PI0000555 (gene) Melon (PI 482460) v1

BLAST of PI0000555 vs. ExPASy Swiss-Prot
Match: F4HQM3 (Neutral ceramidase 1 OS=Arabidopsis thaliana OX=3702 GN=NCER1 PE=2 SV=1)

HSP 1 Score: 1178.3 bits (3047), Expect = 0.0e+00
Identity = 577/764 (75.52%), Postives = 653/764 (85.47%), Query Frame = 0

Query: 17  ALFWIFAFLLLQNSESSSSDSNYLIGLGSYDITGPAADVNMMGYANMEQIASGVHFRLRA 76
           + F+I   L L       S S YLIGLGSYDITGPAADVNMMGYANMEQ+ASG+HFRLRA
Sbjct: 15  SFFYISTVLFLLTGGGVYSHSEYLIGLGSYDITGPAADVNMMGYANMEQVASGIHFRLRA 74

Query: 77  RTFIVAEPRGERVVFVNLDACMASQLVTIKLLQRLKARYGDLYTEKNVAISGIHTHAGPG 136
           RTFIV+EP+G+RVVFVNLDACMASQ+V +K+++RLKARYGDLYTE+NV ISGIHTHAGPG
Sbjct: 75  RTFIVSEPQGKRVVFVNLDACMASQIVKLKVIERLKARYGDLYTEQNVGISGIHTHAGPG 134

Query: 137 GYLQYIIYIVTSLGFVPQSFDVLVNGIENSIIQAHENLRPGSIFINKGKLLDAGVNRSPS 196
           GYLQY++YIVTSLGFV QSFD LV+GIENSIIQAHENLRPGSIF+N G+LLDAGVNRSPS
Sbjct: 135 GYLQYVVYIVTSLGFVRQSFDALVDGIENSIIQAHENLRPGSIFLNNGELLDAGVNRSPS 194

Query: 197 AYLNNPAEERGKYKYNVDKEMTLLKFVDLEWGPIGSFNWFATHGTSMSRTNSLISGDNKG 256
           AYLNNP++ER K+KYNVDKEMTLLKFVD +WGP+GSFNWFATHGTSMSRTNSLISGDNKG
Sbjct: 195 AYLNNPSKERSKHKYNVDKEMTLLKFVDDQWGPVGSFNWFATHGTSMSRTNSLISGDNKG 254

Query: 257 AAARFMEDWFEKKSGISSVFDISVDDRIPRRVSSIIPDLFINHHELLELAASFQSPSGGS 316
           AA+RFMEDW+E+ +   S  +  + D IPRRVSS+I +   +HHELLELA+ F+S  G  
Sbjct: 255 AASRFMEDWYEQNTAERSYSEEFISDEIPRRVSSLIENHQDSHHELLELASYFESQPGKP 314

Query: 317 ATRILSSSRRVRGALRNAEKPQFVSAFCQSNCGDVSPNVLGAFCIDTGLSCDFNHSTCNG 376
            TRI SS+RRVR ALR A+KP FVSAFCQ+NCGDVSPNVLGAFC+DTGL CDFNHSTC G
Sbjct: 315 VTRISSSARRVRSALRKADKPGFVSAFCQTNCGDVSPNVLGAFCLDTGLPCDFNHSTCGG 374

Query: 377 KNELCYGRGPGYPDEFESTRIIGKRQFRKAVDLFSNASEQLQGKVDYRHSYVDFSKLEVA 436
           KNE+CYGRGPGYPDEFESTRIIG+RQF+ A++LF+ ASEQLQGKVDYRH YVDFS+L V 
Sbjct: 375 KNEMCYGRGPGYPDEFESTRIIGERQFKMALELFNKASEQLQGKVDYRHVYVDFSQLNVT 434

Query: 437 I-ECGGTTEVVQTCPAAMGYSFAAGTTDGPGAFDFEQGDDKGNPFWKHIRNFLKKPKKEQ 496
           + +  G +EVV+TCPAAMG++FAAGTTDGPGAFDF QGDDKGNPFW+ +RN LK P K+Q
Sbjct: 435 LPKKDGKSEVVKTCPAAMGFAFAAGTTDGPGAFDFTQGDDKGNPFWRLVRNVLKTPDKKQ 494

Query: 497 IDCQQPKPILLDTGEMTKPYYWAPSILPIQILRIGQLIILGVPGEFSTMAGRRLRDAVQT 556
           IDC  PKPILLDTGEMTKPY WAPSIL +Q+LRIGQL IL VPGEF+TMAGRRLR AV+T
Sbjct: 495 IDCHYPKPILLDTGEMTKPYDWAPSILSLQVLRIGQLFILSVPGEFTTMAGRRLRYAVKT 554

Query: 557 VLTDGDKSEFSGDIHIVIAGLTNSYSQYVTTFEEYQVQRYEGASTLYGPHTLSAYIQEFK 616
            L +    + SG+IH+VIAGL N YSQYVTTFEEYQVQRYEGASTLYGPHTLS YIQEFK
Sbjct: 555 QLKNSGNKDLSGEIHVVIAGLANGYSQYVTTFEEYQVQRYEGASTLYGPHTLSGYIQEFK 614

Query: 617 KLARALVDGQAVGAGPQPPDLLDKQISFLPSVVMDTTPHSVKFGDVKSDVPKNSTFKRGD 676
           KL+++LV    V  GPQPPDLLDKQ+SFL  V+MDTTP    FGDV SDVPKN + KRG+
Sbjct: 615 KLSKSLVLDMPVQPGPQPPDLLDKQLSFLTPVMMDTTPSGDSFGDVISDVPKNLSLKRGN 674

Query: 677 -TVTVTFWSACPRNDLMTEGTYALVEILHDK-KLWVPAYDDDDFCLRFKWSRPFKLSSQS 736
             VTV F SACPRNDL+TEGT+ LVE L  K K W P YDDDD CLRFKWSR  KLSS+S
Sbjct: 675 GQVTVVFRSACPRNDLLTEGTFTLVERLEQKDKTWTPVYDDDDLCLRFKWSRHKKLSSRS 734

Query: 737 HATIEWRIPKSATPGVYRITHFGAAKKLFGSISHFTGSSSAFGV 778
            AT+EWRIP+SA+PGVYRITHFGAAKKLFGS+ HFTGSSSAF V
Sbjct: 735 QATVEWRIPESASPGVYRITHFGAAKKLFGSVHHFTGSSSAFVV 778

BLAST of PI0000555 vs. ExPASy Swiss-Prot
Match: Q304B9 (Neutral ceramidase 2 OS=Arabidopsis thaliana OX=3702 GN=NCER2 PE=3 SV=1)

HSP 1 Score: 1121.7 bits (2900), Expect = 0.0e+00
Identity = 551/764 (72.12%), Postives = 637/764 (83.38%), Query Frame = 0

Query: 18  LFWIFAFLLLQNSESSSSDSNYLIGLGSYDITGPAADVNMMGYANMEQIASGVHFRLRAR 77
           LF    FLLL     S +   YLIG+GSYDITGPAADVNMMGYAN +QIASG+HFRLRAR
Sbjct: 7   LFQFILFLLL--LLLSRTVYAYLIGVGSYDITGPAADVNMMGYANSDQIASGIHFRLRAR 66

Query: 78  TFIVAEPRGERVVFVNLDACMASQLVTIKLLQRLKARYGDLYTEKNVAISGIHTHAGPGG 137
            FIVAEP+G RVVFVNLDACMASQ+VTIK+L+RLKARYG+LYTEKNVAISGIHTHAGPGG
Sbjct: 67  AFIVAEPQGNRVVFVNLDACMASQIVTIKVLERLKARYGELYTEKNVAISGIHTHAGPGG 126

Query: 138 YLQYIIYIVTSLGFVPQSFDVLVNGIENSIIQAHENLRPGSIFINKGKLLDAGVNRSPSA 197
           YLQY+ YIVTSLGFV QSFDV+VNGIE SI+QAHE+LRPGS F+NKG LLDAGVNRSPS+
Sbjct: 127 YLQYVTYIVTSLGFVRQSFDVVVNGIEQSIVQAHESLRPGSAFVNKGDLLDAGVNRSPSS 186

Query: 198 YLNNPAEERGKYKYNVDKEMTLLKFVDLEWGPIGSFNWFATHGTSMSRTNSLISGDNKGA 257
           YLNNPA ER KYKY+VDKEMTL+KFVD + GP GSFNWFATHGTSMSRTNSLISGDNKGA
Sbjct: 187 YLNNPAAERSKYKYDVDKEMTLVKFVDSQLGPTGSFNWFATHGTSMSRTNSLISGDNKGA 246

Query: 258 AARFMEDWFE--KKSGISSVFDISVDDRIPRRVSSIIPDLFINHHELLELAASFQSPSGG 317
           AARFMEDWFE  +K+ +SS         IPRRVS+I+ D   N   LL++AA+++S  G 
Sbjct: 247 AARFMEDWFENGQKNSVSS-------RNIPRRVSTIVSDFSRNRDRLLDIAATYKSSRGH 306

Query: 318 SATRILSSSRRVRGALRNAEKPQFVSAFCQSNCGDVSPNVLGAFCIDTGLSCDFNHSTCN 377
           S  + L    RV    RN  K +FVSAFCQSNCGDVSPN LG FCIDTGL CDFNHSTCN
Sbjct: 307 SVDKSLDVKTRV----RNGSKRKFVSAFCQSNCGDVSPNTLGTFCIDTGLPCDFNHSTCN 366

Query: 378 GKNELCYGRGPGYPDEFESTRIIGKRQFRKAVDLFSNASEQLQGKVDYRHSYVDFSKLEV 437
           G+NELCYGRGPGYPDEFESTRIIG++QF+ AV+LF+ A+E+LQGK+ Y+H+Y+DFS L+V
Sbjct: 367 GQNELCYGRGPGYPDEFESTRIIGEKQFKMAVELFNKATEKLQGKIGYQHAYLDFSNLDV 426

Query: 438 AI-ECGGTTEVVQTCPAAMGYSFAAGTTDGPGAFDFEQGDDKGNPFWKHIRNFLKKPKKE 497
            + + GG +E V+TCPAAMG+ FAAGTTDGPGAFDF+QGDD+GN FW+ +RN L+ P  E
Sbjct: 427 TVPKAGGGSETVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNVFWRLVRNVLRTPGPE 486

Query: 498 QIDCQQPKPILLDTGEMTKPYYWAPSILPIQILRIGQLIILGVPGEFSTMAGRRLRDAVQ 557
           Q+ CQ+PKPILLDTGEM +PY WAPSILPIQILRIGQL+IL VPGEF+TMAGRRLRDA++
Sbjct: 487 QVQCQKPKPILLDTGEMKEPYDWAPSILPIQILRIGQLVILSVPGEFTTMAGRRLRDAIK 546

Query: 558 TVLTDGDKSEFSGDIHIVIAGLTNSYSQYVTTFEEYQVQRYEGASTLYGPHTLSAYIQEF 617
           + L   D  EFS ++H+VIAGLTN+YSQY+ TFEEY+VQRYEGASTLYG HTL+AYIQEF
Sbjct: 547 SFLISSDPKEFSNNMHVVIAGLTNTYSQYIATFEEYEVQRYEGASTLYGRHTLTAYIQEF 606

Query: 618 KKLARALVDGQAVGAGPQPPDLLDKQISFLPSVVMDTTPHSVKFGDVKSDVPKNSTFKRG 677
           KKLA ALV+G  +  GPQPPDLLDKQIS L  VV+D+TP  VKFGDVK+DVP  STF+RG
Sbjct: 607 KKLATALVNGLTLPRGPQPPDLLDKQISLLSPVVVDSTPLGVKFGDVKADVPPKSTFRRG 666

Query: 678 DTVTVTFWSACPRNDLMTEGTYALVEILHDKKLWVPAYDDDDFCLRFKWSRPFKLSSQSH 737
             V  TFWS CPRNDLMTEG++A+VE L +   W P YDDDDF L+FKWSRP KLSS+S 
Sbjct: 667 QQVNATFWSGCPRNDLMTEGSFAVVETLREGGKWAPVYDDDDFSLKFKWSRPAKLSSESQ 726

Query: 738 ATIEWRIPKSATPGVYRITHFGAAKKLFGSISHFTGSSSAFGVM 779
           ATIEWR+P+SA  GVYRI H+GA+K LFGSIS F+GSSSAF V+
Sbjct: 727 ATIEWRVPESAVAGVYRIRHYGASKSLFGSISSFSGSSSAFVVV 757

BLAST of PI0000555 vs. ExPASy Swiss-Prot
Match: Q0JL46 (Neutral ceramidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0624000 PE=1 SV=1)

HSP 1 Score: 1115.9 bits (2885), Expect = 0.0e+00
Identity = 543/767 (70.80%), Postives = 634/767 (82.66%), Query Frame = 0

Query: 20  WIFAFLLLQNSESSSSDSNYLIGLGSYDITGPAADVNMMGYANMEQIASGVHFRLRARTF 79
           W+   L+L N     S S YL+G+GS+DITGPAADVNMMGYAN EQIASG+HFRL++R F
Sbjct: 22  WLLLALVLLNCSLVLSASPYLVGMGSFDITGPAADVNMMGYANTEQIASGIHFRLKSRAF 81

Query: 80  IVAEPRGERVVFVNLDACMASQLVTIKLLQRLKARYGDLYTEKNVAISGIHTHAGPGGYL 139
           IVAEP G+RVVFVN+DACMASQ+VTIK+L+RLKARYGDLY E NVAISGIHTHAGPGGYL
Sbjct: 82  IVAEPNGKRVVFVNIDACMASQIVTIKVLERLKARYGDLYNENNVAISGIHTHAGPGGYL 141

Query: 140 QYIIYIVTSLGFVPQSFDVLVNGIENSIIQAHENLRPGSIFINKGKLLDAGVNRSPSAYL 199
           QY++YIVTSLGFV QSFDV+V+GIE SI++AH NLRPG IF+NKG LLDAGVNRSPSAYL
Sbjct: 142 QYVVYIVTSLGFVRQSFDVIVDGIEQSIVEAHNNLRPGKIFVNKGDLLDAGVNRSPSAYL 201

Query: 200 NNPAEERGKYKYNVDKEMTLLKFVDLEWGPIGSFNWFATHGTSMSRTNSLISGDNKGAAA 259
           NNPAEER KY+YNVDKEMTL+KFVD E GP+GSFNWFATHGTSMSRTNSLISGDNKGAAA
Sbjct: 202 NNPAEERSKYEYNVDKEMTLIKFVDDELGPVGSFNWFATHGTSMSRTNSLISGDNKGAAA 261

Query: 260 RFMEDWFEKKSGISSVFDISVDDR--------IPRRVSSIIPDLFINHHELLELAASFQS 319
           RFMEDW E+          + DD         +PRRVS+IIP+      +L++LA+S+++
Sbjct: 262 RFMEDWAEQMGLPKQSAHANSDDLRSLHKTSVLPRRVSTIIPEPNEITDDLIQLASSYEA 321

Query: 320 PSGGSATRILSSSRRVRGALRNAEKPQFVSAFCQSNCGDVSPNVLGAFCIDTGLSCDFNH 379
            SGG      S +RR+R   +N  KP+FVSAFCQSNCGDVSPNVLG FCIDT L CDFNH
Sbjct: 322 -SGGRRLAGSSITRRIRSTQQN--KPKFVSAFCQSNCGDVSPNVLGTFCIDTNLPCDFNH 381

Query: 380 STCNGKNELCYGRGPGYPDEFESTRIIGKRQFRKAVDLFSNASEQLQGKVDYRHSYVDFS 439
           STCNGKNELCYGRGPGYPDEFESTR+IG RQF KA DLF +ASE++QGK+DYRH+Y+DFS
Sbjct: 382 STCNGKNELCYGRGPGYPDEFESTRVIGNRQFLKARDLFDSASEEIQGKIDYRHTYLDFS 441

Query: 440 KLEVAIE-CGGTTEVVQTCPAAMGYSFAAGTTDGPGAFDFEQGDDKGNPFWKHIRNFLKK 499
           KLEV +    G  + V+TCPAAMG++FAAGTTDGPGAFDF QGD KGNPFWK +RN LK 
Sbjct: 442 KLEVKVSTSAGGQQTVKTCPAAMGFAFAAGTTDGPGAFDFRQGDVKGNPFWKLVRNLLKT 501

Query: 500 PKKEQIDCQQPKPILLDTGEMTKPYYWAPSILPIQILRIGQLIILGVPGEFSTMAGRRLR 559
           P K+Q++C  PKPILLDTGEM +PY WAP+ILP+Q++RIGQL+IL VPGEF+TMAGRRLR
Sbjct: 502 PGKDQVECHSPKPILLDTGEMKEPYDWAPAILPVQMIRIGQLVILCVPGEFTTMAGRRLR 561

Query: 560 DAVQTVLTDGDKSEFSGDIHIVIAGLTNSYSQYVTTFEEYQVQRYEGASTLYGPHTLSAY 619
           DAV+TVLT G+ SEF  +IH+V+AGLTNSYSQY+TTFEEYQ+QRYEGASTLYGPHTLSAY
Sbjct: 562 DAVKTVLTSGN-SEFDKNIHVVLAGLTNSYSQYITTFEEYQIQRYEGASTLYGPHTLSAY 621

Query: 620 IQEFKKLARALVDGQAVGAGPQPPDLLDKQISFLPSVVMDTTPHSVKFGDVKSDVPKNST 679
           IQEF+KLA A++  + V    QPPD+LDKQI  LP VV D+TP  VKFGDV SDVP NST
Sbjct: 622 IQEFQKLAMAMIANKEVPTNFQPPDMLDKQIGLLPGVVFDSTPLGVKFGDVNSDVPGNST 681

Query: 680 FKRGDTVTVTFWSACPRNDLMTEGTYALVEILHDKKLWVPAYDDDDFCLRFKWSRPFKLS 739
           F +G TV  TF+SACPRNDL+T+GT+ALVE L     WVP YDDDD+ LRFKWSRP +LS
Sbjct: 682 FNKGSTVNATFYSACPRNDLLTDGTFALVEKLDGNNNWVPVYDDDDWSLRFKWSRPARLS 741

Query: 740 SQSHATIEWRIPKSATPGVYRITHFGAAKKLFGSISHFTGSSSAFGV 778
           S+S AT+EW +P+ A  GVYR+ HFGA+K +FGS+ HFTG+S AF V
Sbjct: 742 SRSFATLEWTVPEDAAAGVYRLRHFGASKPMFGSVRHFTGTSRAFAV 784

BLAST of PI0000555 vs. ExPASy Swiss-Prot
Match: F4KHQ8 (Neutral ceramidase 3 OS=Arabidopsis thaliana OX=3702 GN=NCER3 PE=2 SV=1)

HSP 1 Score: 1047.7 bits (2708), Expect = 6.3e-305
Identity = 531/747 (71.08%), Postives = 605/747 (80.99%), Query Frame = 0

Query: 35  SDSNYLIGLGSYDITGPAADVNMMGYANMEQIASGVHFRLRARTFIVAEPRGERVVFVNL 94
           SDS+YL+GLGSYDITGPAADVNMMGYANMEQ+ASGVHFRLRAR FIVAEP  +R+ FVNL
Sbjct: 25  SDSDYLMGLGSYDITGPAADVNMMGYANMEQVASGVHFRLRARAFIVAEPYKKRIAFVNL 84

Query: 95  DACMASQLVTIKLLQRLKARYGDLYTEKNVAISGIHTHAGPGGYLQYIIYIVTSLGFVPQ 154
           DA MASQLVTIK+++RLK RYG+LYTE+NVAISG HTHAGPGGYLQYI+Y+VTSLGFV Q
Sbjct: 85  DAGMASQLVTIKVIERLKQRYGELYTEENVAISGTHTHAGPGGYLQYILYLVTSLGFVHQ 144

Query: 155 SFDVLVNGIENSIIQAHENLRPGSIFINKGKLLDAGVNRSPSAYLNNPAEERGKYKYNVD 214
           SF+ LV+GIE SIIQAHENLRPGSI INKG+LLDAGVNRSPSAYLNNPA ER KY+Y+VD
Sbjct: 145 SFNALVDGIEQSIIQAHENLRPGSILINKGELLDAGVNRSPSAYLNNPAHERSKYEYDVD 204

Query: 215 KEMTLLKFVDLEWGPIGSFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWFEKKSGISS 274
           KEMTL+KFVD +WGP+                            AR MEDWFE+++G   
Sbjct: 205 KEMTLVKFVDDQWGPV----------------------------ARIMEDWFERENGCR- 264

Query: 275 VFDISVDDRIPRRVSSIIPDLFINHHELLELAASFQSPSGGSATRILSSSRRVRGALRNA 334
               SVD   PRRVSSII D +    +L+E+A+S  S  G + TR+ S +RRVR   R+A
Sbjct: 265 ----SVDVESPRRVSSIISDPY--DQDLMEMASSLLSTGGKTVTRMSSVARRVRSRFRHA 324

Query: 335 EKPQFVSAFCQSNCGDVSPNVLGAFCIDTGLSCDFNHSTCNGKNELCYGRGPGYPDEFES 394
           +KP+FVSAFCQ+NCGDVSPNVLGAFCIDTGL C+FN STC GKNE CYGRGPGYPDEFES
Sbjct: 325 DKPRFVSAFCQTNCGDVSPNVLGAFCIDTGLPCEFNQSTCGGKNEQCYGRGPGYPDEFES 384

Query: 395 TRIIGKRQFRKAVDLFSNASEQLQGKVDYRHSYVDFSKLEVAIE-CGGTTEVVQTCPAAM 454
           TRIIG+RQF+KA DLF+ ASE++QGKVDYRH+YVDFS+LEV I    G +EVV+TCPAAM
Sbjct: 385 TRIIGERQFKKAADLFTKASEEIQGKVDYRHAYVDFSQLEVTINGQNGGSEVVKTCPAAM 444

Query: 455 GYSFAAGTTDGPGAFDFEQGDDKGNPFWKHIRNFLKKPKKEQIDCQQPKPILLDTGEMTK 514
           G+ FAAGTTDGPGAFDF+QGDD+GNPFW+ +RN LK P +EQ+ CQ+PKPILLDTGEM +
Sbjct: 445 GFGFAAGTTDGPGAFDFKQGDDQGNPFWRLVRNLLKNPTEEQVRCQRPKPILLDTGEMKQ 504

Query: 515 PYYWAPSILPIQILRIGQLIILGVPGEFSTMAGRRLRDAVQTVLTDGDKS-EFSGDIHIV 574
           PY WAPSILP+QILRIGQL+IL VPGEF+TMAGRRLRDAV+TVL +G    EFS    +V
Sbjct: 505 PYDWAPSILPVQILRIGQLVILCVPGEFTTMAGRRLRDAVKTVLKEGSNGREFS----VV 564

Query: 575 IAGLTNSYSQYVTTFEEYQVQRYEGASTLYGPHTLSAYIQEFKKLARALVDGQAVGAGPQ 634
           IAGLTNSYSQY+ TFEEYQVQRYEGASTLYGPHTLS YIQEFKKLA  L+  Q    GPQ
Sbjct: 565 IAGLTNSYSQYIATFEEYQVQRYEGASTLYGPHTLSGYIQEFKKLANDLLSAQTTDPGPQ 624

Query: 635 PPDLLDKQISFLPSVVMDTTPHSVKFGDVKSDVPKNSTFKRG-DTVTVTFWSACPRNDLM 694
           PPDLL KQIS L  VV D TP    FGDV SDVP+ S F++G D V V F SA PRNDLM
Sbjct: 625 PPDLLHKQISLLTPVVADMTPIGTAFGDVTSDVPRLSKFRKGADIVRVQFRSANPRNDLM 684

Query: 695 TEGTYALVE-ILHDKKLWVPAYDDDDFCLRFKWSRPFKLSSQSHATIEWRIPKSATPGVY 754
           TEGT+ALVE  L  ++ WVP YDDDDFCLRFKWSRPFKLS+QS ATIEWRIP++A+PGVY
Sbjct: 685 TEGTFALVERWLEGRETWVPVYDDDDFCLRFKWSRPFKLSTQSTATIEWRIPETASPGVY 732

Query: 755 RITHFGAAKKLFGSISHFTGSSSAFGV 778
           RITHFG+AK    SI HF+GSSSAF V
Sbjct: 745 RITHFGSAKTPISSIHHFSGSSSAFVV 732

BLAST of PI0000555 vs. ExPASy Swiss-Prot
Match: Q55G11 (Neutral ceramidase B OS=Dictyostelium discoideum OX=44689 GN=dcd2B PE=3 SV=1)

HSP 1 Score: 628.2 bits (1619), Expect = 1.2e-178
Identity = 339/756 (44.84%), Postives = 457/756 (60.45%), Query Frame = 0

Query: 28  QNSESSSSDSNYLIGLGSYDITGPAADVNMMGYANMEQIASGVHFRLRARTFIVAEPRGE 87
           Q +  SS DS+Y IG G YDITG +A+VN+MGYAN  Q+ +G+HFR RAR F+  +  G 
Sbjct: 35  QKNVKSSGDSSYQIGAGIYDITGASAEVNLMGYANPLQVGAGIHFRQRARAFVFVDSNGN 94

Query: 88  RVVFVNLDACMASQLVTIKLLQRLKARYG-DLYTEKNVAISGIHTHAGPGGYLQYIIYIV 147
           R V+V+ D+CM  Q V I +++ L+  +G ++YTE NV +SG HTH+GP G+ QY +Y +
Sbjct: 95  RAVYVSTDSCMIFQEVKIHVVELLQDIFGPNVYTEANVLLSGTHTHSGPAGFSQYALYGI 154

Query: 148 TSLGFVPQSFDVLVNGIENSIIQAHENLRPGSIFINKGKLLDAGVNRSPSAYLNNPAEER 207
           TSLGF  ++FD + NGI  +I++AH++++P ++F   G+L +  +NRSP AY NNP EE+
Sbjct: 155 TSLGFYKKNFDTICNGIVQAIVKAHKSVQPANMFTETGELWNTNINRSPFAYDNNPEEEK 214

Query: 208 GKYKYNVDKEMTLLKFVDLEWGPIGSFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWF 267
             Y  NVDK MT+L+  D+   P  + ++FA H TSM+ TN LISGDNKG A+   E   
Sbjct: 215 AMYDSNVDKNMTVLRIEDMNGNPFAAISFFAVHCTSMNNTNHLISGDNKGYASYLWE--- 274

Query: 268 EKKSGISSVFDISVDDRIPRRVSSIIPDLFINHHELLELAASFQSPSGGSATRILSSSRR 327
                                                                     ++
Sbjct: 275 ----------------------------------------------------------KQ 334

Query: 328 VRGALRNAEKPQFVSAFCQSNCGDVSPNVLGAFCIDTGLSCDFNHSTCNGKNELCYGRGP 387
           V G    A K  FV+AF QSN GDVSPN  G  C D G  CD+  STCNG+NE C+  GP
Sbjct: 335 VNGP-GTAGKGPFVAAFGQSNEGDVSPNTRGPTCRD-GSPCDYKTSTCNGRNEECWSLGP 394

Query: 388 GYP-DEFESTRIIGKRQFRKAVDLFSNASEQLQGKVDYRHSYVDFSKLEVAIECGGTTEV 447
           G   D FEST+IIG  QF KA++LF+NAS Q+ G V YRHS+V F+ + V        + 
Sbjct: 395 GKDGDMFESTQIIGGNQFNKALELFNNASIQVSGPVQYRHSWVQFTNVSVEPPYNSGVDN 454

Query: 448 VQTCPAAMGYSFAAGTTDGPGAFDFEQGDD--KGNPFWKHIRNFLKKPKKEQIDCQQPKP 507
             TC  AMGYSFAAGTTDGPGAF+F Q D+   GNPFW  I +F+ KP  +QI CQ PKP
Sbjct: 455 ATTCRGAMGYSFAAGTTDGPGAFNFVQSDNNTSGNPFWNFIGDFIAKPTPDQIRCQSPKP 514

Query: 508 ILLDTGEMTKPYYWAPSILPIQILRIGQLIILGVPGEFSTMAGRRLRDAVQTVLTDGDKS 567
           ILLD G M +P  W P ++PIQI+ IGQ++++ VPGEF+TM+GRRLR++V+ ++ +  ++
Sbjct: 515 ILLDVG-MVEPIPWVPDVMPIQIVTIGQIVLVAVPGEFTTMSGRRLRNSVREIIGESIEN 574

Query: 568 EFSGDIHIVIAGLTNSYSQYVTTFEEYQVQRYEGASTLYGPHTLSAYIQEFKKLARALVD 627
                  ++IAGL+N+YS Y+ TFEEYQVQRYEGAST++GPHTL +Y+QEF KLA+++VD
Sbjct: 575 PI-----VLIAGLSNTYSGYIATFEEYQVQRYEGASTVFGPHTLGSYMQEFGKLAQSIVD 634

Query: 628 GQAVGAGPQPPDLLDKQISFLPSVVMDTTPHSVKFGDVKSDVPKNSTFKRGDTVTVTFWS 687
           G  V AGP P +L    + FLP V++D  P    FG+V  DV  N  +   +TV+  F+ 
Sbjct: 635 GTTVPAGPTPRNLTGHTLFFLPPVIVDAAPDFDDFGEVSIDV--NLNYSVNETVSCVFYG 694

Query: 688 ACPRNDLMTEGTYALVEILHDKKLWVPAYDDDDFCLRFKWSRPFKLSSQSHATIEWRIPK 747
             PRND M E ++  V++L     W    DD D+  +FKW         S  TIEW I  
Sbjct: 695 GNPRNDFMIESSFLSVDLLTGTDQWTTVLDDGDWDTKFKWK--MHDLGFSLITIEWVIAP 717

Query: 748 SATPGVYRITHFGAAKK--LFGSISHFTGSSSAFGV 778
             TPG YRITH G AKK     +++ + G S  F V
Sbjct: 755 DTTPGTYRITHSGFAKKNPFSSNLTPYQGISRNFVV 717

BLAST of PI0000555 vs. ExPASy TrEMBL
Match: A0A5D3CV52 (Neutral ceramidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold571G00370 PE=3 SV=1)

HSP 1 Score: 1537.7 bits (3980), Expect = 0.0e+00
Identity = 760/779 (97.56%), Postives = 768/779 (98.59%), Query Frame = 0

Query: 1   MEFVSLFNFSIRRPCEALFWIFAFLLLQNSE-SSSSDSNYLIGLGSYDITGPAADVNMMG 60
           MEFVSLFNFSIRRPCEALFWIFAFLLLQNSE SSSSDSNYLIGLGSYDITGPAADVNMMG
Sbjct: 1   MEFVSLFNFSIRRPCEALFWIFAFLLLQNSESSSSSDSNYLIGLGSYDITGPAADVNMMG 60

Query: 61  YANMEQIASGVHFRLRARTFIVAEPRGERVVFVNLDACMASQLVTIKLLQRLKARYGDLY 120
           YANMEQIASGVHFRLRARTFIVAEP+GERVVFVNLDACMASQLVTIKLLQRLK RYGDLY
Sbjct: 61  YANMEQIASGVHFRLRARTFIVAEPQGERVVFVNLDACMASQLVTIKLLQRLKERYGDLY 120

Query: 121 TEKNVAISGIHTHAGPGGYLQYIIYIVTSLGFVPQSFDVLVNGIENSIIQAHENLRPGSI 180
           TEKNVAISGIHTHAGPGGYLQYIIYIVTSLGFV QSFDVLVNGIENSIIQAHENLRPGSI
Sbjct: 121 TEKNVAISGIHTHAGPGGYLQYIIYIVTSLGFVRQSFDVLVNGIENSIIQAHENLRPGSI 180

Query: 181 FINKGKLLDAGVNRSPSAYLNNPAEERGKYKYNVDKEMTLLKFVDLEWGPIGSFNWFATH 240
           FINKGKLLDAGVNRSPSAYLNNPAEERGKYKYNVDKEMTLLKFVDLEWGPIGSF+WFATH
Sbjct: 181 FINKGKLLDAGVNRSPSAYLNNPAEERGKYKYNVDKEMTLLKFVDLEWGPIGSFSWFATH 240

Query: 241 GTSMSRTNSLISGDNKGAAARFMEDWFEKKSGISSVFDISVDDRIPRRVSSIIPDLFINH 300
           GTSMSRTNSLISGDNKGAAARFMEDWFEKKSGISSVF+ISVDDRIPRRVSSIIPDLFINH
Sbjct: 241 GTSMSRTNSLISGDNKGAAARFMEDWFEKKSGISSVFNISVDDRIPRRVSSIIPDLFINH 300

Query: 301 HELLELAASFQSPSGGSATRILSSSRRVRGALRNAEKPQFVSAFCQSNCGDVSPNVLGAF 360
           HELLELAASFQSPSG SATRILSSSRRVRGALRNAEKPQFVSAFCQSNCGDVSPNVLGAF
Sbjct: 301 HELLELAASFQSPSGRSATRILSSSRRVRGALRNAEKPQFVSAFCQSNCGDVSPNVLGAF 360

Query: 361 CIDTGLSCDFNHSTCNGKNELCYGRGPGYPDEFESTRIIGKRQFRKAVDLFSNASEQLQG 420
           CIDTGL CDFNHSTCNGKNELCYGRGPGYPDEFESTRIIGKRQFRKAV LFSNASEQLQG
Sbjct: 361 CIDTGLPCDFNHSTCNGKNELCYGRGPGYPDEFESTRIIGKRQFRKAVALFSNASEQLQG 420

Query: 421 KVDYRHSYVDFSKLEVAIECGGTTEVVQTCPAAMGYSFAAGTTDGPGAFDFEQGDDKGNP 480
           KVDYRHSYVDFSKLEV IE GGTTEVVQTCPAAMGYSFAAGTTDGPGAFDFEQGDDKGNP
Sbjct: 421 KVDYRHSYVDFSKLEVTIERGGTTEVVQTCPAAMGYSFAAGTTDGPGAFDFEQGDDKGNP 480

Query: 481 FWKHIRNFLKKPKKEQIDCQQPKPILLDTGEMTKPYYWAPSILPIQILRIGQLIILGVPG 540
           FWKHIRNFLKKPKKEQIDCQ PKPILLDTGEMTKPYYWAPSILPIQILR+GQLIILGVPG
Sbjct: 481 FWKHIRNFLKKPKKEQIDCQHPKPILLDTGEMTKPYYWAPSILPIQILRVGQLIILGVPG 540

Query: 541 EFSTMAGRRLRDAVQTVLTDGDKSEFSGDIHIVIAGLTNSYSQYVTTFEEYQVQRYEGAS 600
           EFSTMAGRRLRDAV+TVLTDGDKSEFS DIHIVIAGLTNSYSQYVTTFEEYQVQRYEGAS
Sbjct: 541 EFSTMAGRRLRDAVKTVLTDGDKSEFSDDIHIVIAGLTNSYSQYVTTFEEYQVQRYEGAS 600

Query: 601 TLYGPHTLSAYIQEFKKLARALVDGQAVGAGPQPPDLLDKQISFLPSVVMDTTPHSVKFG 660
           TLYGPHTLSAYIQEFKKLAR+LVDGQAV AGPQPPDLLDKQISFLPSVVMDTTPHSVKFG
Sbjct: 601 TLYGPHTLSAYIQEFKKLARSLVDGQAVAAGPQPPDLLDKQISFLPSVVMDTTPHSVKFG 660

Query: 661 DVKSDVPKNSTFKRGDTVTVTFWSACPRNDLMTEGTYALVEILHDKKLWVPAYDDDDFCL 720
           DV+SDVPKNSTFKRGDTVTVTFWSACPRNDLMTEGTYALVEILHDKKLWVPAYDDDDFCL
Sbjct: 661 DVESDVPKNSTFKRGDTVTVTFWSACPRNDLMTEGTYALVEILHDKKLWVPAYDDDDFCL 720

Query: 721 RFKWSRPFKLSSQSHATIEWRIPKSATPGVYRITHFGAAKKLFGSISHFTGSSSAFGVM 779
           RFKWSRPFKLSSQSHATIEWRIPKSATPGVYRITHFGAAK+LFGSISHFTGSSSAFGVM
Sbjct: 721 RFKWSRPFKLSSQSHATIEWRIPKSATPGVYRITHFGAAKRLFGSISHFTGSSSAFGVM 779

BLAST of PI0000555 vs. ExPASy TrEMBL
Match: A0A1S3CM50 (Neutral ceramidase OS=Cucumis melo OX=3656 GN=LOC103502048 PE=3 SV=1)

HSP 1 Score: 1537.7 bits (3980), Expect = 0.0e+00
Identity = 760/779 (97.56%), Postives = 768/779 (98.59%), Query Frame = 0

Query: 1   MEFVSLFNFSIRRPCEALFWIFAFLLLQNSE-SSSSDSNYLIGLGSYDITGPAADVNMMG 60
           MEFVSLFNFSIRRPCEALFWIFAFLLLQNSE SSSSDSNYLIGLGSYDITGPAADVNMMG
Sbjct: 1   MEFVSLFNFSIRRPCEALFWIFAFLLLQNSESSSSSDSNYLIGLGSYDITGPAADVNMMG 60

Query: 61  YANMEQIASGVHFRLRARTFIVAEPRGERVVFVNLDACMASQLVTIKLLQRLKARYGDLY 120
           YANMEQIASGVHFRLRARTFIVAEP+GERVVFVNLDACMASQLVTIKLLQRLK RYGDLY
Sbjct: 61  YANMEQIASGVHFRLRARTFIVAEPQGERVVFVNLDACMASQLVTIKLLQRLKERYGDLY 120

Query: 121 TEKNVAISGIHTHAGPGGYLQYIIYIVTSLGFVPQSFDVLVNGIENSIIQAHENLRPGSI 180
           TEKNVAISGIHTHAGPGGYLQYIIYIVTSLGFV QSFDVLVNGIENSIIQAHENLRPGSI
Sbjct: 121 TEKNVAISGIHTHAGPGGYLQYIIYIVTSLGFVRQSFDVLVNGIENSIIQAHENLRPGSI 180

Query: 181 FINKGKLLDAGVNRSPSAYLNNPAEERGKYKYNVDKEMTLLKFVDLEWGPIGSFNWFATH 240
           FINKGKLLDAGVNRSPSAYLNNPAEERGKYKYNVDKEMTLLKFVDLEWGPIGSF+WFATH
Sbjct: 181 FINKGKLLDAGVNRSPSAYLNNPAEERGKYKYNVDKEMTLLKFVDLEWGPIGSFSWFATH 240

Query: 241 GTSMSRTNSLISGDNKGAAARFMEDWFEKKSGISSVFDISVDDRIPRRVSSIIPDLFINH 300
           GTSMSRTNSLISGDNKGAAARFMEDWFEKKSGISSVF+ISVDDRIPRRVSSIIPDLFINH
Sbjct: 241 GTSMSRTNSLISGDNKGAAARFMEDWFEKKSGISSVFNISVDDRIPRRVSSIIPDLFINH 300

Query: 301 HELLELAASFQSPSGGSATRILSSSRRVRGALRNAEKPQFVSAFCQSNCGDVSPNVLGAF 360
           HELLELAASFQSPSG SATRILSSSRRVRGALRNAEKPQFVSAFCQSNCGDVSPNVLGAF
Sbjct: 301 HELLELAASFQSPSGRSATRILSSSRRVRGALRNAEKPQFVSAFCQSNCGDVSPNVLGAF 360

Query: 361 CIDTGLSCDFNHSTCNGKNELCYGRGPGYPDEFESTRIIGKRQFRKAVDLFSNASEQLQG 420
           CIDTGL CDFNHSTCNGKNELCYGRGPGYPDEFESTRIIGKRQFRKAV LFSNASEQLQG
Sbjct: 361 CIDTGLPCDFNHSTCNGKNELCYGRGPGYPDEFESTRIIGKRQFRKAVALFSNASEQLQG 420

Query: 421 KVDYRHSYVDFSKLEVAIECGGTTEVVQTCPAAMGYSFAAGTTDGPGAFDFEQGDDKGNP 480
           KVDYRHSYVDFSKLEV IE GGTTEVVQTCPAAMGYSFAAGTTDGPGAFDFEQGDDKGNP
Sbjct: 421 KVDYRHSYVDFSKLEVTIERGGTTEVVQTCPAAMGYSFAAGTTDGPGAFDFEQGDDKGNP 480

Query: 481 FWKHIRNFLKKPKKEQIDCQQPKPILLDTGEMTKPYYWAPSILPIQILRIGQLIILGVPG 540
           FWKHIRNFLKKPKKEQIDCQ PKPILLDTGEMTKPYYWAPSILPIQILR+GQLIILGVPG
Sbjct: 481 FWKHIRNFLKKPKKEQIDCQHPKPILLDTGEMTKPYYWAPSILPIQILRVGQLIILGVPG 540

Query: 541 EFSTMAGRRLRDAVQTVLTDGDKSEFSGDIHIVIAGLTNSYSQYVTTFEEYQVQRYEGAS 600
           EFSTMAGRRLRDAV+TVLTDGDKSEFS DIHIVIAGLTNSYSQYVTTFEEYQVQRYEGAS
Sbjct: 541 EFSTMAGRRLRDAVKTVLTDGDKSEFSDDIHIVIAGLTNSYSQYVTTFEEYQVQRYEGAS 600

Query: 601 TLYGPHTLSAYIQEFKKLARALVDGQAVGAGPQPPDLLDKQISFLPSVVMDTTPHSVKFG 660
           TLYGPHTLSAYIQEFKKLAR+LVDGQAV AGPQPPDLLDKQISFLPSVVMDTTPHSVKFG
Sbjct: 601 TLYGPHTLSAYIQEFKKLARSLVDGQAVAAGPQPPDLLDKQISFLPSVVMDTTPHSVKFG 660

Query: 661 DVKSDVPKNSTFKRGDTVTVTFWSACPRNDLMTEGTYALVEILHDKKLWVPAYDDDDFCL 720
           DV+SDVPKNSTFKRGDTVTVTFWSACPRNDLMTEGTYALVEILHDKKLWVPAYDDDDFCL
Sbjct: 661 DVESDVPKNSTFKRGDTVTVTFWSACPRNDLMTEGTYALVEILHDKKLWVPAYDDDDFCL 720

Query: 721 RFKWSRPFKLSSQSHATIEWRIPKSATPGVYRITHFGAAKKLFGSISHFTGSSSAFGVM 779
           RFKWSRPFKLSSQSHATIEWRIPKSATPGVYRITHFGAAK+LFGSISHFTGSSSAFGVM
Sbjct: 721 RFKWSRPFKLSSQSHATIEWRIPKSATPGVYRITHFGAAKRLFGSISHFTGSSSAFGVM 779

BLAST of PI0000555 vs. ExPASy TrEMBL
Match: A0A1S3CL38 (Neutral ceramidase OS=Cucumis melo OX=3656 GN=LOC103502048 PE=3 SV=1)

HSP 1 Score: 1524.6 bits (3946), Expect = 0.0e+00
Identity = 760/802 (94.76%), Postives = 768/802 (95.76%), Query Frame = 0

Query: 1   MEFVSLFNFSIRRPCEALFWIFAFLLLQNSE-SSSSDSNYLIGLGSYDITGPAADVNMMG 60
           MEFVSLFNFSIRRPCEALFWIFAFLLLQNSE SSSSDSNYLIGLGSYDITGPAADVNMMG
Sbjct: 1   MEFVSLFNFSIRRPCEALFWIFAFLLLQNSESSSSSDSNYLIGLGSYDITGPAADVNMMG 60

Query: 61  YANMEQIASGVHFRLRARTFIVAEPRGERVVFVNLDACMASQLVTIKLLQRLKARYGDLY 120
           YANMEQIASGVHFRLRARTFIVAEP+GERVVFVNLDACMASQLVTIKLLQRLK RYGDLY
Sbjct: 61  YANMEQIASGVHFRLRARTFIVAEPQGERVVFVNLDACMASQLVTIKLLQRLKERYGDLY 120

Query: 121 TEKNVAISGIHTHAGPGGYLQYIIYIVTSLGFVPQSFDVLVNGIENSIIQAHENLRPGSI 180
           TEKNVAISGIHTHAGPGGYLQYIIYIVTSLGFV QSFDVLVNGIENSIIQAHENLRPGSI
Sbjct: 121 TEKNVAISGIHTHAGPGGYLQYIIYIVTSLGFVRQSFDVLVNGIENSIIQAHENLRPGSI 180

Query: 181 FINKGKLLDAGVNRSPSAYLNNPAEERGKYKYNVDKEMTLLKFVDLEWGPIGSFNWFATH 240
           FINKGKLLDAGVNRSPSAYLNNPAEERGKYKYNVDKEMTLLKFVDLEWGPIGSF+WFATH
Sbjct: 181 FINKGKLLDAGVNRSPSAYLNNPAEERGKYKYNVDKEMTLLKFVDLEWGPIGSFSWFATH 240

Query: 241 GTSMSRTNSLISGDNKGAAARFMEDWFEKKSGISSVFDISVDDRIPRRVSSIIPDLFINH 300
           GTSMSRTNSLISGDNKGAAARFMEDWFEKKSGISSVF+ISVDDRIPRRVSSIIPDLFINH
Sbjct: 241 GTSMSRTNSLISGDNKGAAARFMEDWFEKKSGISSVFNISVDDRIPRRVSSIIPDLFINH 300

Query: 301 HELLELAASFQSPSGGSATRILSSSRRVRGALRNAEKPQFVSAFCQSNCGDVSPNVLGAF 360
           HELLELAASFQSPSG SATRILSSSRRVRGALRNAEKPQFVSAFCQSNCGDVSPNVLGAF
Sbjct: 301 HELLELAASFQSPSGRSATRILSSSRRVRGALRNAEKPQFVSAFCQSNCGDVSPNVLGAF 360

Query: 361 CIDTGLSCDFNHSTCNGKNELCYGRGPGYPDEFESTRIIGKRQFRKAVDLFSNASEQLQG 420
           CIDTGL CDFNHSTCNGKNELCYGRGPGYPDEFESTRIIGKRQFRKAV LFSNASEQLQG
Sbjct: 361 CIDTGLPCDFNHSTCNGKNELCYGRGPGYPDEFESTRIIGKRQFRKAVALFSNASEQLQG 420

Query: 421 KVDYRHSYVDFSKLEVAIECGGTTEVVQTCPAAMGYSFAAGTTDGPGAFDFEQGDDK--- 480
           KVDYRHSYVDFSKLEV IE GGTTEVVQTCPAAMGYSFAAGTTDGPGAFDFEQGDDK   
Sbjct: 421 KVDYRHSYVDFSKLEVTIERGGTTEVVQTCPAAMGYSFAAGTTDGPGAFDFEQGDDKVII 480

Query: 481 --------------------GNPFWKHIRNFLKKPKKEQIDCQQPKPILLDTGEMTKPYY 540
                               GNPFWKHIRNFLKKPKKEQIDCQ PKPILLDTGEMTKPYY
Sbjct: 481 MLIRNINSISLTLAIYLIYQGNPFWKHIRNFLKKPKKEQIDCQHPKPILLDTGEMTKPYY 540

Query: 541 WAPSILPIQILRIGQLIILGVPGEFSTMAGRRLRDAVQTVLTDGDKSEFSGDIHIVIAGL 600
           WAPSILPIQILR+GQLIILGVPGEFSTMAGRRLRDAV+TVLTDGDKSEFS DIHIVIAGL
Sbjct: 541 WAPSILPIQILRVGQLIILGVPGEFSTMAGRRLRDAVKTVLTDGDKSEFSDDIHIVIAGL 600

Query: 601 TNSYSQYVTTFEEYQVQRYEGASTLYGPHTLSAYIQEFKKLARALVDGQAVGAGPQPPDL 660
           TNSYSQYVTTFEEYQVQRYEGASTLYGPHTLSAYIQEFKKLAR+LVDGQAV AGPQPPDL
Sbjct: 601 TNSYSQYVTTFEEYQVQRYEGASTLYGPHTLSAYIQEFKKLARSLVDGQAVAAGPQPPDL 660

Query: 661 LDKQISFLPSVVMDTTPHSVKFGDVKSDVPKNSTFKRGDTVTVTFWSACPRNDLMTEGTY 720
           LDKQISFLPSVVMDTTPHSVKFGDV+SDVPKNSTFKRGDTVTVTFWSACPRNDLMTEGTY
Sbjct: 661 LDKQISFLPSVVMDTTPHSVKFGDVESDVPKNSTFKRGDTVTVTFWSACPRNDLMTEGTY 720

Query: 721 ALVEILHDKKLWVPAYDDDDFCLRFKWSRPFKLSSQSHATIEWRIPKSATPGVYRITHFG 779
           ALVEILHDKKLWVPAYDDDDFCLRFKWSRPFKLSSQSHATIEWRIPKSATPGVYRITHFG
Sbjct: 721 ALVEILHDKKLWVPAYDDDDFCLRFKWSRPFKLSSQSHATIEWRIPKSATPGVYRITHFG 780

BLAST of PI0000555 vs. ExPASy TrEMBL
Match: A0A0A0KBQ0 (Neutral ceramidase OS=Cucumis sativus OX=3659 GN=Csa_6G188080 PE=3 SV=1)

HSP 1 Score: 1523.5 bits (3943), Expect = 0.0e+00
Identity = 751/778 (96.53%), Postives = 758/778 (97.43%), Query Frame = 0

Query: 1   MEFVSLFNFSIRRPCEALFWIFAFLLLQNSESSSSDSNYLIGLGSYDITGPAADVNMMGY 60
           MEFVSLFNFSIRRPCEALFWIFAFLLL+NSESSSSDSNYLIGLGSYDITGPAADVNMMGY
Sbjct: 1   MEFVSLFNFSIRRPCEALFWIFAFLLLKNSESSSSDSNYLIGLGSYDITGPAADVNMMGY 60

Query: 61  ANMEQIASGVHFRLRARTFIVAEPRGERVVFVNLDACMASQLVTIKLLQRLKARYGDLYT 120
           ANMEQIASGVHFRLRARTFIVAEP+GERVVFVNLDACMASQLVTIKLLQRLK RYGDLYT
Sbjct: 61  ANMEQIASGVHFRLRARTFIVAEPQGERVVFVNLDACMASQLVTIKLLQRLKERYGDLYT 120

Query: 121 EKNVAISGIHTHAGPGGYLQYIIYIVTSLGFVPQSFDVLVNGIENSIIQAHENLRPGSIF 180
           EKNVAISGIHTHAGPGGYLQYIIYIVTSLGFV QSFDVLVNGIENSIIQAHENLRPGSIF
Sbjct: 121 EKNVAISGIHTHAGPGGYLQYIIYIVTSLGFVRQSFDVLVNGIENSIIQAHENLRPGSIF 180

Query: 181 INKGKLLDAGVNRSPSAYLNNPAEERGKYKYNVDKEMTLLKFVDLEWGPIGSFNWFATHG 240
           INKGKLLDAGVNRSPSAYLNNPAEERGKYKYNVDKEMTLLKFVDLEWGPIGSFNWFATHG
Sbjct: 181 INKGKLLDAGVNRSPSAYLNNPAEERGKYKYNVDKEMTLLKFVDLEWGPIGSFNWFATHG 240

Query: 241 TSMSRTNSLISGDNKGAAARFMEDWFEKKSGISSVFDISVDDRIPRRVSSIIPDLFINHH 300
           TSMSRTNSLISGDNKGAAARFMEDWFEKK GISSVFD+SVDDRIPRRVSSIIPDLFI+HH
Sbjct: 241 TSMSRTNSLISGDNKGAAARFMEDWFEKKGGISSVFDMSVDDRIPRRVSSIIPDLFIDHH 300

Query: 301 ELLELAASFQSPSGGSATRILSSSRRVRGALRNAEKPQFVSAFCQSNCGDVSPNVLGAFC 360
           ELLELAASFQSPS  SATRILSSSRRVRGALRNAEKPQFVSAFCQSNCGDVSPNVLGAFC
Sbjct: 301 ELLELAASFQSPSSSSATRILSSSRRVRGALRNAEKPQFVSAFCQSNCGDVSPNVLGAFC 360

Query: 361 IDTGLSCDFNHSTCNGKNELCYGRGPGYPDEFESTRIIGKRQFRKAVDLFSNASEQLQGK 420
           IDTGL CDFNHSTCNGKNELCYGRGPGYPDEFESTR IGKRQFRKAVDLFSNASEQLQGK
Sbjct: 361 IDTGLPCDFNHSTCNGKNELCYGRGPGYPDEFESTRTIGKRQFRKAVDLFSNASEQLQGK 420

Query: 421 VDYRHSYVDFSKLEVAIECGGTTEVVQTCPAAMGYSFAAGTTDGPGAFDFEQGDDKGNPF 480
           VDYRHSYVDFSKLEV IE G T EVVQTCPAAMGYSFAAGTTDGPGAFDFEQGDDKGNPF
Sbjct: 421 VDYRHSYVDFSKLEVTIERGETNEVVQTCPAAMGYSFAAGTTDGPGAFDFEQGDDKGNPF 480

Query: 481 WKHIRNFLKKPKKEQIDCQQPKPILLDTGEMTKPYYWAPSILPIQILRIGQLIILGVPGE 540
           WKHIRNFLKKPKKEQIDCQ PKPILLDTGEMTKPY WAPSILPIQILR+GQLIILGVPGE
Sbjct: 481 WKHIRNFLKKPKKEQIDCQHPKPILLDTGEMTKPYNWAPSILPIQILRVGQLIILGVPGE 540

Query: 541 FSTMAGRRLRDAVQTVLTDGDKSEFSGDIHIVIAGLTNSYSQYVTTFEEYQVQRYEGAST 600
           FSTMAGRRLRDAV+TVLTDGDKSEFSGDIHIVIAGLTNSYSQYVTTFEEYQVQRYEGAST
Sbjct: 541 FSTMAGRRLRDAVKTVLTDGDKSEFSGDIHIVIAGLTNSYSQYVTTFEEYQVQRYEGAST 600

Query: 601 LYGPHTLSAYIQEFKKLARALVDGQAVGAGPQPPDLLDKQISFLPSVVMDTTPHSVKFGD 660
           LYGPHTLSAYIQEFKKLARALVDGQAV  GPQPPDLLDKQISFLPSVVMDTTPHSV FGD
Sbjct: 601 LYGPHTLSAYIQEFKKLARALVDGQAVAVGPQPPDLLDKQISFLPSVVMDTTPHSVNFGD 660

Query: 661 VKSDVPKNSTFKRGDTVTVTFWSACPRNDLMTEGTYALVEILHDKKLWVPAYDDDDFCLR 720
           VKSDVPKNSTFKR D VTVTFWSACPRNDLMTEGTYALVEILHDKKLWVPAYDDDDFCLR
Sbjct: 661 VKSDVPKNSTFKRSDMVTVTFWSACPRNDLMTEGTYALVEILHDKKLWVPAYDDDDFCLR 720

Query: 721 FKWSRPFKLSSQSHATIEWRIPKSATPGVYRITHFGAAKKLFGSISHFTGSSSAFGVM 779
           FKWSRPFKLSS SHATIEWRIPKSATPGVYRITHFGAAK+LFGSISHFTGSSSAF VM
Sbjct: 721 FKWSRPFKLSSHSHATIEWRIPKSATPGVYRITHFGAAKRLFGSISHFTGSSSAFRVM 778

BLAST of PI0000555 vs. ExPASy TrEMBL
Match: A0A1S3CKP7 (Neutral ceramidase OS=Cucumis melo OX=3656 GN=LOC103502048 PE=3 SV=1)

HSP 1 Score: 1494.6 bits (3868), Expect = 0.0e+00
Identity = 744/779 (95.51%), Postives = 752/779 (96.53%), Query Frame = 0

Query: 1   MEFVSLFNFSIRRPCEALFWIFAFLLLQNSE-SSSSDSNYLIGLGSYDITGPAADVNMMG 60
           MEFVSLFNFSIRRPCEALFWIFAFLLLQNSE SSSSDSNYLIGLGSYDITGPAADVNMMG
Sbjct: 1   MEFVSLFNFSIRRPCEALFWIFAFLLLQNSESSSSSDSNYLIGLGSYDITGPAADVNMMG 60

Query: 61  YANMEQIASGVHFRLRARTFIVAEPRGERVVFVNLDACMASQLVTIKLLQRLKARYGDLY 120
           YANMEQIASGVHFRLRARTFIVAEP+GERVVFVNLDACMASQLVTIKLLQRLK RYGDLY
Sbjct: 61  YANMEQIASGVHFRLRARTFIVAEPQGERVVFVNLDACMASQLVTIKLLQRLKERYGDLY 120

Query: 121 TEKNVAISGIHTHAGPGGYLQYIIYIVTSLGFVPQSFDVLVNGIENSIIQAHENLRPGSI 180
           TEKNVAISGIHTHAGPGGYLQYIIYIVTSLGFV QSFDVLVNGIENSIIQAHENLRPGSI
Sbjct: 121 TEKNVAISGIHTHAGPGGYLQYIIYIVTSLGFVRQSFDVLVNGIENSIIQAHENLRPGSI 180

Query: 181 FINKGKLLDAGVNRSPSAYLNNPAEERGKYKYNVDKEMTLLKFVDLEWGPIGSFNWFATH 240
           FINKGKLLDAGVNRSPSAYLNNPAEERGKYKYNVDKEMTLLKFVDLEWGPIGSF+WFATH
Sbjct: 181 FINKGKLLDAGVNRSPSAYLNNPAEERGKYKYNVDKEMTLLKFVDLEWGPIGSFSWFATH 240

Query: 241 GTSMSRTNSLISGDNKGAAARFMEDWFEKKSGISSVFDISVDDRIPRRVSSIIPDLFINH 300
           GTSMSRTNSLISGDNKGAAARFMEDWFEKKSGISSVF+ISVDDRIPRRVSSIIPDLFINH
Sbjct: 241 GTSMSRTNSLISGDNKGAAARFMEDWFEKKSGISSVFNISVDDRIPRRVSSIIPDLFINH 300

Query: 301 HELLELAASFQSPSGGSATRILSSSRRVRGALRNAEKPQFVSAFCQSNCGDVSPNVLGAF 360
           HELLELAASFQSPSG SATRILSSSRRVRGALRNAEKPQFVSAFCQSNCGDVSPNVLGAF
Sbjct: 301 HELLELAASFQSPSGRSATRILSSSRRVRGALRNAEKPQFVSAFCQSNCGDVSPNVLGAF 360

Query: 361 CIDTGLSCDFNHSTCNGKNELCYGRGPGYPDEFESTRIIGKRQFRKAVDLFSNASEQLQG 420
           CIDTGL CDFNHSTCNGKNELCYGRGPGYPDEFESTRIIGKRQFRKAV LFSNASEQLQG
Sbjct: 361 CIDTGLPCDFNHSTCNGKNELCYGRGPGYPDEFESTRIIGKRQFRKAVALFSNASEQLQG 420

Query: 421 KVDYRHSYVDFSKLEVAIECGGTTEVVQTCPAAMGYSFAAGTTDGPGAFDFEQGDDKGNP 480
           KVDYRHSYVDFSKLEV IE GGTTEVVQTCPAAMGYSFAAGTTDGPGAFDFEQGDDKGNP
Sbjct: 421 KVDYRHSYVDFSKLEVTIERGGTTEVVQTCPAAMGYSFAAGTTDGPGAFDFEQGDDKGNP 480

Query: 481 FWKHIRNFLKKPKKEQIDCQQPKPILLDTGEMTKPYYWAPSILPIQILRIGQLIILGVPG 540
           FWKHIRNFLKKPKKEQIDCQ PKPILLDTGEMTKPYYWAPSILPIQILR+GQLIILGVPG
Sbjct: 481 FWKHIRNFLKKPKKEQIDCQHPKPILLDTGEMTKPYYWAPSILPIQILRVGQLIILGVPG 540

Query: 541 EFSTMAGRRLRDAVQTVLTDGDKSEFSGDIHIVIAGLTNSYSQYVTTFEEYQVQRYEGAS 600
           EFSTMAGRRLRDAV+TVLTDGDKSEFS DIHIVIAGLTNSYSQYVTTFEEYQVQRYE   
Sbjct: 541 EFSTMAGRRLRDAVKTVLTDGDKSEFSDDIHIVIAGLTNSYSQYVTTFEEYQVQRYE--- 600

Query: 601 TLYGPHTLSAYIQEFKKLARALVDGQAVGAGPQPPDLLDKQISFLPSVVMDTTPHSVKFG 660
                        EFKKLAR+LVDGQAV AGPQPPDLLDKQISFLPSVVMDTTPHSVKFG
Sbjct: 601 -------------EFKKLARSLVDGQAVAAGPQPPDLLDKQISFLPSVVMDTTPHSVKFG 660

Query: 661 DVKSDVPKNSTFKRGDTVTVTFWSACPRNDLMTEGTYALVEILHDKKLWVPAYDDDDFCL 720
           DV+SDVPKNSTFKRGDTVTVTFWSACPRNDLMTEGTYALVEILHDKKLWVPAYDDDDFCL
Sbjct: 661 DVESDVPKNSTFKRGDTVTVTFWSACPRNDLMTEGTYALVEILHDKKLWVPAYDDDDFCL 720

Query: 721 RFKWSRPFKLSSQSHATIEWRIPKSATPGVYRITHFGAAKKLFGSISHFTGSSSAFGVM 779
           RFKWSRPFKLSSQSHATIEWRIPKSATPGVYRITHFGAAK+LFGSISHFTGSSSAFGVM
Sbjct: 721 RFKWSRPFKLSSQSHATIEWRIPKSATPGVYRITHFGAAKRLFGSISHFTGSSSAFGVM 763

BLAST of PI0000555 vs. NCBI nr
Match: XP_008464072.1 (PREDICTED: neutral ceramidase-like isoform X2 [Cucumis melo] >TYK15395.1 neutral ceramidase-like isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 1537.7 bits (3980), Expect = 0.0e+00
Identity = 760/779 (97.56%), Postives = 768/779 (98.59%), Query Frame = 0

Query: 1   MEFVSLFNFSIRRPCEALFWIFAFLLLQNSE-SSSSDSNYLIGLGSYDITGPAADVNMMG 60
           MEFVSLFNFSIRRPCEALFWIFAFLLLQNSE SSSSDSNYLIGLGSYDITGPAADVNMMG
Sbjct: 1   MEFVSLFNFSIRRPCEALFWIFAFLLLQNSESSSSSDSNYLIGLGSYDITGPAADVNMMG 60

Query: 61  YANMEQIASGVHFRLRARTFIVAEPRGERVVFVNLDACMASQLVTIKLLQRLKARYGDLY 120
           YANMEQIASGVHFRLRARTFIVAEP+GERVVFVNLDACMASQLVTIKLLQRLK RYGDLY
Sbjct: 61  YANMEQIASGVHFRLRARTFIVAEPQGERVVFVNLDACMASQLVTIKLLQRLKERYGDLY 120

Query: 121 TEKNVAISGIHTHAGPGGYLQYIIYIVTSLGFVPQSFDVLVNGIENSIIQAHENLRPGSI 180
           TEKNVAISGIHTHAGPGGYLQYIIYIVTSLGFV QSFDVLVNGIENSIIQAHENLRPGSI
Sbjct: 121 TEKNVAISGIHTHAGPGGYLQYIIYIVTSLGFVRQSFDVLVNGIENSIIQAHENLRPGSI 180

Query: 181 FINKGKLLDAGVNRSPSAYLNNPAEERGKYKYNVDKEMTLLKFVDLEWGPIGSFNWFATH 240
           FINKGKLLDAGVNRSPSAYLNNPAEERGKYKYNVDKEMTLLKFVDLEWGPIGSF+WFATH
Sbjct: 181 FINKGKLLDAGVNRSPSAYLNNPAEERGKYKYNVDKEMTLLKFVDLEWGPIGSFSWFATH 240

Query: 241 GTSMSRTNSLISGDNKGAAARFMEDWFEKKSGISSVFDISVDDRIPRRVSSIIPDLFINH 300
           GTSMSRTNSLISGDNKGAAARFMEDWFEKKSGISSVF+ISVDDRIPRRVSSIIPDLFINH
Sbjct: 241 GTSMSRTNSLISGDNKGAAARFMEDWFEKKSGISSVFNISVDDRIPRRVSSIIPDLFINH 300

Query: 301 HELLELAASFQSPSGGSATRILSSSRRVRGALRNAEKPQFVSAFCQSNCGDVSPNVLGAF 360
           HELLELAASFQSPSG SATRILSSSRRVRGALRNAEKPQFVSAFCQSNCGDVSPNVLGAF
Sbjct: 301 HELLELAASFQSPSGRSATRILSSSRRVRGALRNAEKPQFVSAFCQSNCGDVSPNVLGAF 360

Query: 361 CIDTGLSCDFNHSTCNGKNELCYGRGPGYPDEFESTRIIGKRQFRKAVDLFSNASEQLQG 420
           CIDTGL CDFNHSTCNGKNELCYGRGPGYPDEFESTRIIGKRQFRKAV LFSNASEQLQG
Sbjct: 361 CIDTGLPCDFNHSTCNGKNELCYGRGPGYPDEFESTRIIGKRQFRKAVALFSNASEQLQG 420

Query: 421 KVDYRHSYVDFSKLEVAIECGGTTEVVQTCPAAMGYSFAAGTTDGPGAFDFEQGDDKGNP 480
           KVDYRHSYVDFSKLEV IE GGTTEVVQTCPAAMGYSFAAGTTDGPGAFDFEQGDDKGNP
Sbjct: 421 KVDYRHSYVDFSKLEVTIERGGTTEVVQTCPAAMGYSFAAGTTDGPGAFDFEQGDDKGNP 480

Query: 481 FWKHIRNFLKKPKKEQIDCQQPKPILLDTGEMTKPYYWAPSILPIQILRIGQLIILGVPG 540
           FWKHIRNFLKKPKKEQIDCQ PKPILLDTGEMTKPYYWAPSILPIQILR+GQLIILGVPG
Sbjct: 481 FWKHIRNFLKKPKKEQIDCQHPKPILLDTGEMTKPYYWAPSILPIQILRVGQLIILGVPG 540

Query: 541 EFSTMAGRRLRDAVQTVLTDGDKSEFSGDIHIVIAGLTNSYSQYVTTFEEYQVQRYEGAS 600
           EFSTMAGRRLRDAV+TVLTDGDKSEFS DIHIVIAGLTNSYSQYVTTFEEYQVQRYEGAS
Sbjct: 541 EFSTMAGRRLRDAVKTVLTDGDKSEFSDDIHIVIAGLTNSYSQYVTTFEEYQVQRYEGAS 600

Query: 601 TLYGPHTLSAYIQEFKKLARALVDGQAVGAGPQPPDLLDKQISFLPSVVMDTTPHSVKFG 660
           TLYGPHTLSAYIQEFKKLAR+LVDGQAV AGPQPPDLLDKQISFLPSVVMDTTPHSVKFG
Sbjct: 601 TLYGPHTLSAYIQEFKKLARSLVDGQAVAAGPQPPDLLDKQISFLPSVVMDTTPHSVKFG 660

Query: 661 DVKSDVPKNSTFKRGDTVTVTFWSACPRNDLMTEGTYALVEILHDKKLWVPAYDDDDFCL 720
           DV+SDVPKNSTFKRGDTVTVTFWSACPRNDLMTEGTYALVEILHDKKLWVPAYDDDDFCL
Sbjct: 661 DVESDVPKNSTFKRGDTVTVTFWSACPRNDLMTEGTYALVEILHDKKLWVPAYDDDDFCL 720

Query: 721 RFKWSRPFKLSSQSHATIEWRIPKSATPGVYRITHFGAAKKLFGSISHFTGSSSAFGVM 779
           RFKWSRPFKLSSQSHATIEWRIPKSATPGVYRITHFGAAK+LFGSISHFTGSSSAFGVM
Sbjct: 721 RFKWSRPFKLSSQSHATIEWRIPKSATPGVYRITHFGAAKRLFGSISHFTGSSSAFGVM 779

BLAST of PI0000555 vs. NCBI nr
Match: XP_008464071.1 (PREDICTED: neutral ceramidase-like isoform X1 [Cucumis melo])

HSP 1 Score: 1524.6 bits (3946), Expect = 0.0e+00
Identity = 760/802 (94.76%), Postives = 768/802 (95.76%), Query Frame = 0

Query: 1   MEFVSLFNFSIRRPCEALFWIFAFLLLQNSE-SSSSDSNYLIGLGSYDITGPAADVNMMG 60
           MEFVSLFNFSIRRPCEALFWIFAFLLLQNSE SSSSDSNYLIGLGSYDITGPAADVNMMG
Sbjct: 1   MEFVSLFNFSIRRPCEALFWIFAFLLLQNSESSSSSDSNYLIGLGSYDITGPAADVNMMG 60

Query: 61  YANMEQIASGVHFRLRARTFIVAEPRGERVVFVNLDACMASQLVTIKLLQRLKARYGDLY 120
           YANMEQIASGVHFRLRARTFIVAEP+GERVVFVNLDACMASQLVTIKLLQRLK RYGDLY
Sbjct: 61  YANMEQIASGVHFRLRARTFIVAEPQGERVVFVNLDACMASQLVTIKLLQRLKERYGDLY 120

Query: 121 TEKNVAISGIHTHAGPGGYLQYIIYIVTSLGFVPQSFDVLVNGIENSIIQAHENLRPGSI 180
           TEKNVAISGIHTHAGPGGYLQYIIYIVTSLGFV QSFDVLVNGIENSIIQAHENLRPGSI
Sbjct: 121 TEKNVAISGIHTHAGPGGYLQYIIYIVTSLGFVRQSFDVLVNGIENSIIQAHENLRPGSI 180

Query: 181 FINKGKLLDAGVNRSPSAYLNNPAEERGKYKYNVDKEMTLLKFVDLEWGPIGSFNWFATH 240
           FINKGKLLDAGVNRSPSAYLNNPAEERGKYKYNVDKEMTLLKFVDLEWGPIGSF+WFATH
Sbjct: 181 FINKGKLLDAGVNRSPSAYLNNPAEERGKYKYNVDKEMTLLKFVDLEWGPIGSFSWFATH 240

Query: 241 GTSMSRTNSLISGDNKGAAARFMEDWFEKKSGISSVFDISVDDRIPRRVSSIIPDLFINH 300
           GTSMSRTNSLISGDNKGAAARFMEDWFEKKSGISSVF+ISVDDRIPRRVSSIIPDLFINH
Sbjct: 241 GTSMSRTNSLISGDNKGAAARFMEDWFEKKSGISSVFNISVDDRIPRRVSSIIPDLFINH 300

Query: 301 HELLELAASFQSPSGGSATRILSSSRRVRGALRNAEKPQFVSAFCQSNCGDVSPNVLGAF 360
           HELLELAASFQSPSG SATRILSSSRRVRGALRNAEKPQFVSAFCQSNCGDVSPNVLGAF
Sbjct: 301 HELLELAASFQSPSGRSATRILSSSRRVRGALRNAEKPQFVSAFCQSNCGDVSPNVLGAF 360

Query: 361 CIDTGLSCDFNHSTCNGKNELCYGRGPGYPDEFESTRIIGKRQFRKAVDLFSNASEQLQG 420
           CIDTGL CDFNHSTCNGKNELCYGRGPGYPDEFESTRIIGKRQFRKAV LFSNASEQLQG
Sbjct: 361 CIDTGLPCDFNHSTCNGKNELCYGRGPGYPDEFESTRIIGKRQFRKAVALFSNASEQLQG 420

Query: 421 KVDYRHSYVDFSKLEVAIECGGTTEVVQTCPAAMGYSFAAGTTDGPGAFDFEQGDDK--- 480
           KVDYRHSYVDFSKLEV IE GGTTEVVQTCPAAMGYSFAAGTTDGPGAFDFEQGDDK   
Sbjct: 421 KVDYRHSYVDFSKLEVTIERGGTTEVVQTCPAAMGYSFAAGTTDGPGAFDFEQGDDKVII 480

Query: 481 --------------------GNPFWKHIRNFLKKPKKEQIDCQQPKPILLDTGEMTKPYY 540
                               GNPFWKHIRNFLKKPKKEQIDCQ PKPILLDTGEMTKPYY
Sbjct: 481 MLIRNINSISLTLAIYLIYQGNPFWKHIRNFLKKPKKEQIDCQHPKPILLDTGEMTKPYY 540

Query: 541 WAPSILPIQILRIGQLIILGVPGEFSTMAGRRLRDAVQTVLTDGDKSEFSGDIHIVIAGL 600
           WAPSILPIQILR+GQLIILGVPGEFSTMAGRRLRDAV+TVLTDGDKSEFS DIHIVIAGL
Sbjct: 541 WAPSILPIQILRVGQLIILGVPGEFSTMAGRRLRDAVKTVLTDGDKSEFSDDIHIVIAGL 600

Query: 601 TNSYSQYVTTFEEYQVQRYEGASTLYGPHTLSAYIQEFKKLARALVDGQAVGAGPQPPDL 660
           TNSYSQYVTTFEEYQVQRYEGASTLYGPHTLSAYIQEFKKLAR+LVDGQAV AGPQPPDL
Sbjct: 601 TNSYSQYVTTFEEYQVQRYEGASTLYGPHTLSAYIQEFKKLARSLVDGQAVAAGPQPPDL 660

Query: 661 LDKQISFLPSVVMDTTPHSVKFGDVKSDVPKNSTFKRGDTVTVTFWSACPRNDLMTEGTY 720
           LDKQISFLPSVVMDTTPHSVKFGDV+SDVPKNSTFKRGDTVTVTFWSACPRNDLMTEGTY
Sbjct: 661 LDKQISFLPSVVMDTTPHSVKFGDVESDVPKNSTFKRGDTVTVTFWSACPRNDLMTEGTY 720

Query: 721 ALVEILHDKKLWVPAYDDDDFCLRFKWSRPFKLSSQSHATIEWRIPKSATPGVYRITHFG 779
           ALVEILHDKKLWVPAYDDDDFCLRFKWSRPFKLSSQSHATIEWRIPKSATPGVYRITHFG
Sbjct: 721 ALVEILHDKKLWVPAYDDDDFCLRFKWSRPFKLSSQSHATIEWRIPKSATPGVYRITHFG 780

BLAST of PI0000555 vs. NCBI nr
Match: XP_004143138.1 (neutral ceramidase 1 isoform X2 [Cucumis sativus] >KGN47115.1 hypothetical protein Csa_021076 [Cucumis sativus])

HSP 1 Score: 1523.5 bits (3943), Expect = 0.0e+00
Identity = 751/778 (96.53%), Postives = 758/778 (97.43%), Query Frame = 0

Query: 1   MEFVSLFNFSIRRPCEALFWIFAFLLLQNSESSSSDSNYLIGLGSYDITGPAADVNMMGY 60
           MEFVSLFNFSIRRPCEALFWIFAFLLL+NSESSSSDSNYLIGLGSYDITGPAADVNMMGY
Sbjct: 1   MEFVSLFNFSIRRPCEALFWIFAFLLLKNSESSSSDSNYLIGLGSYDITGPAADVNMMGY 60

Query: 61  ANMEQIASGVHFRLRARTFIVAEPRGERVVFVNLDACMASQLVTIKLLQRLKARYGDLYT 120
           ANMEQIASGVHFRLRARTFIVAEP+GERVVFVNLDACMASQLVTIKLLQRLK RYGDLYT
Sbjct: 61  ANMEQIASGVHFRLRARTFIVAEPQGERVVFVNLDACMASQLVTIKLLQRLKERYGDLYT 120

Query: 121 EKNVAISGIHTHAGPGGYLQYIIYIVTSLGFVPQSFDVLVNGIENSIIQAHENLRPGSIF 180
           EKNVAISGIHTHAGPGGYLQYIIYIVTSLGFV QSFDVLVNGIENSIIQAHENLRPGSIF
Sbjct: 121 EKNVAISGIHTHAGPGGYLQYIIYIVTSLGFVRQSFDVLVNGIENSIIQAHENLRPGSIF 180

Query: 181 INKGKLLDAGVNRSPSAYLNNPAEERGKYKYNVDKEMTLLKFVDLEWGPIGSFNWFATHG 240
           INKGKLLDAGVNRSPSAYLNNPAEERGKYKYNVDKEMTLLKFVDLEWGPIGSFNWFATHG
Sbjct: 181 INKGKLLDAGVNRSPSAYLNNPAEERGKYKYNVDKEMTLLKFVDLEWGPIGSFNWFATHG 240

Query: 241 TSMSRTNSLISGDNKGAAARFMEDWFEKKSGISSVFDISVDDRIPRRVSSIIPDLFINHH 300
           TSMSRTNSLISGDNKGAAARFMEDWFEKK GISSVFD+SVDDRIPRRVSSIIPDLFI+HH
Sbjct: 241 TSMSRTNSLISGDNKGAAARFMEDWFEKKGGISSVFDMSVDDRIPRRVSSIIPDLFIDHH 300

Query: 301 ELLELAASFQSPSGGSATRILSSSRRVRGALRNAEKPQFVSAFCQSNCGDVSPNVLGAFC 360
           ELLELAASFQSPS  SATRILSSSRRVRGALRNAEKPQFVSAFCQSNCGDVSPNVLGAFC
Sbjct: 301 ELLELAASFQSPSSSSATRILSSSRRVRGALRNAEKPQFVSAFCQSNCGDVSPNVLGAFC 360

Query: 361 IDTGLSCDFNHSTCNGKNELCYGRGPGYPDEFESTRIIGKRQFRKAVDLFSNASEQLQGK 420
           IDTGL CDFNHSTCNGKNELCYGRGPGYPDEFESTR IGKRQFRKAVDLFSNASEQLQGK
Sbjct: 361 IDTGLPCDFNHSTCNGKNELCYGRGPGYPDEFESTRTIGKRQFRKAVDLFSNASEQLQGK 420

Query: 421 VDYRHSYVDFSKLEVAIECGGTTEVVQTCPAAMGYSFAAGTTDGPGAFDFEQGDDKGNPF 480
           VDYRHSYVDFSKLEV IE G T EVVQTCPAAMGYSFAAGTTDGPGAFDFEQGDDKGNPF
Sbjct: 421 VDYRHSYVDFSKLEVTIERGETNEVVQTCPAAMGYSFAAGTTDGPGAFDFEQGDDKGNPF 480

Query: 481 WKHIRNFLKKPKKEQIDCQQPKPILLDTGEMTKPYYWAPSILPIQILRIGQLIILGVPGE 540
           WKHIRNFLKKPKKEQIDCQ PKPILLDTGEMTKPY WAPSILPIQILR+GQLIILGVPGE
Sbjct: 481 WKHIRNFLKKPKKEQIDCQHPKPILLDTGEMTKPYNWAPSILPIQILRVGQLIILGVPGE 540

Query: 541 FSTMAGRRLRDAVQTVLTDGDKSEFSGDIHIVIAGLTNSYSQYVTTFEEYQVQRYEGAST 600
           FSTMAGRRLRDAV+TVLTDGDKSEFSGDIHIVIAGLTNSYSQYVTTFEEYQVQRYEGAST
Sbjct: 541 FSTMAGRRLRDAVKTVLTDGDKSEFSGDIHIVIAGLTNSYSQYVTTFEEYQVQRYEGAST 600

Query: 601 LYGPHTLSAYIQEFKKLARALVDGQAVGAGPQPPDLLDKQISFLPSVVMDTTPHSVKFGD 660
           LYGPHTLSAYIQEFKKLARALVDGQAV  GPQPPDLLDKQISFLPSVVMDTTPHSV FGD
Sbjct: 601 LYGPHTLSAYIQEFKKLARALVDGQAVAVGPQPPDLLDKQISFLPSVVMDTTPHSVNFGD 660

Query: 661 VKSDVPKNSTFKRGDTVTVTFWSACPRNDLMTEGTYALVEILHDKKLWVPAYDDDDFCLR 720
           VKSDVPKNSTFKR D VTVTFWSACPRNDLMTEGTYALVEILHDKKLWVPAYDDDDFCLR
Sbjct: 661 VKSDVPKNSTFKRSDMVTVTFWSACPRNDLMTEGTYALVEILHDKKLWVPAYDDDDFCLR 720

Query: 721 FKWSRPFKLSSQSHATIEWRIPKSATPGVYRITHFGAAKKLFGSISHFTGSSSAFGVM 779
           FKWSRPFKLSS SHATIEWRIPKSATPGVYRITHFGAAK+LFGSISHFTGSSSAF VM
Sbjct: 721 FKWSRPFKLSSHSHATIEWRIPKSATPGVYRITHFGAAKRLFGSISHFTGSSSAFRVM 778

BLAST of PI0000555 vs. NCBI nr
Match: XP_011657138.1 (neutral ceramidase 1 isoform X1 [Cucumis sativus])

HSP 1 Score: 1510.4 bits (3909), Expect = 0.0e+00
Identity = 751/801 (93.76%), Postives = 758/801 (94.63%), Query Frame = 0

Query: 1   MEFVSLFNFSIRRPCEALFWIFAFLLLQNSESSSSDSNYLIGLGSYDITGPAADVNMMGY 60
           MEFVSLFNFSIRRPCEALFWIFAFLLL+NSESSSSDSNYLIGLGSYDITGPAADVNMMGY
Sbjct: 1   MEFVSLFNFSIRRPCEALFWIFAFLLLKNSESSSSDSNYLIGLGSYDITGPAADVNMMGY 60

Query: 61  ANMEQIASGVHFRLRARTFIVAEPRGERVVFVNLDACMASQLVTIKLLQRLKARYGDLYT 120
           ANMEQIASGVHFRLRARTFIVAEP+GERVVFVNLDACMASQLVTIKLLQRLK RYGDLYT
Sbjct: 61  ANMEQIASGVHFRLRARTFIVAEPQGERVVFVNLDACMASQLVTIKLLQRLKERYGDLYT 120

Query: 121 EKNVAISGIHTHAGPGGYLQYIIYIVTSLGFVPQSFDVLVNGIENSIIQAHENLRPGSIF 180
           EKNVAISGIHTHAGPGGYLQYIIYIVTSLGFV QSFDVLVNGIENSIIQAHENLRPGSIF
Sbjct: 121 EKNVAISGIHTHAGPGGYLQYIIYIVTSLGFVRQSFDVLVNGIENSIIQAHENLRPGSIF 180

Query: 181 INKGKLLDAGVNRSPSAYLNNPAEERGKYKYNVDKEMTLLKFVDLEWGPIGSFNWFATHG 240
           INKGKLLDAGVNRSPSAYLNNPAEERGKYKYNVDKEMTLLKFVDLEWGPIGSFNWFATHG
Sbjct: 181 INKGKLLDAGVNRSPSAYLNNPAEERGKYKYNVDKEMTLLKFVDLEWGPIGSFNWFATHG 240

Query: 241 TSMSRTNSLISGDNKGAAARFMEDWFEKKSGISSVFDISVDDRIPRRVSSIIPDLFINHH 300
           TSMSRTNSLISGDNKGAAARFMEDWFEKK GISSVFD+SVDDRIPRRVSSIIPDLFI+HH
Sbjct: 241 TSMSRTNSLISGDNKGAAARFMEDWFEKKGGISSVFDMSVDDRIPRRVSSIIPDLFIDHH 300

Query: 301 ELLELAASFQSPSGGSATRILSSSRRVRGALRNAEKPQFVSAFCQSNCGDVSPNVLGAFC 360
           ELLELAASFQSPS  SATRILSSSRRVRGALRNAEKPQFVSAFCQSNCGDVSPNVLGAFC
Sbjct: 301 ELLELAASFQSPSSSSATRILSSSRRVRGALRNAEKPQFVSAFCQSNCGDVSPNVLGAFC 360

Query: 361 IDTGLSCDFNHSTCNGKNELCYGRGPGYPDEFESTRIIGKRQFRKAVDLFSNASEQLQGK 420
           IDTGL CDFNHSTCNGKNELCYGRGPGYPDEFESTR IGKRQFRKAVDLFSNASEQLQGK
Sbjct: 361 IDTGLPCDFNHSTCNGKNELCYGRGPGYPDEFESTRTIGKRQFRKAVDLFSNASEQLQGK 420

Query: 421 VDYRHSYVDFSKLEVAIECGGTTEVVQTCPAAMGYSFAAGTTDGPGAFDFEQGDDK---- 480
           VDYRHSYVDFSKLEV IE G T EVVQTCPAAMGYSFAAGTTDGPGAFDFEQGDDK    
Sbjct: 421 VDYRHSYVDFSKLEVTIERGETNEVVQTCPAAMGYSFAAGTTDGPGAFDFEQGDDKVIIT 480

Query: 481 -------------------GNPFWKHIRNFLKKPKKEQIDCQQPKPILLDTGEMTKPYYW 540
                              GNPFWKHIRNFLKKPKKEQIDCQ PKPILLDTGEMTKPY W
Sbjct: 481 LIRNVNSISLTLAISLIYQGNPFWKHIRNFLKKPKKEQIDCQHPKPILLDTGEMTKPYNW 540

Query: 541 APSILPIQILRIGQLIILGVPGEFSTMAGRRLRDAVQTVLTDGDKSEFSGDIHIVIAGLT 600
           APSILPIQILR+GQLIILGVPGEFSTMAGRRLRDAV+TVLTDGDKSEFSGDIHIVIAGLT
Sbjct: 541 APSILPIQILRVGQLIILGVPGEFSTMAGRRLRDAVKTVLTDGDKSEFSGDIHIVIAGLT 600

Query: 601 NSYSQYVTTFEEYQVQRYEGASTLYGPHTLSAYIQEFKKLARALVDGQAVGAGPQPPDLL 660
           NSYSQYVTTFEEYQVQRYEGASTLYGPHTLSAYIQEFKKLARALVDGQAV  GPQPPDLL
Sbjct: 601 NSYSQYVTTFEEYQVQRYEGASTLYGPHTLSAYIQEFKKLARALVDGQAVAVGPQPPDLL 660

Query: 661 DKQISFLPSVVMDTTPHSVKFGDVKSDVPKNSTFKRGDTVTVTFWSACPRNDLMTEGTYA 720
           DKQISFLPSVVMDTTPHSV FGDVKSDVPKNSTFKR D VTVTFWSACPRNDLMTEGTYA
Sbjct: 661 DKQISFLPSVVMDTTPHSVNFGDVKSDVPKNSTFKRSDMVTVTFWSACPRNDLMTEGTYA 720

Query: 721 LVEILHDKKLWVPAYDDDDFCLRFKWSRPFKLSSQSHATIEWRIPKSATPGVYRITHFGA 779
           LVEILHDKKLWVPAYDDDDFCLRFKWSRPFKLSS SHATIEWRIPKSATPGVYRITHFGA
Sbjct: 721 LVEILHDKKLWVPAYDDDDFCLRFKWSRPFKLSSHSHATIEWRIPKSATPGVYRITHFGA 780

BLAST of PI0000555 vs. NCBI nr
Match: XP_008464073.1 (PREDICTED: neutral ceramidase-like isoform X3 [Cucumis melo])

HSP 1 Score: 1494.6 bits (3868), Expect = 0.0e+00
Identity = 744/779 (95.51%), Postives = 752/779 (96.53%), Query Frame = 0

Query: 1   MEFVSLFNFSIRRPCEALFWIFAFLLLQNSE-SSSSDSNYLIGLGSYDITGPAADVNMMG 60
           MEFVSLFNFSIRRPCEALFWIFAFLLLQNSE SSSSDSNYLIGLGSYDITGPAADVNMMG
Sbjct: 1   MEFVSLFNFSIRRPCEALFWIFAFLLLQNSESSSSSDSNYLIGLGSYDITGPAADVNMMG 60

Query: 61  YANMEQIASGVHFRLRARTFIVAEPRGERVVFVNLDACMASQLVTIKLLQRLKARYGDLY 120
           YANMEQIASGVHFRLRARTFIVAEP+GERVVFVNLDACMASQLVTIKLLQRLK RYGDLY
Sbjct: 61  YANMEQIASGVHFRLRARTFIVAEPQGERVVFVNLDACMASQLVTIKLLQRLKERYGDLY 120

Query: 121 TEKNVAISGIHTHAGPGGYLQYIIYIVTSLGFVPQSFDVLVNGIENSIIQAHENLRPGSI 180
           TEKNVAISGIHTHAGPGGYLQYIIYIVTSLGFV QSFDVLVNGIENSIIQAHENLRPGSI
Sbjct: 121 TEKNVAISGIHTHAGPGGYLQYIIYIVTSLGFVRQSFDVLVNGIENSIIQAHENLRPGSI 180

Query: 181 FINKGKLLDAGVNRSPSAYLNNPAEERGKYKYNVDKEMTLLKFVDLEWGPIGSFNWFATH 240
           FINKGKLLDAGVNRSPSAYLNNPAEERGKYKYNVDKEMTLLKFVDLEWGPIGSF+WFATH
Sbjct: 181 FINKGKLLDAGVNRSPSAYLNNPAEERGKYKYNVDKEMTLLKFVDLEWGPIGSFSWFATH 240

Query: 241 GTSMSRTNSLISGDNKGAAARFMEDWFEKKSGISSVFDISVDDRIPRRVSSIIPDLFINH 300
           GTSMSRTNSLISGDNKGAAARFMEDWFEKKSGISSVF+ISVDDRIPRRVSSIIPDLFINH
Sbjct: 241 GTSMSRTNSLISGDNKGAAARFMEDWFEKKSGISSVFNISVDDRIPRRVSSIIPDLFINH 300

Query: 301 HELLELAASFQSPSGGSATRILSSSRRVRGALRNAEKPQFVSAFCQSNCGDVSPNVLGAF 360
           HELLELAASFQSPSG SATRILSSSRRVRGALRNAEKPQFVSAFCQSNCGDVSPNVLGAF
Sbjct: 301 HELLELAASFQSPSGRSATRILSSSRRVRGALRNAEKPQFVSAFCQSNCGDVSPNVLGAF 360

Query: 361 CIDTGLSCDFNHSTCNGKNELCYGRGPGYPDEFESTRIIGKRQFRKAVDLFSNASEQLQG 420
           CIDTGL CDFNHSTCNGKNELCYGRGPGYPDEFESTRIIGKRQFRKAV LFSNASEQLQG
Sbjct: 361 CIDTGLPCDFNHSTCNGKNELCYGRGPGYPDEFESTRIIGKRQFRKAVALFSNASEQLQG 420

Query: 421 KVDYRHSYVDFSKLEVAIECGGTTEVVQTCPAAMGYSFAAGTTDGPGAFDFEQGDDKGNP 480
           KVDYRHSYVDFSKLEV IE GGTTEVVQTCPAAMGYSFAAGTTDGPGAFDFEQGDDKGNP
Sbjct: 421 KVDYRHSYVDFSKLEVTIERGGTTEVVQTCPAAMGYSFAAGTTDGPGAFDFEQGDDKGNP 480

Query: 481 FWKHIRNFLKKPKKEQIDCQQPKPILLDTGEMTKPYYWAPSILPIQILRIGQLIILGVPG 540
           FWKHIRNFLKKPKKEQIDCQ PKPILLDTGEMTKPYYWAPSILPIQILR+GQLIILGVPG
Sbjct: 481 FWKHIRNFLKKPKKEQIDCQHPKPILLDTGEMTKPYYWAPSILPIQILRVGQLIILGVPG 540

Query: 541 EFSTMAGRRLRDAVQTVLTDGDKSEFSGDIHIVIAGLTNSYSQYVTTFEEYQVQRYEGAS 600
           EFSTMAGRRLRDAV+TVLTDGDKSEFS DIHIVIAGLTNSYSQYVTTFEEYQVQRYE   
Sbjct: 541 EFSTMAGRRLRDAVKTVLTDGDKSEFSDDIHIVIAGLTNSYSQYVTTFEEYQVQRYE--- 600

Query: 601 TLYGPHTLSAYIQEFKKLARALVDGQAVGAGPQPPDLLDKQISFLPSVVMDTTPHSVKFG 660
                        EFKKLAR+LVDGQAV AGPQPPDLLDKQISFLPSVVMDTTPHSVKFG
Sbjct: 601 -------------EFKKLARSLVDGQAVAAGPQPPDLLDKQISFLPSVVMDTTPHSVKFG 660

Query: 661 DVKSDVPKNSTFKRGDTVTVTFWSACPRNDLMTEGTYALVEILHDKKLWVPAYDDDDFCL 720
           DV+SDVPKNSTFKRGDTVTVTFWSACPRNDLMTEGTYALVEILHDKKLWVPAYDDDDFCL
Sbjct: 661 DVESDVPKNSTFKRGDTVTVTFWSACPRNDLMTEGTYALVEILHDKKLWVPAYDDDDFCL 720

Query: 721 RFKWSRPFKLSSQSHATIEWRIPKSATPGVYRITHFGAAKKLFGSISHFTGSSSAFGVM 779
           RFKWSRPFKLSSQSHATIEWRIPKSATPGVYRITHFGAAK+LFGSISHFTGSSSAFGVM
Sbjct: 721 RFKWSRPFKLSSQSHATIEWRIPKSATPGVYRITHFGAAKRLFGSISHFTGSSSAFGVM 763

BLAST of PI0000555 vs. TAIR 10
Match: AT1G07380.1 (Neutral/alkaline non-lysosomal ceramidase )

HSP 1 Score: 1178.3 bits (3047), Expect = 0.0e+00
Identity = 577/764 (75.52%), Postives = 653/764 (85.47%), Query Frame = 0

Query: 17  ALFWIFAFLLLQNSESSSSDSNYLIGLGSYDITGPAADVNMMGYANMEQIASGVHFRLRA 76
           + F+I   L L       S S YLIGLGSYDITGPAADVNMMGYANMEQ+ASG+HFRLRA
Sbjct: 15  SFFYISTVLFLLTGGGVYSHSEYLIGLGSYDITGPAADVNMMGYANMEQVASGIHFRLRA 74

Query: 77  RTFIVAEPRGERVVFVNLDACMASQLVTIKLLQRLKARYGDLYTEKNVAISGIHTHAGPG 136
           RTFIV+EP+G+RVVFVNLDACMASQ+V +K+++RLKARYGDLYTE+NV ISGIHTHAGPG
Sbjct: 75  RTFIVSEPQGKRVVFVNLDACMASQIVKLKVIERLKARYGDLYTEQNVGISGIHTHAGPG 134

Query: 137 GYLQYIIYIVTSLGFVPQSFDVLVNGIENSIIQAHENLRPGSIFINKGKLLDAGVNRSPS 196
           GYLQY++YIVTSLGFV QSFD LV+GIENSIIQAHENLRPGSIF+N G+LLDAGVNRSPS
Sbjct: 135 GYLQYVVYIVTSLGFVRQSFDALVDGIENSIIQAHENLRPGSIFLNNGELLDAGVNRSPS 194

Query: 197 AYLNNPAEERGKYKYNVDKEMTLLKFVDLEWGPIGSFNWFATHGTSMSRTNSLISGDNKG 256
           AYLNNP++ER K+KYNVDKEMTLLKFVD +WGP+GSFNWFATHGTSMSRTNSLISGDNKG
Sbjct: 195 AYLNNPSKERSKHKYNVDKEMTLLKFVDDQWGPVGSFNWFATHGTSMSRTNSLISGDNKG 254

Query: 257 AAARFMEDWFEKKSGISSVFDISVDDRIPRRVSSIIPDLFINHHELLELAASFQSPSGGS 316
           AA+RFMEDW+E+ +   S  +  + D IPRRVSS+I +   +HHELLELA+ F+S  G  
Sbjct: 255 AASRFMEDWYEQNTAERSYSEEFISDEIPRRVSSLIENHQDSHHELLELASYFESQPGKP 314

Query: 317 ATRILSSSRRVRGALRNAEKPQFVSAFCQSNCGDVSPNVLGAFCIDTGLSCDFNHSTCNG 376
            TRI SS+RRVR ALR A+KP FVSAFCQ+NCGDVSPNVLGAFC+DTGL CDFNHSTC G
Sbjct: 315 VTRISSSARRVRSALRKADKPGFVSAFCQTNCGDVSPNVLGAFCLDTGLPCDFNHSTCGG 374

Query: 377 KNELCYGRGPGYPDEFESTRIIGKRQFRKAVDLFSNASEQLQGKVDYRHSYVDFSKLEVA 436
           KNE+CYGRGPGYPDEFESTRIIG+RQF+ A++LF+ ASEQLQGKVDYRH YVDFS+L V 
Sbjct: 375 KNEMCYGRGPGYPDEFESTRIIGERQFKMALELFNKASEQLQGKVDYRHVYVDFSQLNVT 434

Query: 437 I-ECGGTTEVVQTCPAAMGYSFAAGTTDGPGAFDFEQGDDKGNPFWKHIRNFLKKPKKEQ 496
           + +  G +EVV+TCPAAMG++FAAGTTDGPGAFDF QGDDKGNPFW+ +RN LK P K+Q
Sbjct: 435 LPKKDGKSEVVKTCPAAMGFAFAAGTTDGPGAFDFTQGDDKGNPFWRLVRNVLKTPDKKQ 494

Query: 497 IDCQQPKPILLDTGEMTKPYYWAPSILPIQILRIGQLIILGVPGEFSTMAGRRLRDAVQT 556
           IDC  PKPILLDTGEMTKPY WAPSIL +Q+LRIGQL IL VPGEF+TMAGRRLR AV+T
Sbjct: 495 IDCHYPKPILLDTGEMTKPYDWAPSILSLQVLRIGQLFILSVPGEFTTMAGRRLRYAVKT 554

Query: 557 VLTDGDKSEFSGDIHIVIAGLTNSYSQYVTTFEEYQVQRYEGASTLYGPHTLSAYIQEFK 616
            L +    + SG+IH+VIAGL N YSQYVTTFEEYQVQRYEGASTLYGPHTLS YIQEFK
Sbjct: 555 QLKNSGNKDLSGEIHVVIAGLANGYSQYVTTFEEYQVQRYEGASTLYGPHTLSGYIQEFK 614

Query: 617 KLARALVDGQAVGAGPQPPDLLDKQISFLPSVVMDTTPHSVKFGDVKSDVPKNSTFKRGD 676
           KL+++LV    V  GPQPPDLLDKQ+SFL  V+MDTTP    FGDV SDVPKN + KRG+
Sbjct: 615 KLSKSLVLDMPVQPGPQPPDLLDKQLSFLTPVMMDTTPSGDSFGDVISDVPKNLSLKRGN 674

Query: 677 -TVTVTFWSACPRNDLMTEGTYALVEILHDK-KLWVPAYDDDDFCLRFKWSRPFKLSSQS 736
             VTV F SACPRNDL+TEGT+ LVE L  K K W P YDDDD CLRFKWSR  KLSS+S
Sbjct: 675 GQVTVVFRSACPRNDLLTEGTFTLVERLEQKDKTWTPVYDDDDLCLRFKWSRHKKLSSRS 734

Query: 737 HATIEWRIPKSATPGVYRITHFGAAKKLFGSISHFTGSSSAFGV 778
            AT+EWRIP+SA+PGVYRITHFGAAKKLFGS+ HFTGSSSAF V
Sbjct: 735 QATVEWRIPESASPGVYRITHFGAAKKLFGSVHHFTGSSSAFVV 778

BLAST of PI0000555 vs. TAIR 10
Match: AT2G38010.1 (Neutral/alkaline non-lysosomal ceramidase )

HSP 1 Score: 1121.7 bits (2900), Expect = 0.0e+00
Identity = 551/764 (72.12%), Postives = 637/764 (83.38%), Query Frame = 0

Query: 18  LFWIFAFLLLQNSESSSSDSNYLIGLGSYDITGPAADVNMMGYANMEQIASGVHFRLRAR 77
           LF    FLLL     S +   YLIG+GSYDITGPAADVNMMGYAN +QIASG+HFRLRAR
Sbjct: 7   LFQFILFLLL--LLLSRTVYAYLIGVGSYDITGPAADVNMMGYANSDQIASGIHFRLRAR 66

Query: 78  TFIVAEPRGERVVFVNLDACMASQLVTIKLLQRLKARYGDLYTEKNVAISGIHTHAGPGG 137
            FIVAEP+G RVVFVNLDACMASQ+VTIK+L+RLKARYG+LYTEKNVAISGIHTHAGPGG
Sbjct: 67  AFIVAEPQGNRVVFVNLDACMASQIVTIKVLERLKARYGELYTEKNVAISGIHTHAGPGG 126

Query: 138 YLQYIIYIVTSLGFVPQSFDVLVNGIENSIIQAHENLRPGSIFINKGKLLDAGVNRSPSA 197
           YLQY+ YIVTSLGFV QSFDV+VNGIE SI+QAHE+LRPGS F+NKG LLDAGVNRSPS+
Sbjct: 127 YLQYVTYIVTSLGFVRQSFDVVVNGIEQSIVQAHESLRPGSAFVNKGDLLDAGVNRSPSS 186

Query: 198 YLNNPAEERGKYKYNVDKEMTLLKFVDLEWGPIGSFNWFATHGTSMSRTNSLISGDNKGA 257
           YLNNPA ER KYKY+VDKEMTL+KFVD + GP GSFNWFATHGTSMSRTNSLISGDNKGA
Sbjct: 187 YLNNPAAERSKYKYDVDKEMTLVKFVDSQLGPTGSFNWFATHGTSMSRTNSLISGDNKGA 246

Query: 258 AARFMEDWFE--KKSGISSVFDISVDDRIPRRVSSIIPDLFINHHELLELAASFQSPSGG 317
           AARFMEDWFE  +K+ +SS         IPRRVS+I+ D   N   LL++AA+++S  G 
Sbjct: 247 AARFMEDWFENGQKNSVSS-------RNIPRRVSTIVSDFSRNRDRLLDIAATYKSSRGH 306

Query: 318 SATRILSSSRRVRGALRNAEKPQFVSAFCQSNCGDVSPNVLGAFCIDTGLSCDFNHSTCN 377
           S  + L    RV    RN  K +FVSAFCQSNCGDVSPN LG FCIDTGL CDFNHSTCN
Sbjct: 307 SVDKSLDVKTRV----RNGSKRKFVSAFCQSNCGDVSPNTLGTFCIDTGLPCDFNHSTCN 366

Query: 378 GKNELCYGRGPGYPDEFESTRIIGKRQFRKAVDLFSNASEQLQGKVDYRHSYVDFSKLEV 437
           G+NELCYGRGPGYPDEFESTRIIG++QF+ AV+LF+ A+E+LQGK+ Y+H+Y+DFS L+V
Sbjct: 367 GQNELCYGRGPGYPDEFESTRIIGEKQFKMAVELFNKATEKLQGKIGYQHAYLDFSNLDV 426

Query: 438 AI-ECGGTTEVVQTCPAAMGYSFAAGTTDGPGAFDFEQGDDKGNPFWKHIRNFLKKPKKE 497
            + + GG +E V+TCPAAMG+ FAAGTTDGPGAFDF+QGDD+GN FW+ +RN L+ P  E
Sbjct: 427 TVPKAGGGSETVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNVFWRLVRNVLRTPGPE 486

Query: 498 QIDCQQPKPILLDTGEMTKPYYWAPSILPIQILRIGQLIILGVPGEFSTMAGRRLRDAVQ 557
           Q+ CQ+PKPILLDTGEM +PY WAPSILPIQILRIGQL+IL VPGEF+TMAGRRLRDA++
Sbjct: 487 QVQCQKPKPILLDTGEMKEPYDWAPSILPIQILRIGQLVILSVPGEFTTMAGRRLRDAIK 546

Query: 558 TVLTDGDKSEFSGDIHIVIAGLTNSYSQYVTTFEEYQVQRYEGASTLYGPHTLSAYIQEF 617
           + L   D  EFS ++H+VIAGLTN+YSQY+ TFEEY+VQRYEGASTLYG HTL+AYIQEF
Sbjct: 547 SFLISSDPKEFSNNMHVVIAGLTNTYSQYIATFEEYEVQRYEGASTLYGRHTLTAYIQEF 606

Query: 618 KKLARALVDGQAVGAGPQPPDLLDKQISFLPSVVMDTTPHSVKFGDVKSDVPKNSTFKRG 677
           KKLA ALV+G  +  GPQPPDLLDKQIS L  VV+D+TP  VKFGDVK+DVP  STF+RG
Sbjct: 607 KKLATALVNGLTLPRGPQPPDLLDKQISLLSPVVVDSTPLGVKFGDVKADVPPKSTFRRG 666

Query: 678 DTVTVTFWSACPRNDLMTEGTYALVEILHDKKLWVPAYDDDDFCLRFKWSRPFKLSSQSH 737
             V  TFWS CPRNDLMTEG++A+VE L +   W P YDDDDF L+FKWSRP KLSS+S 
Sbjct: 667 QQVNATFWSGCPRNDLMTEGSFAVVETLREGGKWAPVYDDDDFSLKFKWSRPAKLSSESQ 726

Query: 738 ATIEWRIPKSATPGVYRITHFGAAKKLFGSISHFTGSSSAFGVM 779
           ATIEWR+P+SA  GVYRI H+GA+K LFGSIS F+GSSSAF V+
Sbjct: 727 ATIEWRVPESAVAGVYRIRHYGASKSLFGSISSFSGSSSAFVVV 757

BLAST of PI0000555 vs. TAIR 10
Match: AT2G38010.2 (Neutral/alkaline non-lysosomal ceramidase )

HSP 1 Score: 1107.4 bits (2863), Expect = 0.0e+00
Identity = 551/799 (68.96%), Postives = 639/799 (79.97%), Query Frame = 0

Query: 18  LFWIFAFLLLQNSESSSSDSNYLIGLGSYDITGPAADVNMMGYANMEQIASGVHFRLRAR 77
           LF    FLLL     S +   YLIG+GSYDITGPAADVNMMGYAN +QIASG+HFRLRAR
Sbjct: 7   LFQFILFLLL--LLLSRTVYAYLIGVGSYDITGPAADVNMMGYANSDQIASGIHFRLRAR 66

Query: 78  TFIVAEPRGERVVFVNLDACMASQLVTIKLLQRLKARYGDLYTEKNVAISGIHTHAGPGG 137
            FIVAEP+G RVVFVNLDACMASQ+VTIK+L+RLKARYG+LYTEKNVAISGIHTHAGPGG
Sbjct: 67  AFIVAEPQGNRVVFVNLDACMASQIVTIKVLERLKARYGELYTEKNVAISGIHTHAGPGG 126

Query: 138 YLQYIIYIVTSLGFVPQSFDVLVNGIENSIIQAHENLRPGSIFINKGKLLDAGVNRSPSA 197
           YLQY+ YIVTSLGFV QSFDV+VNGIE SI+QAHE+LRPGS F+NKG LLDAGVNRSPS+
Sbjct: 127 YLQYVTYIVTSLGFVRQSFDVVVNGIEQSIVQAHESLRPGSAFVNKGDLLDAGVNRSPSS 186

Query: 198 YLNNPAEERGKYKYNVDKEMTLLKFVDLEWGPIGSFNWFATHGTSMSRTNSLISGDNKGA 257
           YLNNPA ER KYKY+VDKEMTL+KFVD + GP GSFNWFATHGTSMSRTNSLISGDNKGA
Sbjct: 187 YLNNPAAERSKYKYDVDKEMTLVKFVDSQLGPTGSFNWFATHGTSMSRTNSLISGDNKGA 246

Query: 258 AARFMEDWFE--KKSGISSVFDISVDDRIPRRVSSIIPDL-------------------- 317
           AARFMEDWFE  +K+ +SS         IPRRVS+I+ D                     
Sbjct: 247 AARFMEDWFENGQKNSVSS-------RNIPRRVSTIVSDFSRNRESYPFLELPNVAILYI 306

Query: 318 ---------------FINHHELLELAASFQSPSGGSATRILSSSRRVRGALRNAEKPQFV 377
                          F++   LL++AA+++S  G S  + L    RV    RN  K +FV
Sbjct: 307 AVAERLMWKVMVLFYFVSESRLLDIAATYKSSRGHSVDKSLDVKTRV----RNGSKRKFV 366

Query: 378 SAFCQSNCGDVSPNVLGAFCIDTGLSCDFNHSTCNGKNELCYGRGPGYPDEFESTRIIGK 437
           SAFCQSNCGDVSPN LG FCIDTGL CDFNHSTCNG+NELCYGRGPGYPDEFESTRIIG+
Sbjct: 367 SAFCQSNCGDVSPNTLGTFCIDTGLPCDFNHSTCNGQNELCYGRGPGYPDEFESTRIIGE 426

Query: 438 RQFRKAVDLFSNASEQLQGKVDYRHSYVDFSKLEVAI-ECGGTTEVVQTCPAAMGYSFAA 497
           +QF+ AV+LF+ A+E+LQGK+ Y+H+Y+DFS L+V + + GG +E V+TCPAAMG+ FAA
Sbjct: 427 KQFKMAVELFNKATEKLQGKIGYQHAYLDFSNLDVTVPKAGGGSETVKTCPAAMGFGFAA 486

Query: 498 GTTDGPGAFDFEQGDDKGNPFWKHIRNFLKKPKKEQIDCQQPKPILLDTGEMTKPYYWAP 557
           GTTDGPGAFDF+QGDD+GN FW+ +RN L+ P  EQ+ CQ+PKPILLDTGEM +PY WAP
Sbjct: 487 GTTDGPGAFDFKQGDDQGNVFWRLVRNVLRTPGPEQVQCQKPKPILLDTGEMKEPYDWAP 546

Query: 558 SILPIQILRIGQLIILGVPGEFSTMAGRRLRDAVQTVLTDGDKSEFSGDIHIVIAGLTNS 617
           SILPIQILRIGQL+IL VPGEF+TMAGRRLRDA+++ L   D  EFS ++H+VIAGLTN+
Sbjct: 547 SILPIQILRIGQLVILSVPGEFTTMAGRRLRDAIKSFLISSDPKEFSNNMHVVIAGLTNT 606

Query: 618 YSQYVTTFEEYQVQRYEGASTLYGPHTLSAYIQEFKKLARALVDGQAVGAGPQPPDLLDK 677
           YSQY+ TFEEY+VQRYEGASTLYG HTL+AYIQEFKKLA ALV+G  +  GPQPPDLLDK
Sbjct: 607 YSQYIATFEEYEVQRYEGASTLYGRHTLTAYIQEFKKLATALVNGLTLPRGPQPPDLLDK 666

Query: 678 QISFLPSVVMDTTPHSVKFGDVKSDVPKNSTFKRGDTVTVTFWSACPRNDLMTEGTYALV 737
           QIS L  VV+D+TP  VKFGDVK+DVP  STF+RG  V  TFWS CPRNDLMTEG++A+V
Sbjct: 667 QISLLSPVVVDSTPLGVKFGDVKADVPPKSTFRRGQQVNATFWSGCPRNDLMTEGSFAVV 726

Query: 738 EILHDKKLWVPAYDDDDFCLRFKWSRPFKLSSQSHATIEWRIPKSATPGVYRITHFGAAK 779
           E L +   W P YDDDDF L+FKWSRP KLSS+S ATIEWR+P+SA  GVYRI H+GA+K
Sbjct: 727 ETLREGGKWAPVYDDDDFSLKFKWSRPAKLSSESQATIEWRVPESAVAGVYRIRHYGASK 786

BLAST of PI0000555 vs. TAIR 10
Match: AT5G58980.1 (Neutral/alkaline non-lysosomal ceramidase )

HSP 1 Score: 1047.7 bits (2708), Expect = 4.5e-306
Identity = 531/747 (71.08%), Postives = 605/747 (80.99%), Query Frame = 0

Query: 35  SDSNYLIGLGSYDITGPAADVNMMGYANMEQIASGVHFRLRARTFIVAEPRGERVVFVNL 94
           SDS+YL+GLGSYDITGPAADVNMMGYANMEQ+ASGVHFRLRAR FIVAEP  +R+ FVNL
Sbjct: 25  SDSDYLMGLGSYDITGPAADVNMMGYANMEQVASGVHFRLRARAFIVAEPYKKRIAFVNL 84

Query: 95  DACMASQLVTIKLLQRLKARYGDLYTEKNVAISGIHTHAGPGGYLQYIIYIVTSLGFVPQ 154
           DA MASQLVTIK+++RLK RYG+LYTE+NVAISG HTHAGPGGYLQYI+Y+VTSLGFV Q
Sbjct: 85  DAGMASQLVTIKVIERLKQRYGELYTEENVAISGTHTHAGPGGYLQYILYLVTSLGFVHQ 144

Query: 155 SFDVLVNGIENSIIQAHENLRPGSIFINKGKLLDAGVNRSPSAYLNNPAEERGKYKYNVD 214
           SF+ LV+GIE SIIQAHENLRPGSI INKG+LLDAGVNRSPSAYLNNPA ER KY+Y+VD
Sbjct: 145 SFNALVDGIEQSIIQAHENLRPGSILINKGELLDAGVNRSPSAYLNNPAHERSKYEYDVD 204

Query: 215 KEMTLLKFVDLEWGPIGSFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWFEKKSGISS 274
           KEMTL+KFVD +WGP+                            AR MEDWFE+++G   
Sbjct: 205 KEMTLVKFVDDQWGPV----------------------------ARIMEDWFERENGCR- 264

Query: 275 VFDISVDDRIPRRVSSIIPDLFINHHELLELAASFQSPSGGSATRILSSSRRVRGALRNA 334
               SVD   PRRVSSII D +    +L+E+A+S  S  G + TR+ S +RRVR   R+A
Sbjct: 265 ----SVDVESPRRVSSIISDPY--DQDLMEMASSLLSTGGKTVTRMSSVARRVRSRFRHA 324

Query: 335 EKPQFVSAFCQSNCGDVSPNVLGAFCIDTGLSCDFNHSTCNGKNELCYGRGPGYPDEFES 394
           +KP+FVSAFCQ+NCGDVSPNVLGAFCIDTGL C+FN STC GKNE CYGRGPGYPDEFES
Sbjct: 325 DKPRFVSAFCQTNCGDVSPNVLGAFCIDTGLPCEFNQSTCGGKNEQCYGRGPGYPDEFES 384

Query: 395 TRIIGKRQFRKAVDLFSNASEQLQGKVDYRHSYVDFSKLEVAIE-CGGTTEVVQTCPAAM 454
           TRIIG+RQF+KA DLF+ ASE++QGKVDYRH+YVDFS+LEV I    G +EVV+TCPAAM
Sbjct: 385 TRIIGERQFKKAADLFTKASEEIQGKVDYRHAYVDFSQLEVTINGQNGGSEVVKTCPAAM 444

Query: 455 GYSFAAGTTDGPGAFDFEQGDDKGNPFWKHIRNFLKKPKKEQIDCQQPKPILLDTGEMTK 514
           G+ FAAGTTDGPGAFDF+QGDD+GNPFW+ +RN LK P +EQ+ CQ+PKPILLDTGEM +
Sbjct: 445 GFGFAAGTTDGPGAFDFKQGDDQGNPFWRLVRNLLKNPTEEQVRCQRPKPILLDTGEMKQ 504

Query: 515 PYYWAPSILPIQILRIGQLIILGVPGEFSTMAGRRLRDAVQTVLTDGDKS-EFSGDIHIV 574
           PY WAPSILP+QILRIGQL+IL VPGEF+TMAGRRLRDAV+TVL +G    EFS    +V
Sbjct: 505 PYDWAPSILPVQILRIGQLVILCVPGEFTTMAGRRLRDAVKTVLKEGSNGREFS----VV 564

Query: 575 IAGLTNSYSQYVTTFEEYQVQRYEGASTLYGPHTLSAYIQEFKKLARALVDGQAVGAGPQ 634
           IAGLTNSYSQY+ TFEEYQVQRYEGASTLYGPHTLS YIQEFKKLA  L+  Q    GPQ
Sbjct: 565 IAGLTNSYSQYIATFEEYQVQRYEGASTLYGPHTLSGYIQEFKKLANDLLSAQTTDPGPQ 624

Query: 635 PPDLLDKQISFLPSVVMDTTPHSVKFGDVKSDVPKNSTFKRG-DTVTVTFWSACPRNDLM 694
           PPDLL KQIS L  VV D TP    FGDV SDVP+ S F++G D V V F SA PRNDLM
Sbjct: 625 PPDLLHKQISLLTPVVADMTPIGTAFGDVTSDVPRLSKFRKGADIVRVQFRSANPRNDLM 684

Query: 695 TEGTYALVE-ILHDKKLWVPAYDDDDFCLRFKWSRPFKLSSQSHATIEWRIPKSATPGVY 754
           TEGT+ALVE  L  ++ WVP YDDDDFCLRFKWSRPFKLS+QS ATIEWRIP++A+PGVY
Sbjct: 685 TEGTFALVERWLEGRETWVPVYDDDDFCLRFKWSRPFKLSTQSTATIEWRIPETASPGVY 732

Query: 755 RITHFGAAKKLFGSISHFTGSSSAFGV 778
           RITHFG+AK    SI HF+GSSSAF V
Sbjct: 745 RITHFGSAKTPISSIHHFSGSSSAFVV 732