PI0000428 (gene) Melon (PI 482460) v1

Overview
NamePI0000428
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptionalpha/beta-Hydrolases superfamily protein
Locationchr09: 20971092 .. 20972202 (+)
RNA-Seq ExpressionPI0000428
SyntenyPI0000428
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTTCTTCGATTTCTGGTTGTGAAATTTCAAGCTCCAAACTTCCATGGAAGCACCGTCTATTGCTTCGACTCGGTACTATAATCACCGACGCTTGTTGTCGATCCGATTTCACCGTCAACCGTTGGCTCACTGGAATCCTCGACTGGAAAATCCCCTCTTCTACCAAACCAATCGACGGCGTCTCCTCTTTCGATCTCACCATCGACACTTCCCGCAATCTCTGGGTTCGAATCTTCAATCCCGTTATTGACGATGAAGATTCAAATTTTCAATCTCTGCCGGTGATTTTCTATTTCCACGGTGGCGGCTTCGCTTTGTCTTACGCTGATTCAGCTCTCTCCCACACTTCCGCTCACCGATTCGCCAAACAATTACCCGCCGTTGTAATCTCCGTCAACTACCGCCTCGCTCCGGAGTTCCGGTACCCATGCCAATACGACGATGGATTCGACGCTTTGAAATTCATCGACGAAGTGGAGGAGGAAATCCTTCCGGCAAAGGCGGATTTAAGCCGTTGCTTCATTTTGGGGGAAAGCGCCGGAGGTAATCTTGGGCACCACGTGGCAGTGAGAGCGAGCGAGTACGCGTTAAAGAAAGTGAAATTGGTTGGGTTCATAGCGTCGCAGCCTTTTTTCGGCGGAGAGGAGAGAACAGAATCGGAGATTCGGTTGAGTAATCAGCGGCCGTTGAGTCTGAGGCTTTCGGATTGGTTTTGGAAGGCGTTTTTGCCGGAGGGGGAGGATCGGGATCACGTGGCGGCGAATGTGTTTGGACCGAAGGGAAGGGATGTTACGGAGGTGATGAAGTTTCCGGCGACGTTGGTTTTGGTGGGAGAATTGGATCTGTTGCAAGATGGACAGAGGAGGTATTACGAAGTGTTGAAGAGAATGGGGAAAGAAGTGAAAATGGTGGAATTTAAGAACGCCATTCATGGATTCTTTGCGTTTTGGGATCTGCCTCAGTATTCTTCCATGATTAAAGAGATAAAGGATTTTATTGCCACACAGTTTCAAAACTCCATTGTTTTTGCTTCTTCCTAACAAAGAAATAGGCTTTGCGGTTAACATTATAAATTATAATAATAACAATCATGTTTGGTTTTGAGAA

mRNA sequence

ATGTCTTCTTCGATTTCTGGTTGTGAAATTTCAAGCTCCAAACTTCCATGGAAGCACCGTCTATTGCTTCGACTCGGTACTATAATCACCGACGCTTGTTGTCGATCCGATTTCACCGTCAACCGTTGGCTCACTGGAATCCTCGACTGGAAAATCCCCTCTTCTACCAAACCAATCGACGGCGTCTCCTCTTTCGATCTCACCATCGACACTTCCCGCAATCTCTGGGTTCGAATCTTCAATCCCGTTATTGACGATGAAGATTCAAATTTTCAATCTCTGCCGGTGATTTTCTATTTCCACGGTGGCGGCTTCGCTTTGTCTTACGCTGATTCAGCTCTCTCCCACACTTCCGCTCACCGATTCGCCAAACAATTACCCGCCGTTGTAATCTCCGTCAACTACCGCCTCGCTCCGGAGTTCCGGTACCCATGCCAATACGACGATGGATTCGACGCTTTGAAATTCATCGACGAAGTGGAGGAGGAAATCCTTCCGGCAAAGGCGGATTTAAGCCGTTGCTTCATTTTGGGGGAAAGCGCCGGAGGTAATCTTGGGCACCACGTGGCAGTGAGAGCGAGCGAGTACGCGTTAAAGAAAGTGAAATTGGTTGGGTTCATAGCGTCGCAGCCTTTTTTCGGCGGAGAGGAGAGAACAGAATCGGAGATTCGGTTGAGTAATCAGCGGCCGTTGAGTCTGAGGCTTTCGGATTGGTTTTGGAAGGCGTTTTTGCCGGAGGGGGAGGATCGGGATCACGTGGCGGCGAATGTGTTTGGACCGAAGGGAAGGGATGTTACGGAGGTGATGAAGTTTCCGGCGACGTTGGTTTTGGTGGGAGAATTGGATCTGTTGCAAGATGGACAGAGGAGGTATTACGAAGTGTTGAAGAGAATGGGGAAAGAAGTGAAAATGGTGGAATTTAAGAACGCCATTCATGGATTCTTTGCGTTTTGGGATCTGCCTCAGTATTCTTCCATGATTAAAGAGATAAAGGATTTTATTGCCACACAGTTTCAAAACTCCATTGTTTTTGCTTCTTCCTAACAAAGAAATAGGCTTTGCGGTTAACATTATAAATTATAATAATAACAATCATGTTTGGTTTTGAGAA

Coding sequence (CDS)

ATGTCTTCTTCGATTTCTGGTTGTGAAATTTCAAGCTCCAAACTTCCATGGAAGCACCGTCTATTGCTTCGACTCGGTACTATAATCACCGACGCTTGTTGTCGATCCGATTTCACCGTCAACCGTTGGCTCACTGGAATCCTCGACTGGAAAATCCCCTCTTCTACCAAACCAATCGACGGCGTCTCCTCTTTCGATCTCACCATCGACACTTCCCGCAATCTCTGGGTTCGAATCTTCAATCCCGTTATTGACGATGAAGATTCAAATTTTCAATCTCTGCCGGTGATTTTCTATTTCCACGGTGGCGGCTTCGCTTTGTCTTACGCTGATTCAGCTCTCTCCCACACTTCCGCTCACCGATTCGCCAAACAATTACCCGCCGTTGTAATCTCCGTCAACTACCGCCTCGCTCCGGAGTTCCGGTACCCATGCCAATACGACGATGGATTCGACGCTTTGAAATTCATCGACGAAGTGGAGGAGGAAATCCTTCCGGCAAAGGCGGATTTAAGCCGTTGCTTCATTTTGGGGGAAAGCGCCGGAGGTAATCTTGGGCACCACGTGGCAGTGAGAGCGAGCGAGTACGCGTTAAAGAAAGTGAAATTGGTTGGGTTCATAGCGTCGCAGCCTTTTTTCGGCGGAGAGGAGAGAACAGAATCGGAGATTCGGTTGAGTAATCAGCGGCCGTTGAGTCTGAGGCTTTCGGATTGGTTTTGGAAGGCGTTTTTGCCGGAGGGGGAGGATCGGGATCACGTGGCGGCGAATGTGTTTGGACCGAAGGGAAGGGATGTTACGGAGGTGATGAAGTTTCCGGCGACGTTGGTTTTGGTGGGAGAATTGGATCTGTTGCAAGATGGACAGAGGAGGTATTACGAAGTGTTGAAGAGAATGGGGAAAGAAGTGAAAATGGTGGAATTTAAGAACGCCATTCATGGATTCTTTGCGTTTTGGGATCTGCCTCAGTATTCTTCCATGATTAAAGAGATAAAGGATTTTATTGCCACACAGTTTCAAAACTCCATTGTTTTTGCTTCTTCCTAA

Protein sequence

MSSSISGCEISSSKLPWKHRLLLRLGTIITDACCRSDFTVNRWLTGILDWKIPSSTKPIDGVSSFDLTIDTSRNLWVRIFNPVIDDEDSNFQSLPVIFYFHGGGFALSYADSALSHTSAHRFAKQLPAVVISVNYRLAPEFRYPCQYDDGFDALKFIDEVEEEILPAKADLSRCFILGESAGGNLGHHVAVRASEYALKKVKLVGFIASQPFFGGEERTESEIRLSNQRPLSLRLSDWFWKAFLPEGEDRDHVAANVFGPKGRDVTEVMKFPATLVLVGELDLLQDGQRRYYEVLKRMGKEVKMVEFKNAIHGFFAFWDLPQYSSMIKEIKDFIATQFQNSIVFASS
Homology
BLAST of PI0000428 vs. ExPASy Swiss-Prot
Match: Q9LT10 (Probable carboxylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=CXE18 PE=1 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 1.4e-78
Identity = 150/323 (46.44%), Postives = 205/323 (63.47%), Query Frame = 0

Query: 15  LPWKHRLLLRLGTIITDACCRSDFTVNRWLTGILDWKIPSSTKPIDGVSSFDLTIDTSRN 74
           LP K R+ L + + +TD   R D T+NR    + D++ P + KP++ VS+ D  +D SR+
Sbjct: 13  LPLKTRIALTVISTMTDNAQRPDGTINRRFLRLFDFRAPPNPKPVNIVSTSDFVVDQSRD 72

Query: 75  LWVRIFNPVIDDEDSNFQSLPVIFYFHGGGFALSYADSALSHTSAHRFAKQLPAVVISVN 134
           LW R++ P +  +      +PV+ +FHGGGFA    ++        RFA++LPA VISVN
Sbjct: 73  LWFRLYTPHVSGD-----KIPVVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPAYVISVN 132

Query: 135 YRLAPEFRYPCQYDDGFDALKFIDEVEEEILPAKADLSRCFILGESAGGNLGHHVAV--- 194
           YRLAPE RYP QYDDGFDALK+I+E    ILPA ADLSRCF  G+SAGGN+ H+VA+   
Sbjct: 133 YRLAPEHRYPAQYDDGFDALKYIEENHGSILPANADLSRCFFAGDSAGGNIAHNVAIRIC 192

Query: 195 RASEYALKKVKLVGFIASQPFFGGEERTESEIRLSNQRPLSLRLSDWFWKAFLPEGEDRD 254
           R    +   VKL+G I+ QPFFGGEERTE+E +L     +S   +DW WKA    G +RD
Sbjct: 193 REPRSSFTAVKLIGLISIQPFFGGEERTEAEKQLVGAPLVSPDRTDWCWKAM---GLNRD 252

Query: 255 HVAANVFGPKGRDVTEVMKFPATLVLVGELDLLQDGQRRYYEVLKRMGKEVKMVEFKNAI 314
           H A NV GP   D++  + +P T+V+V   D L+D QR YYE LK  GK+  ++E+ N  
Sbjct: 253 HEAVNVGGPNAVDISG-LDYPETMVVVAGFDPLKDWQRSYYEWLKLCGKKATLIEYPNMF 312

Query: 315 HGFFAFWDLPQYSSMIKEIKDFI 335
           H F+ F +LP+   +I  IKDF+
Sbjct: 313 HAFYIFPELPEAGQLIMRIKDFV 326

BLAST of PI0000428 vs. ExPASy Swiss-Prot
Match: Q940G6 (Gibberellin receptor GID1C OS=Arabidopsis thaliana OX=3702 GN=GID1C PE=1 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 2.6e-64
Identity = 135/315 (42.86%), Postives = 191/315 (60.63%), Query Frame = 0

Query: 35  RSDFTVNRWLTGILDWKIPSSTKPIDGVSSFDLTIDTSRNLWVRIFNPVIDDEDSNFQSL 94
           R D T NR L   LD K+P++  P++GV SFD+ ID   NL  R++ P       +   L
Sbjct: 36  RPDGTFNRHLAEFLDRKVPANANPVNGVFSFDVIIDRQTNLLSRVYRPADAGTSPSITDL 95

Query: 95  ---------PVIFYFHGGGFALSYADSALSHTSAHRFAKQLPAVVISVNYRLAPEFRYPC 154
                    PVI +FHGG FA S A+SA+  T   R      AVV+SVNYR APE RYPC
Sbjct: 96  QNPVDGEIVPVIVFFHGGSFAHSSANSAIYDTLCRRLVGLCGAVVVSVNYRRAPENRYPC 155

Query: 155 QYDDGFDALKFIDEVEEEILPAKADLS-RCFILGESAGGNLGHHVAVRASEYALKKVKLV 214
            YDDG+  LK+++      L +K D   R F+ G+S+GGN+ H+VAVRA E    ++ ++
Sbjct: 156 AYDDGWAVLKWVN--SSSWLRSKKDSKVRIFLAGDSSGGNIVHNVAVRAVE---SRIDVL 215

Query: 215 GFIASQPFFGGEERTESEIRLSNQRPLSLRLSDWFWKAFLPEGEDRDHVAANVFGPKGRD 274
           G I   P FGG ERTESE RL  +  +++R  DW+W+AFLPEGEDR+H A + FGP+ + 
Sbjct: 216 GNILLNPMFGGTERTESEKRLDGKYFVTVRDRDWYWRAFLPEGEDREHPACSPFGPRSKS 275

Query: 275 VTEVMKFPATLVLVGELDLLQDGQRRYYEVLKRMGKEVKMVEFKNAIHGFFAFWDLPQYS 334
           + E + FP +LV+V  LDL+QD Q +Y E LK+ G+EVK++  + A  GF+   +   + 
Sbjct: 276 L-EGLSFPKSLVVVAGLDLIQDWQLKYAEGLKKAGQEVKLLYLEQATIGFYLLPNNNHFH 335

Query: 335 SMIKEIKDFIATQFQ 340
           +++ EI  F+  + Q
Sbjct: 336 TVMDEIAAFVNAECQ 344

BLAST of PI0000428 vs. ExPASy Swiss-Prot
Match: Q9MAA7 (Gibberellin receptor GID1A OS=Arabidopsis thaliana OX=3702 GN=GID1A PE=1 SV=1)

HSP 1 Score: 238.4 bits (607), Expect = 1.2e-61
Identity = 130/312 (41.67%), Postives = 185/312 (59.29%), Query Frame = 0

Query: 35  RSDFTVNRWLTGILDWKIPSSTKPIDGVSSFDLTIDTSRNLWVRIFNPVIDDEDS----- 94
           R D T NR L   LD K+ ++  P+DGV SFD+ ID   NL  R++ P   D++      
Sbjct: 36  RPDGTFNRHLAEYLDRKVTANANPVDGVFSFDVLIDRRINLLSRVYRPAYADQEQPPSIL 95

Query: 95  ------NFQSLPVIFYFHGGGFALSYADSALSHTSAHRFAKQLPAVVISVNYRLAPEFRY 154
                 +   +PVI +FHGG FA S A+SA+  T   R       VV+SVNYR APE  Y
Sbjct: 96  DLEKPVDGDIVPVILFFHGGSFAHSSANSAIYDTLCRRLVGLCKCVVVSVNYRRAPENPY 155

Query: 155 PCQYDDGFDALKFIDEVEEEILPAKADLS-RCFILGESAGGNLGHHVAVRASEYALKKVK 214
           PC YDDG+ AL +++      L +K D     F+ G+S+GGN+ H+VA+RA E     + 
Sbjct: 156 PCAYDDGWIALNWVN--SRSWLKSKKDSKVHIFLAGDSSGGNIAHNVALRAGE---SGID 215

Query: 215 LVGFIASQPFFGGEERTESEIRLSNQRPLSLRLSDWFWKAFLPEGEDRDHVAANVFGPKG 274
           ++G I   P FGG ERTESE  L  +  +++R  DW+WKAFLPEGEDR+H A N F P+G
Sbjct: 216 VLGNILLNPMFGGNERTESEKSLDGKYFVTVRDRDWYWKAFLPEGEDREHPACNPFSPRG 275

Query: 275 RDVTEVMKFPATLVLVGELDLLQDGQRRYYEVLKRMGKEVKMVEFKNAIHGFFAFWDLPQ 334
           + + E + FP +LV+V  LDL++D Q  Y E LK+ G+EVK++  + A  GF+   +   
Sbjct: 276 KSL-EGVSFPKSLVVVAGLDLIRDWQLAYAEGLKKAGQEVKLMHLEKATVGFYLLPNNNH 335

BLAST of PI0000428 vs. ExPASy Swiss-Prot
Match: Q6L545 (Gibberellin receptor GID1 OS=Oryza sativa subsp. japonica OX=39947 GN=GID1 PE=1 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 2.9e-60
Identity = 131/319 (41.07%), Postives = 187/319 (58.62%), Query Frame = 0

Query: 35  RSDFTVNRWLTGILDWKIPSSTKPIDGVSSFDLTIDTSRNLWVRIF-------------- 94
           R+D T  R L   LD ++P++ +P++GVSSFD  ID S  L VRI+              
Sbjct: 36  RADGTFERDLGEYLDRRVPANARPLEGVSSFDHIIDQSVGLEVRIYRAAAEGDAEEGAAA 95

Query: 95  --NPVID--DEDSNFQSLPVIFYFHGGGFALSYADSALSHTSAHRFAKQLPAVVISVNYR 154
              P+++   +    +  PVI +FHGG F  S A S +  +   RF K    VV+SVNYR
Sbjct: 96  VTRPILEFLTDAPAAEPFPVIIFFHGGSFVHSSASSTIYDSLCRRFVKLSKGVVVSVNYR 155

Query: 155 LAPEFRYPCQYDDGFDALKFIDEVEEEILPAKADL-SRCFILGESAGGNLGHHVAVRASE 214
            APE RYPC YDDG+ ALK++  + +  + +  D  +R F+ G+S+GGN+ HHVAVRA++
Sbjct: 156 RAPEHRYPCAYDDGWTALKWV--MSQPFMRSGGDAQARVFLSGDSSGGNIAHHVAVRAAD 215

Query: 215 YALKKVKLVGFIASQPFFGGEERTESEIRLSNQRPLSLRLSDWFWKAFLPEGEDRDHVAA 274
              + VK+ G I     FGG ERTESE RL  +  ++L+  DW+WKA+LPE  DRDH A 
Sbjct: 216 ---EGVKVCGNILLNAMFGGTERTESERRLDGKYFVTLQDRDWYWKAYLPEDADRDHPAC 275

Query: 275 NVFGPKGRDVTEVMKFPATLVLVGELDLLQDGQRRYYEVLKRMGKEVKMVEFKNAIHGFF 334
           N FGP GR +   + F  +L++V  LDL  D Q  Y + L+  G  VK+V+ +NA  GF+
Sbjct: 276 NPFGPNGRRLGG-LPFAKSLIIVSGLDLTCDRQLAYADALREDGHHVKVVQCENATVGFY 335

BLAST of PI0000428 vs. ExPASy Swiss-Prot
Match: Q9LYC1 (Gibberellin receptor GID1B OS=Arabidopsis thaliana OX=3702 GN=GID1B PE=1 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 1.2e-58
Identity = 123/312 (39.42%), Postives = 187/312 (59.94%), Query Frame = 0

Query: 35  RSDFTVNRWLTGILDWKIPSSTKPIDGVSSFDLTIDTSRNLWVRIFNPV----------- 94
           R D + NR L   LD K+P+++ P+DGV SFD  +D++ NL  RI+ P            
Sbjct: 36  RPDGSFNRDLAEFLDRKVPANSFPLDGVFSFD-HVDSTTNLLTRIYQPASLLHQTRHGTL 95

Query: 95  -IDDEDSNFQSLPVIFYFHGGGFALSYADSALSHTSAHRFAKQLPAVVISVNYRLAPEFR 154
            +    S  + +PV+ +FHGG F  S A+SA+  T   R       VV+SV+YR +PE R
Sbjct: 96  ELTKPLSTTEIVPVLIFFHGGSFTHSSANSAIYDTFCRRLVTICGVVVVSVDYRRSPEHR 155

Query: 155 YPCQYDDGFDALKFIDEVEEEILPAKADLSRCFILGESAGGNLGHHVAVRASEYALKKVK 214
           YPC YDDG++AL ++ +    +   K      ++ G+S+GGN+ H+VAVRA+    + VK
Sbjct: 156 YPCAYDDGWNALNWV-KSRVWLQSGKDSNVYVYLAGDSSGGNIAHNVAVRATN---EGVK 215

Query: 215 LVGFIASQPFFGGEERTESEIRLSNQRPLSLRLSDWFWKAFLPEGEDRDHVAANVFGPKG 274
           ++G I   P FGG+ERT+SE  L  +  ++++  DW+W+A+LPEGEDRDH A N FGP+G
Sbjct: 216 VLGNILLHPMFGGQERTQSEKTLDGKYFVTIQDRDWYWRAYLPEGEDRDHPACNPFGPRG 275

Query: 275 RDVTEVMKFPATLVLVGELDLLQDGQRRYYEVLKRMGKEVKMVEFKNAIHGFFAFWDLPQ 334
           + +  V  FP +LV+V  LDL+QD Q  Y + LK+ G EV ++  K A  GF+   +   
Sbjct: 276 QSLKGV-NFPKSLVVVAGLDLVQDWQLAYVDGLKKTGLEVNLLYLKQATIGFYFLPNNDH 335

BLAST of PI0000428 vs. ExPASy TrEMBL
Match: A0A1S3CAT6 (probable carboxylesterase 18 OS=Cucumis melo OX=3656 GN=LOC103498754 PE=4 SV=1)

HSP 1 Score: 672.9 bits (1735), Expect = 7.0e-190
Identity = 325/346 (93.93%), Postives = 333/346 (96.24%), Query Frame = 0

Query: 2   SSSISGCEISSSKLPWKHRLLLRLGTIITDACCRSDFTVNRWLTGILDWKIPSSTKPIDG 61
           SSSIS CEISSSKLPWKHRLLLR+GTIITDACCRSDFTVNRWL+G LDWKIP STKPIDG
Sbjct: 3   SSSISACEISSSKLPWKHRLLLRIGTIITDACCRSDFTVNRWLSGTLDWKIPPSTKPIDG 62

Query: 62  VSSFDLTIDTSRNLWVRIFNPVIDDEDSNFQSLPVIFYFHGGGFALSYADSALSHTSAHR 121
           VSSFDLTIDTSRNLWVRIFNP+IDDEDSN QSLP+IFYFHGGGFA SYADSALSHTSAHR
Sbjct: 63  VSSFDLTIDTSRNLWVRIFNPIIDDEDSNLQSLPLIFYFHGGGFAFSYADSALSHTSAHR 122

Query: 122 FAKQLPAVVISVNYRLAPEFRYPCQYDDGFDALKFIDEVEEEILPAKADLSRCFILGESA 181
           FAKQLPAVVISVNYRLAPEFRYPCQYDDGFDALKFIDEV EEILPAKADLSRCFILGESA
Sbjct: 123 FAKQLPAVVISVNYRLAPEFRYPCQYDDGFDALKFIDEVGEEILPAKADLSRCFILGESA 182

Query: 182 GGNLGHHVAVRASEYALKKVKLVGFIASQPFFGGEERTESEIRLSNQRPLSLRLSDWFWK 241
           GGNLGHHVAVRASEY LKKV+LVGFIASQPFFGGEERTESEIRL NQRPL+LRLSDWFWK
Sbjct: 183 GGNLGHHVAVRASEYTLKKVRLVGFIASQPFFGGEERTESEIRLRNQRPLNLRLSDWFWK 242

Query: 242 AFLPEGEDRDHVAANVFGPKGRDVTEVMKFPATLVLVGELDLLQDGQRRYYEVLKRMGKE 301
           AFLPEGEDRDH AANVFGPKGRDV EVMKFPATLVLVGELDLLQDGQRRYYE LKRMGKE
Sbjct: 243 AFLPEGEDRDHAAANVFGPKGRDVREVMKFPATLVLVGELDLLQDGQRRYYEGLKRMGKE 302

Query: 302 VKMVEFKNAIHGFFAFWDLPQYSSMIKEIKDFIATQFQNSIVFASS 348
           VKMVEF+NAIHGFFAFWDLPQYSSMIKE+KDFIAT  QNSIVFASS
Sbjct: 303 VKMVEFENAIHGFFAFWDLPQYSSMIKEMKDFIATHTQNSIVFASS 348

BLAST of PI0000428 vs. ExPASy TrEMBL
Match: A0A5D3BN57 (Putative carboxylesterase 18 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001350 PE=4 SV=1)

HSP 1 Score: 671.8 bits (1732), Expect = 1.6e-189
Identity = 325/346 (93.93%), Postives = 332/346 (95.95%), Query Frame = 0

Query: 2   SSSISGCEISSSKLPWKHRLLLRLGTIITDACCRSDFTVNRWLTGILDWKIPSSTKPIDG 61
           SSSIS CEISSSKLPWKHRLLLR+GTIITDACCRSDFTVNRWL+G LDWKIP STKPIDG
Sbjct: 3   SSSISACEISSSKLPWKHRLLLRIGTIITDACCRSDFTVNRWLSGTLDWKIPPSTKPIDG 62

Query: 62  VSSFDLTIDTSRNLWVRIFNPVIDDEDSNFQSLPVIFYFHGGGFALSYADSALSHTSAHR 121
           VSSFDLTIDTSRNLWVRIFNP IDDEDSN QSLP+IFYFHGGGFA SYADSALSHTSAHR
Sbjct: 63  VSSFDLTIDTSRNLWVRIFNPTIDDEDSNLQSLPLIFYFHGGGFAFSYADSALSHTSAHR 122

Query: 122 FAKQLPAVVISVNYRLAPEFRYPCQYDDGFDALKFIDEVEEEILPAKADLSRCFILGESA 181
           FAKQLPAVVISVNYRLAPEFRYPCQYDDGFDALKFIDEV EEILPAKADLSRCFILGESA
Sbjct: 123 FAKQLPAVVISVNYRLAPEFRYPCQYDDGFDALKFIDEVGEEILPAKADLSRCFILGESA 182

Query: 182 GGNLGHHVAVRASEYALKKVKLVGFIASQPFFGGEERTESEIRLSNQRPLSLRLSDWFWK 241
           GGNLGHHVAVRASEY LKKV+LVGFIASQPFFGGEERTESEIRL NQRPL+LRLSDWFWK
Sbjct: 183 GGNLGHHVAVRASEYTLKKVRLVGFIASQPFFGGEERTESEIRLRNQRPLNLRLSDWFWK 242

Query: 242 AFLPEGEDRDHVAANVFGPKGRDVTEVMKFPATLVLVGELDLLQDGQRRYYEVLKRMGKE 301
           AFLPEGEDRDH AANVFGPKGRDV EVMKFPATLVLVGELDLLQDGQRRYYE LKRMGKE
Sbjct: 243 AFLPEGEDRDHAAANVFGPKGRDVREVMKFPATLVLVGELDLLQDGQRRYYEGLKRMGKE 302

Query: 302 VKMVEFKNAIHGFFAFWDLPQYSSMIKEIKDFIATQFQNSIVFASS 348
           VKMVEF+NAIHGFFAFWDLPQYSSMIKE+KDFIAT  QNSIVFASS
Sbjct: 303 VKMVEFENAIHGFFAFWDLPQYSSMIKEMKDFIATHTQNSIVFASS 348

BLAST of PI0000428 vs. ExPASy TrEMBL
Match: A0A0A0KUP3 (Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G647370 PE=4 SV=1)

HSP 1 Score: 662.5 bits (1708), Expect = 9.5e-187
Identity = 321/347 (92.51%), Postives = 331/347 (95.39%), Query Frame = 0

Query: 1   MSSSISGCEISSSKLPWKHRLLLRLGTIITDACCRSDFTVNRWLTGILDWKIPSSTKPID 60
           MSSS S  EISSSKLPWKHRLLLR+G+ ITDACCRSDFTVNRW TGILDWKIP STKPID
Sbjct: 1   MSSSTSASEISSSKLPWKHRLLLRIGSTITDACCRSDFTVNRWFTGILDWKIPPSTKPID 60

Query: 61  GVSSFDLTIDTSRNLWVRIFNPVIDDEDSNFQSLPVIFYFHGGGFALSYADSALSHTSAH 120
           GVSSFDLTIDTSRNLWVRIFNPVID EDS+ QSLP+IFYFHGGGFA SYADSALSHTSAH
Sbjct: 61  GVSSFDLTIDTSRNLWVRIFNPVIDGEDSDIQSLPLIFYFHGGGFAFSYADSALSHTSAH 120

Query: 121 RFAKQLPAVVISVNYRLAPEFRYPCQYDDGFDALKFIDEVEEEILPAKADLSRCFILGES 180
           RFAKQLPAVVISVNYRLAPEFRYPCQYDDGFDALKFIDEV EEILPAKADL+RCFILGES
Sbjct: 121 RFAKQLPAVVISVNYRLAPEFRYPCQYDDGFDALKFIDEVGEEILPAKADLTRCFILGES 180

Query: 181 AGGNLGHHVAVRASEYALKKVKLVGFIASQPFFGGEERTESEIRLSNQRPLSLRLSDWFW 240
           AGGNLGHHVAVRASEY LKKVK+VGFIASQPFFGGEERTESEIRLSNQRPLSLRLSDWFW
Sbjct: 181 AGGNLGHHVAVRASEYTLKKVKMVGFIASQPFFGGEERTESEIRLSNQRPLSLRLSDWFW 240

Query: 241 KAFLPEGEDRDHVAANVFGPKGRDVTEVMKFPATLVLVGELDLLQDGQRRYYEVLKRMGK 300
           KAFLPEGEDRDH AANVFGPKGRDVTEVMKFPATLV+VGELDLLQDGQRRYYE LKRMGK
Sbjct: 241 KAFLPEGEDRDHGAANVFGPKGRDVTEVMKFPATLVMVGELDLLQDGQRRYYEGLKRMGK 300

Query: 301 EVKMVEFKNAIHGFFAFWDLPQYSSMIKEIKDFIATQFQNSIVFASS 348
           EVKMVEF+NAIHGFFAFWDLPQYSSM+KE+KDFIAT  QNSIV ASS
Sbjct: 301 EVKMVEFENAIHGFFAFWDLPQYSSMMKEMKDFIATHIQNSIVSASS 347

BLAST of PI0000428 vs. ExPASy TrEMBL
Match: A0A6J1EWT7 (probable carboxylesterase 18 OS=Cucurbita moschata OX=3662 GN=LOC111438887 PE=4 SV=1)

HSP 1 Score: 533.1 bits (1372), Expect = 8.7e-148
Identity = 256/330 (77.58%), Postives = 284/330 (86.06%), Query Frame = 0

Query: 11  SSSKLPWKHRLLLRLGTIITDACCRSDFTVNRWLTGILDWKIPSSTKPIDGVSSFDLTID 70
           S+ +LPWKHRL+LRLGTI T+  CRSDFT+NRWLTGILD + P S+ PIDGVSS DL ID
Sbjct: 10  STLRLPWKHRLMLRLGTIATNTFCRSDFTINRWLTGILDRRTPPSSIPIDGVSSSDLLID 69

Query: 71  TSRNLWVRIFNPVIDDEDSNFQSLPVIFYFHGGGFALSYADSALSHTSAHRFAKQLPAVV 130
            SRNLWVRIF P+        QSLPVIFY+HGGGFA SYADS+L HT+AH+FAKQL AVV
Sbjct: 70  PSRNLWVRIFTPI-----DAVQSLPVIFYYHGGGFAFSYADSSLYHTTAHQFAKQLRAVV 129

Query: 131 ISVNYRLAPEFRYPCQYDDGFDALKFIDEVEEEILPAKADLSRCFILGESAGGNLGHHVA 190
           ISVNYRLAPEFR+PCQYDDGFDALKFIDE+++  LPA ADL R FILGESAGGNLGHHVA
Sbjct: 130 ISVNYRLAPEFRFPCQYDDGFDALKFIDEIDDGTLPASADLGRSFILGESAGGNLGHHVA 189

Query: 191 VRASEYALKKVKLVGFIASQPFFGGEERTESEIRLSNQRPLSLRLSDWFWKAFLPEGEDR 250
           VRASEYA KK+KL+GFIA+QPFFG EERTESEIRL NQ PLSLRLSDWFWKAFLP G DR
Sbjct: 190 VRASEYAFKKLKLIGFIATQPFFGAEERTESEIRLCNQAPLSLRLSDWFWKAFLPVGSDR 249

Query: 251 DHVAANVFGPKGRDVTEVMKFPATLVLVGELDLLQDGQRRYYEVLKRMGKEVKMVEFKNA 310
           DH AANVFGP GRD++ V +FPAT+VLVG  DLLQDGQRRYYE LKRMGKEVKMVE  NA
Sbjct: 250 DHAAANVFGPNGRDISGVERFPATVVLVGGFDLLQDGQRRYYEGLKRMGKEVKMVELPNA 309

Query: 311 IHGFFAFWDLPQYSSMIKEIKDFIATQFQN 341
           IHGFF F DLP+YSSMI+E++DFIA Q+QN
Sbjct: 310 IHGFFCFPDLPEYSSMIQEVRDFIAAQWQN 334

BLAST of PI0000428 vs. ExPASy TrEMBL
Match: A0A6J1IB87 (probable carboxylesterase 18 OS=Cucurbita maxima OX=3661 GN=LOC111471943 PE=4 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 2.5e-147
Identity = 255/330 (77.27%), Postives = 284/330 (86.06%), Query Frame = 0

Query: 11  SSSKLPWKHRLLLRLGTIITDACCRSDFTVNRWLTGILDWKIPSSTKPIDGVSSFDLTID 70
           S+ +LPWKHRL+LRLGTI T+  CRSDFT+NRWLTGILD + P S+KPIDGVSS DL ID
Sbjct: 10  STLRLPWKHRLMLRLGTIATNTFCRSDFTINRWLTGILDRRTPPSSKPIDGVSSSDLLID 69

Query: 71  TSRNLWVRIFNPVIDDEDSNFQSLPVIFYFHGGGFALSYADSALSHTSAHRFAKQLPAVV 130
            SRNLWVRIF P+ D      QSLPVIFY+HGGGFA SYADS+L HT+AH+FAKQL AVV
Sbjct: 70  KSRNLWVRIFTPIDD-----VQSLPVIFYYHGGGFAFSYADSSLYHTTAHQFAKQLRAVV 129

Query: 131 ISVNYRLAPEFRYPCQYDDGFDALKFIDEVEEEILPAKADLSRCFILGESAGGNLGHHVA 190
           ISVNYRLAPEFR+PCQYDDGFDALKFIDE+++  LPA ADL R FILGESAGGNLGHHVA
Sbjct: 130 ISVNYRLAPEFRFPCQYDDGFDALKFIDEIDDGTLPASADLGRSFILGESAGGNLGHHVA 189

Query: 191 VRASEYALKKVKLVGFIASQPFFGGEERTESEIRLSNQRPLSLRLSDWFWKAFLPEGEDR 250
           VRASEYA KK+KL+GFIA+QPF G EERTESEIRL NQ PLSL+L+DWFWKAFLP G DR
Sbjct: 190 VRASEYAFKKLKLIGFIATQPFIGAEERTESEIRLCNQPPLSLKLTDWFWKAFLPVGSDR 249

Query: 251 DHVAANVFGPKGRDVTEVMKFPATLVLVGELDLLQDGQRRYYEVLKRMGKEVKMVEFKNA 310
           DH AANVFGP GRD++ V KFPAT+VLVG  DLLQDGQRRYYE LKRMGKEVKMVE  NA
Sbjct: 250 DHAAANVFGPNGRDISGVEKFPATVVLVGGFDLLQDGQRRYYEGLKRMGKEVKMVELPNA 309

Query: 311 IHGFFAFWDLPQYSSMIKEIKDFIATQFQN 341
           IHGFF F DLP+YSSMI+ ++DFIA Q+QN
Sbjct: 310 IHGFFCFPDLPEYSSMIQAVRDFIAAQWQN 334

BLAST of PI0000428 vs. NCBI nr
Match: XP_008459712.1 (PREDICTED: probable carboxylesterase 18 [Cucumis melo])

HSP 1 Score: 672.9 bits (1735), Expect = 1.4e-189
Identity = 325/346 (93.93%), Postives = 333/346 (96.24%), Query Frame = 0

Query: 2   SSSISGCEISSSKLPWKHRLLLRLGTIITDACCRSDFTVNRWLTGILDWKIPSSTKPIDG 61
           SSSIS CEISSSKLPWKHRLLLR+GTIITDACCRSDFTVNRWL+G LDWKIP STKPIDG
Sbjct: 3   SSSISACEISSSKLPWKHRLLLRIGTIITDACCRSDFTVNRWLSGTLDWKIPPSTKPIDG 62

Query: 62  VSSFDLTIDTSRNLWVRIFNPVIDDEDSNFQSLPVIFYFHGGGFALSYADSALSHTSAHR 121
           VSSFDLTIDTSRNLWVRIFNP+IDDEDSN QSLP+IFYFHGGGFA SYADSALSHTSAHR
Sbjct: 63  VSSFDLTIDTSRNLWVRIFNPIIDDEDSNLQSLPLIFYFHGGGFAFSYADSALSHTSAHR 122

Query: 122 FAKQLPAVVISVNYRLAPEFRYPCQYDDGFDALKFIDEVEEEILPAKADLSRCFILGESA 181
           FAKQLPAVVISVNYRLAPEFRYPCQYDDGFDALKFIDEV EEILPAKADLSRCFILGESA
Sbjct: 123 FAKQLPAVVISVNYRLAPEFRYPCQYDDGFDALKFIDEVGEEILPAKADLSRCFILGESA 182

Query: 182 GGNLGHHVAVRASEYALKKVKLVGFIASQPFFGGEERTESEIRLSNQRPLSLRLSDWFWK 241
           GGNLGHHVAVRASEY LKKV+LVGFIASQPFFGGEERTESEIRL NQRPL+LRLSDWFWK
Sbjct: 183 GGNLGHHVAVRASEYTLKKVRLVGFIASQPFFGGEERTESEIRLRNQRPLNLRLSDWFWK 242

Query: 242 AFLPEGEDRDHVAANVFGPKGRDVTEVMKFPATLVLVGELDLLQDGQRRYYEVLKRMGKE 301
           AFLPEGEDRDH AANVFGPKGRDV EVMKFPATLVLVGELDLLQDGQRRYYE LKRMGKE
Sbjct: 243 AFLPEGEDRDHAAANVFGPKGRDVREVMKFPATLVLVGELDLLQDGQRRYYEGLKRMGKE 302

Query: 302 VKMVEFKNAIHGFFAFWDLPQYSSMIKEIKDFIATQFQNSIVFASS 348
           VKMVEF+NAIHGFFAFWDLPQYSSMIKE+KDFIAT  QNSIVFASS
Sbjct: 303 VKMVEFENAIHGFFAFWDLPQYSSMIKEMKDFIATHTQNSIVFASS 348

BLAST of PI0000428 vs. NCBI nr
Match: KAA0039342.1 (putative carboxylesterase 18 [Cucumis melo var. makuwa] >TYK00525.1 putative carboxylesterase 18 [Cucumis melo var. makuwa])

HSP 1 Score: 671.8 bits (1732), Expect = 3.2e-189
Identity = 325/346 (93.93%), Postives = 332/346 (95.95%), Query Frame = 0

Query: 2   SSSISGCEISSSKLPWKHRLLLRLGTIITDACCRSDFTVNRWLTGILDWKIPSSTKPIDG 61
           SSSIS CEISSSKLPWKHRLLLR+GTIITDACCRSDFTVNRWL+G LDWKIP STKPIDG
Sbjct: 3   SSSISACEISSSKLPWKHRLLLRIGTIITDACCRSDFTVNRWLSGTLDWKIPPSTKPIDG 62

Query: 62  VSSFDLTIDTSRNLWVRIFNPVIDDEDSNFQSLPVIFYFHGGGFALSYADSALSHTSAHR 121
           VSSFDLTIDTSRNLWVRIFNP IDDEDSN QSLP+IFYFHGGGFA SYADSALSHTSAHR
Sbjct: 63  VSSFDLTIDTSRNLWVRIFNPTIDDEDSNLQSLPLIFYFHGGGFAFSYADSALSHTSAHR 122

Query: 122 FAKQLPAVVISVNYRLAPEFRYPCQYDDGFDALKFIDEVEEEILPAKADLSRCFILGESA 181
           FAKQLPAVVISVNYRLAPEFRYPCQYDDGFDALKFIDEV EEILPAKADLSRCFILGESA
Sbjct: 123 FAKQLPAVVISVNYRLAPEFRYPCQYDDGFDALKFIDEVGEEILPAKADLSRCFILGESA 182

Query: 182 GGNLGHHVAVRASEYALKKVKLVGFIASQPFFGGEERTESEIRLSNQRPLSLRLSDWFWK 241
           GGNLGHHVAVRASEY LKKV+LVGFIASQPFFGGEERTESEIRL NQRPL+LRLSDWFWK
Sbjct: 183 GGNLGHHVAVRASEYTLKKVRLVGFIASQPFFGGEERTESEIRLRNQRPLNLRLSDWFWK 242

Query: 242 AFLPEGEDRDHVAANVFGPKGRDVTEVMKFPATLVLVGELDLLQDGQRRYYEVLKRMGKE 301
           AFLPEGEDRDH AANVFGPKGRDV EVMKFPATLVLVGELDLLQDGQRRYYE LKRMGKE
Sbjct: 243 AFLPEGEDRDHAAANVFGPKGRDVREVMKFPATLVLVGELDLLQDGQRRYYEGLKRMGKE 302

Query: 302 VKMVEFKNAIHGFFAFWDLPQYSSMIKEIKDFIATQFQNSIVFASS 348
           VKMVEF+NAIHGFFAFWDLPQYSSMIKE+KDFIAT  QNSIVFASS
Sbjct: 303 VKMVEFENAIHGFFAFWDLPQYSSMIKEMKDFIATHTQNSIVFASS 348

BLAST of PI0000428 vs. NCBI nr
Match: XP_004141616.1 (probable carboxylesterase 18 [Cucumis sativus] >KGN52614.1 hypothetical protein Csa_009173 [Cucumis sativus])

HSP 1 Score: 662.5 bits (1708), Expect = 2.0e-186
Identity = 321/347 (92.51%), Postives = 331/347 (95.39%), Query Frame = 0

Query: 1   MSSSISGCEISSSKLPWKHRLLLRLGTIITDACCRSDFTVNRWLTGILDWKIPSSTKPID 60
           MSSS S  EISSSKLPWKHRLLLR+G+ ITDACCRSDFTVNRW TGILDWKIP STKPID
Sbjct: 1   MSSSTSASEISSSKLPWKHRLLLRIGSTITDACCRSDFTVNRWFTGILDWKIPPSTKPID 60

Query: 61  GVSSFDLTIDTSRNLWVRIFNPVIDDEDSNFQSLPVIFYFHGGGFALSYADSALSHTSAH 120
           GVSSFDLTIDTSRNLWVRIFNPVID EDS+ QSLP+IFYFHGGGFA SYADSALSHTSAH
Sbjct: 61  GVSSFDLTIDTSRNLWVRIFNPVIDGEDSDIQSLPLIFYFHGGGFAFSYADSALSHTSAH 120

Query: 121 RFAKQLPAVVISVNYRLAPEFRYPCQYDDGFDALKFIDEVEEEILPAKADLSRCFILGES 180
           RFAKQLPAVVISVNYRLAPEFRYPCQYDDGFDALKFIDEV EEILPAKADL+RCFILGES
Sbjct: 121 RFAKQLPAVVISVNYRLAPEFRYPCQYDDGFDALKFIDEVGEEILPAKADLTRCFILGES 180

Query: 181 AGGNLGHHVAVRASEYALKKVKLVGFIASQPFFGGEERTESEIRLSNQRPLSLRLSDWFW 240
           AGGNLGHHVAVRASEY LKKVK+VGFIASQPFFGGEERTESEIRLSNQRPLSLRLSDWFW
Sbjct: 181 AGGNLGHHVAVRASEYTLKKVKMVGFIASQPFFGGEERTESEIRLSNQRPLSLRLSDWFW 240

Query: 241 KAFLPEGEDRDHVAANVFGPKGRDVTEVMKFPATLVLVGELDLLQDGQRRYYEVLKRMGK 300
           KAFLPEGEDRDH AANVFGPKGRDVTEVMKFPATLV+VGELDLLQDGQRRYYE LKRMGK
Sbjct: 241 KAFLPEGEDRDHGAANVFGPKGRDVTEVMKFPATLVMVGELDLLQDGQRRYYEGLKRMGK 300

Query: 301 EVKMVEFKNAIHGFFAFWDLPQYSSMIKEIKDFIATQFQNSIVFASS 348
           EVKMVEF+NAIHGFFAFWDLPQYSSM+KE+KDFIAT  QNSIV ASS
Sbjct: 301 EVKMVEFENAIHGFFAFWDLPQYSSMMKEMKDFIATHIQNSIVSASS 347

BLAST of PI0000428 vs. NCBI nr
Match: XP_038890362.1 (probable carboxylesterase 18 [Benincasa hispida])

HSP 1 Score: 619.8 bits (1597), Expect = 1.5e-173
Identity = 300/350 (85.71%), Postives = 317/350 (90.57%), Query Frame = 0

Query: 1   MSSSISGCEISSSKLPWKHRLLLRLGTIITDACCRSDFTVNRWLTGILDWKIPSSTKPID 60
           MSSSIS CEISSSKLPWK+RLLLRLGTIITDACCRS+FTVNRWLTGILDWKIP STKPID
Sbjct: 1   MSSSISACEISSSKLPWKYRLLLRLGTIITDACCRSNFTVNRWLTGILDWKIPPSTKPID 60

Query: 61  GVSSFDLTIDTSRNLWVRIFNPVIDDEDSNFQSLPVIFYFHGGGFALSYADSALSHTSAH 120
           GVSSFDLTIDTSRNLWVRIFNP+ID E  N QS+PVIFYFHGGGF  SYADSAL H +AH
Sbjct: 61  GVSSFDLTIDTSRNLWVRIFNPIIDGEHPNLQSVPVIFYFHGGGFVFSYADSALFHMAAH 120

Query: 121 RFAKQLPAVVISVNYRLAPEFRYPCQYDDGFDALKFIDEVEEEILPAKADLSRCFILGES 180
           R AKQL AVVISVNYRLAPEFR PCQYDDGFDA+KFIDE +E ILP KADLSRCFILGES
Sbjct: 121 RLAKQLHAVVISVNYRLAPEFRCPCQYDDGFDAVKFIDETDEGILPEKADLSRCFILGES 180

Query: 181 AGGNLGHHVAVRASEYALKKVKLVGFIASQPFFGGEERTESEIRLSNQRPLSLRLSDWFW 240
           AGGNLGHHVAVRASEY  KK+KL+GFIASQPFFGGEERTESE+RL NQ PLSLRL DWFW
Sbjct: 181 AGGNLGHHVAVRASEYTFKKLKLIGFIASQPFFGGEERTESEMRLRNQPPLSLRLCDWFW 240

Query: 241 KAFLPEGEDRDHVAANVFGPKGRDVTEVMKFPATLVLVGELDLLQDGQRRYYEVLKRMGK 300
           KAFLPEGE+RDH AANVFGP GRDV+EV+KFPAT+V VGELDLLQDGQR+YYE LKRMGK
Sbjct: 241 KAFLPEGENRDHAAANVFGPNGRDVSEVVKFPATVVFVGELDLLQDGQRKYYEGLKRMGK 300

Query: 301 EVKMVEFKNAIHGFFAFWDLPQYSSMIKEIKDFIATQFQNS---IVFASS 348
            VKMVEF NAIHGFFAFWDLPQYSSMIKE+KDFIAT+F NS   IV  SS
Sbjct: 301 NVKMVEFPNAIHGFFAFWDLPQYSSMIKEMKDFIATEFHNSPPLIVHGSS 350

BLAST of PI0000428 vs. NCBI nr
Match: XP_022932482.1 (probable carboxylesterase 18 [Cucurbita moschata])

HSP 1 Score: 533.1 bits (1372), Expect = 1.8e-147
Identity = 256/330 (77.58%), Postives = 284/330 (86.06%), Query Frame = 0

Query: 11  SSSKLPWKHRLLLRLGTIITDACCRSDFTVNRWLTGILDWKIPSSTKPIDGVSSFDLTID 70
           S+ +LPWKHRL+LRLGTI T+  CRSDFT+NRWLTGILD + P S+ PIDGVSS DL ID
Sbjct: 10  STLRLPWKHRLMLRLGTIATNTFCRSDFTINRWLTGILDRRTPPSSIPIDGVSSSDLLID 69

Query: 71  TSRNLWVRIFNPVIDDEDSNFQSLPVIFYFHGGGFALSYADSALSHTSAHRFAKQLPAVV 130
            SRNLWVRIF P+        QSLPVIFY+HGGGFA SYADS+L HT+AH+FAKQL AVV
Sbjct: 70  PSRNLWVRIFTPI-----DAVQSLPVIFYYHGGGFAFSYADSSLYHTTAHQFAKQLRAVV 129

Query: 131 ISVNYRLAPEFRYPCQYDDGFDALKFIDEVEEEILPAKADLSRCFILGESAGGNLGHHVA 190
           ISVNYRLAPEFR+PCQYDDGFDALKFIDE+++  LPA ADL R FILGESAGGNLGHHVA
Sbjct: 130 ISVNYRLAPEFRFPCQYDDGFDALKFIDEIDDGTLPASADLGRSFILGESAGGNLGHHVA 189

Query: 191 VRASEYALKKVKLVGFIASQPFFGGEERTESEIRLSNQRPLSLRLSDWFWKAFLPEGEDR 250
           VRASEYA KK+KL+GFIA+QPFFG EERTESEIRL NQ PLSLRLSDWFWKAFLP G DR
Sbjct: 190 VRASEYAFKKLKLIGFIATQPFFGAEERTESEIRLCNQAPLSLRLSDWFWKAFLPVGSDR 249

Query: 251 DHVAANVFGPKGRDVTEVMKFPATLVLVGELDLLQDGQRRYYEVLKRMGKEVKMVEFKNA 310
           DH AANVFGP GRD++ V +FPAT+VLVG  DLLQDGQRRYYE LKRMGKEVKMVE  NA
Sbjct: 250 DHAAANVFGPNGRDISGVERFPATVVLVGGFDLLQDGQRRYYEGLKRMGKEVKMVELPNA 309

Query: 311 IHGFFAFWDLPQYSSMIKEIKDFIATQFQN 341
           IHGFF F DLP+YSSMI+E++DFIA Q+QN
Sbjct: 310 IHGFFCFPDLPEYSSMIQEVRDFIAAQWQN 334

BLAST of PI0000428 vs. TAIR 10
Match: AT5G23530.1 (carboxyesterase 18 )

HSP 1 Score: 294.7 bits (753), Expect = 1.0e-79
Identity = 150/323 (46.44%), Postives = 205/323 (63.47%), Query Frame = 0

Query: 15  LPWKHRLLLRLGTIITDACCRSDFTVNRWLTGILDWKIPSSTKPIDGVSSFDLTIDTSRN 74
           LP K R+ L + + +TD   R D T+NR    + D++ P + KP++ VS+ D  +D SR+
Sbjct: 13  LPLKTRIALTVISTMTDNAQRPDGTINRRFLRLFDFRAPPNPKPVNIVSTSDFVVDQSRD 72

Query: 75  LWVRIFNPVIDDEDSNFQSLPVIFYFHGGGFALSYADSALSHTSAHRFAKQLPAVVISVN 134
           LW R++ P +  +      +PV+ +FHGGGFA    ++        RFA++LPA VISVN
Sbjct: 73  LWFRLYTPHVSGD-----KIPVVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPAYVISVN 132

Query: 135 YRLAPEFRYPCQYDDGFDALKFIDEVEEEILPAKADLSRCFILGESAGGNLGHHVAV--- 194
           YRLAPE RYP QYDDGFDALK+I+E    ILPA ADLSRCF  G+SAGGN+ H+VA+   
Sbjct: 133 YRLAPEHRYPAQYDDGFDALKYIEENHGSILPANADLSRCFFAGDSAGGNIAHNVAIRIC 192

Query: 195 RASEYALKKVKLVGFIASQPFFGGEERTESEIRLSNQRPLSLRLSDWFWKAFLPEGEDRD 254
           R    +   VKL+G I+ QPFFGGEERTE+E +L     +S   +DW WKA    G +RD
Sbjct: 193 REPRSSFTAVKLIGLISIQPFFGGEERTEAEKQLVGAPLVSPDRTDWCWKAM---GLNRD 252

Query: 255 HVAANVFGPKGRDVTEVMKFPATLVLVGELDLLQDGQRRYYEVLKRMGKEVKMVEFKNAI 314
           H A NV GP   D++  + +P T+V+V   D L+D QR YYE LK  GK+  ++E+ N  
Sbjct: 253 HEAVNVGGPNAVDISG-LDYPETMVVVAGFDPLKDWQRSYYEWLKLCGKKATLIEYPNMF 312

Query: 315 HGFFAFWDLPQYSSMIKEIKDFI 335
           H F+ F +LP+   +I  IKDF+
Sbjct: 313 HAFYIFPELPEAGQLIMRIKDFV 326

BLAST of PI0000428 vs. TAIR 10
Match: AT5G27320.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 247.3 bits (630), Expect = 1.8e-65
Identity = 135/315 (42.86%), Postives = 191/315 (60.63%), Query Frame = 0

Query: 35  RSDFTVNRWLTGILDWKIPSSTKPIDGVSSFDLTIDTSRNLWVRIFNPVIDDEDSNFQSL 94
           R D T NR L   LD K+P++  P++GV SFD+ ID   NL  R++ P       +   L
Sbjct: 36  RPDGTFNRHLAEFLDRKVPANANPVNGVFSFDVIIDRQTNLLSRVYRPADAGTSPSITDL 95

Query: 95  ---------PVIFYFHGGGFALSYADSALSHTSAHRFAKQLPAVVISVNYRLAPEFRYPC 154
                    PVI +FHGG FA S A+SA+  T   R      AVV+SVNYR APE RYPC
Sbjct: 96  QNPVDGEIVPVIVFFHGGSFAHSSANSAIYDTLCRRLVGLCGAVVVSVNYRRAPENRYPC 155

Query: 155 QYDDGFDALKFIDEVEEEILPAKADLS-RCFILGESAGGNLGHHVAVRASEYALKKVKLV 214
            YDDG+  LK+++      L +K D   R F+ G+S+GGN+ H+VAVRA E    ++ ++
Sbjct: 156 AYDDGWAVLKWVN--SSSWLRSKKDSKVRIFLAGDSSGGNIVHNVAVRAVE---SRIDVL 215

Query: 215 GFIASQPFFGGEERTESEIRLSNQRPLSLRLSDWFWKAFLPEGEDRDHVAANVFGPKGRD 274
           G I   P FGG ERTESE RL  +  +++R  DW+W+AFLPEGEDR+H A + FGP+ + 
Sbjct: 216 GNILLNPMFGGTERTESEKRLDGKYFVTVRDRDWYWRAFLPEGEDREHPACSPFGPRSKS 275

Query: 275 VTEVMKFPATLVLVGELDLLQDGQRRYYEVLKRMGKEVKMVEFKNAIHGFFAFWDLPQYS 334
           + E + FP +LV+V  LDL+QD Q +Y E LK+ G+EVK++  + A  GF+   +   + 
Sbjct: 276 L-EGLSFPKSLVVVAGLDLIQDWQLKYAEGLKKAGQEVKLLYLEQATIGFYLLPNNNHFH 335

Query: 335 SMIKEIKDFIATQFQ 340
           +++ EI  F+  + Q
Sbjct: 336 TVMDEIAAFVNAECQ 344

BLAST of PI0000428 vs. TAIR 10
Match: AT3G05120.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 238.4 bits (607), Expect = 8.5e-63
Identity = 130/312 (41.67%), Postives = 185/312 (59.29%), Query Frame = 0

Query: 35  RSDFTVNRWLTGILDWKIPSSTKPIDGVSSFDLTIDTSRNLWVRIFNPVIDDEDS----- 94
           R D T NR L   LD K+ ++  P+DGV SFD+ ID   NL  R++ P   D++      
Sbjct: 36  RPDGTFNRHLAEYLDRKVTANANPVDGVFSFDVLIDRRINLLSRVYRPAYADQEQPPSIL 95

Query: 95  ------NFQSLPVIFYFHGGGFALSYADSALSHTSAHRFAKQLPAVVISVNYRLAPEFRY 154
                 +   +PVI +FHGG FA S A+SA+  T   R       VV+SVNYR APE  Y
Sbjct: 96  DLEKPVDGDIVPVILFFHGGSFAHSSANSAIYDTLCRRLVGLCKCVVVSVNYRRAPENPY 155

Query: 155 PCQYDDGFDALKFIDEVEEEILPAKADLS-RCFILGESAGGNLGHHVAVRASEYALKKVK 214
           PC YDDG+ AL +++      L +K D     F+ G+S+GGN+ H+VA+RA E     + 
Sbjct: 156 PCAYDDGWIALNWVN--SRSWLKSKKDSKVHIFLAGDSSGGNIAHNVALRAGE---SGID 215

Query: 215 LVGFIASQPFFGGEERTESEIRLSNQRPLSLRLSDWFWKAFLPEGEDRDHVAANVFGPKG 274
           ++G I   P FGG ERTESE  L  +  +++R  DW+WKAFLPEGEDR+H A N F P+G
Sbjct: 216 VLGNILLNPMFGGNERTESEKSLDGKYFVTVRDRDWYWKAFLPEGEDREHPACNPFSPRG 275

Query: 275 RDVTEVMKFPATLVLVGELDLLQDGQRRYYEVLKRMGKEVKMVEFKNAIHGFFAFWDLPQ 334
           + + E + FP +LV+V  LDL++D Q  Y E LK+ G+EVK++  + A  GF+   +   
Sbjct: 276 KSL-EGVSFPKSLVVVAGLDLIRDWQLAYAEGLKKAGQEVKLMHLEKATVGFYLLPNNNH 335

BLAST of PI0000428 vs. TAIR 10
Match: AT3G63010.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 228.4 bits (581), Expect = 8.8e-60
Identity = 123/312 (39.42%), Postives = 187/312 (59.94%), Query Frame = 0

Query: 35  RSDFTVNRWLTGILDWKIPSSTKPIDGVSSFDLTIDTSRNLWVRIFNPV----------- 94
           R D + NR L   LD K+P+++ P+DGV SFD  +D++ NL  RI+ P            
Sbjct: 36  RPDGSFNRDLAEFLDRKVPANSFPLDGVFSFD-HVDSTTNLLTRIYQPASLLHQTRHGTL 95

Query: 95  -IDDEDSNFQSLPVIFYFHGGGFALSYADSALSHTSAHRFAKQLPAVVISVNYRLAPEFR 154
            +    S  + +PV+ +FHGG F  S A+SA+  T   R       VV+SV+YR +PE R
Sbjct: 96  ELTKPLSTTEIVPVLIFFHGGSFTHSSANSAIYDTFCRRLVTICGVVVVSVDYRRSPEHR 155

Query: 155 YPCQYDDGFDALKFIDEVEEEILPAKADLSRCFILGESAGGNLGHHVAVRASEYALKKVK 214
           YPC YDDG++AL ++ +    +   K      ++ G+S+GGN+ H+VAVRA+    + VK
Sbjct: 156 YPCAYDDGWNALNWV-KSRVWLQSGKDSNVYVYLAGDSSGGNIAHNVAVRATN---EGVK 215

Query: 215 LVGFIASQPFFGGEERTESEIRLSNQRPLSLRLSDWFWKAFLPEGEDRDHVAANVFGPKG 274
           ++G I   P FGG+ERT+SE  L  +  ++++  DW+W+A+LPEGEDRDH A N FGP+G
Sbjct: 216 VLGNILLHPMFGGQERTQSEKTLDGKYFVTIQDRDWYWRAYLPEGEDRDHPACNPFGPRG 275

Query: 275 RDVTEVMKFPATLVLVGELDLLQDGQRRYYEVLKRMGKEVKMVEFKNAIHGFFAFWDLPQ 334
           + +  V  FP +LV+V  LDL+QD Q  Y + LK+ G EV ++  K A  GF+   +   
Sbjct: 276 QSLKGV-NFPKSLVVVAGLDLVQDWQLAYVDGLKKTGLEVNLLYLKQATIGFYFLPNNDH 335

BLAST of PI0000428 vs. TAIR 10
Match: AT5G62180.1 (carboxyesterase 20 )

HSP 1 Score: 176.0 bits (445), Expect = 5.2e-44
Identity = 100/283 (35.34%), Postives = 160/283 (56.54%), Query Frame = 0

Query: 58  PIDGVSSFDLTIDTSRNLWVRIFNP--VIDDEDSNFQSLPVIFYFHGGGFALSYADSALS 117
           P++   S DL ++  ++ W+R++ P   +++ + + Q LP++ Y+HGGGF L   D  L 
Sbjct: 42  PLNPAVSKDLPVNQLKSTWLRLYLPSSAVNEGNVSSQKLPIVVYYHGGGFILCSVDMQLF 101

Query: 118 HTSAHRFAKQLPAVVISVNYRLAPEFRYPCQYDDGFDALKFIDEVEEEILPAKADLSRCF 177
           H      A+ L A+V+S +YRLAPE R P  YDDG +AL +I   ++E + + AD S  F
Sbjct: 102 HDFCSEVARDLNAIVVSPSYRLAPEHRLPAAYDDGVEALDWIKTSDDEWIKSHADFSNVF 161

Query: 178 ILGESAGGNLGHHVAVRA--SEYALKKVKLVGFIASQPFFGGEERTESEIRLSNQRPLSL 237
           ++G SAGGNL ++V +R+  S   L  +++ G I   PFFGGEER+ESEIRL N +    
Sbjct: 162 LMGTSAGGNLAYNVGLRSVDSVSDLSPLQIRGLILHHPFFGGEERSESEIRLMNDQVCPP 221

Query: 238 RLSDWFWKAFLPEGEDRDHVAAN-VFGPKGRDVTEVMKFPATLVLV-GELDLLQDGQRRY 297
            ++D  W   LP G DRDH  +N   G     + ++ +    ++++ GE D + D Q+  
Sbjct: 222 IVTDVMWDLSLPVGVDRDHEYSNPTVGDGSEKLEKIGRLRWKVMMIGGEDDPMIDLQKDV 281

Query: 298 YEVLKRMGKEVKMVEFKNAIHGFFAFWDLPQYSSMIKEIKDFI 335
            +++K+ G EV        +HG     D  +  ++   IK+FI
Sbjct: 282 AKLMKKKGVEVVEHYTGGHVHG-AEIRDPSKRKTLFLSIKNFI 323

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LT101.4e-7846.44Probable carboxylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=CXE18 PE=1 SV=1[more]
Q940G62.6e-6442.86Gibberellin receptor GID1C OS=Arabidopsis thaliana OX=3702 GN=GID1C PE=1 SV=1[more]
Q9MAA71.2e-6141.67Gibberellin receptor GID1A OS=Arabidopsis thaliana OX=3702 GN=GID1A PE=1 SV=1[more]
Q6L5452.9e-6041.07Gibberellin receptor GID1 OS=Oryza sativa subsp. japonica OX=39947 GN=GID1 PE=1 ... [more]
Q9LYC11.2e-5839.42Gibberellin receptor GID1B OS=Arabidopsis thaliana OX=3702 GN=GID1B PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3CAT67.0e-19093.93probable carboxylesterase 18 OS=Cucumis melo OX=3656 GN=LOC103498754 PE=4 SV=1[more]
A0A5D3BN571.6e-18993.93Putative carboxylesterase 18 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A0A0KUP39.5e-18792.51Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G6473... [more]
A0A6J1EWT78.7e-14877.58probable carboxylesterase 18 OS=Cucurbita moschata OX=3662 GN=LOC111438887 PE=4 ... [more]
A0A6J1IB872.5e-14777.27probable carboxylesterase 18 OS=Cucurbita maxima OX=3661 GN=LOC111471943 PE=4 SV... [more]
Match NameE-valueIdentityDescription
XP_008459712.11.4e-18993.93PREDICTED: probable carboxylesterase 18 [Cucumis melo][more]
KAA0039342.13.2e-18993.93putative carboxylesterase 18 [Cucumis melo var. makuwa] >TYK00525.1 putative car... [more]
XP_004141616.12.0e-18692.51probable carboxylesterase 18 [Cucumis sativus] >KGN52614.1 hypothetical protein ... [more]
XP_038890362.11.5e-17385.71probable carboxylesterase 18 [Benincasa hispida][more]
XP_022932482.11.8e-14777.58probable carboxylesterase 18 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT5G23530.11.0e-7946.44carboxyesterase 18 [more]
AT5G27320.11.8e-6542.86alpha/beta-Hydrolases superfamily protein [more]
AT3G05120.18.5e-6341.67alpha/beta-Hydrolases superfamily protein [more]
AT3G63010.18.8e-6039.42alpha/beta-Hydrolases superfamily protein [more]
AT5G62180.15.2e-4435.34carboxyesterase 20 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013094Alpha/beta hydrolase fold-3PFAMPF07859Abhydrolase_3coord: 97..315
e-value: 1.6E-52
score: 178.4
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 3..336
e-value: 5.4E-86
score: 290.9
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 48..336
NoneNo IPR availablePANTHERPTHR23024ARYLACETAMIDE DEACETYLASEcoord: 12..337
NoneNo IPR availablePANTHERPTHR23024:SF434ACETYL ESTERASEcoord: 12..337

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0000428.1PI0000428.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity