240

Query: 241 MFGILRRNKTVKLEHLILNRKSFAQTVENLFALSFLVKDGRAEITIDKKGSHFVSPKNAP 300
           MFGILRRNKTVKLEHLILNRKSFAQTVENLFALSFLVKDGRA ITIDK GSHFVSPKNAP
Sbjct: 241 MFGILRRNKTVKLEHLILNRKSFAQTVENLFALSFLVKDGRAAITIDKNGSHFVSPKNAP 300

Query: 301 AHSAIMSHEVNYSHFVFRFDYKDWKPMVDMVPVGEELMPHRNFLNSMQVSQEEATADDSE 360
           AHSAIMSHEVNYSHF+FRFD+ DWKPMVDMVPVG+ELMPHRN LNS QV Q EA+ DDSE
Sbjct: 301 AHSAIMSHEVNYSHFIFRFDFMDWKPMVDMVPVGDELMPHRNILNSAQVPQAEASTDDSE 360

Query: 361 SAGLPTTPIRKLSRNRGLVMQSVVEDSPDNVSQSGATAILRGKRKLTQG 410
           +A LPTTPIRKLSRNRGLVMQSVVEDSPDN + SGATAILR KRKL+QG
Sbjct: 361 AAALPTTPIRKLSRNRGLVMQSVVEDSPDNGAHSGATAILRCKRKLSQG 406

[Close]

BLAST of PI0000661 vs. NCBI nr
Match: XP_008451119.1 (PREDICTED: non-structural maintenance of chromosomes element 4 homolog A [Cucumis melo])

HSP 1 Score: 750.0 bits (1935), Expect = 1.1e-212
Identity = 392/409 (95.84%), Postives = 400/409 (97.80%), Query Frame = 0


Query: 1   MRPVKREPTVSTRSNRNRGGDGGGGAVDGASLRDSKRERVARERENSGEENMTEEEVGQQ 60
           MRPVKREPTVSTRS RNRGGDGGGGAVDGASLRDSKRERVARERENSGEENM+ EEVGQQ
Sbjct: 1   MRPVKREPTVSTRSKRNRGGDGGGGAVDGASLRDSKRERVARERENSGEENMS-EEVGQQ 60

Query: 61  DLTKRRVLRSRYLAVIHEISERREDLSKDLDKFNVIINEVEKLHEQVQKPREQVADAEAL 120
           DLTKRRVLRSRYLAVIHEISERREDLSKDLDKFNVIINEVEKLHEQVQKPREQVADAEAL
Sbjct: 61  DLTKRRVLRSRYLAVIHEISERREDLSKDLDKFNVIINEVEKLHEQVQKPREQVADAEAL 120

Query: 121 QDIANSLVTSIRSQSNEGVTPSDFVSCLLREFADINGSIKSEERDLVSVNWKDIGLSVSS 180
           QDIANSLVTSIRSQSNEGVTPSDFVSCLLREFADINGSI SEE++LVSVNWKDIGLSVSS
Sbjct: 121 QDIANSLVTSIRSQSNEGVTPSDFVSCLLREFADINGSINSEEQELVSVNWKDIGLSVSS 180

Query: 181 IFMNGYGCRTMLGPMSNQLKQRKTNNVSRKRSRPTESSRPAEVEDNGSEPKTDTDKNMGI 240
           IFMNGYGCRTMLGPMSNQLKQRKTNNV RKRS+PTESSRPAEVEDNGSEPKTDTDKNMGI
Sbjct: 181 IFMNGYGCRTMLGPMSNQLKQRKTNNVPRKRSKPTESSRPAEVEDNGSEPKTDTDKNMGI 240

Query: 241 MFGILRRNKTVKLEHLILNRKSFAQTVENLFALSFLVKDGRAEITIDKKGSHFVSPKNAP 300
           MFGILRRNKTVKLEHLILNRKSFAQTVENLFALSFLVKDGRAEITIDK GSHFVSPKNAP
Sbjct: 241 MFGILRRNKTVKLEHLILNRKSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAP 300

Query: 301 AHSAIMSHEVNYSHFVFRFDYKDWKPMVDMVPVGEELMPHRNFLNSMQVSQEEATADDSE 360
           AHSAIMSHEVNYSHFVFRFDYKDWKPMV+MVPVGEELMPHRNFLNS+ VS+EEATADDSE
Sbjct: 301 AHSAIMSHEVNYSHFVFRFDYKDWKPMVNMVPVGEELMPHRNFLNSVPVSEEEATADDSE 360

Query: 361 SAGLPTTPIRKLSRNRGLVMQSVVEDSPDNVSQSGATAILRGKRKLTQG 410
           SA LPTTPIRKLSRNRGLVMQSVVEDSPDN SQSG TAILRGKRKL+QG
Sbjct: 361 SASLPTTPIRKLSRNRGLVMQSVVEDSPDNGSQSGPTAILRGKRKLSQG 408

[Close]

BLAST of PI0000661 vs. NCBI nr
Match: XP_004149035.1 (non-structural maintenance of chromosomes element 4 homolog A [Cucumis sativus])

HSP 1 Score: 746.1 bits (1925), Expect = 1.6e-211
Identity = 394/413 (95.40%), Postives = 398/413 (96.37%), Query Frame = 0


Query: 1   MRPVKREPTVSTRSNRNRGGD----GGGGAVDGASLRDSKRERVARERENSGEENMTEEE 60
           MRPVKREPTVSTRSNRNRGGD    GGGGAVDGASLRDSKRERVARERENSGEENM  EE
Sbjct: 1   MRPVKREPTVSTRSNRNRGGDGGGGGGGGAVDGASLRDSKRERVARERENSGEENM-NEE 60

Query: 61  VGQQDLTKRRVLRSRYLAVIHEISERREDLSKDLDKFNVIINEVEKLHEQVQKPREQVAD 120
           VGQQDLTKRRVLRSRYLAVIHEISERREDLSKDLDKFNVIINEVEKLHEQVQKPREQVAD
Sbjct: 61  VGQQDLTKRRVLRSRYLAVIHEISERREDLSKDLDKFNVIINEVEKLHEQVQKPREQVAD 120

Query: 121 AEALQDIANSLVTSIRSQSNEGVTPSDFVSCLLREFADINGSIKSEERDLVSVNWKDIGL 180
           AEALQDIANSLVTSIRSQSNEGVTPSDFVSCLLREFADINGSI SEE+DLVSVNWKDIGL
Sbjct: 121 AEALQDIANSLVTSIRSQSNEGVTPSDFVSCLLREFADINGSINSEEQDLVSVNWKDIGL 180

Query: 181 SVSSIFMNGYGCRTMLGPMSNQLKQRKTNNVSRKRSRPTESSRPAEVEDNGSEPKTDTDK 240
           SVSSIFMNGYGCRTMLGPMSNQLKQRKTNNV RKR RPTESSRPAEVEDNGSEPKTDTDK
Sbjct: 181 SVSSIFMNGYGCRTMLGPMSNQLKQRKTNNVPRKRVRPTESSRPAEVEDNGSEPKTDTDK 240

Query: 241 NMGIMFGILRRNKTVKLEHLILNRKSFAQTVENLFALSFLVKDGRAEITIDKKGSHFVSP 300
           NMGIMFGILRRNKTVKLEHLILNRKSF QTVENLFALSFLVKDGRAEITIDK GSHFVSP
Sbjct: 241 NMGIMFGILRRNKTVKLEHLILNRKSFGQTVENLFALSFLVKDGRAEITIDKNGSHFVSP 300

Query: 301 KNAPAHSAIMSHEVNYSHFVFRFDYKDWKPMVDMVPVGEELMPHRNFLNSMQVSQEEATA 360
           KNAPAHSAIMSHEVNYSHFVFRFDYKDWKPMVDMVPVGEELMPHR FLNS+QVSQEE  A
Sbjct: 301 KNAPAHSAIMSHEVNYSHFVFRFDYKDWKPMVDMVPVGEELMPHRTFLNSVQVSQEEVAA 360

Query: 361 DDSESAGLPTTPIRKLSRNRGLVMQSVVEDSPDNVSQSGATAILRGKRKLTQG 410
           D+SESAGLPTTPIRKLSRNRGLVMQSVVEDSPDN SQSGATAILRGKRKL+QG
Sbjct: 361 DNSESAGLPTTPIRKLSRNRGLVMQSVVEDSPDNGSQSGATAILRGKRKLSQG 412

[Close]

BLAST of PI0000661 vs. NCBI nr
Match: XP_038879817.1 (non-structural maintenance of chromosomes element 4 homolog A isoform X1 [Benincasa hispida])

HSP 1 Score: 727.2 bits (1876), Expect = 7.6e-206
Identity = 380/409 (92.91%), Postives = 390/409 (95.35%), Query Frame = 0


Query: 1   MRPVKREPTVSTRSNRNRGGDGGGGAVDGASLRDSKRERVARERENSGEENMTEEEVGQQ 60
           MRPVKREPT S RSNR+RGG G GG VD ASL+D KRERVARERENSGE+N TEEEVGQQ
Sbjct: 1   MRPVKREPTASNRSNRSRGGGGDGGTVDAASLKDIKRERVARERENSGEDNTTEEEVGQQ 60

Query: 61  DLTKRRVLRSRYLAVIHEISERREDLSKDLDKFNVIINEVEKLHEQVQKPREQVADAEAL 120
           DLTKRRVLRSRYLAVIHEISERREDLSKD DKFNVIINEVEKLHEQVQKPREQVADAE L
Sbjct: 61  DLTKRRVLRSRYLAVIHEISERREDLSKDPDKFNVIINEVEKLHEQVQKPREQVADAEVL 120

Query: 121 QDIANSLVTSIRSQSNEGVTPSDFVSCLLREFADINGSIKSEERDLVSVNWKDIGLSVSS 180
           QDIANSLVTSIRSQSNEGVTPSDFVSCLLREFADIN S+KSEE DLVSV+WKDIGLSVSS
Sbjct: 121 QDIANSLVTSIRSQSNEGVTPSDFVSCLLREFADINSSMKSEEHDLVSVSWKDIGLSVSS 180

Query: 181 IFMNGYGCRTMLGPMSNQLKQRKTNNVSRKRSRPTESSRPAEVEDNGSEPKTDTDKNMGI 240
           IFMNG+GCRTMLGPMSNQLKQRKTNNVSRKRSRPTESSRPAEVED GSE KTDTDKNMGI
Sbjct: 181 IFMNGHGCRTMLGPMSNQLKQRKTNNVSRKRSRPTESSRPAEVEDTGSEAKTDTDKNMGI 240

Query: 241 MFGILRRNKTVKLEHLILNRKSFAQTVENLFALSFLVKDGRAEITIDKKGSHFVSPKNAP 300
           MFGILRRNKTVKLEHLILNRKSFAQTVENLFALSFLVKDGRAEITIDK GSHFVSPKNAP
Sbjct: 241 MFGILRRNKTVKLEHLILNRKSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAP 300

Query: 301 AHSAIMSHEVNYSHFVFRFDYKDWKPMVDMVPVGEELMPHRNFLNSMQVSQEEATADDSE 360
           AHSAIMSHEVNYSHFVFRFDYKDWKPMVDMVPVGEELMPHRNFLNS+QVS+ E TADDSE
Sbjct: 301 AHSAIMSHEVNYSHFVFRFDYKDWKPMVDMVPVGEELMPHRNFLNSVQVSEAEETADDSE 360

Query: 361 SAGLPTTPIRKLSRNRGLVMQSVVEDSPDNVSQSGATAILRGKRKLTQG 410
           +AGLPTTPIRKLSRNRGLVMQSVVEDSPDN SQSGATAILR KRKL+QG
Sbjct: 361 AAGLPTTPIRKLSRNRGLVMQSVVEDSPDNGSQSGATAILRCKRKLSQG 409

[Close]

BLAST of PI0000661 vs. NCBI nr
Match: XP_038879818.1 (non-structural maintenance of chromosomes element 4 homolog A isoform X2 [Benincasa hispida])

HSP 1 Score: 727.2 bits (1876), Expect = 7.6e-206
Identity = 380/409 (92.91%), Postives = 390/409 (95.35%), Query Frame = 0


Query: 1   MRPVKREPTVSTRSNRNRGGDGGGGAVDGASLRDSKRERVARERENSGEENMTEEEVGQQ 60
           MRPVKREPT S RSNR+RGG G GG VD ASL+D KRERVARERENSGE+N TEEEVGQQ
Sbjct: 1   MRPVKREPTASNRSNRSRGGGGDGGTVDAASLKDIKRERVARERENSGEDNTTEEEVGQQ 60

Query: 61  DLTKRRVLRSRYLAVIHEISERREDLSKDLDKFNVIINEVEKLHEQVQKPREQVADAEAL 120
           DLTKRRVLRSRYLAVIHEISERREDLSKD DKFNVIINEVEKLHEQVQKPREQVADAE L
Sbjct: 61  DLTKRRVLRSRYLAVIHEISERREDLSKDPDKFNVIINEVEKLHEQVQKPREQVADAEVL 120

Query: 121 QDIANSLVTSIRSQSNEGVTPSDFVSCLLREFADINGSIKSEERDLVSVNWKDIGLSVSS 180
           QDIANSLVTSIRSQSNEGVTPSDFVSCLLREFADIN S+KSEE DLVSV+WKDIGLSVSS
Sbjct: 121 QDIANSLVTSIRSQSNEGVTPSDFVSCLLREFADINSSMKSEEHDLVSVSWKDIGLSVSS 180

Query: 181 IFMNGYGCRTMLGPMSNQLKQRKTNNVSRKRSRPTESSRPAEVEDNGSEPKTDTDKNMGI 240
           IFMNG+GCRTMLGPMSNQLKQRKTNNVSRKRSRPTESSRPAEVED GSE KTDTDKNMGI
Sbjct: 181 IFMNGHGCRTMLGPMSNQLKQRKTNNVSRKRSRPTESSRPAEVEDTGSEAKTDTDKNMGI 240

Query: 241 MFGILRRNKTVKLEHLILNRKSFAQTVENLFALSFLVKDGRAEITIDKKGSHFVSPKNAP 300
           MFGILRRNKTVKLEHLILNRKSFAQTVENLFALSFLVKDGRAEITIDK GSHFVSPKNAP
Sbjct: 241 MFGILRRNKTVKLEHLILNRKSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAP 300

Query: 301 AHSAIMSHEVNYSHFVFRFDYKDWKPMVDMVPVGEELMPHRNFLNSMQVSQEEATADDSE 360
           AHSAIMSHEVNYSHFVFRFDYKDWKPMVDMVPVGEELMPHRNFLNS+QVS+ E TADDSE
Sbjct: 301 AHSAIMSHEVNYSHFVFRFDYKDWKPMVDMVPVGEELMPHRNFLNSVQVSEAEETADDSE 360

Query: 361 SAGLPTTPIRKLSRNRGLVMQSVVEDSPDNVSQSGATAILRGKRKLTQG 410
           +AGLPTTPIRKLSRNRGLVMQSVVEDSPDN SQSGATAILR KRKL+QG
Sbjct: 361 AAGLPTTPIRKLSRNRGLVMQSVVEDSPDNGSQSGATAILRCKRKLSQG 409

[Close]

BLAST of PI0000661 vs. NCBI nr
Match: KAA0042269.1 (non-structural maintenance of chromosomes element 4-like protein A [Cucumis melo var. makuwa] >TYK14223.1 non-structural maintenance of chromosomes element 4-like protein A [Cucumis melo var. makuwa])

HSP 1 Score: 720.3 bits (1858), Expect = 9.3e-204
Identity = 381/409 (93.15%), Postives = 389/409 (95.11%), Query Frame = 0


Query: 1   MRPVKREPTVSTRSNRNRGGDGGGGAVDGASLRDSKRERVARERENSGEENMTEEEVGQQ 60
           MRPVKREPTVSTRS RNRGGDGGGGAVDGASLRDSKRERVARERENSGEENM+ EEVGQQ
Sbjct: 1   MRPVKREPTVSTRSKRNRGGDGGGGAVDGASLRDSKRERVARERENSGEENMS-EEVGQQ 60

Query: 61  DLTKRRVLRSRYLAVIHEISERREDLSKDLDKFNVIINEVEKLHEQVQKPREQVADAEAL 120
           DLTKRRVLRS           RREDLSKDLDKFNVIINEVEKLHEQVQKPREQVADAEAL
Sbjct: 61  DLTKRRVLRS-----------RREDLSKDLDKFNVIINEVEKLHEQVQKPREQVADAEAL 120

Query: 121 QDIANSLVTSIRSQSNEGVTPSDFVSCLLREFADINGSIKSEERDLVSVNWKDIGLSVSS 180
           QDIANSLVTSIRSQSNEGVTPSDFVSCLLREFADINGSI SEE++LVSVNWKDIGLSVSS
Sbjct: 121 QDIANSLVTSIRSQSNEGVTPSDFVSCLLREFADINGSINSEEQELVSVNWKDIGLSVSS 180

Query: 181 IFMNGYGCRTMLGPMSNQLKQRKTNNVSRKRSRPTESSRPAEVEDNGSEPKTDTDKNMGI 240
           IFMNGYGCRTMLGPMSNQLKQRKTNNV RKRS+PTESSRPAEVEDNGSEPKTDTDKNMGI
Sbjct: 181 IFMNGYGCRTMLGPMSNQLKQRKTNNVPRKRSKPTESSRPAEVEDNGSEPKTDTDKNMGI 240

Query: 241 MFGILRRNKTVKLEHLILNRKSFAQTVENLFALSFLVKDGRAEITIDKKGSHFVSPKNAP 300
           MFGILRRNKTVKLEHLILNRKSFAQTVENLFALSFLVKDGRAEITIDK GSHFVSPKNAP
Sbjct: 241 MFGILRRNKTVKLEHLILNRKSFAQTVENLFALSFLVKDGRAEITIDKNGSHFVSPKNAP 300

Query: 301 AHSAIMSHEVNYSHFVFRFDYKDWKPMVDMVPVGEELMPHRNFLNSMQVSQEEATADDSE 360
           AHSAIMSHEVNYSHFVFRFDYKDWKPMV+MVPVGEELMPHRNFLNS+ VS+EEATADDSE
Sbjct: 301 AHSAIMSHEVNYSHFVFRFDYKDWKPMVNMVPVGEELMPHRNFLNSVPVSEEEATADDSE 360

Query: 361 SAGLPTTPIRKLSRNRGLVMQSVVEDSPDNVSQSGATAILRGKRKLTQG 410
           SA LPTTPIRKLSRNRGLVMQSVVEDSPDN SQSG TAILRGKRKL+QG
Sbjct: 361 SASLPTTPIRKLSRNRGLVMQSVVEDSPDNGSQSGPTAILRGKRKLSQG 397

[Close]

BLAST of PI0000661 vs. TAIR 10
Match: AT1G51130.1 (Nse4, component of Smc5/6 DNA repair complex )

HSP 1 Score: 353.6 bits (906), Expect = 2.1e-97
Identity = 203/383 (53.00%), Postives = 271/383 (70.76%), Query Frame = 0


Query: 31  SLRDSKRERVARERENSGEENMTEEEVGQQDLTKRRVLRSRYLAVIHEISERREDLSK-D 90
           S++  K+ +   +     E    +EE  +Q ++ RR+LRS+YL++ +EI++ ++DL K D
Sbjct: 27  SVKKEKQRKTEADSVRPDEPPPPQEE--EQGISDRRILRSKYLSLQNEINDCKDDLMKID 86

Query: 91  LDKFNVIINEVEKLHEQVQKPREQVADAEALQDIANSLVTSIRSQSNEGVTPSDFVSCLL 150
            DKF+ IIN VE LH+QV+KPREQ+ADAEAL DIAN+L++S++SQS  GV+P++FV+ L+
Sbjct: 87  SDKFSRIINAVENLHQQVRKPREQIADAEALLDIANTLMSSVKSQSAHGVSPAEFVNALI 146

Query: 151 REFADINGSIKSEERDLVSVNWKDIGLSVSSIFMNGYGCRTMLGPMSNQLKQRKTNNVSR 210
             F   +  I ++E   VS+ WKD+G +V S  +   GC TMLGPM  +LKQRK    +R
Sbjct: 147 SGFGQGSLGIDTDETAQVSLKWKDLGFAVCSTVLVSCGCSTMLGPMDTELKQRK-RAPNR 206

Query: 211 KRSRPTESSRPAEVEDNGSEPKTDTDKNMGIMFGILRRNKTVKLEHLILNRKSFAQTVEN 270
           KR++P E  RP EV+D+ SE KTDTDKNM IMF IL + K V+LE+L+LNR+SFAQTVEN
Sbjct: 207 KRTKPGEGVRPDEVDDSQSEEKTDTDKNMTIMFNILGKKKRVQLENLVLNRRSFAQTVEN 266

Query: 271 LFALSFLVKDGRAEITIDKKGSHFVSPKNAPAHSAIMSHEVNYSHFVFRFDYKDWKPMVD 330
           LFALSFL KDGR EI +DK GSHF  P+NAP  + +MS EV Y+HFVFR D+KDWK M +
Sbjct: 267 LFALSFLAKDGRVEIIVDKSGSHFAMPRNAPDANVVMSGEVIYNHFVFRLDFKDWKLMSE 326

Query: 331 MVPVGEELMPHRNFL---NSMQVSQEEATADDSESAGLPTTPIRKLSRNRGLVMQ--SVV 390
           MVP+GEELMPHR      +S   +   A+AD ++     TTPIRKLSRNRGLV+Q  +VV
Sbjct: 327 MVPLGEELMPHRQTAVASSSCPAASAPASADFTQDT--QTTPIRKLSRNRGLVVQEETVV 386

Query: 391 EDSPDNVSQSGATAILRGKRKLT 408
           ED+PD   + G     R KR+LT
Sbjct: 387 EDTPD---KEGDGTRRRCKRRLT 401

[Close]

BLAST of PI0000661 vs. TAIR 10
Match: AT3G20760.1 (Nse4, component of Smc5/6 DNA repair complex )

HSP 1 Score: 320.1 bits (819), Expect = 2.6e-87
Identity = 173/342 (50.58%), Postives = 242/342 (70.76%), Query Frame = 0


Query: 32  LRDSKRERVARERENSGEENMTEEEVGQQDLTKRRVLRSRYLAVIHEISERREDLSK-DL 91
           ++  KR +         +  + EEE  +Q +  RR +RS+YLA+ H+I + ++DL+K D 
Sbjct: 31  VKKEKRSKTVIASLRPQQPPIQEEE--EQGIADRRAIRSQYLALTHKIKDAKDDLTKIDS 90

Query: 92  DKFNVIINEVEKLHEQVQKPREQVADAEALQDIANSLVTSIRSQS-NEGVTPSDFVSCLL 151
           +KFN IINEVE LH++V+KPREQ+ADAEAL D+ NS+V+S++SQS + GV+P++FV+ L+
Sbjct: 91  NKFNSIINEVENLHQKVRKPREQIADAEALLDLTNSVVSSVKSQSAHGGVSPAEFVNALI 150

Query: 152 REFADINGSIKSEERDLVSVNWKDIGLSVSSIFMNGYGCRTMLGPMSNQLKQRKTNNVSR 211
             F   +  I ++E   VS+ WKD+G +V S  +   GC TM+GPM +++KQRK+   +R
Sbjct: 151 NGFGKTSLRIDADENTQVSMKWKDLGFTVCSTVLVSCGCTTMMGPMYSEMKQRKSRVGNR 210

Query: 212 KRSRPTESSRPAEVEDNGSEPKTDTDKNMGIMFGILRRNKTVKLEHLILNRKSFAQTVEN 271
           KR++P    +P EV+D  +E K+DTD NM +MF ILR+NK VK+E+L+LNRKSFAQT EN
Sbjct: 211 KRTKPGAGVKPEEVDDTEAEKKSDTDNNMAVMFNILRKNKRVKIENLVLNRKSFAQTAEN 270

Query: 272 LFALSFLVKDGRAEITIDKKGSHFVSPKNAPAHSAIMSHEVNYSHFVFRFDYKDWKPMVD 331
           +FALSFLVKDGR EIT+D  GSHFV P+NAPA + ++S EV Y+HFV RFDYKDW+PM  
Sbjct: 271 MFALSFLVKDGRVEITVDNNGSHFVEPRNAPAANLVLSGEVAYNHFVLRFDYKDWEPMSK 330

Query: 332 MVPVGEELMPHRN---FLNSMQVS-------QEEATADDSES 362
           MV VGEELMP R      +S+++S       QEE   +DS +
Sbjct: 331 MVAVGEELMPLRETKVAQDSIELSRKQGSVIQEETVVEDSSN 370

The following BLAST results are available for this feature:

BLAST of PI0000661 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C689	3.0e-96	53.00	Non-structural maintenance of chromosomes element 4 homolog A OS=Arabidopsis tha...	[more]
F4JET1	3.7e-86	50.58	Non-structural maintenance of chromosomes element 4 homolog B OS=Arabidopsis tha...	[more]
Q2TBI1	1.7e-11	22.01	Non-structural maintenance of chromosomes element 4 homolog A OS=Bos taurus OX=9...	[more]
Q6BDR8	2.5e-10	22.97	Non-structural maintenance of chromosome element 4 OS=Schizosaccharomyces pombe ...	[more]
Q9NXX6	5.6e-10	23.03	Non-structural maintenance of chromosomes element 4 homolog A OS=Homo sapiens OX...	[more]

BLAST of PI0000661 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BQS4	5.3e-213	95.84	Non-structural maintenance of chromosomes element 4 OS=Cucumis melo OX=3656 GN=L...	[more]
A0A0A0LZJ0	7.7e-212	95.40	Non-structural maintenance of chromosomes element 4 OS=Cucumis sativus OX=3659 G...	[more]
A0A5A7TLP8	4.5e-204	93.15	Non-structural maintenance of chromosomes element 4 OS=Cucumis melo var. makuwa ...	[more]
A0A6J1HBJ2	4.1e-189	86.83	Non-structural maintenance of chromosomes element 4 OS=Cucurbita moschata OX=366...	[more]
A0A6J1JLE2	5.9e-188	86.31	Non-structural maintenance of chromosomes element 4 OS=Cucurbita maxima OX=3661 ...	[more]

BLAST of PI0000661 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008451119.1	1.1e-212	95.84	PREDICTED: non-structural maintenance of chromosomes element 4 homolog A [Cucumi...	[more]
XP_004149035.1	1.6e-211	95.40	non-structural maintenance of chromosomes element 4 homolog A [Cucumis sativus]	[more]
XP_038879817.1	7.6e-206	92.91	non-structural maintenance of chromosomes element 4 homolog A isoform X1 [Beninc...	[more]
XP_038879818.1	7.6e-206	92.91	non-structural maintenance of chromosomes element 4 homolog A isoform X2 [Beninc...	[more]
KAA0042269.1	9.3e-204	93.15	non-structural maintenance of chromosomes element 4-like protein A [Cucumis melo...	[more]

BLAST of PI0000661 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G51130.1	2.1e-97	53.00	Nse4, component of Smc5/6 DNA repair complex	[more]
AT3G20760.1	2.6e-87	50.58	Nse4, component of Smc5/6 DNA repair complex	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR014854	Non-structural maintenance of chromosome element 4, C-terminal	PFAM	PF08743	Nse4_C	coord: 251..340 e-value: 7.3E-26 score: 90.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..61
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 31..61
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 209..232
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 383..409
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 349..368
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 193..235
None	No IPR available	PANTHER	PTHR16140:SF18	NON-STRUCTURAL MAINTENANCE OF CHROMOSOMES ELEMENT 4	coord: 35..406
IPR027786	Nse4/EID family	PANTHER	PTHR16140	UNCHARACTERIZED	coord: 35..406

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0000661.1	PI0000661.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006310	DNA recombination
biological_process	GO:0006281	DNA repair
cellular_component	GO:0005634	nucleus
cellular_component	GO:0030915	Smc5-Smc6 complex