Moc11g30490 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc11g30490
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionRibonuclease H
Locationchr11: 22291069 .. 22293108 (+)
RNA-Seq ExpressionMoc11g30490
SyntenyMoc11g30490
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTGCGAATCTCGGAGGGAACCGGGATAGCATAATTCATGCATCAACAATTGGCGTCGTCTGTGGGAACGATACAGTTCAAGCAAACTTAGCCGAGGCGAAACCCAGATTCGAGAAGAATAGAATGGAGCAAATCGACACGAGCCTTGGTGGAAGCCAGCAACGTTTCGAAAAGAAAAGCCTGAACGAAGCTGCTGACCACTCGGGGGAAGCAAGCTCGAGTCTTAGTCATCGACTAAATCCGACCATCCCCAGTAGGGGAGAACCGACTGAGGAGGTGGATCTATTCCTTTGACATCCGAGGACCGACAAGTCAACATTGGAACCAGGTTGGGGGAGGAAGAAAGCAAAAACCTTATGAACTTCCTCAGAAGCAATTCCGACATGTTCGCATGGTCACATAAGGACATGCCAGAAATCGATCGAGGAGTTATGGTACACCGACTCAACGTCGACGATAGCTGTAAACTAGTGAAACAGAAACGTCTTGCATTCAACCAAGAAAGGATTGAAATTGTGGCTGAACAAGTAGATGAGCTACTGAAAGCAGGCTTCATTCGAGAAGTACATTATCCCCAGTGGATATCCAACGTAGTGCTGGTAAAGAAGCTAAATGGCAAATGGAGAATGTGCATCGACTTTAGAAACCTAAATAAGGCCTATCCAAAGGATTCTTTTCCCTTGCCTTGAATAGATCAACTGGTTGACGCTACCGCTGGACATGAAAGATTAAGTTTCATGGATGCCTACTATGGGTATAATCAAATCAAGATGCATTTACCAGATCAGGAGAAGACCTTTTTTGTCACTAGTTGAGGTTTATATTGCTACACGGTAATGTCGTTCAGGTTGAAGAATGCAAGCGCGACCTACCAACGAATGGTAAATCGAATGTTTGCAGCCTTAATCGGCAAAAATATGGAAGTCTACGTGGATAATATGCTGGTGAATAGTTGAAAAGATAAACAACACGAGAAGGATTTTGCAGAGGCATTTGAAGTTTTAAGAAAGTACAAGATGAAGCTAAATTCTACTAAGTGCACGTTTGGAGTGACCTGAGGCAAGTTCCTAGGATTCCTAATGCACCAAAGGGGAATCAAAGCCAATCCAGATAAGATCAAAGCTGTTTTGGAGATGGATCCACCTAGAAATTTAAAGCAACTACAGGGACTCAAACAAAGGATAGTAGCACTCAACAGATTTGTTAGTCGCTCAATTGACAAGTTCCTGTCGTTCTTCAAAATCCTAAGGAAGAAAAGGCAATTTGAATGGACAGAAGAGTGTGAGGTTGCCTTTAAAGAGCTAAATCAATACCTGGGGGTAGCCCCACTCCTCTCAAAGCCGATCGTTGGTGATGAACTCTACTTGTACTTAGTTGTATCCGACACTGCAGTGAGTTCCACGTTAATAAAAGAAGAAGATGGGCGACAATCTCCAATCTACTACACAAGTAAAGCAATGGTAGATGCAGAATTTAGATACCCACAAGTTGAGAAATTGGCACTAACATTGGTCACTTCGGCTCGGAGATTACGTCCATATTTCTAAGCCTACAGAATAACCGTCCTCACCAATTTTCCTCTACGACAGATCTTGCAAAGGCCAGACACTTCAAGGTGACTGGTAAAGTGGGCGGTGGAGCTCAGCGAGTACGACATTCACTATAAGCCAATAACTGCTATGAAAGGGCAGGCTCTAGCCGATTTCATAGCATAACTAACACCAACAGAACCTCAAGTATGTAGCATCGACCCACAGTTAGGGAAGCAGTTTGACAATGCTGCCTTTCGAGAATTTTGTAGAGAGCTAGGCATTAAGCACATCTGTGAAACGTGACTTTAAAGAAGGTGATCTAGTTTTAAGAAAGATCATACCTGATACCAAAGATTCAAGTAGTGGGGCATTCGGACTGACATGGGAAGGCCCATACAAAGTCACTAAAGTAGTTCGAACGGGCACTTATGCCTTGGAAGACCAACGTGGGAAGGCCTTACCACACCCATGGCATGCTCAGAACCTCAAAAGATACTACCACTAG

mRNA sequence

ATGGGTGCGAATCTCGGAGGGAACCGGGATAGCATAATTCATGCATCAACAATTGGCGTCGTCTGTGGGAACGATACAGTTCAAGCAAACTTAGCCGAGGCGAAACCCAGATTCGAGAAGAATAGAATGGAGCAAATCGACACGAGCCTTGGTGGAAGCCAGCAACGTTTCGAAAAGAAAAGCCTGAACGAAGCTGCTGACCACTCGGGGGAAGCAAGCTCGAGTCTTAGTCATCGACTAAATCCGACCATCCCCAGTAGGGGAGAACCGACTGAGGAGGCATTAAGCACATCTGTGAAACGTGACTTTAAAGAAGGTGATCTAGTTTTAAGAAAGATCATACCTGATACCAAAGATTCAAGTAGTGGGGCATTCGGACTGACATGGGAAGGCCCATACAAAGTCACTAAAGTAGTTCGAACGGGCACTTATGCCTTGGAAGACCAACGTGGGAAGGCCTTACCACACCCATGGCATGCTCAGAACCTCAAAAGATACTACCACTAG

Coding sequence (CDS)

ATGGGTGCGAATCTCGGAGGGAACCGGGATAGCATAATTCATGCATCAACAATTGGCGTCGTCTGTGGGAACGATACAGTTCAAGCAAACTTAGCCGAGGCGAAACCCAGATTCGAGAAGAATAGAATGGAGCAAATCGACACGAGCCTTGGTGGAAGCCAGCAACGTTTCGAAAAGAAAAGCCTGAACGAAGCTGCTGACCACTCGGGGGAAGCAAGCTCGAGTCTTAGTCATCGACTAAATCCGACCATCCCCAGTAGGGGAGAACCGACTGAGGAGGCATTAAGCACATCTGTGAAACGTGACTTTAAAGAAGGTGATCTAGTTTTAAGAAAGATCATACCTGATACCAAAGATTCAAGTAGTGGGGCATTCGGACTGACATGGGAAGGCCCATACAAAGTCACTAAAGTAGTTCGAACGGGCACTTATGCCTTGGAAGACCAACGTGGGAAGGCCTTACCACACCCATGGCATGCTCAGAACCTCAAAAGATACTACCACTAG

Protein sequence

MGANLGGNRDSIIHASTIGVVCGNDTVQANLAEAKPRFEKNRMEQIDTSLGGSQQRFEKKSLNEAADHSGEASSSLSHRLNPTIPSRGEPTEEALSTSVKRDFKEGDLVLRKIIPDTKDSSSGAFGLTWEGPYKVTKVVRTGTYALEDQRGKALPHPWHAQNLKRYYH
Homology
BLAST of Moc11g30490 vs. NCBI nr
Match: XP_022156561.1 (uncharacterized protein LOC111023436 [Momordica charantia])

HSP 1 Score: 132.9 bits (333), Expect = 2.6e-27
Identity = 61/69 (88.41%), Postives = 66/69 (95.65%), Query Frame = 0

Query: 100 KRDFKEGDLVLRKIIPDTKDSSSGAFGLTWEGPYKVTKVVRTGTYALEDQRGKALPHPWH 159
           KRDFKEGDLVLRKI+P+TKDS+SGAF  TWEGPYKVTKVVRTGTYALEDQ+GKAL HPWH
Sbjct: 158 KRDFKEGDLVLRKIMPNTKDSTSGAFRPTWEGPYKVTKVVRTGTYALEDQQGKALLHPWH 217

Query: 160 AQNLKRYYH 169
           AQ+LKRYYH
Sbjct: 218 AQHLKRYYH 226

BLAST of Moc11g30490 vs. NCBI nr
Match: XP_022158579.1 (uncharacterized protein LOC111025033 [Momordica charantia])

HSP 1 Score: 128.6 bits (322), Expect = 4.9e-26
Identity = 58/69 (84.06%), Postives = 63/69 (91.30%), Query Frame = 0

Query: 100 KRDFKEGDLVLRKIIPDTKDSSSGAFGLTWEGPYKVTKVVRTGTYALEDQRGKALPHPWH 159
           KRDFKEGDLVLRK++P+TKDS+SGA G TWEGPYK TKVVR GTYALEDQRGKAL HPWH
Sbjct: 819 KRDFKEGDLVLRKVMPNTKDSTSGALGPTWEGPYKXTKVVRIGTYALEDQRGKALLHPWH 878

Query: 160 AQNLKRYYH 169
            Q+LKRYYH
Sbjct: 879 XQHLKRYYH 887

BLAST of Moc11g30490 vs. NCBI nr
Match: XP_022155342.1 (uncharacterized protein LOC111022476 [Momordica charantia])

HSP 1 Score: 102.1 bits (253), Expect = 4.9e-18
Identity = 49/58 (84.48%), Postives = 52/58 (89.66%), Query Frame = 0

Query: 100 KRDFKEGDLVLRKIIPDTKDSSSGAFGLTWEGPYKVTKVVRTGTYALEDQRGKALPHP 158
           KRDFKEGDLVLRKI+ +TKDS S AFG TWEGPYKVTKVVR GTYALEDQRG+AL HP
Sbjct: 654 KRDFKEGDLVLRKIMLNTKDSISDAFGPTWEGPYKVTKVVRIGTYALEDQRGEALLHP 711

BLAST of Moc11g30490 vs. NCBI nr
Match: XP_021827603.1 (uncharacterized protein LOC110768210 [Prunus avium])

HSP 1 Score: 93.6 bits (231), Expect = 1.8e-15
Identity = 38/68 (55.88%), Postives = 52/68 (76.47%), Query Frame = 0

Query: 100  KRDFKEGDLVLRKIIPDTKDSSSGAFGLTWEGPYKVTKVVRTGTYALEDQRGKALPHPWH 159
            +R+F+ GDLVLRK++P T+D ++G  G  WEGPY VT V R GTY L    GKALPHPW+
Sbjct: 1277 ERNFRPGDLVLRKVLPGTRDPTAGTLGANWEGPYTVTDVARPGTYRLAHLNGKALPHPWN 1336

Query: 160  AQNLKRYY 168
            A++L++Y+
Sbjct: 1337 AEHLRQYF 1344

BLAST of Moc11g30490 vs. NCBI nr
Match: GFY97882.1 (hypothetical protein Acr_12g0004230 [Actinidia rufa])

HSP 1 Score: 93.6 bits (231), Expect = 1.8e-15
Identity = 42/67 (62.69%), Postives = 49/67 (73.13%), Query Frame = 0

Query: 101 RDFKEGDLVLRKIIPDTKDSSSGAFGLTWEGPYKVTKVVRTGTYALEDQRGKALPHPWHA 160
           + F  GDLVLRK+   TK+ + G  G TWEGPYKVTKV R GTY LED  GK LPHPW+A
Sbjct: 68  KSFLLGDLVLRKVTLSTKEPNVGKLGPTWEGPYKVTKVSRPGTYWLEDMNGKTLPHPWNA 127

Query: 161 QNLKRYY 168
            +LK+YY
Sbjct: 128 DHLKKYY 134

BLAST of Moc11g30490 vs. ExPASy TrEMBL
Match: A0A6J1DVB6 (uncharacterized protein LOC111023436 OS=Momordica charantia OX=3673 GN=LOC111023436 PE=4 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 1.3e-27
Identity = 61/69 (88.41%), Postives = 66/69 (95.65%), Query Frame = 0

Query: 100 KRDFKEGDLVLRKIIPDTKDSSSGAFGLTWEGPYKVTKVVRTGTYALEDQRGKALPHPWH 159
           KRDFKEGDLVLRKI+P+TKDS+SGAF  TWEGPYKVTKVVRTGTYALEDQ+GKAL HPWH
Sbjct: 158 KRDFKEGDLVLRKIMPNTKDSTSGAFRPTWEGPYKVTKVVRTGTYALEDQQGKALLHPWH 217

Query: 160 AQNLKRYYH 169
           AQ+LKRYYH
Sbjct: 218 AQHLKRYYH 226

BLAST of Moc11g30490 vs. ExPASy TrEMBL
Match: A0A6J1DZU0 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111025033 PE=4 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 2.4e-26
Identity = 58/69 (84.06%), Postives = 63/69 (91.30%), Query Frame = 0

Query: 100 KRDFKEGDLVLRKIIPDTKDSSSGAFGLTWEGPYKVTKVVRTGTYALEDQRGKALPHPWH 159
           KRDFKEGDLVLRK++P+TKDS+SGA G TWEGPYK TKVVR GTYALEDQRGKAL HPWH
Sbjct: 819 KRDFKEGDLVLRKVMPNTKDSTSGALGPTWEGPYKXTKVVRIGTYALEDQRGKALLHPWH 878

Query: 160 AQNLKRYYH 169
            Q+LKRYYH
Sbjct: 879 XQHLKRYYH 887

BLAST of Moc11g30490 vs. ExPASy TrEMBL
Match: A0A6J1DP30 (uncharacterized protein LOC111022476 OS=Momordica charantia OX=3673 GN=LOC111022476 PE=4 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 2.4e-18
Identity = 49/58 (84.48%), Postives = 52/58 (89.66%), Query Frame = 0

Query: 100 KRDFKEGDLVLRKIIPDTKDSSSGAFGLTWEGPYKVTKVVRTGTYALEDQRGKALPHP 158
           KRDFKEGDLVLRKI+ +TKDS S AFG TWEGPYKVTKVVR GTYALEDQRG+AL HP
Sbjct: 654 KRDFKEGDLVLRKIMLNTKDSISDAFGPTWEGPYKVTKVVRIGTYALEDQRGEALLHP 711

BLAST of Moc11g30490 vs. ExPASy TrEMBL
Match: A0A2N9EPX2 (Ribonuclease H OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS4754 PE=4 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 2.9e-16
Identity = 45/73 (61.64%), Postives = 53/73 (72.60%), Query Frame = 0

Query: 96   STSVK-RDFKEGDLVLRKIIPDTKDSSSGAFGLTWEGPYKVTKVVRTGTYALEDQRGKAL 155
            +T VK R F+ GD VLRK+   TKDSS G  GL WEGPYKV +  R GTY LED+ GK L
Sbjct: 1486 NTKVKLRRFEIGDWVLRKVTQATKDSSQGKLGLNWEGPYKVIQYYRRGTYHLEDRHGKKL 1545

Query: 156  PHPWHAQNLKRYY 168
            PHPW+A++LK YY
Sbjct: 1546 PHPWNAEHLKNYY 1558

BLAST of Moc11g30490 vs. ExPASy TrEMBL
Match: A0A2N9F7K6 (Reverse transcriptase OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS10626 PE=4 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 6.5e-16
Identity = 44/73 (60.27%), Postives = 53/73 (72.60%), Query Frame = 0

Query: 96   STSVK-RDFKEGDLVLRKIIPDTKDSSSGAFGLTWEGPYKVTKVVRTGTYALEDQRGKAL 155
            +T VK R F+ GD VLRK+   TKD S G  G  WEGPYKVT+  R GTY LED+ GK L
Sbjct: 1657 NTKVKLRRFEVGDWVLRKVTQATKDPSQGKLGPNWEGPYKVTQYYRRGTYHLEDRHGKKL 1716

Query: 156  PHPWHAQNLKRYY 168
            PHPW+A++LK+YY
Sbjct: 1717 PHPWNAEHLKKYY 1729

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022156561.12.6e-2788.41uncharacterized protein LOC111023436 [Momordica charantia][more]
XP_022158579.14.9e-2684.06uncharacterized protein LOC111025033 [Momordica charantia][more]
XP_022155342.14.9e-1884.48uncharacterized protein LOC111022476 [Momordica charantia][more]
XP_021827603.11.8e-1555.88uncharacterized protein LOC110768210 [Prunus avium][more]
GFY97882.11.8e-1562.69hypothetical protein Acr_12g0004230 [Actinidia rufa][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DVB61.3e-2788.41uncharacterized protein LOC111023436 OS=Momordica charantia OX=3673 GN=LOC111023... [more]
A0A6J1DZU02.4e-2684.06Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111025033 PE=4 SV=1[more]
A0A6J1DP302.4e-1884.48uncharacterized protein LOC111022476 OS=Momordica charantia OX=3673 GN=LOC111022... [more]
A0A2N9EPX22.9e-1661.64Ribonuclease H OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS4754 PE=4 SV=1[more]
A0A2N9F7K66.5e-1660.27Reverse transcriptase OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS10626 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 67..84
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 60..98

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc11g30490.1Moc11g30490.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015074 DNA integration
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component GO:0030430 host cell cytoplasm
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity