Moc11g27710 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc11g27710
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptionalpha/beta-Hydrolases superfamily protein
Locationchr11: 20271318 .. 20272334 (-)
RNA-Seq ExpressionMoc11g27710
SyntenyMoc11g27710
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCATCGTCAAGTTGTTCTTCCGAAGTTTCAAACCGGCCCCAGCTTCCATGGAAGTATCGGGTGATGCTCCGATTTGCTCCTCTGTTCTTCCACATTGCTTTCCGATCGGATTCCACCGTCAATCGCCGGCTTCTCCAATTTGTTGACCGGAAAGCCCCCGCCTCCTCCGAGCCACTCGACGGCATTTCCTCTTCCGATCTCACCATCGATACTTCTCGAAGCCTGTGGATCCGAATCTACACCCCCACTGCTCACGGCGGCGATTCTCCCAAGCTGCCGGTTGTTCTGTTCTTCCACGGCGGAGGATTCGCCCTCTTTCACGCCGATACACTTGCCTGCGACGTTGCCGCTCGCCGGTACGCCGGAGAGTTACGCGCCGTCGTAATCTCCGTCAACTACCGCCTCGCTCCGGAGTTCCGATTTCCGTCTCCGTACGACGACGGATTCGACGCTTTGAAATTTCTGGACGAAATCGACGACGGCGTGCTGCCGGCGGCGGCCGATCTGGGCCGTTGCTTCATCCTCGGCGAGAGCGCCGGCGGGAATCTGGGGCACCACGTGGCGGTGAGGGCGAGCGAGTACGCGTTCAAGAAGGTGACGGTGGCCGGATTTGTCGCCGTGCAGCCGTTTTTCGGCGGAGAGGAGAGAACAAAGTCGGAGATCCGGCTGTGTAATCAGCCTCCATTCACTCGGGAAATGGCGGATTGGTTTTGGAAGGCGTTTTTGCCGGTCGGGTCGGACCGGGACCACGCGGCGGCGAACGTGTTCGGACCCAAGGGAGAGGACATTTCCAGGAGGAAGTTTCCGGCGACGCTGATTTTGGTCGGAGGATTTGATCTGTTATGTGATCGACAGAGGATGTATGAGGAGTGGCTGAAGAGATCGGGAAAGAAAGTAGAGCTTGTGGAGTTTTCAAATGCAATTCATGGATTCTCCGGCCTCCCGGACCTGCCGGAATATTCTTCCATGATTAGAGAGATGAAGAATTTCATGGAAAGGGCTCCAAAATTTTAG

mRNA sequence

ATGGCATCGTCAAGTTGTTCTTCCGAAGTTTCAAACCGGCCCCAGCTTCCATGGAAGTATCGGGTGATGCTCCGATTTGCTCCTCTGTTCTTCCACATTGCTTTCCGATCGGATTCCACCGTCAATCGCCGGCTTCTCCAATTTGTTGACCGGAAAGCCCCCGCCTCCTCCGAGCCACTCGACGGCATTTCCTCTTCCGATCTCACCATCGATACTTCTCGAAGCCTGTGGATCCGAATCTACACCCCCACTGCTCACGGCGGCGATTCTCCCAAGCTGCCGGTTGTTCTGTTCTTCCACGGCGGAGGATTCGCCCTCTTTCACGCCGATACACTTGCCTGCGACGTTGCCGCTCGCCGGTACGCCGGAGAGTTACGCGCCGTCGTAATCTCCGTCAACTACCGCCTCGCTCCGGAGTTCCGATTTCCGTCTCCGTACGACGACGGATTCGACGCTTTGAAATTTCTGGACGAAATCGACGACGGCGTGCTGCCGGCGGCGGCCGATCTGGGCCGTTGCTTCATCCTCGGCGAGAGCGCCGGCGGGAATCTGGGGCACCACGTGGCGGTGAGGGCGAGCGAGTACGCGTTCAAGAAGGTGACGGTGGCCGGATTTGTCGCCGTGCAGCCGTTTTTCGGCGGAGAGGAGAGAACAAAGTCGGAGATCCGGCTGTGTAATCAGCCTCCATTCACTCGGGAAATGGCGGATTGGTTTTGGAAGGCGTTTTTGCCGGTCGGGTCGGACCGGGACCACGCGGCGGCGAACGTGTTCGGACCCAAGGGAGAGGACATTTCCAGGAGGAAGTTTCCGGCGACGCTGATTTTGGTCGGAGGATTTGATCTGTTATGTGATCGACAGAGGATGTATGAGGAGTGGCTGAAGAGATCGGGAAAGAAAGTAGAGCTTGTGGAGTTTTCAAATGCAATTCATGGATTCTCCGGCCTCCCGGACCTGCCGGAATATTCTTCCATGATTAGAGAGATGAAGAATTTCATGGAAAGGGCTCCAAAATTTTAG

Coding sequence (CDS)

ATGGCATCGTCAAGTTGTTCTTCCGAAGTTTCAAACCGGCCCCAGCTTCCATGGAAGTATCGGGTGATGCTCCGATTTGCTCCTCTGTTCTTCCACATTGCTTTCCGATCGGATTCCACCGTCAATCGCCGGCTTCTCCAATTTGTTGACCGGAAAGCCCCCGCCTCCTCCGAGCCACTCGACGGCATTTCCTCTTCCGATCTCACCATCGATACTTCTCGAAGCCTGTGGATCCGAATCTACACCCCCACTGCTCACGGCGGCGATTCTCCCAAGCTGCCGGTTGTTCTGTTCTTCCACGGCGGAGGATTCGCCCTCTTTCACGCCGATACACTTGCCTGCGACGTTGCCGCTCGCCGGTACGCCGGAGAGTTACGCGCCGTCGTAATCTCCGTCAACTACCGCCTCGCTCCGGAGTTCCGATTTCCGTCTCCGTACGACGACGGATTCGACGCTTTGAAATTTCTGGACGAAATCGACGACGGCGTGCTGCCGGCGGCGGCCGATCTGGGCCGTTGCTTCATCCTCGGCGAGAGCGCCGGCGGGAATCTGGGGCACCACGTGGCGGTGAGGGCGAGCGAGTACGCGTTCAAGAAGGTGACGGTGGCCGGATTTGTCGCCGTGCAGCCGTTTTTCGGCGGAGAGGAGAGAACAAAGTCGGAGATCCGGCTGTGTAATCAGCCTCCATTCACTCGGGAAATGGCGGATTGGTTTTGGAAGGCGTTTTTGCCGGTCGGGTCGGACCGGGACCACGCGGCGGCGAACGTGTTCGGACCCAAGGGAGAGGACATTTCCAGGAGGAAGTTTCCGGCGACGCTGATTTTGGTCGGAGGATTTGATCTGTTATGTGATCGACAGAGGATGTATGAGGAGTGGCTGAAGAGATCGGGAAAGAAAGTAGAGCTTGTGGAGTTTTCAAATGCAATTCATGGATTCTCCGGCCTCCCGGACCTGCCGGAATATTCTTCCATGATTAGAGAGATGAAGAATTTCATGGAAAGGGCTCCAAAATTTTAG

Protein sequence

MASSSCSSEVSNRPQLPWKYRVMLRFAPLFFHIAFRSDSTVNRRLLQFVDRKAPASSEPLDGISSSDLTIDTSRSLWIRIYTPTAHGGDSPKLPVVLFFHGGGFALFHADTLACDVAARRYAGELRAVVISVNYRLAPEFRFPSPYDDGFDALKFLDEIDDGVLPAAADLGRCFILGESAGGNLGHHVAVRASEYAFKKVTVAGFVAVQPFFGGEERTKSEIRLCNQPPFTREMADWFWKAFLPVGSDRDHAAANVFGPKGEDISRRKFPATLILVGGFDLLCDRQRMYEEWLKRSGKKVELVEFSNAIHGFSGLPDLPEYSSMIREMKNFMERAPKF
Homology
BLAST of Moc11g27710 vs. NCBI nr
Match: XP_022159210.1 (probable carboxylesterase 18 [Momordica charantia])

HSP 1 Score: 695.7 bits (1794), Expect = 2.0e-196
Identity = 337/338 (99.70%), Postives = 337/338 (99.70%), Query Frame = 0

Query: 1   MASSSCSSEVSNRPQLPWKYRVMLRFAPLFFHIAFRSDSTVNRRLLQFVDRKAPASSEPL 60
           MASSSCSSEVSNRPQLPWKYRVMLRFAPLFFHIAFRSDSTVNRRLLQFVDRKAPASSEPL
Sbjct: 1   MASSSCSSEVSNRPQLPWKYRVMLRFAPLFFHIAFRSDSTVNRRLLQFVDRKAPASSEPL 60

Query: 61  DGISSSDLTIDTSRSLWIRIYTPTAHGGDSPKLPVVLFFHGGGFALFHADTLACDVAARR 120
           DGISSSDLTIDTSRSLWIRIYTPTAHGGDSPKLPVVLFFHGGGFALFHADTLACDVAARR
Sbjct: 61  DGISSSDLTIDTSRSLWIRIYTPTAHGGDSPKLPVVLFFHGGGFALFHADTLACDVAARR 120

Query: 121 YAGELRAVVISVNYRLAPEFRFPSPYDDGFDALKFLDEIDDGVLPAAADLGRCFILGESA 180
           YAGELRAVVISVNYRLAPEFRFPSPYDDGFDALKFLDEIDDGVLPAAADLGRCFILGESA
Sbjct: 121 YAGELRAVVISVNYRLAPEFRFPSPYDDGFDALKFLDEIDDGVLPAAADLGRCFILGESA 180

Query: 181 GGNLGHHVAVRASEYAFKKVTVAGFVAVQPFFGGEERTKSEIRLCNQPPFTREMADWFWK 240
           GGNLGHHVAVRASEYA KKVTVAGFVAVQPFFGGEERTKSEIRLCNQPPFTREMADWFWK
Sbjct: 181 GGNLGHHVAVRASEYASKKVTVAGFVAVQPFFGGEERTKSEIRLCNQPPFTREMADWFWK 240

Query: 241 AFLPVGSDRDHAAANVFGPKGEDISRRKFPATLILVGGFDLLCDRQRMYEEWLKRSGKKV 300
           AFLPVGSDRDHAAANVFGPKGEDISRRKFPATLILVGGFDLLCDRQRMYEEWLKRSGKKV
Sbjct: 241 AFLPVGSDRDHAAANVFGPKGEDISRRKFPATLILVGGFDLLCDRQRMYEEWLKRSGKKV 300

Query: 301 ELVEFSNAIHGFSGLPDLPEYSSMIREMKNFMERAPKF 339
           ELVEFSNAIHGFSGLPDLPEYSSMIREMKNFMERAPKF
Sbjct: 301 ELVEFSNAIHGFSGLPDLPEYSSMIREMKNFMERAPKF 338

BLAST of Moc11g27710 vs. NCBI nr
Match: XP_022925491.1 (probable carboxylesterase 18 [Cucurbita moschata])

HSP 1 Score: 446.0 bits (1146), Expect = 2.8e-121
Identity = 221/334 (66.17%), Postives = 258/334 (77.25%), Query Frame = 0

Query: 1   MASSSCSSEVSNRPQLPWKYRVMLRFAPLFFHIAFRSDSTVNRRLLQFVDRKAPASSEPL 60
           M  SS SSE+S   QLPWK+R+ LRFA L F+ + RSD TVNRRLL F+D K P +   +
Sbjct: 1   MQPSSVSSEIST-SQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSI 60

Query: 61  DGISSSDLTIDTSRSLWIRIYTPTAHGGDSPKLPVVLFFHGGGFALFHADTLACDVAARR 120
             +SSSDLTID SR L++RI+TPT    DS   PV+ +FHGGGFA   AD  + D+AARR
Sbjct: 61  HAVSSSDLTIDDSRDLFLRIFTPTVAATDSVLPPVIFYFHGGGFAFGSADATSMDMAARR 120

Query: 121 YAGELRAVVISVNYRLAPEFRFPSPYDDGFDALKFLDEI-DDGVLPAAADLGRCFILGES 180
           ++ EL AVVISVNYRLAP+FRFP  YDDGFDA+KF+DE+ DD  L   ADL RCFILGES
Sbjct: 121 FSKELGAVVISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSRCFILGES 180

Query: 181 AGGNLGHHVAVRASEYAFKKVTVAGFVAVQPFFGGEERTKSEIRLCNQPPFTREMADWFW 240
           AGGNLGHHVAVRASEYAFK+V V GF+A QPFFGGEERT+SEIRLCNQPP T ++ADWFW
Sbjct: 181 AGGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFW 240

Query: 241 KAFLPVGSDRDHAAANVFGPKGEDIS-RRKFPATLILVGGFDLLCDRQRMYEEWLKRSGK 300
           KAFLP G DRDHAAANV GP G D+S  R FPAT+IL GG DLL DRQR Y E LKR GK
Sbjct: 241 KAFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDLLIDRQRRYYEELKRMGK 300

Query: 301 KVELVEFSNAIHGFSGLPDLPEYSSMIREMKNFM 333
           +V+LV F+N +HGF    D PEYS MI+EM++F+
Sbjct: 301 EVKLVVFTNGVHGFFSFEDPPEYSLMIKEMRDFI 333

BLAST of Moc11g27710 vs. NCBI nr
Match: XP_022973390.1 (probable carboxylesterase 18 [Cucurbita maxima])

HSP 1 Score: 444.5 bits (1142), Expect = 8.1e-121
Identity = 213/319 (66.77%), Postives = 254/319 (79.62%), Query Frame = 0

Query: 15  QLPWKYRVMLRFAPLFFHIAFRSDSTVNRRLLQFVDRKAPASSEPLDGISSSDLTIDTSR 74
           +LPWK+R+MLR   +  +   RSD T+NR L   +DR+ P SS+P+DG+SSSDL ID SR
Sbjct: 13  RLPWKHRLMLRLGTIATNTFCRSDFTINRWLTGILDRRTPPSSKPIDGVSSSDLLIDKSR 72

Query: 75  SLWIRIYTPTAHGGDSPKLPVVLFFHGGGFALFHADTLACDVAARRYAGELRAVVISVNY 134
           +LW+RI+TP     D   LPV+ ++HGGGFA  +AD+      A ++A +LRAVVISVNY
Sbjct: 73  NLWVRIFTPI---DDVQSLPVIFYYHGGGFAFSYADSSLYHTTAHQFAKQLRAVVISVNY 132

Query: 135 RLAPEFRFPSPYDDGFDALKFLDEIDDGVLPAAADLGRCFILGESAGGNLGHHVAVRASE 194
           RLAPEFRFP  YDDGFDALKF+DEIDDG LPA+ADLGR FILGESAGGNLGHHVAVRASE
Sbjct: 133 RLAPEFRFPCQYDDGFDALKFIDEIDDGTLPASADLGRSFILGESAGGNLGHHVAVRASE 192

Query: 195 YAFKKVTVAGFVAVQPFFGGEERTKSEIRLCNQPPFTREMADWFWKAFLPVGSDRDHAAA 254
           YAFKK+ + GF+A QPF G EERT+SEIRLCNQPP + ++ DWFWKAFLPVGSDRDHAAA
Sbjct: 193 YAFKKLKLIGFIATQPFIGAEERTESEIRLCNQPPLSLKLTDWFWKAFLPVGSDRDHAAA 252

Query: 255 NVFGPKGEDISR-RKFPATLILVGGFDLLCDRQRMYEEWLKRSGKKVELVEFSNAIHGFS 314
           NVFGP G DIS   KFPAT++LVGGFDLL D QR Y E LKR GK+V++VE  NAIHGF 
Sbjct: 253 NVFGPNGRDISGVEKFPATVVLVGGFDLLQDGQRRYYEGLKRMGKEVKMVELPNAIHGFF 312

Query: 315 GLPDLPEYSSMIREMKNFM 333
             PDLPEYSSMI+ +++F+
Sbjct: 313 CFPDLPEYSSMIQAVRDFI 328

BLAST of Moc11g27710 vs. NCBI nr
Match: KAG6607539.1 (putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 444.5 bits (1142), Expect = 8.1e-121
Identity = 215/319 (67.40%), Postives = 253/319 (79.31%), Query Frame = 0

Query: 15  QLPWKYRVMLRFAPLFFHIAFRSDSTVNRRLLQFVDRKAPASSEPLDGISSSDLTIDTSR 74
           +LPWK+R+MLR   +  +   RSD T+NR L   +DR+ P SS P+DGISSSDL ID SR
Sbjct: 13  RLPWKHRLMLRLGTIATNTFCRSDFTINRWLTGILDRRTPPSSIPIDGISSSDLLIDPSR 72

Query: 75  SLWIRIYTPTAHGGDSPKLPVVLFFHGGGFALFHADTLACDVAARRYAGELRAVVISVNY 134
           +LW+RI+TP     D   LPV+ ++HGGGFA  +AD+      A ++A +LRAVVISVNY
Sbjct: 73  NLWVRIFTPI---DDVQSLPVIFYYHGGGFAFSYADSSLYHTTAHQFAKQLRAVVISVNY 132

Query: 135 RLAPEFRFPSPYDDGFDALKFLDEIDDGVLPAAADLGRCFILGESAGGNLGHHVAVRASE 194
           RLAPEFRFP  YDDGFDALKF+DEIDDG LPA+ADLGR FILGESAGGNLGHHVAVRASE
Sbjct: 133 RLAPEFRFPCQYDDGFDALKFIDEIDDGTLPASADLGRSFILGESAGGNLGHHVAVRASE 192

Query: 195 YAFKKVTVAGFVAVQPFFGGEERTKSEIRLCNQPPFTREMADWFWKAFLPVGSDRDHAAA 254
           YAFKK+ + GF+A QPFFG EERT+SEIRLCNQ P    ++DWFWKAFLPVGSDRDHAAA
Sbjct: 193 YAFKKLKLIGFIATQPFFGAEERTESEIRLCNQAPLRLRLSDWFWKAFLPVGSDRDHAAA 252

Query: 255 NVFGPKGEDISR-RKFPATLILVGGFDLLCDRQRMYEEWLKRSGKKVELVEFSNAIHGFS 314
           NVFGP G DIS   +FPAT++LVGGFDLL D QR Y E LKR GK+V++VE  NAIHGF 
Sbjct: 253 NVFGPNGRDISGVERFPATVVLVGGFDLLQDGQRRYYEGLKRMGKEVKMVELPNAIHGFF 312

Query: 315 GLPDLPEYSSMIREMKNFM 333
             PDLPEYSSMI+EM++F+
Sbjct: 313 CFPDLPEYSSMIQEMRDFI 328

BLAST of Moc11g27710 vs. NCBI nr
Match: KAG6592331.1 (putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. sororia] >KAG7025153.1 putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 442.6 bits (1137), Expect = 3.1e-120
Identity = 219/334 (65.57%), Postives = 257/334 (76.95%), Query Frame = 0

Query: 1   MASSSCSSEVSNRPQLPWKYRVMLRFAPLFFHIAFRSDSTVNRRLLQFVDRKAPASSEPL 60
           M   S SSE+S   QLPWK+R+ LRFA L F+ + RSD TVNRRLL F+D K P +   +
Sbjct: 1   MQPFSVSSEIST-SQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSI 60

Query: 61  DGISSSDLTIDTSRSLWIRIYTPTAHGGDSPKLPVVLFFHGGGFALFHADTLACDVAARR 120
             +SSSDLTID SR L++RI+TPT    DS   PV+ +FHGGGFA   A   + D+AARR
Sbjct: 61  HAVSSSDLTIDDSRDLFLRIFTPTVAATDSVLPPVIFYFHGGGFAFGSAAATSMDMAARR 120

Query: 121 YAGELRAVVISVNYRLAPEFRFPSPYDDGFDALKFLDEI-DDGVLPAAADLGRCFILGES 180
           ++ EL AVVISVNYRLAP+FRFP  YDDGFDA+KF+DE+ DD  L   ADL RCFILGES
Sbjct: 121 FSKELGAVVISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSRCFILGES 180

Query: 181 AGGNLGHHVAVRASEYAFKKVTVAGFVAVQPFFGGEERTKSEIRLCNQPPFTREMADWFW 240
           AGGNLGHHVAVRASEYAFK+V V GF+A QPFFGGEERT+SEIRLCNQPP T ++ADWFW
Sbjct: 181 AGGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFW 240

Query: 241 KAFLPVGSDRDHAAANVFGPKGEDIS-RRKFPATLILVGGFDLLCDRQRMYEEWLKRSGK 300
           KAFLP G DRDHAAANV GP G D+S  R FPAT+IL GG D+L DRQR Y E LKR GK
Sbjct: 241 KAFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDILIDRQRRYYEELKRMGK 300

Query: 301 KVELVEFSNAIHGFSGLPDLPEYSSMIREMKNFM 333
           +V+LV F+N +HGF    DLPEYS MI+EM++F+
Sbjct: 301 EVKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFI 333

BLAST of Moc11g27710 vs. ExPASy Swiss-Prot
Match: Q9LT10 (Probable carboxylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=CXE18 PE=1 SV=1)

HSP 1 Score: 311.2 bits (796), Expect = 1.4e-83
Identity = 154/329 (46.81%), Postives = 208/329 (63.22%), Query Frame = 0

Query: 8   SEVSNRPQLPWKYRVMLRFAPLFFHIAFRSDSTVNRRLLQFVDRKAPASSEPLDGISSSD 67
           S+ + +  LP K R+ L         A R D T+NRR L+  D +AP + +P++ +S+SD
Sbjct: 5   SQPNQKLTLPLKTRIALTVISTMTDNAQRPDGTINRRFLRLFDFRAPPNPKPVNIVSTSD 64

Query: 68  LTIDTSRSLWIRIYTPTAHGGDSPKLPVVLFFHGGGFALFHADTLACDVAARRYAGELRA 127
             +D SR LW R+YTP   G    K+PVV+FFHGGGFA    +    D   RR+A +L A
Sbjct: 65  FVVDQSRDLWFRLYTPHVSG---DKIPVVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPA 124

Query: 128 VVISVNYRLAPEFRFPSPYDDGFDALKFLDEIDDGVLPAAADLGRCFILGESAGGNLGHH 187
            VISVNYRLAPE R+P+ YDDGFDALK+++E    +LPA ADL RCF  G+SAGGN+ H+
Sbjct: 125 YVISVNYRLAPEHRYPAQYDDGFDALKYIEENHGSILPANADLSRCFFAGDSAGGNIAHN 184

Query: 188 VAV---RASEYAFKKVTVAGFVAVQPFFGGEERTKSEIRLCNQPPFTREMADWFWKAFLP 247
           VA+   R    +F  V + G +++QPFFGGEERT++E +L   P  + +  DW WKA   
Sbjct: 185 VAIRICREPRSSFTAVKLIGLISIQPFFGGEERTEAEKQLVGAPLVSPDRTDWCWKA--- 244

Query: 248 VGSDRDHAAANVFGPKGEDISRRKFPATLILVGGFDLLCDRQRMYEEWLKRSGKKVELVE 307
           +G +RDH A NV GP   DIS   +P T+++V GFD L D QR Y EWLK  GKK  L+E
Sbjct: 245 MGLNRDHEAVNVGGPNAVDISGLDYPETMVVVAGFDPLKDWQRSYYEWLKLCGKKATLIE 304

Query: 308 FSNAIHGFSGLPDLPEYSSMIREMKNFME 334
           + N  H F   P+LPE   +I  +K+F++
Sbjct: 305 YPNMFHAFYIFPELPEAGQLIMRIKDFVD 327

BLAST of Moc11g27710 vs. ExPASy Swiss-Prot
Match: Q940G6 (Gibberellin receptor GID1C OS=Arabidopsis thaliana OX=3702 GN=GID1C PE=1 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 5.2e-62
Identity = 131/344 (38.08%), Postives = 198/344 (57.56%), Query Frame = 0

Query: 1   MASSSCSSEVSNRPQLPWKYRVMLRFAPLFFHIAFRSDSTVNRRLLQFVDRKAPASSEPL 60
           MA S   + + ++  +P    V++    L +++  R D T NR L +F+DRK PA++ P+
Sbjct: 1   MAGSEEVNLIESKTVVPLNTWVLISNFKLAYNLLRRPDGTFNRHLAEFLDRKVPANANPV 60

Query: 61  DGISSSDLTIDTSRSLWIRIYTPTAHGGDSPKL------------PVVLFFHGGGFALFH 120
           +G+ S D+ ID   +L  R+Y P A  G SP +            PV++FFHGG FA   
Sbjct: 61  NGVFSFDVIIDRQTNLLSRVYRP-ADAGTSPSITDLQNPVDGEIVPVIVFFHGGSFAHSS 120

Query: 121 ADTLACDVAARRYAGELRAVVISVNYRLAPEFRFPSPYDDGFDALKFLDEIDDGVLPAAA 180
           A++   D   RR  G   AVV+SVNYR APE R+P  YDDG+  LK+++     +     
Sbjct: 121 ANSAIYDTLCRRLVGLCGAVVVSVNYRRAPENRYPCAYDDGWAVLKWVNS-SSWLRSKKD 180

Query: 181 DLGRCFILGESAGGNLGHHVAVRASEYAFKKVTVAGFVAVQPFFGGEERTKSEIRLCNQP 240
              R F+ G+S+GGN+ H+VAVRA E    ++ V G + + P FGG ERT+SE RL  + 
Sbjct: 181 SKVRIFLAGDSSGGNIVHNVAVRAVE---SRIDVLGNILLNPMFGGTERTESEKRLDGKY 240

Query: 241 PFTREMADWFWKAFLPVGSDRDHAAANVFGPKGEDISRRKFPATLILVGGFDLLCDRQRM 300
             T    DW+W+AFLP G DR+H A + FGP+ + +    FP +L++V G DL+ D Q  
Sbjct: 241 FVTVRDRDWYWRAFLPEGEDREHPACSPFGPRSKSLEGLSFPKSLVVVAGLDLIQDWQLK 300

Query: 301 YEEWLKRSGKKVELVEFSNAIHGFSGLPDLPEYSSMIREMKNFM 333
           Y E LK++G++V+L+    A  GF  LP+   + +++ E+  F+
Sbjct: 301 YAEGLKKAGQEVKLLYLEQATIGFYLLPNNNHFHTVMDEIAAFV 339

BLAST of Moc11g27710 vs. ExPASy Swiss-Prot
Match: Q6L545 (Gibberellin receptor GID1 OS=Oryza sativa subsp. japonica OX=39947 GN=GID1 PE=1 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 5.2e-62
Identity = 135/353 (38.24%), Postives = 195/353 (55.24%), Query Frame = 0

Query: 1   MASSSCSSEVSNRPQLPWKYRVMLRFAPLFFHIAFRSDSTVNRRLLQFVDRKAPASSEPL 60
           MA S   +    +  +P    V++    L ++I  R+D T  R L +++DR+ PA++ PL
Sbjct: 1   MAGSDEVNRNECKTVVPLHTWVLISNFKLSYNILRRADGTFERDLGEYLDRRVPANARPL 60

Query: 61  DGISSSDLTIDTSRSLWIRIYTPTAHGG-----------------DSP---KLPVVLFFH 120
           +G+SS D  ID S  L +RIY   A G                  D+P     PV++FFH
Sbjct: 61  EGVSSFDHIIDQSVGLEVRIYRAAAEGDAEEGAAAVTRPILEFLTDAPAAEPFPVIIFFH 120

Query: 121 GGGFALFHADTLACDVAARRYAGELRAVVISVNYRLAPEFRFPSPYDDGFDALKFLDEID 180
           GG F    A +   D   RR+    + VV+SVNYR APE R+P  YDDG+ ALK++  + 
Sbjct: 121 GGSFVHSSASSTIYDSLCRRFVKLSKGVVVSVNYRRAPEHRYPCAYDDGWTALKWV--MS 180

Query: 181 DGVLPAAADL-GRCFILGESAGGNLGHHVAVRASEYAFKKVTVAGFVAVQPFFGGEERTK 240
              + +  D   R F+ G+S+GGN+ HHVAVRA++   + V V G + +   FGG ERT+
Sbjct: 181 QPFMRSGGDAQARVFLSGDSSGGNIAHHVAVRAAD---EGVKVCGNILLNAMFGGTERTE 240

Query: 241 SEIRLCNQPPFTREMADWFWKAFLPVGSDRDHAAANVFGPKGEDISRRKFPATLILVGGF 300
           SE RL  +   T +  DW+WKA+LP  +DRDH A N FGP G  +    F  +LI+V G 
Sbjct: 241 SERRLDGKYFVTLQDRDWYWKAYLPEDADRDHPACNPFGPNGRRLGGLPFAKSLIIVSGL 300

Query: 301 DLLCDRQRMYEEWLKRSGKKVELVEFSNAIHGFSGLPDLPEYSSMIREMKNFM 333
           DL CDRQ  Y + L+  G  V++V+  NA  GF  LP+   Y  ++ E+ +F+
Sbjct: 301 DLTCDRQLAYADALREDGHHVKVVQCENATVGFYLLPNTVHYHEVMEEISDFL 348

BLAST of Moc11g27710 vs. ExPASy Swiss-Prot
Match: Q9MAA7 (Gibberellin receptor GID1A OS=Arabidopsis thaliana OX=3702 GN=GID1A PE=1 SV=1)

HSP 1 Score: 230.7 bits (587), Expect = 2.4e-59
Identity = 128/346 (36.99%), Postives = 194/346 (56.07%), Query Frame = 0

Query: 1   MASSSCSSEVSNRPQLPWKYRVMLRFAPLFFHIAFRSDSTVNRRLLQFVDRKAPASSEPL 60
           MA+S   + + +R  +P    V++    + ++I  R D T NR L +++DRK  A++ P+
Sbjct: 1   MAASDEVNLIESRTVVPLNTWVLISNFKVAYNILRRPDGTFNRHLAEYLDRKVTANANPV 60

Query: 61  DGISSSDLTIDTSRSLWIRIYTPTAHGGDSPK-------------LPVVLFFHGGGFALF 120
           DG+ S D+ ID   +L  R+Y P     + P              +PV+LFFHGG FA  
Sbjct: 61  DGVFSFDVLIDRRINLLSRVYRPAYADQEQPPSILDLEKPVDGDIVPVILFFHGGSFAHS 120

Query: 121 HADTLACDVAARRYAGELRAVVISVNYRLAPEFRFPSPYDDGFDALKFLDEIDDGVLPAA 180
            A++   D   RR  G  + VV+SVNYR APE  +P  YDDG+ AL +++      L + 
Sbjct: 121 SANSAIYDTLCRRLVGLCKCVVVSVNYRRAPENPYPCAYDDGWIALNWVN--SRSWLKSK 180

Query: 181 ADLG-RCFILGESAGGNLGHHVAVRASEYAFKKVTVAGFVAVQPFFGGEERTKSEIRLCN 240
            D     F+ G+S+GGN+ H+VA+RA E     + V G + + P FGG ERT+SE  L  
Sbjct: 181 KDSKVHIFLAGDSSGGNIAHNVALRAGE---SGIDVLGNILLNPMFGGNERTESEKSLDG 240

Query: 241 QPPFTREMADWFWKAFLPVGSDRDHAAANVFGPKGEDISRRKFPATLILVGGFDLLCDRQ 300
           +   T    DW+WKAFLP G DR+H A N F P+G+ +    FP +L++V G DL+ D Q
Sbjct: 241 KYFVTVRDRDWYWKAFLPEGEDREHPACNPFSPRGKSLEGVSFPKSLVVVAGLDLIRDWQ 300

Query: 301 RMYEEWLKRSGKKVELVEFSNAIHGFSGLPDLPEYSSMIREMKNFM 333
             Y E LK++G++V+L+    A  GF  LP+   + +++ E+  F+
Sbjct: 301 LAYAEGLKKAGQEVKLMHLEKATVGFYLLPNNNHFHNVMDEISAFV 341

BLAST of Moc11g27710 vs. ExPASy Swiss-Prot
Match: Q9LYC1 (Gibberellin receptor GID1B OS=Arabidopsis thaliana OX=3702 GN=GID1B PE=1 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 5.6e-56
Identity = 124/332 (37.35%), Postives = 188/332 (56.63%), Query Frame = 0

Query: 16  LPWKYRVMLRFAPLFFHIAFRSDSTVNRRLLQFVDRKAPASSEPLDGISSSDLTIDTSRS 75
           +P    V++    L + +  R D + NR L +F+DRK PA+S PLDG+ S D  +D++ +
Sbjct: 16  VPLNTWVLISNFKLAYKVLRRPDGSFNRDLAEFLDRKVPANSFPLDGVFSFD-HVDSTTN 75

Query: 76  LWIRIYTP------TAHG--------GDSPKLPVVLFFHGGGFALFHADTLACDVAARRY 135
           L  RIY P      T HG          +  +PV++FFHGG F    A++   D   RR 
Sbjct: 76  LLTRIYQPASLLHQTRHGTLELTKPLSTTEIVPVLIFFHGGSFTHSSANSAIYDTFCRRL 135

Query: 136 AGELRAVVISVNYRLAPEFRFPSPYDDGFDALKFLDEIDDGVLPAAADLG-RCFILGESA 195
                 VV+SV+YR +PE R+P  YDDG++AL ++       L +  D     ++ G+S+
Sbjct: 136 VTICGVVVVSVDYRRSPEHRYPCAYDDGWNALNWVK--SRVWLQSGKDSNVYVYLAGDSS 195

Query: 196 GGNLGHHVAVRASEYAFKKVTVAGFVAVQPFFGGEERTKSEIRLCNQPPFTREMADWFWK 255
           GGN+ H+VAVRA+    + V V G + + P FGG+ERT+SE  L  +   T +  DW+W+
Sbjct: 196 GGNIAHNVAVRATN---EGVKVLGNILLHPMFGGQERTQSEKTLDGKYFVTIQDRDWYWR 255

Query: 256 AFLPVGSDRDHAAANVFGPKGEDISRRKFPATLILVGGFDLLCDRQRMYEEWLKRSGKKV 315
           A+LP G DRDH A N FGP+G+ +    FP +L++V G DL+ D Q  Y + LK++G +V
Sbjct: 256 AYLPEGEDRDHPACNPFGPRGQSLKGVNFPKSLVVVAGLDLVQDWQLAYVDGLKKTGLEV 315

Query: 316 ELVEFSNAIHGFSGLPDLPEYSSMIREMKNFM 333
            L+    A  GF  LP+   +  ++ E+  F+
Sbjct: 316 NLLYLKQATIGFYFLPNNDHFHCLMEELNKFV 341

BLAST of Moc11g27710 vs. ExPASy TrEMBL
Match: A0A6J1DY15 (probable carboxylesterase 18 OS=Momordica charantia OX=3673 GN=LOC111025630 PE=4 SV=1)

HSP 1 Score: 695.7 bits (1794), Expect = 9.8e-197
Identity = 337/338 (99.70%), Postives = 337/338 (99.70%), Query Frame = 0

Query: 1   MASSSCSSEVSNRPQLPWKYRVMLRFAPLFFHIAFRSDSTVNRRLLQFVDRKAPASSEPL 60
           MASSSCSSEVSNRPQLPWKYRVMLRFAPLFFHIAFRSDSTVNRRLLQFVDRKAPASSEPL
Sbjct: 1   MASSSCSSEVSNRPQLPWKYRVMLRFAPLFFHIAFRSDSTVNRRLLQFVDRKAPASSEPL 60

Query: 61  DGISSSDLTIDTSRSLWIRIYTPTAHGGDSPKLPVVLFFHGGGFALFHADTLACDVAARR 120
           DGISSSDLTIDTSRSLWIRIYTPTAHGGDSPKLPVVLFFHGGGFALFHADTLACDVAARR
Sbjct: 61  DGISSSDLTIDTSRSLWIRIYTPTAHGGDSPKLPVVLFFHGGGFALFHADTLACDVAARR 120

Query: 121 YAGELRAVVISVNYRLAPEFRFPSPYDDGFDALKFLDEIDDGVLPAAADLGRCFILGESA 180
           YAGELRAVVISVNYRLAPEFRFPSPYDDGFDALKFLDEIDDGVLPAAADLGRCFILGESA
Sbjct: 121 YAGELRAVVISVNYRLAPEFRFPSPYDDGFDALKFLDEIDDGVLPAAADLGRCFILGESA 180

Query: 181 GGNLGHHVAVRASEYAFKKVTVAGFVAVQPFFGGEERTKSEIRLCNQPPFTREMADWFWK 240
           GGNLGHHVAVRASEYA KKVTVAGFVAVQPFFGGEERTKSEIRLCNQPPFTREMADWFWK
Sbjct: 181 GGNLGHHVAVRASEYASKKVTVAGFVAVQPFFGGEERTKSEIRLCNQPPFTREMADWFWK 240

Query: 241 AFLPVGSDRDHAAANVFGPKGEDISRRKFPATLILVGGFDLLCDRQRMYEEWLKRSGKKV 300
           AFLPVGSDRDHAAANVFGPKGEDISRRKFPATLILVGGFDLLCDRQRMYEEWLKRSGKKV
Sbjct: 241 AFLPVGSDRDHAAANVFGPKGEDISRRKFPATLILVGGFDLLCDRQRMYEEWLKRSGKKV 300

Query: 301 ELVEFSNAIHGFSGLPDLPEYSSMIREMKNFMERAPKF 339
           ELVEFSNAIHGFSGLPDLPEYSSMIREMKNFMERAPKF
Sbjct: 301 ELVEFSNAIHGFSGLPDLPEYSSMIREMKNFMERAPKF 338

BLAST of Moc11g27710 vs. ExPASy TrEMBL
Match: A0A6J1ECC8 (probable carboxylesterase 18 OS=Cucurbita moschata OX=3662 GN=LOC111432777 PE=3 SV=1)

HSP 1 Score: 446.0 bits (1146), Expect = 1.4e-121
Identity = 221/334 (66.17%), Postives = 258/334 (77.25%), Query Frame = 0

Query: 1   MASSSCSSEVSNRPQLPWKYRVMLRFAPLFFHIAFRSDSTVNRRLLQFVDRKAPASSEPL 60
           M  SS SSE+S   QLPWK+R+ LRFA L F+ + RSD TVNRRLL F+D K P +   +
Sbjct: 1   MQPSSVSSEIST-SQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSI 60

Query: 61  DGISSSDLTIDTSRSLWIRIYTPTAHGGDSPKLPVVLFFHGGGFALFHADTLACDVAARR 120
             +SSSDLTID SR L++RI+TPT    DS   PV+ +FHGGGFA   AD  + D+AARR
Sbjct: 61  HAVSSSDLTIDDSRDLFLRIFTPTVAATDSVLPPVIFYFHGGGFAFGSADATSMDMAARR 120

Query: 121 YAGELRAVVISVNYRLAPEFRFPSPYDDGFDALKFLDEI-DDGVLPAAADLGRCFILGES 180
           ++ EL AVVISVNYRLAP+FRFP  YDDGFDA+KF+DE+ DD  L   ADL RCFILGES
Sbjct: 121 FSKELGAVVISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSRCFILGES 180

Query: 181 AGGNLGHHVAVRASEYAFKKVTVAGFVAVQPFFGGEERTKSEIRLCNQPPFTREMADWFW 240
           AGGNLGHHVAVRASEYAFK+V V GF+A QPFFGGEERT+SEIRLCNQPP T ++ADWFW
Sbjct: 181 AGGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFW 240

Query: 241 KAFLPVGSDRDHAAANVFGPKGEDIS-RRKFPATLILVGGFDLLCDRQRMYEEWLKRSGK 300
           KAFLP G DRDHAAANV GP G D+S  R FPAT+IL GG DLL DRQR Y E LKR GK
Sbjct: 241 KAFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDLLIDRQRRYYEELKRMGK 300

Query: 301 KVELVEFSNAIHGFSGLPDLPEYSSMIREMKNFM 333
           +V+LV F+N +HGF    D PEYS MI+EM++F+
Sbjct: 301 EVKLVVFTNGVHGFFSFEDPPEYSLMIKEMRDFI 333

BLAST of Moc11g27710 vs. ExPASy TrEMBL
Match: A0A6J1IB87 (probable carboxylesterase 18 OS=Cucurbita maxima OX=3661 GN=LOC111471943 PE=4 SV=1)

HSP 1 Score: 444.5 bits (1142), Expect = 3.9e-121
Identity = 213/319 (66.77%), Postives = 254/319 (79.62%), Query Frame = 0

Query: 15  QLPWKYRVMLRFAPLFFHIAFRSDSTVNRRLLQFVDRKAPASSEPLDGISSSDLTIDTSR 74
           +LPWK+R+MLR   +  +   RSD T+NR L   +DR+ P SS+P+DG+SSSDL ID SR
Sbjct: 13  RLPWKHRLMLRLGTIATNTFCRSDFTINRWLTGILDRRTPPSSKPIDGVSSSDLLIDKSR 72

Query: 75  SLWIRIYTPTAHGGDSPKLPVVLFFHGGGFALFHADTLACDVAARRYAGELRAVVISVNY 134
           +LW+RI+TP     D   LPV+ ++HGGGFA  +AD+      A ++A +LRAVVISVNY
Sbjct: 73  NLWVRIFTPI---DDVQSLPVIFYYHGGGFAFSYADSSLYHTTAHQFAKQLRAVVISVNY 132

Query: 135 RLAPEFRFPSPYDDGFDALKFLDEIDDGVLPAAADLGRCFILGESAGGNLGHHVAVRASE 194
           RLAPEFRFP  YDDGFDALKF+DEIDDG LPA+ADLGR FILGESAGGNLGHHVAVRASE
Sbjct: 133 RLAPEFRFPCQYDDGFDALKFIDEIDDGTLPASADLGRSFILGESAGGNLGHHVAVRASE 192

Query: 195 YAFKKVTVAGFVAVQPFFGGEERTKSEIRLCNQPPFTREMADWFWKAFLPVGSDRDHAAA 254
           YAFKK+ + GF+A QPF G EERT+SEIRLCNQPP + ++ DWFWKAFLPVGSDRDHAAA
Sbjct: 193 YAFKKLKLIGFIATQPFIGAEERTESEIRLCNQPPLSLKLTDWFWKAFLPVGSDRDHAAA 252

Query: 255 NVFGPKGEDISR-RKFPATLILVGGFDLLCDRQRMYEEWLKRSGKKVELVEFSNAIHGFS 314
           NVFGP G DIS   KFPAT++LVGGFDLL D QR Y E LKR GK+V++VE  NAIHGF 
Sbjct: 253 NVFGPNGRDISGVEKFPATVVLVGGFDLLQDGQRRYYEGLKRMGKEVKMVELPNAIHGFF 312

Query: 315 GLPDLPEYSSMIREMKNFM 333
             PDLPEYSSMI+ +++F+
Sbjct: 313 CFPDLPEYSSMIQAVRDFI 328

BLAST of Moc11g27710 vs. ExPASy TrEMBL
Match: A0A6J1EWT7 (probable carboxylesterase 18 OS=Cucurbita moschata OX=3662 GN=LOC111438887 PE=4 SV=1)

HSP 1 Score: 440.3 bits (1131), Expect = 7.4e-120
Identity = 212/319 (66.46%), Postives = 253/319 (79.31%), Query Frame = 0

Query: 15  QLPWKYRVMLRFAPLFFHIAFRSDSTVNRRLLQFVDRKAPASSEPLDGISSSDLTIDTSR 74
           +LPWK+R+MLR   +  +   RSD T+NR L   +DR+ P SS P+DG+SSSDL ID SR
Sbjct: 13  RLPWKHRLMLRLGTIATNTFCRSDFTINRWLTGILDRRTPPSSIPIDGVSSSDLLIDPSR 72

Query: 75  SLWIRIYTPTAHGGDSPKLPVVLFFHGGGFALFHADTLACDVAARRYAGELRAVVISVNY 134
           +LW+RI+TP         LPV+ ++HGGGFA  +AD+      A ++A +LRAVVISVNY
Sbjct: 73  NLWVRIFTPI---DAVQSLPVIFYYHGGGFAFSYADSSLYHTTAHQFAKQLRAVVISVNY 132

Query: 135 RLAPEFRFPSPYDDGFDALKFLDEIDDGVLPAAADLGRCFILGESAGGNLGHHVAVRASE 194
           RLAPEFRFP  YDDGFDALKF+DEIDDG LPA+ADLGR FILGESAGGNLGHHVAVRASE
Sbjct: 133 RLAPEFRFPCQYDDGFDALKFIDEIDDGTLPASADLGRSFILGESAGGNLGHHVAVRASE 192

Query: 195 YAFKKVTVAGFVAVQPFFGGEERTKSEIRLCNQPPFTREMADWFWKAFLPVGSDRDHAAA 254
           YAFKK+ + GF+A QPFFG EERT+SEIRLCNQ P +  ++DWFWKAFLPVGSDRDHAAA
Sbjct: 193 YAFKKLKLIGFIATQPFFGAEERTESEIRLCNQAPLSLRLSDWFWKAFLPVGSDRDHAAA 252

Query: 255 NVFGPKGEDISR-RKFPATLILVGGFDLLCDRQRMYEEWLKRSGKKVELVEFSNAIHGFS 314
           NVFGP G DIS   +FPAT++LVGGFDLL D QR Y E LKR GK+V++VE  NAIHGF 
Sbjct: 253 NVFGPNGRDISGVERFPATVVLVGGFDLLQDGQRRYYEGLKRMGKEVKMVELPNAIHGFF 312

Query: 315 GLPDLPEYSSMIREMKNFM 333
             PDLPEYSSMI+E+++F+
Sbjct: 313 CFPDLPEYSSMIQEVRDFI 328

BLAST of Moc11g27710 vs. ExPASy TrEMBL
Match: A0A6J1IER7 (probable carboxylesterase 18 OS=Cucurbita maxima OX=3661 GN=LOC111472040 PE=3 SV=1)

HSP 1 Score: 439.9 bits (1130), Expect = 9.7e-120
Identity = 218/334 (65.27%), Postives = 255/334 (76.35%), Query Frame = 0

Query: 1   MASSSCSSEVSNRPQLPWKYRVMLRFAPLFFHIAFRSDSTVNRRLLQFVDRKAPASSEPL 60
           M   S SSE+S   QLPWK+R+ LRFA L F+ + RSD TVNRRLL F+D K P +   +
Sbjct: 1   MQPFSVSSEIST-SQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSI 60

Query: 61  DGISSSDLTIDTSRSLWIRIYTPTAHGGDSPKLPVVLFFHGGGFALFHADTLACDVAARR 120
             +SSSDLTID SR L++RI+TPT    DS   PV+ +FHGGGFA   A   + D+AARR
Sbjct: 61  HAVSSSDLTIDASRDLFLRIFTPTVAATDSLLPPVIFYFHGGGFAFGSAAATSMDMAARR 120

Query: 121 YAGELRAVVISVNYRLAPEFRFPSPYDDGFDALKFLDEIDD-GVLPAAADLGRCFILGES 180
           ++ EL AVVISVNYRL+P+FRFP  YDDGFDA+KF+DE+DD   L   ADL RCFILGES
Sbjct: 121 FSKELGAVVISVNYRLSPQFRFPCQYDDGFDAVKFIDEMDDEDPLLGRADLSRCFILGES 180

Query: 181 AGGNLGHHVAVRASEYAFKKVTVAGFVAVQPFFGGEERTKSEIRLCNQPPFTREMADWFW 240
           AGGNLGHHVAVRASEYAFK+V V GF+A QPFFGGEERT+SEIRLCNQPP T ++ADWFW
Sbjct: 181 AGGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFW 240

Query: 241 KAFLPVGSDRDHAAANVFGPKGEDIS-RRKFPATLILVGGFDLLCDRQRMYEEWLKRSGK 300
           KAFLP G DRDHAAANV GP G D+S  R FPAT+IL GG D L DRQR Y E LKR GK
Sbjct: 241 KAFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDFLIDRQRRYYEELKRMGK 300

Query: 301 KVELVEFSNAIHGFSGLPDLPEYSSMIREMKNFM 333
            V+LV F+N +HGF    DLPEYS MI+EM++F+
Sbjct: 301 DVKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFI 333

BLAST of Moc11g27710 vs. TAIR 10
Match: AT5G23530.1 (carboxyesterase 18 )

HSP 1 Score: 311.2 bits (796), Expect = 1.0e-84
Identity = 154/329 (46.81%), Postives = 208/329 (63.22%), Query Frame = 0

Query: 8   SEVSNRPQLPWKYRVMLRFAPLFFHIAFRSDSTVNRRLLQFVDRKAPASSEPLDGISSSD 67
           S+ + +  LP K R+ L         A R D T+NRR L+  D +AP + +P++ +S+SD
Sbjct: 5   SQPNQKLTLPLKTRIALTVISTMTDNAQRPDGTINRRFLRLFDFRAPPNPKPVNIVSTSD 64

Query: 68  LTIDTSRSLWIRIYTPTAHGGDSPKLPVVLFFHGGGFALFHADTLACDVAARRYAGELRA 127
             +D SR LW R+YTP   G    K+PVV+FFHGGGFA    +    D   RR+A +L A
Sbjct: 65  FVVDQSRDLWFRLYTPHVSG---DKIPVVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPA 124

Query: 128 VVISVNYRLAPEFRFPSPYDDGFDALKFLDEIDDGVLPAAADLGRCFILGESAGGNLGHH 187
            VISVNYRLAPE R+P+ YDDGFDALK+++E    +LPA ADL RCF  G+SAGGN+ H+
Sbjct: 125 YVISVNYRLAPEHRYPAQYDDGFDALKYIEENHGSILPANADLSRCFFAGDSAGGNIAHN 184

Query: 188 VAV---RASEYAFKKVTVAGFVAVQPFFGGEERTKSEIRLCNQPPFTREMADWFWKAFLP 247
           VA+   R    +F  V + G +++QPFFGGEERT++E +L   P  + +  DW WKA   
Sbjct: 185 VAIRICREPRSSFTAVKLIGLISIQPFFGGEERTEAEKQLVGAPLVSPDRTDWCWKA--- 244

Query: 248 VGSDRDHAAANVFGPKGEDISRRKFPATLILVGGFDLLCDRQRMYEEWLKRSGKKVELVE 307
           +G +RDH A NV GP   DIS   +P T+++V GFD L D QR Y EWLK  GKK  L+E
Sbjct: 245 MGLNRDHEAVNVGGPNAVDISGLDYPETMVVVAGFDPLKDWQRSYYEWLKLCGKKATLIE 304

Query: 308 FSNAIHGFSGLPDLPEYSSMIREMKNFME 334
           + N  H F   P+LPE   +I  +K+F++
Sbjct: 305 YPNMFHAFYIFPELPEAGQLIMRIKDFVD 327

BLAST of Moc11g27710 vs. TAIR 10
Match: AT5G27320.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 239.6 bits (610), Expect = 3.7e-63
Identity = 131/344 (38.08%), Postives = 198/344 (57.56%), Query Frame = 0

Query: 1   MASSSCSSEVSNRPQLPWKYRVMLRFAPLFFHIAFRSDSTVNRRLLQFVDRKAPASSEPL 60
           MA S   + + ++  +P    V++    L +++  R D T NR L +F+DRK PA++ P+
Sbjct: 1   MAGSEEVNLIESKTVVPLNTWVLISNFKLAYNLLRRPDGTFNRHLAEFLDRKVPANANPV 60

Query: 61  DGISSSDLTIDTSRSLWIRIYTPTAHGGDSPKL------------PVVLFFHGGGFALFH 120
           +G+ S D+ ID   +L  R+Y P A  G SP +            PV++FFHGG FA   
Sbjct: 61  NGVFSFDVIIDRQTNLLSRVYRP-ADAGTSPSITDLQNPVDGEIVPVIVFFHGGSFAHSS 120

Query: 121 ADTLACDVAARRYAGELRAVVISVNYRLAPEFRFPSPYDDGFDALKFLDEIDDGVLPAAA 180
           A++   D   RR  G   AVV+SVNYR APE R+P  YDDG+  LK+++     +     
Sbjct: 121 ANSAIYDTLCRRLVGLCGAVVVSVNYRRAPENRYPCAYDDGWAVLKWVNS-SSWLRSKKD 180

Query: 181 DLGRCFILGESAGGNLGHHVAVRASEYAFKKVTVAGFVAVQPFFGGEERTKSEIRLCNQP 240
              R F+ G+S+GGN+ H+VAVRA E    ++ V G + + P FGG ERT+SE RL  + 
Sbjct: 181 SKVRIFLAGDSSGGNIVHNVAVRAVE---SRIDVLGNILLNPMFGGTERTESEKRLDGKY 240

Query: 241 PFTREMADWFWKAFLPVGSDRDHAAANVFGPKGEDISRRKFPATLILVGGFDLLCDRQRM 300
             T    DW+W+AFLP G DR+H A + FGP+ + +    FP +L++V G DL+ D Q  
Sbjct: 241 FVTVRDRDWYWRAFLPEGEDREHPACSPFGPRSKSLEGLSFPKSLVVVAGLDLIQDWQLK 300

Query: 301 YEEWLKRSGKKVELVEFSNAIHGFSGLPDLPEYSSMIREMKNFM 333
           Y E LK++G++V+L+    A  GF  LP+   + +++ E+  F+
Sbjct: 301 YAEGLKKAGQEVKLLYLEQATIGFYLLPNNNHFHTVMDEIAAFV 339

BLAST of Moc11g27710 vs. TAIR 10
Match: AT3G05120.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 230.7 bits (587), Expect = 1.7e-60
Identity = 128/346 (36.99%), Postives = 194/346 (56.07%), Query Frame = 0

Query: 1   MASSSCSSEVSNRPQLPWKYRVMLRFAPLFFHIAFRSDSTVNRRLLQFVDRKAPASSEPL 60
           MA+S   + + +R  +P    V++    + ++I  R D T NR L +++DRK  A++ P+
Sbjct: 1   MAASDEVNLIESRTVVPLNTWVLISNFKVAYNILRRPDGTFNRHLAEYLDRKVTANANPV 60

Query: 61  DGISSSDLTIDTSRSLWIRIYTPTAHGGDSPK-------------LPVVLFFHGGGFALF 120
           DG+ S D+ ID   +L  R+Y P     + P              +PV+LFFHGG FA  
Sbjct: 61  DGVFSFDVLIDRRINLLSRVYRPAYADQEQPPSILDLEKPVDGDIVPVILFFHGGSFAHS 120

Query: 121 HADTLACDVAARRYAGELRAVVISVNYRLAPEFRFPSPYDDGFDALKFLDEIDDGVLPAA 180
            A++   D   RR  G  + VV+SVNYR APE  +P  YDDG+ AL +++      L + 
Sbjct: 121 SANSAIYDTLCRRLVGLCKCVVVSVNYRRAPENPYPCAYDDGWIALNWVN--SRSWLKSK 180

Query: 181 ADLG-RCFILGESAGGNLGHHVAVRASEYAFKKVTVAGFVAVQPFFGGEERTKSEIRLCN 240
            D     F+ G+S+GGN+ H+VA+RA E     + V G + + P FGG ERT+SE  L  
Sbjct: 181 KDSKVHIFLAGDSSGGNIAHNVALRAGE---SGIDVLGNILLNPMFGGNERTESEKSLDG 240

Query: 241 QPPFTREMADWFWKAFLPVGSDRDHAAANVFGPKGEDISRRKFPATLILVGGFDLLCDRQ 300
           +   T    DW+WKAFLP G DR+H A N F P+G+ +    FP +L++V G DL+ D Q
Sbjct: 241 KYFVTVRDRDWYWKAFLPEGEDREHPACNPFSPRGKSLEGVSFPKSLVVVAGLDLIRDWQ 300

Query: 301 RMYEEWLKRSGKKVELVEFSNAIHGFSGLPDLPEYSSMIREMKNFM 333
             Y E LK++G++V+L+    A  GF  LP+   + +++ E+  F+
Sbjct: 301 LAYAEGLKKAGQEVKLMHLEKATVGFYLLPNNNHFHNVMDEISAFV 341

BLAST of Moc11g27710 vs. TAIR 10
Match: AT3G63010.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 219.5 bits (558), Expect = 4.0e-57
Identity = 124/332 (37.35%), Postives = 188/332 (56.63%), Query Frame = 0

Query: 16  LPWKYRVMLRFAPLFFHIAFRSDSTVNRRLLQFVDRKAPASSEPLDGISSSDLTIDTSRS 75
           +P    V++    L + +  R D + NR L +F+DRK PA+S PLDG+ S D  +D++ +
Sbjct: 16  VPLNTWVLISNFKLAYKVLRRPDGSFNRDLAEFLDRKVPANSFPLDGVFSFD-HVDSTTN 75

Query: 76  LWIRIYTP------TAHG--------GDSPKLPVVLFFHGGGFALFHADTLACDVAARRY 135
           L  RIY P      T HG          +  +PV++FFHGG F    A++   D   RR 
Sbjct: 76  LLTRIYQPASLLHQTRHGTLELTKPLSTTEIVPVLIFFHGGSFTHSSANSAIYDTFCRRL 135

Query: 136 AGELRAVVISVNYRLAPEFRFPSPYDDGFDALKFLDEIDDGVLPAAADLG-RCFILGESA 195
                 VV+SV+YR +PE R+P  YDDG++AL ++       L +  D     ++ G+S+
Sbjct: 136 VTICGVVVVSVDYRRSPEHRYPCAYDDGWNALNWVK--SRVWLQSGKDSNVYVYLAGDSS 195

Query: 196 GGNLGHHVAVRASEYAFKKVTVAGFVAVQPFFGGEERTKSEIRLCNQPPFTREMADWFWK 255
           GGN+ H+VAVRA+    + V V G + + P FGG+ERT+SE  L  +   T +  DW+W+
Sbjct: 196 GGNIAHNVAVRATN---EGVKVLGNILLHPMFGGQERTQSEKTLDGKYFVTIQDRDWYWR 255

Query: 256 AFLPVGSDRDHAAANVFGPKGEDISRRKFPATLILVGGFDLLCDRQRMYEEWLKRSGKKV 315
           A+LP G DRDH A N FGP+G+ +    FP +L++V G DL+ D Q  Y + LK++G +V
Sbjct: 256 AYLPEGEDRDHPACNPFGPRGQSLKGVNFPKSLVVVAGLDLVQDWQLAYVDGLKKTGLEV 315

Query: 316 ELVEFSNAIHGFSGLPDLPEYSSMIREMKNFM 333
            L+    A  GF  LP+   +  ++ E+  F+
Sbjct: 316 NLLYLKQATIGFYFLPNNDHFHCLMEELNKFV 341

BLAST of Moc11g27710 vs. TAIR 10
Match: AT5G62180.1 (carboxyesterase 20 )

HSP 1 Score: 179.1 bits (453), Expect = 5.9e-45
Identity = 108/315 (34.29%), Postives = 167/315 (53.02%), Query Frame = 0

Query: 30  FFHIAFRSDSTVNRRLLQFVDRKAPASSEPLDGISSSDLTIDTSRSLWIRIYTPTAHGGD 89
           + +I    D ++ R L  F    A     PL+   S DL ++  +S W+R+Y P++   +
Sbjct: 13  YLNIVNNPDGSITRDLSNFPCTAATPDPSPLNPAVSKDLPVNQLKSTWLRLYLPSSAVNE 72

Query: 90  ----SPKLPVVLFFHGGGFALFHADTLACDVAARRYAGELRAVVISVNYRLAPEFRFPSP 149
               S KLP+V+++HGGGF L   D           A +L A+V+S +YRLAPE R P+ 
Sbjct: 73  GNVSSQKLPIVVYYHGGGFILCSVDMQLFHDFCSEVARDLNAIVVSPSYRLAPEHRLPAA 132

Query: 150 YDDGFDALKFLDEIDDGVLPAAADLGRCFILGESAGGNLGHHVAVRA--SEYAFKKVTVA 209
           YDDG +AL ++   DD  + + AD    F++G SAGGNL ++V +R+  S      + + 
Sbjct: 133 YDDGVEALDWIKTSDDEWIKSHADFSNVFLMGTSAGGNLAYNVGLRSVDSVSDLSPLQIR 192

Query: 210 GFVAVQPFFGGEERTKSEIRLCNQPPFTREMADWFWKAFLPVGSDRDHAAANVF----GP 269
           G +   PFFGGEER++SEIRL N       + D  W   LPVG DRDH  +N        
Sbjct: 193 GLILHHPFFGGEERSESEIRLMNDQVCPPIVTDVMWDLSLPVGVDRDHEYSNPTVGDGSE 252

Query: 270 KGEDISRRKFPATLILVGGFDLLCDRQRMYEEWLKRSGKKVELVEF--SNAIHGFSGLPD 329
           K E I R ++   +++ G  D + D Q+   + +K+ G  VE+VE      +HG + + D
Sbjct: 253 KLEKIGRLRW-KVMMIGGEDDPMIDLQKDVAKLMKKKG--VEVVEHYTGGHVHG-AEIRD 312

Query: 330 LPEYSSMIREMKNFM 333
             +  ++   +KNF+
Sbjct: 313 PSKRKTLFLSIKNFI 323

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022159210.12.0e-19699.70probable carboxylesterase 18 [Momordica charantia][more]
XP_022925491.12.8e-12166.17probable carboxylesterase 18 [Cucurbita moschata][more]
XP_022973390.18.1e-12166.77probable carboxylesterase 18 [Cucurbita maxima][more]
KAG6607539.18.1e-12167.40putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG6592331.13.1e-12065.57putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. sororia] >K... [more]
Match NameE-valueIdentityDescription
Q9LT101.4e-8346.81Probable carboxylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=CXE18 PE=1 SV=1[more]
Q940G65.2e-6238.08Gibberellin receptor GID1C OS=Arabidopsis thaliana OX=3702 GN=GID1C PE=1 SV=1[more]
Q6L5455.2e-6238.24Gibberellin receptor GID1 OS=Oryza sativa subsp. japonica OX=39947 GN=GID1 PE=1 ... [more]
Q9MAA72.4e-5936.99Gibberellin receptor GID1A OS=Arabidopsis thaliana OX=3702 GN=GID1A PE=1 SV=1[more]
Q9LYC15.6e-5637.35Gibberellin receptor GID1B OS=Arabidopsis thaliana OX=3702 GN=GID1B PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1DY159.8e-19799.70probable carboxylesterase 18 OS=Momordica charantia OX=3673 GN=LOC111025630 PE=4... [more]
A0A6J1ECC81.4e-12166.17probable carboxylesterase 18 OS=Cucurbita moschata OX=3662 GN=LOC111432777 PE=3 ... [more]
A0A6J1IB873.9e-12166.77probable carboxylesterase 18 OS=Cucurbita maxima OX=3661 GN=LOC111471943 PE=4 SV... [more]
A0A6J1EWT77.4e-12066.46probable carboxylesterase 18 OS=Cucurbita moschata OX=3662 GN=LOC111438887 PE=4 ... [more]
A0A6J1IER79.7e-12065.27probable carboxylesterase 18 OS=Cucurbita maxima OX=3661 GN=LOC111472040 PE=3 SV... [more]
Match NameE-valueIdentityDescription
AT5G23530.11.0e-8446.81carboxyesterase 18 [more]
AT5G27320.13.7e-6338.08alpha/beta-Hydrolases superfamily protein [more]
AT3G05120.11.7e-6036.99alpha/beta-Hydrolases superfamily protein [more]
AT3G63010.14.0e-5737.35alpha/beta-Hydrolases superfamily protein [more]
AT5G62180.15.9e-4534.29carboxyesterase 20 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013094Alpha/beta hydrolase fold-3PFAMPF07859Abhydrolase_3coord: 96..312
e-value: 1.3E-52
score: 178.8
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 1..333
e-value: 3.8E-89
score: 301.3
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 41..334
NoneNo IPR availablePANTHERPTHR23024ARYLACETAMIDE DEACETYLASEcoord: 12..334
NoneNo IPR availablePANTHERPTHR23024:SF434ACETYL ESTERASEcoord: 12..334

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc11g27710.1Moc11g27710.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity