PI0000209 (gene) Melon (PI 482460) v1

Overview
NamePI0000209
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
DescriptionNAD(P)-binding Rossmann-fold superfamily protein
Locationchr07: 22377046 .. 22382139 (+)
RNA-Seq ExpressionPI0000209
SyntenyPI0000209
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGATTCTTGTTACTGGGGCATCTGGGTATCTAGGCGGAAGGCTTTGCCGTGCATTATTGAATCGAGGCTTCTCCGTTAGGGCTTTGGTCCGTCCGACCAGCGACCTTTCTTCTCTTCCACATGATCCTTCTGCTCTTGAGCTCGTTCATGGCGATATCACCGATTACCAGTCGCTTCTAGAGGCATGCTCGGGCTGCCATGTCGTTTTCCACGCGGCCGCAATGGTCGAGCCGTGGCTTCCTGATCCATCGAAATTCATTTCTGTAAGTCCTCAGGTTTCTTGAAAAAGTTGATTGAAGTGAAAGTTTCCCGGAAAATGTTTCAGAGATCTGGGTTTCGGCTGAGCTCATCAGATTTCTTTCAAAATTTGACGATATAGTAAATTATCGGGTTGTTTATATTAGGTCAATGTACGAGGGTTGCAGAACGTGTTGCAAGCGGTAAGAGAGACGAAGACAATCGAGAAAATTATTTATACGTCGTCCTTTTTGCGCTTGGCTCAACCGATGGATATGTTGCCGATGAAAAACAGGTGCCATTAGGATTTAAGTTCTCAGAAAATTGTTCTTCCTCCATGAACAAATGCTGTTGACCTTCATTGATGTTTTCAAATTTCTCTGAACGCGACTGTGATAATAGCAGGTTCATCACGAGAAGTTCTTTTGTACAGAGTACGAGAAATCGAAGGCCATTGCTGACAAGATTGCATTGCAAGCAGCATCTGAGGGGATTCCGATAGTGCCGGTTTATCCCGGAGTCATCTATGGTGTTGGAAAAGTCACGGCTGGGAATGTTGTTGCTCGCATGGTAATAACTGCTTTTTCTCTGATAAATTTATGTTGGATGCTTGTTCAAGTCATTGAGTACTAGCTTATCCATCAAAAGATCATTCAGTGGAATAACACTCAGTTTGAATTCATAACTTCATGGTGACAAAATAATCATGGACAAGAAAAATTATTGTTGTTTACACTGGAAATCCCAAGTTAAATCACTTTTGACTGCTCAAATGAGTTTTTAGACTGGCTACTTCCTACTTTTTGTTCTGGTTATTCTTTAGAGTGAGGTTGACGTGACGCTGTGACATTTTTGTAGTGGTGAAATAGAACAATAGTTGAGTGCATTCCAAGATACATTCATATTCACGTATTTCATTTTGTCTTGTGGCAAATTATAATTGAACAATTTTGTGGGATGCACAAAGATAGGTGTTGTCGGATATGCAATCGAAAGTGAATATTCTTTTAGACCACATTTGAAACTTTAGCTAGTTTTCTTATACAGTCTAACGTGAATTTTTAAAACACATAAAAATACATTAAAAAGGTATTTGGTGTTTTCCTGGTAGATAAAATATAATACTCTGGAAAGAAAGACATCTCTACTTTTTGTAAAAGATTGCAAGTTTTAAACCCACTTGCTTGTGATGTAATATTCTCAACACTGTTATAAAAGGTTATCAGGAGCCCACTCTTCATTTTTTTTAATGATGTTCCTAAAAGCTTTTTATAACCTTAGAGGAATTTGTGAAGAAACCTGGTGAATAAATAATTAATGAGGCTGTTTAATGCATTAGCGAGTATACTAACATTAGCCTTTCTCCTCTTCCTTGAGTTCATTACGTTAGGTAATGAAAAATTAGTTAGGTAAAATACATCCTTTTTTAAAAAAATAAAAAAATAAAAATTAAAAAACAAAAAACTAGTGATTGGGTTAGCTTGCATGTATTTCAATTAATCACAACGGAGAACCCTTGACTTTACGACGTTTGAGTTATAAGAAAACTTGTAGAGCATTAAATCCTAAGTAGGTAACTACCTTAGATTGAACTCATTCCCCAAGTCATTTTATTACACCCATGACCCTTATTAACCTCTGTGCCTAACTGTGTTACTTCTTTGGCTCTTTTTTCTCCCTCGCTTTGACCAGATTATTATTCCCCTTCCCATTCATTCGTATCTGTTATTCTACTATCCTATTAACTCATTTGAGAACTGCAGAAGTTATGCAAATTAGATTTTGGTATAAATTATTTTTATTCTTCAATAGAAAAGCATATGCTTATCCATCTTTTGGAAGCCTTGTGAAACTGAAAATGCGTAAATTTGGAATTGCCTGACTTGTTTGTATGAAATCAATACATTTTAATCTGGAAGTATTTTACTGATCTTCATTTGTCACTAGTATGACCTGACTTTGCTTTCTTTAACAATGGGCCATTTGTGTTACTGTCGTTATTTCTAACCAGCTGATTGAACGGTTCAATGGGCGATTACCCGGATATTTAGGCCAAGGAAACGACAAGTTTTCCTTTAGCCACATCGATGACGTTGTAGAAGGGCATATTGCAGTGATGCAGAAAGGTCGAGTAGGTGAGAGGTATCTTCTAACAGGAGAAAATGCATCATTTGTAGAAGTTTTTGATACGGCTGCAGTCATTACTGGTACGAAGAAACCAATCTTTAATATTCCATTGTGGTTAATTGAAACCTATGGCTGGGTTTCAGTTTTCATCTCTCGAATCACAGGGAAACTTCCTCTCATCAGCCCCCCGGTACGTCGTCGAACTCTCCTGGTCTTTATTTTTTGTAATGAAAATTTCTTGCATCTCTTGTACTCAACATGAACAACAGTCTAATAGTTCAGGTGCCATTTGGTCTTTTGGTTTTTAGTTTTTGTAAATTAAGTCTATAAACATTCATTTTATCCTAAAATTTTTGCTTCGTAATCTACTTTTTACCTATGTTTTTAAAAAGCAAATTAAAATTTAAAAACTAGATAAAGTAGTTTCTTTGGGGTGTTTGGTCCACTGAGTGAAGTGTCTTTTTTATACCCATGAATGTCTAGGCCAGCTTTCGCACACCTCGCCTAATCTCACGGTACAATTCACCTGACCTTACATCATTTGAGTGTTAAGGAAACTTGTAAGAAATTAATTCCTAGGTAGGTAGGCATCATAGATTGACTTGACCTCTTAGTCATTTATTGAGACTGTCTCCTTTTTTACCACTAGGCCAACCCTTGATGGTTAGTCCACTTAGTGAAGTTAACAAGTCTAGGGTTAATATGTTAGAATTAAGAAGTTTATATTTGGGGTGTATAGTTATAAGATCGAGTTCTTCGATAATTGTGAAAAGTAGATGAAGGAAATAAGATGGAGTTGCTCGATAAACGTGAAAAATATTTAAAACTTGGAAAGATAGAGTTACTCGATAAATGTCCTATTTCAATGGTGTTCTATTGAATGTGGGCCAAATTGCCTCTAAAATTTTGTTTGTCTTTGAAATTTGGCTTAGATCTCAACTTCTGTACTTGAAAAAGATGCAAAACATCGAAAGAAAAATAAGAGAAAATAGGTTTAATTTTCAAAAACCGAAAACGAAATGCTTACCAAATAGCGCATTAGGATTTTTGCACTGACCATAGAATCCTATTCAATGGGCGAAACACTTTAGCTATTACTATATTAATAAACAACATAATTTGGAGTTGAAGGAATATACCTTTTGTTTTTTTTTCTGTTGTTGGAGCAGACTGTGAAGGTTTTGAGACACCAGTGGGCATATTCGTGTGAGAAAGCAAAACAAGAGCTGGATTATAACCCTAGAAGCTTAAAAGAAGGAATAGAAGAAATGCTAGCATGGCTAAAGAGCTTGGGTTTGATAAAATATTAAGAAAGATGGATCTATGTTGCAACTTTATTTTGTTGCTATAATCTTCCATTTTCTCTGTAATATCACACCGTTCAAAAATAAAGAGCTGCAATACCAAACTTTGGTTATAAACTGCAAAGTTAGCTATAAATCCCCCCTTTTGTACTCTGATAATTTCTTTCTTTCTTTCTTTTTTTTTTGTAACCTTTAATTTTAGAAAATAGCTTTGGATTTTAGTCACGCCCCTCTGTGATAGAGTGTTTATCATAATATTTCTCAGAATGCTAACTCAAGATCAAGGAAAACTAGGGGGTGGCATGAGGAAATGTCTATTTCGAAAGGAAAAAAAAACAGACCCTCGAGTTTTGAGTTGACTAATTGAGCTGAAAGAAACAATCAAGTCAAGCTCAGTCTTGATGCTCTGGAGCCTCGAGGGTCGTAATCCTCCCTACATCGTCGGTACCACATTTAAGTAACCCTATTAGACAATTCCAAAGGGAAGGAAACAAACTCTAAATAAGGATTTCAATTGTACTTGAACAATGCACTTTCTACACTAACTTAAACATAGAGCTTGGTTGGTTCGATATTACACCGGTGCAAGTACTCTCACACAGGTCAATTTGGAAGACGAGATCAGAGAAGCAGGCCAACCAAGAGTGCACAAAGAGGTCCACGACATGTACACCAACAGTTAATATTACTAATAAAATCTAACTCAAGTCAAACTAGTTAAGTCATATTTTTTTTGATTAAGAGATTAGATATTTTAAGCTTGGTTTCAACCAACTTGTTACACTAATCAGTGGTTTAGTTATTTGTGGAAGTCACATGGGGCGGGAGGTGAGAGGAAGTTTCGTAGGTAAAAGGTAGGGAAAAGGTGAGGAAATGAGTTTTTGATATGTCATATATGTGTGTTGTATTAGGATTGGGTTGAATTAGGGGGATTGACAAGAAAAGAACAACAAGGGTGTTTGGAGGGAGTTGGGTTGTTAACAGCTGTGGATAATAATAATAACAACTATAAATAAATAAATAAATAGAACAACATTTTAAACAATAATATTTACAATAATAGTAATAATAATAGTAATGTATTTATTTAAAGCAAGACAGCCGCATGAGAGATGGGGATTTTAGAGCTTTTGGCGTTGTCCTGCACTGGCCCAACACAACTACAACAAAGGCTAAATCCCATCTCTAACCTTTAAGCTCTGGCTGCTCGGCTGAGCTTCCATACTTTTACTGGCTATTGAATGTCGACATTTCTATCCATTTTTTTTCTTCTCATATATTCACTGATTTAAAACGCACAATAGATGTTTCGATATTGTAAAAAACGAATGAAACACAAGAAAAGATGATATTTAGATCTTGATCATATTTGTATACATATGTGTTCGAACATATCACTACATCGTGATGTTTTATACCTAG

mRNA sequence

ATGAAGATTCTTGTTACTGGGGCATCTGGGTATCTAGGCGGAAGGCTTTGCCGTGCATTATTGAATCGAGGCTTCTCCGTTAGGGCTTTGGTCCGTCCGACCAGCGACCTTTCTTCTCTTCCACATGATCCTTCTGCTCTTGAGCTCGTTCATGGCGATATCACCGATTACCAGTCGCTTCTAGAGGCATGCTCGGGCTGCCATGTCGTTTTCCACGCGGCCGCAATGGTCGAGCCGTGGCTTCCTGATCCATCGAAATTCATTTCTGTCAATGTACGAGGGTTGCAGAACGTGTTGCAAGCGGTAAGAGAGACGAAGACAATCGAGAAAATTATTTATACGTCGTCCTTTTTGCGCTTGGTTCATCACGAGAAGTTCTTTTGTACAGAGTACGAGAAATCGAAGGCCATTGCTGACAAGATTGCATTGCAAGCAGCATCTGAGGGGATTCCGATAGTGCCGGTTTATCCCGGAGTCATCTATGGTGTTGGAAAAGTCACGGCTGGGAATGTTGTTGCTCGCATGCTGATTGAACGGTTCAATGGGCGATTACCCGGATATTTAGGCCAAGGAAACGACAAGTTTTCCTTTAGCCACATCGATGACGTTGTAGAAGGGCATATTGCAGTGATGCAGAAAGGTCGAGTAGGTGAGAGGTATCTTCTAACAGGAGAAAATGCATCATTTGTAGAAGTTTTTGATACGGCTGCAGTCATTACTGGTACGAAGAAACCAATCTTTAATATTCCATTGTGGTTAATTGAAACCTATGGCTGGGTTTCAGTTTTCATCTCTCGAATCACAGGGAAACTTCCTCTCATCAGCCCCCCGACTGTGAAGGTTTTGAGACACCAGTGGGCATATTCGTGTGAGAAAGCAAAACAAGAGCTGGATTATAACCCTAGAAGCTTAAAAGAAGGAATAGAAGAAATGCTAATCTTGATCATATTTGTATACATATGTGTTCGAACATATCACTACATCGTGATGTTTTATACCTAG

Coding sequence (CDS)

ATGAAGATTCTTGTTACTGGGGCATCTGGGTATCTAGGCGGAAGGCTTTGCCGTGCATTATTGAATCGAGGCTTCTCCGTTAGGGCTTTGGTCCGTCCGACCAGCGACCTTTCTTCTCTTCCACATGATCCTTCTGCTCTTGAGCTCGTTCATGGCGATATCACCGATTACCAGTCGCTTCTAGAGGCATGCTCGGGCTGCCATGTCGTTTTCCACGCGGCCGCAATGGTCGAGCCGTGGCTTCCTGATCCATCGAAATTCATTTCTGTCAATGTACGAGGGTTGCAGAACGTGTTGCAAGCGGTAAGAGAGACGAAGACAATCGAGAAAATTATTTATACGTCGTCCTTTTTGCGCTTGGTTCATCACGAGAAGTTCTTTTGTACAGAGTACGAGAAATCGAAGGCCATTGCTGACAAGATTGCATTGCAAGCAGCATCTGAGGGGATTCCGATAGTGCCGGTTTATCCCGGAGTCATCTATGGTGTTGGAAAAGTCACGGCTGGGAATGTTGTTGCTCGCATGCTGATTGAACGGTTCAATGGGCGATTACCCGGATATTTAGGCCAAGGAAACGACAAGTTTTCCTTTAGCCACATCGATGACGTTGTAGAAGGGCATATTGCAGTGATGCAGAAAGGTCGAGTAGGTGAGAGGTATCTTCTAACAGGAGAAAATGCATCATTTGTAGAAGTTTTTGATACGGCTGCAGTCATTACTGGTACGAAGAAACCAATCTTTAATATTCCATTGTGGTTAATTGAAACCTATGGCTGGGTTTCAGTTTTCATCTCTCGAATCACAGGGAAACTTCCTCTCATCAGCCCCCCGACTGTGAAGGTTTTGAGACACCAGTGGGCATATTCGTGTGAGAAAGCAAAACAAGAGCTGGATTATAACCCTAGAAGCTTAAAAGAAGGAATAGAAGAAATGCTAATCTTGATCATATTTGTATACATATGTGTTCGAACATATCACTACATCGTGATGTTTTATACCTAG

Protein sequence

MKILVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLSSLPHDPSALELVHGDITDYQSLLEACSGCHVVFHAAAMVEPWLPDPSKFISVNVRGLQNVLQAVRETKTIEKIIYTSSFLRLVHHEKFFCTEYEKSKAIADKIALQAASEGIPIVPVYPGVIYGVGKVTAGNVVARMLIERFNGRLPGYLGQGNDKFSFSHIDDVVEGHIAVMQKGRVGERYLLTGENASFVEVFDTAAVITGTKKPIFNIPLWLIETYGWVSVFISRITGKLPLISPPTVKVLRHQWAYSCEKAKQELDYNPRSLKEGIEEMLILIIFVYICVRTYHYIVMFYT
Homology
[Close]
BLAST of PI0000209 vs. ExPASy Swiss-Prot
Match: P73212 (Putative dihydroflavonol 4-reductase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=dfrA PE=3 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 1.6e-31
Identity = 102/326 (31.29%), Postives = 153/326 (46.93%), Query Frame = 0 

Query: 5   VTGASGYLGGRLCRALLNRGFSVRALVRPTSDLSSLPHDPSALELVHGDITDYQSLLEAC 64
           VTG +G++G  L R LL +G+ VRALVR +S   +L + P  ++ V GD+ D   L +  
Sbjct: 15  VTGGTGFVGANLVRHLLEQGYQVRALVRASSRPDNLQNLP--IDWVVGDLND-GDLHQQM 74

Query: 65  SGCHVVFHAAAMVEPWLPDPSKFISVNVRGLQNVLQAVRETKTIEKIIYTSSFLRL-VHH 124
            GC  +FH AA    W  D       NV G +N+L A  +   IE+ +YTSS   + V  
Sbjct: 75  QGCQGLFHVAAHYSLWQKDREALYRSNVLGTRNIL-ACAQKAGIERTVYTSSVAAIGVKG 134

Query: 125 EKFFCTE------------YEKSKAIADKIALQAASEGIPIVPVYPGVIYG---VGKVTA 184
           +     E            Y++SK  A++ AL AA +G  IV V P    G   +     
Sbjct: 135 DGQRADESYQSPVEKLIGAYKQSKYWAEQEALTAAQQGQDIVIVNPSTPIGPWDIKPTPT 194

Query: 185 GNVVARMLIERFNGRLPGYLGQGNDKFSFSHIDDVVEGHIAVMQKGRV----GERYLLTG 244
           G ++ R L      ++P Y+  G    +   + DV  GH+   Q+G+     G+RY+L  
Sbjct: 195 GEIILRFL----RRQMPAYVNTG---LNLIDVRDVAAGHLLAWQRGKTALTRGDRYILGH 254

Query: 245 ENASFVEVFDTAAVITGTKKPIFNIPLWLIETYGWVSVFISRITGKLPLISPPTVKVLRH 304
           EN S   +    + ITG   P   +PLWL  T+ WV   +    G+ P +    VK+   
Sbjct: 255 ENISLQGILAHLSTITGLPAPKNTVPLWLPLTFAWVEEKVLAPLGRSPSVPMDGVKMSAQ 314

Query: 305 QWAYSCEKAKQELDYNPRSLKEGIEE 311
           +  Y   KA QEL     S+K+ + +
Sbjct: 315 EMYYDASKAVQELGLPQSSIKQALAD 329

[Close]
BLAST of PI0000209 vs. ExPASy Swiss-Prot
Match: P51103 (Dihydroflavonol 4-reductase OS=Callistephus chinensis OX=13379 GN=F PE=2 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 1.7e-20
Identity = 93/355 (26.20%), Postives = 161/355 (45.35%), Query Frame = 0 

Query: 3   ILVTGASGYLGGRLCRALLNRGFSVRALVRPTSD------LSSLPHDPSALELVHGDITD 62
           + VTGA+G++G  L   LL RG+ VRA VR   D      L  LP   + L L   D+T 
Sbjct: 9   VCVTGAAGFIGSWLVMRLLERGYIVRATVRNPGDMKKVKHLLELPKAETNLTLWKADLTQ 68

Query: 63  YQSLLEACSGCHVVFHAAAMVEPWLPDP-SKFISVNVRGLQNVLQAVRETKTIEKIIYTS 122
             S  EA  GCH VFH A  ++    DP ++ I   + G+ +++++  + KT++K++YTS
Sbjct: 69  EGSFDEAIEGCHGVFHVATPMDFESKDPENEIIKPTIEGILSIIRSCAKAKTVKKLVYTS 128

Query: 123 SF--------------------LRLVHHEKFFCTEYEKSKAIADKIALQAASE-GIPIVP 182
           S                     L  ++ +K     Y  SK +A+K A++AA E  I  V 
Sbjct: 129 SAGTVNVQETQLPVYDESHWSDLDFIYSKKMTAWMYFVSKTLAEKAAMEAAKENNIDFVS 188

Query: 183 VYPGVIYG--VGKVTAGNVVARMLIERFNGRLPGY--LGQGNDKFSFSHIDDVVEGHIAV 242
           + P ++ G  +      +++  + +   NG    Y  + QG     + H+DD+ E HI +
Sbjct: 189 IIPPLVVGPFINPTFPPSLITALSL--INGAESHYSIIKQG----QYVHLDDLCECHIFL 248

Query: 243 MQKGRVGERYLLTGENASFVEVFDTAAVITGTKKPIFNIPLWLIETYGWVSVFISRITGK 302
            +      RY+ + ++A+  ++    A +   K P +++P              + I  +
Sbjct: 249 YENPEAKGRYICSKQDATIHQL----ARMIKQKWPEYHVP-----------TQFAGIDEE 308

Query: 303 LPLISPPTVKV--LRHQWAYSCE----------KAKQELDYNPRSLKEGIEEMLI 314
           LP +S  + K+  +  ++ Y  E          K K  L Y+   +K+G+ E  I
Sbjct: 309 LPTVSFSSKKLIDMGFKFKYDLEDMFKGAIDSCKEKGFLPYSTNEVKKGLFESSI 342

[Close]
BLAST of PI0000209 vs. ExPASy Swiss-Prot
Match: H1ZZB0 (Aurachin B dehydrogenase OS=Stigmatella aurantiaca OX=41 GN=auaH PE=1 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 4.9e-20
Identity = 90/319 (28.21%), Postives = 143/319 (44.83%), Query Frame = 0 

Query: 1   MKILVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLSSLPHDPSALELVHGDITDYQSL 60
           M+  VTG SGYLG  L  AL+ RG SVRALVR  S+ ++        + + G +   ++L
Sbjct: 1   MRTFVTGGSGYLGRNLLSALVARGISVRALVR--SEEAAQKVQALGAQPILGTLEHRETL 60

Query: 61  LEACSGCHVVFHAAAMVEPWLPDPSKFISVNVRGLQNVLQAVRETKTIEKIIYTSSFLRL 120
            E  +GC V+FHAAA+      D ++F   NV G + VL A R+ + I+++++ S+   L
Sbjct: 61  KEGMAGCDVLFHAAALTSARATD-AEFHRANVLGTETVLAAARDAR-IQRMVHVSTEAVL 120

Query: 121 VHHEKFFCTE------------YEKSKAIADKIALQAASEGIPIVPVYPGVIYGVGKVTA 180
                    +            Y  +KA A+++ LQA   G   V V P  I+G      
Sbjct: 121 ADGRPLLQVDESHPLPKRPFAGYPATKAQAEQLVLQANGPGFTTVVVRPRFIWGAD---- 180

Query: 181 GNVVARMLIERFNGRLPGYLGQGNDKFSFSHIDDVVEGHIAVMQKGRVGERYLLTGENAS 240
                  LI+    +   ++  G    S  H+ +V EG +   ++G  GE Y LT     
Sbjct: 181 DTAFLPQLIDAIRTKRFRWVDGGRYLTSTCHVANVCEGMLLAAERGPGGEVYFLTDGAPV 240

Query: 241 FVEVFDTAAVIT-GTKKPIFNIPLWLIETYGWVSVFISRITGKLPLISPPTVK----VLR 300
            +  F T  + T G K  + NIP         +   + R    +P    P ++    +L 
Sbjct: 241 ELRSFLTLLLETQGIKAEVGNIPFQAARAAAHLGESLWR--ALVPQARAPALRLAVYLLG 300

Query: 301 HQWAYSCEKAKQELDYNPR 303
            +   + +KA++EL Y  R
Sbjct: 301 REVTLNDDKARRELGYAGR 309

[Close]
BLAST of PI0000209 vs. ExPASy Swiss-Prot
Match: P51105 (Dihydroflavonol 4-reductase OS=Gerbera hybrida OX=18101 GN=DFR PE=2 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 4.6e-18
Identity = 74/278 (26.62%), Postives = 127/278 (45.68%), Query Frame = 0 

Query: 3   ILVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLS------SLPHDPSALELVHGDITD 62
           + VTGA+G++G  L   LL RG+ V A VR   DL        LP   + L+L   D+T 
Sbjct: 9   VCVTGAAGFIGSWLVMRLLERGYVVHATVRDPGDLKKVKHLLELPKAQTNLKLWKADLTQ 68

Query: 63  YQSLLEACSGCHVVFHAAAMVEPWLPDP-SKFISVNVRGLQNVLQAVRETKTIEKIIYTS 122
             S  EA  GCH VFH A  ++    DP ++ I   + G+ +++++  + KT++K+++TS
Sbjct: 69  EGSFDEAIQGCHGVFHLATPMDFESKDPENEIIKPTIEGVLSIIRSCVKAKTVKKLVFTS 128

Query: 123 SF--------------------LRLVHHEKFFCTEYEKSKAIADKIALQAA-SEGIPIVP 182
           S                     L  ++ +K     Y  SK +A+K A  A     I  + 
Sbjct: 129 SAGTVNGQEKQLHVYDESHWSDLDFIYSKKMTAWMYFVSKTLAEKAAWDATKGNNISFIS 188

Query: 183 VYPGVIYG--VGKVTAGNVVARMLIERFNGRLPGYLGQGNDKFSFSHIDDVVEGHIAVMQ 242
           + P ++ G  +      ++V  + +   N      + QG     + H+DD+ E HI + +
Sbjct: 189 IIPTLVVGPFITSTFPPSLVTALSLITGNEAHYSIIKQG----QYVHLDDLCECHIYLYE 248

Query: 243 KGRVGERYLLTGENASFVEVFDTAAVITGTKKPIFNIP 251
             +   RY+ +  +A+  ++    A I   K P + IP
Sbjct: 249 NPKAKGRYICSSHDATIHQL----AKIIKDKWPEYYIP 278

[Close]
BLAST of PI0000209 vs. ExPASy Swiss-Prot
Match: P14721 (Dihydroflavonol 4-reductase OS=Antirrhinum majus OX=4151 GN=DFRA PE=2 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 5.9e-18
Identity = 76/276 (27.54%), Postives = 128/276 (46.38%), Query Frame = 0 

Query: 3   ILVTGASGYLGGRLCRALLNRGFSVRALVRPTSD------LSSLPHDPSALELVHGDITD 62
           + VTGA+G++G  L   LL RG++VRA VR   +      L  LP   + L L   D+T 
Sbjct: 20  VCVTGAAGFIGSWLVMRLLERGYTVRATVRDPGNMKKVKHLIELPKADTNLTLWKADMTV 79

Query: 63  YQSLLEACSGCHVVFHAAAMVEPWLPDP-SKFISVNVRGLQNVLQAVRETKTIEKIIYT- 122
             S  EA  GC  VFH A  +E    DP ++ I   + G+ N++++  + KT++K I+T 
Sbjct: 80  EGSFDEAIQGCEGVFHLATSMEFDSVDPENEVIKPTIDGMLNIIKSCVQAKTVKKFIFTT 139

Query: 123 -------------------SSFLRLVHHEKFFCTEYEKSKAIADKIALQAASE-GIPIVP 182
                              SS +  ++ +K     Y  SK +A+K  ++AA E  I  + 
Sbjct: 140 SGGTVNVEEHQKPVYDETDSSDMDFINSKKMTGWMYFVSKILAEKAGMEAAKENNIDFIS 199

Query: 183 VYPGVIYGVGKVTAGNVVARMLIERFNGRLPGYLGQGNDKFSFSHIDDVVEGHIAVMQKG 242
           + P ++ G   +          +    G    Y      +  + H+DD+ EGHI + +  
Sbjct: 200 IIPPLVVGPFIMPTFPPSLITALSPITGNEAHY--SIIKQCQYVHLDDLCEGHIFLFEYP 259

Query: 243 RVGERYLLTGENASFVEVFDTAAVITGTKKPIFNIP 251
           +   RY+ +  +A+   ++D A +IT    P ++IP
Sbjct: 260 KAEGRYICSSHDAT---IYDIAKLIT-ENWPEYHIP 289

[Close]
BLAST of PI0000209 vs. ExPASy TrEMBL
Match: A0A5A7V8B0 (Putative dihydroflavonol-4-reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003510 PE=3 SV=1)

HSP 1 Score: 600.9 bits (1548), Expect = 3.2e-168
Identity = 301/324 (92.90%), Postives = 302/324 (93.21%), Query Frame = 0 

Query: 1   MKILVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLSSLPHDPSALELVHGDITDYQSL 60
           M ILVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLSSLPHDPSALELVHGDITDYQSL
Sbjct: 18  MLILVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLSSLPHDPSALELVHGDITDYQSL 77

Query: 61  LEACSGCHVVFHAAAMVEPWLPDPSKFISVNVRGLQNVLQAVRETKTIEKIIYTSSFLRL 120
           LEAC GCHVVFHAAAMVEPWLPDPSKFISVNVRGLQNVLQAVRETKTIEKIIYTSSF  L
Sbjct: 78  LEACLGCHVVFHAAAMVEPWLPDPSKFISVNVRGLQNVLQAVRETKTIEKIIYTSSFFAL 137

Query: 121 ------------VHHEKFFCTEYEKSKAIADKIALQAASEGIPIVPVYPGVIYGVGKVTA 180
                       VHHEKFFCTEYEKSK  ADKIALQAASEGIPIVPVYPGVIYGVGK TA
Sbjct: 138 GSTDGYVAVENQVHHEKFFCTEYEKSKVNADKIALQAASEGIPIVPVYPGVIYGVGKFTA 197

Query: 181 GNVVARMLIERFNGRLPGYLGQGNDKFSFSHIDDVVEGHIAVMQKGRVGERYLLTGENAS 240
           GNVVARMLIERFNGRLPGYLGQGNDKFSFSHIDDVVEGHIA MQKGRVGERYLLTGENAS
Sbjct: 198 GNVVARMLIERFNGRLPGYLGQGNDKFSFSHIDDVVEGHIAAMQKGRVGERYLLTGENAS 257

Query: 241 FVEVFDTAAVITGTKKPIFNIPLWLIETYGWVSVFISRITGKLPLISPPTVKVLRHQWAY 300
           FVEVFDTAA ITGTKKPIFNIPLWLIETYGWV VFISRITGKLPLISPPTVKVLRHQWAY
Sbjct: 258 FVEVFDTAAAITGTKKPIFNIPLWLIETYGWVLVFISRITGKLPLISPPTVKVLRHQWAY 317

Query: 301 SCEKAKQELDYNPRSLKEGIEEML 313
           SCEKAKQELDYNPRSLKEG+EEML
Sbjct: 318 SCEKAKQELDYNPRSLKEGLEEML 341

[Close]
BLAST of PI0000209 vs. ExPASy TrEMBL
Match: A0A1S3BV78 (putative dihydroflavonol-4-reductase OS=Cucumis melo OX=3656 GN=LOC103493529 PE=3 SV=1)

HSP 1 Score: 600.9 bits (1548), Expect = 3.2e-168
Identity = 301/324 (92.90%), Postives = 302/324 (93.21%), Query Frame = 0 

Query: 1   MKILVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLSSLPHDPSALELVHGDITDYQSL 60
           M ILVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLSSLPHDPSALELVHGDITDYQSL
Sbjct: 18  MLILVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLSSLPHDPSALELVHGDITDYQSL 77

Query: 61  LEACSGCHVVFHAAAMVEPWLPDPSKFISVNVRGLQNVLQAVRETKTIEKIIYTSSFLRL 120
           LEAC GCHVVFHAAAMVEPWLPDPSKFISVNVRGLQNVLQAVRETKTIEKIIYTSSF  L
Sbjct: 78  LEACLGCHVVFHAAAMVEPWLPDPSKFISVNVRGLQNVLQAVRETKTIEKIIYTSSFFAL 137

Query: 121 ------------VHHEKFFCTEYEKSKAIADKIALQAASEGIPIVPVYPGVIYGVGKVTA 180
                       VHHEKFFCTEYEKSK  ADKIALQAASEGIPIVPVYPGVIYGVGK TA
Sbjct: 138 GSTDGYVAVENQVHHEKFFCTEYEKSKVNADKIALQAASEGIPIVPVYPGVIYGVGKFTA 197

Query: 181 GNVVARMLIERFNGRLPGYLGQGNDKFSFSHIDDVVEGHIAVMQKGRVGERYLLTGENAS 240
           GNVVARMLIERFNGRLPGYLGQGNDKFSFSHIDDVVEGHIA MQKGRVGERYLLTGENAS
Sbjct: 198 GNVVARMLIERFNGRLPGYLGQGNDKFSFSHIDDVVEGHIAAMQKGRVGERYLLTGENAS 257

Query: 241 FVEVFDTAAVITGTKKPIFNIPLWLIETYGWVSVFISRITGKLPLISPPTVKVLRHQWAY 300
           FVEVFDTAA ITGTKKPIFNIPLWLIETYGWV VFISRITGKLPLISPPTVKVLRHQWAY
Sbjct: 258 FVEVFDTAAAITGTKKPIFNIPLWLIETYGWVLVFISRITGKLPLISPPTVKVLRHQWAY 317

Query: 301 SCEKAKQELDYNPRSLKEGIEEML 313
           SCEKAKQELDYNPRSLKEG+EEML
Sbjct: 318 SCEKAKQELDYNPRSLKEGLEEML 341

[Close]
BLAST of PI0000209 vs. ExPASy TrEMBL
Match: A0A6J1E109 (uncharacterized protein LOC111026159 OS=Momordica charantia OX=3673 GN=LOC111026159 PE=3 SV=1)

HSP 1 Score: 575.1 bits (1481), Expect = 1.9e-160
Identity = 279/324 (86.11%), Postives = 299/324 (92.28%), Query Frame = 0 

Query: 1   MKILVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLSSLPHDPSALELVHGDITDYQSL 60
           MKILVTGASGYLGGRLCRALLNRG+SVRAL+RPTSDLSSLPHDP+ALELVHGDITDYQSL
Sbjct: 1   MKILVTGASGYLGGRLCRALLNRGYSVRALIRPTSDLSSLPHDPAALELVHGDITDYQSL 60

Query: 61  LEACSGCHVVFHAAAMVEPWLPDPSKFISVNVRGLQNVLQAVRETKTIEKIIYTSSFLRL 120
           LEACSGCHVVFHAAA+VEPWLPDPSKFISVNVRGL+NVLQAV ET+TIEKIIYTSSF  L
Sbjct: 61  LEACSGCHVVFHAAALVEPWLPDPSKFISVNVRGLKNVLQAVTETRTIEKIIYTSSFFAL 120

Query: 121 ------------VHHEKFFCTEYEKSKAIADKIALQAASEGIPIVPVYPGVIYGVGKVTA 180
                       VHHEKFFCTEYEKSKAIADKIAL AASEGIPIVPVYPGVIYG GKVTA
Sbjct: 121 GSTDGYVADENQVHHEKFFCTEYEKSKAIADKIALHAASEGIPIVPVYPGVIYGSGKVTA 180

Query: 181 GNVVARMLIERFNGRLPGYLGQGNDKFSFSHIDDVVEGHIAVMQKGRVGERYLLTGENAS 240
           GNV+ARM+IERFN RLPGYLG+GNDKFSFSH+DDVVEGH+A +Q+GRVGERYLLTGENAS
Sbjct: 181 GNVIARMMIERFNWRLPGYLGRGNDKFSFSHVDDVVEGHLAALQRGRVGERYLLTGENAS 240

Query: 241 FVEVFDTAAVITGTKKPIFNIPLWLIETYGWVSVFISRITGKLPLISPPTVKVLRHQWAY 300
           FV+VFD AA ITGT++PIF IPLWLIE YGWVS+FISRITGKLPLISPPTV  LRHQWAY
Sbjct: 241 FVDVFDIAAAITGTRRPIFKIPLWLIEAYGWVSIFISRITGKLPLISPPTVNPLRHQWAY 300

Query: 301 SCEKAKQELDYNPRSLKEGIEEML 313
           SCEKAK+ELDYNPRSL+EG+EEML
Sbjct: 301 SCEKAKEELDYNPRSLREGLEEML 324

[Close]
BLAST of PI0000209 vs. ExPASy TrEMBL
Match: A0A6J1J4E6 (uncharacterized protein LOC111481147 OS=Cucurbita maxima OX=3661 GN=LOC111481147 PE=3 SV=1)

HSP 1 Score: 568.9 bits (1465), Expect = 1.4e-158
Identity = 275/324 (84.88%), Postives = 299/324 (92.28%), Query Frame = 0 

Query: 1   MKILVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLSSLPHDPSALELVHGDITDYQSL 60
           MK+LVTGASGYLGGRLC ALLNRGFSVRALVRPTSDLSSLPHDPSALELV+GD+T+YQSL
Sbjct: 1   MKVLVTGASGYLGGRLCHALLNRGFSVRALVRPTSDLSSLPHDPSALELVYGDVTNYQSL 60

Query: 61  LEACSGCHVVFHAAAMVEPWLPDPSKFISVNVRGLQNVLQAVRETKTIEKIIYTSSFLRL 120
           L+A SGCH+VFHAAA+VEPW+PDPSKFISVNVRGL+NVLQAV ETKTIEKIIYTSSF  L
Sbjct: 61  LDAFSGCHIVFHAAALVEPWIPDPSKFISVNVRGLKNVLQAVTETKTIEKIIYTSSFFAL 120

Query: 121 ------------VHHEKFFCTEYEKSKAIADKIALQAASEGIPIVPVYPGVIYGVGKVTA 180
                       VHHEKFFCTEYEKSKAIADKIALQAASEG+PIVPVYPGVIYG GK+TA
Sbjct: 121 GSTDGYVADENQVHHEKFFCTEYEKSKAIADKIALQAASEGVPIVPVYPGVIYGSGKLTA 180

Query: 181 GNVVARMLIERFNGRLPGYLGQGNDKFSFSHIDDVVEGHIAVMQKGRVGERYLLTGENAS 240
           GNV+ARMLIERFN RLPGY+GQG D++SFSH+DDVVEGHIA MQKGRVGERYLLTGENAS
Sbjct: 181 GNVIARMLIERFNWRLPGYIGQGTDRYSFSHVDDVVEGHIAAMQKGRVGERYLLTGENAS 240

Query: 241 FVEVFDTAAVITGTKKPIFNIPLWLIETYGWVSVFISRITGKLPLISPPTVKVLRHQWAY 300
           FV+VFDTAA ITGT++P+FNIPLWLIE YGWVSV+ISRITGKLPLISPP V VLRHQWAY
Sbjct: 241 FVDVFDTAAAITGTRRPMFNIPLWLIEAYGWVSVYISRITGKLPLISPPVVSVLRHQWAY 300

Query: 301 SCEKAKQELDYNPRSLKEGIEEML 313
           SCEKAK+ELDYNPRSLKEG+EEM+
Sbjct: 301 SCEKAKEELDYNPRSLKEGLEEMV 324

[Close]
BLAST of PI0000209 vs. ExPASy TrEMBL
Match: A0A6J1FLK2 (uncharacterized protein LOC111446335 OS=Cucurbita moschata OX=3662 GN=LOC111446335 PE=3 SV=1)

HSP 1 Score: 565.1 bits (1455), Expect = 2.0e-157
Identity = 273/324 (84.26%), Postives = 298/324 (91.98%), Query Frame = 0 

Query: 1   MKILVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLSSLPHDPSALELVHGDITDYQSL 60
           MK+LVTGASGYLGGRLC ALLNRGFSVRALVRPTSDLSSLPHDPSALELV+GD+TDYQSL
Sbjct: 1   MKVLVTGASGYLGGRLCHALLNRGFSVRALVRPTSDLSSLPHDPSALELVYGDVTDYQSL 60

Query: 61  LEACSGCHVVFHAAAMVEPWLPDPSKFISVNVRGLQNVLQAVRETKTIEKIIYTSSFLRL 120
           L+A SGCH+VFHAAA+VEPW+PDPSKFISVNVRGL+NVLQAV ETKTIEKIIYTSSF  L
Sbjct: 61  LDAFSGCHIVFHAAALVEPWIPDPSKFISVNVRGLKNVLQAVTETKTIEKIIYTSSFFAL 120

Query: 121 ------------VHHEKFFCTEYEKSKAIADKIALQAASEGIPIVPVYPGVIYGVGKVTA 180
                       VHHEKFFCTEYEKSKAIADKIALQAASEG+PIVPVYPGVIYG GK+TA
Sbjct: 121 GSTDGYVADENQVHHEKFFCTEYEKSKAIADKIALQAASEGVPIVPVYPGVIYGSGKLTA 180

Query: 181 GNVVARMLIERFNGRLPGYLGQGNDKFSFSHIDDVVEGHIAVMQKGRVGERYLLTGENAS 240
           GNV+AR+LIERFN RLPGY+GQG+D++SFSH+DDVVEGHIA MQKGRVGERYLLTGENAS
Sbjct: 181 GNVIARLLIERFNWRLPGYIGQGSDRYSFSHVDDVVEGHIAAMQKGRVGERYLLTGENAS 240

Query: 241 FVEVFDTAAVITGTKKPIFNIPLWLIETYGWVSVFISRITGKLPLISPPTVKVLRHQWAY 300
           FV+VFDTAA ITGT++P+FNIPLWLIE YGWVSV ISRITGKLPLISPP V VLRH+WAY
Sbjct: 241 FVDVFDTAAAITGTRRPMFNIPLWLIEAYGWVSVVISRITGKLPLISPPVVSVLRHEWAY 300

Query: 301 SCEKAKQELDYNPRSLKEGIEEML 313
           SCEKAK+ELDY PRSLKEG+EEM+
Sbjct: 301 SCEKAKEELDYKPRSLKEGLEEMV 324

[Close]
BLAST of PI0000209 vs. NCBI nr
Match: XP_011654104.1 (uncharacterized protein LOC101223178 isoform X1 [Cucumis sativus] >KAE8649859.1 hypothetical protein Csa_012339 [Cucumis sativus])

HSP 1 Score: 606.3 bits (1562), Expect = 1.6e-169
Identity = 302/324 (93.21%), Postives = 304/324 (93.83%), Query Frame = 0 

Query: 1   MKILVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLSSLPHDPSALELVHGDITDYQSL 60
           MKILVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLSSLPHDPSALELVHGDITDYQSL
Sbjct: 1   MKILVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLSSLPHDPSALELVHGDITDYQSL 60

Query: 61  LEACSGCHVVFHAAAMVEPWLPDPSKFISVNVRGLQNVLQAVRETKTIEKIIYTSSFLRL 120
           LEACSGCHVVFHAAAMVEPWLPDPSKFISVNVRGLQNVLQAVRETKTIEKIIYTSSF  L
Sbjct: 61  LEACSGCHVVFHAAAMVEPWLPDPSKFISVNVRGLQNVLQAVRETKTIEKIIYTSSFFAL 120

Query: 121 ------------VHHEKFFCTEYEKSKAIADKIALQAASEGIPIVPVYPGVIYGVGKVTA 180
                       VHHEKFFCTEYEKSKA ADKIALQAASEGIPIVPVYPGVIYGVGKVTA
Sbjct: 121 GSTDGYVAVESQVHHEKFFCTEYEKSKATADKIALQAASEGIPIVPVYPGVIYGVGKVTA 180

Query: 181 GNVVARMLIERFNGRLPGYLGQGNDKFSFSHIDDVVEGHIAVMQKGRVGERYLLTGENAS 240
           GNVVARMLIERFNGRLPGYLGQG DKFSFSH+DDVVEGHI  MQKGRVGERYLLTGENAS
Sbjct: 181 GNVVARMLIERFNGRLPGYLGQGKDKFSFSHVDDVVEGHIVAMQKGRVGERYLLTGENAS 240

Query: 241 FVEVFDTAAVITGTKKPIFNIPLWLIETYGWVSVFISRITGKLPLISPPTVKVLRHQWAY 300
           FVEVFD AA ITGTKKPIFNIPLWLIETYGWVSVFISRITGKLPLISPPTVKVLRHQWAY
Sbjct: 241 FVEVFDAAAAITGTKKPIFNIPLWLIETYGWVSVFISRITGKLPLISPPTVKVLRHQWAY 300

Query: 301 SCEKAKQELDYNPRSLKEGIEEML 313
           SCEKAKQELDYNPRSLKEG+EEML
Sbjct: 301 SCEKAKQELDYNPRSLKEGLEEML 324

[Close]
BLAST of PI0000209 vs. NCBI nr
Match: XP_008452530.1 (PREDICTED: putative dihydroflavonol-4-reductase [Cucumis melo] >KAA0064373.1 putative dihydroflavonol-4-reductase [Cucumis melo var. makuwa] >TYK20214.1 putative dihydroflavonol-4-reductase [Cucumis melo var. makuwa])

HSP 1 Score: 600.9 bits (1548), Expect = 6.7e-168
Identity = 301/324 (92.90%), Postives = 302/324 (93.21%), Query Frame = 0 

Query: 1   MKILVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLSSLPHDPSALELVHGDITDYQSL 60
           M ILVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLSSLPHDPSALELVHGDITDYQSL
Sbjct: 18  MLILVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLSSLPHDPSALELVHGDITDYQSL 77

Query: 61  LEACSGCHVVFHAAAMVEPWLPDPSKFISVNVRGLQNVLQAVRETKTIEKIIYTSSFLRL 120
           LEAC GCHVVFHAAAMVEPWLPDPSKFISVNVRGLQNVLQAVRETKTIEKIIYTSSF  L
Sbjct: 78  LEACLGCHVVFHAAAMVEPWLPDPSKFISVNVRGLQNVLQAVRETKTIEKIIYTSSFFAL 137

Query: 121 ------------VHHEKFFCTEYEKSKAIADKIALQAASEGIPIVPVYPGVIYGVGKVTA 180
                       VHHEKFFCTEYEKSK  ADKIALQAASEGIPIVPVYPGVIYGVGK TA
Sbjct: 138 GSTDGYVAVENQVHHEKFFCTEYEKSKVNADKIALQAASEGIPIVPVYPGVIYGVGKFTA 197

Query: 181 GNVVARMLIERFNGRLPGYLGQGNDKFSFSHIDDVVEGHIAVMQKGRVGERYLLTGENAS 240
           GNVVARMLIERFNGRLPGYLGQGNDKFSFSHIDDVVEGHIA MQKGRVGERYLLTGENAS
Sbjct: 198 GNVVARMLIERFNGRLPGYLGQGNDKFSFSHIDDVVEGHIAAMQKGRVGERYLLTGENAS 257

Query: 241 FVEVFDTAAVITGTKKPIFNIPLWLIETYGWVSVFISRITGKLPLISPPTVKVLRHQWAY 300
           FVEVFDTAA ITGTKKPIFNIPLWLIETYGWV VFISRITGKLPLISPPTVKVLRHQWAY
Sbjct: 258 FVEVFDTAAAITGTKKPIFNIPLWLIETYGWVLVFISRITGKLPLISPPTVKVLRHQWAY 317

Query: 301 SCEKAKQELDYNPRSLKEGIEEML 313
           SCEKAKQELDYNPRSLKEG+EEML
Sbjct: 318 SCEKAKQELDYNPRSLKEGLEEML 341

[Close]
BLAST of PI0000209 vs. NCBI nr
Match: XP_038898413.1 (putative dihydroflavonol 4-reductase [Benincasa hispida])

HSP 1 Score: 595.1 bits (1533), Expect = 3.7e-166
Identity = 292/324 (90.12%), Postives = 304/324 (93.83%), Query Frame = 0 

Query: 1   MKILVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLSSLPHDPSALELVHGDITDYQSL 60
           MK+L+TGASGYLGGRLCRALL+RGFSVRALVRPTSDLSSLPHDPSALELVHGDITDYQSL
Sbjct: 1   MKVLLTGASGYLGGRLCRALLDRGFSVRALVRPTSDLSSLPHDPSALELVHGDITDYQSL 60

Query: 61  LEACSGCHVVFHAAAMVEPWLPDPSKFISVNVRGLQNVLQAVRETKTIEKIIYTSSFLRL 120
           LEAC GCHVVFHAAAMVEPWLPDPSKFISVNVRGL+NVLQAVR+TKTIEKIIYTSSF  L
Sbjct: 61  LEACWGCHVVFHAAAMVEPWLPDPSKFISVNVRGLENVLQAVRKTKTIEKIIYTSSFFAL 120

Query: 121 ------------VHHEKFFCTEYEKSKAIADKIALQAASEGIPIVPVYPGVIYGVGKVTA 180
                       VHHEKFFCTEYEKSKAIADKIALQAASEGIPIVPVYPGVIYG GKVTA
Sbjct: 121 GSTDGYVADEKQVHHEKFFCTEYEKSKAIADKIALQAASEGIPIVPVYPGVIYGAGKVTA 180

Query: 181 GNVVARMLIERFNGRLPGYLGQGNDKFSFSHIDDVVEGHIAVMQKGRVGERYLLTGENAS 240
           GNV+ARMLIERFNGRLPGYLGQGNDK+SFSH+DDVVEGHIA MQKGRVGERYLLTGENAS
Sbjct: 181 GNVIARMLIERFNGRLPGYLGQGNDKYSFSHVDDVVEGHIAAMQKGRVGERYLLTGENAS 240

Query: 241 FVEVFDTAAVITGTKKPIFNIPLWLIETYGWVSVFISRITGKLPLISPPTVKVLRHQWAY 300
           FVEVFDTAA ITGT+KP+FNIPLW IE YGWVSVFISRITGKLPLISPPTVKVLRHQWAY
Sbjct: 241 FVEVFDTAAAITGTRKPMFNIPLWFIEAYGWVSVFISRITGKLPLISPPTVKVLRHQWAY 300

Query: 301 SCEKAKQELDYNPRSLKEGIEEML 313
           SC+KAKQELDYNPRSLKEG+EEML
Sbjct: 301 SCDKAKQELDYNPRSLKEGLEEML 324

[Close]
BLAST of PI0000209 vs. NCBI nr
Match: XP_022159877.1 (uncharacterized protein LOC111026159 [Momordica charantia])

HSP 1 Score: 575.1 bits (1481), Expect = 3.9e-160
Identity = 279/324 (86.11%), Postives = 299/324 (92.28%), Query Frame = 0 

Query: 1   MKILVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLSSLPHDPSALELVHGDITDYQSL 60
           MKILVTGASGYLGGRLCRALLNRG+SVRAL+RPTSDLSSLPHDP+ALELVHGDITDYQSL
Sbjct: 1   MKILVTGASGYLGGRLCRALLNRGYSVRALIRPTSDLSSLPHDPAALELVHGDITDYQSL 60

Query: 61  LEACSGCHVVFHAAAMVEPWLPDPSKFISVNVRGLQNVLQAVRETKTIEKIIYTSSFLRL 120
           LEACSGCHVVFHAAA+VEPWLPDPSKFISVNVRGL+NVLQAV ET+TIEKIIYTSSF  L
Sbjct: 61  LEACSGCHVVFHAAALVEPWLPDPSKFISVNVRGLKNVLQAVTETRTIEKIIYTSSFFAL 120

Query: 121 ------------VHHEKFFCTEYEKSKAIADKIALQAASEGIPIVPVYPGVIYGVGKVTA 180
                       VHHEKFFCTEYEKSKAIADKIAL AASEGIPIVPVYPGVIYG GKVTA
Sbjct: 121 GSTDGYVADENQVHHEKFFCTEYEKSKAIADKIALHAASEGIPIVPVYPGVIYGSGKVTA 180

Query: 181 GNVVARMLIERFNGRLPGYLGQGNDKFSFSHIDDVVEGHIAVMQKGRVGERYLLTGENAS 240
           GNV+ARM+IERFN RLPGYLG+GNDKFSFSH+DDVVEGH+A +Q+GRVGERYLLTGENAS
Sbjct: 181 GNVIARMMIERFNWRLPGYLGRGNDKFSFSHVDDVVEGHLAALQRGRVGERYLLTGENAS 240

Query: 241 FVEVFDTAAVITGTKKPIFNIPLWLIETYGWVSVFISRITGKLPLISPPTVKVLRHQWAY 300
           FV+VFD AA ITGT++PIF IPLWLIE YGWVS+FISRITGKLPLISPPTV  LRHQWAY
Sbjct: 241 FVDVFDIAAAITGTRRPIFKIPLWLIEAYGWVSIFISRITGKLPLISPPTVNPLRHQWAY 300

Query: 301 SCEKAKQELDYNPRSLKEGIEEML 313
           SCEKAK+ELDYNPRSL+EG+EEML
Sbjct: 301 SCEKAKEELDYNPRSLREGLEEML 324

[Close]
BLAST of PI0000209 vs. NCBI nr
Match: XP_023525575.1 (uncharacterized protein LOC111789148 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 572.4 bits (1474), Expect = 2.6e-159
Identity = 277/324 (85.49%), Postives = 300/324 (92.59%), Query Frame = 0 

Query: 1   MKILVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLSSLPHDPSALELVHGDITDYQSL 60
           MK+LVTGASGYLGGRLC ALLNRGFSVRALVRPTSDLSSLPHDPSALELV+GD+TDYQSL
Sbjct: 1   MKVLVTGASGYLGGRLCHALLNRGFSVRALVRPTSDLSSLPHDPSALELVYGDVTDYQSL 60

Query: 61  LEACSGCHVVFHAAAMVEPWLPDPSKFISVNVRGLQNVLQAVRETKTIEKIIYTSSFLRL 120
           L+A SGCH+VFHAAA+VEPW+PDPSKFISVNVRGL+NVLQAV ETKTIEKIIYTSSF  L
Sbjct: 61  LDAFSGCHIVFHAAALVEPWIPDPSKFISVNVRGLKNVLQAVTETKTIEKIIYTSSFFAL 120

Query: 121 ------------VHHEKFFCTEYEKSKAIADKIALQAASEGIPIVPVYPGVIYGVGKVTA 180
                       VHHEKFFCTEYEKSKAIADKIALQAASEG+PIVPVYPGVIYG GK+TA
Sbjct: 121 GSTDGYVADENQVHHEKFFCTEYEKSKAIADKIALQAASEGVPIVPVYPGVIYGSGKLTA 180

Query: 181 GNVVARMLIERFNGRLPGYLGQGNDKFSFSHIDDVVEGHIAVMQKGRVGERYLLTGENAS 240
           GNV+ARMLIERFN RLPGY+GQG+D++SFSH+DDVVEGHIA MQKGRVGERYLLTGENAS
Sbjct: 181 GNVIARMLIERFNWRLPGYIGQGSDRYSFSHVDDVVEGHIAAMQKGRVGERYLLTGENAS 240

Query: 241 FVEVFDTAAVITGTKKPIFNIPLWLIETYGWVSVFISRITGKLPLISPPTVKVLRHQWAY 300
           FV+VFDTAA ITGT++P+FNIPLWLIE YGWVSVFISRITGKLPLISPP V VLRHQWAY
Sbjct: 241 FVDVFDTAAAITGTRRPMFNIPLWLIEAYGWVSVFISRITGKLPLISPPVVSVLRHQWAY 300

Query: 301 SCEKAKQELDYNPRSLKEGIEEML 313
           SCEKAK+ELDYNPRSLKEG+EEM+
Sbjct: 301 SCEKAKEELDYNPRSLKEGLEEMV 324

[Close]
BLAST of PI0000209 vs. TAIR 10
Match: AT4G33360.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 489.6 bits (1259), Expect = 2.0e-138
Identity = 235/324 (72.53%), Postives = 277/324 (85.49%), Query Frame = 0 

Query: 1   MKILVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLSSLPHDPSALELVHGDITDYQSL 60
           MKILVTG++GYLG RLC  LL RG SVRALVR TSDLS LP +   +EL +GD+TDY+SL
Sbjct: 13  MKILVTGSTGYLGARLCHVLLRRGHSVRALVRRTSDLSDLPPE---VELAYGDVTDYRSL 72

Query: 61  LEACSGCHVVFHAAAMVEPWLPDPSKFISVNVRGLQNVLQAVRETKTIEKIIYTSSFLRL 120
            +ACSGC +VFHAAA+VEPWLPDPS+FISVNV GL+NVL+AV+ETKT++KIIYTSSF  L
Sbjct: 73  TDACSGCDIVFHAAALVEPWLPDPSRFISVNVGGLKNVLEAVKETKTVQKIIYTSSFFAL 132

Query: 121 ------------VHHEKFFCTEYEKSKAIADKIALQAASEGIPIVPVYPGVIYGVGKVTA 180
                       VH+E+FFCTEYE+SKA+ADK+AL AASEG+PI+ +YPGVI+G GK+T+
Sbjct: 133 GSTDGSVANENQVHNERFFCTEYERSKAVADKMALNAASEGVPIILLYPGVIFGPGKLTS 192

Query: 181 GNVVARMLIERFNGRLPGYLGQGNDKFSFSHIDDVVEGHIAVMQKGRVGERYLLTGENAS 240
            N+VARMLIERFNGRLPGY+G G D++SFSH+DDVVEGH+A M+KGR+GERYLLTGENAS
Sbjct: 193 ANMVARMLIERFNGRLPGYIGSGTDRYSFSHVDDVVEGHVAAMEKGRLGERYLLTGENAS 252

Query: 241 FVEVFDTAAVITGTKKPIFNIPLWLIETYGWVSVFISRITGKLPLISPPTVKVLRHQWAY 300
           F  VFD AA+ITGTKKP F+IPLW I  YGW+SV ISR+TGKLPLISPPTV VLRHQW+Y
Sbjct: 253 FKLVFDMAALITGTKKPNFSIPLWAINAYGWLSVLISRVTGKLPLISPPTVTVLRHQWSY 312

Query: 301 SCEKAKQELDYNPRSLKEGIEEML 313
           SC+KAK EL YNPRSLKEG+EEML
Sbjct: 313 SCDKAKLELGYNPRSLKEGLEEML 333

[Close]
BLAST of PI0000209 vs. TAIR 10
Match: AT4G33360.3 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 489.6 bits (1259), Expect = 2.0e-138
Identity = 235/324 (72.53%), Postives = 277/324 (85.49%), Query Frame = 0 

Query: 1   MKILVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLSSLPHDPSALELVHGDITDYQSL 60
           MKILVTG++GYLG RLC  LL RG SVRALVR TSDLS LP +   +EL +GD+TDY+SL
Sbjct: 1   MKILVTGSTGYLGARLCHVLLRRGHSVRALVRRTSDLSDLPPE---VELAYGDVTDYRSL 60

Query: 61  LEACSGCHVVFHAAAMVEPWLPDPSKFISVNVRGLQNVLQAVRETKTIEKIIYTSSFLRL 120
            +ACSGC +VFHAAA+VEPWLPDPS+FISVNV GL+NVL+AV+ETKT++KIIYTSSF  L
Sbjct: 61  TDACSGCDIVFHAAALVEPWLPDPSRFISVNVGGLKNVLEAVKETKTVQKIIYTSSFFAL 120

Query: 121 ------------VHHEKFFCTEYEKSKAIADKIALQAASEGIPIVPVYPGVIYGVGKVTA 180
                       VH+E+FFCTEYE+SKA+ADK+AL AASEG+PI+ +YPGVI+G GK+T+
Sbjct: 121 GSTDGSVANENQVHNERFFCTEYERSKAVADKMALNAASEGVPIILLYPGVIFGPGKLTS 180

Query: 181 GNVVARMLIERFNGRLPGYLGQGNDKFSFSHIDDVVEGHIAVMQKGRVGERYLLTGENAS 240
            N+VARMLIERFNGRLPGY+G G D++SFSH+DDVVEGH+A M+KGR+GERYLLTGENAS
Sbjct: 181 ANMVARMLIERFNGRLPGYIGSGTDRYSFSHVDDVVEGHVAAMEKGRLGERYLLTGENAS 240

Query: 241 FVEVFDTAAVITGTKKPIFNIPLWLIETYGWVSVFISRITGKLPLISPPTVKVLRHQWAY 300
           F  VFD AA+ITGTKKP F+IPLW I  YGW+SV ISR+TGKLPLISPPTV VLRHQW+Y
Sbjct: 241 FKLVFDMAALITGTKKPNFSIPLWAINAYGWLSVLISRVTGKLPLISPPTVTVLRHQWSY 300

Query: 301 SCEKAKQELDYNPRSLKEGIEEML 313
           SC+KAK EL YNPRSLKEG+EEML
Sbjct: 301 SCDKAKLELGYNPRSLKEGLEEML 321

[Close]
BLAST of PI0000209 vs. TAIR 10
Match: AT4G33360.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 427.6 bits (1098), Expect = 9.5e-120
Identity = 206/289 (71.28%), Postives = 245/289 (84.78%), Query Frame = 0 

Query: 1   MKILVTGASGYLGGRLCRALLNRGFSVRALVRPTSDLSSLPHDPSALELVHGDITDYQSL 60
           MKILVTG++GYLG RLC  LL RG SVRALVR TSDLS LP +   +EL +GD+TDY+SL
Sbjct: 13  MKILVTGSTGYLGARLCHVLLRRGHSVRALVRRTSDLSDLPPE---VELAYGDVTDYRSL 72

Query: 61  LEACSGCHVVFHAAAMVEPWLPDPSKFISVNVRGLQNVLQAVRETKTIEKIIYTSSFLRL 120
            +ACSGC +VFHAAA+VEPWLPDPS+FISVNV GL+NVL+AV+ETKT++KIIYTSSF  L
Sbjct: 73  TDACSGCDIVFHAAALVEPWLPDPSRFISVNVGGLKNVLEAVKETKTVQKIIYTSSFFAL 132

Query: 121 ------------VHHEKFFCTEYEKSKAIADKIALQAASEGIPIVPVYPGVIYGVGKVTA 180
                       VH+E+FFCTEYE+SKA+ADK+AL AASEG+PI+ +YPGVI+G GK+T+
Sbjct: 133 GSTDGSVANENQVHNERFFCTEYERSKAVADKMALNAASEGVPIILLYPGVIFGPGKLTS 192

Query: 181 GNVVARMLIERFNGRLPGYLGQGNDKFSFSHIDDVVEGHIAVMQKGRVGERYLLTGENAS 240
            N+VARMLIERFNGRLPGY+G G D++SFSH+DDVVEGH+A M+KGR+GERYLLTGENAS
Sbjct: 193 ANMVARMLIERFNGRLPGYIGSGTDRYSFSHVDDVVEGHVAAMEKGRLGERYLLTGENAS 252

Query: 241 FVEVFDTAAVITGTKKPIFNIPLWLIETYGWVSVFISRITGKLPLISPP 278
           F  VFD AA+ITGTKKP F+IPLW I  YGW+SV ISR+TGKLPLISPP
Sbjct: 253 FKLVFDMAALITGTKKPNFSIPLWAINAYGWLSVLISRVTGKLPLISPP 298

[Close]
BLAST of PI0000209 vs. TAIR 10
Match: AT1G09480.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 99.4 bits (246), Expect = 5.9e-21
Identity = 78/261 (29.89%), Postives = 123/261 (47.13%), Query Frame = 0 

Query: 3   ILVTGASGYLGGRLCRALLNRGFSVRALVRPTSD------LSSLPHDPSALELVHGDITD 62
           + VTGASGY+   + + LL RG++V+A VR  +D      L +L      L+L   D+ +
Sbjct: 55  VCVTGASGYIASWIVKLLLLRGYTVKATVRDLTDRKKTEHLLALDGAKERLKLFKADLLE 114

Query: 63  YQSLLEACSGCHVVFHAAAMVEPWLPDP-SKFISVNVRGLQNVLQAVRETKTIEKIIYTS 122
             S  +A  GC  VFH A+ V   + DP ++ I   ++G  NVL   +ET ++ ++I TS
Sbjct: 115 ESSFEQAIEGCDAVFHTASPVFFTVKDPQTELIDPALKGTMNVLNTCKETPSVRRVILTS 174

Query: 123 SFLRL-----------VHHEKFF-----CTE----YEKSKAIADKIALQAASE-GIPIVP 182
           S   +           V  E FF     C E    Y  SK +A+  A + A + GI +V 
Sbjct: 175 STAAVLFRQPPVEASDVVDETFFSDPSLCRETKNWYPLSKILAENAAWEFAKDNGIDMVV 234

Query: 183 VYPGVIYGVGKVTAGNVVARMLIERFNGRLPGYLGQGNDKF-SFSHIDDVVEGHIAVMQK 235
           + PG I+G       N    ++++  NG+ P      N +F  F  + DV   HI  ++ 
Sbjct: 235 LNPGFIFGPLLQPTLNFSVELIVDFINGKNP-----FNSRFYRFVDVRDVALAHIKALET 294

[Close]
BLAST of PI0000209 vs. TAIR 10
Match: AT1G09490.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 98.2 bits (243), Expect = 1.3e-20
Identity = 76/260 (29.23%), Postives = 121/260 (46.54%), Query Frame = 0 

Query: 3   ILVTGASGYLGGRLCRALLNRGFSVRALVRPTSD------LSSLPHDPSALELVHGDITD 62
           + VTGASGY+   + + LL RG++V A VR   D      L +L      L+L   D+ +
Sbjct: 8   VCVTGASGYIASWIVKLLLLRGYTVNATVRDPKDKKKTEHLLALDGAKERLKLFKADLLE 67

Query: 63  YQSLLEACSGCHVVFHAAAMVEPWLPDP-SKFISVNVRGLQNVLQAVRETKTIEKIIYTS 122
             S  +A  GC  VFH A+ V   + DP ++ I   ++G  NVL   ++  +++++I TS
Sbjct: 68  ESSFDQAIDGCDAVFHTASPVLFTVTDPQTELIDPALKGTINVLNTCKQVSSVKRVILTS 127

Query: 123 SFLRLVHH-----------EKFF-----CTE----YEKSKAIADKIALQAASE-GIPIVP 182
           S   ++             E FF     C E    Y  SK +A+  A Q A + GI +V 
Sbjct: 128 STAAVLSRQPPIGPNDLVDETFFSDPSLCRETKNWYSLSKILAENAAWQFAKDNGIDMVV 187

Query: 183 VYPGVIYGVGKVTAGNVVARMLIERFNGRLPGYLGQGNDKFSFSHIDDVVEGHIAVMQKG 235
           + PG I G       N+   ++++  NG+ P         + FS + DV   HI  ++  
Sbjct: 188 LNPGFICGPLLQPTLNMSVELIVDFINGKNP----FNKRYYRFSDVRDVALVHIKALETP 247

The following BLAST results are available for this feature:
BLAST of PI0000209 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
P732121.6e-3131.29Putative dihydroflavonol 4-reductase OS=Synechocystis sp. (strain PCC 6803 / Kaz... [more]
P511031.7e-2026.20Dihydroflavonol 4-reductase OS=Callistephus chinensis OX=13379 GN=F PE=2 SV=1[more]
H1ZZB04.9e-2028.21Aurachin B dehydrogenase OS=Stigmatella aurantiaca OX=41 GN=auaH PE=1 SV=1[more]
P511054.6e-1826.62Dihydroflavonol 4-reductase OS=Gerbera hybrida OX=18101 GN=DFR PE=2 SV=1[more]
P147215.9e-1827.54Dihydroflavonol 4-reductase OS=Antirrhinum majus OX=4151 GN=DFRA PE=2 SV=1[more]
BLAST of PI0000209 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5A7V8B03.2e-16892.90Putative dihydroflavonol-4-reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3BV783.2e-16892.90putative dihydroflavonol-4-reductase OS=Cucumis melo OX=3656 GN=LOC103493529 PE=... [more]
A0A6J1E1091.9e-16086.11uncharacterized protein LOC111026159 OS=Momordica charantia OX=3673 GN=LOC111026... [more]
A0A6J1J4E61.4e-15884.88uncharacterized protein LOC111481147 OS=Cucurbita maxima OX=3661 GN=LOC111481147... [more]
A0A6J1FLK22.0e-15784.26uncharacterized protein LOC111446335 OS=Cucurbita moschata OX=3662 GN=LOC1114463... [more]
BLAST of PI0000209 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_011654104.11.6e-16993.21uncharacterized protein LOC101223178 isoform X1 [Cucumis sativus] >KAE8649859.1 ... [more]
XP_008452530.16.7e-16892.90PREDICTED: putative dihydroflavonol-4-reductase [Cucumis melo] >KAA0064373.1 put... [more]
XP_038898413.13.7e-16690.12putative dihydroflavonol 4-reductase [Benincasa hispida][more]
XP_022159877.13.9e-16086.11uncharacterized protein LOC111026159 [Momordica charantia][more]
XP_023525575.12.6e-15985.49uncharacterized protein LOC111789148 [Cucurbita pepo subsp. pepo][more]
BLAST of PI0000209 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G33360.12.0e-13872.53NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G33360.32.0e-13872.53NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G33360.29.5e-12071.28NAD(P)-binding Rossmann-fold superfamily protein [more]
AT1G09480.15.9e-2129.89NAD(P)-binding Rossmann-fold superfamily protein [more]
AT1G09490.11.3e-2029.23NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001509NAD-dependent epimerase/dehydratasePFAMPF01370Epimerasecoord: 3..220
e-value: 1.2E-27
score: 97.0
NoneNo IPR availableGENE3D3.40.50.720coord: 1..315
e-value: 5.1E-91
score: 307.1
NoneNo IPR availablePANTHERPTHR10366:SF624NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEINcoord: 3..313
NoneNo IPR availablePANTHERPTHR10366NAD DEPENDENT EPIMERASE/DEHYDRATASEcoord: 3..313
NoneNo IPR availableCDDcd05228AR_FR_like_1_SDR_ecoord: 3..312
e-value: 6.62851E-123
score: 353.899
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 1..312

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0000209.1PI0000209.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0003824 catalytic activity