Moc11g29270 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc11g29270
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionMannan endo-1,4-beta-mannosidase
Locationchr11: 21310363 .. 21316780 (+)
RNA-Seq ExpressionMoc11g29270
SyntenyMoc11g29270
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGTGGTTGGTTTGGGGAGTGCTGTTGGTCCTCCTCGGTGGCCATTCGTCTTCATCGTCTCCGGCGCCGGCGCCGGCGGCCTTCGTCGGAATCGGAGACGGCCATTTTCAGTTGAACGGTTCTCCATTTGTGTTCAATGGATTCAACTCTTATTGGATGATGTCCGTCGGAGCTGACCCAAACCAACGCCGGAAAGTCTCCGATGCCTTTCGCGACGCTGCCGCCGCCGGCCTTACCGTCTGCCGGACTTGGGCCTTCGCCGACGGCGGCTATCACGCCCTCCAAATCTCTCCCGGCGTTTACGATGAATCGGTTTTTCAGGTTTTTCCTTTTTTTTTTCCTTTTCTTTTTCCTCTTTTAATCATATTAGAATTTTAAATATGTAATTTCATCATTTCACGTCCAACTTCTGTATTAATATTTTTAATAATTTTTTTAACACTTTTTTTATTAATACATTTGCTCTTTCAACTTTTCCATGTTGTGCTTATTTTATTAAATTCTCCTCCTTAACTTTTAAGATTTATCGTATTTTATTCGTATACTTCAAAAGTTTCTTAATTTTAATAATGGGTTTACTATTTTTTATAGTACAATATATTGGGATATAAGAATTTGAATCTCTGACCTCAAGAAATTATTAGTAGTGTCTGTTGAGCTATGTTCATATTGACGTGTCTAGTATTTCTTTGTGAATTTCGTCGGTTTAACATTTATAATCTTTATGTTGATTACCTTGTATTATAGTTTTAATTATTACATTGTGAGATGATTCCAAATCAGTAGTTCGGATATTTGGTTGCATATATATGTAATTAAATTGACGAAGCTATAAAGACAAGAAAATTGAAATCCAAGACCTATCATAATTTTTTGGGAAATTATACTAAATGACATAATTAATATATATATAATTACTTTTATTTTTATTTTAAAATATGTCACAGAAATGATTTTTGGTTGGTCTATGTGATTATATGTTTTGTCATTTATATCATTTTTATAAAATTTTAAACTTCTATATATAAAAATACATATTCCATATTATTTTTGTTTTTTTAAGATACATGAACTAAATAGATTCAATTTGGGTTGATTATATGTTTTGTCATTTATATGATTTTTGTAAAATTTTAAACTTCAATATATAAAAATACATATTCCGTGTTATTTTTGATTTTTTTAGGTACATGAACTAAGTAGATTCAATGTCAAAGTTAAAGATTTATTAAATTTTTTTGTTTTTAAAATAATAGAGATTGAATATTTATTAAATTTTTTTGTTTTTAAAATAATAGAGATTGAATAGATATAATCATAACTGAGATGTCTTAATATTTTAATATTTTAACATTTTTAATTTTATCTATTTTTCGTATAAAAAAATTTCAGGCGTTTATTTTTCGTTGAAACCCTAGGATTTCAAAACGTTTTCGGAATAAATTAGAACAGAAAGAAAGCAATTAATAAGTAAACATGATTTTTAAAAATTTGAAATACTAAAAATAAAAACATTATCAAAGAGCTCTTATTTTTATGAAATAAAATAAAATAAAAGTTTAAATTCTATTTTAGTTTATAGTTTTATATTTTGTCCCTAAATTTTTAAATATTTTGTGTTAACTTTCATAATTTTTTCTTTTAATCTCTTGACATTCAATTCTTTTATTTTTTATCTTTGAACTTTGCTTTTAAAAAGTACTACTTTAGTCCCTACCACTAATTTTCATTAATCGTTTAATAATAAACTAGACACAGTCTATTACACATATGTTTTAAACATTCGTCCACGTAAATATCTATCGATCAAGTATATGCACAAAAAATGTGACAATATCATATTTCAGTTAAATATTAATTTATTAAAATATTAACAATTAATAATAAAATAGTTTTTTAATTAAAAGTTTAATAAGACAAAACATTACTAATCTATATAATCAACAATTACCTTCACCTGCTATTGCGAGTATAACTTAATGGTAATTGACATATATCTCAAATCAAGAGGTGTGAGTTCAATCTCCCACACCCCACATATTGTACTAAAAAAATATGTCACCTTCACCCCGATTTGAACTCAAAAAAATTAAATATTTAAAAGTGGAAGAATAGAAAATGTTAAATATATGAAATGATACTTAATTTTTTCAATAATTCTCCATTTGATTCTCAATAATTTAAAATTCTCAAATTTACCTTTAATGTATGAAAATTCTCAATTAGGCCATTCCATTATTTTTTTTTAACTTGTTAACAAAAATTTAATGATTTATTGTTTTATTAACATATTTGGACACATAAATATATTACTTGAGTGTCATTTGACACATGAAGTCCAACTTTCTCCTCAACAAAACTATATTCTTTGAACTCACTTGTCAGGTTAACAAAATAATAAATCTAATGTCGTATCAAGTTTTCGTAAATAGATTTTAACATATTTTTCAATGAAATAACTCCATTGAGAATTCATAATGCAAGTAAAATTGAGGAGAAAAAATTTTCTAAATTTTGATAAGAGTTATTGAAGGCTTAATTCCATTGAAATTAGTAAGTTTTTTGATATCTTTACCTTACCTTTTGGGTGAATTAGCAAAAATGAAAGTTCTCTTTTTTTTTTCTCTCTTTTTTAAGAACTGAAAATAAAGATTAAAAATCAAAACCGCCGTCGCTTTGATCAGGCTCTTGATTTTGTGATATGGGAGGCAAGAAAATACGGAATTTGGATAATATTGAGTTTGGTGAATAATTACGGCGACTACGGCGGAAGGCCGCAGTACGTCCGGTGGGCCCGGGCTGCCGGCGCTCAAATTCACAACGACGATGACTTCTATACAAATCAACTCATCAAGGGTTTCTACACGGGCCACGTCGAGGTAATCCCGTCTTTTAATTTCTATTTTGGTTTCTAAACTCTTAGAGTAAATTACATATTTTGAATATTGAGTTTGTTATTTAGTCTCTAAATTTTATAATGTTTCAATTCCGTTTGTAGAAAATTTTGAATCGGACTTATCTTTTGTCTCTACCGTCAATAATGGTTTGATAATTGGGTGGGTGTGACACTCGATCGAGTAATTAGTTTCATGGATTAGAAATTAATGGACAGAGATAGATGTTTGGTTATTGATTTAGCAAATAACTGAGTTGAGTTGTATTTCACTCCTAACCACAACTTATCTGCTAATTTTCCAACATCATTAGTCAATTTGGTTATCCACTTAGTATCACCCAATCTTCATCCTGGTCATAGATAAGTCACCCAAATTCAAGTTTATAAGCAAAGAGTGCCAATTGGCCTCATGAAGATTCAATTTCACTGTGGCTAAGTCATTGTTTATGAGTCGTCAATTATTCTTCAACAAACATGTAGTCAGAGGTCCTGGTCTCCTCCTACTATTTAGAAGTGCTTACGGTTTCATGTTCATCGGCATGTAGGTCCTAAGAAAAGGTTTAGAATAATCAATTCGGTGATTCTATCAACCAATGTTGTTCATATCATGTATTAAAGATAAGTATGATTCTCCGAGTACCACTTAAAAAAATTATACCTTCCTTTTACAATCAGCAAAATAGGTAAGTTTGATTTATTAATTATTTCTAATCTAAGATCAACCTATCACTCCCATAGATTGTTAAGATTAATTTGAATATAGCTCAGCAAGTTAAGATATTTATATCTGACTAAAAAGTCGTGAGTTCGAATTGATGTTGTACTTAAAAAAACATAGATCGTTAAATTGTTGCTTTTAAAATTTTGCAGAAAGTGTTGACAAGGAAGAACACAATGAGCGGCTTAAGGTATATGGATGATCCCACGATTATGGGATGGGAACTCATGAACGAACCACGTTGTGAAGTTGATTCCTCTGGCGCCACTGTTAATGTGAGCCCTACCATTTTATTATATATTTATTGCATCATCATTTTATTTTTAATTACCGTCATATTATACTTTCAGTCCATCATTTTTTCATTGAGCGAAAGGTACGAAATAAATCAGATTGATTTTTCGATCAAAAGTACTATGTGAAATCTTCGTTTTTGTCCTCTTTCTCTATCCCTATCTCGCAGGTACAGTGTTTGAATCAATAGAGAACCTTTTGTTCTGTATCTGTATGAATCGCATTATTGCATTCCAATTCCTTCCCGATACCTCCCAAGGAAAATTCCAAATTGGATCCAAAATTGACGGGTTAGTGTGGTTTGGACTTTGATTTATTTTGGTTCCTTTCCTAAGTTTCCAAAACTAACACTATTGCTCCAGAAAACTCGAATTTAGTTTTCATTTAGTCCCTCGTATTTTAAAATGATACTTTTGGTCTTTGAGGTTTGGATTTAATTTCTATTTAGTCCCTCCAATTTCAGAAATGACATTTTTTGTTTTTCACATTTGGATTTTATTTTTAGTTGGTCTTTGAAGTTTCAAAAGCAACACTTTTAGTCCTTAGAGTTCGGATTTTAATTTCTATTTAGTCTCCAACCCTAAGTTAAGTTAACAGATAGATGACGTGACAATTTACTTCTTCCTGGAGGTCTTCGATTGGTTGAGAACGTCGACATACCCATTAACAGTGACATGGGTGGTCATCAATAGTCCACTTCAACATGATATCTACTGCATCGTCATTTTTCTAAGTAAACTAATGACAAACTCAATTCAAGGACTATATAAAAATCAAATCCAAACCTCAAAAGACCAAATAAAAGCAAAATTTAAACCTTAGAAACTAAAAGTTTAAGTGTAAAAACTTCCAAGATCAAATACAATCTAAATTCAAACTTTGGTAACATATATGCACCATTAATTACTATTTGGTTAGTATATTGACTAGTACAACCAATGTCTGATTGAAATCAATCCACTATTGACTATATCCAATACGAGTCAAATTTTAACTTATATCAATTACTTGAATGATCAATGTTTTAGCTTCTCTATATCAATATTCACGGAGTTTCATCTCAGAACCAATTGACAATGAGAGAAATAACCCACTTATATTATAAATTCTGGTTAGATCCCTTAACGTAGTATCCTTAACATGACCCCTCGAAATGGTGCCTCCACCAATCTTGGTCAGATCTCATTTTCAGATTGGATACTCGTTTGAACTTTTGAGCTCTGAATAAACCGCTACTCAACCAAAAAACTTAAGTTGGTGGATTTGATAAATTCAATTCATATTTTAATATCTAACCTTCTCTAATTTGAGCGTGTTTGTGATGTGATAGAGATGGGTGGAAGAAATGGGAACTTATGTGAAGTCAATTGACAAAAAACATTTGGTGGGCATTGGAATGGAAGGATTCTATGGAGATTCAAACCCAAGTAGAATCAAATTCAACCCAAATGGTTACAAAGTTGGCACTGATTTTGTGACCAACAATCTCATCAAAGTTATAGACTTTGCAACAATACATGCATATCCTGATTCTTGGTCAGTCCTTTCATTCTCTCTCTATATTTCTCCCTCTAAATTCTAATTTCTAATCACCTATTTGATCATTATTTTGTTTTTAAAAGTTGTGAAATGCTAATTATATTTCTTTGTTTTGAAATCTACACATTTTCCAGGTTTATTTAATAACAACTTGAGTTCTTATCCCAAAAAAAAAAAAAAAATTGTGTTTCAGGCTTTTGATTTGTGAAAATAAAATCATATTTACCAATATAATTATATATTCTTAAATTATATTCTAAAAACAAAAACCTCATTTGATATTTGATATTTGATAACAATTATATTCTTAAATTTTCTATTTTATTATAAAGAATAAGCTCAAGTCATTTACTTGAACACTTTCGAATGAAATCGTAGTTTTTAGCCCAATTTCAAAAATTGAAACACAAACTTGTGATTTTATGTATTATTTCTTTTCGAGTTCTCAACATCTAGCTAAGAGAAAATTTAGTATAATCATTGAAAATAGAAAGCCGAAACATTATCAAGCCCAGAATAGAAAAATTACTATCAAACGGGACCTTCAAAATCGACCCTTGAAAAAGAAAGCATTGTAATTTGTTCTCCATTTTCACAGGCTACCAGGACAGAGTGAGGCAACCAAAATGGCATTCTTGGAGGAGTGGATGAAAATTCACTGGTCAGACTCAAAGACAATCTTAAAGAAGCCATTGATTTTTGCAGAATTTGGAAAGTCAACCAAAAACCAAAACCAAAACCAGGCATTCAGTGTAAGAGACAGAGATTCATTCCTCACTAAGGTTTATTCAAACATCTACAACCTTGCAAGAAATGGGACAACCATGGCTGGAGGTTTGGTCTGGCAAGTTATGGCTGAAGGAATGGAGTCATACTACGATGGATACGAGATCGTTCTTTCCCAGAATCCTTTGACAACCGCCATTATAATGCAACAATCAAAAAACATGGCTGCCCTTCAACATTAA

mRNA sequence

ATGAGGTGGTTGGTTTGGGGAGTGCTGTTGGTCCTCCTCGGTGGCCATTCGTCTTCATCGTCTCCGGCGCCGGCGCCGGCGGCCTTCGTCGGAATCGGAGACGGCCATTTTCAGTTGAACGGTTCTCCATTTGTGTTCAATGGATTCAACTCTTATTGGATGATGTCCGTCGGAGCTGACCCAAACCAACGCCGGAAAGTCTCCGATGCCTTTCGCGACGCTGCCGCCGCCGGCCTTACCGTCTGCCGGACTTGGGCCTTCGCCGACGGCGGCTATCACGCCCTCCAAATCTCTCCCGGCGTTTACGATGAATCGGTTTTTCAGGCTCTTGATTTTGTGATATGGGAGGCAAGAAAATACGGAATTTGGATAATATTGAGTTTGGTGAATAATTACGGCGACTACGGCGGAAGGCCGCAGTACGTCCGGTGGGCCCGGGCTGCCGGCGCTCAAATTCACAACGACGATGACTTCTATACAAATCAACTCATCAAGGGTTTCTACACGGGCCACGTCGAGAAAGTGTTGACAAGGAAGAACACAATGAGCGGCTTAAGGTATATGGATGATCCCACGATTATGGGATGGGAACTCATGAACGAACCACGTTGTGAAGTTGATTCCTCTGGCGCCACTGTTAATAGATGGGTGGAAGAAATGGGAACTTATGTGAAGTCAATTGACAAAAAACATTTGGTGGGCATTGGAATGGAAGGATTCTATGGAGATTCAAACCCAAGTAGAATCAAATTCAACCCAAATGGTTACAAAGTTGGCACTGATTTTGTGACCAACAATCTCATCAAAGTTATAGACTTTGCAACAATACATGCATATCCTGATTCTTGGCTACCAGGACAGAGTGAGGCAACCAAAATGGCATTCTTGGAGGAGTGGATGAAAATTCACTGGTCAGACTCAAAGACAATCTTAAAGAAGCCATTGATTTTTGCAGAATTTGGAAAGTCAACCAAAAACCAAAACCAAAACCAGGCATTCAGTGTAAGAGACAGAGATTCATTCCTCACTAAGGTTTATTCAAACATCTACAACCTTGCAAGAAATGGGACAACCATGGCTGGAGGTTTGGTCTGGCAAGTTATGGCTGAAGGAATGGAGTCATACTACGATGGATACGAGATCGTTCTTTCCCAGAATCCTTTGACAACCGCCATTATAATGCAACAATCAAAAAACATGGCTGCCCTTCAACATTAA

Coding sequence (CDS)

ATGAGGTGGTTGGTTTGGGGAGTGCTGTTGGTCCTCCTCGGTGGCCATTCGTCTTCATCGTCTCCGGCGCCGGCGCCGGCGGCCTTCGTCGGAATCGGAGACGGCCATTTTCAGTTGAACGGTTCTCCATTTGTGTTCAATGGATTCAACTCTTATTGGATGATGTCCGTCGGAGCTGACCCAAACCAACGCCGGAAAGTCTCCGATGCCTTTCGCGACGCTGCCGCCGCCGGCCTTACCGTCTGCCGGACTTGGGCCTTCGCCGACGGCGGCTATCACGCCCTCCAAATCTCTCCCGGCGTTTACGATGAATCGGTTTTTCAGGCTCTTGATTTTGTGATATGGGAGGCAAGAAAATACGGAATTTGGATAATATTGAGTTTGGTGAATAATTACGGCGACTACGGCGGAAGGCCGCAGTACGTCCGGTGGGCCCGGGCTGCCGGCGCTCAAATTCACAACGACGATGACTTCTATACAAATCAACTCATCAAGGGTTTCTACACGGGCCACGTCGAGAAAGTGTTGACAAGGAAGAACACAATGAGCGGCTTAAGGTATATGGATGATCCCACGATTATGGGATGGGAACTCATGAACGAACCACGTTGTGAAGTTGATTCCTCTGGCGCCACTGTTAATAGATGGGTGGAAGAAATGGGAACTTATGTGAAGTCAATTGACAAAAAACATTTGGTGGGCATTGGAATGGAAGGATTCTATGGAGATTCAAACCCAAGTAGAATCAAATTCAACCCAAATGGTTACAAAGTTGGCACTGATTTTGTGACCAACAATCTCATCAAAGTTATAGACTTTGCAACAATACATGCATATCCTGATTCTTGGCTACCAGGACAGAGTGAGGCAACCAAAATGGCATTCTTGGAGGAGTGGATGAAAATTCACTGGTCAGACTCAAAGACAATCTTAAAGAAGCCATTGATTTTTGCAGAATTTGGAAAGTCAACCAAAAACCAAAACCAAAACCAGGCATTCAGTGTAAGAGACAGAGATTCATTCCTCACTAAGGTTTATTCAAACATCTACAACCTTGCAAGAAATGGGACAACCATGGCTGGAGGTTTGGTCTGGCAAGTTATGGCTGAAGGAATGGAGTCATACTACGATGGATACGAGATCGTTCTTTCCCAGAATCCTTTGACAACCGCCATTATAATGCAACAATCAAAAAACATGGCTGCCCTTCAACATTAA

Protein sequence

MRWLVWGVLLVLLGGHSSSSSPAPAPAAFVGIGDGHFQLNGSPFVFNGFNSYWMMSVGADPNQRRKVSDAFRDAAAAGLTVCRTWAFADGGYHALQISPGVYDESVFQALDFVIWEARKYGIWIILSLVNNYGDYGGRPQYVRWARAAGAQIHNDDDFYTNQLIKGFYTGHVEKVLTRKNTMSGLRYMDDPTIMGWELMNEPRCEVDSSGATVNRWVEEMGTYVKSIDKKHLVGIGMEGFYGDSNPSRIKFNPNGYKVGTDFVTNNLIKVIDFATIHAYPDSWLPGQSEATKMAFLEEWMKIHWSDSKTILKKPLIFAEFGKSTKNQNQNQAFSVRDRDSFLTKVYSNIYNLARNGTTMAGGLVWQVMAEGMESYYDGYEIVLSQNPLTTAIIMQQSKNMAALQH
Homology
BLAST of Moc11g29270 vs. NCBI nr
Match: XP_022141751.1 (mannan endo-1,4-beta-mannosidase 5-like [Momordica charantia])

HSP 1 Score: 845.5 bits (2183), Expect = 1.9e-241
Identity = 405/405 (100.00%), Postives = 405/405 (100.00%), Query Frame = 0

Query: 1   MRWLVWGVLLVLLGGHSSSSSPAPAPAAFVGIGDGHFQLNGSPFVFNGFNSYWMMSVGAD 60
           MRWLVWGVLLVLLGGHSSSSSPAPAPAAFVGIGDGHFQLNGSPFVFNGFNSYWMMSVGAD
Sbjct: 1   MRWLVWGVLLVLLGGHSSSSSPAPAPAAFVGIGDGHFQLNGSPFVFNGFNSYWMMSVGAD 60

Query: 61  PNQRRKVSDAFRDAAAAGLTVCRTWAFADGGYHALQISPGVYDESVFQALDFVIWEARKY 120
           PNQRRKVSDAFRDAAAAGLTVCRTWAFADGGYHALQISPGVYDESVFQALDFVIWEARKY
Sbjct: 61  PNQRRKVSDAFRDAAAAGLTVCRTWAFADGGYHALQISPGVYDESVFQALDFVIWEARKY 120

Query: 121 GIWIILSLVNNYGDYGGRPQYVRWARAAGAQIHNDDDFYTNQLIKGFYTGHVEKVLTRKN 180
           GIWIILSLVNNYGDYGGRPQYVRWARAAGAQIHNDDDFYTNQLIKGFYTGHVEKVLTRKN
Sbjct: 121 GIWIILSLVNNYGDYGGRPQYVRWARAAGAQIHNDDDFYTNQLIKGFYTGHVEKVLTRKN 180

Query: 181 TMSGLRYMDDPTIMGWELMNEPRCEVDSSGATVNRWVEEMGTYVKSIDKKHLVGIGMEGF 240
           TMSGLRYMDDPTIMGWELMNEPRCEVDSSGATVNRWVEEMGTYVKSIDKKHLVGIGMEGF
Sbjct: 181 TMSGLRYMDDPTIMGWELMNEPRCEVDSSGATVNRWVEEMGTYVKSIDKKHLVGIGMEGF 240

Query: 241 YGDSNPSRIKFNPNGYKVGTDFVTNNLIKVIDFATIHAYPDSWLPGQSEATKMAFLEEWM 300
           YGDSNPSRIKFNPNGYKVGTDFVTNNLIKVIDFATIHAYPDSWLPGQSEATKMAFLEEWM
Sbjct: 241 YGDSNPSRIKFNPNGYKVGTDFVTNNLIKVIDFATIHAYPDSWLPGQSEATKMAFLEEWM 300

Query: 301 KIHWSDSKTILKKPLIFAEFGKSTKNQNQNQAFSVRDRDSFLTKVYSNIYNLARNGTTMA 360
           KIHWSDSKTILKKPLIFAEFGKSTKNQNQNQAFSVRDRDSFLTKVYSNIYNLARNGTTMA
Sbjct: 301 KIHWSDSKTILKKPLIFAEFGKSTKNQNQNQAFSVRDRDSFLTKVYSNIYNLARNGTTMA 360

Query: 361 GGLVWQVMAEGMESYYDGYEIVLSQNPLTTAIIMQQSKNMAALQH 406
           GGLVWQVMAEGMESYYDGYEIVLSQNPLTTAIIMQQSKNMAALQH
Sbjct: 361 GGLVWQVMAEGMESYYDGYEIVLSQNPLTTAIIMQQSKNMAALQH 405

BLAST of Moc11g29270 vs. NCBI nr
Match: XP_038890634.1 (mannan endo-1,4-beta-mannosidase 1-like [Benincasa hispida])

HSP 1 Score: 672.5 bits (1734), Expect = 2.2e-189
Identity = 323/403 (80.15%), Postives = 354/403 (87.84%), Query Frame = 0

Query: 1   MRWLVWGVLLVLLGGHSSSSSPAPAPAAFVGIGDGHFQLNGSPFVFNGFNSYWMMSVGAD 60
           MRWL+W V  +L G    SSSP+PAP AFVG+ DGHFQLNGSPFVFNGFNSYWMMSV AD
Sbjct: 7   MRWLIWEVFFILSG---LSSSPSPAPEAFVGVQDGHFQLNGSPFVFNGFNSYWMMSVAAD 66

Query: 61  PNQRRKVSDAFRDAAAAGLTVCRTWAFADGGYHALQISPGVYDESVFQALDFVIWEARKY 120
           PNQR KVS+ F DAA+A LTVCRTWAFADGG  ALQISPGVYDE VFQ LDFVI EA+KY
Sbjct: 67  PNQRHKVSNVFHDAASAALTVCRTWAFADGGSPALQISPGVYDEIVFQGLDFVICEAKKY 126

Query: 121 GIWIILSLVNNYGDYGGRPQYVRWARAAGAQIHNDDDFYTNQLIKGFYTGHVEKVLTRKN 180
           GI +ILSLVNNY DYGGR QYVRWA+AAG  I+NDDDFYTNQLIK +Y  HV++VLTRKN
Sbjct: 127 GIRMILSLVNNYKDYGGREQYVRWAKAAGVDIYNDDDFYTNQLIKSYYKNHVQRVLTRKN 186

Query: 181 TMSGLRYMDDPTIMGWELMNEPRCEVDSSGATVNRWVEEMGTYVKSIDKKHLVGIGMEGF 240
           T+SGLRYMDDPTIMGWELMNEPRC++DSSG T+NRWVEEMG+YVKSIDK+HLVGIGMEGF
Sbjct: 187 TISGLRYMDDPTIMGWELMNEPRCQLDSSGNTINRWVEEMGSYVKSIDKQHLVGIGMEGF 246

Query: 241 YGDSNPSRIKFNPNGYKVGTDFVTNNLIKVIDFATIHAYPDSWLPGQSEATKMAFLEEWM 300
            GDS+P++IK NPN Y++GTDF+TNNL K IDFATIHAYPD+WLPGQSEATKMAFLEEW+
Sbjct: 247 CGDSSPTKIKSNPNSYRLGTDFLTNNLNKDIDFATIHAYPDAWLPGQSEATKMAFLEEWI 306

Query: 301 KIHWSDSKTILKKPLIFAEFGKSTKNQNQNQAFSVRDRDSFLTKVYSNIYNLARNGTTMA 360
            IHW+DSKTILKKPLIF EFGKST+ QNQ    +VRDRDSFL+KVYS IYNLARNG TMA
Sbjct: 307 TIHWTDSKTILKKPLIFEEFGKSTRGQNQTS--NVRDRDSFLSKVYSIIYNLARNGATMA 366

Query: 361 GGLVWQVMAEGMESYYDGYEIVLSQNPLTTAIIMQQSKNMAAL 404
           GGLVWQVMAEGMESYYDGYEIVL QNP T AII QQS  MAAL
Sbjct: 367 GGLVWQVMAEGMESYYDGYEIVLPQNPSTNAIITQQSNKMAAL 404

BLAST of Moc11g29270 vs. NCBI nr
Match: XP_008459570.1 (PREDICTED: mannan endo-1,4-beta-mannosidase 1-like [Cucumis melo] >TYK00481.1 mannan endo-1,4-beta-mannosidase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 665.2 bits (1715), Expect = 3.5e-187
Identity = 315/404 (77.97%), Postives = 355/404 (87.87%), Query Frame = 0

Query: 1   MRWLVWGVLLVLLGGHSSSSSPAPAPAAFVGIGDGHFQLNGSPFVFNGFNSYWMMSVGAD 60
           +RWL+WG+LL++    + SSS +P  A FVG+ DGHFQLNGSPF+FNGFNSYWMMSV AD
Sbjct: 8   IRWLIWGLLLLI---STLSSSSSPVTAGFVGVEDGHFQLNGSPFLFNGFNSYWMMSVAAD 67

Query: 61  PNQRRKVSDAFRDAAAAGLTVCRTWAFADGGYHALQISPGVYDESVFQALDFVIWEARKY 120
           PNQR KVS  FRDAA AGLTVCRTWAF DGG+HALQISPGVYDESVFQ LDFVI+EARKY
Sbjct: 68  PNQRHKVSQVFRDAATAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIFEARKY 127

Query: 121 GIWIILSLVNNYGDYGGRPQYVRWARAAGAQIHNDDDFYTNQLIKGFYTGHVEKVLTRKN 180
           GI +ILSLVNN+ DYGGR  YVRWA AAG Q+ N+DDFYTNQLI+ +Y  HV+KVL RKN
Sbjct: 128 GIRMILSLVNNFKDYGGRAAYVRWAEAAGVQVQNEDDFYTNQLIRTYYKNHVQKVLRRKN 187

Query: 181 TMSGLRYMDDPTIMGWELMNEPRCEVDSSGATVNRWVEEMGTYVKSIDKKHLVGIGMEGF 240
           TMS LRYMDDPTIMGWELMNEPRC+VDSSG TVNRWVEEMG+YVKSID++HLVGIGMEGF
Sbjct: 188 TMSCLRYMDDPTIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDRQHLVGIGMEGF 247

Query: 241 YGDSNPSRIKFNPNGYKVGTDFVTNNLIKVIDFATIHAYPDSWLPGQSEATKMAFLEEWM 300
           YGDS P++IK NP+ YKVGTDF+TNNL K ID+ATIHAYPD+WLPG+SE TKMAFLEEW+
Sbjct: 248 YGDSIPTKIKANPSSYKVGTDFITNNLNKAIDYATIHAYPDAWLPGKSETTKMAFLEEWV 307

Query: 301 KIHWSDSKTILKKPLIFAEFGKSTKNQNQNQAFSVRDRDSFLTKVYSNIYNLARNGTTMA 360
            +HW+DSKTILKKPLIF EFG+S +  +QNQ +SVRDRD+FL+KVYS IYNLA+NG TMA
Sbjct: 308 ALHWTDSKTILKKPLIFEEFGRSIR--DQNQTYSVRDRDAFLSKVYSIIYNLAKNGATMA 367

Query: 361 GGLVWQVMAEGMESYYDGYEIVLSQNPLTTAIIMQQSKNMAALQ 405
           GGLVWQVMAEGMESYYDGYEIVLSQNP T AII QQS  MAAL+
Sbjct: 368 GGLVWQVMAEGMESYYDGYEIVLSQNPSTNAIITQQSNKMAALK 406

BLAST of Moc11g29270 vs. NCBI nr
Match: XP_004141536.2 (mannan endo-1,4-beta-mannosidase 1 [Cucumis sativus] >KGN52650.1 hypothetical protein Csa_008142 [Cucumis sativus])

HSP 1 Score: 663.7 bits (1711), Expect = 1.0e-186
Identity = 314/403 (77.92%), Postives = 354/403 (87.84%), Query Frame = 0

Query: 1   MRWLVWGVLLVLLGGHSSSSSPAPAPAAFVGIGDGHFQLNGSPFVFNGFNSYWMMSVGAD 60
           MRWL+W +  ++     S+SS +PAPAAFVG+ DGHFQLNGSPF+FNGFNSYWMMSV  D
Sbjct: 9   MRWLIWALFFLI--STLSASSSSPAPAAFVGVQDGHFQLNGSPFLFNGFNSYWMMSVATD 68

Query: 61  PNQRRKVSDAFRDAAAAGLTVCRTWAFADGGYHALQISPGVYDESVFQALDFVIWEARKY 120
           PNQR KV+  FRDAAAAGLTVCRTWAF DGG+HALQISPGVYDESVFQ LDFVI+EARKY
Sbjct: 69  PNQRNKVTQVFRDAAAAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIFEARKY 128

Query: 121 GIWIILSLVNNYGDYGGRPQYVRWARAAGAQIHNDDDFYTNQLIKGFYTGHVEKVLTRKN 180
           GI +ILSLVNN+ DYGGR  YVRWA AAG Q+H++DDFYTNQLIK +Y  HV+KVL+RKN
Sbjct: 129 GIRMILSLVNNFKDYGGRAAYVRWAEAAGVQVHDEDDFYTNQLIKTYYKNHVQKVLSRKN 188

Query: 181 TMSGLRYMDDPTIMGWELMNEPRCEVDSSGATVNRWVEEMGTYVKSIDKKHLVGIGMEGF 240
           TM+GL YM+D TIMGWELMNEPRC+VDSSG TVNRWVEEMG+YVKSIDK+HLVGIGMEGF
Sbjct: 189 TMNGLIYMEDATIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDKQHLVGIGMEGF 248

Query: 241 YGDSNPSRIKFNPNGYKVGTDFVTNNLIKVIDFATIHAYPDSWLPGQSEATKMAFLEEWM 300
           YGDS+P++IK NP  +K GTDF+TNNL K IDFATIHAYPD+WLPG+SE TKMAFLEEW+
Sbjct: 249 YGDSSPNKIKANPGSFKFGTDFITNNLNKAIDFATIHAYPDAWLPGKSEGTKMAFLEEWI 308

Query: 301 KIHWSDSKTILKKPLIFAEFGKSTKNQNQNQAFSVRDRDSFLTKVYSNIYNLARNGTTMA 360
            +HW+DSKTILKKPLIF EFGKS ++QNQ  + SVRDRD+FL+KV+S IYNLARNG TMA
Sbjct: 309 ALHWTDSKTILKKPLIFEEFGKSIRDQNQTSS-SVRDRDAFLSKVFSIIYNLARNGATMA 368

Query: 361 GGLVWQVMAEGMESYYDGYEIVLSQNPLTTAIIMQQSKNMAAL 404
           GGLVWQVMAEGMESYYDGYEIVLSQ P TTAII  QS NMAAL
Sbjct: 369 GGLVWQVMAEGMESYYDGYEIVLSQTPSTTAIITHQSNNMAAL 408

BLAST of Moc11g29270 vs. NCBI nr
Match: XP_004141631.3 (mannan endo-1,4-beta-mannosidase 1 [Cucumis sativus] >XP_031740837.1 mannan endo-1,4-beta-mannosidase 1 [Cucumis sativus] >KAE8648995.1 hypothetical protein Csa_008431 [Cucumis sativus])

HSP 1 Score: 644.8 bits (1662), Expect = 4.9e-181
Identity = 306/403 (75.93%), Postives = 344/403 (85.36%), Query Frame = 0

Query: 1   MRWLVWGVLLVLLGGHSSSSSPAPAPAAFVGIGDGHFQLNGSPFVFNGFNSYWMMSVGAD 60
           MRW +WG+  ++    + SSS +PAPAAFVG+ D HFQLNGSPF+FNGFNSYWMMSV AD
Sbjct: 8   MRWSIWGIFFLI---STLSSSSSPAPAAFVGVQDDHFQLNGSPFLFNGFNSYWMMSVAAD 67

Query: 61  PNQRRKVSDAFRDAAAAGLTVCRTWAFADGGYHALQISPGVYDESVFQALDFVIWEARKY 120
           PNQR KV+  FRDAA AGLTVCRTWAF DG  HALQISPGVYDESVFQALDFVI+EARKY
Sbjct: 68  PNQRHKVTQVFRDAATAGLTVCRTWAFNDGALHALQISPGVYDESVFQALDFVIFEARKY 127

Query: 121 GIWIILSLVNNYGDYGGRPQYVRWARAAGAQIHNDDDFYTNQLIKGFYTGHVEKVLTRKN 180
           GI ++LSLVNN+ +YGGR  YVRWA AAG Q+H+DDDFYTNQLI+ +Y  +V+KVL RKN
Sbjct: 128 GIRMLLSLVNNFEEYGGRAAYVRWAEAAGVQVHSDDDFYTNQLIRTYYKNYVKKVLRRKN 187

Query: 181 TMSGLRYMDDPTIMGWELMNEPRCEVDSSGATVNRWVEEMGTYVKSIDKKHLVGIGMEGF 240
           TM+GLRYM+D TIMGWELMNE RC+VDSSG T+NRWVEEMG+YVKSIDK+HLVGIGMEGF
Sbjct: 188 TMNGLRYMEDATIMGWELMNEARCQVDSSGNTINRWVEEMGSYVKSIDKQHLVGIGMEGF 247

Query: 241 YGDSNPSRIKFNPNGYKVGTDFVTNNLIKVIDFATIHAYPDSWLPGQSEATKMAFLEEWM 300
           YGDS+P++IK NP  +K GTDFVTNNL K IDFATIH YPD+WLPG+SEAT+MAFLEEWM
Sbjct: 248 YGDSSPNKIKANPGSFKFGTDFVTNNLNKAIDFATIHVYPDAWLPGKSEATRMAFLEEWM 307

Query: 301 KIHWSDSKTILKKPLIFAEFGKSTKNQNQNQAFSVRDRDSFLTKVYSNIYNLARNGTTMA 360
            +HW DSK ILKKPLI  EFGKS +   QNQ FSVRD D+FL+KVYS IYNLAR G TMA
Sbjct: 308 ALHWMDSKNILKKPLILEEFGKSIR--GQNQTFSVRDSDAFLSKVYSIIYNLARKGATMA 367

Query: 361 GGLVWQVMAEGMESYYDGYEIVLSQNPLTTAIIMQQSKNMAAL 404
           GGLVWQVMAEGMESYYDGYEIVLSQNP T  II +QS  MAAL
Sbjct: 368 GGLVWQVMAEGMESYYDGYEIVLSQNPSTNTIITKQSNKMAAL 405

BLAST of Moc11g29270 vs. ExPASy Swiss-Prot
Match: Q9FZ03 (Mannan endo-1,4-beta-mannosidase 2 OS=Solanum lycopersicum OX=4081 GN=MAN2 PE=2 SV=2)

HSP 1 Score: 504.2 bits (1297), Expect = 1.4e-141
Identity = 233/377 (61.80%), Postives = 294/377 (77.98%), Query Frame = 0

Query: 29  FVGIGDGHFQLNGSPFVFNGFNSYWMMSVGADPNQRRKVSDAFRDAAAAGLTVCRTWAFA 88
           FV +   HF+LNGSPF+FNGFNSYW+M V A+P++R KVS+  R+A++AGL+VCRTWAF+
Sbjct: 39  FVRVNGAHFELNGSPFLFNGFNSYWLMHVAAEPSERYKVSEVLREASSAGLSVCRTWAFS 98

Query: 89  DGGYHALQISPGVYDESVFQALDFVIWEARKYGIWIILSLVNNYGDYGGRPQYVRWARAA 148
           DGG  ALQISPGVYDE VFQ LDFVI EA+KYGI +ILS VNNY D+GG+ QYV+WAR A
Sbjct: 99  DGGDRALQISPGVYDERVFQGLDFVISEAKKYGIRLILSFVNNYNDFGGKAQYVQWARNA 158

Query: 149 GAQIHNDDDFYTNQLIKGFYTGHVEKVLTRKNTMSGLRYMDDPTIMGWELMNEPRCEVDS 208
           GAQI+ DDDFYTN + K +Y  H++KV+TR NT++G+ Y DD TIM WELMNEPR + D 
Sbjct: 159 GAQINGDDDFYTNYITKNYYKNHIKKVVTRFNTITGMTYKDDSTIMAWELMNEPRNQADY 218

Query: 209 SGATVNRWVEEMGTYVKSIDKKHLVGIGMEGFYGDSNPSRIKFNPNGYKVGTDFVTNNLI 268
           SG T+N WV+EM ++VKS+D KHL+ IGMEGFYGDS P R   NP GY+VGTDF++N+LI
Sbjct: 219 SGNTLNAWVQEMASFVKSLDNKHLLEIGMEGFYGDSVPERKSINP-GYQVGTDFISNHLI 278

Query: 269 KVIDFATIHAYPDSWLPGQSEATKMAFLEEWMKIHWSDSKTILKKPLIFAEFGKSTKNQN 328
           K IDFATIHAY D WL GQS+  +M F+++WM  HW D+K ILKKPL+ AEFGKS+++  
Sbjct: 279 KEIDFATIHAYTDQWLSGQSDDAQMIFMQKWMTSHWQDAKNILKKPLVLAEFGKSSRDPG 338

Query: 329 QNQAFSVRDRDSFLTKVYSNIYNLARNGTTMAGGLVWQVMAEGMESYYDGYEIVLSQNPL 388
            NQ      RD+F++ +Y NIY+LA++G TM G L+WQ++A+GME+Y DGY I L +NP 
Sbjct: 339 YNQNI----RDTFMSTIYRNIYSLAKDGGTMGGSLIWQLVAQGMENYEDGYCIELGKNPS 398

Query: 389 TTAIIMQQSKNMAALQH 406
           T  II  QS  M AL H
Sbjct: 399 TAGIITSQSHAMTALAH 410

BLAST of Moc11g29270 vs. ExPASy Swiss-Prot
Match: Q6YM50 (Mannan endo-1,4-beta-mannosidase 5 OS=Solanum lycopersicum OX=4081 GN=MAN5 PE=2 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 1.4e-141
Identity = 233/377 (61.80%), Postives = 294/377 (77.98%), Query Frame = 0

Query: 29  FVGIGDGHFQLNGSPFVFNGFNSYWMMSVGADPNQRRKVSDAFRDAAAAGLTVCRTWAFA 88
           FV +   HF+LNGSPF+FNGFNSYW+M V A+P++R KVS+  R+A++AGL+VCRTWAF+
Sbjct: 39  FVRVNGAHFELNGSPFLFNGFNSYWLMHVAAEPSERYKVSEVLREASSAGLSVCRTWAFS 98

Query: 89  DGGYHALQISPGVYDESVFQALDFVIWEARKYGIWIILSLVNNYGDYGGRPQYVRWARAA 148
           DGG  ALQISPGVYDE VFQ LDFVI EA+KYGI +ILS VNNY D+GG+ QYV+WAR A
Sbjct: 99  DGGDRALQISPGVYDERVFQGLDFVISEAKKYGIRLILSFVNNYNDFGGKAQYVQWARNA 158

Query: 149 GAQIHNDDDFYTNQLIKGFYTGHVEKVLTRKNTMSGLRYMDDPTIMGWELMNEPRCEVDS 208
           GAQI+ DDDFYTN + K +Y  H++KV+TR NT++G+ Y DD TIM WELMNEPR + D 
Sbjct: 159 GAQINGDDDFYTNYITKNYYKNHIKKVVTRFNTITGMTYKDDSTIMAWELMNEPRNQADY 218

Query: 209 SGATVNRWVEEMGTYVKSIDKKHLVGIGMEGFYGDSNPSRIKFNPNGYKVGTDFVTNNLI 268
           SG T+N WV+EM ++VKS+D KHL+ IGMEGFYGDS P R   NP GY+VGTDF++N+LI
Sbjct: 219 SGNTLNAWVQEMASFVKSLDNKHLLEIGMEGFYGDSVPERKSINP-GYQVGTDFISNHLI 278

Query: 269 KVIDFATIHAYPDSWLPGQSEATKMAFLEEWMKIHWSDSKTILKKPLIFAEFGKSTKNQN 328
           K IDFATIHAY D WL GQS+  +M F+++WM  HW D+K ILKKPL+ AEFGKS+++  
Sbjct: 279 KEIDFATIHAYTDQWLSGQSDDAQMIFMQKWMTSHWQDAKNILKKPLVLAEFGKSSRDPG 338

Query: 329 QNQAFSVRDRDSFLTKVYSNIYNLARNGTTMAGGLVWQVMAEGMESYYDGYEIVLSQNPL 388
            NQ      RD+F++ +Y NIY+LA++G TM G L+WQ++A+GME+Y DGY I L +NP 
Sbjct: 339 YNQNI----RDTFMSTIYRNIYSLAKDGGTMGGSLIWQLVAQGMENYEDGYCIELGKNPS 398

Query: 389 TTAIIMQQSKNMAALQH 406
           T  II  QS  M AL H
Sbjct: 399 TAGIITSQSHAMTALAH 410

BLAST of Moc11g29270 vs. ExPASy Swiss-Prot
Match: O48540 (Mannan endo-1,4-beta-mannosidase 1 OS=Solanum lycopersicum OX=4081 GN=MAN1 PE=1 SV=2)

HSP 1 Score: 491.5 bits (1264), Expect = 9.1e-138
Identity = 228/379 (60.16%), Postives = 289/379 (76.25%), Query Frame = 0

Query: 25  APAAFVGIGDGHFQLNGSPFVFNGFNSYWMMSVGADPNQRRKVSDAFRDAAAAGLTVCRT 84
           A + F+G+ D HF+LNGSPF+FNGFNSYW+M V ADP +R KV++  +DA+ AGL+VCRT
Sbjct: 23  ANSGFIGVKDSHFELNGSPFLFNGFNSYWLMHVAADPTERYKVTEVLKDASVAGLSVCRT 82

Query: 85  WAFADGGYHALQISPGVYDESVFQALDFVIWEARKYGIWIILSLVNNYGDYGGRPQYVRW 144
           WAF+DGG  ALQISPG+YDE VFQ LDFVI EA+KYGI +ILS VN + D+GG+ QYV W
Sbjct: 83  WAFSDGGDRALQISPGIYDERVFQGLDFVIAEAKKYGIRLILSFVNQWNDFGGKAQYVWW 142

Query: 145 ARAAGAQIHNDDDFYTNQLIKGFYTGHVEKVLTRKNTMSGLRYMDDPTIMGWELMNEPRC 204
           AR AGAQI NDD+FYT+ ++K +   H+EKV+TR N+++ + Y DDPTIM WELMNEPR 
Sbjct: 143 ARNAGAQISNDDEFYTHPMLKKYLKNHIEKVVTRLNSITKVAYKDDPTIMAWELMNEPRD 202

Query: 205 EVDSSGATVNRWVEEMGTYVKSIDKKHLVGIGMEGFYGDSNPSRIKFNPNGYKVGTDFVT 264
           + D SG TVN WV+EM ++VKS+D KHL+ +GMEGFYGDS P R   NP GY+VGTDF++
Sbjct: 203 QADYSGKTVNGWVQEMASFVKSLDNKHLLEVGMEGFYGDSIPERKSVNP-GYQVGTDFIS 262

Query: 265 NNLIKVIDFATIHAYPDSWLPGQSEATKMAFLEEWMKIHWSDSKTILKKPLIFAEFGKST 324
           N+LI  IDFATIHAY D W+ GQS+  ++ ++E+W+  HW D++ ILKKPL+ AEFGKS+
Sbjct: 263 NHLINEIDFATIHAYTDQWVSGQSDDAQLVWMEKWITSHWEDARNILKKPLVLAEFGKSS 322

Query: 325 KNQNQNQAFSVRDRDSFLTKVYSNIYNLARNGTTMAGGLVWQVMAEGMESYYDGYEIVLS 384
           + Q          RD F++ VY N+YNLA+ G TMAG LVWQ+MA GME+Y DGY IVL 
Sbjct: 323 RGQG--------SRDIFMSSVYRNVYNLAKEGGTMAGSLVWQLMAHGMENYDDGYCIVLG 382

Query: 385 QNPLTTAIIMQQSKNMAAL 404
           Q P TT II  Q+  M AL
Sbjct: 383 QTPSTTQIISDQAHVMTAL 392

BLAST of Moc11g29270 vs. ExPASy Swiss-Prot
Match: Q9FUQ6 (Mannan endo-1,4-beta-mannosidase 3 OS=Solanum lycopersicum OX=4081 GN=MAN3 PE=3 SV=1)

HSP 1 Score: 477.2 bits (1227), Expect = 1.8e-133
Identity = 230/405 (56.79%), Postives = 298/405 (73.58%), Query Frame = 0

Query: 2   RWLVWGVLLVLLGGHSSSSSPAPAPAAFVGIGDGHFQLNGSPFVFNGFNSYWMMSVGADP 61
           R  + G+ L+LL      +    A + F+G+ D HF+LNGSPF+FNGFNSYW+M V ADP
Sbjct: 7   RSCISGLFLLLL------ALSCEANSGFIGVKDSHFELNGSPFLFNGFNSYWLMHVAADP 66

Query: 62  NQRRKVSDAFRDAAAAGLTVCRTWAFADGGYHALQISPGVYDESVFQALDFVIWEARKYG 121
            +R KV++  +DA+ AGL+VCRTWAF+DGG  ALQISPGVYDE VFQ LDFVI EA+KYG
Sbjct: 67  AERYKVTEVLKDASTAGLSVCRTWAFSDGGDRALQISPGVYDERVFQGLDFVIAEAKKYG 126

Query: 122 IWIILSLVNNYGDYGGRPQYVRWARAAGAQIHNDDDFYTNQLIKGFYTGHVEKVLTRKNT 181
           + +ILS VN + D+GG+ +YV WAR AG QI NDD+FYT+ ++K +   H+E V+TR N+
Sbjct: 127 VRLILSFVNQWNDFGGKAEYVWWARNAGVQISNDDEFYTHPILKKYLKNHIE-VVTRLNS 186

Query: 182 MSGLRYMDDPTIMGWELMNEPRCEVDSSGATVN-RWVEEMGTYVKSIDKKHLVGIGMEGF 241
           ++ + Y DD TIM WELMNEPR + D SG TVN  WV+EM ++VKS+D KHL+ +GMEGF
Sbjct: 187 ITKVAYKDDATIMAWELMNEPRDQADYSGKTVNVGWVQEMASFVKSLDNKHLLEVGMEGF 246

Query: 242 YGDSNPSRIKFNPNGYKVGTDFVTNNLIKVIDFATIHAYPDSWLPGQSEATKMAFLEEWM 301
           YGDS P R   NP GY+VGTDF++N+LI  IDFATIHAY D WL GQS+  ++A++E+W+
Sbjct: 247 YGDSIPERKLVNP-GYQVGTDFISNHLINEIDFATIHAYTDQWLSGQSDEAQLAWMEKWI 306

Query: 302 KIHWSDSKTILKKPLIFAEFGKSTKNQNQNQAFSVRDRDSFLTKVYSNIYNLARNGTTMA 361
           + HW D++ ILKKPL+ AEFGKS+++   +       RD F++ VY N+YNLA+ G TM 
Sbjct: 307 RSHWEDARNILKKPLVLAEFGKSSRSGEGS-------RDIFMSSVYRNVYNLAKEGGTMG 366

Query: 362 GGLVWQVMAEGMESYYDGYEIVLSQNPLTTAIIMQQSKNMAALQH 406
           G LVWQ+MA GME+Y DGY IVL  NP TT II  Q+  M AL H
Sbjct: 367 GSLVWQLMAHGMENYDDGYSIVLGLNPSTTQIISNQAHIMTALAH 396

BLAST of Moc11g29270 vs. ExPASy Swiss-Prot
Match: Q0JKM9 (Mannan endo-1,4-beta-mannosidase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=MAN1 PE=2 SV=2)

HSP 1 Score: 433.0 bits (1112), Expect = 3.9e-120
Identity = 201/377 (53.32%), Postives = 269/377 (71.35%), Query Frame = 0

Query: 29  FVGIGDGHFQLNGSPFVFNGFNSYWMMSVGADPNQRRKVSDAFRDAAAAGLTVCRTWAFA 88
           FV      F L+G+P+  NGFN+YW+M + ADP+QR KVS A  +AA  GLTV RTWAF+
Sbjct: 37  FVRAQGTRFVLDGNPYYANGFNAYWLMLLAADPSQRGKVSAALGEAAGHGLTVARTWAFS 96

Query: 89  D-GGYHALQISPGVYDESVFQALDFVIWEARKYGIWIILSLVNNYGDYGGRPQYVRWARA 148
           D GG +ALQ+SPG Y+E+ F+ LDFV+ EARKYGI +ILSLV+NY  +GGR QYV WARA
Sbjct: 97  DGGGGNALQLSPGNYNENTFKGLDFVLSEARKYGIKVILSLVDNYDSFGGRKQYVNWARA 156

Query: 149 AGAQIHNDDDFYTNQLIKGFYTGHVEKVLTRKNTMSGLRYMDDPTIMGWELMNEPRCEVD 208
            G  I +DD+F+TN ++KGFY  HV+ VLTRKNT++G+ Y DDPTI+ WELMNEPRC+ D
Sbjct: 157 QGQGIGSDDEFFTNPVVKGFYKNHVKTVLTRKNTITGVAYRDDPTILAWELMNEPRCQSD 216

Query: 209 SSGATVNRWVEEMGTYVKSIDKKHLVGIGMEGFYGDSNPSRI-KFNPNGYKVGTDFVTNN 268
            SG TV  W+ EM  +VKSID+ H++ +G+EGFYG S+PSRI   NP+GY++GTDF+ NN
Sbjct: 217 LSGRTVQSWITEMAAHVKSIDRNHMLEVGLEGFYGASSPSRIAAVNPSGYQLGTDFIANN 276

Query: 269 LIKVIDFATIHAYPDSWLPGQSEATKMAFLEEWMKIHWSDSKTILKKPLIFAEFGKSTKN 328
            +  IDFAT+H+YPD WL G+ +  ++ F+  W+  H +D++ +L+KPL+ AEFGKS K 
Sbjct: 277 QVPGIDFATVHSYPDQWLSGKDDQAQLGFMGRWLDAHIADAQAVLRKPLLIAEFGKSWK- 336

Query: 329 QNQNQAFSVRDRDSFLTKVYSNIYNLARNGTTMAGGLVWQVMAEGMESYYDGYEIVLSQN 388
              +  +S   RD+    VY+ IY  AR G    GGL WQ++  GM+SY DGYE+V  + 
Sbjct: 337 ---DPGYSSGQRDALYGTVYAKIYESARRGGATVGGLFWQLLVPGMDSYRDGYEVVFGET 396

Query: 389 PLTTAIIMQQSKNMAAL 404
           P TT +I   S+ +  L
Sbjct: 397 PSTTGVITTNSRRLRFL 409

BLAST of Moc11g29270 vs. ExPASy TrEMBL
Match: A0A6J1CK63 (Mannan endo-1,4-beta-mannosidase OS=Momordica charantia OX=3673 GN=LOC111012035 PE=3 SV=1)

HSP 1 Score: 845.5 bits (2183), Expect = 9.2e-242
Identity = 405/405 (100.00%), Postives = 405/405 (100.00%), Query Frame = 0

Query: 1   MRWLVWGVLLVLLGGHSSSSSPAPAPAAFVGIGDGHFQLNGSPFVFNGFNSYWMMSVGAD 60
           MRWLVWGVLLVLLGGHSSSSSPAPAPAAFVGIGDGHFQLNGSPFVFNGFNSYWMMSVGAD
Sbjct: 1   MRWLVWGVLLVLLGGHSSSSSPAPAPAAFVGIGDGHFQLNGSPFVFNGFNSYWMMSVGAD 60

Query: 61  PNQRRKVSDAFRDAAAAGLTVCRTWAFADGGYHALQISPGVYDESVFQALDFVIWEARKY 120
           PNQRRKVSDAFRDAAAAGLTVCRTWAFADGGYHALQISPGVYDESVFQALDFVIWEARKY
Sbjct: 61  PNQRRKVSDAFRDAAAAGLTVCRTWAFADGGYHALQISPGVYDESVFQALDFVIWEARKY 120

Query: 121 GIWIILSLVNNYGDYGGRPQYVRWARAAGAQIHNDDDFYTNQLIKGFYTGHVEKVLTRKN 180
           GIWIILSLVNNYGDYGGRPQYVRWARAAGAQIHNDDDFYTNQLIKGFYTGHVEKVLTRKN
Sbjct: 121 GIWIILSLVNNYGDYGGRPQYVRWARAAGAQIHNDDDFYTNQLIKGFYTGHVEKVLTRKN 180

Query: 181 TMSGLRYMDDPTIMGWELMNEPRCEVDSSGATVNRWVEEMGTYVKSIDKKHLVGIGMEGF 240
           TMSGLRYMDDPTIMGWELMNEPRCEVDSSGATVNRWVEEMGTYVKSIDKKHLVGIGMEGF
Sbjct: 181 TMSGLRYMDDPTIMGWELMNEPRCEVDSSGATVNRWVEEMGTYVKSIDKKHLVGIGMEGF 240

Query: 241 YGDSNPSRIKFNPNGYKVGTDFVTNNLIKVIDFATIHAYPDSWLPGQSEATKMAFLEEWM 300
           YGDSNPSRIKFNPNGYKVGTDFVTNNLIKVIDFATIHAYPDSWLPGQSEATKMAFLEEWM
Sbjct: 241 YGDSNPSRIKFNPNGYKVGTDFVTNNLIKVIDFATIHAYPDSWLPGQSEATKMAFLEEWM 300

Query: 301 KIHWSDSKTILKKPLIFAEFGKSTKNQNQNQAFSVRDRDSFLTKVYSNIYNLARNGTTMA 360
           KIHWSDSKTILKKPLIFAEFGKSTKNQNQNQAFSVRDRDSFLTKVYSNIYNLARNGTTMA
Sbjct: 301 KIHWSDSKTILKKPLIFAEFGKSTKNQNQNQAFSVRDRDSFLTKVYSNIYNLARNGTTMA 360

Query: 361 GGLVWQVMAEGMESYYDGYEIVLSQNPLTTAIIMQQSKNMAALQH 406
           GGLVWQVMAEGMESYYDGYEIVLSQNPLTTAIIMQQSKNMAALQH
Sbjct: 361 GGLVWQVMAEGMESYYDGYEIVLSQNPLTTAIIMQQSKNMAALQH 405

BLAST of Moc11g29270 vs. ExPASy TrEMBL
Match: A0A5D3BKZ3 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G00890 PE=3 SV=1)

HSP 1 Score: 665.2 bits (1715), Expect = 1.7e-187
Identity = 315/404 (77.97%), Postives = 355/404 (87.87%), Query Frame = 0

Query: 1   MRWLVWGVLLVLLGGHSSSSSPAPAPAAFVGIGDGHFQLNGSPFVFNGFNSYWMMSVGAD 60
           +RWL+WG+LL++    + SSS +P  A FVG+ DGHFQLNGSPF+FNGFNSYWMMSV AD
Sbjct: 8   IRWLIWGLLLLI---STLSSSSSPVTAGFVGVEDGHFQLNGSPFLFNGFNSYWMMSVAAD 67

Query: 61  PNQRRKVSDAFRDAAAAGLTVCRTWAFADGGYHALQISPGVYDESVFQALDFVIWEARKY 120
           PNQR KVS  FRDAA AGLTVCRTWAF DGG+HALQISPGVYDESVFQ LDFVI+EARKY
Sbjct: 68  PNQRHKVSQVFRDAATAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIFEARKY 127

Query: 121 GIWIILSLVNNYGDYGGRPQYVRWARAAGAQIHNDDDFYTNQLIKGFYTGHVEKVLTRKN 180
           GI +ILSLVNN+ DYGGR  YVRWA AAG Q+ N+DDFYTNQLI+ +Y  HV+KVL RKN
Sbjct: 128 GIRMILSLVNNFKDYGGRAAYVRWAEAAGVQVQNEDDFYTNQLIRTYYKNHVQKVLRRKN 187

Query: 181 TMSGLRYMDDPTIMGWELMNEPRCEVDSSGATVNRWVEEMGTYVKSIDKKHLVGIGMEGF 240
           TMS LRYMDDPTIMGWELMNEPRC+VDSSG TVNRWVEEMG+YVKSID++HLVGIGMEGF
Sbjct: 188 TMSCLRYMDDPTIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDRQHLVGIGMEGF 247

Query: 241 YGDSNPSRIKFNPNGYKVGTDFVTNNLIKVIDFATIHAYPDSWLPGQSEATKMAFLEEWM 300
           YGDS P++IK NP+ YKVGTDF+TNNL K ID+ATIHAYPD+WLPG+SE TKMAFLEEW+
Sbjct: 248 YGDSIPTKIKANPSSYKVGTDFITNNLNKAIDYATIHAYPDAWLPGKSETTKMAFLEEWV 307

Query: 301 KIHWSDSKTILKKPLIFAEFGKSTKNQNQNQAFSVRDRDSFLTKVYSNIYNLARNGTTMA 360
            +HW+DSKTILKKPLIF EFG+S +  +QNQ +SVRDRD+FL+KVYS IYNLA+NG TMA
Sbjct: 308 ALHWTDSKTILKKPLIFEEFGRSIR--DQNQTYSVRDRDAFLSKVYSIIYNLAKNGATMA 367

Query: 361 GGLVWQVMAEGMESYYDGYEIVLSQNPLTTAIIMQQSKNMAALQ 405
           GGLVWQVMAEGMESYYDGYEIVLSQNP T AII QQS  MAAL+
Sbjct: 368 GGLVWQVMAEGMESYYDGYEIVLSQNPSTNAIITQQSNKMAALK 406

BLAST of Moc11g29270 vs. ExPASy TrEMBL
Match: A0A1S3CB04 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103498662 PE=3 SV=1)

HSP 1 Score: 665.2 bits (1715), Expect = 1.7e-187
Identity = 315/404 (77.97%), Postives = 355/404 (87.87%), Query Frame = 0

Query: 1   MRWLVWGVLLVLLGGHSSSSSPAPAPAAFVGIGDGHFQLNGSPFVFNGFNSYWMMSVGAD 60
           +RWL+WG+LL++    + SSS +P  A FVG+ DGHFQLNGSPF+FNGFNSYWMMSV AD
Sbjct: 8   IRWLIWGLLLLI---STLSSSSSPVTAGFVGVEDGHFQLNGSPFLFNGFNSYWMMSVAAD 67

Query: 61  PNQRRKVSDAFRDAAAAGLTVCRTWAFADGGYHALQISPGVYDESVFQALDFVIWEARKY 120
           PNQR KVS  FRDAA AGLTVCRTWAF DGG+HALQISPGVYDESVFQ LDFVI+EARKY
Sbjct: 68  PNQRHKVSQVFRDAATAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIFEARKY 127

Query: 121 GIWIILSLVNNYGDYGGRPQYVRWARAAGAQIHNDDDFYTNQLIKGFYTGHVEKVLTRKN 180
           GI +ILSLVNN+ DYGGR  YVRWA AAG Q+ N+DDFYTNQLI+ +Y  HV+KVL RKN
Sbjct: 128 GIRMILSLVNNFKDYGGRAAYVRWAEAAGVQVQNEDDFYTNQLIRTYYKNHVQKVLRRKN 187

Query: 181 TMSGLRYMDDPTIMGWELMNEPRCEVDSSGATVNRWVEEMGTYVKSIDKKHLVGIGMEGF 240
           TMS LRYMDDPTIMGWELMNEPRC+VDSSG TVNRWVEEMG+YVKSID++HLVGIGMEGF
Sbjct: 188 TMSCLRYMDDPTIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDRQHLVGIGMEGF 247

Query: 241 YGDSNPSRIKFNPNGYKVGTDFVTNNLIKVIDFATIHAYPDSWLPGQSEATKMAFLEEWM 300
           YGDS P++IK NP+ YKVGTDF+TNNL K ID+ATIHAYPD+WLPG+SE TKMAFLEEW+
Sbjct: 248 YGDSIPTKIKANPSSYKVGTDFITNNLNKAIDYATIHAYPDAWLPGKSETTKMAFLEEWV 307

Query: 301 KIHWSDSKTILKKPLIFAEFGKSTKNQNQNQAFSVRDRDSFLTKVYSNIYNLARNGTTMA 360
            +HW+DSKTILKKPLIF EFG+S +  +QNQ +SVRDRD+FL+KVYS IYNLA+NG TMA
Sbjct: 308 ALHWTDSKTILKKPLIFEEFGRSIR--DQNQTYSVRDRDAFLSKVYSIIYNLAKNGATMA 367

Query: 361 GGLVWQVMAEGMESYYDGYEIVLSQNPLTTAIIMQQSKNMAALQ 405
           GGLVWQVMAEGMESYYDGYEIVLSQNP T AII QQS  MAAL+
Sbjct: 368 GGLVWQVMAEGMESYYDGYEIVLSQNPSTNAIITQQSNKMAALK 406

BLAST of Moc11g29270 vs. ExPASy TrEMBL
Match: A0A0A0KSS2 (Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_5G648710 PE=3 SV=1)

HSP 1 Score: 663.7 bits (1711), Expect = 5.0e-187
Identity = 314/403 (77.92%), Postives = 354/403 (87.84%), Query Frame = 0

Query: 1   MRWLVWGVLLVLLGGHSSSSSPAPAPAAFVGIGDGHFQLNGSPFVFNGFNSYWMMSVGAD 60
           MRWL+W +  ++     S+SS +PAPAAFVG+ DGHFQLNGSPF+FNGFNSYWMMSV  D
Sbjct: 9   MRWLIWALFFLI--STLSASSSSPAPAAFVGVQDGHFQLNGSPFLFNGFNSYWMMSVATD 68

Query: 61  PNQRRKVSDAFRDAAAAGLTVCRTWAFADGGYHALQISPGVYDESVFQALDFVIWEARKY 120
           PNQR KV+  FRDAAAAGLTVCRTWAF DGG+HALQISPGVYDESVFQ LDFVI+EARKY
Sbjct: 69  PNQRNKVTQVFRDAAAAGLTVCRTWAFNDGGFHALQISPGVYDESVFQGLDFVIFEARKY 128

Query: 121 GIWIILSLVNNYGDYGGRPQYVRWARAAGAQIHNDDDFYTNQLIKGFYTGHVEKVLTRKN 180
           GI +ILSLVNN+ DYGGR  YVRWA AAG Q+H++DDFYTNQLIK +Y  HV+KVL+RKN
Sbjct: 129 GIRMILSLVNNFKDYGGRAAYVRWAEAAGVQVHDEDDFYTNQLIKTYYKNHVQKVLSRKN 188

Query: 181 TMSGLRYMDDPTIMGWELMNEPRCEVDSSGATVNRWVEEMGTYVKSIDKKHLVGIGMEGF 240
           TM+GL YM+D TIMGWELMNEPRC+VDSSG TVNRWVEEMG+YVKSIDK+HLVGIGMEGF
Sbjct: 189 TMNGLIYMEDATIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDKQHLVGIGMEGF 248

Query: 241 YGDSNPSRIKFNPNGYKVGTDFVTNNLIKVIDFATIHAYPDSWLPGQSEATKMAFLEEWM 300
           YGDS+P++IK NP  +K GTDF+TNNL K IDFATIHAYPD+WLPG+SE TKMAFLEEW+
Sbjct: 249 YGDSSPNKIKANPGSFKFGTDFITNNLNKAIDFATIHAYPDAWLPGKSEGTKMAFLEEWI 308

Query: 301 KIHWSDSKTILKKPLIFAEFGKSTKNQNQNQAFSVRDRDSFLTKVYSNIYNLARNGTTMA 360
            +HW+DSKTILKKPLIF EFGKS ++QNQ  + SVRDRD+FL+KV+S IYNLARNG TMA
Sbjct: 309 ALHWTDSKTILKKPLIFEEFGKSIRDQNQTSS-SVRDRDAFLSKVFSIIYNLARNGATMA 368

Query: 361 GGLVWQVMAEGMESYYDGYEIVLSQNPLTTAIIMQQSKNMAAL 404
           GGLVWQVMAEGMESYYDGYEIVLSQ P TTAII  QS NMAAL
Sbjct: 369 GGLVWQVMAEGMESYYDGYEIVLSQTPSTTAIITHQSNNMAAL 408

BLAST of Moc11g29270 vs. ExPASy TrEMBL
Match: A0A5A7TCI2 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold64G00910 PE=3 SV=1)

HSP 1 Score: 593.2 bits (1528), Expect = 8.2e-166
Identity = 289/404 (71.53%), Postives = 328/404 (81.19%), Query Frame = 0

Query: 1   MRWLVWGVLLVLLGGHSSSSSPAPAPAAFVGIGDGHFQLNGSPFVFNGFNSYWMMSVGAD 60
           +RWL+WG+LL++    + SSS +P  A FVG+ DGHFQLNGSPF+FNGFNSYWMMSV AD
Sbjct: 8   IRWLIWGLLLLI---STLSSSSSPVTAGFVGVEDGHFQLNGSPFLFNGFNSYWMMSVAAD 67

Query: 61  PNQRRKVSDAFRDAAAAGLTVCRTWAFADGGYHALQISPGVYDESVFQALDFVIWEARKY 120
           PNQR KVS  FRDAA AGLT                             LDFVI+EARKY
Sbjct: 68  PNQRHKVSQVFRDAATAGLT----------------------------GLDFVIFEARKY 127

Query: 121 GIWIILSLVNNYGDYGGRPQYVRWARAAGAQIHNDDDFYTNQLIKGFYTGHVEKVLTRKN 180
           GI +ILSLVNN+ DYGGR  YVRWA AAG Q+ N+DDFYTNQLI+ +Y  HV+KVL RKN
Sbjct: 128 GIRMILSLVNNFKDYGGRAAYVRWAEAAGVQVQNEDDFYTNQLIRTYYKNHVQKVLRRKN 187

Query: 181 TMSGLRYMDDPTIMGWELMNEPRCEVDSSGATVNRWVEEMGTYVKSIDKKHLVGIGMEGF 240
           TMS LRYMDDPTIMGWELMNEPRC+VDSSG TVNRWVEEMG+YVKSID++HLVGIGMEGF
Sbjct: 188 TMSCLRYMDDPTIMGWELMNEPRCQVDSSGNTVNRWVEEMGSYVKSIDRQHLVGIGMEGF 247

Query: 241 YGDSNPSRIKFNPNGYKVGTDFVTNNLIKVIDFATIHAYPDSWLPGQSEATKMAFLEEWM 300
           YGDS P++IK NP+ YKVGTDF+TNNL K ID+ATIHAYPD+WLPG+SE TKMAFLEEW+
Sbjct: 248 YGDSIPTKIKANPSSYKVGTDFITNNLNKAIDYATIHAYPDAWLPGKSETTKMAFLEEWV 307

Query: 301 KIHWSDSKTILKKPLIFAEFGKSTKNQNQNQAFSVRDRDSFLTKVYSNIYNLARNGTTMA 360
            +HW+DSKTILKKPLIF EFG+S +  +QNQ +SVRDRD+FL+KVYS IYNLA+NG TMA
Sbjct: 308 ALHWTDSKTILKKPLIFEEFGRSIR--DQNQTYSVRDRDAFLSKVYSIIYNLAKNGATMA 367

Query: 361 GGLVWQVMAEGMESYYDGYEIVLSQNPLTTAIIMQQSKNMAALQ 405
           GGLVWQVMAEGMESYYDGYEIVLSQNP T AII QQS  MAAL+
Sbjct: 368 GGLVWQVMAEGMESYYDGYEIVLSQNPSTNAIITQQSNKMAALK 378

BLAST of Moc11g29270 vs. TAIR 10
Match: AT5G66460.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 413.3 bits (1061), Expect = 2.2e-115
Identity = 191/376 (50.80%), Postives = 251/376 (66.76%), Query Frame = 0

Query: 29  FVGIGDGHFQLNGSPFVFNGFNSYWMMSVGADPNQRRKVSDAFRDAAAAGLTVCRTWAFA 88
           FV      F LNG P+  NGFN+YW+M V +DP+QR K+S AF+DA+  GLTV RTWAF+
Sbjct: 31  FVRTKGVQFSLNGYPYYANGFNAYWLMYVASDPSQRSKISTAFQDASRHGLTVARTWAFS 90

Query: 89  DGGYHALQISPGVYDESVFQALDFVIWEARKYGIWIILSLVNNYGDYGGRPQYVRWARAA 148
           DGGY ALQ SPG Y+E +FQ LDF + EAR++GI IILS  NNY  +GGR QYV WAR+ 
Sbjct: 91  DGGYRALQYSPGSYNEDMFQGLDFALAEARRHGIKIILSFANNYESFGGRKQYVDWARSR 150

Query: 149 GAQIHNDDDFYTNQLIKGFYTGHVEKVLTRKNTMSGLRYMDDPTIMGWELMNEPRCEVDS 208
           G  + ++DDF+T+ L+K FY  H++ VL R NT + + Y DDPTIM WELMNEPRC  D 
Sbjct: 151 GRPVSSEDDFFTDSLVKDFYKNHIKAVLNRFNTFTKVHYKDDPTIMAWELMNEPRCPSDP 210

Query: 209 SGATVNRWVEEMGTYVKSIDKKHLVGIGMEGFYGDSNPSRIKFNPNGYKVGTDFVTNNLI 268
           SG  +  W+ EM  +VKS+D+ HL+  G+EGFYG S+P     NP G + GTDF+ NN I
Sbjct: 211 SGRAIQAWITEMAAHVKSLDRNHLLEAGLEGFYGQSSPQSKTLNPPG-QFGTDFIANNRI 270

Query: 269 KVIDFATIHAYPDSWLPGQSEATKMAFLEEWMKIHWSDSKTILKKPLIFAEFGKSTKNQN 328
             IDF T+H+YPD W P  SE ++M FL +W+  H  D++ +L KP+I AEFGKS K   
Sbjct: 271 PGIDFVTVHSYPDEWFPDSSEQSQMDFLNKWLDAHIQDAQNVLHKPIILAEFGKSMKKPG 330

Query: 329 QNQAFSVRDRDSFLTKVYSNIYNLARNGTTMAGGLVWQVMAEGMESYYDGYEIVLSQNPL 388
              A     RD     VYS IY  A+ G   AGGL WQ++  G++++ DGY I+LSQ+  
Sbjct: 331 YTPA----QRDIVFNTVYSKIYGSAKRGGAAAGGLFWQLLVNGIDNFQDGYGIILSQSSS 390

Query: 389 TTAIIMQQSKNMAALQ 405
           T  +I QQS+ +  ++
Sbjct: 391 TVNVISQQSRKLTLIR 401

BLAST of Moc11g29270 vs. TAIR 10
Match: AT3G10890.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 388.7 bits (997), Expect = 5.9e-108
Identity = 188/408 (46.08%), Postives = 254/408 (62.25%), Query Frame = 0

Query: 1   MRWLVWGVLLVLLGGHSSSSSPAPAPAA--FVGIGDGHFQLNGSPFVFNGFNSYWMMSVG 60
           M+ L + VLL +L   +SS     + ++  FV      F LNG PF  NGFN+YW+    
Sbjct: 1   MKCLCFVVLLAILIAQNSSDLGVKSASSDGFVSRKGVQFILNGKPFYANGFNAYWLAYEA 60

Query: 61  ADPNQRRKVSDAFRDAAAAGLTVCRTWAFADGGYHALQISPGVYDESVFQALDFVIWEAR 120
            D   R K++  F++A    LT+ RTW F DGGY ALQI+PGVYDE  FQ LDF I EA+
Sbjct: 61  TDSTTRFKITYVFQNATIHDLTIVRTWGFRDGGYRALQIAPGVYDEKTFQGLDFAIAEAK 120

Query: 121 KYGIWIILSLVNNYGDYGGRPQYVRWARAAGAQIHNDDDFYTNQLIKGFYTGHVEKVLTR 180
           + GI +I++ VNNY D+GGR QYV WA+  G  + +DDDFYTN L+K +Y  HV+ ++ R
Sbjct: 121 RLGIKMIITFVNNYSDFGGRKQYVDWAKNTGQNVSSDDDFYTNPLVKQYYKNHVKTMVNR 180

Query: 181 KNTMSGLRYMDDPTIMGWELMNEPRCEVDSSGATVNRWVEEMGTYVKSIDKKHLVGIGME 240
            NT + + Y D+PTIMGWELMNEP+C  D SG T+  W+ EM  YVKS+D KHL+  G+E
Sbjct: 181 VNTFTKVEYKDEPTIMGWELMNEPQCRADPSGKTLTAWMNEMALYVKSVDSKHLLSTGLE 240

Query: 241 GFYGDSNPSR-IKFNPNGYKV-GTDFVTNNLIKVIDFATIHAYPDSWLPGQSEATKMAFL 300
           GFYGDS+P R    NP    V GTDF+ N+ +  IDFA+IH+YPD W P   E +++  L
Sbjct: 241 GFYGDSSPQRKTSLNPVAANVLGTDFIANHKLDAIDFASIHSYPDLWFPNLDEKSRLNLL 300

Query: 301 EEWMKIHWSDSKTILKKPLIFAEFGKSTKNQNQNQAFSVRDRDSFLTKVYSNIYNLARNG 360
            +W++ H  D++ ILKKPLI  EFGK T      QA     RD+     +  IY  A  G
Sbjct: 301 RKWLECHLEDAQNILKKPLILGEFGKPTNTPGYTQA----QRDAVFNATFDTIYESAEKG 360

Query: 361 TTMAGGLVWQVMAEGMESYYDGYEIVLSQNPLTTAIIMQQSKNMAALQ 405
              AG L W V+++GM ++ D   IVLS+N  T  II ++S+ +  ++
Sbjct: 361 GPAAGALFWHVISDGMNNFKDPLSIVLSENSTTVNIITEESRKLGLIR 404

BLAST of Moc11g29270 vs. TAIR 10
Match: AT5G01930.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 387.5 bits (994), Expect = 1.3e-107
Identity = 186/367 (50.68%), Postives = 240/367 (65.40%), Query Frame = 0

Query: 37  FQLNGSPFVFNGFNSYWMMSVGADPNQRRKVSDAFRDAAAAGLTVCRTWAFADGGYHALQ 96
           F LNG PF  NGFN+YWMM++ AD + R KV++ F+ A+A G+TV RTWAF DG + ALQ
Sbjct: 55  FTLNGQPFYVNGFNTYWMMTLAADNSTRGKVTEVFQQASAVGMTVGRTWAFNDGQWRALQ 114

Query: 97  ISPGVYDESVFQALDFVIWEARKYGIWIILSLVNNYGDYGGRPQYVRWARAAGAQIHNDD 156
            SP VYDE VF+ALDFV+ EARKY I +ILSLVNN+  YGG+ QYV+W  A+G  + +DD
Sbjct: 115 KSPSVYDEEVFKALDFVLSEARKYKIRLILSLVNNWDAYGGKAQYVKWGNASGLNLTSDD 174

Query: 157 DFYTNQLIKGFYTGHVEKVLTRKNTMSGLRYMDDPTIMGWELMNEPRCEVDSSGATVNRW 216
           DF+TN  ++ FY  HV  VL R NT + + Y +DPTI  WELMNEPRC  D SG  +  W
Sbjct: 175 DFFTNPTLRNFYQSHVRTVLNRVNTFTNITYKNDPTIFAWELMNEPRCPSDPSGDKLQSW 234

Query: 217 VEEMGTYVKSIDKKHLVGIGMEGFYGDSNPSRIKFNPNGY--KVGTDFVTNNLIKVIDFA 276
           ++EM  +VKS+D KHLV IG+EGFYG S P+R +FNPN Y  +VGTDF+ NN +  IDFA
Sbjct: 235 IQEMAVFVKSLDAKHLVEIGLEGFYGPSAPARTRFNPNPYAAQVGTDFIRNNQVLGIDFA 294

Query: 277 TIHAYPDSWLPGQSEATKMAFLEEWMKIHWSDSKTILKKPLIFAEFGKSTKNQNQNQAFS 336
           ++H YPDSW+      + + F   WM+ H  D++  L  P++F EFG S  +   N +F 
Sbjct: 295 SVHVYPDSWISPAVSNSFLEFTSSWMQAHVEDAEMYLGMPVLFTEFGVSAHDPGFNTSF- 354

Query: 337 VRDRDSFLTKVYSNIYNLARNGTTMAGGLVWQVMAEGMESYYDGYEIVLSQNPLTTAIIM 396
              RD  L  VY    N  R G   AG LVWQV  +G E   DGY + L++    + II 
Sbjct: 355 ---RDMMLNTVYKMTLNSTRKGGAGAGSLVWQVFPQGAEFMDDGYAVYLTRAHTASKIIS 414

Query: 397 QQSKNMA 402
            QSK +A
Sbjct: 415 LQSKRLA 417

BLAST of Moc11g29270 vs. TAIR 10
Match: AT1G02310.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 364.4 bits (934), Expect = 1.2e-100
Identity = 186/383 (48.56%), Postives = 238/383 (62.14%), Query Frame = 0

Query: 29  FVGIGDGHFQLNGSPFVFNGFNSYWMMSVGAD--PNQRRKVSDAFRDAAAAGLTVCRTWA 88
           FVG     F LNG     NGFN+YWMM+  AD     R  V+ A R A+A G+ V R W 
Sbjct: 29  FVGRNGTQFVLNGEQVYLNGFNAYWMMTTAADTASKGRATVTTALRQASAVGMNVARIWG 88

Query: 89  FADGGYHALQISPGVYDESVFQALDFVIWEARKYGIWIILSLVNNYGDYGGRPQYVRWAR 148
           F +G Y  LQISPG Y E VF+ LDFV++EA ++ I +I+SLVNN+ DYGGR +YV W  
Sbjct: 89  FNEGDYIPLQISPGSYSEDVFKGLDFVVYEAGRFNIKLIISLVNNFEDYGGRKKYVEW-- 148

Query: 149 AAGAQIHNDDDFYTNQLIKGFYTGHVEKVLTRKNTMSGLRYMDDPTIMGWELMNEPRCEV 208
              A +   D+FYTN  +K FY  HV+ VLTRKNT++G  Y DDPTI  WEL+NEPRC  
Sbjct: 149 ---AGLDEPDEFYTNSAVKQFYKNHVKTVLTRKNTITGRMYKDDPTIFSWELINEPRCND 208

Query: 209 DSSGATVNRWVEEMGTYVKSIDKKHLVGIGMEGFYGDSNPSRIKFNPNG-YKVGTDFVTN 268
            ++   +  WV+EM +YVKSID  HL+ IG+EGFYG+S P R  +NP G    GTDF+TN
Sbjct: 209 STASNILQDWVKEMASYVKSIDSNHLLEIGLEGFYGESIPERTVYNPGGRVLTGTDFITN 268

Query: 269 NLIKVIDFATIHAYPDSWLPGQSEAT--KMAFLEEWMKIHWSDSKTILKKPLIFAEFGKS 328
           N I  IDFATIH YPDSWLP QS  T  +  F++ W+  H  D   I+KKPL+  EFGKS
Sbjct: 269 NQIPDIDFATIHIYPDSWLPLQSSRTGEQDTFVDRWIGAHIEDCDNIIKKPLLITEFGKS 328

Query: 329 TKNQNQNQAFSVRDRDSFLTKVYSNIYNLARNGTTMAGGLVWQVMAEGMESYYDGYEIVL 388
           +K       FS+  R+ F  +VY  IY+ AR G +  GG+ WQ+         DGYE+ +
Sbjct: 329 SK----YPGFSLEKRNKFFQRVYDVIYDSARAGGSCTGGVFWQLTTNRTGLLGDGYEVFM 388

Query: 389 SQNPLTTA-IIMQQSKNMAALQH 406
              P TTA +I  QS  +  L++
Sbjct: 389 QAGPNTTAQLIADQSSKLKNLKY 402

BLAST of Moc11g29270 vs. TAIR 10
Match: AT3G10900.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 358.2 bits (918), Expect = 8.6e-99
Identity = 179/406 (44.09%), Postives = 247/406 (60.84%), Query Frame = 0

Query: 1   MRWLVWGVLLVLLGGHSSSSSPAPAPAAFVGIGDGHFQLNGSPFVFNGFNSYWMMSVGAD 60
           M+ L + VLL ++   S     A     FV      F LNG PF  NGFN+YW+     D
Sbjct: 1   MKCLCFIVLLAIVIAQSYVGVEAAPSDGFVSRNGVQFILNGKPFYANGFNAYWLAYEATD 60

Query: 61  PNQRRKVSDAFRDAAAAGLTVCRTWAFADGG-YHALQISPGVYDESVFQALDFVIWEARK 120
           P  R K+++ F++A + GLT+ RTW F DG  Y ALQ +PG YDE  FQ LDFVI EA++
Sbjct: 61  PTTRFKITNVFQNATSLGLTIARTWGFRDGAIYRALQTAPGSYDEQTFQGLDFVIAEAKR 120

Query: 121 YGIWIILSLVNNYGDYGGRPQYVRWARAAGAQIHNDDDFYTNQLIKGFYTGHVEKVLTRK 180
            GI +I+ LVNN+ DYGG+ QYV WAR+ G  + ++DDFY N +IK FY  HV+ VL R 
Sbjct: 121 IGIKLIILLVNNWDDYGGKKQYVDWARSKGEVVSSNDDFYRNPVIKDFYKNHVKTVLNRV 180

Query: 181 NTMSGLRYMDDPTIMGWELMNEPRCEVDSSGATVNRWVEEMGTYVKSIDKKHLVGIGMEG 240
           NT + + Y D+P IM W+LMNEPRC VD SG T+  W+ EM  +VKS+D  HL+  G EG
Sbjct: 181 NTFTKVAYKDEPAIMAWQLMNEPRCGVDKSGKTLMDWINEMAPFVKSVDPNHLLSTGHEG 240

Query: 241 FYGDSNPSR-IKFNP-NGYKVGTDFVTNNLIKVIDFATIHAYPDSWLPGQSEATKMAFLE 300
           FYGDS+P R    NP +   VG DF+ N+ I  IDFA++H   D W     + +++AF++
Sbjct: 241 FYGDSSPERKNSLNPVSANTVGADFIANHNIDAIDFASMHCGSDLWFQRLDQNSRLAFIK 300

Query: 301 EWMKIHWSDSKTILKKPLIFAEFGKSTKNQNQNQAFSVRDRDSFLTKVYSNIYNLARNGT 360
            W++ H  D++ ILKKP+I AEFG      +    +++ +RD   T  Y  IY  A+ G 
Sbjct: 301 RWLEGHIEDAQNILKKPVILAEFGLG----SDTPRYTLANRDGVFTTTYDIIYASAQKGG 360

Query: 361 TMAGGLVWQVMAEGMESYYDGYEIVLSQNPLTTAIIMQQSKNMAAL 404
           + AG L W+V++EGM ++     I+LS    T  II + ++ M  L
Sbjct: 361 SAAGALFWEVISEGMSNFAGPSSIILSDKSSTVNIISEHARKMGLL 402

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022141751.11.9e-241100.00mannan endo-1,4-beta-mannosidase 5-like [Momordica charantia][more]
XP_038890634.12.2e-18980.15mannan endo-1,4-beta-mannosidase 1-like [Benincasa hispida][more]
XP_008459570.13.5e-18777.97PREDICTED: mannan endo-1,4-beta-mannosidase 1-like [Cucumis melo] >TYK00481.1 ma... [more]
XP_004141536.21.0e-18677.92mannan endo-1,4-beta-mannosidase 1 [Cucumis sativus] >KGN52650.1 hypothetical pr... [more]
XP_004141631.34.9e-18175.93mannan endo-1,4-beta-mannosidase 1 [Cucumis sativus] >XP_031740837.1 mannan endo... [more]
Match NameE-valueIdentityDescription
Q9FZ031.4e-14161.80Mannan endo-1,4-beta-mannosidase 2 OS=Solanum lycopersicum OX=4081 GN=MAN2 PE=2 ... [more]
Q6YM501.4e-14161.80Mannan endo-1,4-beta-mannosidase 5 OS=Solanum lycopersicum OX=4081 GN=MAN5 PE=2 ... [more]
O485409.1e-13860.16Mannan endo-1,4-beta-mannosidase 1 OS=Solanum lycopersicum OX=4081 GN=MAN1 PE=1 ... [more]
Q9FUQ61.8e-13356.79Mannan endo-1,4-beta-mannosidase 3 OS=Solanum lycopersicum OX=4081 GN=MAN3 PE=3 ... [more]
Q0JKM93.9e-12053.32Mannan endo-1,4-beta-mannosidase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=M... [more]
Match NameE-valueIdentityDescription
A0A6J1CK639.2e-242100.00Mannan endo-1,4-beta-mannosidase OS=Momordica charantia OX=3673 GN=LOC111012035 ... [more]
A0A5D3BKZ31.7e-18777.97Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3CB041.7e-18777.97Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103498662 PE=3 SV... [more]
A0A0A0KSS25.0e-18777.92Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_5G648710 PE=3... [more]
A0A5A7TCI28.2e-16671.53Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
Match NameE-valueIdentityDescription
AT5G66460.12.2e-11550.80Glycosyl hydrolase superfamily protein [more]
AT3G10890.15.9e-10846.08Glycosyl hydrolase superfamily protein [more]
AT5G01930.11.3e-10750.68Glycosyl hydrolase superfamily protein [more]
AT1G02310.11.2e-10048.56Glycosyl hydrolase superfamily protein [more]
AT3G10900.18.6e-9944.09Glycosyl hydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001547Glycoside hydrolase, family 5PFAMPF00150Cellulasecoord: 40..330
e-value: 2.4E-17
score: 63.3
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 27..404
e-value: 1.3E-126
score: 424.5
NoneNo IPR availablePANTHERPTHR31451:SF53MANNAN ENDO-1,4-BETA-MANNOSIDASE-LIKE PROTEINcoord: 2..405
IPR045053Mannan endo-1,4-beta-mannosidase-likePANTHERPTHR31451FAMILY NOT NAMEDcoord: 2..405
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 29..390

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc11g29270.1Moc11g29270.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071704 organic substance metabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0016985 mannan endo-1,4-beta-mannosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds