Moc11g31620 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc11g31620
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionRNase H domain-containing protein
Locationchr11: 23080931 .. 23082271 (-)
RNA-Seq ExpressionMoc11g31620
SyntenyMoc11g31620
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTTTTAAAAATTTGGAGTTGTTTAATCAATCTTTATTGGCTAAGCAGGATTGGCGGTTGCTGCGAGACCCTACATCATTGCTTGCCCGTGTTCTACAGGGTTGTTATTTCCGGGATGGTAGCTTTATGACTGCTCGTCTTGGGTGAAATCCATCCTTTATTTGGCGTAGCCTTTTGTGGGGTCAGGATCTGCTTTGTAAGGGGTTGCGTTGGTCTATGGGTAATTGGAAATCCATTCCTGTTTATAGTGCTAACTTGCTTCCTAGAGAGGGCTCGCTTCAAGTTCGGTCTTTGCAACGTCTTCCTATCTATACTAGAGTTTGTGATTTGTTTACAGCTTCGGGTAGGTGGAATGAACAATTGATTCGGAGAAGCTTTGACCTTGAGGAAGCCCAGATTATTTTAGCTATTTCATTGCCTCATCTTCATGTTTCGGACCACCTTATCTGACATTTTTTTATAATGTTGGTATCTTCTTCGTTAAAAGCAGATATCGGGTGGCCCAGCAACAGGATTTGTTGTCTGTATCGTTCTCTTCTTTAAGGGACACCCTTCTGCAATGGTGGAAAGACTTTTAAAACATGCACCTCCCTACTAAAATTAAAATATTTATGTGGCGTCTGTATGGTAATCGACTCCCCATGTGCGAGGCCCTTGTCTGGAGGGGGTTGGCTATTCCGAACTTCTGTTACTTTGTGGAGGTGCCAGTGAGGGTGTCCTTCATCTTTTTTGGTTTTGTGATGTTGTGCAGTTGTTCTGGTTGAACTCGCAATTTGCTCACCTTCGACACTCTTGGCCTGCCCCGTCTATCTCCTTCTTGCTTGTCGATTTGCAGGAGAGAGTCTATTGGCAGGTTTTTTAGGAGCTATCTGTCTTTCTGTAGGCAGTGTGGATTTCGCGTATCAAACTATACACCAGACGTGTGGGTCCTTTGGGCCCACTGGGAAGTTGGGACAAGACACTCAGCATTTCCGATCAGTGACGGCGATTCATTCTCCTATTGGGTCTTTATGTACAAGGCGGAGTTTGTACAGATGGCAGCCCCCCGCAGATGGTTGGGTGAAGGTCAATACTAATGCGTCTTTTTATGCAGCTTCTTTGACGGGGGGTTTAGATGTGTTGACTCGTGATTCACATGATATTGTCCTTCTTGCTGCCACGTTTTATATTCCCCATTGTGCTTCAGTGGATTGGGCAGAGGCTTTGGCGATCAAGGAGGGGTTGGCATTAGCGATTGATATGGGTGTTCGTTCGTTTCTGCTGGAAACTGATTCCTTATGCTGTTTTTGGTTGTTGGATCGGCCTGTGGATGATTGGAAGTTGGTGCTATCCTCTTAG

mRNA sequence

ATGAGTTTTAAAAATTTGGAGTTGTTTAATCAATCTTTATTGGCTAAGCAGGATTGGCGGTTGCTGCGAGACCCTACATCATTGCTTGCCCGTGTTCTACAGGGTTGTTATTTCCGGGATGGTAGCTTTATGACTGCTCGTCTTGGTGCTAACTTGCTTCCTAGAGAGGGCTCGCTTCAAGTTCGGTCTTTGCAACGTCTTCCTATCTATACTAGAGTTTGTGATTTGTTTACAGCTTCGGTTGTTCTGGTTGAACTCGCAATTTGCTCACCTTCGACACTCTTGGCCTGCCCCGTCTATCTCCTTCTTGCTTGTCGATTTGCAGGAGAGAGTCTATTGGCAGGTTTTTTAGGAGCTATCTGTCTTTCTGTAGGCAGTGTGGATTTCGCGTATCAAACTATACACCAGACGTGTGGGTCCTTTGGGCCCACTGGGAAGTTGGGACAAGACACTCAGCATTTCCGATCAGTGACGGCGATTCATTCTCCTATTGGGTCTTTATGTACAAGGCGGAGTTTGTACAGATGGCAGCCCCCCGCAGATGGTTGGGTGAAGGTCAATACTAATGCGTCTTTTTATGCAGCTTCTTTGACGGGGGGTTTAGATGTGTTGACTCGTGATTCACATGATATTGTCCTTCTTGCTGCCACGTTTTATATTCCCCATTGTGCTTCAGTGGATTGGGCAGAGGCTTTGGCGATCAAGGAGGGGTTGGCATTAGCGATTGATATGGGTGTTCGTTCGTTTCTGCTGGAAACTGATTCCTTATGCTGTTTTTGGTTGTTGGATCGGCCTGTGGATGATTGGAAGTTGGTGCTATCCTCTTAG

Coding sequence (CDS)

ATGAGTTTTAAAAATTTGGAGTTGTTTAATCAATCTTTATTGGCTAAGCAGGATTGGCGGTTGCTGCGAGACCCTACATCATTGCTTGCCCGTGTTCTACAGGGTTGTTATTTCCGGGATGGTAGCTTTATGACTGCTCGTCTTGGTGCTAACTTGCTTCCTAGAGAGGGCTCGCTTCAAGTTCGGTCTTTGCAACGTCTTCCTATCTATACTAGAGTTTGTGATTTGTTTACAGCTTCGGTTGTTCTGGTTGAACTCGCAATTTGCTCACCTTCGACACTCTTGGCCTGCCCCGTCTATCTCCTTCTTGCTTGTCGATTTGCAGGAGAGAGTCTATTGGCAGGTTTTTTAGGAGCTATCTGTCTTTCTGTAGGCAGTGTGGATTTCGCGTATCAAACTATACACCAGACGTGTGGGTCCTTTGGGCCCACTGGGAAGTTGGGACAAGACACTCAGCATTTCCGATCAGTGACGGCGATTCATTCTCCTATTGGGTCTTTATGTACAAGGCGGAGTTTGTACAGATGGCAGCCCCCCGCAGATGGTTGGGTGAAGGTCAATACTAATGCGTCTTTTTATGCAGCTTCTTTGACGGGGGGTTTAGATGTGTTGACTCGTGATTCACATGATATTGTCCTTCTTGCTGCCACGTTTTATATTCCCCATTGTGCTTCAGTGGATTGGGCAGAGGCTTTGGCGATCAAGGAGGGGTTGGCATTAGCGATTGATATGGGTGTTCGTTCGTTTCTGCTGGAAACTGATTCCTTATGCTGTTTTTGGTTGTTGGATCGGCCTGTGGATGATTGGAAGTTGGTGCTATCCTCTTAG

Protein sequence

MSFKNLELFNQSLLAKQDWRLLRDPTSLLARVLQGCYFRDGSFMTARLGANLLPREGSLQVRSLQRLPIYTRVCDLFTASVVLVELAICSPSTLLACPVYLLLACRFAGESLLAGFLGAICLSVGSVDFAYQTIHQTCGSFGPTGKLGQDTQHFRSVTAIHSPIGSLCTRRSLYRWQPPADGWVKVNTNASFYAASLTGGLDVLTRDSHDIVLLAATFYIPHCASVDWAEALAIKEGLALAIDMGVRSFLLETDSLCCFWLLDRPVDDWKLVLSS
Homology
BLAST of Moc11g31620 vs. NCBI nr
Match: XP_024196130.1 (uncharacterized protein LOC112199331 [Rosa chinensis])

HSP 1 Score: 82.8 bits (203), Expect = 5.1e-12
Identity = 81/306 (26.47%), Postives = 129/306 (42.16%), Query Frame = 0

Query: 1   MSFKNLELFNQSLLAKQDWRLLRDPTSLLARVLQGCYFRDGSFMTARLG----------- 60
           M FKNL  FN+++LAKQ WRLL +P SL+AR+ +  YF +G ++TA LG           
Sbjct: 352 MGFKNLHWFNKAMLAKQSWRLLHNPNSLIARLYKAIYFPNGEYLTAGLGDTPSFSWRSIV 411

Query: 61  --ANLLPREGSLQVRSLQRLPIYTR--VCDLFTASVVLVELAICSPSTLLACPVYLLLAC 120
               +L +    QV + +R+ I+    + D++             P    + P Y   A 
Sbjct: 412 EARQVLVQGLRWQVGNGERIEIWNHNWIPDVY-------------PRGPSSPPPY--DAP 471

Query: 121 RFAGE-----------SLLAG------FLGAICLSVGSVDFAYQTIHQTCGSFGPTG--- 180
           RF  E           +LLA          A+  ++       + +    G   PTG   
Sbjct: 472 RFVEELIDPITRSWDVNLLASPPPPVDSYKAVWKAIWDAKIPSKEVWHRAGLQLPTGPGD 531

Query: 181 -------KLGQDTQHFRSVTAIHS----------PIGSLCTRRSLYRWQPPADGWVKVNT 240
                  +L +  +   S   + +           +     RR L +W  P + WVKVN 
Sbjct: 532 NFIDWNSQLWEHKKQHASEAVLLTMGWYEEFKKVNVQPTDIRRQLVKWTRPQENWVKVNC 591

Query: 241 NASFYAASLTGGLDVLTRDSHDIVLLAATFYIPHCASVDWAEALAIKEGLALAIDMGVRS 255
           + +F  A+  GG  V+ RD++    + A   +P   S   AE +A+KEG+ LA+ +    
Sbjct: 592 DGAFQPATRKGGAGVVIRDANGAFQVGAARLLPLVTSPFHAELMALKEGINLAVALQHEQ 642

BLAST of Moc11g31620 vs. NCBI nr
Match: XP_022150918.1 (uncharacterized protein LOC111018954 [Momordica charantia])

HSP 1 Score: 75.5 bits (184), Expect = 8.1e-10
Identity = 93/420 (22.14%), Postives = 145/420 (34.52%), Query Frame = 0

Query: 1    MSFKNLELFNQSLLAKQDWRLLRDPTSLLARVLQGCYFRDGSFMTARL------------ 60
            M F++LELFN++LLAKQ WR+L  P S+L+RVL+G YF+D SFM A++            
Sbjct: 646  MGFRDLELFNKALLAKQCWRILNHPNSMLSRVLKGRYFKDCSFMEAKISGNPSYIWRSIL 705

Query: 61   -----------------------GANLLPREGSLQVRSLQRLPIYTRVCDL-------FT 120
                                   G N +P + +L++ S  RLP+ +RV  L       + 
Sbjct: 706  WGRDLLKKGLRWRIGNGDSVFIYGDNWVPNQPTLKILSSPRLPLVSRVSSLVDHEEGGWQ 765

Query: 121  ASVVLVELAICSPSTLLACP---------------------------------------- 180
              VV  E        +L+ P                                        
Sbjct: 766  GDVVRDEFTPDEAKGILSIPIGRGAEEDRLIWNYEKTGVYSVRSGYKVALLNNPCVQAPS 825

Query: 181  ------------------------------------------------------------ 240
                                                                        
Sbjct: 826  SSSSEEVRCWWNGFWKMHIPNKIKVFLWRLCLDRLPTGCNLSKRGVEITNCCYFCGRNGE 885

Query: 241  --VYLLLACRFAGESLLAGFLGAIC---------LSVGSVDFAYQTI-------HQTCGS 251
              ++L   C+FA    +    G +           S+   DF    +        +   +
Sbjct: 886  DSIHLFWICKFAEALWINSKFGKLSPFLILRESHESLSKADFEELCVVIWGLWNQRNARA 945

BLAST of Moc11g31620 vs. NCBI nr
Match: KAE8711725.1 (hypothetical protein F3Y22_tig00110279pilonHSYRG00089 [Hibiscus syriacus])

HSP 1 Score: 75.1 bits (183), Expect = 1.1e-09
Identity = 78/268 (29.10%), Postives = 119/268 (44.40%), Query Frame = 0

Query: 1   MSFKNLELFNQSLLAKQDWRLLRDPTSLLARVLQGCYFRDGSFMTARLGANLLPREGSLQ 60
           +  ++LE+FNQ+ L KQ WRL     +L+A+VL+  Y+ DGSF+TA L   ++    S++
Sbjct: 82  LGIRDLEVFNQAFLCKQGWRLFNGSKTLVAQVLKAKYYPDGSFLTADLEVLIIFFYVSMR 141

Query: 61  VRSLQRLPIYTRVCDLFTASVVLVELAICSPSTLLACPVYLLL-ACRFAGESLLA----- 120
              LQ    +  + + F  +    ELA       L C + L+  +C   G  L+      
Sbjct: 142 NERLQMPGTFISI-ECFGHNRPREELA-----KSLFCVLSLVAPSCTRRGGCLVGSSSPR 201

Query: 121 ---GFLGAIC-LSVGSVDF-----AYQTIHQTCGSFGPTGKLGQDTQHFRSVTAIHSPIG 180
              G  G +C LS+    F      +   + + GSF   G  G                 
Sbjct: 202 VTKGTSGNVCFLSLPGPYFLGCGDPWVCDYLSRGSFFGVGSPG----------------- 261

Query: 181 SLCTRRSLYR-WQPPADGWVKVNTNASFYAASLTGGLDVLTRDSHDIVLLAATFYIPHCA 240
             C    + R W  P  G++KVN + S+     TGG+  + RDS    L   +  IPH  
Sbjct: 262 --CGSYPIVRLWTIPPAGFIKVNVDGSYDKDLRTGGICCVLRDSTGQFLAGCSSGIPHAM 321

Query: 241 SVDWAEALAIKEGLALAIDMGVRSFLLE 253
                E+LA  EGL LA+ +G RS ++E
Sbjct: 322 DAFPVESLACLEGLNLALSLGCRSLVVE 324

BLAST of Moc11g31620 vs. NCBI nr
Match: XP_022135942.1 (uncharacterized protein LOC111007775 [Momordica charantia])

HSP 1 Score: 74.3 bits (181), Expect = 1.8e-09
Identity = 41/93 (44.09%), Postives = 56/93 (60.22%), Query Frame = 0

Query: 176 WQPPADGWVKVNTNASFYAASLTGGLDVLTRDSHDIVLLAATFYIPHCASVDWAEALAIK 235
           W PP   + KVN +A+F   + + GLD+L RDS   VLL+A  +I H + V  AE LA +
Sbjct: 73  WCPPLAPFFKVNVDAAFKKGNFSAGLDILIRDSTVNVLLSAMCFITHVSDVTLAECLAAQ 132

Query: 236 EGLALAIDMGVRSFLLETDSLCCFWLLDRPVDD 269
           EG+ LAI+ G+  F +ETDS   F LL    +D
Sbjct: 133 EGVCLAIEAGLIPFQIETDSSQVFNLLRTDCED 165

BLAST of Moc11g31620 vs. NCBI nr
Match: XP_022140628.1 (uncharacterized protein LOC111011237 [Momordica charantia])

HSP 1 Score: 73.9 bits (180), Expect = 2.4e-09
Identity = 41/107 (38.32%), Postives = 58/107 (54.21%), Query Frame = 0

Query: 162 SPIGSLCTRRSLYRWQPPADGWVKVNTNASFYAASLTGGLDVLTRDSHDIVLLAATFYIP 221
           +P     T  +   W PP     K+NT+ASF A+    GL ++ R+    V+ +AT Y+ 
Sbjct: 20  NPFPGRVTNTAEVLWLPPDKRIYKINTDASFLASDQHAGLGIIIRNDRGQVMASATKYLE 79

Query: 222 HCASVDWAEALAIKEGLALAIDMGVRSFLLETDSLCCFWLLDRPVDD 269
           +  SVD AEA+   EGL LA  +GV   +LETDS   F L  +P +D
Sbjct: 80  NIQSVDMAEAIVAVEGLQLASKIGVNPVILETDSSRIFNLFSQPSED 126

BLAST of Moc11g31620 vs. ExPASy Swiss-Prot
Match: P93295 (Uncharacterized mitochondrial protein AtMg00310 OS=Arabidopsis thaliana OX=3702 GN=AtMg00310 PE=4 SV=1)

HSP 1 Score: 46.2 bits (108), Expect = 6.9e-04
Identity = 21/49 (42.86%), Postives = 33/49 (67.35%), Query Frame = 0

Query: 1   MSFKNLELFNQSLLAKQDWRLLRDPTSLLARVLQGCYFRDGSFMTARLG 50
           + F++L  FNQ+LLAKQ +R++  P +LL+R+L+  YF   S M   +G
Sbjct: 57  LGFRDLGWFNQALLAKQSFRIIHQPHTLLSRLLRSRYFPHSSMMECSVG 105

BLAST of Moc11g31620 vs. ExPASy TrEMBL
Match: A0A803P8L6 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 4.6e-11
Identity = 81/303 (26.73%), Postives = 130/303 (42.90%), Query Frame = 0

Query: 1   MSFKNLELFNQSLLAKQDWRLLRDPTSLLARVLQGCYFRDGSFMTARL------------ 60
           M F+ LE FNQ+LLAKQ W++L +P  L+A+VL+  YF + +F+ A++            
Sbjct: 634 MGFRELESFNQALLAKQGWKILTNPDCLMAKVLKALYFPNNNFLEAKVGHYGSHIWRGIV 693

Query: 61  -GANLLPR------EGSLQVRSLQRLPIYTRVCDLFTASVVLVELAICSPSTLLACPVYL 120
            G  LL +         L +R +   P   R       S V V   +          ++ 
Sbjct: 694 WGRELLLKGYRWHIGNGLTIR-INEDPWLPRGAPFALRSKVNVPEGV---------TIHT 753

Query: 121 LL--ACRFAGESLLAGFLGAICLSVGSVDFAYQTIHQTCGSFGPTGKLGQDT------QH 180
           LL     +  + L   F       V  ++   +       SF P G+    +       H
Sbjct: 754 LLNHDGSWKCDELTGWFHHEDIPWVLGINPNREREDIIGWSFTPNGQYTVASGYKLRFSH 813

Query: 181 FRSVTAIHSPIGSLCTRRSLYRWQPPADGWVKVNTNASFYAASLTGGLDVLTRDSHDIVL 240
            +  T    P+ S+  ++S   W PP  G   +NT+AS        GL ++ RD    V+
Sbjct: 814 LQLQTDSTPPLPSM--KKSPVSWSPPPSGTFMINTDASLVVGQPGCGLGMVIRDHLGAVV 873

Query: 241 LAATFYIPHCASVDWAEALAIKEGLALAIDMGVRSFLLETDSLCCFWLL---DRPVDDWK 274
           +A T +IP C SV+ AE+LAI+ GL LA    + +  + +D       L   +  + DW 
Sbjct: 874 VAETIFIPGCLSVNMAESLAIRSGLKLADCWSLSNICISSDCQSVSHALNGDNHSITDWG 924

BLAST of Moc11g31620 vs. ExPASy TrEMBL
Match: A0A803PM23 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 1.8e-10
Identity = 76/274 (27.74%), Postives = 126/274 (45.99%), Query Frame = 0

Query: 1    MSFKNLELFNQSLLAKQDWRLLRDPTSLLARVLQGCYFRDGSFMTARLGA---------- 60
            M FKNLE FNQSLLAKQ W+++ +P SLLARVL+ CY+ + +F+ A++G           
Sbjct: 1031 MGFKNLEKFNQSLLAKQGWKIINNPHSLLARVLKACYYTNSNFLEAKIGGYGSFMWRSIL 1090

Query: 61   ---NLLPREGSLQVRSLQRLPIYTRVCDLFTASVVLVELAICSPSTLLACPVYLLLACRF 120
                ++ +    +V+S + + I          +  L   A  S  T L   V+      F
Sbjct: 1091 WGRQIIDKGIRWRVQSGREVRINEDKWLPRPTTFSLRTPAKVSQGTTLDDHVW-----HF 1150

Query: 121  AGESLL---AGFLGAICLSVGSVDFAYQTIHQTCGSFGPTGKLGQDTQHF--RSVTAIHS 180
                +    +G+  A+   +   + +   I +T  +     ++    ++F  R  T   +
Sbjct: 1151 TPNGIYTVNSGYRVAVTNEL-EAEASTDKITRTWWNKIWNLEIPPKVRNFIWRETTLEVN 1210

Query: 181  PIGSLCTRRSLYRWQPPADGWVKVNTNASFYAASLTGGLDVLTRDSHDIVLLAATFYIPH 240
            P       +   RW+PP  G   +N++AS     + GGL  L R+    V+ A       
Sbjct: 1211 P-------KPEKRWEPPKPGVYCLNSDASVPKNGVNGGLGALIRNHLGEVVAAEIQQQQG 1270

Query: 241  CASVDWAEALAIKEGLALAIDMGVRSFLLETDSL 257
              SV+ AE LA++ G+ LAI M    ++++TD L
Sbjct: 1271 TYSVEVAEVLALRMGIHLAIKMVAYPYIIQTDCL 1291

BLAST of Moc11g31620 vs. ExPASy TrEMBL
Match: A0A6J1DAR4 (uncharacterized protein LOC111018954 OS=Momordica charantia OX=3673 GN=LOC111018954 PE=4 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 3.9e-10
Identity = 93/420 (22.14%), Postives = 145/420 (34.52%), Query Frame = 0

Query: 1    MSFKNLELFNQSLLAKQDWRLLRDPTSLLARVLQGCYFRDGSFMTARL------------ 60
            M F++LELFN++LLAKQ WR+L  P S+L+RVL+G YF+D SFM A++            
Sbjct: 646  MGFRDLELFNKALLAKQCWRILNHPNSMLSRVLKGRYFKDCSFMEAKISGNPSYIWRSIL 705

Query: 61   -----------------------GANLLPREGSLQVRSLQRLPIYTRVCDL-------FT 120
                                   G N +P + +L++ S  RLP+ +RV  L       + 
Sbjct: 706  WGRDLLKKGLRWRIGNGDSVFIYGDNWVPNQPTLKILSSPRLPLVSRVSSLVDHEEGGWQ 765

Query: 121  ASVVLVELAICSPSTLLACP---------------------------------------- 180
              VV  E        +L+ P                                        
Sbjct: 766  GDVVRDEFTPDEAKGILSIPIGRGAEEDRLIWNYEKTGVYSVRSGYKVALLNNPCVQAPS 825

Query: 181  ------------------------------------------------------------ 240
                                                                        
Sbjct: 826  SSSSEEVRCWWNGFWKMHIPNKIKVFLWRLCLDRLPTGCNLSKRGVEITNCCYFCGRNGE 885

Query: 241  --VYLLLACRFAGESLLAGFLGAIC---------LSVGSVDFAYQTI-------HQTCGS 251
              ++L   C+FA    +    G +           S+   DF    +        +   +
Sbjct: 886  DSIHLFWICKFAEALWINSKFGKLSPFLILRESHESLSKADFEELCVVIWGLWNQRNARA 945

BLAST of Moc11g31620 vs. ExPASy TrEMBL
Match: A0A803NUL5 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 3.9e-10
Identity = 73/263 (27.76%), Postives = 113/263 (42.97%), Query Frame = 0

Query: 1   MSFKNLELFNQSLLAKQDWRLLRDPTSLLARVLQGCYFRDGSFMTARLGANLLPREGSLQ 60
           M F++LE FNQ+LLAKQ W++L+ P  LLARVL+  YF + +F  A+LG       GS  
Sbjct: 185 MGFRDLEDFNQALLAKQGWKILQQPDCLLARVLKALYFPNENFFEAKLG-----HFGS-- 244

Query: 61  VRSLQRLPIYTRVCDLFTASVVLVELAICSPSTLLACPVYLLLACRFAGESLLAGFLG-A 120
             S+ R  ++ R  DL   +                            G+  +AGF+G +
Sbjct: 245 --SVWRGILWGR--DLIVKAY---------------------------GKGRVAGFIGFS 304

Query: 121 ICLSVGSVDFAYQTIHQTCGSFGPTG------KLGQDTQHFRSVTAIHSPIGSLCTRRSL 180
             +     +F +Q  H     +          +LGQ  Q           +  L   +  
Sbjct: 305 WLIWQRRNNFVFQQKHPADHLWLSWAVDFIAYQLGQQQQ-----------LPLLVHNKPT 364

Query: 181 YRWQPPADGWVKVNTNASFYAASLTGGLDVLTRDSHDIVLLAATFYIPHCASVDWAEALA 240
             WQPP   +  +NT+AS     L  GL  + R+    +++A   YI  C +V  AEA A
Sbjct: 365 VSWQPPPSDFHLINTDASLKLGHLGCGLSAIIRNPAGDLVVAEVKYIQGCMTVPMAEAAA 398

Query: 241 IKEGLALAIDMGVRSFLLETDSL 257
           I  G+ LA+   +    + +D +
Sbjct: 425 IHLGVQLAVRWSINKAWVGSDCI 398

BLAST of Moc11g31620 vs. ExPASy TrEMBL
Match: A0A6A3B8N2 (RNase H domain-containing protein OS=Hibiscus syriacus OX=106335 GN=F3Y22_tig00110279pilonHSYRG00089 PE=4 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 5.1e-10
Identity = 78/268 (29.10%), Postives = 119/268 (44.40%), Query Frame = 0

Query: 1   MSFKNLELFNQSLLAKQDWRLLRDPTSLLARVLQGCYFRDGSFMTARLGANLLPREGSLQ 60
           +  ++LE+FNQ+ L KQ WRL     +L+A+VL+  Y+ DGSF+TA L   ++    S++
Sbjct: 82  LGIRDLEVFNQAFLCKQGWRLFNGSKTLVAQVLKAKYYPDGSFLTADLEVLIIFFYVSMR 141

Query: 61  VRSLQRLPIYTRVCDLFTASVVLVELAICSPSTLLACPVYLLL-ACRFAGESLLA----- 120
              LQ    +  + + F  +    ELA       L C + L+  +C   G  L+      
Sbjct: 142 NERLQMPGTFISI-ECFGHNRPREELA-----KSLFCVLSLVAPSCTRRGGCLVGSSSPR 201

Query: 121 ---GFLGAIC-LSVGSVDF-----AYQTIHQTCGSFGPTGKLGQDTQHFRSVTAIHSPIG 180
              G  G +C LS+    F      +   + + GSF   G  G                 
Sbjct: 202 VTKGTSGNVCFLSLPGPYFLGCGDPWVCDYLSRGSFFGVGSPG----------------- 261

Query: 181 SLCTRRSLYR-WQPPADGWVKVNTNASFYAASLTGGLDVLTRDSHDIVLLAATFYIPHCA 240
             C    + R W  P  G++KVN + S+     TGG+  + RDS    L   +  IPH  
Sbjct: 262 --CGSYPIVRLWTIPPAGFIKVNVDGSYDKDLRTGGICCVLRDSTGQFLAGCSSGIPHAM 321

Query: 241 SVDWAEALAIKEGLALAIDMGVRSFLLE 253
                E+LA  EGL LA+ +G RS ++E
Sbjct: 322 DAFPVESLACLEGLNLALSLGCRSLVVE 324

BLAST of Moc11g31620 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 47.4 bits (111), Expect = 2.2e-05
Identity = 22/50 (44.00%), Postives = 32/50 (64.00%), Query Frame = 0

Query: 1   MSFKNLELFNQSLLAKQDWRLLRDPTSLLARVLQGCYFRDGSFMTARLGA 51
           + FK++E FN +LL KQ WR+L  P SL+A+V +  YF     + A LG+
Sbjct: 56  IGFKDIEAFNLALLGKQMWRMLSRPESLMAKVFKSRYFHKSDPLNAPLGS 105

BLAST of Moc11g31620 vs. TAIR 10
Match: ATMG00310.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 46.2 bits (108), Expect = 4.9e-05
Identity = 21/49 (42.86%), Postives = 33/49 (67.35%), Query Frame = 0

Query: 1   MSFKNLELFNQSLLAKQDWRLLRDPTSLLARVLQGCYFRDGSFMTARLG 50
           + F++L  FNQ+LLAKQ +R++  P +LL+R+L+  YF   S M   +G
Sbjct: 57  LGFRDLGWFNQALLAKQSFRIIHQPHTLLSRLLRSRYFPHSSMMECSVG 105

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_024196130.15.1e-1226.47uncharacterized protein LOC112199331 [Rosa chinensis][more]
XP_022150918.18.1e-1022.14uncharacterized protein LOC111018954 [Momordica charantia][more]
KAE8711725.11.1e-0929.10hypothetical protein F3Y22_tig00110279pilonHSYRG00089 [Hibiscus syriacus][more]
XP_022135942.11.8e-0944.09uncharacterized protein LOC111007775 [Momordica charantia][more]
XP_022140628.12.4e-0938.32uncharacterized protein LOC111011237 [Momordica charantia][more]
Match NameE-valueIdentityDescription
P932956.9e-0442.86Uncharacterized mitochondrial protein AtMg00310 OS=Arabidopsis thaliana OX=3702 ... [more]
Match NameE-valueIdentityDescription
A0A803P8L64.6e-1126.73Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A803PM231.8e-1027.74Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A6J1DAR43.9e-1022.14uncharacterized protein LOC111018954 OS=Momordica charantia OX=3673 GN=LOC111018... [more]
A0A803NUL53.9e-1027.76Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A6A3B8N25.1e-1029.10RNase H domain-containing protein OS=Hibiscus syriacus OX=106335 GN=F3Y22_tig001... [more]
Match NameE-valueIdentityDescription
AT4G29090.12.2e-0544.00Ribonuclease H-like superfamily protein [more]
ATMG00310.14.9e-0542.86RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 187..259
e-value: 7.3E-9
score: 35.5
NoneNo IPR availablePANTHERPTHR47074BNAC02G40300D PROTEINcoord: 171..270
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 182..270

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc11g31620.1Moc11g31620.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity