Moc11g25770 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc11g25770
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionRetrovirus-related Pol polyprotein from transposon RE1
Locationchr11: 18866785 .. 18868479 (+)
RNA-Seq ExpressionMoc11g25770
SyntenyMoc11g25770
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTCGGGCGGCGGGACGGTAGATCTAGTTTGGGACGTGGCGCATCAAGCACATATGGTACGGGTGGCGGCCAACGGGGAGGGTGGCGGCGGTTGGGTGCGACGGTGACCGATATAATTTTGGTTCGTGGACGGTGGCGTGAAGGTGAGCGGCGCATGGATCTGCTTCGTGAGGTGAGCAGCAAGACAGGGTGGTGGCCTTAACGGTGAGAGGCGGGATGGCGGCTTGCGGATCTACTTCGTGAGGAAGACGATGGTTGTAGGGTGCATGAAGTTGCGTGAGACCAAAAGGTGGGAAGGGACTCCTAAAACCCTAACTTCTCTAACTCAAGTCAAAGGTGAAAAGTAAATTTTATATTTACTTAGTAATAATCTTATTTGTCCTCTTATTTTGGAATTTTAGAGTGGTAAATTTCAAAATCTACCCTAATACATTTTTAATAAATTTTTCTTAAATCAATTTTCAAATTCTGATTATCAATTTTAAATTAGATTTAAGTTAACTATTTAAATTTCATTCATTCGATTAGATAGAATCGATTCTCAAATGAAAGCCCGTTATTTTTGCCTAAAATTATACGTATATACTTAATTGGTTACAAGGTTATATTTGTCTACAAAATATTTTAGAATTTAACGGAAAAAATCAATTAGCCATTATTGCAATATTTCATAGGTTTGGACATTTTTTAAAACGCGTTATTAATTTTAGTCATTCCTGCAATTGCCCCGATTAAGATTAATCATTGATGGGTTTTAATAATAAATGTGTTTTAGTGATATTGGGCTTTAGTTACACTTTGTTTTATATGTAACCATTATTTTGTCATCTGTTGAAAGTTGTTAAGAGCCCAATAGTCTATATAAGAACGTTAACACCTCCTGTATTTTGTAATGGATTTCAATACAAAATAATCCATTATGTTACGATGGTATCAGAGCTCTCAAACGTCTAACGACGATTCAAGAAATATGACAGACTCTATAGAAGAGAATTCTGAAGTCACTACGAGCCAAGGATTCACGTTGATCAATCCGGGCAATAAAATCAATACAGTAAAGCTGACAGACAACAATTTCCTATTATGGAAGTTTCAGGTACTAGCAGCCTTAGAAGGTAATGGTTTAGAGAGCTATATTGATGAAAATAATCAAATTCCACAGCAGTAAATTGTCAGTACAGAAAACTCGATAATTACACAAAGGCCAAACCTAGCCTATACGCAGTGGAAAAGACAAGACAAACTGATAATCTCTTGGCTTCTGGGATCCATGTCAGAGGAGATTCTTGCAGACCTTCTTCACTACAAAACAACGCAAGAAATATGGAGCAATCTTCATCAAATCTTTACTACAAGAAATCTTGCCAAAGTAATGGAAATTTGAACTAGACTTCAGAATATCAAGAAAGGTAATTCTACTATTAAAGACTATGTTCGACAAGTTAAGAGCTTAGTTGATTCTTTACTAGCTACGGGACAACTAGTCACACCAGAGGATCACGTTCTTTACATTCTATCCGGATTAGGATTAGAATATGAGTCAACCATTTCAGTAATTACAACACAATCAGGTGAAGTTTCAGTACAAAAAGCTACTTCATTACTACTATCTCATGAAAGTAGAATGGATAAAGATGTGGCTCAAACAAATGTAAAGGATTCAACACTACCATCAGTAAATCTTGTTCAATAG

mRNA sequence

ATGGTCGGGCGGCGGGACGGTAGATCTAGTTTGGGACGTGGCGCATCAAGCACATATGGTACGGGTGGCGGCCAACGGGGAGGGTGGCGGCGGTTGGGTGCGACGGTGACCGATATAATTTTGGTTCGTGGACGGTGGCGTGAAGGTGAGCGGCGCATGGATCTGCTTCGTGAGAGCTCTCAAACGTCTAACGACGATTCAAGAAATATGACAGACTCTATAGAAGAGAATTCTGAAGTCACTACGAGCCAAGGATTCACGTTGATCAATCCGGGCAATAAAATCAATACAGTAAAGCTGACAGACAACAATTTCCTATTATGGAAGTTTCAGAATATCAAGAAAGGTAATTCTACTATTAAAGACTATGTTCGACAAGTTAAGAGCTTAGTTGATTCTTTACTAGCTACGGGACAACTAGTCACACCAGAGGATCACGTTCTTTACATTCTATCCGGATTAGGATTAGAATATGAGTCAACCATTTCAGTAATTACAACACAATCAGGTGAAGTTTCAGTACAAAAAGCTACTTCATTACTACTATCTCATGAAAGTAGAATGGATAAAGATGTGGCTCAAACAAATGTAAAGGATTCAACACTACCATCAGTAAATCTTGTTCAATAG

Coding sequence (CDS)

ATGGTCGGGCGGCGGGACGGTAGATCTAGTTTGGGACGTGGCGCATCAAGCACATATGGTACGGGTGGCGGCCAACGGGGAGGGTGGCGGCGGTTGGGTGCGACGGTGACCGATATAATTTTGGTTCGTGGACGGTGGCGTGAAGGTGAGCGGCGCATGGATCTGCTTCGTGAGAGCTCTCAAACGTCTAACGACGATTCAAGAAATATGACAGACTCTATAGAAGAGAATTCTGAAGTCACTACGAGCCAAGGATTCACGTTGATCAATCCGGGCAATAAAATCAATACAGTAAAGCTGACAGACAACAATTTCCTATTATGGAAGTTTCAGAATATCAAGAAAGGTAATTCTACTATTAAAGACTATGTTCGACAAGTTAAGAGCTTAGTTGATTCTTTACTAGCTACGGGACAACTAGTCACACCAGAGGATCACGTTCTTTACATTCTATCCGGATTAGGATTAGAATATGAGTCAACCATTTCAGTAATTACAACACAATCAGGTGAAGTTTCAGTACAAAAAGCTACTTCATTACTACTATCTCATGAAAGTAGAATGGATAAAGATGTGGCTCAAACAAATGTAAAGGATTCAACACTACCATCAGTAAATCTTGTTCAATAG

Protein sequence

MVGRRDGRSSLGRGASSTYGTGGGQRGGWRRLGATVTDIILVRGRWREGERRMDLLRESSQTSNDDSRNMTDSIEENSEVTTSQGFTLINPGNKINTVKLTDNNFLLWKFQNIKKGNSTIKDYVRQVKSLVDSLLATGQLVTPEDHVLYILSGLGLEYESTISVITTQSGEVSVQKATSLLLSHESRMDKDVAQTNVKDSTLPSVNLVQ
Homology
BLAST of Moc11g25770 vs. NCBI nr
Match: XP_022158089.1 (uncharacterized protein LOC111024658 [Momordica charantia])

HSP 1 Score: 107.5 bits (267), Expect = 1.5e-19
Identity = 54/111 (48.65%), Postives = 82/111 (73.87%), Query Frame = 0

Query: 97  TVKLTDNNFLLWKFQNIKKGNSTIKDYVRQVKSLVDSLLATGQLVTPEDHVLYILSGLGL 156
           T  LT    L  + QN++KG  ++K+Y+RQ+K+LVDSL A G+ +  EDH+++ILSGLG 
Sbjct: 28  TQNLTKVMQLKTQLQNLRKGGLSLKEYIRQIKNLVDSLKAAGKSMNTEDHIMHILSGLGS 87

Query: 157 EYESTISVITTQSGEVSVQKATSLLLSHESRMDKDVAQTNVKDSTLPSVNL 208
           EYEST+SVIT + G +++Q  T+LLLSH+ R++K+++    KD TLPS ++
Sbjct: 88  EYESTVSVITVKKGPLTIQDVTALLLSHQRRIEKEISA--AKDVTLPSASI 136

BLAST of Moc11g25770 vs. NCBI nr
Match: XP_022136882.1 (dr1-associated corepressor homolog isoform X1 [Momordica charantia])

HSP 1 Score: 101.3 bits (251), Expect = 1.0e-17
Identity = 54/101 (53.47%), Postives = 75/101 (74.26%), Query Frame = 0

Query: 109 KFQNIKKGNSTIKDYVRQVKSLVDSLLATGQLVTPEDHVLYILSGLGLEYESTISVITTQ 168
           K +NIKKGN  +KDY ++VK+LVDSL A G+ VT EDH+++IL+GL  E+EST+SVI+ +
Sbjct: 54  KLENIKKGNLPLKDYFQKVKALVDSLAAAGKKVTVEDHIMHILTGLRSEFESTVSVISAR 113

Query: 169 SGEVSVQKATSLLLSHESRMDKDVAQTNVKDSTLPSVNLVQ 210
           +   ++Q+  SLLLSHE R +++   T   D TLPSVNL Q
Sbjct: 114 TQTQTLQEVYSLLLSHEGRNERNSINT---DGTLPSVNLTQ 151

BLAST of Moc11g25770 vs. NCBI nr
Match: XP_022136883.1 (dr1-associated corepressor homolog isoform X2 [Momordica charantia])

HSP 1 Score: 101.3 bits (251), Expect = 1.0e-17
Identity = 54/101 (53.47%), Postives = 75/101 (74.26%), Query Frame = 0

Query: 109 KFQNIKKGNSTIKDYVRQVKSLVDSLLATGQLVTPEDHVLYILSGLGLEYESTISVITTQ 168
           K +NIKKGN  +KDY ++VK+LVDSL A G+ VT EDH+++IL+GL  E+EST+SVI+ +
Sbjct: 54  KLENIKKGNLPLKDYFQKVKALVDSLAAAGKKVTVEDHIMHILTGLRSEFESTVSVISAR 113

Query: 169 SGEVSVQKATSLLLSHESRMDKDVAQTNVKDSTLPSVNLVQ 210
           +   ++Q+  SLLLSHE R +++   T   D TLPSVNL Q
Sbjct: 114 TQTQTLQEVYSLLLSHEGRNERNSINT---DGTLPSVNLTQ 151

BLAST of Moc11g25770 vs. NCBI nr
Match: XP_022154487.1 (uncharacterized protein LOC111021757 [Momordica charantia])

HSP 1 Score: 86.3 bits (212), Expect = 3.5e-13
Identity = 44/99 (44.44%), Postives = 71/99 (71.72%), Query Frame = 0

Query: 109 KFQNIKKGNSTIKDYVRQVKSLVDSLLATGQLVTPEDHVLYILSGLGLEYESTISVITTQ 168
           K +N KKGN ++KDY  ++K+LVDSL   G+ ++ EDH+++IL+GLG E+++ ISVIT +
Sbjct: 144 KLENFKKGNLSLKDYFLKIKNLVDSLAIAGKKLSTEDHIMHILAGLGPEFDAIISVITAR 203

Query: 169 SGEVSVQKATSLLLSHESRMDKDVAQTNVKDSTLPSVNL 208
           +   ++Q+  SLLL  E R ++++  +   D +LPSVNL
Sbjct: 204 NMPQTLQEVCSLLLQQEGRNERNLINS---DGSLPSVNL 239

BLAST of Moc11g25770 vs. NCBI nr
Match: KAA0048297.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. makuwa])

HSP 1 Score: 80.5 bits (197), Expect = 1.9e-11
Identity = 42/100 (42.00%), Postives = 68/100 (68.00%), Query Frame = 0

Query: 109 KFQNIKKGNSTIKDYVRQVKSLVDSLLATGQLVTPEDHVLYILSGLGLEYESTISVITTQ 168
           K  NIKKG+  +K+Y  ++   VD+L +  + V+ +DH+LYIL+GLG +Y+S ISVI+ +
Sbjct: 145 KLHNIKKGSMPLKEYFLKILQCVDALASINKPVSSDDHILYILAGLGSDYQSMISVISAR 204

Query: 169 SGEVSVQKATSLLLSHESRMDKDVAQTNVKDSTLPSVNLV 209
           +   SVQ+  SLLL+ ES+ +  +    + ++ LPSVN+V
Sbjct: 205 TDSPSVQEVMSLLLTQESQNESKL----ISETALPSVNIV 240

BLAST of Moc11g25770 vs. ExPASy TrEMBL
Match: A0A6J1DYD5 (uncharacterized protein LOC111024658 OS=Momordica charantia OX=3673 GN=LOC111024658 PE=4 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 7.1e-20
Identity = 54/111 (48.65%), Postives = 82/111 (73.87%), Query Frame = 0

Query: 97  TVKLTDNNFLLWKFQNIKKGNSTIKDYVRQVKSLVDSLLATGQLVTPEDHVLYILSGLGL 156
           T  LT    L  + QN++KG  ++K+Y+RQ+K+LVDSL A G+ +  EDH+++ILSGLG 
Sbjct: 28  TQNLTKVMQLKTQLQNLRKGGLSLKEYIRQIKNLVDSLKAAGKSMNTEDHIMHILSGLGS 87

Query: 157 EYESTISVITTQSGEVSVQKATSLLLSHESRMDKDVAQTNVKDSTLPSVNL 208
           EYEST+SVIT + G +++Q  T+LLLSH+ R++K+++    KD TLPS ++
Sbjct: 88  EYESTVSVITVKKGPLTIQDVTALLLSHQRRIEKEISA--AKDVTLPSASI 136

BLAST of Moc11g25770 vs. ExPASy TrEMBL
Match: A0A6J1C8R2 (dr1-associated corepressor homolog isoform X2 OS=Momordica charantia OX=3673 GN=LOC111008464 PE=4 SV=1)

HSP 1 Score: 101.3 bits (251), Expect = 5.1e-18
Identity = 54/101 (53.47%), Postives = 75/101 (74.26%), Query Frame = 0

Query: 109 KFQNIKKGNSTIKDYVRQVKSLVDSLLATGQLVTPEDHVLYILSGLGLEYESTISVITTQ 168
           K +NIKKGN  +KDY ++VK+LVDSL A G+ VT EDH+++IL+GL  E+EST+SVI+ +
Sbjct: 54  KLENIKKGNLPLKDYFQKVKALVDSLAAAGKKVTVEDHIMHILTGLRSEFESTVSVISAR 113

Query: 169 SGEVSVQKATSLLLSHESRMDKDVAQTNVKDSTLPSVNLVQ 210
           +   ++Q+  SLLLSHE R +++   T   D TLPSVNL Q
Sbjct: 114 TQTQTLQEVYSLLLSHEGRNERNSINT---DGTLPSVNLTQ 151

BLAST of Moc11g25770 vs. ExPASy TrEMBL
Match: A0A6J1C6N9 (dr1-associated corepressor homolog isoform X1 OS=Momordica charantia OX=3673 GN=LOC111008464 PE=4 SV=1)

HSP 1 Score: 101.3 bits (251), Expect = 5.1e-18
Identity = 54/101 (53.47%), Postives = 75/101 (74.26%), Query Frame = 0

Query: 109 KFQNIKKGNSTIKDYVRQVKSLVDSLLATGQLVTPEDHVLYILSGLGLEYESTISVITTQ 168
           K +NIKKGN  +KDY ++VK+LVDSL A G+ VT EDH+++IL+GL  E+EST+SVI+ +
Sbjct: 54  KLENIKKGNLPLKDYFQKVKALVDSLAAAGKKVTVEDHIMHILTGLRSEFESTVSVISAR 113

Query: 169 SGEVSVQKATSLLLSHESRMDKDVAQTNVKDSTLPSVNLVQ 210
           +   ++Q+  SLLLSHE R +++   T   D TLPSVNL Q
Sbjct: 114 TQTQTLQEVYSLLLSHEGRNERNSINT---DGTLPSVNLTQ 151

BLAST of Moc11g25770 vs. ExPASy TrEMBL
Match: A0A6J1DLT9 (uncharacterized protein LOC111021757 OS=Momordica charantia OX=3673 GN=LOC111021757 PE=4 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 1.7e-13
Identity = 44/99 (44.44%), Postives = 71/99 (71.72%), Query Frame = 0

Query: 109 KFQNIKKGNSTIKDYVRQVKSLVDSLLATGQLVTPEDHVLYILSGLGLEYESTISVITTQ 168
           K +N KKGN ++KDY  ++K+LVDSL   G+ ++ EDH+++IL+GLG E+++ ISVIT +
Sbjct: 144 KLENFKKGNLSLKDYFLKIKNLVDSLAIAGKKLSTEDHIMHILAGLGPEFDAIISVITAR 203

Query: 169 SGEVSVQKATSLLLSHESRMDKDVAQTNVKDSTLPSVNL 208
           +   ++Q+  SLLL  E R ++++  +   D +LPSVNL
Sbjct: 204 NMPQTLQEVCSLLLQQEGRNERNLINS---DGSLPSVNL 239

BLAST of Moc11g25770 vs. ExPASy TrEMBL
Match: A0A5A7U233 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold264G00060 PE=4 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 9.2e-12
Identity = 42/100 (42.00%), Postives = 68/100 (68.00%), Query Frame = 0

Query: 109 KFQNIKKGNSTIKDYVRQVKSLVDSLLATGQLVTPEDHVLYILSGLGLEYESTISVITTQ 168
           K  NIKKG+  +K+Y  ++   VD+L +  + V+ +DH+LYIL+GLG +Y+S ISVI+ +
Sbjct: 145 KLHNIKKGSMPLKEYFLKILQCVDALASINKPVSSDDHILYILAGLGSDYQSMISVISAR 204

Query: 169 SGEVSVQKATSLLLSHESRMDKDVAQTNVKDSTLPSVNLV 209
           +   SVQ+  SLLL+ ES+ +  +    + ++ LPSVN+V
Sbjct: 205 TDSPSVQEVMSLLLTQESQNESKL----ISETALPSVNIV 240

BLAST of Moc11g25770 vs. TAIR 10
Match: AT1G34070.1 (CONTAINS InterPro DOMAIN/s: Retrotransposon gag protein (InterPro:IPR005162); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G48050.1); Has 648 Blast hits to 647 proteins in 29 species: Archae - 0; Bacteria - 0; Metazoa - 16; Fungi - 25; Plants - 607; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 43.9 bits (102), Expect = 1.8e-04
Identity = 24/77 (31.17%), Postives = 43/77 (55.84%), Query Frame = 0

Query: 116 GNSTIKDYVRQVKSLVDSLLATGQLVTPEDHVLYILSGLGLEYESTISVITTQSGEVSVQ 175
           G+  + DY R++K L DSL      VT  + V+Y+L+GL  ++++ I+VI  +    S  
Sbjct: 127 GDMRVADYYRKMKKLADSLRNVDVPVTDRNLVMYVLNGLNPKFDNIINVIKHRQPFPSFD 186

Query: 176 KATSLLLSHESRMDKDV 193
            A ++L   E R+ + +
Sbjct: 187 DAATMLQEEEDRLKRAI 203

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022158089.11.5e-1948.65uncharacterized protein LOC111024658 [Momordica charantia][more]
XP_022136882.11.0e-1753.47dr1-associated corepressor homolog isoform X1 [Momordica charantia][more]
XP_022136883.11.0e-1753.47dr1-associated corepressor homolog isoform X2 [Momordica charantia][more]
XP_022154487.13.5e-1344.44uncharacterized protein LOC111021757 [Momordica charantia][more]
KAA0048297.11.9e-1142.00Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. m... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DYD57.1e-2048.65uncharacterized protein LOC111024658 OS=Momordica charantia OX=3673 GN=LOC111024... [more]
A0A6J1C8R25.1e-1853.47dr1-associated corepressor homolog isoform X2 OS=Momordica charantia OX=3673 GN=... [more]
A0A6J1C6N95.1e-1853.47dr1-associated corepressor homolog isoform X1 OS=Momordica charantia OX=3673 GN=... [more]
A0A6J1DLT91.7e-1344.44uncharacterized protein LOC111021757 OS=Momordica charantia OX=3673 GN=LOC111021... [more]
A0A5A7U2339.2e-1242.00Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cucumis melo var.... [more]
Match NameE-valueIdentityDescription
AT1G34070.11.8e-0431.17CONTAINS InterPro DOMAIN/s: Retrotransposon gag protein (InterPro:IPR005162); BE... [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF14223Retrotran_gag_2coord: 105..190
e-value: 7.1E-10
score: 38.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..27
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 54..85
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 62..85
NoneNo IPR availablePANTHERPTHR34222:SF48SUBFAMILY NOT NAMEDcoord: 111..200
NoneNo IPR availablePANTHERPTHR34222FAMILY NOT NAMEDcoord: 111..200

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc11g25770.1Moc11g25770.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008152 metabolic process
molecular_function GO:0005488 binding
molecular_function GO:0016740 transferase activity