PI0000048 (gene) Melon (PI 482460) v1

Overview
NamePI0000048
Typegene
OrganismCucumis metuliferus (Melon (PI 482460) v1)
Descriptionribulose bisphosphate carboxylase/oxygenase activase, chloroplastic
Locationchr10: 15740677 .. 15742973 (+)
RNA-Seq ExpressionPI0000048
SyntenyPI0000048
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTGGTTGTTGAGAAATTTTGAAACTCATGGTTAGGATATCATTTTTCCAATGGACTCAAAACCTCCATCCCTTTACTTCTATAAAGTCCCTCTTCACTCCTCACTCCTCTTTACCCTTCTCCAAATCTACCTTCCTCTGCCAAATACTTACACACTCCAAGTCCCCATGGCCGCCTCCGCTGCCTCTGTCGGAGTCGTTAACCATGCACCGGTAAGTTCTTCTCTATTACGCCACTTGAATTTAGATTTCTAATGCTCTAATACCATGTTAAAGTTTATTACTGTAAATTTCAGCTGAGCTTGAATGGCTCTGGATCAGCAACTTCGGTTCCGAGTTCAGTGTTCTTCGGGAACAGTTTGAAGAAAGTGGTGAACTCGAGGACTGTTAACGCGAAGGCTTCGTCAGGTAGCTTCAAGGTGGTGGCAGTGGAATCAATCCCAGACGAGAATCTCGAGAAGCAGACGAGCAAAGACAGATGGTCAGGACTTGGATATGATCAGTCAGATGACCAACAGGATATCACAAGAGGAAAGGGAATGGTGGATTCTCTGTTTCAAGCTCCCATGCAATCTGGAACTCATTATGCTGTTATGAGCTCTTATGAGTATCTTAGTGCAGGACTTAAAACGTAAGACACACAAAAAGAAAAGAAAAAAAAAAATCATAATTTGTTGGTTGTTTTTTGAGAGATGATATATCTTGTTTTTGTATGTGTTGTAGGTATGAAGGCATGGACAATTTATTGGATGGTTACTACATTGCTCCTGCTTTCATGGACAAATTGGTTGTTCACATCACCAAGAACTTCATGAGCCTCCCAAACATCAAGGTTTCTAACCCTTTACATTCATCTAAACTGAGTTCTGTGATACTTTTGAGTCAAAACCATTGTTTTTATAAACATGATGCTATAAGATCACTATCACTAATTTTGGATCAGATCTAAACAAATTGAAGAAGTAACTCATCTATCTTGTAAAGAGTGAAGAGTGAGATCTATCAATAAAAATCATTAAGGAAATTCAAAGTCAATATATGTAAATTTTGTGTGTGCAGGTTCCTCTGATTTTGGGTCTGTGGGGAGGCAAAGGACAAGGAAAATCCTTCCAATGTGAGCTTGTATTTGCCAAGATGGGAGTCAAGTGAGTCTGTCCATAATCCTACTTTTCTAAAACTCATACTTTATACAAAAATTTGGTTTGCTCTTCTAACACTTTGGCCGTTTGCACAGCCCAATCATGATGAGTGCAGGAGAATTGGAAAGCGGGAACGCAGGAGAGCCAGCCAAGTTGATCCGGCAACGGTACCGTGAGGCGGCTGACATAATCAAGAAGGGAAAAATGTCCTGCCTCTTCATCAACGATCTGGATGCAGGTGCTGGTCGTCTTGGTGGTACCACTCAATACACCGTCAACAACCAAATGGTTAACGCCACACTCATGAACATCGCCGATAACCCCACCAATGTCCAACTCCCTGGAATGTACAACAAGGAGGAGAACCCCAGAGTTCCAATCATCGTCACTGGTAACGACTTCTCCACTCTCTACGCTCCTCTCATCCGTGACGGTCGTATGGAGAAATTCTACTGGGCACCCACTAGGGAAGACCGTATCGGTGTCTGCAAAGGTATCTTCCGTACCGACAATGTCGCAGATGAGGACATCGTCAAGTTGGTCGACACCTTCCCAGGCCAATCCATCGGTCAGTTACTACTCATGTCTCATTACATGACATTGCATTACATTACATAACAATCCATCCGTCGCTAGAGACTTAACTCTCTTTTATGCCTGTTTTTGTTGGACAGATTTCTTCGGTGCTCTAAGGGCGAGAGTGTACGATGACGAAGTAAGGAAGTGGATCATTGAAATTGGAGTCGATAGTGTAGGGAAGAAGCTCGTGAACTCAAGAGATCCACCACCGAAGTTTGAGCAGCCATCGATGACACTTCAAAAGCTGTTGGAGTACGGTAGCATGTTGGTTCAAGAACAAGAGAATGTGAAGAGGGTCCAATTGGCTGAAACTTATTTGAATGAGGCTGCTCTTGGCAATGCCAATGAAGATGCAATTACTAGAGGAGCTTTCTAAATTAAAGCTTGTGTGTTGTGTTAAACTCATTTCATGACACTCATCATCAAAGTTGTGTCTTTGTGGGGAAAGGAAATGAGTTCTCTCATTATGTGAGATTTGTCTCATCTATATATTCTTCAGGCCTTTTTTTTGTTCTTTCTTCTTCTTTTGTGGAACCAAAATCACATATCAAAATAAAAGTCATTTCTTACTTTTGTTTCTC

mRNA sequence

GTGGTTGTTGAGAAATTTTGAAACTCATGGTTAGGATATCATTTTTCCAATGGACTCAAAACCTCCATCCCTTTACTTCTATAAAGTCCCTCTTCACTCCTCACTCCTCTTTACCCTTCTCCAAATCTACCTTCCTCTGCCAAATACTTACACACTCCAAGTCCCCATGGCCGCCTCCGCTGCCTCTGTCGGAGTCGTTAACCATGCACCGCTGAGCTTGAATGGCTCTGGATCAGCAACTTCGGTTCCGAGTTCAGTGTTCTTCGGGAACAGTTTGAAGAAAGTGGTGAACTCGAGGACTGTTAACGCGAAGGCTTCGTCAGGTAGCTTCAAGGTGGTGGCAGTGGAATCAATCCCAGACGAGAATCTCGAGAAGCAGACGAGCAAAGACAGATGGTCAGGACTTGGATATGATCAGTCAGATGACCAACAGGATATCACAAGAGGAAAGGGAATGGTGGATTCTCTGTTTCAAGCTCCCATGCAATCTGGAACTCATTATGCTGTTATGAGCTCTTATGAGTATCTTAGTGCAGGACTTAAAACGTATGAAGGCATGGACAATTTATTGGATGGTTACTACATTGCTCCTGCTTTCATGGACAAATTGGTTGTTCACATCACCAAGAACTTCATGAGCCTCCCAAACATCAAGGTTCCTCTGATTTTGGGTCTGTGGGGAGGCAAAGGACAAGGAAAATCCTTCCAATGTGAGCTTGTATTTGCCAAGATGGGAGTCAACCCAATCATGATGAGTGCAGGAGAATTGGAAAGCGGGAACGCAGGAGAGCCAGCCAAGTTGATCCGGCAACGGTACCGTGAGGCGGCTGACATAATCAAGAAGGGAAAAATGTCCTGCCTCTTCATCAACGATCTGGATGCAGGTGCTGGTCGTCTTGGTGGTACCACTCAATACACCGTCAACAACCAAATGGTTAACGCCACACTCATGAACATCGCCGATAACCCCACCAATGTCCAACTCCCTGGAATGTACAACAAGGAGGAGAACCCCAGAGTTCCAATCATCGTCACTGGTAACGACTTCTCCACTCTCTACGCTCCTCTCATCCGTGACGGTCGTATGGAGAAATTCTACTGGGCACCCACTAGGGAAGACCGTATCGGTGTCTGCAAAGGTATCTTCCGTACCGACAATGTCGCAGATGAGGACATCGTCAAGTTGGTCGACACCTTCCCAGGCCAATCCATCGATTTCTTCGGTGCTCTAAGGGCGAGAGTGTACGATGACGAAGTAAGGAAGTGGATCATTGAAATTGGAGTCGATAGTGTAGGGAAGAAGCTCGTGAACTCAAGAGATCCACCACCGAAGTTTGAGCAGCCATCGATGACACTTCAAAAGCTGTTGGAGTACGGTAGCATGTTGGTTCAAGAACAAGAGAATGTGAAGAGGGTCCAATTGGCTGAAACTTATTTGAATGAGGCTGCTCTTGGCAATGCCAATGAAGATGCAATTACTAGAGGAGCTTTCTAAATTAAAGCTTGTGTGTTGTGTTAAACTCATTTCATGACACTCATCATCAAAGTTGTGTCTTTGTGGGGAAAGGAAATGAGTTCTCTCATTATGTGAGATTTGTCTCATCTATATATTCTTCAGGCCTTTTTTTTGTTCTTTCTTCTTCTTTTGTGGAACCAAAATCACATATCAAAATAAAAGTCATTTCTTACTTTTGTTTCTC

Coding sequence (CDS)

ATGGACTCAAAACCTCCATCCCTTTACTTCTATAAAGTCCCTCTTCACTCCTCACTCCTCTTTACCCTTCTCCAAATCTACCTTCCTCTGCCAAATACTTACACACTCCAAGTCCCCATGGCCGCCTCCGCTGCCTCTGTCGGAGTCGTTAACCATGCACCGCTGAGCTTGAATGGCTCTGGATCAGCAACTTCGGTTCCGAGTTCAGTGTTCTTCGGGAACAGTTTGAAGAAAGTGGTGAACTCGAGGACTGTTAACGCGAAGGCTTCGTCAGGTAGCTTCAAGGTGGTGGCAGTGGAATCAATCCCAGACGAGAATCTCGAGAAGCAGACGAGCAAAGACAGATGGTCAGGACTTGGATATGATCAGTCAGATGACCAACAGGATATCACAAGAGGAAAGGGAATGGTGGATTCTCTGTTTCAAGCTCCCATGCAATCTGGAACTCATTATGCTGTTATGAGCTCTTATGAGTATCTTAGTGCAGGACTTAAAACGTATGAAGGCATGGACAATTTATTGGATGGTTACTACATTGCTCCTGCTTTCATGGACAAATTGGTTGTTCACATCACCAAGAACTTCATGAGCCTCCCAAACATCAAGGTTCCTCTGATTTTGGGTCTGTGGGGAGGCAAAGGACAAGGAAAATCCTTCCAATGTGAGCTTGTATTTGCCAAGATGGGAGTCAACCCAATCATGATGAGTGCAGGAGAATTGGAAAGCGGGAACGCAGGAGAGCCAGCCAAGTTGATCCGGCAACGGTACCGTGAGGCGGCTGACATAATCAAGAAGGGAAAAATGTCCTGCCTCTTCATCAACGATCTGGATGCAGGTGCTGGTCGTCTTGGTGGTACCACTCAATACACCGTCAACAACCAAATGGTTAACGCCACACTCATGAACATCGCCGATAACCCCACCAATGTCCAACTCCCTGGAATGTACAACAAGGAGGAGAACCCCAGAGTTCCAATCATCGTCACTGGTAACGACTTCTCCACTCTCTACGCTCCTCTCATCCGTGACGGTCGTATGGAGAAATTCTACTGGGCACCCACTAGGGAAGACCGTATCGGTGTCTGCAAAGGTATCTTCCGTACCGACAATGTCGCAGATGAGGACATCGTCAAGTTGGTCGACACCTTCCCAGGCCAATCCATCGATTTCTTCGGTGCTCTAAGGGCGAGAGTGTACGATGACGAAGTAAGGAAGTGGATCATTGAAATTGGAGTCGATAGTGTAGGGAAGAAGCTCGTGAACTCAAGAGATCCACCACCGAAGTTTGAGCAGCCATCGATGACACTTCAAAAGCTGTTGGAGTACGGTAGCATGTTGGTTCAAGAACAAGAGAATGTGAAGAGGGTCCAATTGGCTGAAACTTATTTGAATGAGGCTGCTCTTGGCAATGCCAATGAAGATGCAATTACTAGAGGAGCTTTCTAA

Protein sequence

MDSKPPSLYFYKVPLHSSLLFTLLQIYLPLPNTYTLQVPMAASAASVGVVNHAPLSLNGSGSATSVPSSVFFGNSLKKVVNSRTVNAKASSGSFKVVAVESIPDENLEKQTSKDRWSGLGYDQSDDQQDITRGKGMVDSLFQAPMQSGTHYAVMSSYEYLSAGLKTYEGMDNLLDGYYIAPAFMDKLVVHITKNFMSLPNIKVPLILGLWGGKGQGKSFQCELVFAKMGVNPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAGAGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAPLIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARVYDDEVRKWIIEIGVDSVGKKLVNSRDPPPKFEQPSMTLQKLLEYGSMLVQEQENVKRVQLAETYLNEAALGNANEDAITRGAF
Homology
BLAST of PI0000048 vs. ExPASy Swiss-Prot
Match: Q7X9A0 (Ribulose bisphosphate carboxylase/oxygenase activase 1, chloroplastic OS=Larrea tridentata OX=66636 GN=RCA1 PE=1 SV=1)

HSP 1 Score: 726.1 bits (1873), Expect = 2.6e-208
Identity = 364/443 (82.17%), Postives = 399/443 (90.07%), Query Frame = 0

Query: 40  MAASAASVGVVNHAPLSLNGSGS-ATSVPSSVFFGNSLKKVVNSRTVNAKASSGSFKVVA 99
           MAA+ ++VG VN APLSLNGSG+ A+ VPS+ FFG+SLKK   S     KASSG+FK+VA
Sbjct: 1   MAAAYSTVGAVNRAPLSLNGSGARASLVPSTAFFGSSLKK---SAAKFPKASSGNFKIVA 60

Query: 100 VESIPDENLEKQTSKDRWSGLGYDQSDDQQDITRGKGMVDSLFQAPMQSGTHYAVMSSYE 159
            E   D+    QT KD+W GL YD SDDQQDITRGKGMVD+LFQAPMQSGTHYAVMSSY+
Sbjct: 61  QEISEDQ----QTDKDKWKGLAYDISDDQQDITRGKGMVDTLFQAPMQSGTHYAVMSSYD 120

Query: 160 YLSAGLKTYEGMDNLLDGYYIAPAFMDKLVVHITKNFMSLPNIKVPLILGLWGGKGQGKS 219
           Y+S GL+ Y  +DN +DG+YIAPAFMDKLVVHITKNF+SLPNIK+PLILG+WGGKGQGKS
Sbjct: 121 YISQGLRQY-NLDNNMDGFYIAPAFMDKLVVHITKNFLSLPNIKIPLILGIWGGKGQGKS 180

Query: 220 FQCELVFAKMGVNPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDA 279
           FQCELVFAKMG+NPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKM CLFINDLDA
Sbjct: 181 FQCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCCLFINDLDA 240

Query: 280 GAGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYA 339
           GAGR+GGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYA
Sbjct: 241 GAGRMGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYA 300

Query: 340 PLIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARV 399
           PLIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVAD+DIVKLVDTFPGQSIDFFGALRARV
Sbjct: 301 PLIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVADDDIVKLVDTFPGQSIDFFGALRARV 360

Query: 400 YDDEVRKWIIEIGVDSVGKKLVNSRDPPPKFEQPSMTLQKLLEYGSMLVQEQENVKRVQL 459
           Y DEVRKW+ E+GVD++GKKLVNS++ PP FEQP MT+ KLL YG MLVQEQENVKRVQL
Sbjct: 361 YHDEVRKWVSEVGVDTIGKKLVNSKEGPPSFEQPKMTIDKLLGYGGMLVQEQENVKRVQL 420

Query: 460 AETYLNEAALGNANEDAITRGAF 482
           A+ Y++EAALG+AN DAI RG F
Sbjct: 421 ADKYMSEAALGDANNDAIKRGTF 435

BLAST of PI0000048 vs. ExPASy Swiss-Prot
Match: Q7X999 (Ribulose bisphosphate carboxylase/oxygenase activase 2, chloroplastic OS=Larrea tridentata OX=66636 GN=RCA2 PE=2 SV=1)

HSP 1 Score: 724.2 bits (1868), Expect = 9.9e-208
Identity = 361/443 (81.49%), Postives = 401/443 (90.52%), Query Frame = 0

Query: 40  MAASAASVGVVNHAPLSLNGSGS-ATSVPSSVFFGNSLKKVVNSRTVNAKASSGSFKVVA 99
           MAA+ ++VG VN APLSLNGSG+ A+ VPS+ FFG+SLKK   S     KASSG+FK+VA
Sbjct: 1   MAAAYSTVGAVNRAPLSLNGSGARASLVPSTAFFGSSLKK---SAAKFPKASSGNFKIVA 60

Query: 100 VESIPDENLEKQTSKDRWSGLGYDQSDDQQDITRGKGMVDSLFQAPMQSGTHYAVMSSYE 159
            E   D+    QT KD+W GL YD SDDQQDITRGKGMVD+LFQAPMQSGTHYAVMSSY+
Sbjct: 61  QEISEDQ----QTDKDKWKGLAYDISDDQQDITRGKGMVDTLFQAPMQSGTHYAVMSSYD 120

Query: 160 YLSAGLKTYEGMDNLLDGYYIAPAFMDKLVVHITKNFMSLPNIKVPLILGLWGGKGQGKS 219
           Y+S GL+ Y  +DN +DG+YIAPAFMDKLVVHITKNF+SLPNIK+PLILG+WGGKGQGKS
Sbjct: 121 YISQGLRQY-NLDNNMDGFYIAPAFMDKLVVHITKNFLSLPNIKIPLILGIWGGKGQGKS 180

Query: 220 FQCELVFAKMGVNPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDA 279
           FQCELVFAKMG+NPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKM CLFINDLDA
Sbjct: 181 FQCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMCCLFINDLDA 240

Query: 280 GAGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYA 339
           GAGR+GGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYA
Sbjct: 241 GAGRMGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYA 300

Query: 340 PLIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARV 399
           PLIRDGRMEKFYWAPTREDRIGVCKGIFRTDNV +EDIVK+VD FPGQSIDFFGALRARV
Sbjct: 301 PLIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVPEEDIVKVVDQFPGQSIDFFGALRARV 360

Query: 400 YDDEVRKWIIEIGVDSVGKKLVNSRDPPPKFEQPSMTLQKLLEYGSMLVQEQENVKRVQL 459
           YDDEVRKW+ E+GVD++GKKLVNS++ PP FEQP MT+ KLL+YG+MLV+EQENVKRVQL
Sbjct: 361 YDDEVRKWVSEVGVDTIGKKLVNSKEGPPTFEQPKMTIDKLLQYGNMLVEEQENVKRVQL 420

Query: 460 AETYLNEAALGNANEDAITRGAF 482
           A+ Y++EAALG+AN+DAI RG F
Sbjct: 421 ADKYMSEAALGDANQDAIKRGTF 435

BLAST of PI0000048 vs. ExPASy Swiss-Prot
Match: O98997 (Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Vigna radiata var. radiata OX=3916 GN=RCA PE=2 SV=2)

HSP 1 Score: 720.7 bits (1859), Expect = 1.1e-206
Identity = 358/442 (81.00%), Postives = 394/442 (89.14%), Query Frame = 0

Query: 40  MAASAASVGVVNHAPLSLNGSGSATSVPSSVFFGNSLKKVVNSRTVNAKASSGSFKVVAV 99
           MAAS ++VG VN A L+LNGSG+  S P+S FFG SLKK V SR  N+K ++GSFK+VA 
Sbjct: 1   MAASVSTVGAVNRAILNLNGSGAGASAPTSAFFGTSLKKAVASRVPNSKVTNGSFKIVAA 60

Query: 100 ESIPDENLEKQTSKDRWSGLGYDQSDDQQDITRGKGMVDSLFQAPMQSGTHYAVMSSYEY 159
           E   +E+  +QT+KDRW GL YD SDDQQDITRGKGMVD LFQAPM +GTHYAVMSSYEY
Sbjct: 61  EKEIEES--QQTNKDRWKGLAYDISDDQQDITRGKGMVDPLFQAPMDAGTHYAVMSSYEY 120

Query: 160 LSAGLKTYEGMDNLLDGYYIAPAFMDKLVVHITKNFMSLPNIKVPLILGLWGGKGQGKSF 219
           LS GL+    +DN+ DG+YIAPAF+DKLVVHITKNFM+LPNIKVPLILG+WGGKGQGKSF
Sbjct: 121 LSTGLRQ---LDNIKDGFYIAPAFLDKLVVHITKNFMTLPNIKVPLILGIWGGKGQGKSF 180

Query: 220 QCELVFAKMGVNPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG 279
           QCELVFAKMG+NPIMMSAGELESGNAGEPAKLIRQRYREAAD+I KGKM  LFINDLDAG
Sbjct: 181 QCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIAKGKMCALFINDLDAG 240

Query: 280 AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAP 339
           AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEEN RVPIIVTGNDFSTLYAP
Sbjct: 241 AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENARVPIIVTGNDFSTLYAP 300

Query: 340 LIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARVY 399
           LIRDGRMEKFYWAPTR+DR+GVCKGIFRTD V +EDI KLVDTFPGQSIDFFGALRARVY
Sbjct: 301 LIRDGRMEKFYWAPTRDDRVGVCKGIFRTDGVPEEDITKLVDTFPGQSIDFFGALRARVY 360

Query: 400 DDEVRKWIIEIGVDSVGKKLVNSRDPPPKFEQPSMTLQKLLEYGSMLVQEQENVKRVQLA 459
           DDEVRKWI  +GVD+ GKKLVNS++ PP F+QP M+L KLL+YG+MLVQEQENVKRVQLA
Sbjct: 361 DDEVRKWISGVGVDATGKKLVNSKEGPPTFDQPKMSLDKLLQYGNMLVQEQENVKRVQLA 420

Query: 460 ETYLNEAALGNANEDAITRGAF 482
           + YLNEAALGNANEDAI  G+F
Sbjct: 421 DKYLNEAALGNANEDAIKSGSF 437

BLAST of PI0000048 vs. ExPASy Swiss-Prot
Match: Q40281 (Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Malus domestica OX=3750 GN=RCA PE=2 SV=1)

HSP 1 Score: 711.8 bits (1836), Expect = 5.1e-204
Identity = 357/442 (80.77%), Postives = 394/442 (89.14%), Query Frame = 0

Query: 40  MAASAASVGVVNHAPLSLNGSGSATSVPSSVFFGNSLKKVVNSRTVNAKASSGSFKVVAV 99
           MA + +++G VN AP +LNGS S+ SVPSS F G+SLKK VNSR  N+K SSGS ++VA 
Sbjct: 1   MATAVSTIGSVNRAPPNLNGSSSSASVPSSTFLGSSLKK-VNSRFTNSKVSSGSLRIVA- 60

Query: 100 ESIPDENLEKQTSKDRWSGLGYDQSDDQQDITRGKGMVDSLFQAPMQSGTHYAVMSSYEY 159
            S+ +   +KQT KDRW GL +D SDDQQDITRGKG VDSLFQAP  SGTH+A+MSSYEY
Sbjct: 61  -SVDE---DKQTDKDRWKGLAFDTSDDQQDITRGKGKVDSLFQAPQGSGTHFAIMSSYEY 120

Query: 160 LSAGLKTYEGMDNLLDGYYIAPAFMDKLVVHITKNFMSLPNIKVPLILGLWGGKGQGKSF 219
           +S GL+ Y   DN +DGYYIAPAFMDKLVVHITKNFM+LPN+KVPLILG+WGGKGQGKSF
Sbjct: 121 ISTGLRQY-NFDNNMDGYYIAPAFMDKLVVHITKNFMTLPNMKVPLILGIWGGKGQGKSF 180

Query: 220 QCELVFAKMGVNPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG 279
           QCELVFAKM ++PIMMSAGELESGNAGEPAKLIRQRYREAADII+KGKM  LFINDLDAG
Sbjct: 181 QCELVFAKMRISPIMMSAGELESGNAGEPAKLIRQRYREAADIIRKGKMCALFINDLDAG 240

Query: 280 AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAP 339
           AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAP
Sbjct: 241 AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAP 300

Query: 340 LIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARVY 399
           LIRDGRMEKFYWAPTREDRIGVC GIFR+DNVA EDIVKLVDTFPGQSIDFFGALRARVY
Sbjct: 301 LIRDGRMEKFYWAPTREDRIGVCIGIFRSDNVAKEDIVKLVDTFPGQSIDFFGALRARVY 360

Query: 400 DDEVRKWIIEIGVDSVGKKLVNSRDPPPKFEQPSMTLQKLLEYGSMLVQEQENVKRVQLA 459
           DDEVRKWI  +GVDS+GKKLVNS++ PP FEQP MT++KLLEYG+MLVQEQENVKRVQLA
Sbjct: 361 DDEVRKWITGVGVDSIGKKLVNSKEGPPTFEQPKMTIEKLLEYGNMLVQEQENVKRVQLA 420

Query: 460 ETYLNEAALGNANEDAITRGAF 482
           + YL+EAALG+AN DA+  G F
Sbjct: 421 DKYLSEAALGDANSDAMNTGTF 435

BLAST of PI0000048 vs. ExPASy Swiss-Prot
Match: O64981 (Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Phaseolus vulgaris OX=3885 GN=RCA1 PE=2 SV=1)

HSP 1 Score: 709.9 bits (1831), Expect = 1.9e-203
Identity = 356/442 (80.54%), Postives = 391/442 (88.46%), Query Frame = 0

Query: 40  MAASAASVGVVNHAPLSLNGSGSATSVPSSVFFGNSLKKVVNSRTVNAKASSGSFKVVAV 99
           MAAS ++VG VN   L+LNGSG   S PSS FFG SLKKV++SR  N+K +SGSFK+VA 
Sbjct: 1   MAASLSTVGAVNRTLLNLNGSGGGASGPSSAFFGTSLKKVISSRVPNSKLTSGSFKIVAA 60

Query: 100 ESIPDENLEKQTSKDRWSGLGYDQSDDQQDITRGKGMVDSLFQAPMQSGTHYAVMSSYEY 159
           +   +E   +QT  DRW GL YD SDDQQDITRGKG+VDSLFQAPM +GTHYAV+SS++Y
Sbjct: 61  DKEIEET--QQTEGDRWRGLAYDVSDDQQDITRGKGLVDSLFQAPMDAGTHYAVISSHKY 120

Query: 160 LSAGLKTYEGMDNLLDGYYIAPAFMDKLVVHITKNFMSLPNIKVPLILGLWGGKGQGKSF 219
           LSAGL+ Y   DN+ DG+YIAPAF+DKLVVHI KNFM+LPNIKVPLILG+WGGKGQGKSF
Sbjct: 121 LSAGLRQY-NFDNIKDGFYIAPAFLDKLVVHIAKNFMTLPNIKVPLILGVWGGKGQGKSF 180

Query: 220 QCELVFAKMGVNPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG 279
           QCELVFAKMG+NPIMMSAGELESGNAGEPAKLIRQRYREA+D+IKKGKM  LFINDLDAG
Sbjct: 181 QCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREASDLIKKGKMCVLFINDLDAG 240

Query: 280 AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAP 339
           AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKE+N RVPIIVTGNDFSTLYAP
Sbjct: 241 AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEDNARVPIIVTGNDFSTLYAP 300

Query: 340 LIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARVY 399
           LIRDGRMEKFYWAPTREDRIGVCKGIFRTD V ++DIV+LVD  PGQSIDFFGALRARVY
Sbjct: 301 LIRDGRMEKFYWAPTREDRIGVCKGIFRTDGVPEKDIVELVDKHPGQSIDFFGALRARVY 360

Query: 400 DDEVRKWIIEIGVDSVGKKLVNSRDPPPKFEQPSMTLQKLLEYGSMLVQEQENVKRVQLA 459
           DDEVRKWI  +GVDSVGKKLVNS++ PP F+QP MTL KLL Y SMLVQEQENVKRVQLA
Sbjct: 361 DDEVRKWISGVGVDSVGKKLVNSKEGPPTFDQPKMTLDKLLLYASMLVQEQENVKRVQLA 420

Query: 460 ETYLNEAALGNANEDAITRGAF 482
           + YLNEAALGNANEDAI  G+F
Sbjct: 421 DQYLNEAALGNANEDAIKSGSF 439

BLAST of PI0000048 vs. ExPASy TrEMBL
Match: A0A5A7U526 (Ribulose bisphosphate carboxylase/oxygenase activase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold293G00550 PE=4 SV=1)

HSP 1 Score: 855.9 bits (2210), Expect = 8.1e-245
Identity = 432/467 (92.51%), Postives = 449/467 (96.15%), Query Frame = 0

Query: 16  HSSLLFTLLQIYLPLPNTYT-LQVPMAASAASVGVVNHAPLSLNGSGSATSVPSSVFFGN 75
           HSSL F+  +   PLPNTY+ LQVPMAASAASVGVVNHAPLSLNGSGSATSVPSSVFFG 
Sbjct: 27  HSSLSFS--KSTFPLPNTYSLLQVPMAASAASVGVVNHAPLSLNGSGSATSVPSSVFFG- 86

Query: 76  SLKKVVNSRTVNAKASSGSFKVVAVESIPDENLEKQTSKDRWSGLGYDQSDDQQDITRGK 135
             KKVV SR V  KASSGSFKVVAVESIPDENLEKQT+KDRW+GLGYD SDDQQDITRGK
Sbjct: 87  --KKVVTSRAVTPKASSGSFKVVAVESIPDENLEKQTNKDRWAGLGYDISDDQQDITRGK 146

Query: 136 GMVDSLFQAPMQSGTHYAVMSSYEYLSAGLKTYEGMDNLLDGYYIAPAFMDKLVVHITKN 195
           GMVDSLFQAPMQSGTHYAVMSSYEYLS GLKTYEGMDN+LDGYYIAPAFMDKLVVHITKN
Sbjct: 147 GMVDSLFQAPMQSGTHYAVMSSYEYLSTGLKTYEGMDNILDGYYIAPAFMDKLVVHITKN 206

Query: 196 FMSLPNIKVPLILGLWGGKGQGKSFQCELVFAKMGVNPIMMSAGELESGNAGEPAKLIRQ 255
           +M+LPNIKVPLILGLWGGKGQGKSFQCELVFAKMG+NPIMMSAGELESGNAGEPAKLIRQ
Sbjct: 207 YMTLPNIKVPLILGLWGGKGQGKSFQCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQ 266

Query: 256 RYREAADIIKKGKMSCLFINDLDAGAGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPG 315
           RYREAADIIKKGKMSCLFINDLDAGAGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPG
Sbjct: 267 RYREAADIIKKGKMSCLFINDLDAGAGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPG 326

Query: 316 MYNKEENPRVPIIVTGNDFSTLYAPLIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVADE 375
           MYNKEENPRVPIIVTGNDFSTLYAPLIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVADE
Sbjct: 327 MYNKEENPRVPIIVTGNDFSTLYAPLIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVADE 386

Query: 376 DIVKLVDTFPGQSIDFFGALRARVYDDEVRKWIIEIGVDSVGKKLVNSRDPPPKFEQPSM 435
           DIVKLVDTFPGQSIDFFGALRARVYDDEVRKW++ +GV ++GKKLVNS++PPPKFEQP+M
Sbjct: 387 DIVKLVDTFPGQSIDFFGALRARVYDDEVRKWVVAVGVQAIGKKLVNSKEPPPKFEQPAM 446

Query: 436 TLQKLLEYGSMLVQEQENVKRVQLAETYLNEAALGNANEDAITRGAF 482
           TLQKLLEYGSMLVQEQENVKRVQLAETYLNEAALGNANEDAITRGAF
Sbjct: 447 TLQKLLEYGSMLVQEQENVKRVQLAETYLNEAALGNANEDAITRGAF 488

BLAST of PI0000048 vs. ExPASy TrEMBL
Match: A0A1S3CF76 (ribulose bisphosphate carboxylase/oxygenase activase 2, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103500234 PE=4 SV=1)

HSP 1 Score: 836.6 bits (2160), Expect = 5.1e-239
Identity = 417/442 (94.34%), Postives = 431/442 (97.51%), Query Frame = 0

Query: 40  MAASAASVGVVNHAPLSLNGSGSATSVPSSVFFGNSLKKVVNSRTVNAKASSGSFKVVAV 99
           MAASAASVGVVNHAPLSLNGSGSATSVPSSVFFG   KKVV SR V  KASSGSFKVVAV
Sbjct: 1   MAASAASVGVVNHAPLSLNGSGSATSVPSSVFFG---KKVVTSRAVTPKASSGSFKVVAV 60

Query: 100 ESIPDENLEKQTSKDRWSGLGYDQSDDQQDITRGKGMVDSLFQAPMQSGTHYAVMSSYEY 159
           ESIPDENLEKQT+KDRW+GLGYD SDDQQDITRGKGMVDSLFQAPMQSGTHYAVMSSYEY
Sbjct: 61  ESIPDENLEKQTNKDRWAGLGYDISDDQQDITRGKGMVDSLFQAPMQSGTHYAVMSSYEY 120

Query: 160 LSAGLKTYEGMDNLLDGYYIAPAFMDKLVVHITKNFMSLPNIKVPLILGLWGGKGQGKSF 219
           LS GLKTYEGMDN+LDGYYIAPAFMDKLVVHITKN+M+LPNIKVPLILGLWGGKGQGKSF
Sbjct: 121 LSTGLKTYEGMDNILDGYYIAPAFMDKLVVHITKNYMTLPNIKVPLILGLWGGKGQGKSF 180

Query: 220 QCELVFAKMGVNPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG 279
           QCELVFAKMG+NPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG
Sbjct: 181 QCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG 240

Query: 280 AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAP 339
           AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAP
Sbjct: 241 AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAP 300

Query: 340 LIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARVY 399
           LIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARVY
Sbjct: 301 LIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARVY 360

Query: 400 DDEVRKWIIEIGVDSVGKKLVNSRDPPPKFEQPSMTLQKLLEYGSMLVQEQENVKRVQLA 459
           DDEVRKW++ +GV ++GKKLVNS++PPPKFEQP+MTLQKLLEYGSMLVQEQENVKRVQLA
Sbjct: 361 DDEVRKWVVAVGVQAIGKKLVNSKEPPPKFEQPAMTLQKLLEYGSMLVQEQENVKRVQLA 420

Query: 460 ETYLNEAALGNANEDAITRGAF 482
           ETYLNEAALGNANEDAITRGAF
Sbjct: 421 ETYLNEAALGNANEDAITRGAF 439

BLAST of PI0000048 vs. ExPASy TrEMBL
Match: A0A6J1K8Q9 (ribulose bisphosphate carboxylase/oxygenase activase 2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111491148 PE=4 SV=1)

HSP 1 Score: 808.5 bits (2087), Expect = 1.5e-230
Identity = 400/442 (90.50%), Postives = 423/442 (95.70%), Query Frame = 0

Query: 40  MAASAASVGVVNHAPLSLNGSGSATSVPSSVFFGNSLKKVVNSRTVNAKASSGSFKVVAV 99
           MAASAASVGV+NHAPLSLNGS SATSVPSSVFFG+SLKKVVNSR  + KAS GSFKVVA 
Sbjct: 1   MAASAASVGVINHAPLSLNGSSSATSVPSSVFFGSSLKKVVNSRIASPKASPGSFKVVAS 60

Query: 100 ESIPDENLEKQTSKDRWSGLGYDQSDDQQDITRGKGMVDSLFQAPMQSGTHYAVMSSYEY 159
           ++  DE+LEKQTSKDRW GL YD SDDQQDITRGKGMVD+LFQAPMQSGTH A+MSSYEY
Sbjct: 61  DAPADEDLEKQTSKDRWRGLAYDISDDQQDITRGKGMVDTLFQAPMQSGTHNAIMSSYEY 120

Query: 160 LSAGLKTYEGMDNLLDGYYIAPAFMDKLVVHITKNFMSLPNIKVPLILGLWGGKGQGKSF 219
           LS GLKTYEG+DNLLDG+YIAPAFMDKLVVHITKNFMSLPNIKVPLILG+WGGKGQGKSF
Sbjct: 121 LSTGLKTYEGLDNLLDGFYIAPAFMDKLVVHITKNFMSLPNIKVPLILGIWGGKGQGKSF 180

Query: 220 QCELVFAKMGVNPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG 279
           QCELVFAKMG+ PIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG
Sbjct: 181 QCELVFAKMGITPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG 240

Query: 280 AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAP 339
           AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKE+NPRVPIIVTGNDFSTLYAP
Sbjct: 241 AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEDNPRVPIIVTGNDFSTLYAP 300

Query: 340 LIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARVY 399
           LIRDGRMEKFYWAPTREDRIGVCKGIFRTDNV DED+V+LVDTFPGQSIDFFGALRARVY
Sbjct: 301 LIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVPDEDVVRLVDTFPGQSIDFFGALRARVY 360

Query: 400 DDEVRKWIIEIGVDSVGKKLVNSRDPPPKFEQPSMTLQKLLEYGSMLVQEQENVKRVQLA 459
           DDEVRKWII +GVD+VGKKLVNS+DPPPKFEQP M+++KLLEYG+MLVQEQENVKRVQLA
Sbjct: 361 DDEVRKWIIGVGVDTVGKKLVNSKDPPPKFEQPKMSVEKLLEYGNMLVQEQENVKRVQLA 420

Query: 460 ETYLNEAALGNANEDAITRGAF 482
           + YLNEAALGNANEDAI RGAF
Sbjct: 421 DKYLNEAALGNANEDAINRGAF 442

BLAST of PI0000048 vs. ExPASy TrEMBL
Match: A0A0A0KLW9 (Beta-form rubisco activase OS=Cucumis sativus OX=3659 GN=Csa_5G182730 PE=4 SV=1)

HSP 1 Score: 808.5 bits (2087), Expect = 1.5e-230
Identity = 404/443 (91.20%), Postives = 423/443 (95.49%), Query Frame = 0

Query: 40  MAASAASVGVVNHAPLSLNGSGSATSVPSSVFFGNSLKKVVNSRTVNAKASSGSFKVVAV 99
           MAASAASVGVVNHAPLSL GSGS TSVPSSVFFGNSLKKVVNSR VN K SSGSFKV+AV
Sbjct: 1   MAASAASVGVVNHAPLSLKGSGSTTSVPSSVFFGNSLKKVVNSRVVNPKPSSGSFKVMAV 60

Query: 100 ESIPDENLEKQTSK-DRWSGLGYDQSDDQQDITRGKGMVDSLFQAPMQSGTHYAVMSSYE 159
           ES  DENLEK+T K DRW+GLG D SDDQQDITRGKGMVDS+FQAPMQ+GTHYAVMSSYE
Sbjct: 61  ESTSDENLEKKTKKIDRWAGLGTDISDDQQDITRGKGMVDSVFQAPMQAGTHYAVMSSYE 120

Query: 160 YLSAGLKTYEGMDNLLDGYYIAPAFMDKLVVHITKNFMSLPNIKVPLILGLWGGKGQGKS 219
           YLS G K+Y+GMDN+L G YIAPAFMDKLVVHITKNF+SLPNIKVPLILGLWGGKGQGKS
Sbjct: 121 YLSQGRKSYDGMDNVLGGLYIAPAFMDKLVVHITKNFLSLPNIKVPLILGLWGGKGQGKS 180

Query: 220 FQCELVFAKMGVNPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDA 279
           FQCELVFAKMG+ PIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDA
Sbjct: 181 FQCELVFAKMGITPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDA 240

Query: 280 GAGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYA 339
           GAGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYA
Sbjct: 241 GAGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYA 300

Query: 340 PLIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARV 399
           PLIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVAD+DIVKLVDTFPGQSIDFFGALRARV
Sbjct: 301 PLIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVADDDIVKLVDTFPGQSIDFFGALRARV 360

Query: 400 YDDEVRKWIIEIGVDSVGKKLVNSRDPPPKFEQPSMTLQKLLEYGSMLVQEQENVKRVQL 459
           YDDEVRKW+  +GV S+GKKLVNS++PPPKFEQP+M+L+KLLEYG MLVQEQENVKRVQL
Sbjct: 361 YDDEVRKWVTGVGVQSIGKKLVNSKEPPPKFEQPTMSLEKLLEYGGMLVQEQENVKRVQL 420

Query: 460 AETYLNEAALGNANEDAITRGAF 482
           AETYLNEAALGNANEDAITRGAF
Sbjct: 421 AETYLNEAALGNANEDAITRGAF 443

BLAST of PI0000048 vs. ExPASy TrEMBL
Match: A0A6J1FN78 (ribulose bisphosphate carboxylase/oxygenase activase 2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111446980 PE=4 SV=1)

HSP 1 Score: 806.6 bits (2082), Expect = 5.6e-230
Identity = 398/442 (90.05%), Postives = 423/442 (95.70%), Query Frame = 0

Query: 40  MAASAASVGVVNHAPLSLNGSGSATSVPSSVFFGNSLKKVVNSRTVNAKASSGSFKVVAV 99
           MAASAASVGV+NHAPLSLNGS SATSVPSSVFFG+SLKKVVNSR  + KAS GSFKVVA 
Sbjct: 1   MAASAASVGVINHAPLSLNGSSSATSVPSSVFFGSSLKKVVNSRIASPKASPGSFKVVAS 60

Query: 100 ESIPDENLEKQTSKDRWSGLGYDQSDDQQDITRGKGMVDSLFQAPMQSGTHYAVMSSYEY 159
           ++  DE+L+KQTSKDRW GL YD SDDQQDITRGKGMVD+LFQAPMQSGTH A+MSSYEY
Sbjct: 61  DAPADEDLDKQTSKDRWRGLAYDISDDQQDITRGKGMVDTLFQAPMQSGTHNAIMSSYEY 120

Query: 160 LSAGLKTYEGMDNLLDGYYIAPAFMDKLVVHITKNFMSLPNIKVPLILGLWGGKGQGKSF 219
           LS GLKTYEG+DNLLDG+YIAPAFMDKLVVHITKNFMSLPNIKVPLILG+WGGKGQGKSF
Sbjct: 121 LSTGLKTYEGLDNLLDGFYIAPAFMDKLVVHITKNFMSLPNIKVPLILGIWGGKGQGKSF 180

Query: 220 QCELVFAKMGVNPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG 279
           QCELVFAKMG+ PIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG
Sbjct: 181 QCELVFAKMGITPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG 240

Query: 280 AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAP 339
           AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKE+NPRVPIIVTGNDFSTLYAP
Sbjct: 241 AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEDNPRVPIIVTGNDFSTLYAP 300

Query: 340 LIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARVY 399
           LIRDGRMEKFYWAPTREDRIGVCKGIFRTDNV DED+V+LVDTFPGQSIDFFGALRARVY
Sbjct: 301 LIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVPDEDVVRLVDTFPGQSIDFFGALRARVY 360

Query: 400 DDEVRKWIIEIGVDSVGKKLVNSRDPPPKFEQPSMTLQKLLEYGSMLVQEQENVKRVQLA 459
           DDEVRKWI+ +GVD+VGKKLVNS+DPPPKFEQP M+++KLLEYG+MLVQEQENVKRVQLA
Sbjct: 361 DDEVRKWIVGVGVDTVGKKLVNSKDPPPKFEQPKMSVEKLLEYGNMLVQEQENVKRVQLA 420

Query: 460 ETYLNEAALGNANEDAITRGAF 482
           + YLNEAALGNANEDAI RGAF
Sbjct: 421 DKYLNEAALGNANEDAIKRGAF 442

BLAST of PI0000048 vs. NCBI nr
Match: KAA0050344.1 (ribulose bisphosphate carboxylase/oxygenase activase 2 [Cucumis melo var. makuwa] >TYK03558.1 ribulose bisphosphate carboxylase/oxygenase activase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 855.9 bits (2210), Expect = 1.7e-244
Identity = 432/467 (92.51%), Postives = 449/467 (96.15%), Query Frame = 0

Query: 16  HSSLLFTLLQIYLPLPNTYT-LQVPMAASAASVGVVNHAPLSLNGSGSATSVPSSVFFGN 75
           HSSL F+  +   PLPNTY+ LQVPMAASAASVGVVNHAPLSLNGSGSATSVPSSVFFG 
Sbjct: 27  HSSLSFS--KSTFPLPNTYSLLQVPMAASAASVGVVNHAPLSLNGSGSATSVPSSVFFG- 86

Query: 76  SLKKVVNSRTVNAKASSGSFKVVAVESIPDENLEKQTSKDRWSGLGYDQSDDQQDITRGK 135
             KKVV SR V  KASSGSFKVVAVESIPDENLEKQT+KDRW+GLGYD SDDQQDITRGK
Sbjct: 87  --KKVVTSRAVTPKASSGSFKVVAVESIPDENLEKQTNKDRWAGLGYDISDDQQDITRGK 146

Query: 136 GMVDSLFQAPMQSGTHYAVMSSYEYLSAGLKTYEGMDNLLDGYYIAPAFMDKLVVHITKN 195
           GMVDSLFQAPMQSGTHYAVMSSYEYLS GLKTYEGMDN+LDGYYIAPAFMDKLVVHITKN
Sbjct: 147 GMVDSLFQAPMQSGTHYAVMSSYEYLSTGLKTYEGMDNILDGYYIAPAFMDKLVVHITKN 206

Query: 196 FMSLPNIKVPLILGLWGGKGQGKSFQCELVFAKMGVNPIMMSAGELESGNAGEPAKLIRQ 255
           +M+LPNIKVPLILGLWGGKGQGKSFQCELVFAKMG+NPIMMSAGELESGNAGEPAKLIRQ
Sbjct: 207 YMTLPNIKVPLILGLWGGKGQGKSFQCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQ 266

Query: 256 RYREAADIIKKGKMSCLFINDLDAGAGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPG 315
           RYREAADIIKKGKMSCLFINDLDAGAGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPG
Sbjct: 267 RYREAADIIKKGKMSCLFINDLDAGAGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPG 326

Query: 316 MYNKEENPRVPIIVTGNDFSTLYAPLIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVADE 375
           MYNKEENPRVPIIVTGNDFSTLYAPLIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVADE
Sbjct: 327 MYNKEENPRVPIIVTGNDFSTLYAPLIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVADE 386

Query: 376 DIVKLVDTFPGQSIDFFGALRARVYDDEVRKWIIEIGVDSVGKKLVNSRDPPPKFEQPSM 435
           DIVKLVDTFPGQSIDFFGALRARVYDDEVRKW++ +GV ++GKKLVNS++PPPKFEQP+M
Sbjct: 387 DIVKLVDTFPGQSIDFFGALRARVYDDEVRKWVVAVGVQAIGKKLVNSKEPPPKFEQPAM 446

Query: 436 TLQKLLEYGSMLVQEQENVKRVQLAETYLNEAALGNANEDAITRGAF 482
           TLQKLLEYGSMLVQEQENVKRVQLAETYLNEAALGNANEDAITRGAF
Sbjct: 447 TLQKLLEYGSMLVQEQENVKRVQLAETYLNEAALGNANEDAITRGAF 488

BLAST of PI0000048 vs. NCBI nr
Match: XP_038890850.1 (ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 845.1 bits (2182), Expect = 2.9e-241
Identity = 419/442 (94.80%), Postives = 433/442 (97.96%), Query Frame = 0

Query: 40  MAASAASVGVVNHAPLSLNGSGSATSVPSSVFFGNSLKKVVNSRTVNAKASSGSFKVVAV 99
           MAASAASVGVVNHAPLSLNGSGSATSVPSSVFFGNSLKKVVNSR VN KASSGSFKVVA 
Sbjct: 1   MAASAASVGVVNHAPLSLNGSGSATSVPSSVFFGNSLKKVVNSRIVNQKASSGSFKVVAA 60

Query: 100 ESIPDENLEKQTSKDRWSGLGYDQSDDQQDITRGKGMVDSLFQAPMQSGTHYAVMSSYEY 159
           +++PDENLEKQTSKDRW GLGYDQSDDQQDITRGKGMVD+LFQAPMQSGTHYAVMSSYEY
Sbjct: 61  DALPDENLEKQTSKDRWRGLGYDQSDDQQDITRGKGMVDTLFQAPMQSGTHYAVMSSYEY 120

Query: 160 LSAGLKTYEGMDNLLDGYYIAPAFMDKLVVHITKNFMSLPNIKVPLILGLWGGKGQGKSF 219
           LS GLK+YEGMDNLLDGYYIAPAFMDKLVVHITKNFM+LPNIKVPLILGLWGGKGQGKSF
Sbjct: 121 LSTGLKSYEGMDNLLDGYYIAPAFMDKLVVHITKNFMTLPNIKVPLILGLWGGKGQGKSF 180

Query: 220 QCELVFAKMGVNPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG 279
           QCELVFAKMG+NPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG
Sbjct: 181 QCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG 240

Query: 280 AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAP 339
           AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAP
Sbjct: 241 AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAP 300

Query: 340 LIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARVY 399
           LIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARVY
Sbjct: 301 LIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARVY 360

Query: 400 DDEVRKWIIEIGVDSVGKKLVNSRDPPPKFEQPSMTLQKLLEYGSMLVQEQENVKRVQLA 459
           DDEVRKWI+ +GV  VGKKLVNSR+PPPKFEQP MTL+KLLEYG+MLVQEQENVKRVQLA
Sbjct: 361 DDEVRKWIVGVGVQDVGKKLVNSREPPPKFEQPKMTLEKLLEYGNMLVQEQENVKRVQLA 420

Query: 460 ETYLNEAALGNANEDAITRGAF 482
           +TYLNEAALGNANEDAI+RGAF
Sbjct: 421 DTYLNEAALGNANEDAISRGAF 442

BLAST of PI0000048 vs. NCBI nr
Match: XP_008461692.2 (PREDICTED: ribulose bisphosphate carboxylase/oxygenase activase 2, chloroplastic-like [Cucumis melo])

HSP 1 Score: 836.6 bits (2160), Expect = 1.0e-238
Identity = 417/442 (94.34%), Postives = 431/442 (97.51%), Query Frame = 0

Query: 40  MAASAASVGVVNHAPLSLNGSGSATSVPSSVFFGNSLKKVVNSRTVNAKASSGSFKVVAV 99
           MAASAASVGVVNHAPLSLNGSGSATSVPSSVFFG   KKVV SR V  KASSGSFKVVAV
Sbjct: 1   MAASAASVGVVNHAPLSLNGSGSATSVPSSVFFG---KKVVTSRAVTPKASSGSFKVVAV 60

Query: 100 ESIPDENLEKQTSKDRWSGLGYDQSDDQQDITRGKGMVDSLFQAPMQSGTHYAVMSSYEY 159
           ESIPDENLEKQT+KDRW+GLGYD SDDQQDITRGKGMVDSLFQAPMQSGTHYAVMSSYEY
Sbjct: 61  ESIPDENLEKQTNKDRWAGLGYDISDDQQDITRGKGMVDSLFQAPMQSGTHYAVMSSYEY 120

Query: 160 LSAGLKTYEGMDNLLDGYYIAPAFMDKLVVHITKNFMSLPNIKVPLILGLWGGKGQGKSF 219
           LS GLKTYEGMDN+LDGYYIAPAFMDKLVVHITKN+M+LPNIKVPLILGLWGGKGQGKSF
Sbjct: 121 LSTGLKTYEGMDNILDGYYIAPAFMDKLVVHITKNYMTLPNIKVPLILGLWGGKGQGKSF 180

Query: 220 QCELVFAKMGVNPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG 279
           QCELVFAKMG+NPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG
Sbjct: 181 QCELVFAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG 240

Query: 280 AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAP 339
           AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAP
Sbjct: 241 AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAP 300

Query: 340 LIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARVY 399
           LIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARVY
Sbjct: 301 LIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARVY 360

Query: 400 DDEVRKWIIEIGVDSVGKKLVNSRDPPPKFEQPSMTLQKLLEYGSMLVQEQENVKRVQLA 459
           DDEVRKW++ +GV ++GKKLVNS++PPPKFEQP+MTLQKLLEYGSMLVQEQENVKRVQLA
Sbjct: 361 DDEVRKWVVAVGVQAIGKKLVNSKEPPPKFEQPAMTLQKLLEYGSMLVQEQENVKRVQLA 420

Query: 460 ETYLNEAALGNANEDAITRGAF 482
           ETYLNEAALGNANEDAITRGAF
Sbjct: 421 ETYLNEAALGNANEDAITRGAF 439

BLAST of PI0000048 vs. NCBI nr
Match: XP_023529775.1 (ribulose bisphosphate carboxylase/oxygenase activase 2, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 810.1 bits (2091), Expect = 1.1e-230
Identity = 401/442 (90.72%), Postives = 423/442 (95.70%), Query Frame = 0

Query: 40  MAASAASVGVVNHAPLSLNGSGSATSVPSSVFFGNSLKKVVNSRTVNAKASSGSFKVVAV 99
           MAASAASVGV+NHAPLSLNGS SATSVPSSVFFG+SLKKVVNSR  + KAS GSFKVVA 
Sbjct: 1   MAASAASVGVINHAPLSLNGSSSATSVPSSVFFGSSLKKVVNSRIASPKASPGSFKVVAS 60

Query: 100 ESIPDENLEKQTSKDRWSGLGYDQSDDQQDITRGKGMVDSLFQAPMQSGTHYAVMSSYEY 159
           ++  DE+L+KQTSKDRW GL YD SDDQQDITRGKGMVD+LFQAPMQSGTH A+MSSYEY
Sbjct: 61  DAPADEDLDKQTSKDRWRGLAYDISDDQQDITRGKGMVDTLFQAPMQSGTHNAIMSSYEY 120

Query: 160 LSAGLKTYEGMDNLLDGYYIAPAFMDKLVVHITKNFMSLPNIKVPLILGLWGGKGQGKSF 219
           LS GLKTYEG+DNLLDG+YIAPAFMDKLVVHITKNFMSLPNIKVPLILG+WGGKGQGKSF
Sbjct: 121 LSTGLKTYEGLDNLLDGFYIAPAFMDKLVVHITKNFMSLPNIKVPLILGIWGGKGQGKSF 180

Query: 220 QCELVFAKMGVNPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG 279
           QCELVFAKMG+ PIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG
Sbjct: 181 QCELVFAKMGITPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG 240

Query: 280 AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAP 339
           AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKE+NPRVPIIVTGNDFSTLYAP
Sbjct: 241 AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEDNPRVPIIVTGNDFSTLYAP 300

Query: 340 LIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARVY 399
           LIRDGRMEKFYWAPTREDRIGVCKGIFRTDNV DED+VKLVDTFPGQSIDFFGALRARVY
Sbjct: 301 LIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVPDEDVVKLVDTFPGQSIDFFGALRARVY 360

Query: 400 DDEVRKWIIEIGVDSVGKKLVNSRDPPPKFEQPSMTLQKLLEYGSMLVQEQENVKRVQLA 459
           DDEVRKWII +GVD+VGKKLVNS+DPPPKFEQP MT++KLLEYG+MLVQEQENVKRVQLA
Sbjct: 361 DDEVRKWIIGVGVDTVGKKLVNSKDPPPKFEQPKMTVEKLLEYGNMLVQEQENVKRVQLA 420

Query: 460 ETYLNEAALGNANEDAITRGAF 482
           + YLNEAALGNANEDAI RGAF
Sbjct: 421 DKYLNEAALGNANEDAINRGAF 442

BLAST of PI0000048 vs. NCBI nr
Match: KAG6600039.1 (Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 808.9 bits (2088), Expect = 2.3e-230
Identity = 399/442 (90.27%), Postives = 424/442 (95.93%), Query Frame = 0

Query: 40  MAASAASVGVVNHAPLSLNGSGSATSVPSSVFFGNSLKKVVNSRTVNAKASSGSFKVVAV 99
           MAASAASVGV+NHAPLSLNGS SATSVPSSVFFG+SLKKVVNSR  ++KAS GSFKVVA 
Sbjct: 1   MAASAASVGVINHAPLSLNGSSSATSVPSSVFFGSSLKKVVNSRIASSKASPGSFKVVAS 60

Query: 100 ESIPDENLEKQTSKDRWSGLGYDQSDDQQDITRGKGMVDSLFQAPMQSGTHYAVMSSYEY 159
           ++  DE+L+KQTSKDRW GL YD SDDQQDITRGKGMVD+LFQAPMQSGTH A+MSSYEY
Sbjct: 61  DAPADEDLDKQTSKDRWRGLAYDISDDQQDITRGKGMVDTLFQAPMQSGTHNAIMSSYEY 120

Query: 160 LSAGLKTYEGMDNLLDGYYIAPAFMDKLVVHITKNFMSLPNIKVPLILGLWGGKGQGKSF 219
           LS GLKTYEG+DNLLDG+YIAPAFMDKLVVHITKNFMSLPNIKVPLILG+WGGKGQGKSF
Sbjct: 121 LSTGLKTYEGLDNLLDGFYIAPAFMDKLVVHITKNFMSLPNIKVPLILGIWGGKGQGKSF 180

Query: 220 QCELVFAKMGVNPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG 279
           QCELVFAKMG+ PIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG
Sbjct: 181 QCELVFAKMGITPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDAG 240

Query: 280 AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYAP 339
           AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKE+NPRVPIIVTGNDFSTLYAP
Sbjct: 241 AGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEDNPRVPIIVTGNDFSTLYAP 300

Query: 340 LIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARVY 399
           LIRDGRMEKFYWAPTREDRIGVCKGIFRTDNV DED+VKLVDTFPGQSIDFFGALRARVY
Sbjct: 301 LIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVPDEDVVKLVDTFPGQSIDFFGALRARVY 360

Query: 400 DDEVRKWIIEIGVDSVGKKLVNSRDPPPKFEQPSMTLQKLLEYGSMLVQEQENVKRVQLA 459
           DDEVRKWI+ +GVD+VGKKLVNS+DPPPKFEQP M+++KLLEYG+MLVQEQENVKRVQLA
Sbjct: 361 DDEVRKWIVGVGVDTVGKKLVNSKDPPPKFEQPKMSVEKLLEYGNMLVQEQENVKRVQLA 420

Query: 460 ETYLNEAALGNANEDAITRGAF 482
           + YLNEAALGNANEDAI RGAF
Sbjct: 421 DKYLNEAALGNANEDAINRGAF 442

BLAST of PI0000048 vs. TAIR 10
Match: AT2G39730.1 (rubisco activase )

HSP 1 Score: 692.2 bits (1785), Expect = 3.0e-199
Identity = 347/443 (78.33%), Postives = 387/443 (87.36%), Query Frame = 0

Query: 40  MAASAASVGVVNHAPLSLNGSGS-ATSVPSSVFFGNSLKKVVNSRTVNAKASSGSFKVVA 99
           MAA+ ++VG +N APLSLNGSGS A S P+S F G  +  V      N K S+GSFKV+A
Sbjct: 1   MAAAVSTVGAINRAPLSLNGSGSGAVSAPASTFLGKKVVTVSRFAQSN-KKSNGSFKVLA 60

Query: 100 VESIPDENLEKQTSKDRWSGLGYDQSDDQQDITRGKGMVDSLFQAPMQSGTHYAVMSSYE 159
           V+       +KQT  DRW GL YD SDDQQDITRGKGMVDS+FQAPM +GTH+AV+SSYE
Sbjct: 61  VKE------DKQTDGDRWRGLAYDTSDDQQDITRGKGMVDSVFQAPMGTGTHHAVLSSYE 120

Query: 160 YLSAGLKTYEGMDNLLDGYYIAPAFMDKLVVHITKNFMSLPNIKVPLILGLWGGKGQGKS 219
           Y+S GL+ Y  +DN++DG+YIAPAFMDKLVVHITKNF++LPNIKVPLILG+WGGKGQGKS
Sbjct: 121 YVSQGLRQY-NLDNMMDGFYIAPAFMDKLVVHITKNFLTLPNIKVPLILGIWGGKGQGKS 180

Query: 220 FQCELVFAKMGVNPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDA 279
           FQCELV AKMG+NPIMMSAGELESGNAGEPAKLIRQRYREAAD+IKKGKM CLFINDLDA
Sbjct: 181 FQCELVMAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIKKGKMCCLFINDLDA 240

Query: 280 GAGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYA 339
           GAGR+GGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEEN RVPII TGNDFSTLYA
Sbjct: 241 GAGRMGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENARVPIICTGNDFSTLYA 300

Query: 340 PLIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARV 399
           PLIRDGRMEKFYWAPTREDRIGVCKGIFRTD + DEDIV LVD FPGQSIDFFGALRARV
Sbjct: 301 PLIRDGRMEKFYWAPTREDRIGVCKGIFRTDKIKDEDIVTLVDQFPGQSIDFFGALRARV 360

Query: 400 YDDEVRKWIIEIGVDSVGKKLVNSRDPPPKFEQPSMTLQKLLEYGSMLVQEQENVKRVQL 459
           YDDEVRK++  +GV+ +GK+LVNSR+ PP FEQP MT +KL+EYG+MLV EQENVKRVQL
Sbjct: 361 YDDEVRKFVESLGVEKIGKRLVNSREGPPVFEQPEMTYEKLMEYGNMLVMEQENVKRVQL 420

Query: 460 AETYLNEAALGNANEDAITRGAF 482
           AETYL++AALG+AN DAI RG F
Sbjct: 421 AETYLSQAALGDANADAIGRGTF 435

BLAST of PI0000048 vs. TAIR 10
Match: AT2G39730.2 (rubisco activase )

HSP 1 Score: 692.2 bits (1785), Expect = 3.0e-199
Identity = 347/443 (78.33%), Postives = 387/443 (87.36%), Query Frame = 0

Query: 40  MAASAASVGVVNHAPLSLNGSGS-ATSVPSSVFFGNSLKKVVNSRTVNAKASSGSFKVVA 99
           MAA+ ++VG +N APLSLNGSGS A S P+S F G  +  V      N K S+GSFKV+A
Sbjct: 1   MAAAVSTVGAINRAPLSLNGSGSGAVSAPASTFLGKKVVTVSRFAQSN-KKSNGSFKVLA 60

Query: 100 VESIPDENLEKQTSKDRWSGLGYDQSDDQQDITRGKGMVDSLFQAPMQSGTHYAVMSSYE 159
           V+       +KQT  DRW GL YD SDDQQDITRGKGMVDS+FQAPM +GTH+AV+SSYE
Sbjct: 61  VKE------DKQTDGDRWRGLAYDTSDDQQDITRGKGMVDSVFQAPMGTGTHHAVLSSYE 120

Query: 160 YLSAGLKTYEGMDNLLDGYYIAPAFMDKLVVHITKNFMSLPNIKVPLILGLWGGKGQGKS 219
           Y+S GL+ Y  +DN++DG+YIAPAFMDKLVVHITKNF++LPNIKVPLILG+WGGKGQGKS
Sbjct: 121 YVSQGLRQY-NLDNMMDGFYIAPAFMDKLVVHITKNFLTLPNIKVPLILGIWGGKGQGKS 180

Query: 220 FQCELVFAKMGVNPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDA 279
           FQCELV AKMG+NPIMMSAGELESGNAGEPAKLIRQRYREAAD+IKKGKM CLFINDLDA
Sbjct: 181 FQCELVMAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIKKGKMCCLFINDLDA 240

Query: 280 GAGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYA 339
           GAGR+GGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEEN RVPII TGNDFSTLYA
Sbjct: 241 GAGRMGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENARVPIICTGNDFSTLYA 300

Query: 340 PLIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARV 399
           PLIRDGRMEKFYWAPTREDRIGVCKGIFRTD + DEDIV LVD FPGQSIDFFGALRARV
Sbjct: 301 PLIRDGRMEKFYWAPTREDRIGVCKGIFRTDKIKDEDIVTLVDQFPGQSIDFFGALRARV 360

Query: 400 YDDEVRKWIIEIGVDSVGKKLVNSRDPPPKFEQPSMTLQKLLEYGSMLVQEQENVKRVQL 459
           YDDEVRK++  +GV+ +GK+LVNSR+ PP FEQP MT +KL+EYG+MLV EQENVKRVQL
Sbjct: 361 YDDEVRKFVESLGVEKIGKRLVNSREGPPVFEQPEMTYEKLMEYGNMLVMEQENVKRVQL 420

Query: 460 AETYLNEAALGNANEDAITRGAF 482
           AETYL++AALG+AN DAI RG F
Sbjct: 421 AETYLSQAALGDANADAIGRGTF 435

BLAST of PI0000048 vs. TAIR 10
Match: AT2G39730.3 (rubisco activase )

HSP 1 Score: 692.2 bits (1785), Expect = 3.0e-199
Identity = 347/443 (78.33%), Postives = 387/443 (87.36%), Query Frame = 0

Query: 40  MAASAASVGVVNHAPLSLNGSGS-ATSVPSSVFFGNSLKKVVNSRTVNAKASSGSFKVVA 99
           MAA+ ++VG +N APLSLNGSGS A S P+S F G  +  V      N K S+GSFKV+A
Sbjct: 1   MAAAVSTVGAINRAPLSLNGSGSGAVSAPASTFLGKKVVTVSRFAQSN-KKSNGSFKVLA 60

Query: 100 VESIPDENLEKQTSKDRWSGLGYDQSDDQQDITRGKGMVDSLFQAPMQSGTHYAVMSSYE 159
           V+       +KQT  DRW GL YD SDDQQDITRGKGMVDS+FQAPM +GTH+AV+SSYE
Sbjct: 61  VKE------DKQTDGDRWRGLAYDTSDDQQDITRGKGMVDSVFQAPMGTGTHHAVLSSYE 120

Query: 160 YLSAGLKTYEGMDNLLDGYYIAPAFMDKLVVHITKNFMSLPNIKVPLILGLWGGKGQGKS 219
           Y+S GL+ Y  +DN++DG+YIAPAFMDKLVVHITKNF++LPNIKVPLILG+WGGKGQGKS
Sbjct: 121 YVSQGLRQY-NLDNMMDGFYIAPAFMDKLVVHITKNFLTLPNIKVPLILGIWGGKGQGKS 180

Query: 220 FQCELVFAKMGVNPIMMSAGELESGNAGEPAKLIRQRYREAADIIKKGKMSCLFINDLDA 279
           FQCELV AKMG+NPIMMSAGELESGNAGEPAKLIRQRYREAAD+IKKGKM CLFINDLDA
Sbjct: 181 FQCELVMAKMGINPIMMSAGELESGNAGEPAKLIRQRYREAADLIKKGKMCCLFINDLDA 240

Query: 280 GAGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENPRVPIIVTGNDFSTLYA 339
           GAGR+GGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEEN RVPII TGNDFSTLYA
Sbjct: 241 GAGRMGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEENARVPIICTGNDFSTLYA 300

Query: 340 PLIRDGRMEKFYWAPTREDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARV 399
           PLIRDGRMEKFYWAPTREDRIGVCKGIFRTD + DEDIV LVD FPGQSIDFFGALRARV
Sbjct: 301 PLIRDGRMEKFYWAPTREDRIGVCKGIFRTDKIKDEDIVTLVDQFPGQSIDFFGALRARV 360

Query: 400 YDDEVRKWIIEIGVDSVGKKLVNSRDPPPKFEQPSMTLQKLLEYGSMLVQEQENVKRVQL 459
           YDDEVRK++  +GV+ +GK+LVNSR+ PP FEQP MT +KL+EYG+MLV EQENVKRVQL
Sbjct: 361 YDDEVRKFVESLGVEKIGKRLVNSREGPPVFEQPEMTYEKLMEYGNMLVMEQENVKRVQL 420

Query: 460 AETYLNEAALGNANEDAITRGAF 482
           AETYL++AALG+AN DAI RG F
Sbjct: 421 AETYLSQAALGDANADAIGRGTF 435

BLAST of PI0000048 vs. TAIR 10
Match: AT1G73110.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 325.5 bits (833), Expect = 7.3e-89
Identity = 185/418 (44.26%), Postives = 263/418 (62.92%), Query Frame = 0

Query: 63  ATSVPSSVFFGNSLKKVVNSRTVNAKASSGSFKVVAVE---SIPDENLEKQTS------- 122
           ++S  SS F    +     S  V +K SS     VA +   + P + L +Q+S       
Sbjct: 17  SSSSSSSSFNATLINTRKLSSIVCSKPSSNDGGKVANDDGGAKPRKKLSEQSSWEVKDSE 76

Query: 123 -KDRWSGLGYDQSDDQQDITRGKGMVDSLFQAPMQSGTHYAVMSSYEYLSAGLKTYEGMD 182
            KD    LG +  +    +    GM+D +F           V   ++Y     +++E   
Sbjct: 77  GKDYLYRLGAESDNVNIAVGARSGMIDDVFIGDFLGKDSDIV---FDYRQKATRSFE--- 136

Query: 183 NLLDGYYIAPAFMDKLVVHITKNFMSLP-NIKVPLILGLWGGKGQGKSFQCELVFAKMGV 242
           +L   YYIAP+F+DK+ VHI KN+++   NIK+PLILG+WGGKGQGK+FQ EL+F  MGV
Sbjct: 137 HLQGDYYIAPSFLDKVAVHIVKNYLAPSLNIKIPLILGIWGGKGQGKTFQTELIFKTMGV 196

Query: 243 NPIMMSAGELESGNAGEPAKLIRQRYREAADIIK-KGKMSCLFINDLDAGAGRLGGTTQY 302
            P++MSAGELES  AGEP +LIR RYR A+ +I+ +GKMS L IND+DAG GR  G TQ 
Sbjct: 197 EPVIMSAGELESDRAGEPGRLIRDRYRTASQVIQNQGKMSVLMINDIDAGLGRF-GETQM 256

Query: 303 TVNNQMVNATLMNIADNPTNVQLPGMYNKEEN-PRVPIIVTGNDFSTLYAPLIRDGRMEK 362
           TVNNQ+V  TLMN+ADNPT V +   + + +   RVP+IVTGNDFSTLYAPLIR+GRMEK
Sbjct: 257 TVNNQIVVGTLMNLADNPTRVSVGQEWREADMVNRVPLIVTGNDFSTLYAPLIREGRMEK 316

Query: 363 FYWAPTREDRIGVCKGIFRTDNVADEDIVKLVDTFPGQSIDFFGALRARVYDDEVRKWII 422
           FYW PTRED + +   ++  D ++ +D++ +VD FP Q++DF+GALR+R YD  + KW+ 
Sbjct: 317 FYWQPTREDIVNIVSRMYEKDGISRKDVISIVDKFPNQALDFYGALRSRTYDRSILKWVD 376

Query: 423 EI-GVDSVGKKLVNSR--DPPPKFEQPSMTLQKLLEYGSMLVQEQENVKRVQLAETYL 464
           E  G++++GK L+  +     P+F  P  T++ LLE G  L+ EQ+ +   +L++ Y+
Sbjct: 377 EAGGMETLGKVLLRRKKTQEVPQFTAPEQTVEALLESGYSLINEQKLIMETKLSKEYM 427

BLAST of PI0000048 vs. TAIR 10
Match: AT2G03670.1 (cell division cycle 48B )

HSP 1 Score: 50.8 bits (120), Expect = 3.5e-06
Identity = 41/163 (25.15%), Postives = 72/163 (44.17%), Query Frame = 0

Query: 201 IKVPLILGLWGGKGQGKSFQCELVFAKMGVNPIMMSAGELESGNAGEPAKLIRQRYREAA 260
           +K P  L L+G  G GK+     V  +   + I++S   +   +AGE  K++R+ + EA+
Sbjct: 53  LKWPRGLLLYGPPGTGKTSLVRAVVQECDAHLIVLSPHSVHRAHAGESEKVLREAFAEAS 112

Query: 261 DIIKKGKMSCLFINDLDAGAGRLGGTTQYTVNNQMVNATLMNIADNPTNVQLPGMYNKEE 320
                 K S +FI+++D    R     +  V       TLM+ ++ P++           
Sbjct: 113 SHAVSDKPSVIFIDEIDVLCPRRDARREQDVRIASQLFTLMD-SNKPSS----------S 172

Query: 321 NPRVPIIVTGNDFSTLYAPLIRDGRMEKF--YWAPTREDRIGV 362
            PRV ++ + N    +   L R GR +       P  EDR+ +
Sbjct: 173 APRVVVVASTNRVDAIDPALRRAGRFDALVEVSTPNEEDRLKI 204

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q7X9A02.6e-20882.17Ribulose bisphosphate carboxylase/oxygenase activase 1, chloroplastic OS=Larrea ... [more]
Q7X9999.9e-20881.49Ribulose bisphosphate carboxylase/oxygenase activase 2, chloroplastic OS=Larrea ... [more]
O989971.1e-20681.00Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Vigna rad... [more]
Q402815.1e-20480.77Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Malus dom... [more]
O649811.9e-20380.54Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS=Phaseolus... [more]
Match NameE-valueIdentityDescription
A0A5A7U5268.1e-24592.51Ribulose bisphosphate carboxylase/oxygenase activase 2 OS=Cucumis melo var. maku... [more]
A0A1S3CF765.1e-23994.34ribulose bisphosphate carboxylase/oxygenase activase 2, chloroplastic-like OS=Cu... [more]
A0A6J1K8Q91.5e-23090.50ribulose bisphosphate carboxylase/oxygenase activase 2, chloroplastic-like OS=Cu... [more]
A0A0A0KLW91.5e-23091.20Beta-form rubisco activase OS=Cucumis sativus OX=3659 GN=Csa_5G182730 PE=4 SV=1[more]
A0A6J1FN785.6e-23090.05ribulose bisphosphate carboxylase/oxygenase activase 2, chloroplastic-like OS=Cu... [more]
Match NameE-valueIdentityDescription
KAA0050344.11.7e-24492.51ribulose bisphosphate carboxylase/oxygenase activase 2 [Cucumis melo var. makuwa... [more]
XP_038890850.12.9e-24194.80ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic-like [Beninc... [more]
XP_008461692.21.0e-23894.34PREDICTED: ribulose bisphosphate carboxylase/oxygenase activase 2, chloroplastic... [more]
XP_023529775.11.1e-23090.72ribulose bisphosphate carboxylase/oxygenase activase 2, chloroplastic-like [Cucu... [more]
KAG6600039.12.3e-23090.27Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic, partial [Cu... [more]
Match NameE-valueIdentityDescription
AT2G39730.13.0e-19978.33rubisco activase [more]
AT2G39730.23.0e-19978.33rubisco activase [more]
AT2G39730.33.0e-19978.33rubisco activase [more]
AT1G73110.17.3e-8944.26P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT2G03670.13.5e-0625.15cell division cycle 48B [more]
InterPro
Analysis Name: InterPro Annotations of Melon (PI 482460) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003959ATPase, AAA-type, corePFAMPF00004AAAcoord: 209..350
e-value: 1.2E-14
score: 54.9
NoneNo IPR availableGENE3D1.10.8.1070coord: 354..466
e-value: 1.7E-49
score: 168.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 109..128
NoneNo IPR availablePANTHERPTHR32429:SF25RIBULOSE BISPHOSPHATE CARBOXYLASE/OXYGENASE ACTIVASE, CHLOROPLASTIC-LIKE ISOFORM X1coord: 40..481
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 169..353
e-value: 8.7E-44
score: 151.3
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 173..385
IPR044960Ribulose bisphosphate carboxylase/oxygenase activase-likePANTHERPTHR32429FAMILY NOT NAMEDcoord: 40..481

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
PI0000048.1PI0000048.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0050790 regulation of catalytic activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity
molecular_function GO:0046863 ribulose-1,5-bisphosphate carboxylase/oxygenase activator activity