PI0000219 (gene) Melon (PI 482460) v1

Overview


Name	PI0000219
Type	gene
Organism	Cucumis metuliferus (Melon (PI 482460) v1)
Description	Ribonuclease T(2)
Location	chr04: 4562360 .. 4563392 (-)
RNA-Seq Expression	PI0000219
Synteny	PI0000219

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTTATCGTTTTGTTCTCTCTTCTGTCCATTCTCAATGGCTCAACCTATTTTAATTATTTATTTTTTAAGTTTGGTGTTGTTCGTTAATGCCGTTCCATTTGACTTCTTTCAGCTGGTTCAACAATGGGGCCCAAATGTATGTAATGTTGCTTCTGTCTCCCCGTGTTATACTTCGCCCAAACCCATGTTCACTATTCATGGCTTGTGGCCAAGTAATTATACTAATGCCCGGCTTGTTTGTAATTCAAAACTGCAATATATCAAGGTTATCATCTTCTTTAATATTTGTTATATGTGTTTTCTTATTCCTCTATCTTTACCATTTTTTAACTAATATAATGTTGCAACATGCTTTAATTTTAGCCTACATCACTTGAAACACAACTGGATACATATTGGCCAGATCTAATACATGAGAAGAACAAGTCTCGCTTTTGGAGTCATGAATGGGACGAGCATGGGAAATGTTCAAGCAACTTGTTTAACGTATTTGATTATTTCCGTATTACTTTGGATCTTCGAAAAAAATATGATCTGTTGGCAATCTTAAACGCGGCCGGGCTCAACCCTCCAATCTCCAAAGGCCTTCTATCCCAAGACATCATAACTGCTATTCAAAATACAACCAACAAAAAGCCCGGCGTCCGCTGCAACAAAATTGGCCGTAAACCTCCCAAGTTCCAATTGTATGAAATTGTACTGTGTATCGACAAAGATGGTGTTACCCTCATTAACTGCCCTCCATTTGTTAGTAGCAGTTGTCCAACTCAAATTGCGTGGCTCCTACCACAAGAGAACTCGATTGGTGTTTCGGATTATCCATGAGTCATATGGGAAAAGTTATGTTATTCGATTATTATGCTTACGAGAACTTTTAAGTTTCCTCTTTATATTAAATCAGTATGTTGTGTGTTCACTGTTGTTGTGATCATTAAGTAGTAGTTTGATAAATTTGAATAATTTGCTTTTGGATGTCCAACAGACTAAAGCCTCTCCAAGATATACCCCATGTTTGTAATATTAAATATTTT

mRNA sequence

ATTTTATCGTTTTGTTCTCTCTTCTGTCCATTCTCAATGGCTCAACCTATTTTAATTATTTATTTTTTAAGTTTGGTGTTGTTCGTTAATGCCGTTCCATTTGACTTCTTTCAGCTGGTTCAACAATGGGGCCCAAATGTATGTAATGTTGCTTCTGTCTCCCCGTGTTATACTTCGCCCAAACCCATGTTCACTATTCATGGCTTGTGGCCAAGTAATTATACTAATGCCCGGCTTGTTTGTAATTCAAAACTGCAATATATCAAGCCTACATCACTTGAAACACAACTGGATACATATTGGCCAGATCTAATACATGAGAAGAACAAGTCTCGCTTTTGGAGTCATGAATGGGACGAGCATGGGAAATGTTCAAGCAACTTGTTTAACGTATTTGATTATTTCCGTATTACTTTGGATCTTCGAAAAAAATATGATCTGTTGGCAATCTTAAACGCGGCCGGGCTCAACCCTCCAATCTCCAAAGGCCTTCTATCCCAAGACATCATAACTGCTATTCAAAATACAACCAACAAAAAGCCCGGCGTCCGCTGCAACAAAATTGGCCGTAAACCTCCCAAGTTCCAATTGTATGAAATTGTACTGTGTATCGACAAAGATGGTGTTACCCTCATTAACTGCCCTCCATTTGTTAGTAGCAGTTGTCCAACTCAAATTGCGTGGCTCCTACCACAAGAGAACTCGATTGGTGTTTCGGATTATCCATGAGTCATATGGGAAAAGTTATGTTATTCGATTATTATGCTTACGAGAACTTTTAAGTTTCCTCTTTATATTAAATCAGTATGTTGTGTGTTCACTGTTGTTGTGATCATTAAGTAGTAGTTTGATAAATTTGAATAATTTGCTTTTGGATGTCCAACAGACTAAAGCCTCTCCAAGATATACCCCATGTTTGTAATATTAAATATTTT

Coding sequence (CDS)

ATGGCTCAACCTATTTTAATTATTTATTTTTTAAGTTTGGTGTTGTTCGTTAATGCCGTTCCATTTGACTTCTTTCAGCTGGTTCAACAATGGGGCCCAAATGTATGTAATGTTGCTTCTGTCTCCCCGTGTTATACTTCGCCCAAACCCATGTTCACTATTCATGGCTTGTGGCCAAGTAATTATACTAATGCCCGGCTTGTTTGTAATTCAAAACTGCAATATATCAAGCCTACATCACTTGAAACACAACTGGATACATATTGGCCAGATCTAATACATGAGAAGAACAAGTCTCGCTTTTGGAGTCATGAATGGGACGAGCATGGGAAATGTTCAAGCAACTTGTTTAACGTATTTGATTATTTCCGTATTACTTTGGATCTTCGAAAAAAATATGATCTGTTGGCAATCTTAAACGCGGCCGGGCTCAACCCTCCAATCTCCAAAGGCCTTCTATCCCAAGACATCATAACTGCTATTCAAAATACAACCAACAAAAAGCCCGGCGTCCGCTGCAACAAAATTGGCCGTAAACCTCCCAAGTTCCAATTGTATGAAATTGTACTGTGTATCGACAAAGATGGTGTTACCCTCATTAACTGCCCTCCATTTGTTAGTAGCAGTTGTCCAACTCAAATTGCGTGGCTCCTACCACAAGAGAACTCGATTGGTGTTTCGGATTATCCATGA

Protein sequence

MAQPILIIYFLSLVLFVNAVPFDFFQLVQQWGPNVCNVASVSPCYTSPKPMFTIHGLWPSNYTNARLVCNSKLQYIKPTSLETQLDTYWPDLIHEKNKSRFWSHEWDEHGKCSSNLFNVFDYFRITLDLRKKYDLLAILNAAGLNPPISKGLLSQDIITAIQNTTNKKPGVRCNKIGRKPPKFQLYEIVLCIDKDGVTLINCPPFVSSSCPTQIAWLLPQENSIGVSDYP

Homology

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BLAST of PI0000219 vs. ExPASy Swiss-Prot
Match: P23540 (Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 5.7e-28
Identity = 69/185 (37.30%), Postives = 99/185 (53.51%), Query Frame = 0


Query: 22  FDFFQLVQQWGPNVCNVASVSPCYTSPKPMFTIHGLWPSNYTNARLVC--NSKLQYIKPT 81
           FD F  VQQW P VC+      C  S    FTIHGLWP     +   C   S     K +
Sbjct: 1   FDSFWFVQQWPPAVCSFQKSGSCPGSGLRTFTIHGLWPQGSGTSLTNCPQGSPFDITKIS 60

Query: 82  SLETQLDTYWPDLIHEKNKSRFWSHEWDEHGKCSSNLFNVFDYFRITLDLRKKYDLLAIL 141
            L++QL+T WP+++   N+ +FWSHEW +HG CS + FN   YF++ +D+R  YD++  L
Sbjct: 61  HLQSQLNTLWPNVLRANNQ-QFWSHEWTKHGTCSESTFNQAAYFKLAVDMRNNYDIIGAL 120

Query: 142 --NAAGLNPPISKGLLSQDIITAIQNTTNKKPGVRCNKIGRKPPKFQLYEIVLCIDKDGV 201
             +AAG   P  +    Q I   ++    K PG+RC    +    + L ++V C  +DG 
Sbjct: 121 RPHAAG---PNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSY-LVQVVACFAQDGS 180

Query: 202 TLINC 203
           TLI+C
Sbjct: 181 TLIDC 180

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BLAST of PI0000219 vs. ExPASy Swiss-Prot
Match: P42813 (Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 4.8e-27
Identity = 71/201 (35.32%), Postives = 101/201 (50.25%), Query Frame = 0


Query: 22  FDFFQLVQQWGPNVCNVASVSPCYTS---PKPMFTIHGLWPSNYTNARLV--CNSKLQYI 81
           FDFF  VQQW  + C+      CY +   P   F IHGLWP NY +      C++   + 
Sbjct: 30  FDFFYFVQQWPGSYCDTQK-KCCYPNSGKPAADFGIHGLWP-NYKDGTYPSNCDASKPFD 89

Query: 82  KPT--SLETQLDTYWPDLIHEKNKSR-FWSHEWDEHGKCSSNLFNVFDYFRITLDLRKKY 141
             T   L T +   WP L         FW HEW++HG CS ++ +  +YF+  L+L++K 
Sbjct: 90  SSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSESVIDQHEYFQTALNLKQKT 149

Query: 142 DLLAILNAAGLNPPISKGLLSQDIITAIQNTTNKKPGVRCNKIGRKPPKFQLYEIVLCID 201
           +LL  L  AG+NP   K    + I  +I+ +    P V CN+ G      QLY++ LC+D
Sbjct: 150 NLLGALTKAGINPD-GKSYSLESIRDSIKESIGFTPWVECNRDG--SGNSQLYQVYLCVD 209

Query: 202 KDGVTLINCPPFVSSSCPTQI 215
           + G  LI CP F    C  +I
Sbjct: 210 RSGSGLIECPVFPHGKCGAEI 225

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BLAST of PI0000219 vs. ExPASy Swiss-Prot
Match: P93460 (Ribonuclease S-5 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 3.8e-24
Identity = 76/217 (35.02%), Postives = 108/217 (49.77%), Query Frame = 0


Query: 6   LIIYFLSLVLFVNAVPFDFFQLVQQWGPNVCNVASVSPCYTSPKPMFTIHGLWPSNYTNA 65
           ++   + L+L  + V +D+FQ  QQ+   VCN ++ +PC   P  +FT+HGLWPS+    
Sbjct: 12  MVFLLIVLILSSSTVGYDYFQFTQQYQLAVCN-SNRTPCKDPPDKLFTVHGLWPSSMAGP 71

Query: 66  RLVCNSKLQYIKPTS--LETQLDTYWPDLIHEKNKSRFWSHEWDEHGKCS-SNLFNVFDY 125
               N  ++ I+     LE QL   WP++        FW  EW +HG C    + N   Y
Sbjct: 72  D-PSNCPIRNIRKREKLLEPQLAIIWPNVFDRTKNKLFWDKEWMKHGTCGYPTIDNENHY 131

Query: 126 FR--ITLDLRKKYDLLAILNAAGLNPPISKGLLSQDIITAIQN-TTNKKPGVRCNKIGRK 185
           F   I + + KK ++  IL+ A + P   K  L  DI  AI+N   NKKP ++C K G  
Sbjct: 132 FETVIKMYISKKQNVSRILSKAKIEPDGKKRAL-LDIENAIRNGADNKKPKLKCQKKGTT 191

Query: 186 PPKFQLYEIVLCIDKDGVTLINCP----PFVSSSCPT 213
               +L EI LC DK G   I+CP    P     CPT
Sbjct: 192 T---ELVEITLCSDKSGEHFIDCPHPFEPISPHYCPT 222

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BLAST of PI0000219 vs. ExPASy Swiss-Prot
Match: O80323 (Ribonuclease S-3 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 5.0e-24
Identity = 76/217 (35.02%), Postives = 108/217 (49.77%), Query Frame = 0


Query: 5   ILIIYFLSLVLFVNAVPFDFFQLVQQWGPNVCNVASVSPCYTSPKPMFTIHGLWPSNYTN 64
           +++   + L+L  + V +D+FQ  QQ+   VCN ++ + C   P  +FT+HGLWPSN   
Sbjct: 6   MMVFLLIVLILCSSTVGYDYFQFTQQYQLAVCN-SNRTLCKDPPDKLFTVHGLWPSNMVG 65

Query: 65  A-RLVCNSKLQYIKPTSLETQLDTYWPDLIHEKNKSRFWSHEWDEHGKCS-SNLFNVFDY 124
                C  K    +   LE QL+  WP++      + FW  EW +HG C    + N   Y
Sbjct: 66  PDPSKCPIKNIRKREKLLEHQLEIIWPNVFDRTKNNLFWDKEWMKHGSCGYPTIDNENHY 125

Query: 125 FR--ITLDLRKKYDLLAILNAAGLNPPISKGLLSQDIITAIQN-TTNKKPGVRCNKIGRK 184
           F   I + + KK ++  IL+ A + P   K  L  DI  AI+N   NKKP ++C K G  
Sbjct: 126 FETVIKMYISKKQNVSRILSKAKIEPDGKKRAL-LDIENAIRNGADNKKPKLKCQKKGTT 185

Query: 185 PPKFQLYEIVLCIDKDGVTLINCP----PFVSSSCPT 213
               +L EI LC DK G   I+CP    P     CPT
Sbjct: 186 T---ELVEITLCSDKSGEHFIDCPHPFEPISPHYCPT 217

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BLAST of PI0000219 vs. ExPASy Swiss-Prot
Match: O80322 (Ribonuclease S-1 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 3.6e-22
Identity = 71/221 (32.13%), Postives = 114/221 (51.58%), Query Frame = 0


Query: 6   LIIYFLSLVLFVNAVPFDFFQLVQQWGPNVCNVASVSPCYTSPKPMFTIHGLWPSNYT-- 65
           ++   + L+L  + V +D+FQ  QQ+ P VCN ++ +PC      +FT+HGLWPSN    
Sbjct: 12  MVFSLIVLILSSSTVGYDYFQFTQQYQPAVCN-SNPTPCNDPTDKLFTVHGLWPSNRNGP 71

Query: 66  NARLVCNSKLQYIKPTSLETQLDTYWPDLIHEKNKSRFWSHEWDEHGKCS-SNLFNVFDY 125
           +      + L   K  ++  QL+  WP++++  +   FW  EW +HG C    + +   Y
Sbjct: 72  DPEKCKTTALNSQKIGNMTAQLEIIWPNVLNRSDHVGFWEKEWIKHGTCGYPTIKDDMHY 131

Query: 126 FR--ITLDLRKKYDLLAILNAAGLNPPISKGLLSQDIITAI-QNTTNKKPGVRCNKIGRK 185
            +  I + + +K ++ AIL+ A + P  +   L  DI  AI + T N KP  +C K  R 
Sbjct: 132 LQTVIRMYITQKQNVSAILSKAAIQPNGTNRPL-VDIENAIRRGTNNTKPKFKCQKNTRT 191

Query: 186 PPKFQLYEIVLCIDKDGVTLINCP--PFVSS--SCPTQIAW 217
               +L E+ LC D+D    INCP  P   S  SCP+ + +
Sbjct: 192 TT--ELVEVTLCSDRDLKKFINCPHGPPQGSRFSCPSSVQY 228

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BLAST of PI0000219 vs. ExPASy TrEMBL
Match: A0A0A0LAN6 (Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G745530 PE=3 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 7.7e-68
Identity = 132/218 (60.55%), Postives = 159/218 (72.94%), Query Frame = 0


Query: 1   MAQPILIIYFLSLVLFVN-AVPFDFFQLVQQWGPNVCNV-ASVSPCYTSPKPMFTIHGLW 60
           M Q ILIIYFLSLV+FVN  VPF+FFQ V+QWGPNVCN  A VSPC+T P+  FTIHGLW
Sbjct: 1   MTQSILIIYFLSLVVFVNGGVPFEFFQFVEQWGPNVCNTGARVSPCHTPPQAKFTIHGLW 60

Query: 61  PSNYTNARLVCNSKLQYIKPTSLETQLDTYWPDLIHEKNKSRFWSHEWDEHGKCSSNLFN 120
           PSN++NA L+C SK  Y K  SLE QL+  WPDL +  N  +FW HEWD HGKCS   F+
Sbjct: 61  PSNHSNAHLLCQSKTIYSKVISLEGQLNISWPDLKY-GNNDKFWGHEWDTHGKCSDPPFS 120

Query: 121 VFDYFRITLDLRKKYDLLAILNAAGLNPPISKGLLSQDIITAIQNTTNKKPGVRCNKIGR 180
           +F YF+I+L+L +K+DLL IL AAGLNP  S+ L  Q+I+  IQ TT K PG+RCNK   
Sbjct: 121 LFQYFQISLNLLRKFDLLTILKAAGLNPQTSQNLAIQNIMAPIQRTTKKYPGIRCNK-NV 180

Query: 181 KPPKFQLYEIVLCIDKDGVTLINCPPFVSSSCPTQIAW 217
           K  K QL EIVLC++KDG TLI+CP FVS++C     W
Sbjct: 181 KTGKSQLNEIVLCLEKDGATLIDCPTFVSNTCAKSFVW 216

[Close]

BLAST of PI0000219 vs. ExPASy TrEMBL
Match: A0A0A0LAB0 (Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G736680 PE=3 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 4.8e-62
Identity = 126/237 (53.16%), Postives = 158/237 (66.67%), Query Frame = 0


Query: 1   MAQPILIIYFLSLVLFVNAVPFDFFQLVQQWGPNVCNVASVSPCYTSPKPMFTIHGLWPS 60
           MAQ I I+  LS +LFVN +PFDFFQLVQQWGPN+C+ A  S CY  P+PMFTIHGLWPS
Sbjct: 1   MAQHIFILCLLSSLLFVNGIPFDFFQLVQQWGPNICSPAG-SKCYAQPQPMFTIHGLWPS 60

Query: 61  NYTNARLVCNSKLQYI---KPTSLETQLDTYWPDLIHEKNKSRFWSHEWDEHGKCSSNLF 120
           N++   ++C    +     K TSLE  L+ YWPD+I  KN S FW HEW +H  C    F
Sbjct: 61  NFSTVPILCPRTKKLFPQGKVTSLEQHLNKYWPDVISGKN-SNFWEHEWRKHADCIDPPF 120

Query: 121 NVFDYFRITL--DLRKKYDLLAILNAAGLNPPISKGLLSQDIITAIQNTTNKKPGVRCNK 180
            +F YF I+L   + K YDLL ILN AGL+    K L+SQ+++  I+N T  +PG+RCN 
Sbjct: 121 TIFWYFDISLRHRMNKTYDLLTILNDAGLSHQTHKDLISQNVLDPIKNATGMEPGIRCN- 180

Query: 181 IGRKPPKFQLYEIVLCIDKDGVTLINCPPFVSSSCPTQ--IAWLLPQENSIGVSDYP 231
           I     K QL EIVLC   DG TLI+CP FVS++C +Q    WLLPQ++S+GV DYP
Sbjct: 181 INGITKKLQLKEIVLCFGNDGTTLIDCPSFVSNTCTSQPKFVWLLPQQSSVGVPDYP 234

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BLAST of PI0000219 vs. ExPASy TrEMBL
Match: A0A5D3DP91 (Ribonuclease T(2) OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G002140 PE=3 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 1.6e-57
Identity = 121/235 (51.49%), Postives = 155/235 (65.96%), Query Frame = 0


Query: 1   MAQPILIIYFLSLVLFVNAVPFDFFQLVQQWGPNVCNVASVSPCYTSPKPMFTIHGLWPS 60
           MAQ   I+  LSL+LFVN  PFDFFQLV+QWGPN CN    + C+  P+P FTIHGLWPS
Sbjct: 1   MAQHTFILCLLSLLLFVNGYPFDFFQLVEQWGPNTCN--DGTQCHAQPQPEFTIHGLWPS 60

Query: 61  NYTNARLVCNSKLQYI--KPTSLETQLDTYWPDLIHEKNKSRFWSHEWDEHGKCSSNLFN 120
           N++NARL C ++  +   + + LE  LD YWPD+I+  N+  FWS+EW +HG C    FN
Sbjct: 61  NFSNARLFCATRKLFDNGQISLLEGDLDRYWPDVINGNNRG-FWSNEWKKHGACIDPPFN 120

Query: 121 VFDYFRITLDLRKKYDLLAILNAA---GLNPPISKGLLSQDIITAIQNTTNKKPGVRCNK 180
           +  YF+++LD  + YDLL ILN         P + GL  +DI+  I N TNK  G+RCNK
Sbjct: 121 ITQYFQLSLDHHRTYDLLKILNGVWHRNRQMP-NNGLAPKDILNPIFNATNKTAGIRCNK 180

Query: 181 IGRKPPKFQLYEIVLCIDKDGVTLINCPPFVSSSC-PTQIAWLLPQENSIGVSDY 230
            G    K QL+EIVLC D +G TLI+CP FVS+SC   +  WLLPQ++S GV DY
Sbjct: 181 NG-ITKKLQLHEIVLCFDNNGTTLIDCPKFVSNSCNQPKFVWLLPQQSSDGVPDY 230

[Close]

BLAST of PI0000219 vs. ExPASy TrEMBL
Match: A0A6J1F837 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111441728 PE=3 SV=1)

HSP 1 Score: 217.6 bits (553), Expect = 5.3e-53
Identity = 114/212 (53.77%), Postives = 138/212 (65.09%), Query Frame = 0


Query: 10  FLSLVLFVNAVPFDFFQLVQQWGPNVCNVASVSPCYTSPKPMFTIHGLWPSNYTNARLVC 69
           F+ L+L VNA  FD+FQ+VQQW P  C+   V  C+  P  MFTIHGLWPSNY+NARLVC
Sbjct: 15  FVVLILCVNA-QFDYFQMVQQWPPAKCSSVKVR-CHVQPPTMFTIHGLWPSNYSNARLVC 74

Query: 70  NSKLQYIKPT---SLETQLDTYWPDLIHEKNKSRFWSHEWDEHGKCSSNLFNVFDYFRIT 129
               Q   PT   +L  QL+TYWPD+I  KN+ +FW HEWD HG CS+  FN   YF+IT
Sbjct: 75  AP--QPFNPTQIAALRPQLNTYWPDVIQGKNQ-QFWQHEWDRHGICSNPPFNQLQYFQIT 134

Query: 130 LDLR--KKYDLLAILNAAGLNPPISKGLLSQDIITAIQNTTNKKPGVRCNKIGRKPPKFQ 189
           L++R    YDLL ILNAAGL P  +     +DI  AIQ  T KKPG+RCN   +   K Q
Sbjct: 135 LNIRTNHSYDLLTILNAAGLGPSDTNIREYKDIEGAIQAATGKKPGLRCNINAQSKEKKQ 194

Query: 190 LYEIVLCIDKDGVTLINCPPFVSSSCPTQIAW 217
           L EI+LC DKDG+TLI+C  F   +CP Q  W
Sbjct: 195 LLEIILCFDKDGITLIDCTEFAGITCPPQFVW 221

[Close]

BLAST of PI0000219 vs. ExPASy TrEMBL
Match: A0A6J1EXD5 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111439251 PE=3 SV=1)

HSP 1 Score: 208.0 bits (528), Expect = 4.2e-50
Identity = 113/224 (50.45%), Postives = 134/224 (59.82%), Query Frame = 0


Query: 1   MAQPILIIYFLSLVLFVNAV--PFDFFQLVQQWGPNVCNVASVSPCYTSPKPMFTIHGLW 60
           MAQ   I+     VL  + V   FD+FQ+VQQW P  C   S   CY +P  MFTIHGLW
Sbjct: 1   MAQAPFIVSIFKFVLLTSCVKAQFDYFQMVQQWPPATC---SGVRCYANPPAMFTIHGLW 60

Query: 61  PSNYTNARLVCNSKLQYIKPTSLET---QLDTYWPDLIHEKNKSRFWSHEWDEHGKCSSN 120
           PSNY+N  LVC    Q   P  + T   QL+TYWPD+I   N  RFW HEWD HG CS  
Sbjct: 61  PSNYSNVPLVCAG--QPFNPAQIATLVPQLNTYWPDVI-SGNNQRFWKHEWDSHGTCSDP 120

Query: 121 LFNVFDYFRITLDLR--KKYDLLAILNAAGLNPPISKGLLSQDIITAIQNTTNKKPGVRC 180
            FN   YF+ TL++R    YDLLAILN AGL P  +       I  AIQ  T KKPG+RC
Sbjct: 121 PFNQLQYFQTTLNIRTNHNYDLLAILNTAGLGPTATNTRQYGAIEGAIQAATGKKPGLRC 180

Query: 181 NKIGRKPPKFQLYEIVLCIDKDGVTLINCPPFVSSSCPTQIAWL 218
           NK  +   K QL+EI+LC DK+GVTLI+C  F   +CP+Q  WL
Sbjct: 181 NKNAQSKKK-QLFEIILCFDKNGVTLIDCTQFAGITCPSQFVWL 217

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BLAST of PI0000219 vs. NCBI nr
Match: KAE8651058.1 (hypothetical protein Csa_002465 [Cucumis sativus])

HSP 1 Score: 266.9 bits (681), Expect = 1.6e-67
Identity = 132/218 (60.55%), Postives = 159/218 (72.94%), Query Frame = 0


Query: 1   MAQPILIIYFLSLVLFVN-AVPFDFFQLVQQWGPNVCNV-ASVSPCYTSPKPMFTIHGLW 60
           M Q ILIIYFLSLV+FVN  VPF+FFQ V+QWGPNVCN  A VSPC+T P+  FTIHGLW
Sbjct: 1   MTQSILIIYFLSLVVFVNGGVPFEFFQFVEQWGPNVCNTGARVSPCHTPPQAKFTIHGLW 60

Query: 61  PSNYTNARLVCNSKLQYIKPTSLETQLDTYWPDLIHEKNKSRFWSHEWDEHGKCSSNLFN 120
           PSN++NA L+C SK  Y K  SLE QL+  WPDL +  N  +FW HEWD HGKCS   F+
Sbjct: 61  PSNHSNAHLLCQSKTIYSKVISLEGQLNISWPDLKY-GNNDKFWGHEWDTHGKCSDPPFS 120

Query: 121 VFDYFRITLDLRKKYDLLAILNAAGLNPPISKGLLSQDIITAIQNTTNKKPGVRCNKIGR 180
           +F YF+I+L+L +K+DLL IL AAGLNP  S+ L  Q+I+  IQ TT K PG+RCNK   
Sbjct: 121 LFQYFQISLNLLRKFDLLTILKAAGLNPQTSQNLAIQNIMAPIQRTTKKYPGIRCNK-NV 180

Query: 181 KPPKFQLYEIVLCIDKDGVTLINCPPFVSSSCPTQIAW 217
           K  K QL EIVLC++KDG TLI+CP FVS++C     W
Sbjct: 181 KTGKSQLNEIVLCLEKDGATLIDCPTFVSNTCAKSFVW 216

[Close]

BLAST of PI0000219 vs. NCBI nr
Match: XP_031738690.1 (ribonuclease MC-like [Cucumis sativus])

HSP 1 Score: 266.9 bits (681), Expect = 1.6e-67
Identity = 132/218 (60.55%), Postives = 159/218 (72.94%), Query Frame = 0


Query: 1   MAQPILIIYFLSLVLFVN-AVPFDFFQLVQQWGPNVCNV-ASVSPCYTSPKPMFTIHGLW 60
           M Q ILIIYFLSLV+FVN  VPF+FFQ V+QWGPNVCN  A VSPC+T P+  FTIHGLW
Sbjct: 1   MTQSILIIYFLSLVVFVNGGVPFEFFQFVEQWGPNVCNTGARVSPCHTPPQAKFTIHGLW 60

Query: 61  PSNYTNARLVCNSKLQYIKPTSLETQLDTYWPDLIHEKNKSRFWSHEWDEHGKCSSNLFN 120
           PSN++NA L+C SK  Y K  SLE QL+  WPDL +  N  +FW HEWD HGKCS   F+
Sbjct: 61  PSNHSNAHLLCQSKTIYSKVISLEGQLNISWPDLKY-GNNDKFWGHEWDTHGKCSDPPFS 120

Query: 121 VFDYFRITLDLRKKYDLLAILNAAGLNPPISKGLLSQDIITAIQNTTNKKPGVRCNKIGR 180
           +F YF+I+L+L +K+DLL IL AAGLNP  S+ L  Q+I+  IQ TT K PG+RCNK   
Sbjct: 121 LFQYFQISLNLLRKFDLLTILKAAGLNPQTSQNLAIQNIMAPIQRTTKKYPGIRCNK-NV 180

Query: 181 KPPKFQLYEIVLCIDKDGVTLINCPPFVSSSCPTQIAW 217
           K  K QL EIVLC++KDG TLI+CP FVS++C     W
Sbjct: 181 KTGKSQLNEIVLCLEKDGATLIDCPTFVSNTCAKSFVW 216

[Close]

BLAST of PI0000219 vs. NCBI nr
Match: XP_031738547.1 (ribonuclease MC-like [Cucumis sativus] >XP_031739193.1 ribonuclease MC-like [Cucumis sativus] >KAE8651059.1 hypothetical protein Csa_001394 [Cucumis sativus] >KAE8651065.1 hypothetical protein Csa_001488 [Cucumis sativus])

HSP 1 Score: 257.7 bits (657), Expect = 9.6e-65
Identity = 129/218 (59.17%), Postives = 160/218 (73.39%), Query Frame = 0


Query: 1   MAQPILIIYFLSLVLFVN-AVPFDFFQLVQQWGPNVCNV-ASVSPCYTSPKPMFTIHGLW 60
           M Q ILIIYFLSLV+FVN  VPF+FFQ V+QWGPNVCN  A VSPC+T P+  FTIHGLW
Sbjct: 1   MTQSILIIYFLSLVVFVNGGVPFEFFQFVEQWGPNVCNTGARVSPCHTPPQAKFTIHGLW 60

Query: 61  PSNYTNARLVCNSKLQYIKPTSLETQLDTYWPDLIHEKNKSRFWSHEWDEHGKCSSNLFN 120
           PSN++NA L+C S  +Y K   LE QL+  WPDL    N + FW+HEW+ HGKCS   F+
Sbjct: 61  PSNHSNAHLLCQSITKYTKVLLLERQLNISWPDLKGGMN-NEFWTHEWNFHGKCSDPPFS 120

Query: 121 VFDYFRITLDLRKKYDLLAILNAAGLNPPISKGLLSQDIITAIQNTTNKKPGVRCNKIGR 180
           +F YF+++L+L +K+D+LAIL AAGLNP  S+ +  Q+I+T IQ TT K PG+RC K   
Sbjct: 121 LFQYFQMSLNLLQKFDVLAILKAAGLNPQTSQNIGIQNIMTPIQRTTKKYPGIRCYK-NV 180

Query: 181 KPPKFQLYEIVLCIDKDGVTLINCPPFVSSSCPTQIAW 217
           K  K QL EIVLC++KDG TLI+CP FVSS+C   I W
Sbjct: 181 KTGKSQLNEIVLCLEKDGATLIDCPTFVSSTCAKSIVW 216

[Close]

BLAST of PI0000219 vs. NCBI nr
Match: XP_011651927.1 (ribonuclease MC [Cucumis sativus] >KGN58920.1 hypothetical protein Csa_001884 [Cucumis sativus])

HSP 1 Score: 247.7 bits (631), Expect = 9.9e-62
Identity = 126/237 (53.16%), Postives = 158/237 (66.67%), Query Frame = 0


Query: 1   MAQPILIIYFLSLVLFVNAVPFDFFQLVQQWGPNVCNVASVSPCYTSPKPMFTIHGLWPS 60
           MAQ I I+  LS +LFVN +PFDFFQLVQQWGPN+C+ A  S CY  P+PMFTIHGLWPS
Sbjct: 1   MAQHIFILCLLSSLLFVNGIPFDFFQLVQQWGPNICSPAG-SKCYAQPQPMFTIHGLWPS 60

Query: 61  NYTNARLVCNSKLQYI---KPTSLETQLDTYWPDLIHEKNKSRFWSHEWDEHGKCSSNLF 120
           N++   ++C    +     K TSLE  L+ YWPD+I  KN S FW HEW +H  C    F
Sbjct: 61  NFSTVPILCPRTKKLFPQGKVTSLEQHLNKYWPDVISGKN-SNFWEHEWRKHADCIDPPF 120

Query: 121 NVFDYFRITL--DLRKKYDLLAILNAAGLNPPISKGLLSQDIITAIQNTTNKKPGVRCNK 180
            +F YF I+L   + K YDLL ILN AGL+    K L+SQ+++  I+N T  +PG+RCN 
Sbjct: 121 TIFWYFDISLRHRMNKTYDLLTILNDAGLSHQTHKDLISQNVLDPIKNATGMEPGIRCN- 180

Query: 181 IGRKPPKFQLYEIVLCIDKDGVTLINCPPFVSSSCPTQ--IAWLLPQENSIGVSDYP 231
           I     K QL EIVLC   DG TLI+CP FVS++C +Q    WLLPQ++S+GV DYP
Sbjct: 181 INGITKKLQLKEIVLCFGNDGTTLIDCPSFVSNTCTSQPKFVWLLPQQSSVGVPDYP 234

[Close]

BLAST of PI0000219 vs. NCBI nr
Match: TYK25090.1 (S-RNase [Cucumis melo var. makuwa])

HSP 1 Score: 232.6 bits (592), Expect = 3.3e-57
Identity = 121/235 (51.49%), Postives = 155/235 (65.96%), Query Frame = 0


Query: 1   MAQPILIIYFLSLVLFVNAVPFDFFQLVQQWGPNVCNVASVSPCYTSPKPMFTIHGLWPS 60
           MAQ   I+  LSL+LFVN  PFDFFQLV+QWGPN CN    + C+  P+P FTIHGLWPS
Sbjct: 1   MAQHTFILCLLSLLLFVNGYPFDFFQLVEQWGPNTCN--DGTQCHAQPQPEFTIHGLWPS 60

Query: 61  NYTNARLVCNSKLQYI--KPTSLETQLDTYWPDLIHEKNKSRFWSHEWDEHGKCSSNLFN 120
           N++NARL C ++  +   + + LE  LD YWPD+I+  N+  FWS+EW +HG C    FN
Sbjct: 61  NFSNARLFCATRKLFDNGQISLLEGDLDRYWPDVINGNNRG-FWSNEWKKHGACIDPPFN 120

Query: 121 VFDYFRITLDLRKKYDLLAILNAA---GLNPPISKGLLSQDIITAIQNTTNKKPGVRCNK 180
           +  YF+++LD  + YDLL ILN         P + GL  +DI+  I N TNK  G+RCNK
Sbjct: 121 ITQYFQLSLDHHRTYDLLKILNGVWHRNRQMP-NNGLAPKDILNPIFNATNKTAGIRCNK 180

Query: 181 IGRKPPKFQLYEIVLCIDKDGVTLINCPPFVSSSC-PTQIAWLLPQENSIGVSDY 230
            G    K QL+EIVLC D +G TLI+CP FVS+SC   +  WLLPQ++S GV DY
Sbjct: 181 NG-ITKKLQLHEIVLCFDNNGTTLIDCPKFVSNSCNQPKFVWLLPQQSSDGVPDY 230

[Close]

BLAST of PI0000219 vs. TAIR 10
Match: AT1G14220.1 (Ribonuclease T2 family protein )

HSP 1 Score: 126.7 bits (317), Expect = 2.4e-29
Identity = 75/201 (37.31%), Postives = 103/201 (51.24%), Query Frame = 0


Query: 22  FDFFQLVQQWGPNVCNVASVSPCYTSPKPM--FTIHGLWPSNYTNARLVCN----SKLQY 81
           FDFF  V QW    C+ +      TS KP   F IHGLWP NY       N    S+   
Sbjct: 28  FDFFYFVLQWPGAYCDTSRACCYPTSGKPAADFGIHGLWP-NYNGGSWPSNCDPDSQFDR 87

Query: 82  IKPTSLETQLDTYWPDLIHEKNKS-RFWSHEWDEHGKCSSNLFNVFDYFRITLDLRKKYD 141
            + + L + L   WP L    N+   FW HEW++HG CS ++ +  +YF   L L++K +
Sbjct: 88  SQISDLVSSLKKNWPTLSCPSNEGFNFWEHEWEKHGTCSESVMDQHEYFENALKLKQKAN 147

Query: 142 LLAILNAAGLNPPISKGLLSQDIIT-AIQNTTNKKPGVRCNKIGRKPPKFQLYEIVLCID 201
           LL IL  +G+NP    G  + D IT AI++     PG+ CNK   +    QL++I +C+D
Sbjct: 148 LLQILKNSGINP--DDGFYNLDKITNAIKDGIGFTPGIECNKDPER--NAQLHQIYICVD 207

Query: 202 KDGVTLINCPPFVSSSCPTQI 215
             G   I CP     SCP+QI
Sbjct: 208 TSGTEFIECPVLPRGSCPSQI 223

[Close]

BLAST of PI0000219 vs. TAIR 10
Match: AT2G02990.1 (ribonuclease 1 )

HSP 1 Score: 122.9 bits (307), Expect = 3.4e-28
Identity = 71/201 (35.32%), Postives = 101/201 (50.25%), Query Frame = 0


Query: 22  FDFFQLVQQWGPNVCNVASVSPCYTS---PKPMFTIHGLWPSNYTNARLV--CNSKLQYI 81
           FDFF  VQQW  + C+      CY +   P   F IHGLWP NY +      C++   + 
Sbjct: 30  FDFFYFVQQWPGSYCDTQK-KCCYPNSGKPAADFGIHGLWP-NYKDGTYPSNCDASKPFD 89

Query: 82  KPT--SLETQLDTYWPDLIHEKNKSR-FWSHEWDEHGKCSSNLFNVFDYFRITLDLRKKY 141
             T   L T +   WP L         FW HEW++HG CS ++ +  +YF+  L+L++K 
Sbjct: 90  SSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSESVIDQHEYFQTALNLKQKT 149

Query: 142 DLLAILNAAGLNPPISKGLLSQDIITAIQNTTNKKPGVRCNKIGRKPPKFQLYEIVLCID 201
           +LL  L  AG+NP   K    + I  +I+ +    P V CN+ G      QLY++ LC+D
Sbjct: 150 NLLGALTKAGINPD-GKSYSLESIRDSIKESIGFTPWVECNRDG--SGNSQLYQVYLCVD 209

Query: 202 KDGVTLINCPPFVSSSCPTQI 215
           + G  LI CP F    C  +I
Sbjct: 210 RSGSGLIECPVFPHGKCGAEI 225

[Close]

BLAST of PI0000219 vs. TAIR 10
Match: AT2G39780.1 (ribonuclease 2 )

HSP 1 Score: 87.4 bits (215), Expect = 1.6e-17
Identity = 62/189 (32.80%), Postives = 88/189 (46.56%), Query Frame = 0


Query: 22  FDFFQLVQQWGPNVCN-----VASVSPCYTSPKP-MFTIHGLWPSNYTNARLVC--NSKL 81
           FD+F L  QW    C       +  + C  S  P  FTIHGLWP     +   C   S  
Sbjct: 32  FDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAPTQFTIHGLWPDYNDGSWPSCCYRSDF 91

Query: 82  QYIKPTSLETQLDTYWPDL------IHEKNKSRFWSHEWDEHGKCSSNLF-NVFDYFRIT 141
           +  + ++L   L+ YWP L           K  FW HEW++HG CSS +F + ++YF  T
Sbjct: 92  KEKEISTLMDGLEKYWPSLSCGSPSSCNGGKGSFWGHEWEKHGTCSSPVFHDEYNYFLTT 151

Query: 142 LDLRKKYDLLAILNAAGLNPPISKGLLSQDIITAIQNTTNKKPGVRCNKIGRKPPKFQLY 196
           L+L  K+++  +L  AG     S+      I+TAIQN  +  P V C +         + 
Sbjct: 152 LNLYLKHNVTDVLYQAGYVASNSEKYPLGGIVTAIQNAFHITPEVVCKRDA-------ID 211

The following BLAST results are available for this feature:

BLAST of PI0000219 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P23540	5.7e-28	37.30	Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
P42813	4.8e-27	35.32	Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1	[more]
P93460	3.8e-24	35.02	Ribonuclease S-5 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1	[more]
O80323	5.0e-24	35.02	Ribonuclease S-3 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1	[more]
O80322	3.6e-22	32.13	Ribonuclease S-1 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1	[more]

BLAST of PI0000219 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LAN6	7.7e-68	60.55	Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G745530 PE=3 SV=1	[more]
A0A0A0LAB0	4.8e-62	53.16	Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G736680 PE=3 SV=1	[more]
A0A5D3DP91	1.6e-57	51.49	Ribonuclease T(2) OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G00...	[more]
A0A6J1F837	5.3e-53	53.77	Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111441728 PE=3 SV=1	[more]
A0A6J1EXD5	4.2e-50	50.45	Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111439251 PE=3 SV=1	[more]

BLAST of PI0000219 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8651058.1	1.6e-67	60.55	hypothetical protein Csa_002465 [Cucumis sativus]	[more]
XP_031738690.1	1.6e-67	60.55	ribonuclease MC-like [Cucumis sativus]	[more]
XP_031738547.1	9.6e-65	59.17	ribonuclease MC-like [Cucumis sativus] >XP_031739193.1 ribonuclease MC-like [Cuc...	[more]
XP_011651927.1	9.9e-62	53.16	ribonuclease MC [Cucumis sativus] >KGN58920.1 hypothetical protein Csa_001884 [C...	[more]
TYK25090.1	3.3e-57	51.49	S-RNase [Cucumis melo var. makuwa]	[more]

BLAST of PI0000219 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G14220.1	2.4e-29	37.31	Ribonuclease T2 family protein	[more]
AT2G02990.1	3.4e-28	35.32	ribonuclease 1	[more]
AT2G39780.1	1.6e-17	32.80	ribonuclease 2	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001568	Ribonuclease T2-like	PFAM	PF00445	Ribonuclease_T2	coord: 22..202 e-value: 2.3E-41 score: 142.0
IPR001568	Ribonuclease T2-like	PANTHER	PTHR11240	RIBONUCLEASE T2	coord: 14..213
IPR036430	Ribonuclease T2-like superfamily	GENE3D	3.90.730.10		coord: 17..226 e-value: 1.8E-53 score: 183.7
IPR036430	Ribonuclease T2-like superfamily	SUPERFAMILY	55895	Ribonuclease Rh-like	coord: 22..219
None	No IPR available	PANTHER	PTHR11240:SF49	RIBONUCLEASE 3	coord: 14..213
IPR018188	Ribonuclease T2, His active site 1	PROSITE	PS00530	RNASE_T2_1	coord: 52..59
IPR033697	Ribonuclease T2, eukaryotic	CDD	cd01061	RNase_T2_euk	coord: 22..211 e-value: 9.38147E-65 score: 197.555

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
PI0000219.1	PI0000219.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016070	RNA metabolic process
cellular_component	GO:0110165	cellular anatomical entity
molecular_function	GO:0033897	ribonuclease T2 activity
molecular_function	GO:0003723	RNA binding