PI0000230 (gene) Melon (PI 482460) v1

Overview


Name	PI0000230
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	Malic enzyme
Location	chr06: 2244192 .. 2257383 (-)
RNA-Seq Expression	PI0000230
Synteny	PI0000230

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTACACCTGTCCCCTCCATCTCCTTGGTTCTCAGTCTCGCCCAATCTCACTCCGAGAAGCTGCTCTGCTCTGAATCTCTCCAGTTTTCGTGGAGAAGCCATTGAAATCAGCTAAATGACGAAGTGGAATTCACTCTCTACTTCGCTTATGAAGCGTCTCAAGCTTCACTACCAGATGACCAACAGTATTCCAGCCTTAGCTCAGGCCAGATCTTTCACCACAAGCGAAGGACATCGTCCCACTATTGTTCATAAACGTAGCCTTGATATTCTTCACGATCCGTGGTTTAACAAAGTAAGCCTTTCGATTCTCGTTCGATTTCGCTATCATTTCTTAGTTGATTTGATTCTTTGTGAGATGCGGTACTTGACATCGTTTTTTAACCGGAGTTATTTGTTATTTATTTATTTTTTAATTGGAATCTGTGCGTGCTTCAGTGATTTGCGAAGATGATAATCTGTATTTGGAATTAAGATTAGAGGGATTGATCAATTATCTGTTGTAGTTAAGTAGCGAAAACACTTGTTCTTAGAAATTGTCTGACAAAATAATGAAGTACTACTCAAATTCGTCGATAAATCTCCAAGACTCTAACTCACTATATGATTATTGACAACGGTTCTGATTCTTGAGCATCCGAATGTTATGGTTGGATTCTGGATCGAGTTGGGTTAATTTTGTGAAGAATTAATTATACAGAGTTCAGCTTCCTTTTATTAGTTGTACTAACTACTGAGTACTGGGTTTGCACGACAAAGTTTGGGTATCCATGATCATTAGATCAGTGTTCACTGTTTATAAAATTCTAGCCAATTATATGGCGTGTGAAAATATGAATTGGGTATTAAGTTCTGCCCAATTATTGGCTGAAGTTAAAGAATACTGTATGGTCGGAGCGGATTCTGTTTATTTAATTTTAAATTATCGGTTAATGCATTTTTTTTTTGTTTGGAATGATACGATTATTGAGCTTTCATGGTGAAGAAAAGAGAGAGATGTCACAAGTGAGGGTCTACATACGTTATTTCTAATGTTAAACTGTGTACTTGGCATTTGAAAATAATATCATAAAAAACTATCCCTCTGTTTTTTCCCAATAATAACTTTATCATACGGAAAGAATCCTATTCATTATTTTGTAGGTATTGATTTGATAGAGTTTTTGTTCACTTTCATGGATATGAAATTGGAAAAAAATAGTGTTGTTCCCAAATACTAGATCAATTCTTGTCAAATTCAACTTATATTACTATCATAAAAAACTATCCCTCTTTCTTCCCCCAACAACTTTCTCATATGGAAAGAATGAAATCGTCATTTTGTACGTATTGATATGACGGAATTTTTGTTCACTTTCATGGGTATGAAATGAAAAAAAGTGCTATCCCCAAATTCTAGATCAATTCTTGTCAACTATTACTGTTTAATGACTTCTTCATTTCATTGAAAGAAGGGAAAGGATTTACTTTGAAGAATGCCTTTTCCTTTTCAAATGGTTCTCGTATCATATATACTTCCAACCCAAAGAAGTACAAAACTATTATAGGATTTGGAAAATTGAATGTAATGTGATTTGGAGCTAGTTTGATCTGTATTTCGATTCTTATTGCTTTGACATAAGTGCCATTTATGGACCCATTCTTCTTTATCATCATAGTAGGGAACTGCCTTTACAATGACAGAACGTGACAGGCTTGATCTTCGAGGACTTCTTCCACCAAATGTAATGTCTTCGGAGCAGCAAATTGAACGATTTAGTAAGTTCTTAAGATATTTATATTTTTTAATTTTTTAATTTTCCTTGGAGGCATTGAGAAAGACCATTTATTTTATATGTGTTATTGCTACCCTTAAAATTTTCTTCTTCTTATCATTTTTATTTTGGGGTATTAGAAACAACTTTGTGTGCATCAGTGCCTGTTAATTGATATGTGGTTTCAACATCGTTTACATGCATGCCTTAAGAGAGTGTACGAGCATGGTAACAATGAAAAAAGTGCTCTTCCTCCAGCGTTGTCTACTTTAATTTTAGTTAATCTTATGCTTTTACCTGTTAAATACTTCATAAATTTGAAATGCTTGTTAGCAAAATTTCTCTTTGTCATCGAACAAGATTATCCTTGTCATCGCTGGTGCACATTCAATATAGCAGCTCTGAATTCCTTCCATTTAAATTGGAGCAACATTCCTTCAGTTGTTTACTATAGATCTCAAGTTTATAGGTTGTATCTCAATTTACATTTCATTAAATTTCTTGCTGTTTGAATTCTCGATTCATCTTTATTCAATGCTTTTTTCTGGTGTAAAAATATTCAACCTATCAATACTTTCAGCTTGAATTTCCTTGTATCAAATTGGAAGTCCATTTTGCAACACCTTTACCGGCCCCTTTTGTAACACTTTGCACATTTCATTCATCAATGAAATTGTTTCTTTAATAAAAAAAATTCTTAAATTTATCTTCTAGATAATATGCAGCTTCAACTTTCCATCTTATTTTATTTTGATCTTGTATTTGTACTTGTAAGATCAACACCGGTTTTCTTGTTGTTCTTGTCGCGGTTTCTTCTTTTAAAATTTAAAAAACAAATTCTTCGTAGCTTTGACCTGCATGTCATAACTCTAGATAATTTACAGTGTTTGACCTGAAGAGGCTTGAAGTGCAAGCAAGAGATGGGCCTTCAGATCCAAACGCTTTGGCCAAATGGCGAATTCTGAACAGATTACACGACAGAAATGAAACAATGTACTATAAAGTGAGTTAAGGTTGCTATCTATCAGCGACGACAATTTTATTGACTTGTTCTCAATGAATTCATGATCTAAACTAGCTAGTTTGTGCTTTACAGAATGTGACCTCTAAAGGGGAAGGGTATTTCTTCTAATTGAATAAATACATTTTCATTAGGTTAAATTGTTTTCTAATGTTTGTTTAGTAAGAGATCTCCTCTAAGGTGTTTTTTTTCTTTAAGGAAGAATTAAATATGCCACAAAATTTTAAAATATTGTTAGACATTGTCTATCATATGTGTTCAATGATGTCTCCCATTCTTCTACTATTGAGTCGTTGACTAGGTTCACAATTTAGTAGAAATGCTTCAATTTTATAGTTGGTCGTCCTATCAGGTTAAATCACCAATCAACCAAAAGTTTGAGTTGATGGGATACACTAAATTTTGTTACATCTAACACTTCAAGGCATCACATTCAAGTTATTCTAAAAAACATGAAAAATAACAAATTTCACACGGTGTATTTTCTCAGCATGCGTATTTTCTTATACTGTAATAGCACTGTCGTATTTTTTAATTAATGCAGTTACTTGTCTTATGAATTATTTGCCACATCACAAGCTATCTAATTAAATGTGACTTTGATAGGTTTTGATTGCTCACATTGAGGAGTATGCGCCCATAGTATACACGCCCACTGTAGGTCTCGTTTGCCAAAACTATAGTGGCTTGTTTAGAAGGCCAAGGGGAATGTATTTCAGTGCAGAAGATCGTGGAGAAATGATGTCTATGGTTTATAATTGGCCTGCTGATCAGGTTTTCTTCAACTAACTTCAATGCTCATACCAGACAATTCATTGAACCAATTCAAATTGTTCGGATGCCAAATACTTTAGACTTTTTGGAATTGGAACTCTGTATATAAAGTAAATTGAAAATCTAAAAACTTTTTTCAGGTTGATATGATTGTTGTCACCGATGGAAGTAGAATATTAGGACTTGGAGATCTTGGAGTTCATGGGATTGGGATTGCCATAGGAAAGTTGGATTTATATGTTGCTGCTGCCGGGATAAATCCTCAGAGGGTAGGTTCTTGTCTTGCTTCTAGACATCTTATGTGATTAGTTCCCTGGCATGATTAGCTAGTAGCTGGTTATGTTTTGGAGTTTATTGGTTCAGAAACTTTTATTCACGTTCAAAACATTATTTAGACAAGGCCATTCAAGATTGGATGAATAATAACCCAAGTTAATATTAACCCAATCTAAAACTGGAAAAAGGTTTAACAATAAGAATAACCCAAACTGAAAATAATCTCAAGAAAGCCAGTTAGTCTGAAGTGGAAATTTGACAAGAAACTCGGTCGCTCGCATTGTGGAGGCAAGTTTCCACTTCTAACTAACAGACTTTCTTGAGATTATTTTCAGTTTGGATTATTATTGATCTCTTTCCATTTTTGTCTTGGGTTATTAAACTTCAAAGTTTGAGTTGTCTTTAGATGGATGTCTCTTCTGTGTTTCCTTTTCTTTATAACTCAACAAGCAATCTCTTCTTGCAAGTCACAATAGGTGCTTCCAGTCATGATTGATGTTGGAACCAACAATGAAAAGCTTCGGAAGGACCCGTTATGTAAGTAATTCCACACTACTAGGAGGATAATAACTAAATTCTCTCTATAATTATTGTCTTAATGGAGGTTGATATCTATTTATATAAATCTACAATTTGAAGCCATGACTTATAGTATGTGTAAAATTTCCTTTTTATATTTGGTCAGGGTTCATACCTGTCCTTGGCTTATGTTTGAATTGGGATTAGGCATATGCGCAAAATGCTGTAGGGAAACTGTTGCATGGCTTCATGCATGTTTCGCGTTTTATCATCATACAAAATTCACTTGATACTTAATACTGTAATAAGATGATTTACCAAACCGTTTAAGAAAAAAGGATGTTATTCTTCAAAATGAGTTCGGCTACAGCTATTTCCATCTTATAATCCGTTAAATTTTTATATTGTTTTCACGATAAATTGACATCTTTAGAACATCCAAAAATTGCTCGATATTTTCTCTGGCTAGTTTCTCAACTTTCATGACTACTTCACATCTTTAAAACACCCAATTTCAATTTTAAAGTGACATCGAGATGATGCTTTCGTGCTTTAACTTGTGTTTATTGGCATAACATTTTTAATTAATCCATTTTTTGGTAGATTTAGGATTGCAACAACAACGATTGGATGGTGATGAATATTTGGCCATTATCGATGAATTCATGGAGGCCGTGTTTACTCGCTGGCCACATGTTATCGTTCAAGTAATTAAACTACTACTTTGGTCAAATAATTGGAATTTATTTTATTTTCTTATTTTTTATTGCATTTTTAAACTATGCAATAAATACTTAACTGTTGGAACAGTTTGAAGATTTCCAAAGCAAATGGGCATTTAAGTTATTGCAACGATACAGAAATACCTACAGAATGTTTAATGATGACGTTCAGGTTGACCATTCTCTACTTCTTCTACTTGATTTCTTTCCCTTTTCCCAATAGTTCTCCATGTTCATTGATTTCTTATACTATACTGATATGTAATTGAGTAAACGGAGTTTACTACATTTACTACAATTTAGAAGTTGAGATCAGAAGCGAGACCACGAAAGAAAGAAGTTGATTATCTAATTAATAAGTAGTTGATAAAGCTCTGATTCTCTTCTCTTTCAGTCTGAAGGCAGAGTTATGGGAAAAACTCCCACCGTACGGTCCCCACGCATGATTGCCTAGATTTATTTGACATTTTTCTTTCTTAATCTTTTAGCTATTTTTTTTTATTGGAAACAGATTTTTTTTCCCTTGAACTTTAGCTATTTAGATTATTATGTCTTCCATGTTCGCAATTTTATCAGAAAAGTTTCTGATGGCTGCTAAACGCACTTACTGGTTTATATGACAAGGATATATATATATATTTGATTCTGAAGGCACAGCATTGGTTGGGATAATTACAATGACATTTTATTTCTAAAAGCTATGTTTCGCTCAAAATCATGGGCCATGATCATCCAACACTAACAATTCAATGCCCAACTGTCTCTAGGGAACGGCAGGAGTTGCTATTGCTGGACTTTTAGGGGCTGTGAGAGCACAAGGAAGACCGATGATTGACTTTCCAAAGCAAAAGATTGTTGTTGCTGGTGCTGGAAGGTAATTGCATTCCTATTGGTGTTATCTAACATTAAAGATAGAAGGATAAAATTTCGAATTTTGTGATGATAGCTTTCATAGTGCCGCAAGCCTTGTTTCTTTTAGCTAAACATACAAAATTTCAACCATATAATCTTGCTATTCTGTTCTAACTCAATTTCTGTCAATTGTTCCATTTATTAAGAATTGACACTATTGCTTTCGTTTTTTTGTACATGTGTCGGAAATTAAGTTTTGGTCCCGTTTTTTTTTTTGAGAAACTTTCAATCATAATTTCATTGATATTGTGAAATTTCAAAAGGGCAAACATGCCAAAGAGATTACACGATGCTATTCATTTGTGCAATAAGGGATGTACCATCGTAATCACAAAAAAAAAAAAAGAAAAACTGGGTAAATTTACACCAAAACAGCATTCGAGGGAAGTTTTGGCTTATTATTTTTTTTAATGATGCAGCGCAGGGATTGGGGTTTTAAACGCGGCAAGGAAAACCATGGCGAGGATGTTGGGAAACAATGAAGCAGCATTCGAGGCTGCTCGTAGCCAATTTTGGGTGGTAGATGCCCAGGTGATGAACCGCCATTCATTCTTTGTATTTAAAATTCTCGAGCTAAAAGTTTTTTGATTTAAAAGCGAACAGAAAATTTATTTCAAGACTATTATGTTATGTCTTCTGTACTTAAAACCATGCCACTTTATGACTCTTTTGGGAGAACGAAAAATTTCCTTTACATCTTAAATTTTAATGGTCCTCTATACGTTTTTCTCCAACTTTTTTAACAAAAACAAAACATACGTATGTTTTGGTCCTCAATGTTTATATAAACCAAGAGTGCTCATTAGGATAAATGCTTTGGCCATGAGTTCGAAAACTTTTGGACCTATGTGGCACAATAAATATCTCACTAGTTTGTATCTCCTAAATTTTTACTCCTTTTTATTACATCAATGGGAAGTTATTTCTTGTAAAAAAGCAAAGAGTAGTTTTTCCTTATTTTGTATTATTTTGCTTATCTACATATTTCCAAGTTATGAGCTTACTTTTGTTACTGAGTTCAGCCAACATTTTCCCATGCATGAAGTGTGGTATCAAAGTTTTCGCTTATTTGTTAATTGTTTTGTTTTGTTGTTTTTTTTCCTCGTTGTCAGGGTTTGATCACAGAGGAACGAGAAAATATTGATCAAGATGCAAGTCCGTTTGCAAGGAAGGTCAAAGAAATTAATCGTCAAGGATTGAGGGAAGGTGCAAGTCTAGTGGAAGTGGTTTGTAAATTTCCCATTCTCCTCACTTATGGACTTATAGCTACTTTTACATAAAATAATTATGCCTTAGAACTCCTTTTGTTATGACGTTGACATTTGGAAATTGAAAGCTGTTGAAAAAGGTGGGATTTGATTGGAGAAAAATAGTTATTGGAATATAGTTCAATCTTGTGAGAGCGAGACATCGTATTTCATTGTCAGGATGTAAATGTACGAGTCAATTTAATACTGATCAAATGTTCTACGAGTGACCTGGTAGGGGTGCTTGGTGGTAAGAGGTTAGGATGTTTGAATTTACCCTCCAATGCTTTGGGTTCAATTCTCTTGGTTGAAAATTAAGGAGGTGTTCGGGGCAATAAGTGAGTTATTATGGTCCTATGTTATTATTATTTGATTCGGATAGTTTGTGTTTGAGGTGTAGATTATTTTAGTTTGGGTTATAATAGTATGTTTTTGGTGTGTAGATTATTTTAGTTTGATAAGGAAATAGTAAACACTATAGCAAAAATAAAAAAATGATGAATGTCAAATAGTAAAAACTGTAGCAATTGAGAGTTTTGAAATAGTGTTTACTGTAGTTAATTGGAGAGTTTTAAATAGTATTTACTATAGCAAGAGTTGGTTATTATAGTCTTAGATAATCATCTTTGCCCCAAATGACCTCTAAAGTTAGTGAATGTATTCATGCATTTTTTACACATTAAACTCAAGTGCATGGAAATTATTGAGTGTGATTTTAATCCATTAGGTTTGAAAATGTTGCAATGTTACTTCAAATGTTTGAATTTTGTTTAAATTTGGTCCCTAAGTTTCAAGATTTATACTTTTGATCTTGATTTTTCACTATTTGCTCACATTCAACCTTTAATATTAATGCCCATTAATTAATTTAAAAGAATTATAGTTGAGTTTCATTTTATAGTTATTTTTAATCAATTAATAGACATTAATGTCAAAATTGTAAGTGAGTATTTAGGGAAAAATGGATGCAAGTATAAATTTTGAAATATAGGGAACAAATTGAAACAATACTCCAAATTTAAGGACTAAAAGTGTAATATATTGAAACCTAGGGACTAAATTGAAATCAAACTCAAAATTTAAGGACTAAAAGTACAACATAATGAGGCCTAGGGACCACATATATACTAAAATCAAAATCTAAGGACCAAAAAAAGTATATTTTCCCTTCTTCTGTTATTTAAATATCACAGTTTATTTTTTATTTTTCGAAAATAAATGTCATATTTAATTCTATTGTCCCCATAGGAAACATATCTTGATTTTCTCCCAATTCCTTGGAAATTTCTATATTAGGTGCAACAAGTGAAACCTGATGTGCTTTTGGGATTGTCTGCCGTTGGTGGATTGTTCACAAAGGAGGTATTAAGCATGGATTTTTTTTAATGCAATATTATTTGTAGTCACTATTCACTTGGCTCTTTAATATTTTGTTTTTGGAGATAACAAACTGACTGTTCTTTCAGTTATTTGTGCTTTATGTATGTTGGATTTCTATTGATTCGGACACTATACCTTACTATGGATGGAGAAAGAGGGGAAAGGAAAATTGCAAAATTTCCATTCTATATCTTTTGCATTTTGCTCCATGCAAAAATGGGTCATATTCTATGGGAGCCTCTATGATGAGACAAAAAAAAAAAAAAAAAAAAAGAAAAGAAAAAGAAAGACGGCTGAAAACAGAAACAAAAGTCAGTGAGAAAATACAGAAATAAAAATGATGTAGACTTGGATAGAGATAAATGAATGAAAATGTAACTGAATTGTTTAGCTATCACCCAAATGTGAAACTTGTGCAATTTACTCTTCTCTTTTTATACACATGTGTTTAGGTATTAGAGGCTCTTAAAGGATCTACAGCTACAAGACCAGCTATTTTTGCCATGTCTAATCCTACAACAAATGGTACAGTTGTACAATCTATGAATTTCTCTTCCTGTGCGTGGGTCACAAATTTGTCCTTCAGTTGGTTGGTAATTCTTACATTATGTATTATCTATCTCATGTAGCCGAGTGCACTCCTGAAGAAGCATTCTCAATCTTGGGTGAAAACGTCATATTTGCAAGTGGAAGCCCATTCAAGGATGTTGATTTTGGTATGTGAAATAAATGCGTCCTGTGCTTTGATGCTTTTCACTTCTGTTTGATTTAAGATCTTAAAGTTAATACAAGGAAGAGGGATGCTTCTGCAAAAAAATAATAATCTCAAGAATATACTTCTCGGATCTTTCCATTTAAAGGTGTTGGAAAAGTAAGTAAAAAGTTTACTAGCAGCCAGCAAGAATACAGGAAATTTTGGATGCTTTCAGCCCAAAATTATGTTCTTGAATCTACAAGCGAGTGTGGGACGCATTTTTAATCGTCTACTTAGCTTGAACATCCTGTTTCAGATAACATCCGTTTTAAAAATCCCTAAGTAAGTTTTGCAGATTATTTGGACTTATATTTAACGACAGAGCTTTGAGTGTTTAGTGGGAATGAGAACATGTTTTTTTCTCAACAAAATTTGAGCAGCAAGTACCTCCTTCTTTCATTATGTATCCATAATCTGAGAAATTCTTTTTTAAATAGTTTCTTCTCAGTGTAATTTCTTTAAAAAAAAGTGAAAGCAAATCAAGGATTAGTTTAGTGCGTTATATTATCTTCTTTATAATTGCAATAATAATGTCAGTGGTAACTATAATTTGGTATTGTCCTTTCTTGATTTGACTTTTTTGTTTGTCGATCATGAAATTATTTTGCTTAGTTCCATGCTAGAGACAGTCTTTAATATAACCGATATTCTCCAGGAAATGGTCATATTGGCCACTGCAACCAAGGAAACAACATGTATCTTTTCCCAGGGTTAGTTTTCCAAGTTGCACCTCTAATGGCGATAAAGATAATATTTTATGATATTTGAGGTTTATAATATGTGCGGATGTATGCTTGTGCAGTATTGGGCTCGGTACACTTCTATCTGGTTCTCCTATCGTATCTGATGGCATGCTGCAGGCTGCTGCTGAGTGGTAAGGCTTCTACACTTTCAAGCTTATGTAGATTAAATTTTGTTGATGCAAATTTCAGAGGAAAAATGGCTAAAATTTTGGATGGATGGAATTTAATCATTTTGCAAAATTGCAGTGTAACGTTCTGCTGCTGCTAGGTGCAGTAGCTAGAGAAAACTCGTTGACTGGAAAAGGAATTTTATGTATTCTGCATTAAAAGAAAATTTTCACTGATTAGTGGGATCCACATAGAAATGCTCCCAAACTTTGTCTAAGAATGGAGTTAAAATAACATTCTTGAATGCGCATATATTTGAGGTAGAGTAATTACGATTCTTATAGAATACAAGAGCCCTTATTTATTTCTTACATAATTTGTCAGTTCTTTTTTAAGCGAATCCTTTCTTCCACACTAGATCTAATAGCATTTTGAGTCACAGAATTTTCAGCCTTGTACAAATGACACTGAGCACAAAACAAATTCAGTAGGAATATCTTCTTAACGTATAGGGAAAGATATTTTTGCTTTCCAATAGATCACCTCTCTGCCAAAGTCCATAGGGGAAAGGATTTTGTTGAAAACAAACTATTAACACGTTTTCAATTTTCTAAACACGAAGTGGACCACCATGGACAGATATTTCTCAAACTCCCTATGATGAGCGTTCAAATGAAAATTCACCTTTCTCATATACTTTTGTTTAACAACCCCATCAAATCGAGATATGTGCAAATTACCTTACTAATATTTGATTGGGAATATCAATCTCCACTTTCATAAAAGATTGTAAAATTTTATAAACACACACTTTAGGATTCTGAGATCTCAAAAAAGTGGAACTCTACTAAATGAAATTTTGATAGGAATTTTGAATTCTAAATCAAAAAGTTTATTTTTTAGTTCTACAGCATGTCAGGTATGAATTTTGAAGATGTAATTTCTTAATCAGCTGAACTATATTTAACTTGGAAAAGCAGTGTCATGCTAATTGAATCTTCCAAATTTTGATCTCCACTATTCTCTATACTTGGCAACTAAGGACTTGCATTTAGTTGTGAAACCAATGGACTACTTGAGCTGGTATAGTTGCACTGGGATGTAAATCTCCATCTTTCGTCTATTGCCATGTTTTTCATGTGAAGTTCCATTACATGTGGGAACCCTTAAGAATCAAGATCCTAGGTGATCCACTGTTTGCATAGTACCTTACTTTCTTAGGGAAGCTTCTTCTGAGATGTTCTAAATCTTTTCGGAGTTGTTACATTCTTTGCGTCTGCTCCTTCATCCACAGGAATGTCTCTTATTTGTGTATTTTAATGGAAAATCTTCCAACTCCCATGGAAATTGAGGATCCAAGCGAATAAGAGATTCAAGTGAGGGATACTAGTTCAAGTGAGAACACGGAACCCTGAAGAGAGAACTTTGCATGAGCTTATTTGCTAAAAGCAATTTTGACATTGATATTTTTTTGTTTCGCCTATTGATAACTTCATAAGAACCAAATGGCCTAGGGGGGCTAGGATTTTATGAACGTATCCAAAGATCTAATATAGGAACTTGGTTTTTGTTAGTGGGAGATGAAAGATGCAAATGCCTATTGAGATTCGAGAACTTCCCATTTTCTTTATCATTTTGAAACCGTTTTATTCCTTCAACTCTCGGATAGGATTGGATATGGTTGCACCTTTTCACATTTTGGTGTGTGTGTGCATGAGGCATTTATCATGTTTACTGGCAGTGGAGAGTTGAGTAAACCATGTAAACTCAGCAACTGAATGGCAATATCAGCCAATTGAGAGTTTCATGTTTATGCAATTAAAATAATTTTAGAAGAAAGATGTATTTATGAAATGAACTTAGGTTTAATTTAATAGTTTATTCAACTTCTACTCATTTTCTAATTTAGAGCATGCTCGGTACACATCATACTACATAACAATGTATGAAGAATACCAACTACAAGATATAATCTTCTGAACTTTCTCATTAAATTAATAACATAACAGCTTCTTACATTACAATAATGATTATTTTGTCATTCCATTTGTAACAAAACAGCCTGGCTGCATATATGACTGAAGATGAAGTTCACGAAGGCATTATTTATCCATCAATTTCTAGGTAAAGTCATCTTTATTTGATGTGTAAATATTTCTTTTTGATTTCATTGAGATAAAAAAGTGAGAAGTTTCATTACCGTGTTTTCGTTTGTAGCATCCGTGATATTACAAAGGAAATTGCAGCAGCTGTTATTATGGAAGCCATAGAAGAGGATCTTGTAGAAGGATACCGCGAGGTCGATGCTCGAGAACTCCGAAAATTTAGCAAGGTAGATTCTATTATTAGAAATCTTACTAATCCTCTTTCGCTTGGTTTTAAAATTTTGTCCTTGGACTGCTCAACAACACAATCTGAACTTACATAAATTGATTTACATTTATTCTTGAATAAGAGGTCAGAAAATTAATTCTATCACACTATATTTTGAACTAAAAAAAGAAAACCATTTTGTACTAGAGGATTGAACCATCGACCTATAGAATGATATTTGTTATCTTATGATTGAGCTATAATGAGTTGGTGAAGGGTCTAATGACTTAGTTCGGCTGTATGATCTTTATAATATAATGCGAATTTTAAGTCAATAACATCACCCGTGCATAATCTGATTTCATGTTTTTTTTTTCAGGAGGAAATTTTGGAATTTGTGAAGAACAACATGTGGAGTCCAGAATACCCAACACTAGTTTACAATCAGGATTAATCTACCTTTGTAAGAACAAATCAATACTATCATTTCATGTTTATGTTGATGCACGTTCAATTTTGCCTTAGAATTTGGAACTTCTAAATTCTAATGCATGGGTAGCCATAAAGATCAAAGTTGTAAATTCATGATCCATATTTTACCATTTTTTTTTTGGAGTTACTACATTTTGATTCTTCTCCCACGTTATATATGGTTGCATTGACATTCAT

mRNA sequence

TTTACACCTGTCCCCTCCATCTCCTTGGTTCTCAGTCTCGCCCAATCTCACTCCGAGAAGCTGCTCTGCTCTGAATCTCTCCAGTTTTCGTGGAGAAGCCATTGAAATCAGCTAAATGACGAAGTGGAATTCACTCTCTACTTCGCTTATGAAGCGTCTCAAGCTTCACTACCAGATGACCAACAGTATTCCAGCCTTAGCTCAGGCCAGATCTTTCACCACAAGCGAAGGACATCGTCCCACTATTGTTCATAAACGTAGCCTTGATATTCTTCACGATCCGTGGTTTAACAAAGGAACTGCCTTTACAATGACAGAACGTGACAGGCTTGATCTTCGAGGACTTCTTCCACCAAATGTAATGTCTTCGGAGCAGCAAATTGAACGATTTATGTTTGACCTGAAGAGGCTTGAAGTGCAAGCAAGAGATGGGCCTTCAGATCCAAACGCTTTGGCCAAATGGCGAATTCTGAACAGATTACACGACAGAAATGAAACAATGTACTATAAAGTTTTGATTGCTCACATTGAGGAGTATGCGCCCATAGTATACACGCCCACTGTAGGTCTCGTTTGCCAAAACTATAGTGGCTTGTTTAGAAGGCCAAGGGGAATGTATTTCAGTGCAGAAGATCGTGGAGAAATGATGTCTATGGTTTATAATTGGCCTGCTGATCAGGTTGATATGATTGTTGTCACCGATGGAAGTAGAATATTAGGACTTGGAGATCTTGGAGTTCATGGGATTGGGATTGCCATAGGAAAGTTGGATTTATATGTTGCTGCTGCCGGGATAAATCCTCAGAGGGTGCTTCCAGTCATGATTGATGTTGGAACCAACAATGAAAAGCTTCGGAAGGACCCGTTATATTTAGGATTGCAACAACAACGATTGGATGGTGATGAATATTTGGCCATTATCGATGAATTCATGGAGGCCGTGTTTACTCGCTGGCCACATGTTATCGTTCAATTTGAAGATTTCCAAAGCAAATGGGCATTTAAGTTATTGCAACGATACAGAAATACCTACAGAATGTTTAATGATGACGTTCAGGGAACGGCAGGAGTTGCTATTGCTGGACTTTTAGGGGCTGTGAGAGCACAAGGAAGACCGATGATTGACTTTCCAAAGCAAAAGATTGTTGTTGCTGGTGCTGGAAGCGCAGGGATTGGGGTTTTAAACGCGGCAAGGAAAACCATGGCGAGGATGTTGGGAAACAATGAAGCAGCATTCGAGGCTGCTCGTAGCCAATTTTGGGTGGTAGATGCCCAGGGTTTGATCACAGAGGAACGAGAAAATATTGATCAAGATGCAAGTCCGTTTGCAAGGAAGGTCAAAGAAATTAATCGTCAAGGATTGAGGGAAGGTGCAAGTCTAGTGGAAGTGGTGCAACAAGTGAAACCTGATGTGCTTTTGGGATTGTCTGCCGTTGGTGGATTGTTCACAAAGGAGGTATTAGAGGCTCTTAAAGGATCTACAGCTACAAGACCAGCTATTTTTGCCATGTCTAATCCTACAACAAATGCCGAGTGCACTCCTGAAGAAGCATTCTCAATCTTGGGTGAAAACGTCATATTTGCAAGTGGAAGCCCATTCAAGGATGTTGATTTTGGAAATGGTCATATTGGCCACTGCAACCAAGGAAACAACATGTATCTTTTCCCAGGTATTGGGCTCGGTACACTTCTATCTGGTTCTCCTATCGTATCTGATGGCATGCTGCAGGCTGCTGCTGAGTGCCTGGCTGCATATATGACTGAAGATGAAGTTCACGAAGGCATTATTTATCCATCAATTTCTAGCATCCGTGATATTACAAAGGAAATTGCAGCAGCTGTTATTATGGAAGCCATAGAAGAGGATCTTGTAGAAGGATACCGCGAGGTCGATGCTCGAGAACTCCGAAAATTTAGCAAGGAGGAAATTTTGGAATTTGTGAAGAACAACATGTGGAGTCCAGAATACCCAACACTAGTTTACAATCAGGATTAATCTACCTTTGTAAGAACAAATCAATACTATCATTTCATGTTTATGTTGATGCACGTTCAATTTTGCCTTAGAATTTGGAACTTCTAAATTCTAATGCATGGGTAGCCATAAAGATCAAAGTTGTAAATTCATGATCCATATTTTACCATTTTTTTTTTGGAGTTACTACATTTTGATTCTTCTCCCACGTTATATATGGTTGCATTGACATTCAT

Coding sequence (CDS)

ATGACGAAGTGGAATTCACTCTCTACTTCGCTTATGAAGCGTCTCAAGCTTCACTACCAGATGACCAACAGTATTCCAGCCTTAGCTCAGGCCAGATCTTTCACCACAAGCGAAGGACATCGTCCCACTATTGTTCATAAACGTAGCCTTGATATTCTTCACGATCCGTGGTTTAACAAAGGAACTGCCTTTACAATGACAGAACGTGACAGGCTTGATCTTCGAGGACTTCTTCCACCAAATGTAATGTCTTCGGAGCAGCAAATTGAACGATTTATGTTTGACCTGAAGAGGCTTGAAGTGCAAGCAAGAGATGGGCCTTCAGATCCAAACGCTTTGGCCAAATGGCGAATTCTGAACAGATTACACGACAGAAATGAAACAATGTACTATAAAGTTTTGATTGCTCACATTGAGGAGTATGCGCCCATAGTATACACGCCCACTGTAGGTCTCGTTTGCCAAAACTATAGTGGCTTGTTTAGAAGGCCAAGGGGAATGTATTTCAGTGCAGAAGATCGTGGAGAAATGATGTCTATGGTTTATAATTGGCCTGCTGATCAGGTTGATATGATTGTTGTCACCGATGGAAGTAGAATATTAGGACTTGGAGATCTTGGAGTTCATGGGATTGGGATTGCCATAGGAAAGTTGGATTTATATGTTGCTGCTGCCGGGATAAATCCTCAGAGGGTGCTTCCAGTCATGATTGATGTTGGAACCAACAATGAAAAGCTTCGGAAGGACCCGTTATATTTAGGATTGCAACAACAACGATTGGATGGTGATGAATATTTGGCCATTATCGATGAATTCATGGAGGCCGTGTTTACTCGCTGGCCACATGTTATCGTTCAATTTGAAGATTTCCAAAGCAAATGGGCATTTAAGTTATTGCAACGATACAGAAATACCTACAGAATGTTTAATGATGACGTTCAGGGAACGGCAGGAGTTGCTATTGCTGGACTTTTAGGGGCTGTGAGAGCACAAGGAAGACCGATGATTGACTTTCCAAAGCAAAAGATTGTTGTTGCTGGTGCTGGAAGCGCAGGGATTGGGGTTTTAAACGCGGCAAGGAAAACCATGGCGAGGATGTTGGGAAACAATGAAGCAGCATTCGAGGCTGCTCGTAGCCAATTTTGGGTGGTAGATGCCCAGGGTTTGATCACAGAGGAACGAGAAAATATTGATCAAGATGCAAGTCCGTTTGCAAGGAAGGTCAAAGAAATTAATCGTCAAGGATTGAGGGAAGGTGCAAGTCTAGTGGAAGTGGTGCAACAAGTGAAACCTGATGTGCTTTTGGGATTGTCTGCCGTTGGTGGATTGTTCACAAAGGAGGTATTAGAGGCTCTTAAAGGATCTACAGCTACAAGACCAGCTATTTTTGCCATGTCTAATCCTACAACAAATGCCGAGTGCACTCCTGAAGAAGCATTCTCAATCTTGGGTGAAAACGTCATATTTGCAAGTGGAAGCCCATTCAAGGATGTTGATTTTGGAAATGGTCATATTGGCCACTGCAACCAAGGAAACAACATGTATCTTTTCCCAGGTATTGGGCTCGGTACACTTCTATCTGGTTCTCCTATCGTATCTGATGGCATGCTGCAGGCTGCTGCTGAGTGCCTGGCTGCATATATGACTGAAGATGAAGTTCACGAAGGCATTATTTATCCATCAATTTCTAGCATCCGTGATATTACAAAGGAAATTGCAGCAGCTGTTATTATGGAAGCCATAGAAGAGGATCTTGTAGAAGGATACCGCGAGGTCGATGCTCGAGAACTCCGAAAATTTAGCAAGGAGGAAATTTTGGAATTTGTGAAGAACAACATGTGGAGTCCAGAATACCCAACACTAGTTTACAATCAGGATTAA

Protein sequence

MTKWNSLSTSLMKRLKLHYQMTNSIPALAQARSFTTSEGHRPTIVHKRSLDILHDPWFNKGTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMFDLKRLEVQARDGPSDPNALAKWRILNRLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSMVYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVGTNNEKLRKDPLYLGLQQQRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQRYRNTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAARKTMARMLGNNEAAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGASLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPEEAFSILGENVIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAAAECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYREVDARELRKFSKEEILEFVKNNMWSPEYPTLVYNQD

Homology

[Close]

BLAST of PI0000230 vs. ExPASy Swiss-Prot
Match: Q9SIU0 (NAD-dependent malic enzyme 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NAD-ME1 PE=1 SV=1)

HSP 1 Score: 1067.8 bits (2760), Expect = 4.8e-311
Identity = 530/620 (85.48%), Postives = 579/620 (93.39%), Query Frame = 0


Query: 7   LSTSLMKRLKLHYQMTNSIPALAQARSFTTSEGHRPTIVHKRSLDILHDPWFNKGTAFTM 66
           LS+S + R+ LH ++  S    +  RSFTTSEGHRPTIVHK+ LDILHDPWFNKGTAFTM
Sbjct: 9   LSSSSLSRI-LHRRILYS----SAVRSFTTSEGHRPTIVHKQGLDILHDPWFNKGTAFTM 68

Query: 67  TERDRLDLRGLLPPNVMSSEQQIERFMFDLKRLEVQARDGPSDPNALAKWRILNRLHDRN 126
           TER+RLDLRGLLPPNVM SEQQI RFM DLKRLE QARDGPSDPNALAKWRILNRLHDRN
Sbjct: 69  TERNRLDLRGLLPPNVMDSEQQIFRFMTDLKRLEEQARDGPSDPNALAKWRILNRLHDRN 128

Query: 127 ETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSMVYNWPA 186
           ETMYYKVLI +IEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSMVYNWPA
Sbjct: 129 ETMYYKVLINNIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSMVYNWPA 188

Query: 187 DQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVGTNNEKL 246
           +QVDMIVVTDGSRILGLGDLGVHGIGIA+GKLDLYVAAAGINPQRVLPVMIDVGTNNEKL
Sbjct: 189 EQVDMIVVTDGSRILGLGDLGVHGIGIAVGKLDLYVAAAGINPQRVLPVMIDVGTNNEKL 248

Query: 247 RKDPLYLGLQQQRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQRYRNTY 306
           R DP+YLGLQQ+RL+ D+Y+ +IDEFMEAV+TRWPHVIVQFEDFQSKWAFKLLQRYR TY
Sbjct: 249 RNDPMYLGLQQRRLEDDDYIDVIDEFMEAVYTRWPHVIVQFEDFQSKWAFKLLQRYRCTY 308

Query: 307 RMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAARKTMARM 366
           RMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPK KIVVAGAGSAGIGVLNAARKTMARM
Sbjct: 309 RMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKMKIVVAGAGSAGIGVLNAARKTMARM 368

Query: 367 LGNNEAAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGASLVEVV 426
           LGN E AF++A+SQFWVVDAQGLITE RENID +A PFARK KE+ RQGL+EGA+LVEVV
Sbjct: 369 LGNTETAFDSAQSQFWVVDAQGLITEGRENIDPEAQPFARKTKEMERQGLKEGATLVEVV 428

Query: 427 QQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPEEAFSILGEN 486
           ++VKPDVLLGLSAVGGLF+KEVLEA+KGST+TRPAIFAMSNPT NAECTP++AFSILGEN
Sbjct: 429 REVKPDVLLGLSAVGGLFSKEVLEAMKGSTSTRPAIFAMSNPTKNAECTPQDAFSILGEN 488

Query: 487 VIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAAAECLAA 546
           +IFASGSPFK+V+FGNGH+GHCNQGNNMYLFPGIGLGTLLSG+PIVSDGMLQAA+ECLAA
Sbjct: 489 MIFASGSPFKNVEFGNGHVGHCNQGNNMYLFPGIGLGTLLSGAPIVSDGMLQAASECLAA 548

Query: 547 YMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYREVDARELRKFSKEEIL 606
           YM+E+EV EGIIYP IS IRDITK IAAAVI EAIEEDLVEGYRE+DARE++K  +E ++
Sbjct: 549 YMSEEEVLEGIIYPPISRIRDITKRIAAAVIKEAIEEDLVEGYREMDAREIQKLDEEGLM 608

Query: 607 EFVKNNMWSPEYPTLVYNQD 627
           E+V+NNMW+PEYPTLVY  D
Sbjct: 609 EYVENNMWNPEYPTLVYKDD 623

[Close]

BLAST of PI0000230 vs. ExPASy Swiss-Prot
Match: P37221 (NAD-dependent malic enzyme 62 kDa isoform, mitochondrial OS=Solanum tuberosum OX=4113 PE=1 SV=1)

HSP 1 Score: 1055.8 bits (2729), Expect = 1.9e-307
Identity = 524/620 (84.52%), Postives = 571/620 (92.10%), Query Frame = 0


Query: 7   LSTSLMKRLKLHYQMTNSIPALAQARSFTTSEGHRPTIVHKRSLDILHDPWFNKGTAFTM 66
           LS++L+KR  LH ++  ++ + + +R+FTT+EGHRPTIVHKRSLDILHDPWFNKGTAF+ 
Sbjct: 10  LSSTLLKR--LHQRVAAAVNS-SSSRNFTTTEGHRPTIVHKRSLDILHDPWFNKGTAFSF 69

Query: 67  TERDRLDLRGLLPPNVMSSEQQIERFMFDLKRLEVQARDGPSDPNALAKWRILNRLHDRN 126
           TERDRL +RGLLPPNVMS EQQI RFM DLKRLEVQARDGPSDP  LAKWRILNRLHDRN
Sbjct: 70  TERDRLHIRGLLPPNVMSFEQQIARFMADLKRLEVQARDGPSDPYVLAKWRILNRLHDRN 129

Query: 127 ETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSMVYNWPA 186
           ET+YYKVL+ +IEEYAPIVYTPTVGLVCQ YSGLFRRPRGMYFSAEDRGEMMSMVYNWPA
Sbjct: 130 ETLYYKVLMENIEEYAPIVYTPTVGLVCQKYSGLFRRPRGMYFSAEDRGEMMSMVYNWPA 189

Query: 187 DQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVGTNNEKL 246
           DQVDMIVVTDGSRILGLGDLG+ GIGIAIGKLDLYVAAAGINPQRVLPVMIDVGT+NE L
Sbjct: 190 DQVDMIVVTDGSRILGLGDLGIQGIGIAIGKLDLYVAAAGINPQRVLPVMIDVGTDNENL 249

Query: 247 RKDPLYLGLQQQRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQRYRNTY 306
            KDPLYLGLQ  RLDG+EY+ +IDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQRYRN Y
Sbjct: 250 LKDPLYLGLQDHRLDGEEYIEVIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQRYRNNY 309

Query: 307 RMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAARKTMARM 366
           RMFNDD+QGTAGVAIAGLLGAVRAQGRPMIDFPK KIVVAGAGSAGIGVLNAARKTMARM
Sbjct: 310 RMFNDDIQGTAGVAIAGLLGAVRAQGRPMIDFPKMKIVVAGAGSAGIGVLNAARKTMARM 369

Query: 367 LGNNEAAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGASLVEVV 426
           LGN E AFE+ARSQFWVVDA+GLITE REN+D DA PFARK+KEI RQGL EGA+L EVV
Sbjct: 370 LGNTEIAFESARSQFWVVDAKGLITEARENVDPDARPFARKIKEIERQGLSEGATLAEVV 429

Query: 427 QQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPEEAFSILGEN 486
           ++VKPDVLLGLSA GGLF+KEVLEALK ST+TRPAIF MSNPT NAECTPEEAFSILGEN
Sbjct: 430 REVKPDVLLGLSACGGLFSKEVLEALKHSTSTRPAIFPMSNPTRNAECTPEEAFSILGEN 489

Query: 487 VIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAAAECLAA 546
           +IFASGSPFKDVD GNGH+GHCNQ NNM+LFPGIGLGTLLSGS IVSDGMLQAAAECLAA
Sbjct: 490 IIFASGSPFKDVDLGNGHVGHCNQANNMFLFPGIGLGTLLSGSRIVSDGMLQAAAECLAA 549

Query: 547 YMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYREVDARELRKFSKEEIL 606
           Y+TE+EV +GIIYPSIS IRDITKE+AAAV+ EAIEEDL EGYRE+D+RELRK  + +I 
Sbjct: 550 YITEEEVLKGIIYPSISRIRDITKEVAAAVVKEAIEEDLAEGYREMDSRELRKLDEAQIS 609

Query: 607 EFVKNNMWSPEYPTLVYNQD 627
           EFV+NNMWSP+YPTLVY +D
Sbjct: 610 EFVENNMWSPDYPTLVYKKD 626

[Close]

BLAST of PI0000230 vs. ExPASy Swiss-Prot
Match: P37224 (NAD-dependent malic enzyme 65 kDa isoform, mitochondrial OS=Amaranthus hypochondriacus OX=28502 PE=1 SV=1)

HSP 1 Score: 974.5 bits (2518), Expect = 5.5e-283
Identity = 485/619 (78.35%), Postives = 553/619 (89.34%), Query Frame = 0


Query: 7   LSTSLMKRLKLHYQMTNSIPALAQARSFTTSEGHRPTIVHKRSLDILHDPWFNKGTAFTM 66
           L++SL++RLK      + I  ++  RSF TSEGHR  IV+KRSLDIL DPWFNKGTAF+M
Sbjct: 10  LTSSLIRRLK------DQIANVSNHRSFATSEGHRLAIVNKRSLDILQDPWFNKGTAFSM 69

Query: 67  TERDRLDLRGLLPPNVMSSEQQIERFMFDLKRLEVQARDGPSDPNALAKWRILNRLHDRN 126
           TERDRLDLRGLLPPNVM++EQQIERF  DL+ LE+  +DGPSD   LAKWRILNRLHDRN
Sbjct: 70  TERDRLDLRGLLPPNVMTTEQQIERFTADLRVLELTTKDGPSDTYDLAKWRILNRLHDRN 129

Query: 127 ETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSMVYNWPA 186
           ETM++KVLI +IEEYAPIV TPTVGLVCQ +SGL+RRPRGMYFS++DRGEMMSMVYNWPA
Sbjct: 130 ETMFFKVLIENIEEYAPIVSTPTVGLVCQKFSGLYRRPRGMYFSSDDRGEMMSMVYNWPA 189

Query: 187 DQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVGTNNEKL 246
           +QVDMIVVTDGSRILGLGDLGVHGIG+AIGKLDLYVAAAGINPQRVLPVMIDVGTNNE L
Sbjct: 190 EQVDMIVVTDGSRILGLGDLGVHGIGVAIGKLDLYVAAAGINPQRVLPVMIDVGTNNEDL 249

Query: 247 RKDPLYLGLQQQRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQRYRNTY 306
            K+PLYLGLQ++RLDG+EYLA++DEFMEAVFTRWP+VIVQFED Q+KWA  LLQRYR+ Y
Sbjct: 250 LKNPLYLGLQKKRLDGEEYLAVMDEFMEAVFTRWPNVIVQFEDIQNKWALTLLQRYRHKY 309

Query: 307 RMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAARKTMARM 366
           R FN DVQGT+GVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGS+G+GVLNAARKTMARM
Sbjct: 310 RTFNVDVQGTSGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSSGVGVLNAARKTMARM 369

Query: 367 LGNNEAAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGASLVEVV 426
           LGN+E+AF+ ARSQFWVVD +GLITE+R N+D +  PFA K  EI+ QGL EGA LVEVV
Sbjct: 370 LGNDESAFDRARSQFWVVDDKGLITEKRANLDPEVQPFAWKENEISLQGLNEGAKLVEVV 429

Query: 427 QQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPEEAFSILGEN 486
           +QVKPDVLLGLSA GGLF+KEVLEALK ST+TRPAIFAMSNPT NAECTPEEAFSI+G++
Sbjct: 430 RQVKPDVLLGLSAYGGLFSKEVLEALKDSTSTRPAIFAMSNPTKNAECTPEEAFSIVGDH 489

Query: 487 VIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAAAECLAA 546
           V++ASGSPFKDVD GNG IGH NQGNNMYLFPGIGLG LLSGS I+SD M QAAAE LA 
Sbjct: 490 VVYASGSPFKDVDLGNGKIGHVNQGNNMYLFPGIGLGVLLSGSRIISDSMFQAAAERLAG 549

Query: 547 YMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYREVDARELRKFSKEEIL 606
           YMT++EV  G+IYPSIS IRDITKE+AAAVI EA+EEDL EGYR++DAREL+K ++E+IL
Sbjct: 550 YMTDEEVINGVIYPSISRIRDITKEVAAAVIKEAVEEDLAEGYRDMDARELQKLNEEQIL 609

Query: 607 EFVKNNMWSPEYPTLVYNQ 626
           E+++ NMW+PEYPTLVY +
Sbjct: 610 EYIEKNMWNPEYPTLVYKK 622

[Close]

BLAST of PI0000230 vs. ExPASy Swiss-Prot
Match: P37225 (NAD-dependent malic enzyme 59 kDa isoform, mitochondrial OS=Solanum tuberosum OX=4113 PE=1 SV=1)

HSP 1 Score: 793.9 bits (2049), Expect = 1.3e-228
Identity = 389/582 (66.84%), Postives = 468/582 (80.41%), Query Frame = 0


Query: 42  PTIVHKRSLDILHDPWFNKGTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMFDLKRLEV 101
           P IVHKR  DILHDPWFNK T F MTERDRL LRGLLPP V+S EQQ +RFM   + LE 
Sbjct: 25  PCIVHKRGADILHDPWFNKDTGFPMTERDRLGLRGLLPPRVISFEQQYDRFMESFRSLEK 84

Query: 102 QARDGPSDPNALAKWRILNRLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLF 161
                P    +LAKWRILNRLHDRNET+YY+VLI +I+++API+YTPTVGLVCQNYSGLF
Sbjct: 85  NTEGQPDSVVSLAKWRILNRLHDRNETLYYRVLIDNIKDFAPIIYTPTVGLVCQNYSGLF 144

Query: 162 RRPRGMYFSAEDRGEMMSMVYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLY 221
           RRPRGMYFSA+D+GEMMSM++NWP+ QVDMIV+TDGSRILGLGDLGV GIGI IGKLD+Y
Sbjct: 145 RRPRGMYFSAKDKGEMMSMIFNWPSTQVDMIVLTDGSRILGLGDLGVQGIGIPIGKLDMY 204

Query: 222 VAAAGINPQRVLPVMIDVGTNNEKLRKDPLYLGLQQQRLDGDEYLAIIDEFMEAVFTRWP 281
           VAAAGINPQRVLPVM+DVGTNN+KL +DPLYLGL+Q RL+G+EYL+I+DEF+EAV  RWP
Sbjct: 205 VAAAGINPQRVLPVMLDVGTNNQKLLEDPLYLGLRQPRLEGEEYLSIVDEFVEAVHARWP 264

Query: 282 HVIVQFEDFQSKWAFKLLQRYRNTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQ 341
             +VQFEDFQ+KWAF+ L RYR  + MFNDD+QGTAGVA+AGLLG VRAQGRP+ DF  Q
Sbjct: 265 KAVVQFEDFQAKWAFETLDRYRKKFCMFNDDIQGTAGVALAGLLGTVRAQGRPLTDFANQ 324

Query: 342 KIVVAGAGSAGIGVLNAARKTMARMLGNNEAAFEAARSQFWVVDAQGLITEERENIDQDA 401
           KIVV GAGSAG+GVL  A + ++RM G       +A   F+++D  GLIT++R++ID  A
Sbjct: 325 KIVVVGAGSAGLGVLKMALQAVSRMTG------PSADPHFFLLDKNGLITKDRKDIDPAA 384

Query: 402 SPFARKVKEINRQGLREGASLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPA 461
            PFA+   EI   GL+EGA L EVV++VKP VLLGLS VGG+F +EVL A+K S + RPA
Sbjct: 385 LPFAKAHHEIEGLGLQEGAGLAEVVKKVKPHVLLGLSGVGGIFHEEVLRAMKESDSVRPA 444

Query: 462 IFAMSNPTTNAECTPEEAFSILGENVIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIG 521
           IFAMSNPT NAEC P +AF + GE+++FASGSPF +VD GNG IGH NQ NNMYLFPGIG
Sbjct: 445 IFAMSNPTNNAECCPVDAFKLAGEDIVFASGSPFANVDLGNGKIGHVNQANNMYLFPGIG 504

Query: 522 LGTLLSGSPIVSDGMLQAAAECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAI 581
           LG LLSG+  +SD ML+AAAECLA+YM++DE++ GI+YPSI  IRDIT E+ AAV+  A+
Sbjct: 505 LGALLSGARNISDTMLEAAAECLASYMSDDEINRGILYPSIDDIRDITAEVGAAVLRAAV 564

Query: 582 EEDLVEGYREVDARELRKFSKEEILEFVKNNMWSPEYPTLVY 624
            EDL EG+ +V  +EL+  SKEE +E V+ NMW P Y  LV+
Sbjct: 565 AEDLAEGHGDVGVKELQHMSKEETIEHVRQNMWYPVYGPLVH 600

[Close]

BLAST of PI0000230 vs. ExPASy Swiss-Prot
Match: Q8L7K9 (NAD-dependent malic enzyme 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NAD-ME2 PE=1 SV=1)

HSP 1 Score: 773.5 bits (1996), Expect = 1.9e-222
Identity = 383/614 (62.38%), Postives = 478/614 (77.85%), Query Frame = 0


Query: 21  MTNSIPALAQARSFTTSEGHR---------PTIVHKRSLDILHDPWFNKGTAFTMTERDR 80
           M  +I  L++A +   + G R         P IVHKR  DILHDPWFNK T F +TERDR
Sbjct: 2   MWKNIAGLSKAAAAARTHGSRRCFSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDR 61

Query: 81  LDLRGLLPPNVMSSEQQIERFMFDLKRLEVQARDGPSDPNALAKWRILNRLHDRNETMYY 140
           L +RGLLPP VM+  QQ +RF+   + LE   +  P +  ALAKWR+LNRLHDRNET+YY
Sbjct: 62  LGIRGLLPPRVMTCVQQCDRFIESFRSLENNTKGEPENVVALAKWRMLNRLHDRNETLYY 121

Query: 141 KVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSMVYNWPADQVDM 200
           +VLI +I+++API+YTPTVGLVCQNYSGL+RRPRGMYFSA+D+GEMMSM+YNWPA QVDM
Sbjct: 122 RVLIDNIKDFAPIIYTPTVGLVCQNYSGLYRRPRGMYFSAKDKGEMMSMIYNWPAPQVDM 181

Query: 201 IVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVGTNNEKLRKDPL 260
           IV+TDGSRILGLGDLGV GIGI IGKLD+YVAAAGINPQRVLP+M+DVGTNNEKL ++ L
Sbjct: 182 IVITDGSRILGLGDLGVQGIGIPIGKLDMYVAAAGINPQRVLPIMLDVGTNNEKLLQNDL 241

Query: 261 YLGLQQQRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQRYRNTYRMFND 320
           YLG++Q RL+G+EYL IIDEFMEA FTRWP  +VQFEDFQ+KWAF  L+RYR  + MFND
Sbjct: 242 YLGVRQPRLEGEEYLEIIDEFMEAAFTRWPKAVVQFEDFQAKWAFGTLERYRKKFCMFND 301

Query: 321 DVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAARKTMARMLGNNE 380
           DVQGTAGVA+AGLLG VRAQGRP+ DF  QKIVV GAGSAG+GV   A + +ARM G +E
Sbjct: 302 DVQGTAGVALAGLLGTVRAQGRPISDFVNQKIVVVGAGSAGLGVTKMAVQAVARMAGISE 361

Query: 381 AAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGASLVEVVQQVKP 440
           +    A   F+++D  GL+T ER  +D  A  FA+   EI     REGAS+VEVV++V+P
Sbjct: 362 S---EATKNFYLIDKDGLVTTERTKLDPGAVLFAKNPAEI-----REGASIVEVVKKVRP 421

Query: 441 DVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPEEAFSILGENVIFAS 500
            VLLGLS VGG+F +EVL+A++ S + +PAIFAMSNPT NAECT  +AF   G N++FAS
Sbjct: 422 HVLLGLSGVGGIFNEEVLKAMRESDSCKPAIFAMSNPTLNAECTAADAFKHAGGNIVFAS 481

Query: 501 GSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAAAECLAAYMTED 560
           GSPF++V+  NG +GH NQ NNMYLFPGIGLGTLLSG+ IV+DGMLQAA+ECLA+YMT++
Sbjct: 482 GSPFENVELENGKVGHVNQANNMYLFPGIGLGTLLSGARIVTDGMLQAASECLASYMTDE 541

Query: 561 EVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYREVDARELRKFSKEEILEFVKN 620
           EV +GI+YPSI++IR IT E+ AAV+  A+ +D+ EG+ +V  ++L   SKE+ + ++  
Sbjct: 542 EVQKGILYPSINNIRHITAEVGAAVLRAAVTDDIAEGHGDVGPKDLSHMSKEDTVNYITR 601

Query: 621 NMWSPEYPTLVYNQ 626
           NMW P Y  LV+ +
Sbjct: 602 NMWFPVYSPLVHEK 607

[Close]

BLAST of PI0000230 vs. ExPASy TrEMBL
Match: A0A1S3AVM9 (Malic enzyme OS=Cucumis melo OX=3656 GN=LOC103483428 PE=3 SV=1)

HSP 1 Score: 1228.8 bits (3178), Expect = 0.0e+00
Identity = 618/626 (98.72%), Postives = 621/626 (99.20%), Query Frame = 0


Query: 1   MTKWNSLSTSLMKRLKLHYQMTNSIPALAQARSFTTSEGHRPTIVHKRSLDILHDPWFNK 60
           MTKWNSLS SLMKRLKLHYQMTNSIPALAQ+RSFTTSEGHRPTIVHKRSLDILHDPWFNK
Sbjct: 1   MTKWNSLSASLMKRLKLHYQMTNSIPALAQSRSFTTSEGHRPTIVHKRSLDILHDPWFNK 60

Query: 61  GTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMFDLKRLEVQARDGPSDPNALAKWRILN 120
           GTAFTMTERDRLDLRGLLPPNVMSSEQQIERFM DLKRLEVQARDGPSDPNALAKWRILN
Sbjct: 61  GTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPNALAKWRILN 120

Query: 121 RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSM 180
           RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSM
Sbjct: 121 RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSM 180

Query: 181 VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG 240
           VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG
Sbjct: 181 VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG 240

Query: 241 TNNEKLRKDPLYLGLQQQRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ 300
           TNNEKL KDPLYLGLQQ RLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ
Sbjct: 241 TNNEKLLKDPLYLGLQQHRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ 300

Query: 301 RYRNTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR 360
           RYRNTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR
Sbjct: 301 RYRNTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR 360

Query: 361 KTMARMLGNNEAAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGA 420
           KTMARMLGNNEAAFEAARSQFWVVDAQGLITEEREN+DQDASPFARKVKEINRQGLREGA
Sbjct: 361 KTMARMLGNNEAAFEAARSQFWVVDAQGLITEERENLDQDASPFARKVKEINRQGLREGA 420

Query: 421 SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPEEAF 480
           SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPEEAF
Sbjct: 421 SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPEEAF 480

Query: 481 SILGENVIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAA 540
           SILGE+VIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAA
Sbjct: 481 SILGESVIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAA 540

Query: 541 AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYREVDARELRKF 600
           AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYR VDARELRKF
Sbjct: 541 AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYRGVDARELRKF 600

Query: 601 SKEEILEFVKNNMWSPEYPTLVYNQD 627
           SKEEILEFVKNNMWSPEYPTLVYNQD
Sbjct: 601 SKEEILEFVKNNMWSPEYPTLVYNQD 626

[Close]

BLAST of PI0000230 vs. ExPASy TrEMBL
Match: A0A0A0L7T0 (Malic enzyme OS=Cucumis sativus OX=3659 GN=Csa_3G127830 PE=3 SV=1)

HSP 1 Score: 1226.1 bits (3171), Expect = 0.0e+00
Identity = 617/626 (98.56%), Postives = 620/626 (99.04%), Query Frame = 0


Query: 1   MTKWNSLSTSLMKRLKLHYQMTNSIPALAQARSFTTSEGHRPTIVHKRSLDILHDPWFNK 60
           MTKWNSLS SLMKRLKLHYQM NSIPALAQARSFTTSEGHRPTIVHKRSLDILHDPWFNK
Sbjct: 1   MTKWNSLSASLMKRLKLHYQMINSIPALAQARSFTTSEGHRPTIVHKRSLDILHDPWFNK 60

Query: 61  GTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMFDLKRLEVQARDGPSDPNALAKWRILN 120
           GTAFT+TERDRLDLRGLLPPNVMSSEQQIERFM DLKRLEVQARDGPSDPNALAKWRILN
Sbjct: 61  GTAFTITERDRLDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPNALAKWRILN 120

Query: 121 RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSM 180
           RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSA+DRGEMMSM
Sbjct: 121 RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAQDRGEMMSM 180

Query: 181 VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG 240
           VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG
Sbjct: 181 VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG 240

Query: 241 TNNEKLRKDPLYLGLQQQRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ 300
           TNNEKL KDPLYLGLQQ RLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ
Sbjct: 241 TNNEKLLKDPLYLGLQQHRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ 300

Query: 301 RYRNTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR 360
           RYRNTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR
Sbjct: 301 RYRNTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR 360

Query: 361 KTMARMLGNNEAAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGA 420
           KTMARMLGNNEAAFEAARSQFWVVDAQGLITEER+NIDQDASPFARKVKEINRQGLREGA
Sbjct: 361 KTMARMLGNNEAAFEAARSQFWVVDAQGLITEERKNIDQDASPFARKVKEINRQGLREGA 420

Query: 421 SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPEEAF 480
           SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPEEAF
Sbjct: 421 SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPEEAF 480

Query: 481 SILGENVIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAA 540
           SILGENVIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAA
Sbjct: 481 SILGENVIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAA 540

Query: 541 AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYREVDARELRKF 600
           AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYR VDARELRKF
Sbjct: 541 AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYRGVDARELRKF 600

Query: 601 SKEEILEFVKNNMWSPEYPTLVYNQD 627
           SKEEILEFVKNNMWSPEYPTLVYNQD
Sbjct: 601 SKEEILEFVKNNMWSPEYPTLVYNQD 626

[Close]

BLAST of PI0000230 vs. ExPASy TrEMBL
Match: A0A6J1IQW3 (Malic enzyme OS=Cucurbita maxima OX=3661 GN=LOC111479676 PE=3 SV=1)

HSP 1 Score: 1223.0 bits (3163), Expect = 0.0e+00
Identity = 613/626 (97.92%), Postives = 620/626 (99.04%), Query Frame = 0


Query: 1   MTKWNSLSTSLMKRLKLHYQMTNSIPALAQARSFTTSEGHRPTIVHKRSLDILHDPWFNK 60
           MTKWNSLS SLMKRLKLHYQM+NSIPALAQARSFTTSEGHRP I+HKRSLDILHDPWFNK
Sbjct: 1   MTKWNSLSASLMKRLKLHYQMSNSIPALAQARSFTTSEGHRPAIIHKRSLDILHDPWFNK 60

Query: 61  GTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMFDLKRLEVQARDGPSDPNALAKWRILN 120
           GTAFTMTERDRLDLRGLLPPNVMSSEQQIERFM DLKRLEVQARDGPSDPNALAKWRILN
Sbjct: 61  GTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPNALAKWRILN 120

Query: 121 RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSM 180
           RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSM
Sbjct: 121 RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSM 180

Query: 181 VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG 240
           VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG
Sbjct: 181 VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG 240

Query: 241 TNNEKLRKDPLYLGLQQQRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ 300
           TNNEKL KDPLYLGLQQ RLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ
Sbjct: 241 TNNEKLLKDPLYLGLQQNRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ 300

Query: 301 RYRNTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR 360
           RYRNTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR
Sbjct: 301 RYRNTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR 360

Query: 361 KTMARMLGNNEAAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGA 420
           KTMARMLGNNE+AFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGA
Sbjct: 361 KTMARMLGNNESAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGA 420

Query: 421 SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPEEAF 480
           SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPE+AF
Sbjct: 421 SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPEQAF 480

Query: 481 SILGENVIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAA 540
           SILGENVIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSG+PIVSDGMLQAA
Sbjct: 481 SILGENVIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGAPIVSDGMLQAA 540

Query: 541 AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYREVDARELRKF 600
           AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYR VD+RELRKF
Sbjct: 541 AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYRGVDSRELRKF 600

Query: 601 SKEEILEFVKNNMWSPEYPTLVYNQD 627
           SKE+ILEFVKNNMWSPEYPTLVYNQD
Sbjct: 601 SKEQILEFVKNNMWSPEYPTLVYNQD 626

[Close]

BLAST of PI0000230 vs. ExPASy TrEMBL
Match: A0A6J1EA17 (Malic enzyme OS=Cucurbita moschata OX=3662 GN=LOC111432147 PE=3 SV=1)

HSP 1 Score: 1219.9 bits (3155), Expect = 0.0e+00
Identity = 611/626 (97.60%), Postives = 619/626 (98.88%), Query Frame = 0


Query: 1   MTKWNSLSTSLMKRLKLHYQMTNSIPALAQARSFTTSEGHRPTIVHKRSLDILHDPWFNK 60
           MTKWNSLS SLMKRLKLHYQM+NSIPAL+Q RSFTTSEGHRP I+HKRSLDILHDPWFNK
Sbjct: 1   MTKWNSLSASLMKRLKLHYQMSNSIPALSQVRSFTTSEGHRPAIIHKRSLDILHDPWFNK 60

Query: 61  GTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMFDLKRLEVQARDGPSDPNALAKWRILN 120
           GTAFTMTERDRLDLRGLLPPNVMSSEQQIERFM DLKRLEVQARDGPSDPNALAKWRILN
Sbjct: 61  GTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPNALAKWRILN 120

Query: 121 RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSM 180
           RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSM
Sbjct: 121 RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSM 180

Query: 181 VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG 240
           VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG
Sbjct: 181 VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG 240

Query: 241 TNNEKLRKDPLYLGLQQQRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ 300
           TNNEKL KDPLYLGLQQ RLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ
Sbjct: 241 TNNEKLLKDPLYLGLQQNRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ 300

Query: 301 RYRNTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR 360
           RYRNTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR
Sbjct: 301 RYRNTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR 360

Query: 361 KTMARMLGNNEAAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGA 420
           KTMARMLGNNE+AFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGA
Sbjct: 361 KTMARMLGNNESAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGA 420

Query: 421 SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPEEAF 480
           SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPE+AF
Sbjct: 421 SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPEQAF 480

Query: 481 SILGENVIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAA 540
           SILGENVIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSG+PIVSDGMLQAA
Sbjct: 481 SILGENVIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGAPIVSDGMLQAA 540

Query: 541 AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYREVDARELRKF 600
           AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYR VDAREL+KF
Sbjct: 541 AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYRGVDARELQKF 600

Query: 601 SKEEILEFVKNNMWSPEYPTLVYNQD 627
           SKE+ILEFVKNNMWSPEYPTLVYNQD
Sbjct: 601 SKEQILEFVKNNMWSPEYPTLVYNQD 626

[Close]

BLAST of PI0000230 vs. ExPASy TrEMBL
Match: A0A5D3D1H1 (Malic enzyme OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003240 PE=3 SV=1)

HSP 1 Score: 1209.5 bits (3128), Expect = 0.0e+00
Identity = 607/615 (98.70%), Postives = 611/615 (99.35%), Query Frame = 0


Query: 12  MKRLKLHYQMTNSIPALAQARSFTTSEGHRPTIVHKRSLDILHDPWFNKGTAFTMTERDR 71
           MKRLKLHYQMTNSIPALAQ+RSFTTSEGHRPTIVHKRSLDILHDPWFNKGTAFTMTERDR
Sbjct: 1   MKRLKLHYQMTNSIPALAQSRSFTTSEGHRPTIVHKRSLDILHDPWFNKGTAFTMTERDR 60

Query: 72  LDLRGLLPPNVMSSEQQIERFMFDLKRLEVQARDGPSDPNALAKWRILNRLHDRNETMYY 131
           LDLRGLLPPNVMSSEQQIERFM DLKRLEVQARDGPSDPNALAKWRILNRLHDRNETMYY
Sbjct: 61  LDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPNALAKWRILNRLHDRNETMYY 120

Query: 132 KVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSMVYNWPADQVDM 191
           KVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSMVYNWPADQVDM
Sbjct: 121 KVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSMVYNWPADQVDM 180

Query: 192 IVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVGTNNEKLRKDPL 251
           IVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVGTNNEKL KDPL
Sbjct: 181 IVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVGTNNEKLLKDPL 240

Query: 252 YLGLQQQRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQRYRNTYRMFND 311
           YLGLQQ RLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQRYRNTYRMFND
Sbjct: 241 YLGLQQHRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQRYRNTYRMFND 300

Query: 312 DVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAARKTMARMLGNNE 371
           DVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAARKTMARMLGNNE
Sbjct: 301 DVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAARKTMARMLGNNE 360

Query: 372 AAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGASLVEVVQQVKP 431
           AAFEAARSQFWVVDAQGLITEEREN+DQDASPFARKVKEINRQGLREGASLVEVVQQVKP
Sbjct: 361 AAFEAARSQFWVVDAQGLITEERENLDQDASPFARKVKEINRQGLREGASLVEVVQQVKP 420

Query: 432 DVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPEEAFSILGENVIFAS 491
           DVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPEEAFSILGE+VIFAS
Sbjct: 421 DVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPEEAFSILGESVIFAS 480

Query: 492 GSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAAAECLAAYMTED 551
           GSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAAAECLAAYMTED
Sbjct: 481 GSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAAAECLAAYMTED 540

Query: 552 EVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYREVDARELRKFSKEEILEFVKN 611
           EVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYR +DARELRKFSKEEILEFVKN
Sbjct: 541 EVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYRGIDARELRKFSKEEILEFVKN 600

Query: 612 NMWSPEYPTLVYNQD 627
           NMWSPEYPTLVYNQD
Sbjct: 601 NMWSPEYPTLVYNQD 615

[Close]

BLAST of PI0000230 vs. NCBI nr
Match: XP_008438266.1 (PREDICTED: NAD-dependent malic enzyme 62 kDa isoform, mitochondrial isoform X1 [Cucumis melo])

HSP 1 Score: 1228.8 bits (3178), Expect = 0.0e+00
Identity = 618/626 (98.72%), Postives = 621/626 (99.20%), Query Frame = 0


Query: 1   MTKWNSLSTSLMKRLKLHYQMTNSIPALAQARSFTTSEGHRPTIVHKRSLDILHDPWFNK 60
           MTKWNSLS SLMKRLKLHYQMTNSIPALAQ+RSFTTSEGHRPTIVHKRSLDILHDPWFNK
Sbjct: 1   MTKWNSLSASLMKRLKLHYQMTNSIPALAQSRSFTTSEGHRPTIVHKRSLDILHDPWFNK 60

Query: 61  GTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMFDLKRLEVQARDGPSDPNALAKWRILN 120
           GTAFTMTERDRLDLRGLLPPNVMSSEQQIERFM DLKRLEVQARDGPSDPNALAKWRILN
Sbjct: 61  GTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPNALAKWRILN 120

Query: 121 RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSM 180
           RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSM
Sbjct: 121 RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSM 180

Query: 181 VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG 240
           VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG
Sbjct: 181 VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG 240

Query: 241 TNNEKLRKDPLYLGLQQQRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ 300
           TNNEKL KDPLYLGLQQ RLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ
Sbjct: 241 TNNEKLLKDPLYLGLQQHRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ 300

Query: 301 RYRNTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR 360
           RYRNTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR
Sbjct: 301 RYRNTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR 360

Query: 361 KTMARMLGNNEAAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGA 420
           KTMARMLGNNEAAFEAARSQFWVVDAQGLITEEREN+DQDASPFARKVKEINRQGLREGA
Sbjct: 361 KTMARMLGNNEAAFEAARSQFWVVDAQGLITEERENLDQDASPFARKVKEINRQGLREGA 420

Query: 421 SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPEEAF 480
           SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPEEAF
Sbjct: 421 SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPEEAF 480

Query: 481 SILGENVIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAA 540
           SILGE+VIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAA
Sbjct: 481 SILGESVIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAA 540

Query: 541 AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYREVDARELRKF 600
           AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYR VDARELRKF
Sbjct: 541 AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYRGVDARELRKF 600

Query: 601 SKEEILEFVKNNMWSPEYPTLVYNQD 627
           SKEEILEFVKNNMWSPEYPTLVYNQD
Sbjct: 601 SKEEILEFVKNNMWSPEYPTLVYNQD 626

[Close]

BLAST of PI0000230 vs. NCBI nr
Match: XP_004133957.1 (NAD-dependent malic enzyme 1, mitochondrial isoform X1 [Cucumis sativus] >KGN56672.1 hypothetical protein Csa_011430 [Cucumis sativus])

HSP 1 Score: 1226.1 bits (3171), Expect = 0.0e+00
Identity = 617/626 (98.56%), Postives = 620/626 (99.04%), Query Frame = 0


Query: 1   MTKWNSLSTSLMKRLKLHYQMTNSIPALAQARSFTTSEGHRPTIVHKRSLDILHDPWFNK 60
           MTKWNSLS SLMKRLKLHYQM NSIPALAQARSFTTSEGHRPTIVHKRSLDILHDPWFNK
Sbjct: 1   MTKWNSLSASLMKRLKLHYQMINSIPALAQARSFTTSEGHRPTIVHKRSLDILHDPWFNK 60

Query: 61  GTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMFDLKRLEVQARDGPSDPNALAKWRILN 120
           GTAFT+TERDRLDLRGLLPPNVMSSEQQIERFM DLKRLEVQARDGPSDPNALAKWRILN
Sbjct: 61  GTAFTITERDRLDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPNALAKWRILN 120

Query: 121 RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSM 180
           RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSA+DRGEMMSM
Sbjct: 121 RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAQDRGEMMSM 180

Query: 181 VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG 240
           VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG
Sbjct: 181 VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG 240

Query: 241 TNNEKLRKDPLYLGLQQQRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ 300
           TNNEKL KDPLYLGLQQ RLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ
Sbjct: 241 TNNEKLLKDPLYLGLQQHRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ 300

Query: 301 RYRNTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR 360
           RYRNTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR
Sbjct: 301 RYRNTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR 360

Query: 361 KTMARMLGNNEAAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGA 420
           KTMARMLGNNEAAFEAARSQFWVVDAQGLITEER+NIDQDASPFARKVKEINRQGLREGA
Sbjct: 361 KTMARMLGNNEAAFEAARSQFWVVDAQGLITEERKNIDQDASPFARKVKEINRQGLREGA 420

Query: 421 SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPEEAF 480
           SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPEEAF
Sbjct: 421 SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPEEAF 480

Query: 481 SILGENVIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAA 540
           SILGENVIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAA
Sbjct: 481 SILGENVIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAA 540

Query: 541 AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYREVDARELRKF 600
           AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYR VDARELRKF
Sbjct: 541 AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYRGVDARELRKF 600

Query: 601 SKEEILEFVKNNMWSPEYPTLVYNQD 627
           SKEEILEFVKNNMWSPEYPTLVYNQD
Sbjct: 601 SKEEILEFVKNNMWSPEYPTLVYNQD 626

[Close]

BLAST of PI0000230 vs. NCBI nr
Match: XP_023528517.1 (NAD-dependent malic enzyme 1, mitochondrial isoform X1 [Cucurbita pepo subsp. pepo] >KAG6582518.1 NAD-dependent malic enzyme 1, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1224.2 bits (3166), Expect = 0.0e+00
Identity = 614/626 (98.08%), Postives = 620/626 (99.04%), Query Frame = 0


Query: 1   MTKWNSLSTSLMKRLKLHYQMTNSIPALAQARSFTTSEGHRPTIVHKRSLDILHDPWFNK 60
           MTKWNSLS SLMKRLKLHYQM+NSIPALAQARSFTTSEGHRP I+HKRSLDILHDPWFNK
Sbjct: 1   MTKWNSLSASLMKRLKLHYQMSNSIPALAQARSFTTSEGHRPAIIHKRSLDILHDPWFNK 60

Query: 61  GTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMFDLKRLEVQARDGPSDPNALAKWRILN 120
           GTAFTMTERDRLDLRGLLPPNVMSSEQQIERFM DLKRLEVQARDGPSDPNALAKWRILN
Sbjct: 61  GTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPNALAKWRILN 120

Query: 121 RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSM 180
           RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSM
Sbjct: 121 RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSM 180

Query: 181 VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG 240
           VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG
Sbjct: 181 VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG 240

Query: 241 TNNEKLRKDPLYLGLQQQRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ 300
           TNNEKL KDPLYLGLQQ RLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ
Sbjct: 241 TNNEKLLKDPLYLGLQQNRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ 300

Query: 301 RYRNTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR 360
           RYRNTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR
Sbjct: 301 RYRNTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR 360

Query: 361 KTMARMLGNNEAAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGA 420
           KTMARMLGNNE+AFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGA
Sbjct: 361 KTMARMLGNNESAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGA 420

Query: 421 SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPEEAF 480
           SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPE+AF
Sbjct: 421 SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPEQAF 480

Query: 481 SILGENVIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAA 540
           SILGENVIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSG+PIVSDGMLQAA
Sbjct: 481 SILGENVIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGAPIVSDGMLQAA 540

Query: 541 AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYREVDARELRKF 600
           AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYR VDARELRKF
Sbjct: 541 AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYRGVDARELRKF 600

Query: 601 SKEEILEFVKNNMWSPEYPTLVYNQD 627
           SKE+ILEFVKNNMWSPEYPTLVYNQD
Sbjct: 601 SKEQILEFVKNNMWSPEYPTLVYNQD 626

[Close]

BLAST of PI0000230 vs. NCBI nr
Match: XP_022980252.1 (NAD-dependent malic enzyme 1, mitochondrial isoform X1 [Cucurbita maxima])

HSP 1 Score: 1223.0 bits (3163), Expect = 0.0e+00
Identity = 613/626 (97.92%), Postives = 620/626 (99.04%), Query Frame = 0


Query: 1   MTKWNSLSTSLMKRLKLHYQMTNSIPALAQARSFTTSEGHRPTIVHKRSLDILHDPWFNK 60
           MTKWNSLS SLMKRLKLHYQM+NSIPALAQARSFTTSEGHRP I+HKRSLDILHDPWFNK
Sbjct: 1   MTKWNSLSASLMKRLKLHYQMSNSIPALAQARSFTTSEGHRPAIIHKRSLDILHDPWFNK 60

Query: 61  GTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMFDLKRLEVQARDGPSDPNALAKWRILN 120
           GTAFTMTERDRLDLRGLLPPNVMSSEQQIERFM DLKRLEVQARDGPSDPNALAKWRILN
Sbjct: 61  GTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPNALAKWRILN 120

Query: 121 RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSM 180
           RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSM
Sbjct: 121 RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSM 180

Query: 181 VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG 240
           VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG
Sbjct: 181 VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG 240

Query: 241 TNNEKLRKDPLYLGLQQQRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ 300
           TNNEKL KDPLYLGLQQ RLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ
Sbjct: 241 TNNEKLLKDPLYLGLQQNRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ 300

Query: 301 RYRNTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR 360
           RYRNTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR
Sbjct: 301 RYRNTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR 360

Query: 361 KTMARMLGNNEAAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGA 420
           KTMARMLGNNE+AFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGA
Sbjct: 361 KTMARMLGNNESAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGA 420

Query: 421 SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPEEAF 480
           SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPE+AF
Sbjct: 421 SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPEQAF 480

Query: 481 SILGENVIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAA 540
           SILGENVIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSG+PIVSDGMLQAA
Sbjct: 481 SILGENVIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGAPIVSDGMLQAA 540

Query: 541 AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYREVDARELRKF 600
           AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYR VD+RELRKF
Sbjct: 541 AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYRGVDSRELRKF 600

Query: 601 SKEEILEFVKNNMWSPEYPTLVYNQD 627
           SKE+ILEFVKNNMWSPEYPTLVYNQD
Sbjct: 601 SKEQILEFVKNNMWSPEYPTLVYNQD 626

[Close]

BLAST of PI0000230 vs. NCBI nr
Match: KAG7018901.1 (NAD-dependent malic enzyme 1, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1222.6 bits (3162), Expect = 0.0e+00
Identity = 613/626 (97.92%), Postives = 620/626 (99.04%), Query Frame = 0


Query: 1   MTKWNSLSTSLMKRLKLHYQMTNSIPALAQARSFTTSEGHRPTIVHKRSLDILHDPWFNK 60
           MTKWNSLS SLMKRLKLHYQM+NSIPALAQARSFTTSEGHRP I+HKRSLDILHDPWFNK
Sbjct: 1   MTKWNSLSASLMKRLKLHYQMSNSIPALAQARSFTTSEGHRPAIIHKRSLDILHDPWFNK 60

Query: 61  GTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMFDLKRLEVQARDGPSDPNALAKWRILN 120
           GTAFTMTERDRLDLRGLLPPNVMSSEQQIERFM DLKRLEVQARDGPSDPNALAKWRILN
Sbjct: 61  GTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMVDLKRLEVQARDGPSDPNALAKWRILN 120

Query: 121 RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSM 180
           RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSM
Sbjct: 121 RLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSM 180

Query: 181 VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG 240
           VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG
Sbjct: 181 VYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVG 240

Query: 241 TNNEKLRKDPLYLGLQQQRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ 300
           TNNEKL KDPLYLGLQQ RLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ
Sbjct: 241 TNNEKLLKDPLYLGLQQNRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQ 300

Query: 301 RYRNTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR 360
           RYRNTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR
Sbjct: 301 RYRNTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAAR 360

Query: 361 KTMARMLGNNEAAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGA 420
           KTMARMLGNNE+AFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGA
Sbjct: 361 KTMARMLGNNESAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGA 420

Query: 421 SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPEEAF 480
           SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPE+AF
Sbjct: 421 SLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPEQAF 480

Query: 481 SILGENVIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAA 540
           SILGENVIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSG+PIVSDGMLQAA
Sbjct: 481 SILGENVIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGAPIVSDGMLQAA 540

Query: 541 AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYREVDARELRKF 600
           AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYR VDAREL+KF
Sbjct: 541 AECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYRGVDARELQKF 600

Query: 601 SKEEILEFVKNNMWSPEYPTLVYNQD 627
           SKE+ILEFVKNNMWSPEYPTLVYNQD
Sbjct: 601 SKEQILEFVKNNMWSPEYPTLVYNQD 626

[Close]

BLAST of PI0000230 vs. TAIR 10
Match: AT2G13560.1 (NAD-dependent malic enzyme 1 )

HSP 1 Score: 1067.8 bits (2760), Expect = 3.4e-312
Identity = 530/620 (85.48%), Postives = 579/620 (93.39%), Query Frame = 0


Query: 7   LSTSLMKRLKLHYQMTNSIPALAQARSFTTSEGHRPTIVHKRSLDILHDPWFNKGTAFTM 66
           LS+S + R+ LH ++  S    +  RSFTTSEGHRPTIVHK+ LDILHDPWFNKGTAFTM
Sbjct: 9   LSSSSLSRI-LHRRILYS----SAVRSFTTSEGHRPTIVHKQGLDILHDPWFNKGTAFTM 68

Query: 67  TERDRLDLRGLLPPNVMSSEQQIERFMFDLKRLEVQARDGPSDPNALAKWRILNRLHDRN 126
           TER+RLDLRGLLPPNVM SEQQI RFM DLKRLE QARDGPSDPNALAKWRILNRLHDRN
Sbjct: 69  TERNRLDLRGLLPPNVMDSEQQIFRFMTDLKRLEEQARDGPSDPNALAKWRILNRLHDRN 128

Query: 127 ETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSMVYNWPA 186
           ETMYYKVLI +IEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSMVYNWPA
Sbjct: 129 ETMYYKVLINNIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSMVYNWPA 188

Query: 187 DQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVGTNNEKL 246
           +QVDMIVVTDGSRILGLGDLGVHGIGIA+GKLDLYVAAAGINPQRVLPVMIDVGTNNEKL
Sbjct: 189 EQVDMIVVTDGSRILGLGDLGVHGIGIAVGKLDLYVAAAGINPQRVLPVMIDVGTNNEKL 248

Query: 247 RKDPLYLGLQQQRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQRYRNTY 306
           R DP+YLGLQQ+RL+ D+Y+ +IDEFMEAV+TRWPHVIVQFEDFQSKWAFKLLQRYR TY
Sbjct: 249 RNDPMYLGLQQRRLEDDDYIDVIDEFMEAVYTRWPHVIVQFEDFQSKWAFKLLQRYRCTY 308

Query: 307 RMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAARKTMARM 366
           RMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPK KIVVAGAGSAGIGVLNAARKTMARM
Sbjct: 309 RMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKMKIVVAGAGSAGIGVLNAARKTMARM 368

Query: 367 LGNNEAAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGASLVEVV 426
           LGN E AF++A+SQFWVVDAQGLITE RENID +A PFARK KE+ RQGL+EGA+LVEVV
Sbjct: 369 LGNTETAFDSAQSQFWVVDAQGLITEGRENIDPEAQPFARKTKEMERQGLKEGATLVEVV 428

Query: 427 QQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPEEAFSILGEN 486
           ++VKPDVLLGLSAVGGLF+KEVLEA+KGST+TRPAIFAMSNPT NAECTP++AFSILGEN
Sbjct: 429 REVKPDVLLGLSAVGGLFSKEVLEAMKGSTSTRPAIFAMSNPTKNAECTPQDAFSILGEN 488

Query: 487 VIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAAAECLAA 546
           +IFASGSPFK+V+FGNGH+GHCNQGNNMYLFPGIGLGTLLSG+PIVSDGMLQAA+ECLAA
Sbjct: 489 MIFASGSPFKNVEFGNGHVGHCNQGNNMYLFPGIGLGTLLSGAPIVSDGMLQAASECLAA 548

Query: 547 YMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYREVDARELRKFSKEEIL 606
           YM+E+EV EGIIYP IS IRDITK IAAAVI EAIEEDLVEGYRE+DARE++K  +E ++
Sbjct: 549 YMSEEEVLEGIIYPPISRIRDITKRIAAAVIKEAIEEDLVEGYREMDAREIQKLDEEGLM 608

Query: 607 EFVKNNMWSPEYPTLVYNQD 627
           E+V+NNMW+PEYPTLVY  D
Sbjct: 609 EYVENNMWNPEYPTLVYKDD 623

[Close]

BLAST of PI0000230 vs. TAIR 10
Match: AT4G00570.1 (NAD-dependent malic enzyme 2 )

HSP 1 Score: 773.5 bits (1996), Expect = 1.3e-223
Identity = 383/614 (62.38%), Postives = 478/614 (77.85%), Query Frame = 0


Query: 21  MTNSIPALAQARSFTTSEGHR---------PTIVHKRSLDILHDPWFNKGTAFTMTERDR 80
           M  +I  L++A +   + G R         P IVHKR  DILHDPWFNK T F +TERDR
Sbjct: 2   MWKNIAGLSKAAAAARTHGSRRCFSTAIPGPCIVHKRGADILHDPWFNKDTGFPLTERDR 61

Query: 81  LDLRGLLPPNVMSSEQQIERFMFDLKRLEVQARDGPSDPNALAKWRILNRLHDRNETMYY 140
           L +RGLLPP VM+  QQ +RF+   + LE   +  P +  ALAKWR+LNRLHDRNET+YY
Sbjct: 62  LGIRGLLPPRVMTCVQQCDRFIESFRSLENNTKGEPENVVALAKWRMLNRLHDRNETLYY 121

Query: 141 KVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSMVYNWPADQVDM 200
           +VLI +I+++API+YTPTVGLVCQNYSGL+RRPRGMYFSA+D+GEMMSM+YNWPA QVDM
Sbjct: 122 RVLIDNIKDFAPIIYTPTVGLVCQNYSGLYRRPRGMYFSAKDKGEMMSMIYNWPAPQVDM 181

Query: 201 IVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVGTNNEKLRKDPL 260
           IV+TDGSRILGLGDLGV GIGI IGKLD+YVAAAGINPQRVLP+M+DVGTNNEKL ++ L
Sbjct: 182 IVITDGSRILGLGDLGVQGIGIPIGKLDMYVAAAGINPQRVLPIMLDVGTNNEKLLQNDL 241

Query: 261 YLGLQQQRLDGDEYLAIIDEFMEAVFTRWPHVIVQFEDFQSKWAFKLLQRYRNTYRMFND 320
           YLG++Q RL+G+EYL IIDEFMEA FTRWP  +VQFEDFQ+KWAF  L+RYR  + MFND
Sbjct: 242 YLGVRQPRLEGEEYLEIIDEFMEAAFTRWPKAVVQFEDFQAKWAFGTLERYRKKFCMFND 301

Query: 321 DVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAARKTMARMLGNNE 380
           DVQGTAGVA+AGLLG VRAQGRP+ DF  QKIVV GAGSAG+GV   A + +ARM G +E
Sbjct: 302 DVQGTAGVALAGLLGTVRAQGRPISDFVNQKIVVVGAGSAGLGVTKMAVQAVARMAGISE 361

Query: 381 AAFEAARSQFWVVDAQGLITEERENIDQDASPFARKVKEINRQGLREGASLVEVVQQVKP 440
           +    A   F+++D  GL+T ER  +D  A  FA+   EI     REGAS+VEVV++V+P
Sbjct: 362 S---EATKNFYLIDKDGLVTTERTKLDPGAVLFAKNPAEI-----REGASIVEVVKKVRP 421

Query: 441 DVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPTTNAECTPEEAFSILGENVIFAS 500
            VLLGLS VGG+F +EVL+A++ S + +PAIFAMSNPT NAECT  +AF   G N++FAS
Sbjct: 422 HVLLGLSGVGGIFNEEVLKAMRESDSCKPAIFAMSNPTLNAECTAADAFKHAGGNIVFAS 481

Query: 501 GSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGSPIVSDGMLQAAAECLAAYMTED 560
           GSPF++V+  NG +GH NQ NNMYLFPGIGLGTLLSG+ IV+DGMLQAA+ECLA+YMT++
Sbjct: 482 GSPFENVELENGKVGHVNQANNMYLFPGIGLGTLLSGARIVTDGMLQAASECLASYMTDE 541

Query: 561 EVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGYREVDARELRKFSKEEILEFVKN 620
           EV +GI+YPSI++IR IT E+ AAV+  A+ +D+ EG+ +V  ++L   SKE+ + ++  
Sbjct: 542 EVQKGILYPSINNIRHITAEVGAAVLRAAVTDDIAEGHGDVGPKDLSHMSKEDTVNYITR 601

Query: 621 NMWSPEYPTLVYNQ 626
           NMW P Y  LV+ +
Sbjct: 602 NMWFPVYSPLVHEK 607

[Close]

BLAST of PI0000230 vs. TAIR 10
Match: AT2G19900.1 (NADP-malic enzyme 1 )

HSP 1 Score: 436.0 bits (1120), Expect = 5.0e-122
Identity = 244/569 (42.88%), Postives = 352/569 (61.86%), Query Frame = 0


Query: 52  ILHDPWFNKGTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMFDLKRLEVQARDGPSDPN 111
           +L DP +NKG AFT  ERD   LRGLLPP V+  + Q +R + ++++ +           
Sbjct: 45  LLRDPRYNKGLAFTEKERDTHYLRGLLPPVVLDQKLQEKRLLNNIRQYQF---------- 104

Query: 112 ALAKWRILNRLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSA 171
            L K+  L  L +RNE ++YK+LI ++EE  PIVYTPTVG  CQ +  +FRRP+G++ S 
Sbjct: 105 PLQKYMALTELQERNERLFYKLLIDNVEELLPIVYTPTVGEACQKFGSIFRRPQGLFISL 164

Query: 172 EDRGEMMSMVYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQR 231
           +D+G+++ ++ NWP   + +IVVTDG RILGLGDLG  G+GI +GKL LY A  G+ P  
Sbjct: 165 KDKGKILDVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLALYSALGGVRPSA 224

Query: 232 VLPVMIDVGTNNEKLRKDPLYLGLQQQRLDGDEYLAIIDEFMEAVFTRW-PHVIVQFEDF 291
            LPV IDVGTNNEKL  D  Y+GL+Q+R  G EY  +++EFM AV   +   V++QFEDF
Sbjct: 225 CLPVTIDVGTNNEKLLNDEFYIGLRQKRATGQEYSELLNEFMSAVKQNYGEKVLIQFEDF 284

Query: 292 QSKWAFKLLQRYRNTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGS 351
            +  AF+LL +Y +T+ +FNDD+QGTA V +AGL+ A +    P+ +      +  GAG 
Sbjct: 285 ANHNAFELLAKYSDTHLVFNDDIQGTASVVLAGLVSAQKLTNSPLAE---HTFLFLGAGE 344

Query: 352 AGIGVLNAARKTMARMLGNNEAAFEAARSQFWVVDAQGLITEERENIDQD-ASPFARKVK 411
           AG G+       M++ +    A+ E +R + W+VD++GLI   R++  QD   P+A + +
Sbjct: 345 AGTGIAELIALYMSKQM---NASVEESRKKIWLVDSKGLIVNSRKDSLQDFKKPWAHEHE 404

Query: 412 EINRQGLREGASLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPT 471
            +          L+  ++ +KP VL+G S VG  FTKEV+EA+  S   RP I A+SNPT
Sbjct: 405 PVK--------DLLGAIKAIKPTVLIGSSGVGRSFTKEVIEAM-SSINERPLIMALSNPT 464

Query: 472 TNAECTPEEAFSILGENVIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGS 531
           T +ECT EEA++      IFASGSPF  V++  G +    Q NN Y+FPG GLG ++SG+
Sbjct: 465 TQSECTAEEAYTWSKGRAIFASGSPFDPVEY-EGKVFVSTQANNAYIFPGFGLGLVISGA 524

Query: 532 PIVSDGMLQAAAECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGY 591
             V D ML AAAE LA  ++++   +G+IYPS SSIR I+ +IAA V  +A E  L    
Sbjct: 525 IRVHDDMLLAAAEALAGQVSKENYEKGMIYPSFSSIRKISAQIAANVATKAYELGLAG-- 577

Query: 592 REVDARELRKFSKEEILEFVKNNMWSPEY 619
                   R    ++I++  +++M+SP Y
Sbjct: 585 --------RLPRPKDIVKCAESSMYSPTY 577

[Close]

BLAST of PI0000230 vs. TAIR 10
Match: AT5G11670.1 (NADP-malic enzyme 2 )

HSP 1 Score: 422.5 bits (1085), Expect = 5.7e-118
Identity = 236/569 (41.48%), Postives = 340/569 (59.75%), Query Frame = 0


Query: 52  ILHDPWFNKGTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMFDLKRLEVQARDGPSDPN 111
           ++ DP +NKG AFT  ERD   L GLLPP ++S + Q  + M +L++  V          
Sbjct: 52  LMRDPRYNKGLAFTDKERDAHYLTGLLPPVILSQDVQERKVMHNLRQYTV---------- 111

Query: 112 ALAKWRILNRLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSA 171
            L ++  L  L +RNE ++YK+LI ++EE  P+VYTPTVG  CQ Y  +FR+P+G+Y S 
Sbjct: 112 PLQRYMALMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRKPQGLYISL 171

Query: 172 EDRGEMMSMVYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQR 231
            ++G+++ ++ NWP   + +IVVTDG RILGLGDLG  G+GI +GKL LY A  GI P  
Sbjct: 172 NEKGKILEVLKNWPQRGIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSA 231

Query: 232 VLPVMIDVGTNNEKLRKDPLYLGLQQQRLDGDEYLAIIDEFMEAVFTRW-PHVIVQFEDF 291
            LP+ IDVGTNNEKL  D  Y+GL+Q+R  G EY   + EFM AV   +   V+VQFEDF
Sbjct: 232 CLPITIDVGTNNEKLLNDEFYIGLKQRRATGQEYAEFLHEFMCAVKQNYGEKVLVQFEDF 291

Query: 292 QSKWAFKLLQRYRNTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGS 351
            +  AF LL +Y +++ +FNDD+QGTA V +AGL+ A +  G+ + D      +  GAG 
Sbjct: 292 ANHNAFDLLSKYSDSHLVFNDDIQGTASVVLAGLIAAQKVLGKKLAD---HTFLFLGAGE 351

Query: 352 AGIGVLNAARKTMARMLGNNEAAFEAARSQFWVVDAQGLITEER-ENIDQDASPFARKVK 411
           AG G+       +++  G   A     R + W+VD++GLI   R E++     P+A + K
Sbjct: 352 AGTGIAELIALKISKETG---APITETRKKIWLVDSKGLIVSSRKESLQHFKQPWAHEHK 411

Query: 412 EINRQGLREGASLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPT 471
            +          L+  V  +KP VL+G S VG  FTKEV+EA+  +   +P I A+SNPT
Sbjct: 412 PVK--------DLIGAVNAIKPTVLIGTSGVGQTFTKEVVEAM-ATNNEKPLILALSNPT 471

Query: 472 TNAECTPEEAFSILGENVIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGS 531
           + AECT E+A++      IF SGSPF  V + +G      Q NN Y+FPG+GLG ++SG+
Sbjct: 472 SQAECTAEQAYTWTKGRAIFGSGSPFDPVVY-DGKTYLPGQANNCYIFPGLGLGLIMSGA 531

Query: 532 PIVSDGMLQAAAECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGY 591
             V D ML AA+E LAA +TE+    G+IYP  S+IR+I+  IAA V  +  +  L    
Sbjct: 532 IRVRDDMLLAASEALAAQVTEEHYANGLIYPPFSNIREISANIAACVAAKTYDLGLASNL 584

Query: 592 REVDARELRKFSKEEILEFVKNNMWSPEY 619
                        +++++F +++M+SP Y
Sbjct: 592 PRA----------KDLVKFAESSMYSPVY 584

[Close]

BLAST of PI0000230 vs. TAIR 10
Match: AT1G79750.1 (NADP-malic enzyme 4 )

HSP 1 Score: 421.0 bits (1081), Expect = 1.7e-117
Identity = 237/569 (41.65%), Postives = 341/569 (59.93%), Query Frame = 0


Query: 52  ILHDPWFNKGTAFTMTERDRLDLRGLLPPNVMSSEQQIERFMFDLKRLEVQARDGPSDPN 111
           +L DP  NKG AF+  ERD   LRGLLPP V+S + Q+++ M  L++ +V          
Sbjct: 110 LLRDPHHNKGLAFSHRERDAHYLRGLLPPTVISQDLQVKKIMHTLRQYQV---------- 169

Query: 112 ALAKWRILNRLHDRNETMYYKVLIAHIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFSA 171
            L K+  +  L + NE ++YK+LI H+EE  P++YTPTVG  CQ Y  +F RP+G++ S 
Sbjct: 170 PLQKYMAMMDLQETNERLFYKLLIDHVEELLPVIYTPTVGEACQKYGSIFLRPQGLFISL 229

Query: 172 EDRGEMMSMVYNWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQR 231
           +++G++  ++ NWP   + +IVVTDG RILGLGDLG  G+GI +GKL LY A  G+ P  
Sbjct: 230 KEKGKIHEVLRNWPEKNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSA 289

Query: 232 VLPVMIDVGTNNEKLRKDPLYLGLQQQRLDGDEYLAIIDEFMEAVFTRW-PHVIVQFEDF 291
            LPV IDVGTNNEKL  D  Y+GL+Q+R  G+EY  ++ EFM AV   +   V++QFEDF
Sbjct: 290 CLPVTIDVGTNNEKLLNDEFYIGLRQRRATGEEYSELMHEFMTAVKQNYGEKVVIQFEDF 349

Query: 292 QSKWAFKLLQRYRNTYRMFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKQKIVVAGAGS 351
            +  AF LL +Y  T+ +FNDD+QGTA V +AGL+ A+R  G  + D    + +  GAG 
Sbjct: 350 ANHNAFDLLAKYGTTHLVFNDDIQGTASVVLAGLIAALRFVGGSLSD---HRFLFLGAGE 409

Query: 352 AGIGVLNAARKTMARMLGNNEAAFEAARSQFWVVDAQGLITEER-ENIDQDASPFARKVK 411
           AG G+       +++    +    E AR   W+VD++GLI   R E+I     P+A    
Sbjct: 410 AGTGIAELIALEISK---KSHIPLEEARKNIWLVDSKGLIVSSRKESIQHFKKPWAH--- 469

Query: 412 EINRQGLREGASLVEVVQQVKPDVLLGLSAVGGLFTKEVLEALKGSTATRPAIFAMSNPT 471
             + + +RE   LV+ V+ +KP VL+G S VG  FT++V+E +      +P I ++SNPT
Sbjct: 470 --DHEPIRE---LVDAVKAIKPTVLIGTSGVGQTFTQDVVETM-AKLNEKPIILSLSNPT 529

Query: 472 TNAECTPEEAFSILGENVIFASGSPFKDVDFGNGHIGHCNQGNNMYLFPGIGLGTLLSGS 531
           + +ECT EEA++      IFASGSPF  V++  G      Q NN Y+FPG GLG ++SG+
Sbjct: 530 SQSECTAEEAYTWSQGRAIFASGSPFAPVEY-EGKTFVPGQANNAYIFPGFGLGLIMSGT 589

Query: 532 PIVSDGMLQAAAECLAAYMTEDEVHEGIIYPSISSIRDITKEIAAAVIMEAIEEDLVEGY 591
             V D ML AA+E LA  + E+   +G+IYP   +IR I+  IAA V  +A E  L    
Sbjct: 590 IRVHDDMLLAASEALAEELMEEHYEKGMIYPPFRNIRKISARIAAKVAAKAYELGLA--- 642

Query: 592 REVDARELRKFSKEEILEFVKNNMWSPEY 619
                   R    +E+ +  +++M+SP Y
Sbjct: 650 -------TRLPQPKELEQCAESSMYSPSY 642

The following BLAST results are available for this feature:

BLAST of PI0000230 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SIU0	4.8e-311	85.48	NAD-dependent malic enzyme 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=N...	[more]
P37221	1.9e-307	84.52	NAD-dependent malic enzyme 62 kDa isoform, mitochondrial OS=Solanum tuberosum OX...	[more]
P37224	5.5e-283	78.35	NAD-dependent malic enzyme 65 kDa isoform, mitochondrial OS=Amaranthus hypochond...	[more]
P37225	1.3e-228	66.84	NAD-dependent malic enzyme 59 kDa isoform, mitochondrial OS=Solanum tuberosum OX...	[more]
Q8L7K9	1.9e-222	62.38	NAD-dependent malic enzyme 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=N...	[more]

BLAST of PI0000230 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3AVM9	0.0e+00	98.72	Malic enzyme OS=Cucumis melo OX=3656 GN=LOC103483428 PE=3 SV=1	[more]
A0A0A0L7T0	0.0e+00	98.56	Malic enzyme OS=Cucumis sativus OX=3659 GN=Csa_3G127830 PE=3 SV=1	[more]
A0A6J1IQW3	0.0e+00	97.92	Malic enzyme OS=Cucurbita maxima OX=3661 GN=LOC111479676 PE=3 SV=1	[more]
A0A6J1EA17	0.0e+00	97.60	Malic enzyme OS=Cucurbita moschata OX=3662 GN=LOC111432147 PE=3 SV=1	[more]
A0A5D3D1H1	0.0e+00	98.70	Malic enzyme OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003240 ...	[more]

BLAST of PI0000230 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008438266.1	0.0e+00	98.72	PREDICTED: NAD-dependent malic enzyme 62 kDa isoform, mitochondrial isoform X1 [...	[more]
XP_004133957.1	0.0e+00	98.56	NAD-dependent malic enzyme 1, mitochondrial isoform X1 [Cucumis sativus] >KGN566...	[more]
XP_023528517.1	0.0e+00	98.08	NAD-dependent malic enzyme 1, mitochondrial isoform X1 [Cucurbita pepo subsp. pe...	[more]
XP_022980252.1	0.0e+00	97.92	NAD-dependent malic enzyme 1, mitochondrial isoform X1 [Cucurbita maxima]	[more]
KAG7018901.1	0.0e+00	97.92	NAD-dependent malic enzyme 1, mitochondrial [Cucurbita argyrosperma subsp. argyr...	[more]

BLAST of PI0000230 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G13560.1	3.4e-312	85.48	NAD-dependent malic enzyme 1	[more]
AT4G00570.1	1.3e-223	62.38	NAD-dependent malic enzyme 2	[more]
AT2G19900.1	5.0e-122	42.88	NADP-malic enzyme 1	[more]
AT5G11670.1	5.7e-118	41.48	NADP-malic enzyme 2	[more]
AT1G79750.1	1.7e-117	41.65	NADP-malic enzyme 4	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001891	Malic oxidoreductase	PRINTS	PR00072	MALOXRDTASE	coord: 225..247 score: 60.22 coord: 309..325 score: 70.74 coord: 343..359 score: 43.82 coord: 284..302 score: 53.95 coord: 457..473 score: 56.76 coord: 189..218 score: 70.25 coord: 129..153 score: 54.1
IPR001891	Malic oxidoreductase	PIRSF	PIRSF000106	ME	coord: 16..624 e-value: 6.5E-221 score: 732.8
IPR012302	Malic enzyme, NAD-binding	SMART	SM00919	Malic_M_2	coord: 313..581 e-value: 7.6E-87 score: 304.5
IPR012302	Malic enzyme, NAD-binding	PFAM	PF03949	Malic_M	coord: 313..580 e-value: 2.5E-91 score: 305.5
IPR012301	Malic enzyme, N-terminal domain	SMART	SM01274	malic_2	coord: 123..303 e-value: 2.5E-97 score: 339.3
IPR012301	Malic enzyme, N-terminal domain	PFAM	PF00390	malic	coord: 123..303 e-value: 7.1E-77 score: 257.3
IPR037062	Malic enzyme, N-terminal domain superfamily	GENE3D	3.40.50.10380		coord: 47..310 e-value: 1.7E-123 score: 412.7
None	No IPR available	GENE3D	3.40.50.720		coord: 311..626 e-value: 3.6E-100 score: 337.2
None	No IPR available	PIRSR	PIRSR000106-1	PIRSR000106-1	coord: 52..394 e-value: 7.2E-121 score: 402.2
None	No IPR available	PIRSR	PIRSR000106-3	PIRSR000106-3	coord: 51..399 e-value: 4.9E-131 score: 435.7
None	No IPR available	PANTHER	PTHR23406:SF78	MALIC ENZYME	coord: 36..622
None	No IPR available	PANTHER	PTHR23406	MALIC ENZYME-RELATED	coord: 36..622
None	No IPR available	CDD	cd05312	NAD_bind_1_malic_enz	coord: 313..616 e-value: 1.29107E-124 score: 367.644
None	No IPR available	SUPERFAMILY	53223	Aminoacid dehydrogenase-like, N-terminal domain	coord: 19..312
IPR015884	Malic enzyme, conserved site	PROSITE	PS00331	MALIC_ENZYMES	coord: 309..325
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 313..622

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0000230.1	PI0000230.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006108	malate metabolic process
biological_process	GO:0006090	pyruvate metabolic process
molecular_function	GO:0004471	malate dehydrogenase (decarboxylating) (NAD+) activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0051287	NAD binding
molecular_function	GO:0042803	protein homodimerization activity
molecular_function	GO:0004470	malic enzyme activity