PI0000332 (gene) Melon (PI 482460) v1

BLAST of PI0000332 vs. ExPASy Swiss-Prot
Match: V9M2S5 (Disease resistance protein RPV1 OS=Vitis rotundifolia OX=103349 GN=RPV1 PE=1 SV=1)

HSP 1 Score: 471.9 bits (1213), Expect = 2.0e-131
Identity = 315/872 (36.12%), Postives = 483/872 (55.39%), Query Frame = 0

Query: 15  SFDVFLSFRGEDTRFNFTSHLDTALRQRGINVFIDNKLTRGANFPPSLLKAIEESKISVV 74
           ++DVFLSFRGEDTR+NFT HL +AL +RGI  F D++L RG    P LLKAIEES+ SV+
Sbjct: 23  TYDVFLSFRGEDTRYNFTDHLYSALGRRGIRTFRDDRLRRGEAIAPELLKAIEESRSSVI 82

Query: 75  IISENYASSSWCLNELVHIIMGNELRGQVVLPIFYKVNPSQVRTQNGAFGEAFAELEVRF 134
           + SENYA S WCL+ELV I+   +  G  V PIFY V+PS VR Q G+FGEAFA  E  +
Sbjct: 83  VFSENYAHSRWCLDELVKIMECQKDLGHAVFPIFYHVDPSHVRKQEGSFGEAFAGYEENW 142

Query: 135 FDKMQAWRDALTAISYMSGWVVLQKDDEANLIQKIVQQVWKKLTGSTMQLPVTKYPVGID 194
            DK+  WR ALT  + +SGW +L    E+N I++I   ++++L     +L V    VGID
Sbjct: 143 KDKIPRWRTALTEAANLSGWHLLDDRYESNQIKEITNSIFRQL--KCKRLDVGANLVGID 202

Query: 195 RQVENLL--SHVMIDGTRMVGLYGIGGMGKTTLAKALYNRIADDFEGCCFLANIREASKE 254
             V+ ++   H+     RMVG+YG+GG+GKTT+AK +YN ++ +FE   FL NIRE S  
Sbjct: 203 SHVKEMILRLHLESSDVRMVGIYGVGGIGKTTIAKVIYNELSCEFEYMSFLENIREGSNP 262

Query: 255 HEGLVRLQEKLLYEILMDDFIR-VGNLHRGINIIRNRLCSKKILLILDDIDTSVQLQALA 314
            + L  LQ +LL +IL  +  + + ++    ++I++ L S+++ ++LDD+D   QL+ L 
Sbjct: 263 -QVLFHLQNQLLGDILEGEGSQNISSVAHRASMIKDILLSRRVFIVLDDVDDLSQLEYLL 322

Query: 315 GGYDWFGYGSKVIVTTKNEQLLDIHGFNKLRSVPELNYDEALELFSWHAFQSSRPPSEYL 374
           G  +W G GS+VI+TT+N+ +L +   + L  V  LN++EA ELFS +AF+ + P S+Y 
Sbjct: 323 GHREWLGEGSRVIITTRNKHVLAVQEVDDLYEVEGLNFEEACELFSLYAFKQNLPKSDYR 382

Query: 375 QLSIDAVNYCKNLPLALEVLGSFLYSTDQSKFKGILEEFAISNLDKDIQNLLQVSYDELE 434
            L+   V YC+ LPLAL+VLGS L      +++G L++   S    DI  +L+ SYD L+
Sbjct: 383 NLTCRVVGYCQGLPLALKVLGSLLCKKTIPQWEGELKKLD-SEPKADIHKVLKRSYDGLD 442

Query: 435 GDVQEMFLFISCFFVGEDKTMVETMLKSCGCLCWERGIKKLMNLSLFTINKQNEVEMHDL 494
              + +FL ++CFF GE +  V  +L  C     E GI  L +L L T+   N++ MHDL
Sbjct: 443 RIDKNIFLDLACFFKGEGRDFVLRILDGCDFPA-ETGISNLNDLCLITL-PYNQICMHDL 502

Query: 495 IQQMGHTIARSKTSICPSEKKLLVG----DEAMHVLDGIK--EARAVKAIKLE------- 554
           IQQMG  I R    + P++   L      + A+   +GIK  E  ++   KL+       
Sbjct: 503 IQQMGWEIVRENFPVEPNKWSRLWDPCDFERALTADEGIKSVETMSLDLSKLKRVCSNSN 562

Query: 555 -FPKPTKLDI--------IDST---AFRKVKNLVVLKVKNVRSSKI-STLDFLPNSLRWM 614
            F K TKL +        IDS    +   ++ +  + +K+    ++  +  F    LR++
Sbjct: 563 VFAKMTKLRLLKVYSSSDIDSAHGDSDEDIEEVYDVVMKDASKMQLGQSFKFPSYELRYL 622

Query: 615 SWSEFPFSSFPSSYSMENLIQLKLPHSSIEHFGRAFMHCERLKQLDLSNSFFLEEIPDLS 674
            W  +P  S P ++    L++L L  S+I+   +     ERLK +DLS S  L ++ + S
Sbjct: 623 RWDGYPLDSLPLNFDGGKLVELHLKCSNIKQLWQGHKDLERLKVIDLSYSRKLSQMSEFS 682

Query: 675 AAINLENLSLSGCIGLVKVHKSVGSLGKLVDLSLSSHVYGFKQFPSPL-KLKSLKRFSTD 734
           +  NLE L LSGC+ L+ +H SVG++ KL  LSL S     K  P  +  L+SL+     
Sbjct: 683 SMPNLERLCLSGCVSLIDIHPSVGNMKKLTTLSLRS-CNKLKNLPDSIGDLESLESLYLS 742

Query: 735 HCTIVQGYPQFSEEMKSSLEDLWFYSSSITELSSTIRYLTSLKDLTITDCKKLTTLPSTI 794
           +C+  + +P+    MK SL +L   +++I +L  +I  L SL+ L +++C K    P   
Sbjct: 743 NCSKFEKFPEKGGNMK-SLTELDLKNTAIKDLPDSIGDLESLESLYLSNCSKFEKFPEKG 802

Query: 795 YDLSKLTSIEVSQSDFSTFPSSYSCPSSLTHL-----TRLHLYENKITNLDFLE------ 843
            ++  LT +++  +     P S     SL  L      +   +  K  N+  L+      
Sbjct: 803 GNMKSLTELDLKNTAIKDLPDSIGDLESLEILNLSDCAKFEKFPEKGGNMKSLKELDLQN 862

BLAST of PI0000332 vs. ExPASy Swiss-Prot
Match: V9M398 (Disease resistance protein RUN1 OS=Vitis rotundifolia OX=103349 GN=RUN1 PE=1 SV=1)

HSP 1 Score: 465.3 bits (1196), Expect = 1.8e-129
Identity = 332/903 (36.77%), Postives = 484/903 (53.60%), Query Frame = 0

Query: 3   RASGSFSSHFRCS------FDVFLSFRGEDTRFNFTSHLDTALRQRGINVFIDNKLTRGA 62
           RAS S SS    S      +DVFLSFRGEDTRFNFT HL +AL +RGI  F D+KL RG 
Sbjct: 8   RASSSSSSSSTPSIPRTITYDVFLSFRGEDTRFNFTDHLYSALGRRGIRTFRDDKLRRGE 67

Query: 63  NFPPSLLKAIEESKISVVIISENYASSSWCLNELVHIIMGNELR---GQVVLPIFYKVNP 122
              P LLKAIEES+ SV++ SENYA S WCL+ELV I+  ++ +   G  V PIFY V+P
Sbjct: 68  AIAPELLKAIEESRSSVIVFSENYARSRWCLDELVKIMECHKDKKDPGHAVFPIFYHVDP 127

Query: 123 SQVRTQNGAFGEAFAELEVRFFDKMQAWRDALTAISYMSGWVVLQKDDEANLIQKIVQQV 182
           S VR Q G+FGEAFA       DK+  WR ALT  + +SGW  LQ   E+N I++I   +
Sbjct: 128 SHVRKQEGSFGEAFAGYGENLKDKIPRWRTALTEAANLSGW-PLQDGYESNQIKEITDSI 187

Query: 183 WKKLTGSTMQLPVTKYPVGIDRQVENLL--SHVMIDGTRMVGLYGIGGMGKTTLAKALYN 242
           +++L     +L      VGID  V+ ++   H+     RMVG+YG+GG+GKTT+AK +YN
Sbjct: 188 FRRL--KCKRLDAGANLVGIDSHVKEMIWRLHMESSDVRMVGMYGVGGIGKTTIAKVIYN 247

Query: 243 RIADDFEGCCFLANIREASKEHEGLVRLQEKLLYEILMDDFIR-VGNLHRGINIIRNRLC 302
            ++ +FE   FL NIRE     +G+  LQ +LL +IL  +  + + ++  G ++I++ L 
Sbjct: 248 ELSREFEYMSFLENIREKFNT-QGVSPLQNQLLDDILKGEGSQNINSVAHGASMIKDILS 307

Query: 303 SKKILLILDDIDTSVQLQALAGGYDWFGYGSKVIVTTKNEQLLDIHGFNKLRSVPELNYD 362
           SK + ++LDD+D   QL+ L    +W G GS+VI+TT+N+ +LD+   + L  V  LN++
Sbjct: 308 SKIVFIVLDDVDDQSQLEYLLRHREWLGEGSRVIITTRNKHVLDVQKVDDLYEVKGLNFE 367

Query: 363 EALELFSWHAFQSSRPPSEYLQLSIDAVNYCKNLPLALEVLGSFLYSTDQSKFKGILEEF 422
           EA ELFS +AF+ + P S+Y  LS   V YC+ LPLAL+VLG  L      +++  L + 
Sbjct: 368 EACELFSLYAFEQNLPKSDYRNLSHRVVGYCQGLPLALKVLGCLLLKKTIPEWESELRKL 427

Query: 423 AISNLDKDIQNLLQVSYDELEGDVQEMFLFISCFFVGEDKTMVETMLKSCGCLCWERGIK 482
                + +I ++L+ SYD L    + +FL ++CFF GED+  V  +L +C     E GIK
Sbjct: 428 D-REPEAEILSVLKRSYDGLGRTEKSIFLDVACFFKGEDRDFVSKILDACDFHA-EIGIK 487

Query: 483 KLMNLSLFTINKQNEVEMHDLIQQMGHTIARSKTSICPSE-KKLLVGDEAMHVLDGIKEA 542
            L +  L T+ + N + MHDLIQQMG  I R K    P++  +L    +    L   K  
Sbjct: 488 NLNDKCLITL-QYNRIRMHDLIQQMGWEIVREKFPDEPNKWSRLWDTCDFERALTAYKGI 547

Query: 543 RAVKAIKLEFPKPTKLDIIDSTAFRKVKNLVVLKV------------------------- 602
           + V+ I L+  K  K    +S AF K+  L +LKV                         
Sbjct: 548 KRVETISLDLSK-LKRVCSNSNAFAKMTRLRLLKVQSSLDIDFEPEYIDADDKVELYDVV 607

Query: 603 -KNVRSSKIST-LDFLPNSLRWMSWSEFPFSSFPSSYSMENLIQLKLPHSSIEHFGRAFM 662
            KN    ++     F    LR++ W  +P    PS++    L++L L  S+I+       
Sbjct: 608 MKNASKMRLGRGFKFPSYELRYLRWDGYPLDFLPSNFDGGKLVELHLKCSNIKQLRLGNK 667

Query: 663 HCERLKQLDLSNSFFLEEIPDLSAAINLENLSLSGCIGLVKVHKSVGSLGKLVDLSLSSH 722
             E LK +DLS S  L ++ + S+  NLE L L GC+ L+ +H SVG++ KL  LSL S 
Sbjct: 668 DLEMLKVIDLSYSRKLSQMSEFSSMPNLERLFLRGCVSLIDIHPSVGNMKKLTTLSLKS- 727

Query: 723 VYGFKQFPSPL-KLKSLKRFSTDHCTIVQGYPQFSEEMKSSLEDLWFYSSSITELSSTIR 782
               K  P  +  L+SL+     +C+  + +P+    MK SL +L   +++I +L  +I 
Sbjct: 728 CKKLKNLPDSIGDLESLEILDLAYCSKFEKFPEKGGNMK-SLTELDLQNTAIKDLPDSIG 787

Query: 783 YLTSLKDLTITDCKKLTTLPSTIYDLSKLTSIEVSQSDFSTFPSSYSCPSSLTHLTRLHL 842
            L SLK L ++DC K    P    ++  L  +++  +     P S      L  L RL+L
Sbjct: 788 DLESLKYLDLSDCSKFEKFPEKGGNMKSLRELDLRNTAIKDLPDSI---RDLESLERLYL 847

Query: 843 -YENKITNLDFLETIAHVAPSLRELNLFLETFDCKLLEEIPKIPEGLISLGAEGCISLAK 864
            Y +K     F E   ++  SL EL+  L+    K L +     E L  L    C    K
Sbjct: 848 SYCSKFEK--FPEKGGNM-KSLMELD--LQNTAIKDLPDSIGDLESLKYLDLSNCSKFEK 892

BLAST of PI0000332 vs. ExPASy Swiss-Prot
Match: A0A290U7C4 (Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV=1)

HSP 1 Score: 459.5 bits (1181), Expect = 1.0e-127
Identity = 301/874 (34.44%), Postives = 484/874 (55.38%), Query Frame = 0

Query: 9   SSHFRCSFDVFLSFRGEDTRFNFTSHLDTALRQRGINVFIDNK-LTRGANFPPSLLKAIE 68
           SSH   S+DVFLSFRGEDTR  F  HL  AL ++GI+ F+D+K L RG +    L+KAI 
Sbjct: 5   SSHHGRSYDVFLSFRGEDTRKTFVGHLFNALIEKGIHTFMDDKELKRGKSISSELMKAIG 64

Query: 69  ESKISVVIISENYASSSWCLNELVHIIMGNELRGQVVLPIFYKVNPSQVRTQNGAFGEAF 128
           ES+ +VV+ S+NYASS+WCL ELV I+  +E    +V+P+FY V+PS VR QNG +   F
Sbjct: 65  ESRFAVVVFSKNYASSTWCLEELVKILEIHEKFELIVVPVFYDVDPSTVRKQNGEYAVCF 124

Query: 129 AELEVRFF---DKMQAWRDALTAISYMSGWVV--LQKDDEANLIQKIVQQVWKKLTGSTM 188
            + E       DK+  WR+ALT ++ +SG  +      DE+  IQ+I++ ++ K   S  
Sbjct: 125 TKFEANLVDDRDKVLRWREALTKVANISGHDLRNTYNGDESKCIQQILKDIFDKFCFS-- 184

Query: 189 QLPVTKYP-VGIDRQVENLLSHVMID--GTRMVGLYGIGGMGKTTLAKALYNRIADDFEG 248
            + +T    VGI+ Q++ L S + +D  G R+VG++G+GG+GKTT A+AL+NR   +FE 
Sbjct: 185 -ISITNRDLVGIESQIKKLSSLLRMDLKGVRLVGIWGMGGVGKTTAARALFNRYYQNFES 244

Query: 249 CCFLANIREASKEHEGLVRLQEKLLYEILMDDFIRVGNLHRGINIIRNRLCSKKILLILD 308
            CFL +++E   +H  L+ LQ+ LL ++L  +F+   +      I++ RLCSKK+L++LD
Sbjct: 245 ACFLEDVKE-YLQHHTLLYLQKTLLSKLLKVEFVDCTDTEEMCVILKRRLCSKKVLVVLD 304

Query: 309 DIDTSVQLQALAGGYDWFGYGSKVIVTTKNEQLLDIHGFNKLRSVPELNYDEALELFSWH 368
           D++ + QL  L G  DWFG GS++++TT++ +LL  H  ++   +  L  DEA+ELF+ H
Sbjct: 305 DVNHNDQLDKLVGAEDWFGSGSRIVITTRDMKLLKNHDVHETYEIKVLEKDEAIELFNLH 364

Query: 369 AFQSSRPPSEYLQLSIDAVNYCKNLPLALEVLGSFLYSTDQSKFKGILEEFAISNLDKDI 428
           AF+ S P  E+ +L    V+Y   LPLAL+VLGS LY  D   +   ++     N + +I
Sbjct: 365 AFKRSSPEKEFKELLNLVVDYTGGLPLALKVLGSLLYKEDLDVWISTIDRLK-DNPEGEI 424

Query: 429 QNLLQVSYDELEGDVQEMFLFISCFFVGEDKTMVETMLKSCGCLCWERGIKKLMNLSLFT 488
              L++S+D L    + +FL I+CFF G ++  +  +  + G      G+K L+  SL  
Sbjct: 425 MATLKISFDGLRDYEKSIFLDIACFFRGYNQRDMTALFHASG-FHPVLGVKTLVEKSLIF 484

Query: 489 INKQNEVEMHDLIQQMGHTIARSKTSI----CPSE-KKLLVGDEAMHVLDG--IKEARAV 548
           I  +++++MHDL+Q+MG  IA  ++ +     P + K   +GD     ++G  + E    
Sbjct: 485 I-LEDKIQMHDLMQEMGRQIAVQESPMRRIYRPEDVKDACIGDMRKEAIEGLLLTEPEQF 544

Query: 549 KAIKLEFPKPTKLDIIDSTAFRKVKNLVVLKVKNVRSSKISTLDFLPNSLRWMSWSEFPF 608
           +  +LE+       +  + A +K + L +L  +         + +LPNSL W+ W  +  
Sbjct: 545 EEGELEY-------MYSAEALKKTRRLRILVKEYYNRGFDEPVAYLPNSLLWLEWRNYSS 604

Query: 609 SSFPSSYSMENLIQLKLPHSSIEHFGRAFMHCERLKQLDLSNSFFLEEIPDLSAAINLEN 668
           +SFPS++    L+ L +  SSI            L  LDLS    L + PD     NLE 
Sbjct: 605 NSFPSNFEPSKLVYLTMKGSSIIELWNGAKRLAFLTTLDLSYCHKLIQTPDFRMITNLER 664

Query: 669 LSLSGCIGLVKVHKSVGSLGKLVDLSLSSHVYGFKQFPSPLKLKSLKRFSTDHCTIVQGY 728
           L LS C  LV+VH SVG L  L+ L++  H    ++ P+ ++ + L+    ++C  ++ +
Sbjct: 665 LILSSCDALVEVHPSVGFLKNLILLNM-DHCISLERLPAIIQSECLEVLDLNYCFNLKMF 724

Query: 729 PQFSEEMKSSLEDLWFYSSSITELSSTIRYLTSLKDLTITDCKKLTTLPSTIYDLSKLTS 788
           P+    M + L+ L   S+ I EL ++I +L+SL++L +  C +L +LPS+I+    L  
Sbjct: 725 PEVERNM-THLKKLDLTSTGIRELPASIEHLSSLENLQMHSCNQLVSLPSSIWRFRNLKI 784

Query: 789 IEVSQ-SDFSTFPSSYSCPS----SLTHLTRLHLYENKITNLDFLE-----TIAHVAPSL 848
            E  +         + +C       L  +  L      +T+L+FLE     TI+ ++ S+
Sbjct: 785 SECEKLGSLPEIHGNSNCTRELILKLVSIKELPTSIGNLTSLNFLEICNCKTISSLSSSI 844

Query: 849 RELNLF--LETFDCKLLEEIPKIPEGLISLGAEG 855
             L     L+  DC+ L+ +P IP  +  L   G
Sbjct: 845 WGLTSLTTLKLLDCRKLKNLPGIPNAINHLSGHG 862

BLAST of PI0000332 vs. ExPASy Swiss-Prot
Match: Q40392 (TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1)

HSP 1 Score: 446.8 bits (1148), Expect = 6.8e-124
Identity = 312/860 (36.28%), Postives = 478/860 (55.58%), Query Frame = 0

Query: 7   SFSSHFRCSFDVFLSFRGEDTRFNFTSHLDTALRQRGINVFIDNK-LTRGANFPPSLLKA 66
           S SS  R S+DVFLSFRGEDTR  FTSHL   L  +GI  F D+K L  GA  P  L KA
Sbjct: 3   SSSSSSRWSYDVFLSFRGEDTRKTFTSHLYEVLNDKGIKTFQDDKRLEYGATIPGELCKA 62

Query: 67  IEESKISVVIISENYASSSWCLNELVHIIMGNELRGQVVLPIFYKVNPSQVRTQNGAFGE 126
           IEES+ ++V+ SENYA+S WCLNELV I+       Q V+PIFY V+PS VR Q  +F +
Sbjct: 63  IEESQFAIVVFSENYATSRWCLNELVKIMECKTRFKQTVIPIFYDVDPSHVRNQKESFAK 122

Query: 127 AFAELEVRFFDK---MQAWRDALTAISYMSGWVVLQKDDEANLIQKIVQQVWKKLTGSTM 186
           AF E E ++ D    +Q WR AL   + + G    +   +A+ I++IV Q+  KL    +
Sbjct: 123 AFEEHETKYKDDVEGIQRWRIALNEAANLKGSCDNRDKTDADCIRQIVDQISSKL--CKI 182

Query: 187 QLPVTKYPVGIDRQVENL--LSHVMIDGTRMVGLYGIGGMGKTTLAKALYNRI------A 246
            L   +  VGID  +E +  L  + I+G R++G++G+GG+GKTT+A+A+++ +      +
Sbjct: 183 SLSYLQNIVGIDTHLEKIESLLEIGINGVRIMGIWGMGGVGKTTIARAIFDTLLGRMDSS 242

Query: 247 DDFEGCCFLANIREASKEHEGLVRLQEKLLYEILMDDFIRVGNLHRGINIIRNRLCSKKI 306
             F+G CFL +I+E  +   G+  LQ  LL E+L +      N   G + + +RL SKK+
Sbjct: 243 YQFDGACFLKDIKENKR---GMHSLQNALLSELLREK-ANYNNEEDGKHQMASRLRSKKV 302

Query: 307 LLILDDIDTSVQ-LQALAGGYDWFGYGSKVIVTTKNEQLLDIHGFNKLRSVPELNYDEAL 366
           L++LDDID     L+ LAG  DWFG GS++I+TT+++ L++ +    +  V  L   E++
Sbjct: 303 LIVLDDIDNKDHYLEYLAGDLDWFGNGSRIIITTRDKHLIEKNDI--IYEVTALPDHESI 362

Query: 367 ELFSWHAFQSSRPPSEYLQLSIDAVNYCKNLPLALEVLGSFLYSTDQSKFKGILEEFAIS 426
           +LF  HAF    P   + +LS++ VNY K LPLAL+V GS L++   +++K  +E    +
Sbjct: 363 QLFKQHAFGKEVPNENFEKLSLEVVNYAKGLPLALKVWGSLLHNLRLTEWKSAIEHMK-N 422

Query: 427 NLDKDIQNLLQVSYDELEGDVQEMFLFISCFFVGEDKTMVETMLKSCGCLCWERGIKKLM 486
           N    I + L++SYD LE   QEMFL I+CF  GE+K  +  +L+SC  +  E G++ L+
Sbjct: 423 NSYSGIIDKLKISYDGLEPKQQEMFLDIACFLRGEEKDYILQILESCH-IGAEYGLRILI 482

Query: 487 NLSLFTINKQNEVEMHDLIQQMGHTIARSKTSICPSEK-KLLVGDEAMHVLDGIKEARAV 546
           + SL  I++ N+V+MHDLIQ MG  I   +    P E+ +L +  E   V+       A+
Sbjct: 483 DKSLVFISEYNQVQMHDLIQDMGKYIVNFQKD--PGERSRLWLAKEVEEVMSNNTGTMAM 542

Query: 547 KAIKLEFPKPTKLDIIDSTAFRKVKNLVVLKVKNV-RSSKISTLDFLPNSLRWMSWSEFP 606
           +AI +     T        + + VKN+  L+V N+ RSS    +D+LPN+LR    + +P
Sbjct: 543 EAIWVSSYSSTL-----RFSNQAVKNMKRLRVFNMGRSSTHYAIDYLPNNLRCFVCTNYP 602

Query: 607 FSSFPSSYSMENLIQLKLPHSSIEHFGRAFMHCERLKQLDLSNSFFLEEIPDLSAAINLE 666
           + SFPS++ ++ L+ L+L H+S+ H      H   L+++DLS S  L   PD +   NLE
Sbjct: 603 WESFPSTFELKMLVHLQLRHNSLRHLWTETKHLPSLRRIDLSWSKRLTRTPDFTGMPNLE 662

Query: 667 NLSLSGCIGLVKVHKSVGSLGKLVDLSLSSHVYGFKQFPSPLKLKSLKRFSTDHCTIVQG 726
            ++L  C  L +VH S+G   K++ L L+      K+FP  + ++SL+      C  ++ 
Sbjct: 663 YVNLYQCSNLEEVHHSLGCCSKVIGLYLND-CKSLKRFPC-VNVESLEYLGLRSCDSLEK 722

Query: 727 YPQFSEEMKSSLEDLWFYSSSITELSSTI-RYLTSLKDLTITDCKKLTTLPSTIYDLSKL 786
            P+    MK  ++ +    S I EL S+I +Y T +  L + + K L  LPS+I  L  L
Sbjct: 723 LPEIYGRMKPEIQ-IHMQGSGIRELPSSIFQYKTHVTKLLLWNMKNLVALPSSICRLKSL 782

Query: 787 TSIEVSQSDFSTFPSSYSCPSSLTHLTRLHLYENKITNLDFLETIAHVAPSLRELNLFLE 846
            S+ VS           S P  +  L  L +++   T +  L   + +    + + L   
Sbjct: 783 VSLSVSGCS-----KLESLPEEIGDLDNLRVFDASDTLI--LRPPSSIIRLNKLIILMFR 835

Query: 847 TFDCKLLEEIPKIPEGLISL 851
            F   +  E P + EGL SL
Sbjct: 843 GFKDGVHFEFPPVAEGLHSL 835

BLAST of PI0000332 vs. ExPASy Swiss-Prot
Match: F4I594 (Disease resistance protein RML1A OS=Arabidopsis thaliana OX=3702 GN=RLM1A PE=3 SV=1)

HSP 1 Score: 401.7 bits (1031), Expect = 2.5e-110
Identity = 293/975 (30.05%), Postives = 490/975 (50.26%), Query Frame = 0

Query: 4   ASGSFSSHFRCSFDVFLSFRGEDTRFNFTSHLDTALRQRGINVFIDNKLTRGANFPPSLL 63
           AS S S+     + VF SF G D R +F SH        GI +F D ++ RG    P+L 
Sbjct: 2   ASSSSSASRTWRYRVFTSFHGSDVRTSFLSHFRKQFNNNGITMFDDQRILRGETISPALT 61

Query: 64  KAIEESKISVVIISENYASSSWCLNELVHIIMGNELRGQVVLPIFYKVNPSQVRTQNGAF 123
           +AI ES+IS+V++S+NYASS WCL+EL+ I+   +  GQ+V+ +FY V+PS VR Q G F
Sbjct: 62  QAIRESRISIVLLSKNYASSGWCLDELLEILKCKDDMGQIVMTVFYGVDPSDVRKQTGEF 121

Query: 124 GEAFAE-LEVRFFDKMQAWRDALTAISYMSGWVVLQKDDEANLIQKIVQQVWKKLTGSTM 183
           G AF E    R  ++ Q W  AL  +  ++G  +L  D+EA +I+KI + V +KL  +  
Sbjct: 122 GIAFNETCACRTEEERQKWSQALNYVGNIAGEHLLNWDNEAKMIEKIARDVSEKLNVT-- 181

Query: 184 QLPVTKYP--VGIDRQVENLLSHVMIDG--TRMVGLYGIGGMGKTTLAKALYNRIADDFE 243
             P   +   VGI+  +  + S + +D    +MV + G  G+GK+T+ +AL++ +++ F 
Sbjct: 182 --PCRDFDGMVGIEAHLRKIQSLLDLDNDEVKMVAISGPAGIGKSTIGRALHSLLSNRFH 241

Query: 244 GCCFLANIREAS----KEHEGLVRLQEKLLYEILMDDFIRVGNLHRGINIIRNRLCSKKI 303
             CF+ N+R +      E+   +RLQE+LL +IL  D  R+ +L      I+ RLC  K+
Sbjct: 242 HTCFVDNLRGSHPIGLDEYGLKLRLQEQLLSKILNQDGSRICHL----GAIKERLCDMKV 301

Query: 304 LLILDDIDTSVQLQALAGGYDWFGYGSKVIVTTKNEQLLDIHGFNKLRSVPELNYDEALE 363
            +ILDD++   QL+ALA   +WFG GS++IVTT+N++LL  HG N    V   + +EA++
Sbjct: 302 FIILDDVNDVKQLEALANESNWFGPGSRIIVTTENKELLKQHGINNTYYVGFPSDEEAIK 361

Query: 364 LFSWHAFQSSRPPSEYLQLSIDAVNYCKNLPLALEVLGSFLYSTDQSKFKGILEEFAISN 423
           +   +AF+ S     + +L+      C  LPL L V+GS L+  ++ +++ ++     + 
Sbjct: 362 ILCRYAFRQSSSRHGFKKLTRSVTELCGKLPLGLRVVGSSLHGKNEEEWEYVIRRLE-TI 421

Query: 424 LDKDIQNLLQVSYDELEGDVQEMFLFISCFFVGEDKTMVETMLKSCGCLCWERGIKKLMN 483
           +D+DI+ +L+V Y+ L  + Q +FL I+ FF  ED  +V+ ML     L  E  +  L+N
Sbjct: 422 IDRDIEQVLRVGYESLHENEQSLFLHIAIFFNYEDGDLVKAMLAE-NDLDIEHELNILVN 481

Query: 484 LSLFTINKQNEVEMHDLIQQMGHTIARSKTSICPSEKKLLV-GDEAMHVLDGIKEARAVK 543
            SL  I+    + MH L+Q +G    + +    P ++++L+   E  HVL+      AV 
Sbjct: 482 KSLIYISTDGRIRMHKLLQLVGRQANQREE---PWKRRILIDAQEICHVLENDIGTGAVS 541

Query: 544 AIKLEFPKPTKLDIIDSTAFRKVKNLVVLKVKNVRSSKISTLDF-----LPNSLRWMSWS 603
            I  +     ++  I + A R++ NL  L V   +    + +D       P  LR + W 
Sbjct: 542 GILFDTSGINEVS-ISNKALRRMCNLRFLSVYKTKHDGYNRMDIPEDMEFPPRLRLLHWD 601

Query: 604 EFPFSSFPSSYSMENLIQLKLPHSSIEHFGRAFMHCERLKQLDLSNSFFLEEIPDLSAAI 663
            +P    P  +  ENL++L +  S +E+         +LK+L+L  S+ L+E+PDLS A 
Sbjct: 602 AYPSKCLPLKFRAENLVELDMKDSRLEYLWPGTQLLTKLKKLNLEGSYNLKELPDLSNAT 661

Query: 664 NLENLSLSGCIGLVKVHKSVGSLGKL----VDLSLSSHVYGFKQFPSPLKLKSLKRFSTD 723
           NLE L LS C+ L ++  S+ +L KL    +DL  S H+      P+ + L SL+     
Sbjct: 662 NLEMLDLSVCLALAELPSSIKNLHKLDVIYMDLCESLHM-----IPTNINLASLETMYMT 721

Query: 724 HCTIVQGYPQFSEEMKSSLEDLWFYSSSITELSSTIRYLTSLKDLTIT---DCKKLTTLP 783
            C  ++ +P FS ++K     L+   + + E+ ++I + + L  + ++   + K +T LP
Sbjct: 722 GCPQLKTFPAFSTKIKR----LYLVRTGVEEVPASITHCSRLLKIDLSGSRNLKSITHLP 781

Query: 784 STIYDLSKLTSIEVSQSDFSTFPSSYSCPSSLTHLTRLHLYENKITNLDFLETIAHVAPS 843
           S+      L ++++S +D        SC   L  L  L L                    
Sbjct: 782 SS------LQTLDLSSTDIEMIAD--SCIKDLQRLDHLRLCR------------------ 841

Query: 844 LRELNLFLETFDCKLLEEIPKIPEGLISLGAEGCISLAKF------PDNLADFISC---- 903
                       C+ L+ +P++P  L  L AE C SL +       P    +F +C    
Sbjct: 842 ------------CRKLKSLPELPASLRLLTAEDCESLERVTYPLNTPTGQLNFTNCLKLG 901

Query: 904 -DSEHV---DGQFKQLILMNCDIPDWFSYKSRNNPMTIFMPSNDPRWEFKVFAPCVKFQV 943
            +++ V       K        +P  F++++R N + I + S+   + FK        Q+
Sbjct: 902 EEAQRVIIQQSLVKHACFPGSVMPSEFNHRARGNSLKILVKSS-ASFAFKACVLISPRQL 914

BLAST of PI0000332 vs. ExPASy TrEMBL
Match: A0A0A0LFP7 (TIR domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G021510 PE=4 SV=1)

HSP 1 Score: 1888.6 bits (4891), Expect = 0.0e+00
Identity = 952/1067 (89.22%), Postives = 993/1067 (93.06%), Query Frame = 0

Query: 1    MDRASGSFSSH-FRCSFDVFLSFRGEDTRFNFTSHLDTALRQRGINVFIDNKLTRGANFP 60
            MDRASGS SS  FR +FDVFLSFRGEDTR NFTSHL+ ALRQRGINVFIDN+++RG    
Sbjct: 1    MDRASGSSSSSCFRWTFDVFLSFRGEDTRSNFTSHLNMALRQRGINVFIDNRISRGQEIS 60

Query: 61   PSLLKAIEESKISVVIISENYASSSWCLNELVHIIMGNELRGQVVLPIFYKVNPSQVRTQ 120
             SL +AIEESKIS+VIIS+NYASSSWCLNELV IIM  ELRGQVVLPIFYKVNPSQVR Q
Sbjct: 61   ASLFEAIEESKISIVIISQNYASSSWCLNELVKIIMCKELRGQVVLPIFYKVNPSQVRKQ 120

Query: 121  NGAFGEAFAELEVRFFDKMQAWRDALTAISYMSGWVVLQKDDEANLIQKIVQQVWKKLTG 180
            NGAFGEAFAELEVRFFDKMQAW +ALTA+S+MSGWVVL+KDDEANLIQKIVQQVWKKLT 
Sbjct: 121  NGAFGEAFAELEVRFFDKMQAWGEALTAVSHMSGWVVLEKDDEANLIQKIVQQVWKKLTC 180

Query: 181  STMQLPVTKYPVGIDRQVENLLSHVMIDGTRMVGLYGIGGMGKTTLAKALYNRIADDFEG 240
            STMQLPVTKYPVGIDRQ ENLLSHVMIDGTRMVGL+GIGGMGKTTLAK LYNRIADDFEG
Sbjct: 181  STMQLPVTKYPVGIDRQFENLLSHVMIDGTRMVGLHGIGGMGKTTLAKTLYNRIADDFEG 240

Query: 241  CCFLANIREASKEHEGLVRLQEKLLYEILMDDFIRVGNLHRGINIIRNRLCSKKILLILD 300
            CCFLANIREASK+HEGLVRLQEKLLYEILMDDFIRV +L++GINIIRNRLCSKKILLILD
Sbjct: 241  CCFLANIREASKQHEGLVRLQEKLLYEILMDDFIRVSDLYKGINIIRNRLCSKKILLILD 300

Query: 301  DIDTSVQLQALAGGYDWFGYGSKVIVTTKNEQLLDIHGFNKLRSVPELNYDEALELFSWH 360
            DIDTS QLQ LAGGYDWFGYGSKVIVTT+NE LLDIHGFNKLRSVPELNY EALELFSWH
Sbjct: 301  DIDTSEQLQVLAGGYDWFGYGSKVIVTTRNEHLLDIHGFNKLRSVPELNYGEALELFSWH 360

Query: 361  AFQSSRPPSEYLQLSIDAVNYCKNLPLALEVLGSFLYSTDQSKFKGILEEFAISNLDKDI 420
            AFQ S PP+EYLQLS DAVNYCKNLPLALEVLGSFLYSTDQSKFKGILEEFAISNLDKDI
Sbjct: 361  AFQCSSPPTEYLQLSKDAVNYCKNLPLALEVLGSFLYSTDQSKFKGILEEFAISNLDKDI 420

Query: 421  QNLLQVSYDELEGDVQEMFLFISCFFVGEDKTMVETMLKSCGCLCWERGIKKLMNLSLFT 480
            QNLLQVSYDELEGDVQEMFLFISCFFVGEDKTMVETMLKSCGCLCWE+GI+KLMNLSL T
Sbjct: 421  QNLLQVSYDELEGDVQEMFLFISCFFVGEDKTMVETMLKSCGCLCWEKGIQKLMNLSLLT 480

Query: 481  INKQNEVEMHDLIQQMGHTIARSKTSICPSEKKLLVGDEAMHVLDGIKEARAVKAIKLEF 540
            IN+ N+VEMHDLIQQ+GHTIARSKTSI PSEKKLLVGD+AMHVLDGIK+ARAVKAIKLEF
Sbjct: 481  INQWNKVEMHDLIQQLGHTIARSKTSISPSEKKLLVGDDAMHVLDGIKDARAVKAIKLEF 540

Query: 541  PKPTKLDIIDSTAFRKVKNLVVLKVKNVRSSKISTLDFLPNSLRWMSWSEFPFSSFPSSY 600
            PKPTKLDIIDSTAFRKVKNLVVLKVKNV S KISTLDFLPNSLRWMSWSEFPFSSFPSSY
Sbjct: 541  PKPTKLDIIDSTAFRKVKNLVVLKVKNVISPKISTLDFLPNSLRWMSWSEFPFSSFPSSY 600

Query: 601  SMENLIQLKLPHSSIEHFGRAFMHCERLKQLDLSNSFFLEEIPDLSAAINLENLSLSGCI 660
            SMENLIQLKLPHS+I+HFGRAFMHCERLKQLDLSNSFFLEEIPDLSAAINLENLSLSGCI
Sbjct: 601  SMENLIQLKLPHSAIQHFGRAFMHCERLKQLDLSNSFFLEEIPDLSAAINLENLSLSGCI 660

Query: 661  GLVKVHKSVGSLGKLVDLSLSSHVYGFKQFPSPLKLKSLKRFSTDHCTIVQGYPQFSEEM 720
             LVKVHKSVGSL KL+DLSLSSHVYGFKQFPSPL+LKSLKRFSTDHCTI+QGYPQFS+EM
Sbjct: 661  SLVKVHKSVGSLPKLIDLSLSSHVYGFKQFPSPLRLKSLKRFSTDHCTILQGYPQFSQEM 720

Query: 721  KSSLEDLWFYSSSITELSSTIRYLTSLKDLTITDCKKLTTLPSTIYDLSKLTSIEVSQSD 780
            KSSLEDLWF SSSIT+LSSTIRYLTSLKDLTI DCKKLTTLPSTIYDLSKLTSIEVSQSD
Sbjct: 721  KSSLEDLWFQSSSITKLSSTIRYLTSLKDLTIVDCKKLTTLPSTIYDLSKLTSIEVSQSD 780

Query: 781  FSTFPSSYSCPSSLTHLTRLHLYENKITNLDFLETIAHVAPSLRELNLFLETFDCKLLEE 840
             STFPSSYSCPSSL  LTRLHLYENKITNLDFLETIAH APSLRELN  L + D + L  
Sbjct: 781  LSTFPSSYSCPSSLPLLTRLHLYENKITNLDFLETIAHAAPSLRELN--LNSKDSRRLNF 840

Query: 841  IPKIPEGLISLGAEGCISLAKFPDNLADFISCDSEHVDGQFKQLILMNCDIPDWFSYKSR 900
                          GCISLAK PDNLADFISCDSEHVDG FKQLILMNCDIPDWFSYKSR
Sbjct: 841  F-------------GCISLAKSPDNLADFISCDSEHVDGHFKQLILMNCDIPDWFSYKSR 900

Query: 901  NNPMTIFMPSNDPRWEFKVFAPCVKFQVNHVDRNRYMDLECKVLINDIQVWSSEEVPFLE 960
            NN +TIFMPSNDP WE KVFAPCVKFQVNHVDRNRYMDLECK+ INDIQVWSSEEVP LE
Sbjct: 901  NNHITIFMPSNDPSWEMKVFAPCVKFQVNHVDRNRYMDLECKMFINDIQVWSSEEVPCLE 960

Query: 961  ESRRILIEASPYEYMWLLVLYPHIDFPLNWDDIIKRSQE-FNLHQPSFGINSMGRDYNNC 1020
            ESRRILIE SPYEYMWLLVLYPHIDFPLN +DI+KRSQE FNLHQPSFGINSMGRD NNC
Sbjct: 961  ESRRILIEESPYEYMWLLVLYPHIDFPLNSEDIVKRSQEYFNLHQPSFGINSMGRDNNNC 1020

Query: 1021 DVDDDRRNHFGESIWRKFTVSFGVNSKFKDSEVSIKRCGVHVIMEEW 1066
            +VDDDRRNHFGESIWRKFTVSFGVNSKFKDS+VSIKRCGVHVIMEEW
Sbjct: 1021 NVDDDRRNHFGESIWRKFTVSFGVNSKFKDSQVSIKRCGVHVIMEEW 1052

BLAST of PI0000332 vs. ExPASy TrEMBL
Match: A0A1S4DZW3 (TMV resistance protein N-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103495278 PE=4 SV=1)

HSP 1 Score: 1786.9 bits (4627), Expect = 0.0e+00
Identity = 905/1089 (83.10%), Postives = 978/1089 (89.81%), Query Frame = 0

Query: 1    MDRASGSFSSHFRCSFDVFLSFRGEDTRFNFTSHLDTALRQRGINVFI-DNKLTRGANFP 60
            MDRASGSFSSH R  FDVFLSFRGEDTRFNFTSHL TALRQRGINVFI D++LTRG NFP
Sbjct: 1    MDRASGSFSSH-RWRFDVFLSFRGEDTRFNFTSHLYTALRQRGINVFIDDSELTRGENFP 60

Query: 61   PSLLKAIEESKISVVIISENYASSSWCLNELVHIIMGNELRGQVVLPIFYKVNPSQVRTQ 120
             SLL+AIEESKISVVIISENYA+SSWCLNELV++IM  +LRGQVVLPIFYKVNPSQVRTQ
Sbjct: 61   SSLLRAIEESKISVVIISENYATSSWCLNELVYLIMCKKLRGQVVLPIFYKVNPSQVRTQ 120

Query: 121  NGAFGEAFAELEVRFFDKMQAWRDALTAISYMSGWVVLQKDDEANLIQKIVQQVWKKLTG 180
            NGAFGEAFA+LEVRFFDKMQAWR+ALT +S+MSGWV+LQ DDEA LIQ IV+ VWKKLT 
Sbjct: 121  NGAFGEAFAKLEVRFFDKMQAWREALTTVSFMSGWVLLQNDDEARLIQIIVRHVWKKLTC 180

Query: 181  STMQLPVTKYPVGIDRQVENLLSHVMIDGTRMVGLYGIGGMGKTTLAKALYNRIADDFEG 240
            ST+QL VTKYPVGIDRQV++LLSHV+ID TRMVGLYGIGGMGKTTLAKALYNR+AD FEG
Sbjct: 181  STVQLSVTKYPVGIDRQVKDLLSHVIIDETRMVGLYGIGGMGKTTLAKALYNRVADKFEG 240

Query: 241  CCFLANIREASKEHEGLVRLQEKLLYEILMDDFIRVGNLHRGINIIRNRLCSKKILLILD 300
            CCFLANIREASK+H+GLVRLQEKLLY+ILM DF+RVG++++GINIIRNRL S +ILLILD
Sbjct: 241  CCFLANIREASKQHDGLVRLQEKLLYDILMYDFVRVGDVYKGINIIRNRLYSIRILLILD 300

Query: 301  DIDTSVQLQALAGGYDWFGYGSKVIVTTKNEQLLDIHGFNKLRSVPELNYDEALELFSWH 360
            DIDTS QLQ LAGGYDWFG+GSKVIVTT+NEQLLDIHGF KL+ VP+L++ EALELFSWH
Sbjct: 301  DIDTSEQLQVLAGGYDWFGHGSKVIVTTRNEQLLDIHGFYKLKEVPQLHFGEALELFSWH 360

Query: 361  AFQSSRPPSEYLQLSIDAVNYCKNLPLALEVLGSFLYSTDQSKFKGILEEFAISNLDKDI 420
            AF +S PPSEY  L  DAVNYCKNLPLALEVLGSFLYSTDQSKFKGILEEFA SNL+KDI
Sbjct: 361  AFHNSCPPSEYSTLPEDAVNYCKNLPLALEVLGSFLYSTDQSKFKGILEEFANSNLNKDI 420

Query: 421  QNLLQVSYDELEGDVQEMFLFISCFFVGEDKTMVETMLKSCGCLCWERGIKKLMNLSLFT 480
            Q LLQVSYDELEGDVQEMFLFISCFFVGEDKTMVE MLKSCGCLCWE GIKKLMNLSL T
Sbjct: 421  QKLLQVSYDELEGDVQEMFLFISCFFVGEDKTMVEMMLKSCGCLCWENGIKKLMNLSLLT 480

Query: 481  INKQNEVEMHDLIQQMGHTIARSKTSICPSEKKLLVGDEAMHVLDGIKEARAVKAIKLEF 540
            IN+ N+V+MHDLI+QMGHTIARS+T I  SEKK++V DEAMHVLDGI EARAVKAIK+EF
Sbjct: 481  INRINKVQMHDLIKQMGHTIARSRTFISHSEKKIMVEDEAMHVLDGINEARAVKAIKMEF 540

Query: 541  PKPTKLDIIDSTAFRKVKNLVVLKVKNVRSSKISTLDFLPNSLRWMSWSEFPFSSFPSSY 600
            P PT+LDIIDS AF KVKNL VLKVKNV  SKISTLD LPNSLRWMSWS FPFSSFPSSY
Sbjct: 541  PNPTELDIIDSNAFSKVKNLAVLKVKNVTFSKISTLDSLPNSLRWMSWSGFPFSSFPSSY 600

Query: 601  SMENLIQLKLPHSSIEHFGR-AFMHCERLKQLDLSNSFFLEEIPDLSAAINLENLSLSGC 660
            SMEN+IQLKLPHSSI+ F + AF HC+ LK+LDLSNS FLEEIPDLSAA NLE LSLSGC
Sbjct: 601  SMENIIQLKLPHSSIKRFEKEAFTHCKWLKELDLSNSIFLEEIPDLSAATNLEKLSLSGC 660

Query: 661  IGLVKVHKSVGSLGKLVDLSLSSHVYGFKQFPSPLKLKSLKRFSTDHCTIVQGYPQFSEE 720
              LVKVHKSVGSLGKLVDL +SSHVYGF+QFPSPLKLKSLKRFST HCTIV+GYPQFS+E
Sbjct: 661  ENLVKVHKSVGSLGKLVDLCISSHVYGFEQFPSPLKLKSLKRFSTYHCTIVRGYPQFSKE 720

Query: 721  MKSSLEDLWFYSSSITELSSTIRYLTSLKDLTITDCKKLTTLPSTIYDLSKLTSIEVSQS 780
            M+SSLE LWFY SSITELSSTIRYLTSLK L+ITDCK+LTTLPSTIYDLSKLTSIEVSQS
Sbjct: 721  MESSLEHLWFYRSSITELSSTIRYLTSLKILSITDCKELTTLPSTIYDLSKLTSIEVSQS 780

Query: 781  DFSTFPSSYSCPSSLTHLTRLHLYENKITNLDFLETIAHVAPSLRELNL----------- 840
            D STFP SYSCPSSL HLTRL LYENKITNLDFLETIAH APSLRELNL           
Sbjct: 781  DLSTFPFSYSCPSSLLHLTRLDLYENKITNLDFLETIAHAAPSLRELNLSNNNFSILPSC 840

Query: 841  --------FLETFDCKLLEEIPKIPEGLISLGAEGCISLAKFPDNLADFISCDSEHVDGQ 900
                    FLET DCKLL++IPKIPEGLI L A+GCISLAKFPDNLADFISCDSEHVDGQ
Sbjct: 841  IVNFKSLRFLETIDCKLLKKIPKIPEGLIYLDAQGCISLAKFPDNLADFISCDSEHVDGQ 900

Query: 901  FKQLILMNCDIPDWFSYKSRNNPMTIFMPSNDPRWEFKVFAPCVKFQVNHVDRNRYMDLE 960
            FKQLILMNCDIPDWFSYKSRNNP+T+ +PSNDP  E KVFA CVKFQVNHVD+++YMDLE
Sbjct: 901  FKQLILMNCDIPDWFSYKSRNNPITLLVPSNDPSSELKVFAACVKFQVNHVDQDQYMDLE 960

Query: 961  CKVLINDIQVWSSEEVPFLEESRRILIEASPYEYMWLLVLYPHIDFPLNWDDIIKRSQEF 1020
            CKV INDIQVWS EEVPF +ESR ILI+ASP+EYMWLLVLYPHI+F LN DDII RSQE 
Sbjct: 961  CKVFINDIQVWSYEEVPFHDESRSILIKASPHEYMWLLVLYPHINFRLNSDDIINRSQEI 1020

Query: 1021 NLHQPSFGINSMGRDYNNCDVDDD-RRNHFGESIWRKFTVSFGVNSKFKDSEVSIKRCGV 1068
            NLHQPSFGINS+GRD NNC+VDDD RRNH GESIWRKFTVSFGV SKFKDSE+SIK CGV
Sbjct: 1021 NLHQPSFGINSIGRDNNNCNVDDDYRRNHIGESIWRKFTVSFGVTSKFKDSELSIKTCGV 1080

BLAST of PI0000332 vs. ExPASy TrEMBL
Match: A0A5A7V625 (TMV resistance protein N-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold329G002110 PE=4 SV=1)

HSP 1 Score: 1607.0 bits (4160), Expect = 0.0e+00
Identity = 823/903 (91.14%), Postives = 844/903 (93.47%), Query Frame = 0

Query: 1   MDRASGSFSSHFRCSFDVFLSFRGEDTRFNFTSHLDTALRQRGINVFIDNKLTRGANFPP 60
           MDR+SGSFSSHFR SFDVFLSFRGEDTRFNFTSHL TALRQRGINVFIDNKLTRG  FPP
Sbjct: 1   MDRSSGSFSSHFRWSFDVFLSFRGEDTRFNFTSHLYTALRQRGINVFIDNKLTRGDKFPP 60

Query: 61  SLLKAIEESKISVVIISENYASSSWCLNELVHIIMGNELRGQVVLPIFYKVNPSQVRTQN 120
           SLLKAIEESKIS+VIISENYASSSWCLNELVHI M NELRGQVVLP+FYKVNPSQVR Q+
Sbjct: 61  SLLKAIEESKISIVIISENYASSSWCLNELVHINMCNELRGQVVLPVFYKVNPSQVRKQD 120

Query: 121 GAFGEAFAELEVRFFDKMQAWRDALTAISYMSGWVVLQKDDEANLIQKIVQQVWKKLTGS 180
               EAFAELEVRF DKMQAWR+ALTA+SYMSGWVVLQ DDEANLIQKIVQQVWKKLT S
Sbjct: 121 ----EAFAELEVRFSDKMQAWREALTAVSYMSGWVVLQNDDEANLIQKIVQQVWKKLTCS 180

Query: 181 TMQLPVTKYPVGIDRQVENLLSHVMIDGTRMVGLYGIGGMGKTTLAKALYNRIADDFEGC 240
           TMQLPVTKYPVGIDRQVENLLSHVMIDGTRMVG+YGIGGMGKTTLAK LYNRIADDFEG 
Sbjct: 181 TMQLPVTKYPVGIDRQVENLLSHVMIDGTRMVGVYGIGGMGKTTLAKTLYNRIADDFEGY 240

Query: 241 CFLANIREASKEHEGLVRLQEKLLYEILMDDFIRVGNLHRGINIIRNRLCSKKILLILDD 300
           CFLAN+REASK+HEGLV+LQEKLLYEILMDDFIRVG+L+RGINIIRNRLCSKKILLILDD
Sbjct: 241 CFLANVREASKQHEGLVKLQEKLLYEILMDDFIRVGDLYRGINIIRNRLCSKKILLILDD 300

Query: 301 IDTSVQLQALAGGYDWFGYGSKVIVTTKNEQLLDIHGFNKLRSVPELNYDEALELFSWHA 360
           IDTS QLQALAGGYDWFGYGSKVIVTT+NEQLLDIHGFNKLRSVPELNYDEALELFSWHA
Sbjct: 301 IDTSEQLQALAGGYDWFGYGSKVIVTTRNEQLLDIHGFNKLRSVPELNYDEALELFSWHA 360

Query: 361 FQSSRPPSEYLQLSIDAVNYCKNLPLALEVLGSFLYSTDQSKFKGILEEFAISNLDKDIQ 420
           FQ S PPSEYLQLS DAVNYCKNLPLALEVLGSFLYSTDQSKF+GILEEFAISNLDKDIQ
Sbjct: 361 FQCSHPPSEYLQLSKDAVNYCKNLPLALEVLGSFLYSTDQSKFRGILEEFAISNLDKDIQ 420

Query: 421 NLLQVSYDELEGDVQEMFLFISCFFVGEDKTMVETMLKSCGCLCWERGIKKLMNLSLFTI 480
           NLLQVSYDELEGDVQEMFLFISCFFVGEDKTMVETMLKSCGCLCWE+GIKKLMNLSL TI
Sbjct: 421 NLLQVSYDELEGDVQEMFLFISCFFVGEDKTMVETMLKSCGCLCWEKGIKKLMNLSLLTI 480

Query: 481 NKQNEVEMHDLIQQMGHTIARSKTSICPSEKKLLVGDEAMHVLDGIKEARAVKAIKLEFP 540
           NK N+VEMHDLIQQMGHTIARSKTSI  SEKKLLVGD+AMHV DGIKEARAVKAIKLEFP
Sbjct: 481 NKWNKVEMHDLIQQMGHTIARSKTSISASEKKLLVGDDAMHVFDGIKEARAVKAIKLEFP 540

Query: 541 KPTKLDIIDSTAFRKVKNLVVLKVKNVRSSKISTLDFLPNSLRWMSWSEFPFSSFPSSYS 600
           KPTKLDIIDS+AFRKVKNLVVLKVKNV SSKISTL+FLPNSLRWMSWSEFPFSSFPSSYS
Sbjct: 541 KPTKLDIIDSSAFRKVKNLVVLKVKNVISSKISTLEFLPNSLRWMSWSEFPFSSFPSSYS 600

Query: 601 MENLIQLKLPHSSIEHFGRAFMHCERLKQLDLSNSFFLEEIPDLSAAINLENLSLSGCIG 660
           MENLIQLKLPHSSIEHFGRAFM CE LKQLDLSNSFFLEEIPDLSAAINLENLSLSGCI 
Sbjct: 601 MENLIQLKLPHSSIEHFGRAFMRCESLKQLDLSNSFFLEEIPDLSAAINLENLSLSGCIS 660

Query: 661 LVKVHKSVGSLGKLVDLSLSSHVYGFKQFPSPLKLKSLKRFSTDHCTIVQGYPQFSEEMK 720
           LVKVHKSVGSLGKLVDLSLSSHVYGFKQFPSPLKLKSLKRFSTDHCTIVQGYPQFS+EMK
Sbjct: 661 LVKVHKSVGSLGKLVDLSLSSHVYGFKQFPSPLKLKSLKRFSTDHCTIVQGYPQFSQEMK 720

Query: 721 SSLEDLWFYSSSITELSSTIRYLTSLKDLTITDCKKLTTLPSTIYDLSKLTSIEVSQSDF 780
           SSLEDLWFYSSSITELSSTIRYLTSLKDLTITDCKKLTTLPSTIYDLSKLTSIEVSQSD 
Sbjct: 721 SSLEDLWFYSSSITELSSTIRYLTSLKDLTITDCKKLTTLPSTIYDLSKLTSIEVSQSDL 780

Query: 781 STFPSSYSCPSSLTHLTRLHLYENKITNLDFLETIAHVAPSLRELNL------------- 840
           STFPSSYSCPSSL HLTRLHLYENKITNLDFLETIAH APSLRELNL             
Sbjct: 781 STFPSSYSCPSSLPHLTRLHLYENKITNLDFLETIAHAAPSLRELNLSNNNFSILPSCIV 840

Query: 841 ------FLETFDCKLLEEIPKIPEGLISLGAEGCISLAKFPDNLADFISCDSEHVDGQFK 885
                 FLETFDCKLLEEIPKIPEGLISLGAEGCISLAKFPDNLADFIS DS H+     
Sbjct: 841 NFKSLRFLETFDCKLLEEIPKIPEGLISLGAEGCISLAKFPDNLADFISSDSVHISSPSL 899

BLAST of PI0000332 vs. ExPASy TrEMBL
Match: A0A1S3C131 (TMV resistance protein N-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103495278 PE=4 SV=1)

HSP 1 Score: 1567.4 bits (4057), Expect = 0.0e+00
Identity = 818/1089 (75.11%), Postives = 884/1089 (81.18%), Query Frame = 0

Query: 1    MDRASGSFSSHFRCSFDVFLSFRGEDTRFNFTSHLDTALRQRGINVFI-DNKLTRGANFP 60
            MDRASGSFSSH R  FDVFLSFRGEDTRFNFTSHL TALRQRGINVFI D++LTRG NFP
Sbjct: 1    MDRASGSFSSH-RWRFDVFLSFRGEDTRFNFTSHLYTALRQRGINVFIDDSELTRGENFP 60

Query: 61   PSLLKAIEESKISVVIISENYASSSWCLNELVHIIMGNELRGQVVLPIFYKVNPSQVRTQ 120
             SLL+AIEESKISVVIISENYA+SSWCLNELV++IM  +LRGQVVLPIFYKVNPSQVRTQ
Sbjct: 61   SSLLRAIEESKISVVIISENYATSSWCLNELVYLIMCKKLRGQVVLPIFYKVNPSQVRTQ 120

Query: 121  NGAFGEAFAELEVRFFDKMQAWRDALTAISYMSGWVVLQKDDEANLIQKIVQQVWKKLTG 180
            NGAFGEAFA+LEVRFFDKMQAWR+ALT +S+MSGWV+LQ DDEA LIQ IV+ VWKKLT 
Sbjct: 121  NGAFGEAFAKLEVRFFDKMQAWREALTTVSFMSGWVLLQNDDEARLIQIIVRHVWKKLTC 180

Query: 181  STMQLPVTKYPVGIDRQVENLLSHVMIDGTRMVGLYGIGGMGKTTLAKALYNRIADDFEG 240
            ST+QL VTKYPVGIDRQV++LLSHV+ID TRMVGLYGIGGMGKTTLAKALYNR+AD FEG
Sbjct: 181  STVQLSVTKYPVGIDRQVKDLLSHVIIDETRMVGLYGIGGMGKTTLAKALYNRVADKFEG 240

Query: 241  CCFLANIREASKEHEGLVRLQEKLLYEILMDDFIRVGNLHRGINIIRNRLCSKKILLILD 300
            CCFLANIREASK+H+GLVRLQEKLLY+ILM DF+RVG++++GINIIRNRL S +ILLILD
Sbjct: 241  CCFLANIREASKQHDGLVRLQEKLLYDILMYDFVRVGDVYKGINIIRNRLYSIRILLILD 300

Query: 301  DIDTSVQLQALAGGYDWFGYGSKVIVTTKNEQLLDIHGFNKLRSVPELNYDEALELFSWH 360
            DIDTS QLQ LAGGYDWFG+GSKVIVTT+NEQLLDIHGF KL+ VP+L++ EALELFSWH
Sbjct: 301  DIDTSEQLQVLAGGYDWFGHGSKVIVTTRNEQLLDIHGFYKLKEVPQLHFGEALELFSWH 360

Query: 361  AFQSSRPPSEYLQLSIDAVNYCKNLPLALEVLGSFLYSTDQSKFKGILEEFAISNLDKDI 420
            AF +S PPSEY  L  DAVNYCKNLPLALEVLGSFLYSTDQSKFKGILEEFA SNL+KDI
Sbjct: 361  AFHNSCPPSEYSTLPEDAVNYCKNLPLALEVLGSFLYSTDQSKFKGILEEFANSNLNKDI 420

Query: 421  QNLLQVSYDELEGDVQEMFLFISCFFVGEDKTMVETMLKSCGCLCWERGIKKLMNLSLFT 480
            Q LLQ                                                       
Sbjct: 421  QKLLQ------------------------------------------------------- 480

Query: 481  INKQNEVEMHDLIQQMGHTIARSKTSICPSEKKLLVGDEAMHVLDGIKEARAVKAIKLEF 540
                                                            EARAVKAIK+EF
Sbjct: 481  ------------------------------------------------EARAVKAIKMEF 540

Query: 541  PKPTKLDIIDSTAFRKVKNLVVLKVKNVRSSKISTLDFLPNSLRWMSWSEFPFSSFPSSY 600
            P PT+LDIIDS AF KVKNL VLKVKNV  SKISTLD LPNSLRWMSWS FPFSSFPSSY
Sbjct: 541  PNPTELDIIDSNAFSKVKNLAVLKVKNVTFSKISTLDSLPNSLRWMSWSGFPFSSFPSSY 600

Query: 601  SMENLIQLKLPHSSIEHFGR-AFMHCERLKQLDLSNSFFLEEIPDLSAAINLENLSLSGC 660
            SMEN+IQLKLPHSSI+ F + AF HC+ LK+LDLSNS FLEEIPDLSAA NLE LSLSGC
Sbjct: 601  SMENIIQLKLPHSSIKRFEKEAFTHCKWLKELDLSNSIFLEEIPDLSAATNLEKLSLSGC 660

Query: 661  IGLVKVHKSVGSLGKLVDLSLSSHVYGFKQFPSPLKLKSLKRFSTDHCTIVQGYPQFSEE 720
              LVKVHKSVGSLGKLVDL +SSHVYGF+QFPSPLKLKSLKRFST HCTIV+GYPQFS+E
Sbjct: 661  ENLVKVHKSVGSLGKLVDLCISSHVYGFEQFPSPLKLKSLKRFSTYHCTIVRGYPQFSKE 720

Query: 721  MKSSLEDLWFYSSSITELSSTIRYLTSLKDLTITDCKKLTTLPSTIYDLSKLTSIEVSQS 780
            M+SSLE LWFY SSITELSSTIRYLTSLK L+ITDCK+LTTLPSTIYDLSKLTSIEVSQS
Sbjct: 721  MESSLEHLWFYRSSITELSSTIRYLTSLKILSITDCKELTTLPSTIYDLSKLTSIEVSQS 780

Query: 781  DFSTFPSSYSCPSSLTHLTRLHLYENKITNLDFLETIAHVAPSLRELNL----------- 840
            D STFP SYSCPSSL HLTRL LYENKITNLDFLETIAH APSLRELNL           
Sbjct: 781  DLSTFPFSYSCPSSLLHLTRLDLYENKITNLDFLETIAHAAPSLRELNLSNNNFSILPSC 840

Query: 841  --------FLETFDCKLLEEIPKIPEGLISLGAEGCISLAKFPDNLADFISCDSEHVDGQ 900
                    FLET DCKLL++IPKIPEGLI L A+GCISLAKFPDNLADFISCDSEHVDGQ
Sbjct: 841  IVNFKSLRFLETIDCKLLKKIPKIPEGLIYLDAQGCISLAKFPDNLADFISCDSEHVDGQ 900

Query: 901  FKQLILMNCDIPDWFSYKSRNNPMTIFMPSNDPRWEFKVFAPCVKFQVNHVDRNRYMDLE 960
            FKQLILMNCDIPDWFSYKSRNNP+T+ +PSNDP  E KVFA CVKFQVNHVD+++YMDLE
Sbjct: 901  FKQLILMNCDIPDWFSYKSRNNPITLLVPSNDPSSELKVFAACVKFQVNHVDQDQYMDLE 960

Query: 961  CKVLINDIQVWSSEEVPFLEESRRILIEASPYEYMWLLVLYPHIDFPLNWDDIIKRSQEF 1020
            CKV INDIQVWS EEVPF +ESR ILI+ASP+EYMWLLVLYPHI+F LN DDII RSQE 
Sbjct: 961  CKVFINDIQVWSYEEVPFHDESRSILIKASPHEYMWLLVLYPHINFRLNSDDIINRSQEI 985

Query: 1021 NLHQPSFGINSMGRDYNNCDVDDD-RRNHFGESIWRKFTVSFGVNSKFKDSEVSIKRCGV 1068
            NLHQPSFGINS+GRD NNC+VDDD RRNH GESIWRKFTVSFGV SKFKDSE+SIK CGV
Sbjct: 1021 NLHQPSFGINSIGRDNNNCNVDDDYRRNHIGESIWRKFTVSFGVTSKFKDSELSIKTCGV 985

BLAST of PI0000332 vs. ExPASy TrEMBL
Match: A0A1S4DZX2 (TMV resistance protein N-like isoform X3 OS=Cucumis melo OX=3656 GN=LOC103495278 PE=4 SV=1)

HSP 1 Score: 1506.5 bits (3899), Expect = 0.0e+00
Identity = 771/923 (83.53%), Postives = 830/923 (89.92%), Query Frame = 0

Query: 1   MDRASGSFSSHFRCSFDVFLSFRGEDTRFNFTSHLDTALRQRGINVFI-DNKLTRGANFP 60
           MDRASGSFSSH R  FDVFLSFRGEDTRFNFTSHL TALRQRGINVFI D++LTRG NFP
Sbjct: 1   MDRASGSFSSH-RWRFDVFLSFRGEDTRFNFTSHLYTALRQRGINVFIDDSELTRGENFP 60

Query: 61  PSLLKAIEESKISVVIISENYASSSWCLNELVHIIMGNELRGQVVLPIFYKVNPSQVRTQ 120
            SLL+AIEESKISVVIISENYA+SSWCLNELV++IM  +LRGQVVLPIFYKVNPSQVRTQ
Sbjct: 61  SSLLRAIEESKISVVIISENYATSSWCLNELVYLIMCKKLRGQVVLPIFYKVNPSQVRTQ 120

Query: 121 NGAFGEAFAELEVRFFDKMQAWRDALTAISYMSGWVVLQKDDEANLIQKIVQQVWKKLTG 180
           NGAFGEAFA+LEVRFFDKMQAWR+ALT +S+MSGWV+LQ DDEA LIQ IV+ VWKKLT 
Sbjct: 121 NGAFGEAFAKLEVRFFDKMQAWREALTTVSFMSGWVLLQNDDEARLIQIIVRHVWKKLTC 180

Query: 181 STMQLPVTKYPVGIDRQVENLLSHVMIDGTRMVGLYGIGGMGKTTLAKALYNRIADDFEG 240
           ST+QL VTKYPVGIDRQV++LLSHV+ID TRMVGLYGIGGMGKTTLAKALYNR+AD FEG
Sbjct: 181 STVQLSVTKYPVGIDRQVKDLLSHVIIDETRMVGLYGIGGMGKTTLAKALYNRVADKFEG 240

Query: 241 CCFLANIREASKEHEGLVRLQEKLLYEILMDDFIRVGNLHRGINIIRNRLCSKKILLILD 300
           CCFLANIREASK+H+GLVRLQEKLLY+ILM DF+RVG++++GINIIRNRL S +ILLILD
Sbjct: 241 CCFLANIREASKQHDGLVRLQEKLLYDILMYDFVRVGDVYKGINIIRNRLYSIRILLILD 300

Query: 301 DIDTSVQLQALAGGYDWFGYGSKVIVTTKNEQLLDIHGFNKLRSVPELNYDEALELFSWH 360
           DIDTS QLQ LAGGYDWFG+GSKVIVTT+NEQLLDIHGF KL+ VP+L++ EALELFSWH
Sbjct: 301 DIDTSEQLQVLAGGYDWFGHGSKVIVTTRNEQLLDIHGFYKLKEVPQLHFGEALELFSWH 360

Query: 361 AFQSSRPPSEYLQLSIDAVNYCKNLPLALEVLGSFLYSTDQSKFKGILEEFAISNLDKDI 420
           AF +S PPSEY  L  DAVNYCKNLPLALEVLGSFLYSTDQSKFKGILEEFA SNL+KDI
Sbjct: 361 AFHNSCPPSEYSTLPEDAVNYCKNLPLALEVLGSFLYSTDQSKFKGILEEFANSNLNKDI 420

Query: 421 QNLLQVSYDELEGDVQEMFLFISCFFVGEDKTMVETMLKSCGCLCWERGIKKLMNLSLFT 480
           Q LLQVSYDELEGDVQEMFLFISCFFVGEDKTMVE MLKSCGCLCWE GIKKLMNLSL T
Sbjct: 421 QKLLQVSYDELEGDVQEMFLFISCFFVGEDKTMVEMMLKSCGCLCWENGIKKLMNLSLLT 480

Query: 481 INKQNEVEMHDLIQQMGHTIARSKTSICPSEKKLLVGDEAMHVLDGIKEARAVKAIKLEF 540
           IN+ N+V+MHDLI+QMGHTIARS+T I  SEKK++V DEAMHVLDGI EARAVKAIK+EF
Sbjct: 481 INRINKVQMHDLIKQMGHTIARSRTFISHSEKKIMVEDEAMHVLDGINEARAVKAIKMEF 540

Query: 541 PKPTKLDIIDSTAFRKVKNLVVLKVKNVRSSKISTLDFLPNSLRWMSWSEFPFSSFPSSY 600
           P PT+LDIIDS AF KVKNL VLKVKNV  SKISTLD LPNSLRWMSWS FPFSSFPSSY
Sbjct: 541 PNPTELDIIDSNAFSKVKNLAVLKVKNVTFSKISTLDSLPNSLRWMSWSGFPFSSFPSSY 600

Query: 601 SMENLIQLKLPHSSIEHFGR-AFMHCERLKQLDLSNSFFLEEIPDLSAAINLENLSLSGC 660
           SMEN+IQLKLPHSSI+ F + AF HC+ LK+LDLSNS FLEEIPDLSAA NLE LSLSGC
Sbjct: 601 SMENIIQLKLPHSSIKRFEKEAFTHCKWLKELDLSNSIFLEEIPDLSAATNLEKLSLSGC 660

Query: 661 IGLVKVHKSVGSLGKLVDLSLSSHVYGFKQFPSPLKLKSLKRFSTDHCTIVQGYPQFSEE 720
             LVKVHKSVGSLGKLVDL +SSHVYGF+QFPSPLKLKSLKRFST HCTIV+GYPQFS+E
Sbjct: 661 ENLVKVHKSVGSLGKLVDLCISSHVYGFEQFPSPLKLKSLKRFSTYHCTIVRGYPQFSKE 720

Query: 721 MKSSLEDLWFYSSSITELSSTIRYLTSLKDLTITDCKKLTTLPSTIYDLSKLTSIEVSQS 780
           M+SSLE LWFY SSITELSSTIRYLTSLK L+ITDCK+LTTLPSTIYDLSKLTSIEVSQS
Sbjct: 721 MESSLEHLWFYRSSITELSSTIRYLTSLKILSITDCKELTTLPSTIYDLSKLTSIEVSQS 780

Query: 781 DFSTFPSSYSCPSSLTHLTRLHLYENKITNLDFLETIAHVAPSLRELNL----------- 840
           D STFP SYSCPSSL HLTRL LYENKITNLDFLETIAH APSLRELNL           
Sbjct: 781 DLSTFPFSYSCPSSLLHLTRLDLYENKITNLDFLETIAHAAPSLRELNLSNNNFSILPSC 840

Query: 841 --------FLETFDCKLLEEIPKIPEGLISLGAEGCISLAKFPDNLADFISCDSEHVDGQ 900
                   FLET DCKLL++IPKIPEGLI L A+GCISLAKFPDNLADFISCDSEHVDGQ
Sbjct: 841 IVNFKSLRFLETIDCKLLKKIPKIPEGLIYLDAQGCISLAKFPDNLADFISCDSEHVDGQ 900

Query: 901 FKQLILMNCDIPDWFSYKSRNNP 903
           FKQLILMNCDIPDWFSYKSRNNP
Sbjct: 901 FKQLILMNCDIPDWFSYKSRNNP 922

BLAST of PI0000332 vs. NCBI nr
Match: XP_016901532.1 (PREDICTED: TMV resistance protein N-like isoform X1 [Cucumis melo])

HSP 1 Score: 1786.9 bits (4627), Expect = 0.0e+00
Identity = 905/1089 (83.10%), Postives = 978/1089 (89.81%), Query Frame = 0

Query: 1    MDRASGSFSSHFRCSFDVFLSFRGEDTRFNFTSHLDTALRQRGINVFI-DNKLTRGANFP 60
            MDRASGSFSSH R  FDVFLSFRGEDTRFNFTSHL TALRQRGINVFI D++LTRG NFP
Sbjct: 1    MDRASGSFSSH-RWRFDVFLSFRGEDTRFNFTSHLYTALRQRGINVFIDDSELTRGENFP 60

Query: 61   PSLLKAIEESKISVVIISENYASSSWCLNELVHIIMGNELRGQVVLPIFYKVNPSQVRTQ 120
             SLL+AIEESKISVVIISENYA+SSWCLNELV++IM  +LRGQVVLPIFYKVNPSQVRTQ
Sbjct: 61   SSLLRAIEESKISVVIISENYATSSWCLNELVYLIMCKKLRGQVVLPIFYKVNPSQVRTQ 120

Query: 121  NGAFGEAFAELEVRFFDKMQAWRDALTAISYMSGWVVLQKDDEANLIQKIVQQVWKKLTG 180
            NGAFGEAFA+LEVRFFDKMQAWR+ALT +S+MSGWV+LQ DDEA LIQ IV+ VWKKLT 
Sbjct: 121  NGAFGEAFAKLEVRFFDKMQAWREALTTVSFMSGWVLLQNDDEARLIQIIVRHVWKKLTC 180

Query: 181  STMQLPVTKYPVGIDRQVENLLSHVMIDGTRMVGLYGIGGMGKTTLAKALYNRIADDFEG 240
            ST+QL VTKYPVGIDRQV++LLSHV+ID TRMVGLYGIGGMGKTTLAKALYNR+AD FEG
Sbjct: 181  STVQLSVTKYPVGIDRQVKDLLSHVIIDETRMVGLYGIGGMGKTTLAKALYNRVADKFEG 240

Query: 241  CCFLANIREASKEHEGLVRLQEKLLYEILMDDFIRVGNLHRGINIIRNRLCSKKILLILD 300
            CCFLANIREASK+H+GLVRLQEKLLY+ILM DF+RVG++++GINIIRNRL S +ILLILD
Sbjct: 241  CCFLANIREASKQHDGLVRLQEKLLYDILMYDFVRVGDVYKGINIIRNRLYSIRILLILD 300

Query: 301  DIDTSVQLQALAGGYDWFGYGSKVIVTTKNEQLLDIHGFNKLRSVPELNYDEALELFSWH 360
            DIDTS QLQ LAGGYDWFG+GSKVIVTT+NEQLLDIHGF KL+ VP+L++ EALELFSWH
Sbjct: 301  DIDTSEQLQVLAGGYDWFGHGSKVIVTTRNEQLLDIHGFYKLKEVPQLHFGEALELFSWH 360

Query: 361  AFQSSRPPSEYLQLSIDAVNYCKNLPLALEVLGSFLYSTDQSKFKGILEEFAISNLDKDI 420
            AF +S PPSEY  L  DAVNYCKNLPLALEVLGSFLYSTDQSKFKGILEEFA SNL+KDI
Sbjct: 361  AFHNSCPPSEYSTLPEDAVNYCKNLPLALEVLGSFLYSTDQSKFKGILEEFANSNLNKDI 420

Query: 421  QNLLQVSYDELEGDVQEMFLFISCFFVGEDKTMVETMLKSCGCLCWERGIKKLMNLSLFT 480
            Q LLQVSYDELEGDVQEMFLFISCFFVGEDKTMVE MLKSCGCLCWE GIKKLMNLSL T
Sbjct: 421  QKLLQVSYDELEGDVQEMFLFISCFFVGEDKTMVEMMLKSCGCLCWENGIKKLMNLSLLT 480

Query: 481  INKQNEVEMHDLIQQMGHTIARSKTSICPSEKKLLVGDEAMHVLDGIKEARAVKAIKLEF 540
            IN+ N+V+MHDLI+QMGHTIARS+T I  SEKK++V DEAMHVLDGI EARAVKAIK+EF
Sbjct: 481  INRINKVQMHDLIKQMGHTIARSRTFISHSEKKIMVEDEAMHVLDGINEARAVKAIKMEF 540

Query: 541  PKPTKLDIIDSTAFRKVKNLVVLKVKNVRSSKISTLDFLPNSLRWMSWSEFPFSSFPSSY 600
            P PT+LDIIDS AF KVKNL VLKVKNV  SKISTLD LPNSLRWMSWS FPFSSFPSSY
Sbjct: 541  PNPTELDIIDSNAFSKVKNLAVLKVKNVTFSKISTLDSLPNSLRWMSWSGFPFSSFPSSY 600

Query: 601  SMENLIQLKLPHSSIEHFGR-AFMHCERLKQLDLSNSFFLEEIPDLSAAINLENLSLSGC 660
            SMEN+IQLKLPHSSI+ F + AF HC+ LK+LDLSNS FLEEIPDLSAA NLE LSLSGC
Sbjct: 601  SMENIIQLKLPHSSIKRFEKEAFTHCKWLKELDLSNSIFLEEIPDLSAATNLEKLSLSGC 660

Query: 661  IGLVKVHKSVGSLGKLVDLSLSSHVYGFKQFPSPLKLKSLKRFSTDHCTIVQGYPQFSEE 720
              LVKVHKSVGSLGKLVDL +SSHVYGF+QFPSPLKLKSLKRFST HCTIV+GYPQFS+E
Sbjct: 661  ENLVKVHKSVGSLGKLVDLCISSHVYGFEQFPSPLKLKSLKRFSTYHCTIVRGYPQFSKE 720

Query: 721  MKSSLEDLWFYSSSITELSSTIRYLTSLKDLTITDCKKLTTLPSTIYDLSKLTSIEVSQS 780
            M+SSLE LWFY SSITELSSTIRYLTSLK L+ITDCK+LTTLPSTIYDLSKLTSIEVSQS
Sbjct: 721  MESSLEHLWFYRSSITELSSTIRYLTSLKILSITDCKELTTLPSTIYDLSKLTSIEVSQS 780

Query: 781  DFSTFPSSYSCPSSLTHLTRLHLYENKITNLDFLETIAHVAPSLRELNL----------- 840
            D STFP SYSCPSSL HLTRL LYENKITNLDFLETIAH APSLRELNL           
Sbjct: 781  DLSTFPFSYSCPSSLLHLTRLDLYENKITNLDFLETIAHAAPSLRELNLSNNNFSILPSC 840

Query: 841  --------FLETFDCKLLEEIPKIPEGLISLGAEGCISLAKFPDNLADFISCDSEHVDGQ 900
                    FLET DCKLL++IPKIPEGLI L A+GCISLAKFPDNLADFISCDSEHVDGQ
Sbjct: 841  IVNFKSLRFLETIDCKLLKKIPKIPEGLIYLDAQGCISLAKFPDNLADFISCDSEHVDGQ 900

Query: 901  FKQLILMNCDIPDWFSYKSRNNPMTIFMPSNDPRWEFKVFAPCVKFQVNHVDRNRYMDLE 960
            FKQLILMNCDIPDWFSYKSRNNP+T+ +PSNDP  E KVFA CVKFQVNHVD+++YMDLE
Sbjct: 901  FKQLILMNCDIPDWFSYKSRNNPITLLVPSNDPSSELKVFAACVKFQVNHVDQDQYMDLE 960

Query: 961  CKVLINDIQVWSSEEVPFLEESRRILIEASPYEYMWLLVLYPHIDFPLNWDDIIKRSQEF 1020
            CKV INDIQVWS EEVPF +ESR ILI+ASP+EYMWLLVLYPHI+F LN DDII RSQE 
Sbjct: 961  CKVFINDIQVWSYEEVPFHDESRSILIKASPHEYMWLLVLYPHINFRLNSDDIINRSQEI 1020

Query: 1021 NLHQPSFGINSMGRDYNNCDVDDD-RRNHFGESIWRKFTVSFGVNSKFKDSEVSIKRCGV 1068
            NLHQPSFGINS+GRD NNC+VDDD RRNH GESIWRKFTVSFGV SKFKDSE+SIK CGV
Sbjct: 1021 NLHQPSFGINSIGRDNNNCNVDDDYRRNHIGESIWRKFTVSFGVTSKFKDSELSIKTCGV 1080

BLAST of PI0000332 vs. NCBI nr
Match: KAA0063692.1 (TMV resistance protein N-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1607.0 bits (4160), Expect = 0.0e+00
Identity = 823/903 (91.14%), Postives = 844/903 (93.47%), Query Frame = 0

Query: 1   MDRASGSFSSHFRCSFDVFLSFRGEDTRFNFTSHLDTALRQRGINVFIDNKLTRGANFPP 60
           MDR+SGSFSSHFR SFDVFLSFRGEDTRFNFTSHL TALRQRGINVFIDNKLTRG  FPP
Sbjct: 1   MDRSSGSFSSHFRWSFDVFLSFRGEDTRFNFTSHLYTALRQRGINVFIDNKLTRGDKFPP 60

Query: 61  SLLKAIEESKISVVIISENYASSSWCLNELVHIIMGNELRGQVVLPIFYKVNPSQVRTQN 120
           SLLKAIEESKIS+VIISENYASSSWCLNELVHI M NELRGQVVLP+FYKVNPSQVR Q+
Sbjct: 61  SLLKAIEESKISIVIISENYASSSWCLNELVHINMCNELRGQVVLPVFYKVNPSQVRKQD 120

Query: 121 GAFGEAFAELEVRFFDKMQAWRDALTAISYMSGWVVLQKDDEANLIQKIVQQVWKKLTGS 180
               EAFAELEVRF DKMQAWR+ALTA+SYMSGWVVLQ DDEANLIQKIVQQVWKKLT S
Sbjct: 121 ----EAFAELEVRFSDKMQAWREALTAVSYMSGWVVLQNDDEANLIQKIVQQVWKKLTCS 180

Query: 181 TMQLPVTKYPVGIDRQVENLLSHVMIDGTRMVGLYGIGGMGKTTLAKALYNRIADDFEGC 240
           TMQLPVTKYPVGIDRQVENLLSHVMIDGTRMVG+YGIGGMGKTTLAK LYNRIADDFEG 
Sbjct: 181 TMQLPVTKYPVGIDRQVENLLSHVMIDGTRMVGVYGIGGMGKTTLAKTLYNRIADDFEGY 240

Query: 241 CFLANIREASKEHEGLVRLQEKLLYEILMDDFIRVGNLHRGINIIRNRLCSKKILLILDD 300
           CFLAN+REASK+HEGLV+LQEKLLYEILMDDFIRVG+L+RGINIIRNRLCSKKILLILDD
Sbjct: 241 CFLANVREASKQHEGLVKLQEKLLYEILMDDFIRVGDLYRGINIIRNRLCSKKILLILDD 300

Query: 301 IDTSVQLQALAGGYDWFGYGSKVIVTTKNEQLLDIHGFNKLRSVPELNYDEALELFSWHA 360
           IDTS QLQALAGGYDWFGYGSKVIVTT+NEQLLDIHGFNKLRSVPELNYDEALELFSWHA
Sbjct: 301 IDTSEQLQALAGGYDWFGYGSKVIVTTRNEQLLDIHGFNKLRSVPELNYDEALELFSWHA 360

Query: 361 FQSSRPPSEYLQLSIDAVNYCKNLPLALEVLGSFLYSTDQSKFKGILEEFAISNLDKDIQ 420
           FQ S PPSEYLQLS DAVNYCKNLPLALEVLGSFLYSTDQSKF+GILEEFAISNLDKDIQ
Sbjct: 361 FQCSHPPSEYLQLSKDAVNYCKNLPLALEVLGSFLYSTDQSKFRGILEEFAISNLDKDIQ 420

Query: 421 NLLQVSYDELEGDVQEMFLFISCFFVGEDKTMVETMLKSCGCLCWERGIKKLMNLSLFTI 480
           NLLQVSYDELEGDVQEMFLFISCFFVGEDKTMVETMLKSCGCLCWE+GIKKLMNLSL TI
Sbjct: 421 NLLQVSYDELEGDVQEMFLFISCFFVGEDKTMVETMLKSCGCLCWEKGIKKLMNLSLLTI 480

Query: 481 NKQNEVEMHDLIQQMGHTIARSKTSICPSEKKLLVGDEAMHVLDGIKEARAVKAIKLEFP 540
           NK N+VEMHDLIQQMGHTIARSKTSI  SEKKLLVGD+AMHV DGIKEARAVKAIKLEFP
Sbjct: 481 NKWNKVEMHDLIQQMGHTIARSKTSISASEKKLLVGDDAMHVFDGIKEARAVKAIKLEFP 540

Query: 541 KPTKLDIIDSTAFRKVKNLVVLKVKNVRSSKISTLDFLPNSLRWMSWSEFPFSSFPSSYS 600
           KPTKLDIIDS+AFRKVKNLVVLKVKNV SSKISTL+FLPNSLRWMSWSEFPFSSFPSSYS
Sbjct: 541 KPTKLDIIDSSAFRKVKNLVVLKVKNVISSKISTLEFLPNSLRWMSWSEFPFSSFPSSYS 600

Query: 601 MENLIQLKLPHSSIEHFGRAFMHCERLKQLDLSNSFFLEEIPDLSAAINLENLSLSGCIG 660
           MENLIQLKLPHSSIEHFGRAFM CE LKQLDLSNSFFLEEIPDLSAAINLENLSLSGCI 
Sbjct: 601 MENLIQLKLPHSSIEHFGRAFMRCESLKQLDLSNSFFLEEIPDLSAAINLENLSLSGCIS 660

Query: 661 LVKVHKSVGSLGKLVDLSLSSHVYGFKQFPSPLKLKSLKRFSTDHCTIVQGYPQFSEEMK 720
           LVKVHKSVGSLGKLVDLSLSSHVYGFKQFPSPLKLKSLKRFSTDHCTIVQGYPQFS+EMK
Sbjct: 661 LVKVHKSVGSLGKLVDLSLSSHVYGFKQFPSPLKLKSLKRFSTDHCTIVQGYPQFSQEMK 720

Query: 721 SSLEDLWFYSSSITELSSTIRYLTSLKDLTITDCKKLTTLPSTIYDLSKLTSIEVSQSDF 780
           SSLEDLWFYSSSITELSSTIRYLTSLKDLTITDCKKLTTLPSTIYDLSKLTSIEVSQSD 
Sbjct: 721 SSLEDLWFYSSSITELSSTIRYLTSLKDLTITDCKKLTTLPSTIYDLSKLTSIEVSQSDL 780

Query: 781 STFPSSYSCPSSLTHLTRLHLYENKITNLDFLETIAHVAPSLRELNL------------- 840
           STFPSSYSCPSSL HLTRLHLYENKITNLDFLETIAH APSLRELNL             
Sbjct: 781 STFPSSYSCPSSLPHLTRLHLYENKITNLDFLETIAHAAPSLRELNLSNNNFSILPSCIV 840

Query: 841 ------FLETFDCKLLEEIPKIPEGLISLGAEGCISLAKFPDNLADFISCDSEHVDGQFK 885
                 FLETFDCKLLEEIPKIPEGLISLGAEGCISLAKFPDNLADFIS DS H+     
Sbjct: 841 NFKSLRFLETFDCKLLEEIPKIPEGLISLGAEGCISLAKFPDNLADFISSDSVHISSPSL 899

BLAST of PI0000332 vs. NCBI nr
Match: XP_008455000.2 (PREDICTED: TMV resistance protein N-like isoform X2 [Cucumis melo])

HSP 1 Score: 1567.4 bits (4057), Expect = 0.0e+00
Identity = 818/1089 (75.11%), Postives = 884/1089 (81.18%), Query Frame = 0

Query: 1    MDRASGSFSSHFRCSFDVFLSFRGEDTRFNFTSHLDTALRQRGINVFI-DNKLTRGANFP 60
            MDRASGSFSSH R  FDVFLSFRGEDTRFNFTSHL TALRQRGINVFI D++LTRG NFP
Sbjct: 1    MDRASGSFSSH-RWRFDVFLSFRGEDTRFNFTSHLYTALRQRGINVFIDDSELTRGENFP 60

Query: 61   PSLLKAIEESKISVVIISENYASSSWCLNELVHIIMGNELRGQVVLPIFYKVNPSQVRTQ 120
             SLL+AIEESKISVVIISENYA+SSWCLNELV++IM  +LRGQVVLPIFYKVNPSQVRTQ
Sbjct: 61   SSLLRAIEESKISVVIISENYATSSWCLNELVYLIMCKKLRGQVVLPIFYKVNPSQVRTQ 120

Query: 121  NGAFGEAFAELEVRFFDKMQAWRDALTAISYMSGWVVLQKDDEANLIQKIVQQVWKKLTG 180
            NGAFGEAFA+LEVRFFDKMQAWR+ALT +S+MSGWV+LQ DDEA LIQ IV+ VWKKLT 
Sbjct: 121  NGAFGEAFAKLEVRFFDKMQAWREALTTVSFMSGWVLLQNDDEARLIQIIVRHVWKKLTC 180

Query: 181  STMQLPVTKYPVGIDRQVENLLSHVMIDGTRMVGLYGIGGMGKTTLAKALYNRIADDFEG 240
            ST+QL VTKYPVGIDRQV++LLSHV+ID TRMVGLYGIGGMGKTTLAKALYNR+AD FEG
Sbjct: 181  STVQLSVTKYPVGIDRQVKDLLSHVIIDETRMVGLYGIGGMGKTTLAKALYNRVADKFEG 240

Query: 241  CCFLANIREASKEHEGLVRLQEKLLYEILMDDFIRVGNLHRGINIIRNRLCSKKILLILD 300
            CCFLANIREASK+H+GLVRLQEKLLY+ILM DF+RVG++++GINIIRNRL S +ILLILD
Sbjct: 241  CCFLANIREASKQHDGLVRLQEKLLYDILMYDFVRVGDVYKGINIIRNRLYSIRILLILD 300

Query: 301  DIDTSVQLQALAGGYDWFGYGSKVIVTTKNEQLLDIHGFNKLRSVPELNYDEALELFSWH 360
            DIDTS QLQ LAGGYDWFG+GSKVIVTT+NEQLLDIHGF KL+ VP+L++ EALELFSWH
Sbjct: 301  DIDTSEQLQVLAGGYDWFGHGSKVIVTTRNEQLLDIHGFYKLKEVPQLHFGEALELFSWH 360

Query: 361  AFQSSRPPSEYLQLSIDAVNYCKNLPLALEVLGSFLYSTDQSKFKGILEEFAISNLDKDI 420
            AF +S PPSEY  L  DAVNYCKNLPLALEVLGSFLYSTDQSKFKGILEEFA SNL+KDI
Sbjct: 361  AFHNSCPPSEYSTLPEDAVNYCKNLPLALEVLGSFLYSTDQSKFKGILEEFANSNLNKDI 420

Query: 421  QNLLQVSYDELEGDVQEMFLFISCFFVGEDKTMVETMLKSCGCLCWERGIKKLMNLSLFT 480
            Q LLQ                                                       
Sbjct: 421  QKLLQ------------------------------------------------------- 480

Query: 481  INKQNEVEMHDLIQQMGHTIARSKTSICPSEKKLLVGDEAMHVLDGIKEARAVKAIKLEF 540
                                                            EARAVKAIK+EF
Sbjct: 481  ------------------------------------------------EARAVKAIKMEF 540

Query: 541  PKPTKLDIIDSTAFRKVKNLVVLKVKNVRSSKISTLDFLPNSLRWMSWSEFPFSSFPSSY 600
            P PT+LDIIDS AF KVKNL VLKVKNV  SKISTLD LPNSLRWMSWS FPFSSFPSSY
Sbjct: 541  PNPTELDIIDSNAFSKVKNLAVLKVKNVTFSKISTLDSLPNSLRWMSWSGFPFSSFPSSY 600

Query: 601  SMENLIQLKLPHSSIEHFGR-AFMHCERLKQLDLSNSFFLEEIPDLSAAINLENLSLSGC 660
            SMEN+IQLKLPHSSI+ F + AF HC+ LK+LDLSNS FLEEIPDLSAA NLE LSLSGC
Sbjct: 601  SMENIIQLKLPHSSIKRFEKEAFTHCKWLKELDLSNSIFLEEIPDLSAATNLEKLSLSGC 660

Query: 661  IGLVKVHKSVGSLGKLVDLSLSSHVYGFKQFPSPLKLKSLKRFSTDHCTIVQGYPQFSEE 720
              LVKVHKSVGSLGKLVDL +SSHVYGF+QFPSPLKLKSLKRFST HCTIV+GYPQFS+E
Sbjct: 661  ENLVKVHKSVGSLGKLVDLCISSHVYGFEQFPSPLKLKSLKRFSTYHCTIVRGYPQFSKE 720

Query: 721  MKSSLEDLWFYSSSITELSSTIRYLTSLKDLTITDCKKLTTLPSTIYDLSKLTSIEVSQS 780
            M+SSLE LWFY SSITELSSTIRYLTSLK L+ITDCK+LTTLPSTIYDLSKLTSIEVSQS
Sbjct: 721  MESSLEHLWFYRSSITELSSTIRYLTSLKILSITDCKELTTLPSTIYDLSKLTSIEVSQS 780

Query: 781  DFSTFPSSYSCPSSLTHLTRLHLYENKITNLDFLETIAHVAPSLRELNL----------- 840
            D STFP SYSCPSSL HLTRL LYENKITNLDFLETIAH APSLRELNL           
Sbjct: 781  DLSTFPFSYSCPSSLLHLTRLDLYENKITNLDFLETIAHAAPSLRELNLSNNNFSILPSC 840

Query: 841  --------FLETFDCKLLEEIPKIPEGLISLGAEGCISLAKFPDNLADFISCDSEHVDGQ 900
                    FLET DCKLL++IPKIPEGLI L A+GCISLAKFPDNLADFISCDSEHVDGQ
Sbjct: 841  IVNFKSLRFLETIDCKLLKKIPKIPEGLIYLDAQGCISLAKFPDNLADFISCDSEHVDGQ 900

Query: 901  FKQLILMNCDIPDWFSYKSRNNPMTIFMPSNDPRWEFKVFAPCVKFQVNHVDRNRYMDLE 960
            FKQLILMNCDIPDWFSYKSRNNP+T+ +PSNDP  E KVFA CVKFQVNHVD+++YMDLE
Sbjct: 901  FKQLILMNCDIPDWFSYKSRNNPITLLVPSNDPSSELKVFAACVKFQVNHVDQDQYMDLE 960

Query: 961  CKVLINDIQVWSSEEVPFLEESRRILIEASPYEYMWLLVLYPHIDFPLNWDDIIKRSQEF 1020
            CKV INDIQVWS EEVPF +ESR ILI+ASP+EYMWLLVLYPHI+F LN DDII RSQE 
Sbjct: 961  CKVFINDIQVWSYEEVPFHDESRSILIKASPHEYMWLLVLYPHINFRLNSDDIINRSQEI 985

Query: 1021 NLHQPSFGINSMGRDYNNCDVDDD-RRNHFGESIWRKFTVSFGVNSKFKDSEVSIKRCGV 1068
            NLHQPSFGINS+GRD NNC+VDDD RRNH GESIWRKFTVSFGV SKFKDSE+SIK CGV
Sbjct: 1021 NLHQPSFGINSIGRDNNNCNVDDDYRRNHIGESIWRKFTVSFGVTSKFKDSELSIKTCGV 985

BLAST of PI0000332 vs. NCBI nr
Match: XP_031737215.1 (TMV resistance protein N isoform X1 [Cucumis sativus])

HSP 1 Score: 1522.3 bits (3940), Expect = 0.0e+00
Identity = 778/872 (89.22%), Postives = 811/872 (93.00%), Query Frame = 0

Query: 1   MDRASGSFSSH-FRCSFDVFLSFRGEDTRFNFTSHLDTALRQRGINVFIDNKLTRGANFP 60
           MDRASGS SS  FR +FDVFLSFRGEDTR NFTSHL+ ALRQRGINVFIDN+++RG    
Sbjct: 1   MDRASGSSSSSCFRWTFDVFLSFRGEDTRSNFTSHLNMALRQRGINVFIDNRISRGQEIS 60

Query: 61  PSLLKAIEESKISVVIISENYASSSWCLNELVHIIMGNELRGQVVLPIFYKVNPSQVRTQ 120
            SL +AIEESKIS+VIIS+NYASSSWCLNELV IIM  ELRGQVVLPIFYKVNPSQVR Q
Sbjct: 61  ASLFEAIEESKISIVIISQNYASSSWCLNELVKIIMCKELRGQVVLPIFYKVNPSQVRKQ 120

Query: 121 NGAFGEAFAELEVRFFDKMQAWRDALTAISYMSGWVVLQKDDEANLIQKIVQQVWKKLTG 180
           NGAFGEAFAELEVRFFDKMQAW +ALTA+S+MSGWVVL+KDDEANLIQKIVQQVWKKLT 
Sbjct: 121 NGAFGEAFAELEVRFFDKMQAWGEALTAVSHMSGWVVLEKDDEANLIQKIVQQVWKKLTC 180

Query: 181 STMQLPVTKYPVGIDRQVENLLSHVMIDGTRMVGLYGIGGMGKTTLAKALYNRIADDFEG 240
           STMQLPVTKYPVGIDRQ ENLLSHVMIDGTRMVGL+GIGGMGKTTLAK LYNRIADDFEG
Sbjct: 181 STMQLPVTKYPVGIDRQFENLLSHVMIDGTRMVGLHGIGGMGKTTLAKTLYNRIADDFEG 240

Query: 241 CCFLANIREASKEHEGLVRLQEKLLYEILMDDFIRVGNLHRGINIIRNRLCSKKILLILD 300
           CCFLANIREASK+HEGLVRLQEKLLYEILMDDFIRV +L++GINIIRNRLCSKKILLILD
Sbjct: 241 CCFLANIREASKQHEGLVRLQEKLLYEILMDDFIRVSDLYKGINIIRNRLCSKKILLILD 300

Query: 301 DIDTSVQLQALAGGYDWFGYGSKVIVTTKNEQLLDIHGFNKLRSVPELNYDEALELFSWH 360
           DIDTS QLQ LAGGYDWFGYGSKVIVTT+NE LLDIHGFNKLRSVPELNY EALELFSWH
Sbjct: 301 DIDTSEQLQVLAGGYDWFGYGSKVIVTTRNEHLLDIHGFNKLRSVPELNYGEALELFSWH 360

Query: 361 AFQSSRPPSEYLQLSIDAVNYCKNLPLALEVLGSFLYSTDQSKFKGILEEFAISNLDKDI 420
           AFQ S PP+EYLQLS DAVNYCKNLPLALEVLGSFLYSTDQSKFKGILEEFAISNLDKDI
Sbjct: 361 AFQCSSPPTEYLQLSKDAVNYCKNLPLALEVLGSFLYSTDQSKFKGILEEFAISNLDKDI 420

Query: 421 QNLLQVSYDELEGDVQEMFLFISCFFVGEDKTMVETMLKSCGCLCWERGIKKLMNLSLFT 480
           QNLLQVSYDELEGDVQEMFLFISCFFVGEDKTMVETMLKSCGCLCWE+GI+KLMNLSL T
Sbjct: 421 QNLLQVSYDELEGDVQEMFLFISCFFVGEDKTMVETMLKSCGCLCWEKGIQKLMNLSLLT 480

Query: 481 INKQNEVEMHDLIQQMGHTIARSKTSICPSEKKLLVGDEAMHVLDGIKEARAVKAIKLEF 540
           IN+ N+VEMHDLIQQ+GHTIARSKTSI PSEKKLLVGD+AMHVLDGIK+ARAVKAIKLEF
Sbjct: 481 INQWNKVEMHDLIQQLGHTIARSKTSISPSEKKLLVGDDAMHVLDGIKDARAVKAIKLEF 540

Query: 541 PKPTKLDIIDSTAFRKVKNLVVLKVKNVRSSKISTLDFLPNSLRWMSWSEFPFSSFPSSY 600
           PKPTKLDIIDSTAFRKVKNLVVLKVKNV S KISTLDFLPNSLRWMSWSEFPFSSFPSSY
Sbjct: 541 PKPTKLDIIDSTAFRKVKNLVVLKVKNVISPKISTLDFLPNSLRWMSWSEFPFSSFPSSY 600

Query: 601 SMENLIQLKLPHSSIEHFGRAFMHCERLKQLDLSNSFFLEEIPDLSAAINLENLSLSGCI 660
           SMENLIQLKLPHS+I+HFGRAFMHCERLKQLDLSNSFFLEEIPDLSAAINLENLSLSGCI
Sbjct: 601 SMENLIQLKLPHSAIQHFGRAFMHCERLKQLDLSNSFFLEEIPDLSAAINLENLSLSGCI 660

Query: 661 GLVKVHKSVGSLGKLVDLSLSSHVYGFKQFPSPLKLKSLKRFSTDHCTIVQGYPQFSEEM 720
            LVKVHKSVGSL KL+DLSLSSHVYGFKQFPSPL+LKSLKRFSTDHCTI+QGYPQFS+EM
Sbjct: 661 SLVKVHKSVGSLPKLIDLSLSSHVYGFKQFPSPLRLKSLKRFSTDHCTILQGYPQFSQEM 720

Query: 721 KSSLEDLWFYSSSITELSSTIRYLTSLKDLTITDCKKLTTLPSTIYDLSKLTSIEVSQSD 780
           KSSLEDLWF SSSIT+LSSTIRYLTSLKDLTI DCKKLTTLPSTIYDLSKLTSIEVSQSD
Sbjct: 721 KSSLEDLWFQSSSITKLSSTIRYLTSLKDLTIVDCKKLTTLPSTIYDLSKLTSIEVSQSD 780

Query: 781 FSTFPSSYSCPSSLTHLTRLHLYENKITNLDFLETIAHVAPSLRELNL------------ 840
            STFPSSYSCPSSL  LTRLHLYENKITNLDFLETIAH APSLRELNL            
Sbjct: 781 LSTFPSSYSCPSSLPLLTRLHLYENKITNLDFLETIAHAAPSLRELNLSNNNFSILPSCI 840

Query: 841 -------FLETFDCKLLEEIPKIPEGLISLGA 853
                  FLETFDCK LEEIPKIPEGLISLGA
Sbjct: 841 VNFKSLRFLETFDCKFLEEIPKIPEGLISLGA 872

BLAST of PI0000332 vs. NCBI nr
Match: XP_016901542.1 (PREDICTED: TMV resistance protein N-like isoform X3 [Cucumis melo])

HSP 1 Score: 1506.5 bits (3899), Expect = 0.0e+00
Identity = 771/923 (83.53%), Postives = 830/923 (89.92%), Query Frame = 0

Query: 1   MDRASGSFSSHFRCSFDVFLSFRGEDTRFNFTSHLDTALRQRGINVFI-DNKLTRGANFP 60
           MDRASGSFSSH R  FDVFLSFRGEDTRFNFTSHL TALRQRGINVFI D++LTRG NFP
Sbjct: 1   MDRASGSFSSH-RWRFDVFLSFRGEDTRFNFTSHLYTALRQRGINVFIDDSELTRGENFP 60

Query: 61  PSLLKAIEESKISVVIISENYASSSWCLNELVHIIMGNELRGQVVLPIFYKVNPSQVRTQ 120
            SLL+AIEESKISVVIISENYA+SSWCLNELV++IM  +LRGQVVLPIFYKVNPSQVRTQ
Sbjct: 61  SSLLRAIEESKISVVIISENYATSSWCLNELVYLIMCKKLRGQVVLPIFYKVNPSQVRTQ 120

Query: 121 NGAFGEAFAELEVRFFDKMQAWRDALTAISYMSGWVVLQKDDEANLIQKIVQQVWKKLTG 180
           NGAFGEAFA+LEVRFFDKMQAWR+ALT +S+MSGWV+LQ DDEA LIQ IV+ VWKKLT 
Sbjct: 121 NGAFGEAFAKLEVRFFDKMQAWREALTTVSFMSGWVLLQNDDEARLIQIIVRHVWKKLTC 180

Query: 181 STMQLPVTKYPVGIDRQVENLLSHVMIDGTRMVGLYGIGGMGKTTLAKALYNRIADDFEG 240
           ST+QL VTKYPVGIDRQV++LLSHV+ID TRMVGLYGIGGMGKTTLAKALYNR+AD FEG
Sbjct: 181 STVQLSVTKYPVGIDRQVKDLLSHVIIDETRMVGLYGIGGMGKTTLAKALYNRVADKFEG 240

Query: 241 CCFLANIREASKEHEGLVRLQEKLLYEILMDDFIRVGNLHRGINIIRNRLCSKKILLILD 300
           CCFLANIREASK+H+GLVRLQEKLLY+ILM DF+RVG++++GINIIRNRL S +ILLILD
Sbjct: 241 CCFLANIREASKQHDGLVRLQEKLLYDILMYDFVRVGDVYKGINIIRNRLYSIRILLILD 300

Query: 301 DIDTSVQLQALAGGYDWFGYGSKVIVTTKNEQLLDIHGFNKLRSVPELNYDEALELFSWH 360
           DIDTS QLQ LAGGYDWFG+GSKVIVTT+NEQLLDIHGF KL+ VP+L++ EALELFSWH
Sbjct: 301 DIDTSEQLQVLAGGYDWFGHGSKVIVTTRNEQLLDIHGFYKLKEVPQLHFGEALELFSWH 360

Query: 361 AFQSSRPPSEYLQLSIDAVNYCKNLPLALEVLGSFLYSTDQSKFKGILEEFAISNLDKDI 420
           AF +S PPSEY  L  DAVNYCKNLPLALEVLGSFLYSTDQSKFKGILEEFA SNL+KDI
Sbjct: 361 AFHNSCPPSEYSTLPEDAVNYCKNLPLALEVLGSFLYSTDQSKFKGILEEFANSNLNKDI 420

Query: 421 QNLLQVSYDELEGDVQEMFLFISCFFVGEDKTMVETMLKSCGCLCWERGIKKLMNLSLFT 480
           Q LLQVSYDELEGDVQEMFLFISCFFVGEDKTMVE MLKSCGCLCWE GIKKLMNLSL T
Sbjct: 421 QKLLQVSYDELEGDVQEMFLFISCFFVGEDKTMVEMMLKSCGCLCWENGIKKLMNLSLLT 480

Query: 481 INKQNEVEMHDLIQQMGHTIARSKTSICPSEKKLLVGDEAMHVLDGIKEARAVKAIKLEF 540
           IN+ N+V+MHDLI+QMGHTIARS+T I  SEKK++V DEAMHVLDGI EARAVKAIK+EF
Sbjct: 481 INRINKVQMHDLIKQMGHTIARSRTFISHSEKKIMVEDEAMHVLDGINEARAVKAIKMEF 540

Query: 541 PKPTKLDIIDSTAFRKVKNLVVLKVKNVRSSKISTLDFLPNSLRWMSWSEFPFSSFPSSY 600
           P PT+LDIIDS AF KVKNL VLKVKNV  SKISTLD LPNSLRWMSWS FPFSSFPSSY
Sbjct: 541 PNPTELDIIDSNAFSKVKNLAVLKVKNVTFSKISTLDSLPNSLRWMSWSGFPFSSFPSSY 600

Query: 601 SMENLIQLKLPHSSIEHFGR-AFMHCERLKQLDLSNSFFLEEIPDLSAAINLENLSLSGC 660
           SMEN+IQLKLPHSSI+ F + AF HC+ LK+LDLSNS FLEEIPDLSAA NLE LSLSGC
Sbjct: 601 SMENIIQLKLPHSSIKRFEKEAFTHCKWLKELDLSNSIFLEEIPDLSAATNLEKLSLSGC 660

Query: 661 IGLVKVHKSVGSLGKLVDLSLSSHVYGFKQFPSPLKLKSLKRFSTDHCTIVQGYPQFSEE 720
             LVKVHKSVGSLGKLVDL +SSHVYGF+QFPSPLKLKSLKRFST HCTIV+GYPQFS+E
Sbjct: 661 ENLVKVHKSVGSLGKLVDLCISSHVYGFEQFPSPLKLKSLKRFSTYHCTIVRGYPQFSKE 720

Query: 721 MKSSLEDLWFYSSSITELSSTIRYLTSLKDLTITDCKKLTTLPSTIYDLSKLTSIEVSQS 780
           M+SSLE LWFY SSITELSSTIRYLTSLK L+ITDCK+LTTLPSTIYDLSKLTSIEVSQS
Sbjct: 721 MESSLEHLWFYRSSITELSSTIRYLTSLKILSITDCKELTTLPSTIYDLSKLTSIEVSQS 780

Query: 781 DFSTFPSSYSCPSSLTHLTRLHLYENKITNLDFLETIAHVAPSLRELNL----------- 840
           D STFP SYSCPSSL HLTRL LYENKITNLDFLETIAH APSLRELNL           
Sbjct: 781 DLSTFPFSYSCPSSLLHLTRLDLYENKITNLDFLETIAHAAPSLRELNLSNNNFSILPSC 840

Query: 841 --------FLETFDCKLLEEIPKIPEGLISLGAEGCISLAKFPDNLADFISCDSEHVDGQ 900
                   FLET DCKLL++IPKIPEGLI L A+GCISLAKFPDNLADFISCDSEHVDGQ
Sbjct: 841 IVNFKSLRFLETIDCKLLKKIPKIPEGLIYLDAQGCISLAKFPDNLADFISCDSEHVDGQ 900

Query: 901 FKQLILMNCDIPDWFSYKSRNNP 903
           FKQLILMNCDIPDWFSYKSRNNP
Sbjct: 901 FKQLILMNCDIPDWFSYKSRNNP 922

BLAST of PI0000332 vs. TAIR 10
Match: AT5G36930.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 453.8 bits (1166), Expect = 3.9e-127
Identity = 318/884 (35.97%), Postives = 486/884 (54.98%), Query Frame = 0

Query: 13  RCSFDVFLSFRGEDTRFNFTSHLDTALRQRGINVFIDN-KLTRGANFPPSLLKAIEESKI 72
           R ++DVF+SFRG D R NF SHL  +LR+ GI+ F+D+ +L RG    P LL AIE SKI
Sbjct: 11  RWTYDVFVSFRGADVRKNFLSHLYDSLRRCGISTFMDDVELQRGEYISPELLNAIETSKI 70

Query: 73  SVVIISENYASSSWCLNELVHIIMGNELR-GQVVLPIFYKVNPSQVRTQNGAFGEAFAEL 132
            +V+++++YASS+WCL+ELVHI+  ++     +V PIF  V+PS +R Q G++ ++F++ 
Sbjct: 71  LIVVLTKDYASSAWCLDELVHIMKSHKNNPSHMVFPIFLYVDPSDIRWQQGSYAKSFSKH 130

Query: 133 EVRF-FDKMQAWRDALTAISYMSGWVVLQKDDEANLIQKIVQQVWKKLTGSTMQLPVTKY 192
           +     +K++ WR+ALT ++ +SGW +  + +EA  I  I +++ K+L    + +P   Y
Sbjct: 131 KNSHPLNKLKDWREALTKVANISGWDIKNR-NEAECIADITREILKRLPCQYLHVP--SY 190

Query: 193 PVGIDRQVENLLSHVMI--DGTRMVGLYGIGGMGKTTLAKALYNRIADDFEGCCFLANIR 252
            VG+  +++++ S + I  DG R++ +YG+GG+GKTTLAK  +N  +  FEG  FL N R
Sbjct: 191 AVGLRSRLQHISSLLSIGSDGVRVIVIYGMGGIGKTTLAKVAFNEFSHLFEGSSFLENFR 250

Query: 253 EASKEHEGLVRLQEKLLYEILMDDFIRVGNLHRGINIIRNRLCSKKILLILDDIDTSVQL 312
           E SK+ EG   LQ +LL +IL  + I    L    + ++ R  SK++LL++DD+D   QL
Sbjct: 251 EYSKKPEGRTHLQHQLLSDILRRNDIEFKGLD---HAVKERFRSKRVLLVVDDVDDVHQL 310

Query: 313 QALAGGYDWFGYGSKVIVTTKNEQLLDIHGFNKLRSVPELNYDEALELFSWHAFQSSRPP 372
            + A   D FG+GS++I+TT+N  LL         S  EL+ DE+LELFSWHAF++S PP
Sbjct: 311 NSAAIDRDCFGHGSRIIITTRNMHLLKQLRAEGSYSPKELDGDESLELFSWHAFRTSEPP 370

Query: 373 SEYLQLSIDAVNYCKNLPLALEVLGSFLYSTDQSKFKGILEEFAISNLDKD-IQNLLQVS 432
            E+LQ S + V YC  LPLA+EVLG+FL      +++  L+   +  +  D IQ  LQ+S
Sbjct: 371 KEFLQHSEEVVTYCAGLPLAVEVLGAFLIERSIREWESTLK--LLKRIPNDNIQAKLQIS 430

Query: 433 YDELEGDVQEMFLFISCFFVGEDKTMVETMLKSCGCLCWERGIKKLMNLSLFTINKQNEV 492
           ++ L  + +++FL I+CFF+G D   V  +L  C  L  +  +  LM   L TI+  N +
Sbjct: 431 FNALTIEQKDVFLDIACFFIGVDSYYVACILDGCN-LYPDIVLSLLMERCLITIS-GNNI 490

Query: 493 EMHDLIQQMGHTIARS-KTSICPSEKKLLVGDEAMHVLDGIKEARAVKAIKLEFPKPTKL 552
            MHDL++ MG  I R      C    +L   ++ + VL       A++ + L      K 
Sbjct: 491 MMHDLLRDMGRQIVREISPKKCGERSRLWSHNDVVGVLKKKSGTNAIEGLSL------KA 550

Query: 553 DIID-----STAFRKVKNLVVLKVKNVRSSKISTLDFLPNSLRWMSWSEFPFSSFPSSYS 612
           D++D       AF K++ L +L+++ V  +   + +  P  LRW+ W  F    FP + S
Sbjct: 551 DVMDFQYFEVEAFAKMQELRLLELRYVDLN--GSYEHFPKDLRWLCWHGFSLECFPINLS 610

Query: 613 MENLIQLKLPHSSIEHFGRAF---MHCERLKQLDLSNSFFLEEIPDLSAAINLENLSLSG 672
           +E+L  L L +S+++ F +A         +K LDLS+S +L E PD S   N+E L L  
Sbjct: 611 LESLAALDLQYSNLKRFWKAQSPPQPANMVKYLDLSHSVYLRETPDFSYFPNVEKLILIN 670

Query: 673 CIGLVKVHKSVGSLG-KLVDLSLSSHVYGFKQFPSPL-KLKSLKRFSTDHCTIVQGYPQF 732
           C  LV VHKS+G L  KLV L+LSS +      P  + KLKSL+     +C+ ++     
Sbjct: 671 CKSLVLVHKSIGILDKKLVLLNLSSCI-ELDVLPEEIYKLKSLESLFLSNCSKLERLDDA 730

Query: 733 SEEMKSSLEDLWFYSSSITELSSTIRYLTSLKDLTITDCKKLTT------LPSTIYDLSK 792
             E++ SL  L    +++ E+ STI  L  LK L++  CK L +           + +S 
Sbjct: 731 LGELE-SLTTLLADFTALREIPSTINQLKKLKRLSLNGCKGLLSDDIDNLYSEKSHSVSL 790

Query: 793 LTSIEVSQSDFSTFPSSYSCP----------SSLTHLTRLHLYENKITNLDFLETIAHVA 852
           L  + +S   +    S   C            SL+ L  L L  N   N   L T     
Sbjct: 791 LRPVSLSGLTYMRILSLGYCNLSDELIPEDIGSLSFLRDLDLRGNSFCN---LPTDFATL 850

Query: 853 PSLRELNLFLETFDCKLLEEIPKIPEGLISLGAEGCISLAKFPD 864
           P+L EL L     DC  L+ I  +P  L+ L    CI L + PD
Sbjct: 851 PNLGELLL----SDCSKLQSILSLPRSLLFLDVGKCIMLKRTPD 867

BLAST of PI0000332 vs. TAIR 10
Match: AT5G36930.2 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 453.8 bits (1166), Expect = 3.9e-127
Identity = 318/884 (35.97%), Postives = 486/884 (54.98%), Query Frame = 0

Query: 13  RCSFDVFLSFRGEDTRFNFTSHLDTALRQRGINVFIDN-KLTRGANFPPSLLKAIEESKI 72
           R ++DVF+SFRG D R NF SHL  +LR+ GI+ F+D+ +L RG    P LL AIE SKI
Sbjct: 14  RWTYDVFVSFRGADVRKNFLSHLYDSLRRCGISTFMDDVELQRGEYISPELLNAIETSKI 73

Query: 73  SVVIISENYASSSWCLNELVHIIMGNELR-GQVVLPIFYKVNPSQVRTQNGAFGEAFAEL 132
            +V+++++YASS+WCL+ELVHI+  ++     +V PIF  V+PS +R Q G++ ++F++ 
Sbjct: 74  LIVVLTKDYASSAWCLDELVHIMKSHKNNPSHMVFPIFLYVDPSDIRWQQGSYAKSFSKH 133

Query: 133 EVRF-FDKMQAWRDALTAISYMSGWVVLQKDDEANLIQKIVQQVWKKLTGSTMQLPVTKY 192
           +     +K++ WR+ALT ++ +SGW +  + +EA  I  I +++ K+L    + +P   Y
Sbjct: 134 KNSHPLNKLKDWREALTKVANISGWDIKNR-NEAECIADITREILKRLPCQYLHVP--SY 193

Query: 193 PVGIDRQVENLLSHVMI--DGTRMVGLYGIGGMGKTTLAKALYNRIADDFEGCCFLANIR 252
            VG+  +++++ S + I  DG R++ +YG+GG+GKTTLAK  +N  +  FEG  FL N R
Sbjct: 194 AVGLRSRLQHISSLLSIGSDGVRVIVIYGMGGIGKTTLAKVAFNEFSHLFEGSSFLENFR 253

Query: 253 EASKEHEGLVRLQEKLLYEILMDDFIRVGNLHRGINIIRNRLCSKKILLILDDIDTSVQL 312
           E SK+ EG   LQ +LL +IL  + I    L    + ++ R  SK++LL++DD+D   QL
Sbjct: 254 EYSKKPEGRTHLQHQLLSDILRRNDIEFKGLD---HAVKERFRSKRVLLVVDDVDDVHQL 313

Query: 313 QALAGGYDWFGYGSKVIVTTKNEQLLDIHGFNKLRSVPELNYDEALELFSWHAFQSSRPP 372
            + A   D FG+GS++I+TT+N  LL         S  EL+ DE+LELFSWHAF++S PP
Sbjct: 314 NSAAIDRDCFGHGSRIIITTRNMHLLKQLRAEGSYSPKELDGDESLELFSWHAFRTSEPP 373

Query: 373 SEYLQLSIDAVNYCKNLPLALEVLGSFLYSTDQSKFKGILEEFAISNLDKD-IQNLLQVS 432
            E+LQ S + V YC  LPLA+EVLG+FL      +++  L+   +  +  D IQ  LQ+S
Sbjct: 374 KEFLQHSEEVVTYCAGLPLAVEVLGAFLIERSIREWESTLK--LLKRIPNDNIQAKLQIS 433

Query: 433 YDELEGDVQEMFLFISCFFVGEDKTMVETMLKSCGCLCWERGIKKLMNLSLFTINKQNEV 492
           ++ L  + +++FL I+CFF+G D   V  +L  C  L  +  +  LM   L TI+  N +
Sbjct: 434 FNALTIEQKDVFLDIACFFIGVDSYYVACILDGCN-LYPDIVLSLLMERCLITIS-GNNI 493

Query: 493 EMHDLIQQMGHTIARS-KTSICPSEKKLLVGDEAMHVLDGIKEARAVKAIKLEFPKPTKL 552
            MHDL++ MG  I R      C    +L   ++ + VL       A++ + L      K 
Sbjct: 494 MMHDLLRDMGRQIVREISPKKCGERSRLWSHNDVVGVLKKKSGTNAIEGLSL------KA 553

Query: 553 DIID-----STAFRKVKNLVVLKVKNVRSSKISTLDFLPNSLRWMSWSEFPFSSFPSSYS 612
           D++D       AF K++ L +L+++ V  +   + +  P  LRW+ W  F    FP + S
Sbjct: 554 DVMDFQYFEVEAFAKMQELRLLELRYVDLN--GSYEHFPKDLRWLCWHGFSLECFPINLS 613

Query: 613 MENLIQLKLPHSSIEHFGRAF---MHCERLKQLDLSNSFFLEEIPDLSAAINLENLSLSG 672
           +E+L  L L +S+++ F +A         +K LDLS+S +L E PD S   N+E L L  
Sbjct: 614 LESLAALDLQYSNLKRFWKAQSPPQPANMVKYLDLSHSVYLRETPDFSYFPNVEKLILIN 673

Query: 673 CIGLVKVHKSVGSLG-KLVDLSLSSHVYGFKQFPSPL-KLKSLKRFSTDHCTIVQGYPQF 732
           C  LV VHKS+G L  KLV L+LSS +      P  + KLKSL+     +C+ ++     
Sbjct: 674 CKSLVLVHKSIGILDKKLVLLNLSSCI-ELDVLPEEIYKLKSLESLFLSNCSKLERLDDA 733

Query: 733 SEEMKSSLEDLWFYSSSITELSSTIRYLTSLKDLTITDCKKLTT------LPSTIYDLSK 792
             E++ SL  L    +++ E+ STI  L  LK L++  CK L +           + +S 
Sbjct: 734 LGELE-SLTTLLADFTALREIPSTINQLKKLKRLSLNGCKGLLSDDIDNLYSEKSHSVSL 793

Query: 793 LTSIEVSQSDFSTFPSSYSCP----------SSLTHLTRLHLYENKITNLDFLETIAHVA 852
           L  + +S   +    S   C            SL+ L  L L  N   N   L T     
Sbjct: 794 LRPVSLSGLTYMRILSLGYCNLSDELIPEDIGSLSFLRDLDLRGNSFCN---LPTDFATL 853

Query: 853 PSLRELNLFLETFDCKLLEEIPKIPEGLISLGAEGCISLAKFPD 864
           P+L EL L     DC  L+ I  +P  L+ L    CI L + PD
Sbjct: 854 PNLGELLL----SDCSKLQSILSLPRSLLFLDVGKCIMLKRTPD 870

BLAST of PI0000332 vs. TAIR 10
Match: AT2G16870.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 417.5 bits (1072), Expect = 3.1e-116
Identity = 311/956 (32.53%), Postives = 491/956 (51.36%), Query Frame = 0

Query: 7   SFSSHFRCSFDVFLSFRGEDTRFNFTSHLDTALRQRGINVFIDNKLTRGANFPPSLLKAI 66
           S SS    ++ VF SF G D R +F SH        GI +F D K+ R     PSL + I
Sbjct: 3   SSSSSRTWNYRVFASFHGPDVRKSFLSHFRKQFICNGITMFDDQKIVRSQTIAPSLTQGI 62

Query: 67  EESKISVVIISENYASSSWCLNELVHIIMGNELRGQVVLPIFYKVNPSQVRTQNGAFGEA 126
            ESKIS+VI+S+NYASS+WCLNEL+ I+   E  GQ+V+ +FY V+PS VR Q G FG  
Sbjct: 63  RESKISIVILSKNYASSTWCLNELLEILKCREDIGQIVMTVFYGVDPSDVRKQTGEFGTV 122

Query: 127 FAELEVRFFDK-MQAWRDALTAISYMSGWVVLQKDDEANLIQKIVQQVWKKLTGSTMQLP 186
           F +   R  +K  + W  AL  +  ++G   L  D+EA +I+KI + V +KL       P
Sbjct: 123 FNKTCARRTEKERRNWSQALNVVGNIAGEHFLNWDNEAEMIEKIARDVSEKLN----MTP 182

Query: 187 VTKYP--VGIDRQVENL--LSHVMIDGTRMVGLYGIGGMGKTTLAKALYNRIA-DDFEGC 246
            + +   VGI+  ++ +  L     DG ++VG++G  G+GKTT+A+AL++ +    F+  
Sbjct: 183 SSDFDGMVGIEAHLKEMEVLLDFDYDGVKIVGIFGPAGIGKTTIARALHSLLLFKKFQLT 242

Query: 247 CFLANIREA----SKEHEGLVRLQEKLLYEILMDDFIRVGNLHRGINIIRNRLCSKKILL 306
           CF+ N+R +      E+   +RLQE LL +IL  D +R+ +L      ++ RLC  K+L+
Sbjct: 243 CFVDNLRGSYPIGIDEYGLKLRLQEHLLSKILNQDGMRISHL----GAVKERLCDMKVLI 302

Query: 307 ILDDIDTSVQLQALAGGYDWFGYGSKVIVTTKNEQLLDIHGFNKLRSVPELNYDEALELF 366
           ILDD++   QL+ALA    WFG GS+VIVTT+N+++L  HG + +  V   + ++A+E+ 
Sbjct: 303 ILDDVNDVKQLEALANDTTWFGPGSRVIVTTENKEILQRHGIDNMYHVGFPSDEKAMEIL 362

Query: 367 SWHAFQSSRPPSEYLQLSIDAVNYCKNLPLALEVLGSFLYSTDQSKFKGILEEFAISNLD 426
             +AF+ S P   +  L+      C NLPL L V+GS L    + ++K ++     + +D
Sbjct: 363 CGYAFKQSSPRPGFNYLAQKVTWLCGNLPLGLRVVGSSLRGKKEDEWKSVIRRLD-TIID 422

Query: 427 KDIQNLLQVSYDELEGDVQEMFLFISCFFVGEDKTMVETMLKSCGCLCWERGIKKLMNLS 486
           +DI+++L+V Y+ L  + Q +FL I+ FF  +D  +V+ ML     L    G+K L+N S
Sbjct: 423 RDIEDVLRVGYESLHENEQSLFLHIAVFFNCKDVDLVKAMLADDN-LDIAHGLKILVNKS 482

Query: 487 LFTINKQNEVEMHDLIQQMGHTIARSKTSICPSEKKLLV-GDEAMHVLDGIKEARAVKAI 546
           L  I+   E+ MH L+QQ+G      +    P ++ +L    E  +VL+  K    V  I
Sbjct: 483 LIYISTTGEIRMHKLLQQVGRQAINRQE---PWKRLILTNAQEICYVLENDKGTGVVSGI 542

Query: 547 KLEFPKPTKLDIIDSTAFRKVKNLVVLKVKNVRSSKISTLDF-----LPNSLRWMSWSEF 606
             +    +++ I+ + A R++ NL  L V   R    + +        P  LR + W  +
Sbjct: 543 SFDTSGISEV-ILSNRALRRMSNLRFLSVYKTRHDGNNIMHIPEDMKFPPRLRLLHWEAY 602

Query: 607 PFSSFPSSYSMENLIQLKLPHSSIEHFGRAFMHCERLKQLDLSNSFFLEEIPDLSAAINL 666
           P  S P  + +ENL++L +  S +E           LK++DLS S  L+E+PDLS A NL
Sbjct: 603 PSKSLPLGFCLENLVELNMKDSQLEKLWEGTQLLTNLKKMDLSRSVHLKELPDLSNATNL 662

Query: 667 ENLSLSGCIGLVKVHKSVGSLGKLVDLSLSSHVYGFKQFPSPLKLKSLKRFSTDHCTIVQ 726
           E L L  C  LV++ KS+G+L KL +L +++ +   +  P+ + L SL+  +   C+ ++
Sbjct: 663 ERLELCDCRALVELPKSIGNLHKLENLVMANCI-SLEVIPTHINLASLEHITMTGCSRLK 722

Query: 727 GYPQFSEEMKSSLEDLWFYSSSITELSSTIRYLTSLKDLTITDCKKLTTLPSTIYDLSKL 786
            +P FS    +++E L    +S+ E+ ++IR+ +SL D  I   K    L S  Y   K+
Sbjct: 723 TFPDFS----TNIERLLLIGTSVEEVPASIRHWSSLSDFCI---KNNEDLKSLTYFPEKV 782

Query: 787 TSIEVSQSDFSTFPSSYSCPSSLTHLTRLHLYE-NKITNLDFLETIAHVAPSLRELNLFL 846
             +++S +D    P    C      L  L +    K+T+L          P L      L
Sbjct: 783 ELLDLSYTDIEKIP---DCIKGFHGLKSLDVAGCRKLTSL----------PELPMSLGLL 842

Query: 847 ETFDCKLLEEIP-KIPEGLISLGAEGCISLAKFPDNLADFISCDSEHVDGQFKQLILMNC 906
              DC+ LE I   +      L    C  L +    L     C ++ +DG      L   
Sbjct: 843 VALDCESLEIITYPLNTPSARLNFTNCFKLGEESRRLI-IQRCATQFLDG---YACLPGR 902

Query: 907 DIPDWFSYK-SRNNPMTIFMPSNDPRWEFKVFAPCVKFQVNHVDRN-RYMDLECKV 943
            +PD F+ + S NN + I + S      FK F  CV    N    +  + D+ C V
Sbjct: 903 VMPDEFNQRTSGNNSLNIRLSSA----SFK-FKACVVISPNQQQHSWEHTDIRCIV 914

BLAST of PI0000332 vs. TAIR 10
Match: AT5G17680.1 (disease resistance protein (TIR-NBS-LRR class), putative )

HSP 1 Score: 412.9 bits (1060), Expect = 7.7e-115
Identity = 303/900 (33.67%), Postives = 467/900 (51.89%), Query Frame = 0

Query: 4   ASGSFSSHFRCSFDVFLSFRGEDTRFNFTSHLDTALRQRGINVFIDN-KLTRGANFPPSL 63
           +S S SS      DVF+SFRGED R  F SHL     + GI  F D+  L RG +  P L
Sbjct: 6   SSSSSSSSTVWKTDVFVSFRGEDVRKTFVSHLFCEFDRMGIKAFRDDLDLQRGKSISPEL 65

Query: 64  LKAIEESKISVVIISENYASSSWCLNELVHIIMGNELRGQVVLPIFYKVNPSQVRTQNGA 123
           + AI+ S+ ++V++S NYA+SSWCL+EL+ I+   E     ++PIFY+V+PS VR Q G+
Sbjct: 66  IDAIKGSRFAIVVVSRNYAASSWCLDELLKIM---ECNKDTIVPIFYEVDPSDVRRQRGS 125

Query: 124 FGEAFAELEVRFFDKMQAWRDALTAISYMSGWVVLQKDDEANLIQKIVQQVWKKLTGSTM 183
           FGE       +  +K+  W++AL  ++ +SG      DD + LI+KIV+ +  KL  ++ 
Sbjct: 126 FGEDVESHSDK--EKVGKWKEALKKLAAISGEDSRNWDD-SKLIKKIVKDISDKLVSTSW 185

Query: 184 QLPVTKYPVGIDRQVENLLSHVMI--DGTRMVGLYGIGGMGKTTLAKALYNRIADDFEGC 243
               +K  +G+   ++ L S + I     RM+G++G+GG+GKTT+AK LYN+++  F+  
Sbjct: 186 D--DSKGLIGMSSHMDFLQSMISIVDKDVRMLGIWGMGGVGKTTIAKYLYNQLSGQFQVH 245

Query: 244 CFLANIREASKEHEGLVRLQEKLLYEILMDDFIRVGNLHRGINIIRNRLCSKKILLILDD 303
           CF+ N++E    + G+ RLQ + L  +  +      +     NII+ R   K + ++LDD
Sbjct: 246 CFMENVKEVCNRY-GVRRLQVEFLCRMFQERDKEAWSSVSCCNIIKERFRHKMVFIVLDD 305

Query: 304 IDTSVQLQALAGGYDWFGYGSKVIVTTKNEQLLDIHGFNKLRSVPELNYDEALELFSWHA 363
           +D S QL  L     WFG GS++IVTT++  LL  HG N +  V  L   EAL+LF  +A
Sbjct: 306 VDRSEQLNELVKETGWFGPGSRIIVTTRDRHLLLSHGINLVYKVKCLPKKEALQLFCNYA 365

Query: 364 FQSS-RPPSEYLQLSIDAVNYCKNLPLALEVLGSFLYSTDQSKFKGILEEFAISNLDKDI 423
           F+     P  + +LS+ AVNY   LPLAL VLGSFLY   Q +++  L     +    DI
Sbjct: 366 FREEIILPHGFEELSVQAVNYASGLPLALRVLGSFLYRRSQIEWESTLARLK-TYPHSDI 425

Query: 424 QNLLQVSYDELEGDVQEMFLFISCFFVGEDKTMVETMLKSCGCLCWERGIKKLMNLSLFT 483
             +L+VSYD L+   + +FL+ISCF+  +    V  +L  CG    E GI  L   SL  
Sbjct: 426 MEVLRVSYDGLDEQEKAIFLYISCFYNMKQVDYVRKLLDLCGYAA-EIGITILTEKSLI- 485

Query: 484 INKQNEVEMHDLIQQMGHTIARSKTSICPSEKKLLVGDEAM-HVLDGIKEARAVKAIKLE 543
           +     V++HDL++QMG  + R +    P+++ LL   E + H+L      + V+ I L 
Sbjct: 486 VESNGCVKIHDLLEQMGRELVRQQAVNNPAQRLLLWDPEDICHLLSENSGTQLVEGISLN 545

Query: 544 FPKPTKLDIIDSTAFRKVKNLVVLKVKNV------RSSKISTLDFLPNSLRWMSWSEFPF 603
             + +++   D  AF  + NL +L   ++      R    + L +LP  LR++ W  +P 
Sbjct: 546 LSEISEVFASD-RAFEGLSNLKLLNFYDLSFDGETRVHLPNGLSYLPRKLRYLRWDGYPL 605

Query: 604 SSFPSSYSMENLIQLKLPHSSIEHFGRAFMHCERLKQLDLSNSFFLEEIPDLSAAINLEN 663
            + PS +  E L++L + +S++E           LK++DLS   +L E+PDLS A NLE 
Sbjct: 606 KTMPSRFFPEFLVELCMSNSNLEKLWDGIQPLRNLKKMDLSRCKYLVEVPDLSKATNLEE 665

Query: 664 LSLSGCIGLVKVHKSVGSLGKLVDLSLSSHVYGFKQFPSPLKLKSLKRFSTDHCTIVQGY 723
           L+LS C  LV+V  S+ +L  L    L++ +   K  P  + LKSL+      C+ ++ +
Sbjct: 666 LNLSYCQSLVEVTPSIKNLKGLSCFYLTNCIQ-LKDIPIGIILKSLETVGMSGCSSLKHF 725

Query: 724 PQFSEEMKSSLEDLWFYSSSITELSSTIRYLTSLKDLTITDCKKLTTLPSTIYDLSKLTS 783
           P+ S   +     L+  S+ I EL S+I  L+ L  L ++DC++L TLPS +  L  L S
Sbjct: 726 PEISWNTRR----LYLSSTKIEELPSSISRLSCLVKLDMSDCQRLRTLPSYLGHLVSLKS 785

Query: 784 IEVSQSDFSTFPSSYSCPSSLTHLTRLHLYE--------------NKITNLDFLETIAHV 843
           + +            + P +L +LT L   E                I  L   ET    
Sbjct: 786 LNLDGC-----RRLENLPDTLQNLTSLETLEVSGCLNVNEFPRVSTSIEVLRISETSIEE 845

Query: 844 APSLRELNLF-LETFDCKLLEEIPKIP------EGLISLGAEGCISLAKFPDNLADFISC 872
            P+ R  NL  L + D    + +  +P        L  L   GC  L  FP  +   +SC
Sbjct: 846 IPA-RICNLSQLRSLDISENKRLASLPVSISELRSLEKLKLSGCSVLESFPLEICQTMSC 881

BLAST of PI0000332 vs. TAIR 10
Match: AT5G18360.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 407.5 bits (1046), Expect = 3.2e-113
Identity = 279/780 (35.77%), Postives = 434/780 (55.64%), Query Frame = 0

Query: 18  VFLSFRGEDTRFNFTSHLDTALRQRGINVFIDNKLTRGANFPPSLLKAIEESKISVVIIS 77
           VF SF G+D R  F SHL    R++GI  FIDN + R       L++AI ES+I+VV++S
Sbjct: 18  VFPSFSGKDVRRTFLSHLLKEFRRKGIRTFIDNDIKRSQMISSELVRAIRESRIAVVVLS 77

Query: 78  ENYASSSWCLNELVHIIMGNELRGQVVLPIFYKVNPSQVRTQNGAFGEAFAELEVRFFDK 137
             YASSSWCLNELV I    +   Q+++P+FY+V+PS VR + G FG+AF E   R  D+
Sbjct: 78  RTYASSSWCLNELVEI----KKVSQMIMPVFYEVDPSDVRKRTGEFGKAFEEACERQPDE 137

Query: 138 --MQAWRDALTAISYMSGWVVLQKDDEANLIQKIVQQVWKKLTGSTMQLPVTKYPVGIDR 197
              Q WR+AL  I+ ++G      D+EA+LI KI   +  +L  ST+        VGID 
Sbjct: 138 EVKQKWREALVYIANIAGESSQNWDNEADLIDKIAMSISYEL-NSTLSRDSYNL-VGIDN 197

Query: 198 QVENLLSHVMIDGT--RMVGLYGIGGMGKTTLAKALYNRIADDFEGCCFLANIREASKEH 257
            +  L S + ++ T  +MVG++G  G+GKTT+A+AL+NR++++F+   F+ N++ +S+  
Sbjct: 198 HMRELDSLLCLESTEVKMVGIWGPAGIGKTTIARALFNRLSENFQHTIFMENVKGSSRTS 257

Query: 258 E-----GLVRLQEKLLYEILMDDFIRVGNLHRGINIIRNRLCSKKILLILDDIDTSVQLQ 317
           E       +RLQE+ L E++    +++ +L     +++ RL   K+L++LDD+D   QL 
Sbjct: 258 ELDAYGFQLRLQEQFLSEVIDHKHMKIHDL----GLVKERLQDLKVLVVLDDVDKLEQLD 317

Query: 318 ALAGGYDWFGYGSKVIVTTKNEQLLDIHGFNKLRSVPELNYDEALELFSWHAFQSSRPPS 377
           AL     WFG GS++IVTT+N+QLL  HG   +  +   +  ++L++F  +AF  S  P 
Sbjct: 318 ALVKQSQWFGSGSRIIVTTENKQLLRAHGITCIYELGFPSRSDSLQIFCQYAFGESSAPD 377

Query: 378 EYLQLSIDAVNYCKNLPLALEVLGSFLYSTDQSKFKGILEEFAISNLDKDIQNLLQVSYD 437
             ++L+ +       LPLAL+VLGS L    + + K  L     S L++DI+N+L+V YD
Sbjct: 378 GCIELATEITKLAGYLPLALKVLGSSLRGMSKDEQKSALPRLRTS-LNEDIRNVLRVGYD 437

Query: 438 ELEGDVQEMFLFISCFFVGEDKTMVETMLKSCGCLCWERGIKKLMNLSLFTINKQNE-VE 497
            +    + +FL I+C F GE+   V+ +L S G L    G++ L + SL  I++ N  + 
Sbjct: 438 GIHDKDKVIFLHIACLFNGENVDYVKQILASSG-LDVTFGLQVLTSRSLIHISRCNRTIT 497

Query: 498 MHDLIQQMGHTIARSKTSICPSEKKLLV-GDEAMHVLDGIKEARAVKAIKLEFPKPTKLD 557
           MH+L++Q+G  I   ++   P +++ L+   E   VL       AV  I L+  K  +L 
Sbjct: 498 MHNLLEQLGREIVCEQSIAEPGKRQFLMDASEIYDVLADNTGTGAVLGISLDISKINEL- 557

Query: 558 IIDSTAFRKVKNLVVLKVKNVRSSKIST-------LDFLPNSLRWMSWSEFPFSSFPSSY 617
            ++  AF  + NL+ L+     SSK          LD+LP  LR + W  FP +S P S+
Sbjct: 558 FLNERAFGGMHNLLFLRFYKSSSSKDQPELHLPRGLDYLPRKLRLLHWDAFPMTSMPLSF 617

Query: 618 SMENLIQLKLPHSSIEHFGRAFMHCERLKQLDLSNSFFLEEIPDLSAAINLENLSLSGCI 677
             + L+ + +  S +E           LKQ+DLS S  L+EIPDLS A+N+E L LS C 
Sbjct: 618 CPQFLVVINIRESQLEKLWEGTQPLRSLKQMDLSKSENLKEIPDLSKAVNIEELCLSYCG 677

Query: 678 GLVKVHKSVGSLGKLVDLSLSSHVYGFKQFPSPLKLKSLKRFSTDHCTIVQGYPQFSEEM 737
            LV +  S+ +L KLV L +  +    +  P  + L+SL   + D C+ ++ +P    E+
Sbjct: 678 SLVMLPSSIKNLNKLVVLDM-KYCSKLEIIPCNMDLESLSILNLDGCSRLESFP----EI 737

Query: 738 KSSLEDLWFYSSSITELSSTIRYLTSLKDLTITDCKKLTT---LPSTI--YDLSKLTSIE 775
            S +  L    ++I E+ +T+     L  L ++ CK L T   LP TI   DLS+ T IE
Sbjct: 738 SSKIGFLSLSETAIEEIPTTVASWPCLAALDMSGCKNLKTFPCLPKTIEWLDLSR-TEIE 778