Moctig00543g110 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moctig00543g110
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Cytochrome b559 subunit alpha
Location	tig00000543_pilon: 42974 .. 44286 (+)
RNA-Seq Expression	Moctig00543g110
Synteny	Moctig00543g110

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTATGTACTACTGTTACTTGTTACTGGACGACTAACCAATTCCTTAAAATAAAAAAAAGATTCAAACAAAAAATATAACTACAAACACAAAAGGAATATTTCTAGATTTCACGTCTAATTTAATTTAATTTTGGAACTATGAGAATCTGCTTCCTGAATTATTATAAACCAACCAGTAGGATTCACATTTTACCCAATCTTCGGTTTCTAGCCCGAAGCCAATAACCGAGACAACCCTTATACTACTACAGGAATTTTCCGAATTTTATATTGAATGAATGGTACATGATTCTACATCGACAAGCACCAAGAAAAGAAGAAATTAATTATTTAGTGCTTCATTTCGATACTGATTGTGAAATTGCGTTGCTGCGTCAGAAGAAGGATAGCTATACTGATTCGGTATACTCTGAAAACACCCTCGGTACAATATTGACGATCTTACAAAGATAAAATTTCAGTTAATTTCCATTTACTGATCTCATCTTTTACGGAATCGATCACCTTTTGACTGTACAAGAATATGTGGAGCTCGGCATGTCTGGAAGCACAGGAGAACGTTCTTTTGCTGATATTATCACCAGTATTCGATACTGGGTCATTCATAGCATTACTATACCTTCTCTATTCATTGCAGGTTGGTTATTCGTCAGCACGGGTTTAGCTTACGATGTTTTTGGAAGCCCTCGTCCAAATGAGTATTTTACAGAGAGCCGACAAGGAATTCCATTAATAACTGGCCGTTTTGATTCTTTGGAACAACTCGATGAATTTAGTAGATCTTTTTAGGAGGACCAAATGACTATCGATAAAACTTATCCTATTTTTACAGTGCGATGGTTGGCTGTTCACGGACTAGCTGTACCTACCGTTTCTTTTTTAGGGTCAATATCAGCAATGCAGTTCATCCAACGATAAACCTAATCCGAATTATAGAGCTATGACACAATCAAATCCGAACGAACAAAATGTTGAATTGAATCGTACCAGTCTCTACTGGGGGTTATTACTCATTTTTGTACTTGCTGTTTTATTTTCCAATTATTTCTTCAATTAAATAAAAGAAAATACTAAATAATAGTACGAATTATCTTATCCCATTCGGAAGGATATCATCTCATAATTATCTATGACTGTTTCTGTCTCTAGCACGACCACTTGATGAAATGTGGAGGGAAGTGGGACAAATGGCCGAGACTACTGGAAGGATTCCTCTTTGGATAATAGGTACTGTAACTGGTATTACTGTGATCGGTTTAATCGGTATTTTCTTTTATGGTTCATATTCTGGATTAGGTTCATCCCTGTAA

mRNA sequence

ATGAAGAAGGATAGCTATACTGATTCGGTATACTCTGAAAACACCCTCGAATATGTGGAGCTCGGCATGTCTGGAAGCACAGGAGAACGTTCTTTTGCTGATATTATCACCAGTATTCGATACTGGGTCATTCATAGCATTACTATACCTTCTCTATTCATTGCAGCACGACCACTTGATGAAATGTGGAGGGAAGTGGGACAAATGGCCGAGACTACTGGAAGGATTCCTCTTTGGATAATAGGTTCATCCCTGTAA

Coding sequence (CDS)

ATGAAGAAGGATAGCTATACTGATTCGGTATACTCTGAAAACACCCTCGAATATGTGGAGCTCGGCATGTCTGGAAGCACAGGAGAACGTTCTTTTGCTGATATTATCACCAGTATTCGATACTGGGTCATTCATAGCATTACTATACCTTCTCTATTCATTGCAGCACGACCACTTGATGAAATGTGGAGGGAAGTGGGACAAATGGCCGAGACTACTGGAAGGATTCCTCTTTGGATAATAGGTTCATCCCTGTAA

Protein sequence

MKKDSYTDSVYSENTLEYVELGMSGSTGERSFADIITSIRYWVIHSITIPSLFIAARPLDEMWREVGQMAETTGRIPLWIIGSSL

Homology

[Close]

BLAST of Moctig00543g110 vs. NCBI nr
Match: QCE13742.1 (photosystem II cytochrome b559 subunit alpha [Vigna unguiculata])

HSP 1 Score: 104.4 bits (259), Expect = 5.0e-19
Identity = 51/54 (94.44%), Postives = 52/54 (96.30%), Query Frame = 0


Query: 2   KKDSYTDSVYSENTLEYVELGMSGSTGERSFADIITSIRYWVIHSITIPSLFIA 56
           KKDSYTDSVYS+NTLEY EL MSGSTGERSFADIITSIRYWVIHSITIPSLFIA
Sbjct: 132 KKDSYTDSVYSKNTLEYAELNMSGSTGERSFADIITSIRYWVIHSITIPSLFIA 185

[Close]

BLAST of Moctig00543g110 vs. NCBI nr
Match: KAF4396260.1 (hypothetical protein F8388_019806 [Cannabis sativa])

HSP 1 Score: 103.2 bits (256), Expect = 1.1e-18
Identity = 68/160 (42.50%), Postives = 73/160 (45.62%), Query Frame = 0


Query: 2   KKDSYTDSVYSENTL--------------------------------EYVELGMSGSTGE 61
           KK+SYTDSVYS+ TL                                EY EL M GS GE
Sbjct: 27  KKNSYTDSVYSKETLGTLLTISQRSDFSEFLFTDFIFYGVDPPLTVQEYAELKMFGSIGE 86

Query: 62  RSFADIITSIRYWVIHSITIPSLFIA---------------------------------- 84
           RSFADIITSIRYWVIHSITIPSLFI                                   
Sbjct: 87  RSFADIITSIRYWVIHSITIPSLFITGWLFVSTGLAYDVFGSPRPNEYFTESRQGVPLIT 146

[Close]

BLAST of Moctig00543g110 vs. NCBI nr
Match: PHU30796.1 (Cytochrome subunit alpha [Capsicum chinense])

HSP 1 Score: 99.8 bits (247), Expect = 1.2e-17
Identity = 49/54 (90.74%), Postives = 51/54 (94.44%), Query Frame = 0


Query: 2   KKDSYTDSVYSENTLEYVELGMSGSTGERSFADIITSIRYWVIHSITIPSLFIA 56
           KKDSYTDSVYS++ LEYVEL MSGS GERSFADIITSIRYWVIHSITIPSLFIA
Sbjct: 96  KKDSYTDSVYSKDALEYVELSMSGSIGERSFADIITSIRYWVIHSITIPSLFIA 149

[Close]

BLAST of Moctig00543g110 vs. NCBI nr
Match: TYI86979.1 (hypothetical protein E1A91_D04G105100v1 [Gossypium mustelinum])

HSP 1 Score: 91.7 bits (226), Expect = 3.4e-15
Identity = 52/84 (61.90%), Postives = 54/84 (64.29%), Query Frame = 0


Query: 2   KKDSYTDSVYSENTL-----------------------------EYVELGMSGSTGERSF 57
           KKDSYTDSVYS+NT                              EYVELGMSGSTGERSF
Sbjct: 60  KKDSYTDSVYSKNTFGTILTISQILSFSEFPFTDPIFYGIDPLQEYVELGMSGSTGERSF 119

[Close]

BLAST of Moctig00543g110 vs. NCBI nr
Match: TYI97217.1 (hypothetical protein E1A91_D01G127700v1 [Gossypium mustelinum])

HSP 1 Score: 91.3 bits (225), Expect = 4.4e-15
Identity = 52/83 (62.65%), Postives = 53/83 (63.86%), Query Frame = 0


Query: 2   KKDSYTDSVYSENTL-----------------------------EYVELGMSGSTGERSF 56
           KKDSYTDSVYS+NT                              EYVELGMSGSTGERSF
Sbjct: 60  KKDSYTDSVYSKNTFGTILTISQRLSFGEFPFTDPIFYGIDPLQEYVELGMSGSTGERSF 119

[Close]

BLAST of Moctig00543g110 vs. ExPASy Swiss-Prot
Match: Q3V518 (Cytochrome b559 subunit alpha OS=Acorus calamus OX=4465 GN=psbE PE=3 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 4.0e-11
Identity = 33/33 (100.00%), Postives = 33/33 (100.00%), Query Frame = 0


Query: 23 MSGSTGERSFADIITSIRYWVIHSITIPSLFIA 56
          MSGSTGERSFADIITSIRYWVIHSITIPSLFIA
Sbjct: 1  MSGSTGERSFADIITSIRYWVIHSITIPSLFIA 33

[Close]

BLAST of Moctig00543g110 vs. ExPASy Swiss-Prot
Match: A1EA25 (Cytochrome b559 subunit alpha OS=Agrostis stolonifera OX=63632 GN=psbE PE=3 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 4.0e-11
Identity = 33/33 (100.00%), Postives = 33/33 (100.00%), Query Frame = 0


Query: 23 MSGSTGERSFADIITSIRYWVIHSITIPSLFIA 56
          MSGSTGERSFADIITSIRYWVIHSITIPSLFIA
Sbjct: 1  MSGSTGERSFADIITSIRYWVIHSITIPSLFIA 33

[Close]

BLAST of Moctig00543g110 vs. ExPASy Swiss-Prot
Match: Q70XY9 (Cytochrome b559 subunit alpha OS=Amborella trichopoda OX=13333 GN=psbE PE=3 SV=3)

HSP 1 Score: 68.6 bits (166), Expect = 4.0e-11
Identity = 33/33 (100.00%), Postives = 33/33 (100.00%), Query Frame = 0


Query: 23 MSGSTGERSFADIITSIRYWVIHSITIPSLFIA 56
          MSGSTGERSFADIITSIRYWVIHSITIPSLFIA
Sbjct: 1  MSGSTGERSFADIITSIRYWVIHSITIPSLFIA 33

[Close]

BLAST of Moctig00543g110 vs. ExPASy Swiss-Prot
Match: P56779 (Cytochrome b559 subunit alpha OS=Arabidopsis thaliana OX=3702 GN=psbE PE=1 SV=4)

HSP 1 Score: 68.6 bits (166), Expect = 4.0e-11
Identity = 33/33 (100.00%), Postives = 33/33 (100.00%), Query Frame = 0


Query: 23 MSGSTGERSFADIITSIRYWVIHSITIPSLFIA 56
          MSGSTGERSFADIITSIRYWVIHSITIPSLFIA
Sbjct: 1  MSGSTGERSFADIITSIRYWVIHSITIPSLFIA 33

[Close]

BLAST of Moctig00543g110 vs. ExPASy Swiss-Prot
Match: P59702 (Cytochrome b559 subunit alpha OS=Atropa belladonna OX=33113 GN=psbE PE=3 SV=3)

HSP 1 Score: 68.6 bits (166), Expect = 4.0e-11
Identity = 33/33 (100.00%), Postives = 33/33 (100.00%), Query Frame = 0


Query: 23 MSGSTGERSFADIITSIRYWVIHSITIPSLFIA 56
          MSGSTGERSFADIITSIRYWVIHSITIPSLFIA
Sbjct: 1  MSGSTGERSFADIITSIRYWVIHSITIPSLFIA 33

[Close]

BLAST of Moctig00543g110 vs. ExPASy TrEMBL
Match: A0A4D6NL22 (Cytochrome b559 subunit alpha OS=Vigna unguiculata OX=3917 GN=DEO72_LG11g738 PE=3 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 2.4e-19
Identity = 51/54 (94.44%), Postives = 52/54 (96.30%), Query Frame = 0


Query: 2   KKDSYTDSVYSENTLEYVELGMSGSTGERSFADIITSIRYWVIHSITIPSLFIA 56
           KKDSYTDSVYS+NTLEY EL MSGSTGERSFADIITSIRYWVIHSITIPSLFIA
Sbjct: 132 KKDSYTDSVYSKNTLEYAELNMSGSTGERSFADIITSIRYWVIHSITIPSLFIA 185

[Close]

BLAST of Moctig00543g110 vs. ExPASy TrEMBL
Match: A0A7J6HPK4 (Cytochrome b559 subunit alpha OS=Cannabis sativa OX=3483 GN=F8388_019806 PE=3 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 5.4e-19
Identity = 68/160 (42.50%), Postives = 73/160 (45.62%), Query Frame = 0


Query: 2   KKDSYTDSVYSENTL--------------------------------EYVELGMSGSTGE 61
           KK+SYTDSVYS+ TL                                EY EL M GS GE
Sbjct: 27  KKNSYTDSVYSKETLGTLLTISQRSDFSEFLFTDFIFYGVDPPLTVQEYAELKMFGSIGE 86

Query: 62  RSFADIITSIRYWVIHSITIPSLFIA---------------------------------- 84
           RSFADIITSIRYWVIHSITIPSLFI                                   
Sbjct: 87  RSFADIITSIRYWVIHSITIPSLFITGWLFVSTGLAYDVFGSPRPNEYFTESRQGVPLIT 146

[Close]

BLAST of Moctig00543g110 vs. ExPASy TrEMBL
Match: A0A2G3DIH4 (Cytochrome b559 subunit alpha OS=Capsicum chinense OX=80379 GN=BC332_02889 PE=3 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 6.0e-18
Identity = 49/54 (90.74%), Postives = 51/54 (94.44%), Query Frame = 0


Query: 2   KKDSYTDSVYSENTLEYVELGMSGSTGERSFADIITSIRYWVIHSITIPSLFIA 56
           KKDSYTDSVYS++ LEYVEL MSGS GERSFADIITSIRYWVIHSITIPSLFIA
Sbjct: 96  KKDSYTDSVYSKDALEYVELSMSGSIGERSFADIITSIRYWVIHSITIPSLFIA 149

[Close]

BLAST of Moctig00543g110 vs. ExPASy TrEMBL
Match: A0A5D2VCD2 (Cytochrome b559 subunit alpha OS=Gossypium mustelinum OX=34275 GN=E1A91_D04G105100v1 PE=3 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 1.6e-15
Identity = 52/84 (61.90%), Postives = 54/84 (64.29%), Query Frame = 0


Query: 2   KKDSYTDSVYSENTL-----------------------------EYVELGMSGSTGERSF 57
           KKDSYTDSVYS+NT                              EYVELGMSGSTGERSF
Sbjct: 60  KKDSYTDSVYSKNTFGTILTISQILSFSEFPFTDPIFYGIDPLQEYVELGMSGSTGERSF 119

[Close]

BLAST of Moctig00543g110 vs. ExPASy TrEMBL
Match: A0A5J5NCB9 (Cytochrome b559 subunit alpha OS=Gossypium barbadense OX=3634 GN=ES319_1Z018000v1 PE=3 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 2.1e-15
Identity = 52/83 (62.65%), Postives = 53/83 (63.86%), Query Frame = 0


Query: 2   KKDSYTDSVYSENTL-----------------------------EYVELGMSGSTGERSF 56
           KKDSYTDSVYS+NT                              EYVELGMSGSTGERSF
Sbjct: 60  KKDSYTDSVYSKNTFGTILTISQRLSFGEFPFTNPIFYGIDPLQEYVELGMSGSTGERSF 119

[Close]

BLAST of Moctig00543g110 vs. TAIR 10
Match: ATCG00580.1 (photosystem II reaction center protein E )

HSP 1 Score: 68.6 bits (166), Expect = 2.8e-12
Identity = 33/33 (100.00%), Postives = 33/33 (100.00%), Query Frame = 0


Query: 23 MSGSTGERSFADIITSIRYWVIHSITIPSLFIA 56
          MSGSTGERSFADIITSIRYWVIHSITIPSLFIA
Sbjct: 1  MSGSTGERSFADIITSIRYWVIHSITIPSLFIA 33

The following BLAST results are available for this feature:

BLAST of Moctig00543g110 vs. NCBI nr


Match Name	E-value	Identity	Description
QCE13742.1	5.0e-19	94.44	photosystem II cytochrome b559 subunit alpha [Vigna unguiculata]	[more]
KAF4396260.1	1.1e-18	42.50	hypothetical protein F8388_019806 [Cannabis sativa]	[more]
PHU30796.1	1.2e-17	90.74	Cytochrome subunit alpha [Capsicum chinense]	[more]
TYI86979.1	3.4e-15	61.90	hypothetical protein E1A91_D04G105100v1 [Gossypium mustelinum]	[more]
TYI97217.1	4.4e-15	62.65	hypothetical protein E1A91_D01G127700v1 [Gossypium mustelinum]	[more]

BLAST of Moctig00543g110 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q3V518	4.0e-11	100.00	Cytochrome b559 subunit alpha OS=Acorus calamus OX=4465 GN=psbE PE=3 SV=1	[more]
A1EA25	4.0e-11	100.00	Cytochrome b559 subunit alpha OS=Agrostis stolonifera OX=63632 GN=psbE PE=3 SV=1	[more]
Q70XY9	4.0e-11	100.00	Cytochrome b559 subunit alpha OS=Amborella trichopoda OX=13333 GN=psbE PE=3 SV=3	[more]
P56779	4.0e-11	100.00	Cytochrome b559 subunit alpha OS=Arabidopsis thaliana OX=3702 GN=psbE PE=1 SV=4	[more]
P59702	4.0e-11	100.00	Cytochrome b559 subunit alpha OS=Atropa belladonna OX=33113 GN=psbE PE=3 SV=3	[more]

BLAST of Moctig00543g110 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A4D6NL22	2.4e-19	94.44	Cytochrome b559 subunit alpha OS=Vigna unguiculata OX=3917 GN=DEO72_LG11g738 PE=...	[more]
A0A7J6HPK4	5.4e-19	42.50	Cytochrome b559 subunit alpha OS=Cannabis sativa OX=3483 GN=F8388_019806 PE=3 SV...	[more]
A0A2G3DIH4	6.0e-18	90.74	Cytochrome b559 subunit alpha OS=Capsicum chinense OX=80379 GN=BC332_02889 PE=3 ...	[more]
A0A5D2VCD2	1.6e-15	61.90	Cytochrome b559 subunit alpha OS=Gossypium mustelinum OX=34275 GN=E1A91_D04G1051...	[more]
A0A5J5NCB9	2.1e-15	62.65	Cytochrome b559 subunit alpha OS=Gossypium barbadense OX=3634 GN=ES319_1Z018000v...	[more]

BLAST of Moctig00543g110 vs. TAIR 10


Match Name	E-value	Identity	Description
ATCG00580.1	2.8e-12	100.00	photosystem II reaction center protein E	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013081	Photosystem II cytochrome b559, N-terminal	PFAM	PF00283	Cytochrom_B559	coord: 28..55 e-value: 2.3E-14 score: 52.5
IPR037025	Photosystem II cytochrome b559, alpha subunit superfamily	GENE3D	1.20.5.860	Photosystem II cytochrome b559, alpha subunit	coord: 24..58 e-value: 5.0E-17 score: 63.4
None	No IPR available	PANTHER	PTHR33391:SF15	CYTOCHROME B559 SUBUNIT ALPHA	coord: 23..56
None	No IPR available	PANTHER	PTHR33391	CYTOCHROME B559 SUBUNIT BETA-RELATED	coord: 23..56
None	No IPR available	SUPERFAMILY	161045	Cytochrome b559 subunits	coord: 25..56
IPR006216	Photosystem II cytochrome b559, conserved site	PROSITE	PS00537	CYTOCHROME_B559	coord: 36..50

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moctig00543g110.1	Moctig00543g110.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009767	photosynthetic electron transport chain
biological_process	GO:0006412	translation
biological_process	GO:0015979	photosynthesis
biological_process	GO:0019684	photosynthesis, light reaction
cellular_component	GO:0009535	chloroplast thylakoid membrane
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0009539	photosystem II reaction center
cellular_component	GO:0005840	ribosome
cellular_component	GO:0009523	photosystem II
molecular_function	GO:0020037	heme binding
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0019843	rRNA binding
molecular_function	GO:0003735	structural constituent of ribosome