Moc11g31580 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc11g31580
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptionprotein NRT1/ PTR FAMILY 5.10-like
Locationchr11: 23035185 .. 23036486 (-)
RNA-Seq ExpressionMoc11g31580
SyntenyMoc11g31580
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAGTCCTCTGTTGGATGACAGTGAGACGGTGGACGGAGCAGTTGATTACAGTGGCCGGCCAGCCCACAGATCCAGTTCCGGCGGCTGGAGATCCGCCTCATTCATCATAGGTAAATCAAATCAGAACCCACAACACAATTTCTTGCTTTAATTCGCACTCTCATTATCATTATCATTATCATTAGGAGTGGAGGTTGTGGAGAGATTTGCCTACTACGGAGTCAGCTCCAACTTGATTAACTTCTTGACGGACCAGCTCCGGCAGTCGACGGCCACCGCAGCGGAGAATGTCAACGCCTTGTCCGGTGCAGCCTCGCTGCTCCCTCTTCTGGGGGCCCTTGTCGCCGACTCCTTCCTCGGCCGATATCCCACCATCCTTCTTTCCTCTGCCCTCTACATCTTGGTTGCTCTTTCTCCTCTCCTCTCTTCCCAAAATATGCTTCAACTGACCCTCTTTAACCCTTTTTCTTCTTCGAATTCAGGCTCTTGGGCTGCTCACTGTGTCTGCAACATTTCCTTCTCCCACCATCTCTGCTTCCCTACACACTGAACAAGCTTCCCCCAGTGCAGCCCAACAAATTTTATTTTTCTCCTCCTTGTATCTGATGGCAGTTGCTCAAGGTGGACACAGGCCTTGCGTCCAGGCTTTCGGAGCCGATCAATTTGATGCCCAACATCCACAAGAAACCCAGGCCAAAAGCTCTTTCTTCAATTGGTGGTACTTTTGCATCTCCCTGGCCACTTTTGTAACTGTTAACATCTTGAACTATGTGCAAGACAACCTTAGTTGGGTTCTTGGGTTTGGAATTCCTTGTATTGCCATGCTTTTTGCGTTGCTGGTTTTCTTGCTTGGAACTAGGACGTACAGGTACAGCAACAGAGGAGCCGAGGAAAACCCATTTGTAAGGATTGGACGGGTGTTTATCACAGCACTGAGAAATTGGCATCTGAGTCCTTCAGAGATGGCTCATGAACAGGAAACCCCTGTCCTTTTGGCTCACCGTACTTCTCAACAATTCAGGTAAATCATACGACAAAACAAGGCTAAAATGTGCGTCTCTTTACACCTATTATTGGTTTTGGATTCTCTAATGTGGCAGGTTTCTGGACAAAGCTTTAGTTGTTCCTCATGGTTCAAAGGAAGACAGTCTCGTATGTAGTGTCAGTGAAATTGAGGAAGCGAAAGCAGTACTTAGGCTTGGTCCCATATGGGTTACATGCTTAGCATATGCTCTTGTGTTCTCTCAGGCATTCACTTTCTTTACAAAACAAGGAGTCACAATGGACAGATCAGTAG

mRNA sequence

ATGGAGAGTCCTCTGTTGGATGACAGTGAGACGGTGGACGGAGCAGTTGATTACAGTGGCCGGCCAGCCCACAGATCCAGTTCCGGCGGCTGGAGATCCGCCTCATTCATCATAGGAGTGGAGGTTGTGGAGAGATTTGCCTACTACGGAGTCAGCTCCAACTTGATTAACTTCTTGACGGACCAGCTCCGGCAGTCGACGGCCACCGCAGCGGAGAATGTCAACGCCTTGTCCGGTGCAGCCTCGCTGCTCCCTCTTCTGGGGGCCCTTGTCGCCGACTCCTTCCTCGGCCGATATCCCACCATCCTTCTTTCCTCTGCCCTCTACATCTTGGCTCTTGGGCTGCTCACTGTGTCTGCAACATTTCCTTCTCCCACCATCTCTGCTTCCCTACACACTGAACAAGCTTCCCCCAGTGCAGCCCAACAAATTTTATTTTTCTCCTCCTTGTATCTGATGGCAGTTGCTCAAGGTGGACACAGGCCTTGCGTCCAGGCTTTCGGAGCCGATCAATTTGATGCCCAACATCCACAAGAAACCCAGGCCAAAAGCTCTTTCTTCAATTGGTGGTACTTTTGCATCTCCCTGGCCACTTTTGTAACTGTTAACATCTTGAACTATGTGCAAGACAACCTTAGTTGGGTTCTTGGGTTTGGAATTCCTTGTATTGCCATGCTTTTTGCGTTGCTGGTTTTCTTGCTTGGAACTAGGACGTACAGGTACAGCAACAGAGGAGCCGAGGAAAACCCATTTGTAAGGATTGGACGGGTGTTTATCACAGCACTGAGAAATTGGCATCTGAGTCCTTCAGAGATGGCTCATGAACAGGAAACCCCTGTCCTTTTGGCTCACCGTACTTCTCAACAATTCAGGCATTCACTTTCTTTACAAAACAAGGAGTCACAATGGACAGATCAGTAG

Coding sequence (CDS)

ATGGAGAGTCCTCTGTTGGATGACAGTGAGACGGTGGACGGAGCAGTTGATTACAGTGGCCGGCCAGCCCACAGATCCAGTTCCGGCGGCTGGAGATCCGCCTCATTCATCATAGGAGTGGAGGTTGTGGAGAGATTTGCCTACTACGGAGTCAGCTCCAACTTGATTAACTTCTTGACGGACCAGCTCCGGCAGTCGACGGCCACCGCAGCGGAGAATGTCAACGCCTTGTCCGGTGCAGCCTCGCTGCTCCCTCTTCTGGGGGCCCTTGTCGCCGACTCCTTCCTCGGCCGATATCCCACCATCCTTCTTTCCTCTGCCCTCTACATCTTGGCTCTTGGGCTGCTCACTGTGTCTGCAACATTTCCTTCTCCCACCATCTCTGCTTCCCTACACACTGAACAAGCTTCCCCCAGTGCAGCCCAACAAATTTTATTTTTCTCCTCCTTGTATCTGATGGCAGTTGCTCAAGGTGGACACAGGCCTTGCGTCCAGGCTTTCGGAGCCGATCAATTTGATGCCCAACATCCACAAGAAACCCAGGCCAAAAGCTCTTTCTTCAATTGGTGGTACTTTTGCATCTCCCTGGCCACTTTTGTAACTGTTAACATCTTGAACTATGTGCAAGACAACCTTAGTTGGGTTCTTGGGTTTGGAATTCCTTGTATTGCCATGCTTTTTGCGTTGCTGGTTTTCTTGCTTGGAACTAGGACGTACAGGTACAGCAACAGAGGAGCCGAGGAAAACCCATTTGTAAGGATTGGACGGGTGTTTATCACAGCACTGAGAAATTGGCATCTGAGTCCTTCAGAGATGGCTCATGAACAGGAAACCCCTGTCCTTTTGGCTCACCGTACTTCTCAACAATTCAGGCATTCACTTTCTTTACAAAACAAGGAGTCACAATGGACAGATCAGTAG

Protein sequence

MESPLLDDSETVDGAVDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLTDQLRQSTATAAENVNALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSATFPSPTISASLHTEQASPSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHPQETQAKSSFFNWWYFCISLATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTRTYRYSNRGAEENPFVRIGRVFITALRNWHLSPSEMAHEQETPVLLAHRTSQQFRHSLSLQNKESQWTDQ
Homology
BLAST of Moc11g31580 vs. NCBI nr
Match: XP_022155214.1 (protein NRT1/ PTR FAMILY 5.10-like [Momordica charantia])

HSP 1 Score: 567.4 bits (1461), Expect = 7.5e-158
Identity = 291/291 (100.00%), Postives = 291/291 (100.00%), Query Frame = 0

Query: 1   MESPLLDDSETVDGAVDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLT 60
           MESPLLDDSETVDGAVDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLT
Sbjct: 1   MESPLLDDSETVDGAVDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLT 60

Query: 61  DQLRQSTATAAENVNALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSA 120
           DQLRQSTATAAENVNALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSA
Sbjct: 61  DQLRQSTATAAENVNALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSA 120

Query: 121 TFPSPTISASLHTEQASPSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHPQET 180
           TFPSPTISASLHTEQASPSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHPQET
Sbjct: 121 TFPSPTISASLHTEQASPSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHPQET 180

Query: 181 QAKSSFFNWWYFCISLATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTRTYR 240
           QAKSSFFNWWYFCISLATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTRTYR
Sbjct: 181 QAKSSFFNWWYFCISLATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTRTYR 240

Query: 241 YSNRGAEENPFVRIGRVFITALRNWHLSPSEMAHEQETPVLLAHRTSQQFR 292
           YSNRGAEENPFVRIGRVFITALRNWHLSPSEMAHEQETPVLLAHRTSQQFR
Sbjct: 241 YSNRGAEENPFVRIGRVFITALRNWHLSPSEMAHEQETPVLLAHRTSQQFR 291

BLAST of Moc11g31580 vs. NCBI nr
Match: XP_022155213.1 (protein NRT1/ PTR FAMILY 5.10-like [Momordica charantia])

HSP 1 Score: 450.3 bits (1157), Expect = 1.3e-122
Identity = 237/293 (80.89%), Postives = 256/293 (87.37%), Query Frame = 0

Query: 4   PLLDDSETVDGAVDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLTDQL 63
           PLLD  ETV+GAVDY GRP HRS+SGGWRSA+ IIGVEV ERFAYYGVSSNLINFLTDQL
Sbjct: 6   PLLD--ETVEGAVDYQGRPVHRSNSGGWRSAALIIGVEVAERFAYYGVSSNLINFLTDQL 65

Query: 64  RQSTATAAENVNALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSATFP 123
            QSTA AA NVNA SGAASLLPLLGA +ADSFLGR+ TILLSSALY+L LGLLT+SATFP
Sbjct: 66  GQSTAMAAANVNAWSGAASLLPLLGAFLADSFLGRFRTILLSSALYVLGLGLLTLSATFP 125

Query: 124 SPTISA--SLHTEQA---SPSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHPQ 183
           SP ISA    HT++A   SPS AQ ILFFSSLYL+AVAQGGH+PCVQAFGADQFD QHP+
Sbjct: 126 SPNISACQGQHTQKAVPCSPSDAQVILFFSSLYLVAVAQGGHKPCVQAFGADQFDGQHPE 185

Query: 184 ETQAKSSFFNWWYFCISLATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTRT 243
           ET+AKSSFFNWWYFCISLATF TVNILNYVQDNLSWVLGFGIP IAM+ ALLVFLLGTR 
Sbjct: 186 ETKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWVLGFGIPFIAMVLALLVFLLGTRR 245

Query: 244 YRYSNRGAEENPFVRIGRVFITALRNWHLSPSEMAHEQETPVLLAHRTSQQFR 292
           YRYSN G EENPFVRIGRVFITALRNW L+ SE+AHE+E   LL H +S+QFR
Sbjct: 246 YRYSNTGDEENPFVRIGRVFITALRNWRLTSSEIAHEEEIRGLLPHHSSRQFR 296

BLAST of Moc11g31580 vs. NCBI nr
Match: XP_023535809.1 (protein NRT1/ PTR FAMILY 5.10-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023535810.1 protein NRT1/ PTR FAMILY 5.10-like isoform X2 [Cucurbita pepo subsp. pepo] >XP_023535814.1 protein NRT1/ PTR FAMILY 5.10-like isoform X6 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 439.9 bits (1130), Expect = 1.8e-119
Identity = 227/294 (77.21%), Postives = 255/294 (86.73%), Query Frame = 0

Query: 1   MESPLLDDSETVDGAVDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLT 60
           ME+PLL+D  TV+GAVDY+GRP  RS SGGWRSA+ IIGVEV ERFAYYGVSSNLI+FLT
Sbjct: 1   MEAPLLED--TVEGAVDYNGRPVRRSDSGGWRSAALIIGVEVAERFAYYGVSSNLISFLT 60

Query: 61  DQLRQSTATAAENVNALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSA 120
           DQL QSTATAAENVNA SGAASLLPLLGA +ADSFLGRY TI LS+ LYIL LGLLTVSA
Sbjct: 61  DQLGQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSTVLYILGLGLLTVSA 120

Query: 121 TFPSPTISASLHTEQA---SPSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHP 180
           T  SP+IS+   TE++   SP   Q ILFFSSLY++A AQGGH+PCVQAFGADQFD QHP
Sbjct: 121 TLHSPSISSCQQTEKSLPCSPGLFQVILFFSSLYVVAFAQGGHKPCVQAFGADQFDGQHP 180

Query: 181 QETQAKSSFFNWWYFCISLATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTR 240
           +E++AKSSFFNWWYFCISLATF TVNILNYVQDNLSW LGFGIPCI+M+ AL+VFLLGTR
Sbjct: 181 EESKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWSLGFGIPCISMVLALVVFLLGTR 240

Query: 241 TYRYSNRGAEENPFVRIGRVFITALRNWHLSPSEMAHEQETPVLLAHRTSQQFR 292
           TYR+ NRG EENPFVRIGRVFITA+RNW ++ SE+AHE+ET  LL H +SQQFR
Sbjct: 241 TYRFINRGDEENPFVRIGRVFITAVRNWRVNSSEIAHEEETRGLLPHHSSQQFR 292

BLAST of Moc11g31580 vs. NCBI nr
Match: XP_023535815.1 (protein NRT1/ PTR FAMILY 5.10-like isoform X7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 438.7 bits (1127), Expect = 4.0e-119
Identity = 227/294 (77.21%), Postives = 252/294 (85.71%), Query Frame = 0

Query: 1   MESPLLDDSETVDGAVDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLT 60
           ME+PLLD  ETV+GAVDY+GRP  RS  GGWRSASFIIGVEV ERFAYYGVSSNLINFLT
Sbjct: 1   MEAPLLD--ETVEGAVDYNGRPVLRSKVGGWRSASFIIGVEVAERFAYYGVSSNLINFLT 60

Query: 61  DQLRQSTATAAENVNALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSA 120
           DQL QSTATAAENVN  SG ASLLPLLGA +ADSF+GRY TI LSS LYIL LGLLTVSA
Sbjct: 61  DQLGQSTATAAENVNVWSGVASLLPLLGAFLADSFIGRYRTIALSSVLYILGLGLLTVSA 120

Query: 121 TFPSPTISASLHTEQA---SPSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHP 180
           T  SP+IS+   TE++   SP   Q ILFFSSLY++A AQGGH+PCVQAFGADQFD QHP
Sbjct: 121 TLHSPSISSCQQTEKSLPCSPGLFQVILFFSSLYVVAFAQGGHKPCVQAFGADQFDGQHP 180

Query: 181 QETQAKSSFFNWWYFCISLATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTR 240
           +E++AKSSFFNWWYFCISLATF TVNILNYVQDNLSW LGFGIPCI+M+ AL+VFLLGTR
Sbjct: 181 EESKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWSLGFGIPCISMVLALVVFLLGTR 240

Query: 241 TYRYSNRGAEENPFVRIGRVFITALRNWHLSPSEMAHEQETPVLLAHRTSQQFR 292
           TYR+ NRG EENPFVRIGRVFITA+RNW ++ SE+AHE+E   LL H +SQQFR
Sbjct: 241 TYRFINRGDEENPFVRIGRVFITAVRNWRINSSEIAHEEEIRGLLPHHSSQQFR 292

BLAST of Moc11g31580 vs. NCBI nr
Match: KAG6592396.1 (Protein NRT1/ PTR FAMILY 5.10, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 438.3 bits (1126), Expect = 5.3e-119
Identity = 227/294 (77.21%), Postives = 252/294 (85.71%), Query Frame = 0

Query: 1   MESPLLDDSETVDGAVDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLT 60
           ME+PLL+D  TV+GAVDY GRP  RS SGGWRSA+ IIGVEV ERFAYYGVSSNLINFLT
Sbjct: 1   MEAPLLED--TVEGAVDYHGRPVRRSDSGGWRSAALIIGVEVAERFAYYGVSSNLINFLT 60

Query: 61  DQLRQSTATAAENVNALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSA 120
           DQL QSTATAAENVN  SGAASLLPLLGA +ADSFLGRY TI LSS LYIL LGLLTVSA
Sbjct: 61  DQLGQSTATAAENVNVWSGAASLLPLLGAFLADSFLGRYRTIALSSVLYILGLGLLTVSA 120

Query: 121 TFPSPTISASLHTEQA---SPSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHP 180
           T  SP+IS    TE++   SP   Q ILFFSS+Y++A AQGGH+PCVQAFGADQFDAQHP
Sbjct: 121 TLHSPSISGCQQTEKSLPCSPGLFQVILFFSSVYIVAFAQGGHKPCVQAFGADQFDAQHP 180

Query: 181 QETQAKSSFFNWWYFCISLATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTR 240
           +E++AKSSFFNWWYFCISLATF TVNILNYVQDNLSW LGFGIPCI+M+ AL+VFLLGTR
Sbjct: 181 EESKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWSLGFGIPCISMVLALVVFLLGTR 240

Query: 241 TYRYSNRGAEENPFVRIGRVFITALRNWHLSPSEMAHEQETPVLLAHRTSQQFR 292
           TYR+ NRG EENPFVRIGRVFITA+RNW ++ SE+AHE+E   LL H +SQQFR
Sbjct: 241 TYRFINRGDEENPFVRIGRVFITAVRNWRVNSSEIAHEEEICGLLPHHSSQQFR 292

BLAST of Moc11g31580 vs. ExPASy Swiss-Prot
Match: Q0WP01 (Protein NRT1/ PTR FAMILY 5.10 OS=Arabidopsis thaliana OX=3702 GN=NPF5.10 PE=2 SV=1)

HSP 1 Score: 331.3 bits (848), Expect = 1.2e-89
Identity = 174/275 (63.27%), Postives = 213/275 (77.45%), Query Frame = 0

Query: 16  VDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLTDQLRQSTATAAENVN 75
           VDY  +PA +SSSGGWRSA FIIGVEV ERFAYYG+SSNLI +LT  L QSTA AA NVN
Sbjct: 19  VDYRNKPAVKSSSGGWRSAGFIIGVEVAERFAYYGISSNLITYLTGPLGQSTAAAAANVN 78

Query: 76  ALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSATFPSPTISASLHTEQ 135
           A SG ASLLPLLGA VADSFLGR+ TIL +SALYI+ LG+LT+SA  PS    ++L    
Sbjct: 79  AWSGTASLLPLLGAFVADSFLGRFRTILAASALYIVGLGVLTLSAMIPSDCKVSNL-LSS 138

Query: 136 ASPSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHPQETQAKSSFFNWWYFCIS 195
            SP   Q I FFS+LYL+A+AQGGH+PCVQAFGADQFD + P+E +AKSSFFNWWYF + 
Sbjct: 139 CSP-RFQVITFFSALYLVALAQGGHKPCVQAFGADQFDEKEPEECKAKSSFFNWWYFGMC 198

Query: 196 LATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTRTYRYSNRGAEENPFVRIG 255
             T  T+ +LNY+QDNLSW LGFGIPCIAM+ AL+V LLGT TYR+S R  +++PFVRIG
Sbjct: 199 FGTLTTLWVLNYIQDNLSWALGFGIPCIAMVVALVVLLLGTCTYRFSIRREDQSPFVRIG 258

Query: 256 RVFITALRNWHLSPSEMAHEQETPVLLAHRTSQQF 291
            V++ A++NW +S  ++A  +E   L++  +SQQF
Sbjct: 259 NVYVAAVKNWSVSALDVAAAEERLGLVSCSSSQQF 291

BLAST of Moc11g31580 vs. ExPASy Swiss-Prot
Match: Q9SK99 (Protein NRT1/ PTR FAMILY 5.15 OS=Arabidopsis thaliana OX=3702 GN=NPF5.15 PE=2 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 1.1e-74
Identity = 147/267 (55.06%), Postives = 190/267 (71.16%), Query Frame = 0

Query: 2   ESPLLDDSETVDGAVDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLTD 61
           E  LL+D   V  +VD+ G PA +SS+GGWRSA FIIGVEV ERFAY+G++ NLI +LT 
Sbjct: 7   EVALLED--YVSDSVDHRGFPAGKSSTGGWRSAWFIIGVEVAERFAYFGIACNLITYLTG 66

Query: 62  QLRQSTATAAENVNALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSAT 121
            L QSTA AA NVN  SG AS+LP+LGA VAD++LGRY TI+++S +YIL LGLLT+SA+
Sbjct: 67  PLGQSTAKAAVNVNTWSGTASILPILGAFVADAYLGRYRTIVVASLIYILGLGLLTLSAS 126

Query: 122 FPSPTISASLHTEQASPSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHPQETQ 181
                +S   +   A PS     LFF SLYL+A+ QGGH+PCVQAFGADQFDA+ P+E  
Sbjct: 127 LIIMGLSKQRNDASAKPSIWVNTLFFCSLYLVAIGQGGHKPCVQAFGADQFDAEDPKEVI 186

Query: 182 AKSSFFNWWYFCISLATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTRTYRY 241
           A+ SFFNWW+  +S    +++ ++ YVQ+N++W  GFGIPC+ M+ AL +FLLG + YRY
Sbjct: 187 ARGSFFNWWFLSLSAGISISIIVVAYVQENVNWAFGFGIPCLFMVMALAIFLLGRKIYRY 246

Query: 242 SNRGAEE----NPFVRIGRVFITALRN 265
                EE    N F RIGRVF+ A +N
Sbjct: 247 PKGHHEEVNSSNTFARIGRVFVIAFKN 271

BLAST of Moc11g31580 vs. ExPASy Swiss-Prot
Match: Q9SK96 (Protein NRT1/ PTR FAMILY 5.16 OS=Arabidopsis thaliana OX=3702 GN=NPF5.16 PE=2 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 2.7e-73
Identity = 147/268 (54.85%), Postives = 193/268 (72.01%), Query Frame = 0

Query: 2   ESPLLDDSETVDGAVDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLTD 61
           E+ L++DS  V  +VD+ G PA +SS+GGWRSA +IIGVEV ERFAY+G+ SNLI +LT 
Sbjct: 7   EAALIEDS--VSDSVDHRGLPAGKSSTGGWRSAWYIIGVEVGERFAYFGIGSNLITYLTG 66

Query: 62  QLRQSTATAAENVNALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSAT 121
            L QSTATAA NVN  SG AS+LP+LGA +AD++LGRY TI+++S +YIL LGLLT+S+ 
Sbjct: 67  PLGQSTATAAVNVNTWSGTASILPVLGAFIADAYLGRYRTIVVASLIYILGLGLLTLSSI 126

Query: 122 FPSPTISAS-LHTEQAS--PSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHPQ 181
                +S    H   AS  P     ILFF SLYL+A+ QGGH+PCVQAFGADQFD   P+
Sbjct: 127 LILMGLSEQRQHNRNASAKPFFWVNILFFCSLYLVAIGQGGHKPCVQAFGADQFDVGDPK 186

Query: 182 ETQAKSSFFNWWYFCISLATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTRT 241
           E  ++ SFFNWW+  +S    +++ ++ YVQDN++W LGFGIPC+ M+ AL +FL G +T
Sbjct: 187 ERISRGSFFNWWFLSLSAGITLSIIVVVYVQDNVNWALGFGIPCLFMVMALALFLFGRKT 246

Query: 242 YRY--SNRGAEENPFVRIGRVFITALRN 265
           YRY   +R  + N F RIGRVF+ A +N
Sbjct: 247 YRYPRGDREGKNNAFARIGRVFLVAFKN 272

BLAST of Moc11g31580 vs. ExPASy Swiss-Prot
Match: Q9C7U1 (Protein NRT1/ PTR FAMILY 5.12 OS=Arabidopsis thaliana OX=3702 GN=NPF5.12 PE=2 SV=1)

HSP 1 Score: 276.6 bits (706), Expect = 3.5e-73
Identity = 145/296 (48.99%), Postives = 204/296 (68.92%), Query Frame = 0

Query: 2   ESPLLDDSETVDGAVDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLTD 61
           E+  +  +E V+ +VD+ G P+ RSSSG W+S+ F +  EV E+FAY+G++SNLI + T+
Sbjct: 9   ETGAIVSNENVEFSVDFRGNPSIRSSSGAWKSSGFTMCAEVAEKFAYFGIASNLITYFTE 68

Query: 62  QLRQSTATAAENVNALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSAT 121
            L +STA AA NVN   G A+ LPL+   +ADSFLGR+ TILL+S+ YI+ LGLLT SAT
Sbjct: 69  ALGESTAVAASNVNLWLGTAAFLPLIWGSIADSFLGRFRTILLTSSFYIMGLGLLTFSAT 128

Query: 122 FPSPTISASLHTEQASPSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHPQETQ 181
            PS  +     T ++  S  + I+FF +LYL+A+ +GG + C++AFGADQFD Q P E++
Sbjct: 129 IPS--LCNDQETRESCVSQVKVIIFFCALYLIALGEGGFKVCLRAFGADQFDEQDPNESK 188

Query: 182 AKSSFFNWWYFCISLATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTRTYRY 241
           AKSS+FNW YF IS+    T  + NYVQ+NLSW LG+ IPC++M+ AL +FLLG +TYR+
Sbjct: 189 AKSSYFNWLYFAISIGILTTRLVTNYVQENLSWALGYAIPCLSMMLALFLFLLGIKTYRF 248

Query: 242 SNRG------AEENPFVRIGRVFITALRNWHLSPSEMAHEQETPVLLAHRTSQQFR 292
           S  G        +NPFVRIGRVF+ A RN   +PS      +T +LL + ++++FR
Sbjct: 249 STGGEGRQGKKHDNPFVRIGRVFVAAARNRRQTPS------DTCLLLPNESTKKFR 296

BLAST of Moc11g31580 vs. ExPASy Swiss-Prot
Match: Q8VZE2 (Protein NRT1/ PTR FAMILY 5.14 OS=Arabidopsis thaliana OX=3702 GN=NPF5.14 PE=2 SV=2)

HSP 1 Score: 275.4 bits (703), Expect = 7.8e-73
Identity = 151/295 (51.19%), Postives = 195/295 (66.10%), Query Frame = 0

Query: 10  ETVDGAVDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLTDQLRQSTAT 69
           E V  AVD+ G  A RS++G WR+A FIIGVEV ERFAYYG+ SNLI++LT  L +STA 
Sbjct: 12  EYVTDAVDHRGLAARRSNTGRWRAALFIIGVEVAERFAYYGIGSNLISYLTGPLGESTAV 71

Query: 70  AAENVNALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSATFPSPTISA 129
           AA NVNA SG A+LLP+LGA VAD+FLGRY TI++SS +Y+L L  LT+SA     T   
Sbjct: 72  AAANVNAWSGIATLLPVLGAFVADAFLGRYRTIIISSLIYVLGLAFLTLSAFLIPNTTEV 131

Query: 130 SLHTEQASPSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHPQETQAKSSFFNW 189
           +     +S S+   +LFF SLYL+A+ Q GH+PCVQAFGADQFD +  QE   +SSFFNW
Sbjct: 132 T-----SSTSSFLNVLFFFSLYLVAIGQSGHKPCVQAFGADQFDEKDSQEKSDRSSFFNW 191

Query: 190 WYFCISLATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTRTYRYSNRGAEE- 249
           WY  +S      + ++ Y+Q+  SW  GFGIPC+ M+ +L++F+ G R YRYS R  EE 
Sbjct: 192 WYLSLSAGICFAILVVVYIQEEFSWAFGFGIPCVFMVISLVLFVSGRRIYRYSKRRHEEE 251

Query: 250 -NPFVRIGRVFITALRNWHLSPSEMAHEQETPVLLAHRTSQQFRHSLSLQNKESQ 303
            NPF RIGRVF  AL+N  LS S++  + E     +      F  +L + N  SQ
Sbjct: 252 INPFTRIGRVFFVALKNQRLSSSDLC-KVELEANTSPEKQSFFNKALLVPNDSSQ 300

BLAST of Moc11g31580 vs. ExPASy TrEMBL
Match: A0A6J1DPL2 (protein NRT1/ PTR FAMILY 5.10-like OS=Momordica charantia OX=3673 GN=LOC111022356 PE=3 SV=1)

HSP 1 Score: 567.4 bits (1461), Expect = 3.7e-158
Identity = 291/291 (100.00%), Postives = 291/291 (100.00%), Query Frame = 0

Query: 1   MESPLLDDSETVDGAVDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLT 60
           MESPLLDDSETVDGAVDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLT
Sbjct: 1   MESPLLDDSETVDGAVDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLT 60

Query: 61  DQLRQSTATAAENVNALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSA 120
           DQLRQSTATAAENVNALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSA
Sbjct: 61  DQLRQSTATAAENVNALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSA 120

Query: 121 TFPSPTISASLHTEQASPSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHPQET 180
           TFPSPTISASLHTEQASPSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHPQET
Sbjct: 121 TFPSPTISASLHTEQASPSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHPQET 180

Query: 181 QAKSSFFNWWYFCISLATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTRTYR 240
           QAKSSFFNWWYFCISLATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTRTYR
Sbjct: 181 QAKSSFFNWWYFCISLATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTRTYR 240

Query: 241 YSNRGAEENPFVRIGRVFITALRNWHLSPSEMAHEQETPVLLAHRTSQQFR 292
           YSNRGAEENPFVRIGRVFITALRNWHLSPSEMAHEQETPVLLAHRTSQQFR
Sbjct: 241 YSNRGAEENPFVRIGRVFITALRNWHLSPSEMAHEQETPVLLAHRTSQQFR 291

BLAST of Moc11g31580 vs. ExPASy TrEMBL
Match: A0A6J1DMD5 (protein NRT1/ PTR FAMILY 5.10-like OS=Momordica charantia OX=3673 GN=LOC111022355 PE=3 SV=1)

HSP 1 Score: 450.3 bits (1157), Expect = 6.5e-123
Identity = 237/293 (80.89%), Postives = 256/293 (87.37%), Query Frame = 0

Query: 4   PLLDDSETVDGAVDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLTDQL 63
           PLLD  ETV+GAVDY GRP HRS+SGGWRSA+ IIGVEV ERFAYYGVSSNLINFLTDQL
Sbjct: 6   PLLD--ETVEGAVDYQGRPVHRSNSGGWRSAALIIGVEVAERFAYYGVSSNLINFLTDQL 65

Query: 64  RQSTATAAENVNALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSATFP 123
            QSTA AA NVNA SGAASLLPLLGA +ADSFLGR+ TILLSSALY+L LGLLT+SATFP
Sbjct: 66  GQSTAMAAANVNAWSGAASLLPLLGAFLADSFLGRFRTILLSSALYVLGLGLLTLSATFP 125

Query: 124 SPTISA--SLHTEQA---SPSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHPQ 183
           SP ISA    HT++A   SPS AQ ILFFSSLYL+AVAQGGH+PCVQAFGADQFD QHP+
Sbjct: 126 SPNISACQGQHTQKAVPCSPSDAQVILFFSSLYLVAVAQGGHKPCVQAFGADQFDGQHPE 185

Query: 184 ETQAKSSFFNWWYFCISLATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTRT 243
           ET+AKSSFFNWWYFCISLATF TVNILNYVQDNLSWVLGFGIP IAM+ ALLVFLLGTR 
Sbjct: 186 ETKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWVLGFGIPFIAMVLALLVFLLGTRR 245

Query: 244 YRYSNRGAEENPFVRIGRVFITALRNWHLSPSEMAHEQETPVLLAHRTSQQFR 292
           YRYSN G EENPFVRIGRVFITALRNW L+ SE+AHE+E   LL H +S+QFR
Sbjct: 246 YRYSNTGDEENPFVRIGRVFITALRNWRLTSSEIAHEEEIRGLLPHHSSRQFR 296

BLAST of Moc11g31580 vs. ExPASy TrEMBL
Match: A0A6J1IG54 (protein NRT1/ PTR FAMILY 5.10-like OS=Cucurbita maxima OX=3661 GN=LOC111472583 PE=3 SV=1)

HSP 1 Score: 436.4 bits (1121), Expect = 9.7e-119
Identity = 226/294 (76.87%), Postives = 252/294 (85.71%), Query Frame = 0

Query: 1   MESPLLDDSETVDGAVDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLT 60
           ME+PLL+D  TV+GAVDY+GRP  RS SGGWRSA+ IIGVEV ERFAYYGVSSNLI+FLT
Sbjct: 1   MEAPLLED--TVEGAVDYNGRPVRRSDSGGWRSAALIIGVEVAERFAYYGVSSNLISFLT 60

Query: 61  DQLRQSTATAAENVNALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSA 120
           DQL QSTATAAENVNA SGAASLLPLLGA +ADSFLGRY TI LS+ LYIL LGLLTVSA
Sbjct: 61  DQLGQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSTVLYILGLGLLTVSA 120

Query: 121 TFPSPTISASLHTEQA---SPSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHP 180
           T  SP IS    TE++   SP   Q ILFFSSLY++A AQGGH+PCVQAFGADQFD QHP
Sbjct: 121 TLHSPRISGCQQTEKSLPCSPGLFQVILFFSSLYVVAFAQGGHKPCVQAFGADQFDGQHP 180

Query: 181 QETQAKSSFFNWWYFCISLATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTR 240
           +E++AKSSFFNWWYFCISLATF TVNILNYVQDNLSW LGFGIPCI+M+ AL+VFLLGTR
Sbjct: 181 EESKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWSLGFGIPCISMVLALVVFLLGTR 240

Query: 241 TYRYSNRGAEENPFVRIGRVFITALRNWHLSPSEMAHEQETPVLLAHRTSQQFR 292
           TYR+ NRG EENPFVRIGRVFITA+RNW ++ SE+AHE+E   LL H +SQQFR
Sbjct: 241 TYRFINRGDEENPFVRIGRVFITAVRNWRVNSSEIAHEEEIRGLLPHHSSQQFR 292

BLAST of Moc11g31580 vs. ExPASy TrEMBL
Match: A0A6J1EC56 (protein NRT1/ PTR FAMILY 5.10-like OS=Cucurbita moschata OX=3662 GN=LOC111432731 PE=3 SV=1)

HSP 1 Score: 436.0 bits (1120), Expect = 1.3e-118
Identity = 226/294 (76.87%), Postives = 251/294 (85.37%), Query Frame = 0

Query: 1   MESPLLDDSETVDGAVDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLT 60
           ME+PLL+D  TV+GAVDY GRP  RS SGGWRSA+ IIGVEV ERFAYYGVSSNLINFLT
Sbjct: 1   MEAPLLED--TVEGAVDYHGRPVRRSDSGGWRSAALIIGVEVAERFAYYGVSSNLINFLT 60

Query: 61  DQLRQSTATAAENVNALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSA 120
           DQL QSTATAAENVN  S AASLLPLLGA +ADSFLGRY TI LSS LYIL LGLLTVSA
Sbjct: 61  DQLGQSTATAAENVNVWSAAASLLPLLGAFLADSFLGRYRTIALSSVLYILGLGLLTVSA 120

Query: 121 TFPSPTISASLHTEQA---SPSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHP 180
           T  SP+IS    TE++   SP   Q ILFFSS+Y++A AQGGH+PCVQAFGADQFDAQHP
Sbjct: 121 TLHSPSISGCQQTEKSLPCSPGLFQVILFFSSVYIVAFAQGGHKPCVQAFGADQFDAQHP 180

Query: 181 QETQAKSSFFNWWYFCISLATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTR 240
           +E++AKSSFFNWWYFCISLATF TVNILNYVQDNLSW LGFGIPCI+M+ AL+VFLLGTR
Sbjct: 181 EESKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWSLGFGIPCISMVLALVVFLLGTR 240

Query: 241 TYRYSNRGAEENPFVRIGRVFITALRNWHLSPSEMAHEQETPVLLAHRTSQQFR 292
           TYR+ NRG EENPFVRIGRVFITA+RNW ++ SE+AHE+E   LL H +SQQFR
Sbjct: 241 TYRFINRGDEENPFVRIGRVFITAVRNWRVNSSEIAHEEEICGLLPHHSSQQFR 292

BLAST of Moc11g31580 vs. ExPASy TrEMBL
Match: A0A6J1EHW2 (protein NRT1/ PTR FAMILY 5.10-like OS=Cucurbita moschata OX=3662 GN=LOC111432710 PE=3 SV=1)

HSP 1 Score: 433.0 bits (1112), Expect = 1.1e-117
Identity = 225/294 (76.53%), Postives = 252/294 (85.71%), Query Frame = 0

Query: 1   MESPLLDDSETVDGAVDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLT 60
           ME+PLL+D  TV+ AVDY+GRP  RS SGGWRSA+ IIGVEV ERFAYYGVSSNLI+FLT
Sbjct: 1   MEAPLLED--TVECAVDYNGRPVRRSDSGGWRSAALIIGVEVAERFAYYGVSSNLISFLT 60

Query: 61  DQLRQSTATAAENVNALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSA 120
           DQL QSTATAAENVNA SGAASLLPLLGA +ADSFLGRY TI LS+ LYIL LGLLTVSA
Sbjct: 61  DQLGQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSTVLYILGLGLLTVSA 120

Query: 121 TFPSPTISASLHTEQA---SPSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHP 180
           T  SP+IS    TE++   SP   Q ILFF SLY++A AQGGH+PCVQAFGADQFDAQHP
Sbjct: 121 TLHSPSISGCQQTEKSLPCSPGLFQVILFFFSLYVVAFAQGGHKPCVQAFGADQFDAQHP 180

Query: 181 QETQAKSSFFNWWYFCISLATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTR 240
           +E++AKSSFFNWWYFCISLATF TVNILNYVQDNLSW LGFGIPCI+M+ AL+VFLLGTR
Sbjct: 181 EESKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWSLGFGIPCISMVLALVVFLLGTR 240

Query: 241 TYRYSNRGAEENPFVRIGRVFITALRNWHLSPSEMAHEQETPVLLAHRTSQQFR 292
           TYR+ NRG EENPFVRIGRVFITA+RNW ++ SE+AHE+E   LL H +SQQFR
Sbjct: 241 TYRFINRGDEENPFVRIGRVFITAVRNWRVNSSEIAHEEEIRGLLPHHSSQQFR 292

BLAST of Moc11g31580 vs. TAIR 10
Match: AT1G22540.1 (Major facilitator superfamily protein )

HSP 1 Score: 331.3 bits (848), Expect = 8.5e-91
Identity = 174/275 (63.27%), Postives = 213/275 (77.45%), Query Frame = 0

Query: 16  VDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLTDQLRQSTATAAENVN 75
           VDY  +PA +SSSGGWRSA FIIGVEV ERFAYYG+SSNLI +LT  L QSTA AA NVN
Sbjct: 19  VDYRNKPAVKSSSGGWRSAGFIIGVEVAERFAYYGISSNLITYLTGPLGQSTAAAAANVN 78

Query: 76  ALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSATFPSPTISASLHTEQ 135
           A SG ASLLPLLGA VADSFLGR+ TIL +SALYI+ LG+LT+SA  PS    ++L    
Sbjct: 79  AWSGTASLLPLLGAFVADSFLGRFRTILAASALYIVGLGVLTLSAMIPSDCKVSNL-LSS 138

Query: 136 ASPSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHPQETQAKSSFFNWWYFCIS 195
            SP   Q I FFS+LYL+A+AQGGH+PCVQAFGADQFD + P+E +AKSSFFNWWYF + 
Sbjct: 139 CSP-RFQVITFFSALYLVALAQGGHKPCVQAFGADQFDEKEPEECKAKSSFFNWWYFGMC 198

Query: 196 LATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTRTYRYSNRGAEENPFVRIG 255
             T  T+ +LNY+QDNLSW LGFGIPCIAM+ AL+V LLGT TYR+S R  +++PFVRIG
Sbjct: 199 FGTLTTLWVLNYIQDNLSWALGFGIPCIAMVVALVVLLLGTCTYRFSIRREDQSPFVRIG 258

Query: 256 RVFITALRNWHLSPSEMAHEQETPVLLAHRTSQQF 291
            V++ A++NW +S  ++A  +E   L++  +SQQF
Sbjct: 259 NVYVAAVKNWSVSALDVAAAEERLGLVSCSSSQQF 291

BLAST of Moc11g31580 vs. TAIR 10
Match: AT1G22570.1 (Major facilitator superfamily protein )

HSP 1 Score: 281.6 bits (719), Expect = 7.7e-76
Identity = 147/267 (55.06%), Postives = 190/267 (71.16%), Query Frame = 0

Query: 2   ESPLLDDSETVDGAVDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLTD 61
           E  LL+D   V  +VD+ G PA +SS+GGWRSA FIIGVEV ERFAY+G++ NLI +LT 
Sbjct: 7   EVALLED--YVSDSVDHRGFPAGKSSTGGWRSAWFIIGVEVAERFAYFGIACNLITYLTG 66

Query: 62  QLRQSTATAAENVNALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSAT 121
            L QSTA AA NVN  SG AS+LP+LGA VAD++LGRY TI+++S +YIL LGLLT+SA+
Sbjct: 67  PLGQSTAKAAVNVNTWSGTASILPILGAFVADAYLGRYRTIVVASLIYILGLGLLTLSAS 126

Query: 122 FPSPTISASLHTEQASPSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHPQETQ 181
                +S   +   A PS     LFF SLYL+A+ QGGH+PCVQAFGADQFDA+ P+E  
Sbjct: 127 LIIMGLSKQRNDASAKPSIWVNTLFFCSLYLVAIGQGGHKPCVQAFGADQFDAEDPKEVI 186

Query: 182 AKSSFFNWWYFCISLATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTRTYRY 241
           A+ SFFNWW+  +S    +++ ++ YVQ+N++W  GFGIPC+ M+ AL +FLLG + YRY
Sbjct: 187 ARGSFFNWWFLSLSAGISISIIVVAYVQENVNWAFGFGIPCLFMVMALAIFLLGRKIYRY 246

Query: 242 SNRGAEE----NPFVRIGRVFITALRN 265
                EE    N F RIGRVF+ A +N
Sbjct: 247 PKGHHEEVNSSNTFARIGRVFVIAFKN 271

BLAST of Moc11g31580 vs. TAIR 10
Match: AT1G22550.1 (Major facilitator superfamily protein )

HSP 1 Score: 276.9 bits (707), Expect = 1.9e-74
Identity = 147/268 (54.85%), Postives = 193/268 (72.01%), Query Frame = 0

Query: 2   ESPLLDDSETVDGAVDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLTD 61
           E+ L++DS  V  +VD+ G PA +SS+GGWRSA +IIGVEV ERFAY+G+ SNLI +LT 
Sbjct: 7   EAALIEDS--VSDSVDHRGLPAGKSSTGGWRSAWYIIGVEVGERFAYFGIGSNLITYLTG 66

Query: 62  QLRQSTATAAENVNALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSAT 121
            L QSTATAA NVN  SG AS+LP+LGA +AD++LGRY TI+++S +YIL LGLLT+S+ 
Sbjct: 67  PLGQSTATAAVNVNTWSGTASILPVLGAFIADAYLGRYRTIVVASLIYILGLGLLTLSSI 126

Query: 122 FPSPTISAS-LHTEQAS--PSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHPQ 181
                +S    H   AS  P     ILFF SLYL+A+ QGGH+PCVQAFGADQFD   P+
Sbjct: 127 LILMGLSEQRQHNRNASAKPFFWVNILFFCSLYLVAIGQGGHKPCVQAFGADQFDVGDPK 186

Query: 182 ETQAKSSFFNWWYFCISLATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTRT 241
           E  ++ SFFNWW+  +S    +++ ++ YVQDN++W LGFGIPC+ M+ AL +FL G +T
Sbjct: 187 ERISRGSFFNWWFLSLSAGITLSIIVVVYVQDNVNWALGFGIPCLFMVMALALFLFGRKT 246

Query: 242 YRY--SNRGAEENPFVRIGRVFITALRN 265
           YRY   +R  + N F RIGRVF+ A +N
Sbjct: 247 YRYPRGDREGKNNAFARIGRVFLVAFKN 272

BLAST of Moc11g31580 vs. TAIR 10
Match: AT1G72140.1 (Major facilitator superfamily protein )

HSP 1 Score: 276.6 bits (706), Expect = 2.5e-74
Identity = 145/296 (48.99%), Postives = 204/296 (68.92%), Query Frame = 0

Query: 2   ESPLLDDSETVDGAVDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLTD 61
           E+  +  +E V+ +VD+ G P+ RSSSG W+S+ F +  EV E+FAY+G++SNLI + T+
Sbjct: 9   ETGAIVSNENVEFSVDFRGNPSIRSSSGAWKSSGFTMCAEVAEKFAYFGIASNLITYFTE 68

Query: 62  QLRQSTATAAENVNALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSAT 121
            L +STA AA NVN   G A+ LPL+   +ADSFLGR+ TILL+S+ YI+ LGLLT SAT
Sbjct: 69  ALGESTAVAASNVNLWLGTAAFLPLIWGSIADSFLGRFRTILLTSSFYIMGLGLLTFSAT 128

Query: 122 FPSPTISASLHTEQASPSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHPQETQ 181
            PS  +     T ++  S  + I+FF +LYL+A+ +GG + C++AFGADQFD Q P E++
Sbjct: 129 IPS--LCNDQETRESCVSQVKVIIFFCALYLIALGEGGFKVCLRAFGADQFDEQDPNESK 188

Query: 182 AKSSFFNWWYFCISLATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTRTYRY 241
           AKSS+FNW YF IS+    T  + NYVQ+NLSW LG+ IPC++M+ AL +FLLG +TYR+
Sbjct: 189 AKSSYFNWLYFAISIGILTTRLVTNYVQENLSWALGYAIPCLSMMLALFLFLLGIKTYRF 248

Query: 242 SNRG------AEENPFVRIGRVFITALRNWHLSPSEMAHEQETPVLLAHRTSQQFR 292
           S  G        +NPFVRIGRVF+ A RN   +PS      +T +LL + ++++FR
Sbjct: 249 STGGEGRQGKKHDNPFVRIGRVFVAAARNRRQTPS------DTCLLLPNESTKKFR 296

BLAST of Moc11g31580 vs. TAIR 10
Match: AT1G72120.1 (Major facilitator superfamily protein )

HSP 1 Score: 275.4 bits (703), Expect = 5.5e-74
Identity = 151/295 (51.19%), Postives = 195/295 (66.10%), Query Frame = 0

Query: 10  ETVDGAVDYSGRPAHRSSSGGWRSASFIIGVEVVERFAYYGVSSNLINFLTDQLRQSTAT 69
           E V  AVD+ G  A RS++G WR+A FIIGVEV ERFAYYG+ SNLI++LT  L +STA 
Sbjct: 12  EYVTDAVDHRGLAARRSNTGRWRAALFIIGVEVAERFAYYGIGSNLISYLTGPLGESTAV 71

Query: 70  AAENVNALSGAASLLPLLGALVADSFLGRYPTILLSSALYILALGLLTVSATFPSPTISA 129
           AA NVNA SG A+LLP+LGA VAD+FLGRY TI++SS +Y+L L  LT+SA     T   
Sbjct: 72  AAANVNAWSGIATLLPVLGAFVADAFLGRYRTIIISSLIYVLGLAFLTLSAFLIPNTTEV 131

Query: 130 SLHTEQASPSAAQQILFFSSLYLMAVAQGGHRPCVQAFGADQFDAQHPQETQAKSSFFNW 189
           +     +S S+   +LFF SLYL+A+ Q GH+PCVQAFGADQFD +  QE   +SSFFNW
Sbjct: 132 T-----SSTSSFLNVLFFFSLYLVAIGQSGHKPCVQAFGADQFDEKDSQEKSDRSSFFNW 191

Query: 190 WYFCISLATFVTVNILNYVQDNLSWVLGFGIPCIAMLFALLVFLLGTRTYRYSNRGAEE- 249
           WY  +S      + ++ Y+Q+  SW  GFGIPC+ M+ +L++F+ G R YRYS R  EE 
Sbjct: 192 WYLSLSAGICFAILVVVYIQEEFSWAFGFGIPCVFMVISLVLFVSGRRIYRYSKRRHEEE 251

Query: 250 -NPFVRIGRVFITALRNWHLSPSEMAHEQETPVLLAHRTSQQFRHSLSLQNKESQ 303
            NPF RIGRVF  AL+N  LS S++  + E     +      F  +L + N  SQ
Sbjct: 252 INPFTRIGRVFFVALKNQRLSSSDLC-KVELEANTSPEKQSFFNKALLVPNDSSQ 300

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022155214.17.5e-158100.00protein NRT1/ PTR FAMILY 5.10-like [Momordica charantia][more]
XP_022155213.11.3e-12280.89protein NRT1/ PTR FAMILY 5.10-like [Momordica charantia][more]
XP_023535809.11.8e-11977.21protein NRT1/ PTR FAMILY 5.10-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_0... [more]
XP_023535815.14.0e-11977.21protein NRT1/ PTR FAMILY 5.10-like isoform X7 [Cucurbita pepo subsp. pepo][more]
KAG6592396.15.3e-11977.21Protein NRT1/ PTR FAMILY 5.10, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q0WP011.2e-8963.27Protein NRT1/ PTR FAMILY 5.10 OS=Arabidopsis thaliana OX=3702 GN=NPF5.10 PE=2 SV... [more]
Q9SK991.1e-7455.06Protein NRT1/ PTR FAMILY 5.15 OS=Arabidopsis thaliana OX=3702 GN=NPF5.15 PE=2 SV... [more]
Q9SK962.7e-7354.85Protein NRT1/ PTR FAMILY 5.16 OS=Arabidopsis thaliana OX=3702 GN=NPF5.16 PE=2 SV... [more]
Q9C7U13.5e-7348.99Protein NRT1/ PTR FAMILY 5.12 OS=Arabidopsis thaliana OX=3702 GN=NPF5.12 PE=2 SV... [more]
Q8VZE27.8e-7351.19Protein NRT1/ PTR FAMILY 5.14 OS=Arabidopsis thaliana OX=3702 GN=NPF5.14 PE=2 SV... [more]
Match NameE-valueIdentityDescription
A0A6J1DPL23.7e-158100.00protein NRT1/ PTR FAMILY 5.10-like OS=Momordica charantia OX=3673 GN=LOC11102235... [more]
A0A6J1DMD56.5e-12380.89protein NRT1/ PTR FAMILY 5.10-like OS=Momordica charantia OX=3673 GN=LOC11102235... [more]
A0A6J1IG549.7e-11976.87protein NRT1/ PTR FAMILY 5.10-like OS=Cucurbita maxima OX=3661 GN=LOC111472583 P... [more]
A0A6J1EC561.3e-11876.87protein NRT1/ PTR FAMILY 5.10-like OS=Cucurbita moschata OX=3662 GN=LOC111432731... [more]
A0A6J1EHW21.1e-11776.53protein NRT1/ PTR FAMILY 5.10-like OS=Cucurbita moschata OX=3662 GN=LOC111432710... [more]
Match NameE-valueIdentityDescription
AT1G22540.18.5e-9163.27Major facilitator superfamily protein [more]
AT1G22570.17.7e-7655.06Major facilitator superfamily protein [more]
AT1G22550.11.9e-7454.85Major facilitator superfamily protein [more]
AT1G72140.12.5e-7448.99Major facilitator superfamily protein [more]
AT1G72120.15.5e-7451.19Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 100..293
e-value: 1.6E-38
score: 132.6
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 17..276
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 3..306
e-value: 8.2E-99
score: 333.1
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 21..267
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..30
NoneNo IPR availablePANTHERPTHR11654:SF510FACILITATOR SUPERFAMILY PROTEIN, PUTATIVE ISOFORM 1-RELATEDcoord: 17..276
IPR018456PTR2 family proton/oligopeptide symporter, conserved sitePROSITEPS01022PTR2_1coord: 88..112

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc11g31580.1Moc11g31580.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035442 dipeptide transmembrane transport
biological_process GO:0042939 tripeptide transport
biological_process GO:0006857 oligopeptide transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0071916 dipeptide transmembrane transporter activity
molecular_function GO:0042937 tripeptide transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity