Moc11g11420 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc11g11420
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Cytochrome P450, putative
Location	chr11: 8342152 .. 8343161 (-)
RNA-Seq Expression	Moc11g11420
Synteny	Moc11g11420

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTGCTGGATATGATACTTCATCTGTTCTTATTACATTCATGGTGCGGCTTTTGGCTAATAATCCAACCATTCATGCAGCTGTTCTTCAAGGTATGCTTTTAAGTTCTAACTCTCTTAAATTTTCAATTTTACTCTGAGCTCATTATCATGTTAGCCTTAAAATGCAGAGAAAGTAATTTTATGTTTAGTAGAAACCCAAAGAAATACTGTCTGGAAATGGATAAAAGAAACAAACCGAGATCAAGAAATTGGTATGCCAAAATGAAAAACAGAAACTCGATAACTCTAGATAGATAATAAGAGACTCATGCATTGCTATATAAAAACCTGAAAGGACTTCAACTTCTAACTCGAATCATATAGACTCGAGAAGACGAGCGAAAGTTAACATTATCGGGTTGCTTGTGGTCTGTGGTCACAGAACAGGAAGAGATCGCAGGAAGTAAGAAATGTGGAGAGTTGCTCACTTGGGAAGATCTTGCCAAGATGAAGTACACATGGAGTGTTGCATTAGAGACCTTGAGACTAGTGGCACCTATCTTTGGAGGATTCAGAAGGGCTCTGAAAGATATTGAATTTGGTGGCTACCTTATTCCCAAAGGATGGCAAGTAAGCAGAGTTTTCTTTCTTTCTTCTTTTGGTTGAACAAACATGTGAAAATATTCATCCAACAATAATTCAAATGGATTTTCAGATATTCTGGGCAGCACCAATGACTCAACTGGATGGTACAATATTCGAAGAACCACTTAAGTTCGACCCGAGTAGATTCGAGAATCAGGCATCGATTCCACCGTATTGCTTCGTTGCATTTGGCGGGGGTCCAAGAATTTGTCCTGGATACGAGTTTGGAAGGGTTGAAACTCTTGTTACCATCCATTACTTGATCACTCAGTTCACATGGAAGCTACTTTCAGATGAGCTTTTTATCAGAGATCCAATGCCAATTCCTACCAAAGAATTGCCTATCAAAATTTTTGCTACAGAAACTTCCATTTCATTGTAG

mRNA sequence

ATGGTTGCTGGATATGATACTTCATCTGTTCTTATTACATTCATGGTGCGGCTTTTGGCTAATAATCCAACCATTCATGCAGCTGTTCTTCAAGAACAGGAAGAGATCGCAGGAAGTAAGAAATGTGGAGAGTTGCTCACTTGGGAAGATCTTGCCAAGATGAAGTACACATGGAGTGTTGCATTAGAGACCTTGAGACTAGTGGCACCTATCTTTGGAGGATTCAGAAGGGCTCTGAAAGATATTGAATTTGGTGGCTACCTTATTCCCAAAGGATGGCAAATATTCTGGGCAGCACCAATGACTCAACTGGATGGTACAATATTCGAAGAACCACTTAAGTTCGACCCGAGTAGATTCGAGAATCAGGCATCGATTCCACCGTATTGCTTCGTTGCATTTGGCGGGGGTCCAAGAATTTGTCCTGGATACGAGTTTGGAAGGGTTGAAACTCTTGTTACCATCCATTACTTGATCACTCAGTTCACATGGAAGCTACTTTCAGATGAGCTTTTTATCAGAGATCCAATGCCAATTCCTACCAAAGAATTGCCTATCAAAATTTTTGCTACAGAAACTTCCATTTCATTGTAG

Coding sequence (CDS)

ATGGTTGCTGGATATGATACTTCATCTGTTCTTATTACATTCATGGTGCGGCTTTTGGCTAATAATCCAACCATTCATGCAGCTGTTCTTCAAGAACAGGAAGAGATCGCAGGAAGTAAGAAATGTGGAGAGTTGCTCACTTGGGAAGATCTTGCCAAGATGAAGTACACATGGAGTGTTGCATTAGAGACCTTGAGACTAGTGGCACCTATCTTTGGAGGATTCAGAAGGGCTCTGAAAGATATTGAATTTGGTGGCTACCTTATTCCCAAAGGATGGCAAATATTCTGGGCAGCACCAATGACTCAACTGGATGGTACAATATTCGAAGAACCACTTAAGTTCGACCCGAGTAGATTCGAGAATCAGGCATCGATTCCACCGTATTGCTTCGTTGCATTTGGCGGGGGTCCAAGAATTTGTCCTGGATACGAGTTTGGAAGGGTTGAAACTCTTGTTACCATCCATTACTTGATCACTCAGTTCACATGGAAGCTACTTTCAGATGAGCTTTTTATCAGAGATCCAATGCCAATTCCTACCAAAGAATTGCCTATCAAAATTTTTGCTACAGAAACTTCCATTTCATTGTAG

Protein sequence

MVAGYDTSSVLITFMVRLLANNPTIHAAVLQEQEEIAGSKKCGELLTWEDLAKMKYTWSVALETLRLVAPIFGGFRRALKDIEFGGYLIPKGWQIFWAAPMTQLDGTIFEEPLKFDPSRFENQASIPPYCFVAFGGGPRICPGYEFGRVETLVTIHYLITQFTWKLLSDELFIRDPMPIPTKELPIKIFATETSISL

Homology

[Close]

BLAST of Moc11g11420 vs. NCBI nr
Match: XP_022139355.1 (LOW QUALITY PROTEIN: cytochrome P450 716B1-like [Momordica charantia])

HSP 1 Score: 366.7 bits (940), Expect = 1.3e-97
Identity = 171/197 (86.80%), Postives = 185/197 (93.91%), Query Frame = 0


Query: 1   MVAGYDTSSVLITFMVRLLANNPTIHAAVLQEQEEIAGSKKCGELLTWEDLAKMKYTWSV 60
           MVAGYDTSSVLITFMVR LANNPT++AAVLQEQEEIA SK+CGELLTWEDLAKMKYTW V
Sbjct: 282 MVAGYDTSSVLITFMVRFLANNPTVYAAVLQEQEEIARSKECGELLTWEDLAKMKYTWRV 341

Query: 61  ALETLRLVAPIFGGFRRALKDIEFGGYLIPKGWQIFWAAPMTQLDGTIFEEPLKFDPSRF 120
           ALETLRLVAP+FGGFR+A+KDIEFGGYLIPKGWQIFWA+PMT LD TIFEEPLKFDP+RF
Sbjct: 342 ALETLRLVAPVFGGFRKAMKDIEFGGYLIPKGWQIFWASPMTHLDDTIFEEPLKFDPNRF 401

Query: 121 ENQASIPPYCFVAFGGGPRICPGYEFGRVETLVTIHYLITQFTWKLLSDELFIRDPMPIP 180
           ENQAS+P YCFVAFGGGPRICPGYEF RVETLVTIHYLITQFTWKLL D+LFIRDPMP+P
Sbjct: 402 ENQASVPXYCFVAFGGGPRICPGYEFARVETLVTIHYLITQFTWKLLLDDLFIRDPMPVP 461

Query: 181 TKELPIKIFATETSISL 198
           TK LPIK+   ETS++L
Sbjct: 462 TKGLPIKVCPRETSMTL 478

[Close]

BLAST of Moc11g11420 vs. NCBI nr
Match: XP_038899130.1 (LOW QUALITY PROTEIN: cytochrome P450 716B1-like [Benincasa hispida])

HSP 1 Score: 340.5 bits (872), Expect = 9.7e-90
Identity = 162/204 (79.41%), Postives = 178/204 (87.25%), Query Frame = 0


Query: 1   MVAGYDTSSVLITFMVRLLANNPTIHAAVLQEQEEIAGSKKCGELLTWEDLAKMKYTWSV 60
           MVAG+DTSSVLITF++R LANNPT++ AVLQEQEEIA SKKCGELLTWEDLAKMKYTW V
Sbjct: 280 MVAGFDTSSVLITFIMRFLANNPTVYVAVLQEQEEIARSKKCGELLTWEDLAKMKYTWRV 339

Query: 61  ALETLRLVAPIFGGFRRALKDIEFGGYLIPKGWQIFWAAPMTQLDGTIFEEPLKFDPSRF 120
           ALETLRLVAPIFGGFR+A+ DIEFGGYLIPKGWQIFWA+P+T LDGTIF EPLKFDP+RF
Sbjct: 340 ALETLRLVAPIFGGFRKAMNDIEFGGYLIPKGWQIFWASPVTHLDGTIFREPLKFDPNRF 399

Query: 121 ENQASIPPYCFVAFG-------GGPRICPGYEFGRVETLVTIHYLITQFTWKLLSDELFI 180
           +N  SIPPYCFV FG       GGPRICPG EF RVETLVTIHYLIT+FTWKLL D+ FI
Sbjct: 400 DNPTSIPPYCFVGFGGGPRICPGGPRICPGNEFARVETLVTIHYLITRFTWKLLLDDHFI 459

Query: 181 RDPMPIPTKELPIKIFATETSISL 198
           RDPMP PTK +PIKI   ETS++L
Sbjct: 460 RDPMPTPTKGMPIKICPRETSMAL 483

[Close]

BLAST of Moc11g11420 vs. NCBI nr
Match: XP_008457111.1 (PREDICTED: cytochrome P450 716B1-like [Cucumis melo] >KAA0047356.1 cytochrome P450 716B1-like protein [Cucumis melo var. makuwa] >TYK14034.1 cytochrome P450 716B1-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 334.7 bits (857), Expect = 5.3e-88
Identity = 155/193 (80.31%), Postives = 171/193 (88.60%), Query Frame = 0


Query: 1   MVAGYDTSSVLITFMVRLLANNPTIHAAVLQEQEEIAGSKKCGELLTWEDLAKMKYTWSV 60
           MVAG+DTSSVLITFM+R LANNPT++AAVLQEQEEIA SKKCGELL WEDLAKMKYTW V
Sbjct: 281 MVAGFDTSSVLITFMMRSLANNPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRV 340

Query: 61  ALETLRLVAPIFGGFRRALKDIEFGGYLIPKGWQIFWAAPMTQLDGTIFEEPLKFDPSRF 120
           ALETLRLVAPIFGGFR+A+ DI+FGGYLIPKGWQIFW +P+T LD  IF EP KFDP RF
Sbjct: 341 ALETLRLVAPIFGGFRKAMNDIQFGGYLIPKGWQIFWTSPVTHLDDNIFREPSKFDPDRF 400

Query: 121 ENQASIPPYCFVAFGGGPRICPGYEFGRVETLVTIHYLITQFTWKLLSDELFIRDPMPIP 180
           +N ASIPPYCF+ FG GPRICPG EF RVETLVTIHYLITQFTWKL+SD+ FIRDPMP P
Sbjct: 401 KNPASIPPYCFLGFGSGPRICPGNEFARVETLVTIHYLITQFTWKLISDDHFIRDPMPTP 460

Query: 181 TKELPIKIFATET 194
           TK +PIKI+  +T
Sbjct: 461 TKGMPIKIWPRQT 473

[Close]

BLAST of Moc11g11420 vs. NCBI nr
Match: XP_004140824.1 (cytochrome P450 716B1 [Cucumis sativus] >KGN57442.1 hypothetical protein Csa_010272 [Cucumis sativus])

HSP 1 Score: 332.0 bits (850), Expect = 3.4e-87
Identity = 155/194 (79.90%), Postives = 170/194 (87.63%), Query Frame = 0


Query: 1   MVAGYDTSSVLITFMVRLLANNPTIHAAVLQEQEEIAGSKKCGELLTWEDLAKMKYTWSV 60
           +VAG+DTSSVLITFM+R LANNPT++AAVLQEQEEIA SKKCGELL WEDLAKMKYTW V
Sbjct: 281 LVAGFDTSSVLITFMMRNLANNPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRV 340

Query: 61  ALETLRLVAPIFGGFRRALKDIEFGGYLIPKGWQIFWAAPMTQLDGTIFEEPLKFDPSRF 120
           ALETLRLVAPIFGGFR+A+ DI+FGGYLIPKGWQIFW +P+T LD TIF EP KFDP RF
Sbjct: 341 ALETLRLVAPIFGGFRKAMNDIQFGGYLIPKGWQIFWTSPVTHLDDTIFREPSKFDPDRF 400

Query: 121 ENQASIPPYCFVAFGGGPRICPGYEFGRVETLVTIHYLITQFTWKLLSDELFIRDPMPIP 180
           +N ASIPPYCF+ FG GPRICPG EF RVETLVTIHYLITQFTWKLL D+ FIRDPMP P
Sbjct: 401 KNPASIPPYCFLGFGSGPRICPGNEFARVETLVTIHYLITQFTWKLLLDDHFIRDPMPTP 460

Query: 181 TKELPIKIFATETS 195
           TK +PIKI+    S
Sbjct: 461 TKGMPIKIWPRSQS 474

[Close]

BLAST of Moc11g11420 vs. NCBI nr
Match: KAA8523694.1 (hypothetical protein F0562_010117 [Nyssa sinensis])

HSP 1 Score: 318.2 bits (814), Expect = 5.1e-83
Identity = 143/189 (75.66%), Postives = 170/189 (89.95%), Query Frame = 0


Query: 1   MVAGYDTSSVLITFMVRLLANNPTIHAAVLQEQEEIAGSKKCGELLTWEDLAKMKYTWSV 60
           MVAG+DTSSVLI+F++RLL+N+PT++AAVLQEQEEIA SK CGELLTWEDLAKMKYTW  
Sbjct: 295 MVAGHDTSSVLISFIMRLLSNDPTVYAAVLQEQEEIAKSKPCGELLTWEDLAKMKYTWRA 354

Query: 61  ALETLRLVAPIFGGFRRALKDIEFGGYLIPKGWQIFWAAPMTQLDGTIFEEPLKFDPSRF 120
           A+ETLR+  PIFGGFR+ LKDIE+ GYLIPKGWQIFWA+ MT +D +IF++P KFDP+RF
Sbjct: 355 AMETLRMFPPIFGGFRKTLKDIEYKGYLIPKGWQIFWASSMTHMDSSIFQDPSKFDPTRF 414

Query: 121 ENQASIPPYCFVAFGGGPRICPGYEFGRVETLVTIHYLITQFTWKL-LSDELFIRDPMPI 180
           ENQAS+PPYCFVAFGGGPRICPGYEF R+ETLVT+HYL+T+FTWKL  +D  F RDPMP+
Sbjct: 415 ENQASVPPYCFVAFGGGPRICPGYEFARIETLVTVHYLVTRFTWKLCCTDNFFSRDPMPV 474

Query: 181 PTKELPIKI 189
           PT+ LPI+I
Sbjct: 475 PTEGLPIEI 483

[Close]

BLAST of Moc11g11420 vs. ExPASy Swiss-Prot
Match: A0A0S2II38 (Beta-amyrin 28-monooxygenase OS=Kalopanax septemlobus OX=228393 GN=CYP716A94 PE=1 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 3.8e-57
Identity = 101/190 (53.16%), Postives = 136/190 (71.58%), Query Frame = 0


Query: 1   MVAGYDTSSVLITFMVRLLANNPTIHAAVLQEQEEIAGSKKCGELLTWEDLAKMKYTWSV 60
           ++ G+DT+S   TF+V+ LA  P I+  V +EQ EIA SKK GELL WED+ KMKY+W+V
Sbjct: 289 LIGGHDTASSACTFVVKFLAELPEIYEGVYKEQMEIAKSKKAGELLNWEDIQKMKYSWNV 348

Query: 61  ALETLRLVAPIFGGFRRALKDIEFGGYLIPKGWQIFWAAPMTQLDGTIFEEPLKFDPSRF 120
           A E LRL  P+ G FR AL D  + G+ IPKGW+++W+A  T  +  +F EPLKFDPSRF
Sbjct: 349 ACEVLRLAPPLQGAFREALTDFSYNGFSIPKGWKLYWSANSTHRNSEVFPEPLKFDPSRF 408

Query: 121 ENQASIPPYCFVAFGGGPRICPGYEFGRVETLVTIHYLITQFTW-KLLSDELFIRDPMPI 180
           E  A  PPY FV FGGGPR+CPG E+ R+E LV +H+++ +F W K++ DE  + +PMPI
Sbjct: 409 EG-AGPPPYSFVPFGGGPRMCPGKEYARLEILVFMHHVVKRFKWEKVIPDEKIVVNPMPI 468

Query: 181 PTKELPIKIF 190
           P K LP+++F
Sbjct: 469 PAKGLPVRLF 477

[Close]

BLAST of Moc11g11420 vs. ExPASy Swiss-Prot
Match: I7C6E8 (Beta-amyrin 28-monooxygenase OS=Panax ginseng OX=4054 GN=CYP716A52v2 PE=1 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 1.9e-56
Identity = 99/190 (52.11%), Postives = 135/190 (71.05%), Query Frame = 0


Query: 1   MVAGYDTSSVLITFMVRLLANNPTIHAAVLQEQEEIAGSKKCGELLTWEDLAKMKYTWSV 60
           ++ G+DT+S   TF+V+ LA  P I+  V QEQ EI  SKK GELL WED+ KMKY+W+V
Sbjct: 289 LIGGHDTASSACTFVVKFLAELPQIYEGVYQEQMEIVKSKKAGELLKWEDIQKMKYSWNV 348

Query: 61  ALETLRLVAPIFGGFRRALKDIEFGGYLIPKGWQIFWAAPMTQLDGTIFEEPLKFDPSRF 120
           A E LRL  P+ G FR AL D  + G+ IPKGW+++W+A  T ++  +F EPLKFDPSRF
Sbjct: 349 ACEVLRLAPPLQGAFREALSDFTYNGFSIPKGWKLYWSANSTHINSEVFPEPLKFDPSRF 408

Query: 121 ENQASIPPYCFVAFGGGPRICPGYEFGRVETLVTIHYLITQFTW-KLLSDELFIRDPMPI 180
           +  A  PP+ FV FGGGPR+CPG E+ R+E LV +H+L+ +F W K++ DE  + +PMPI
Sbjct: 409 DG-AGPPPFSFVPFGGGPRMCPGKEYARLEILVFMHHLVKRFKWEKVIPDEKIVVNPMPI 468

Query: 181 PTKELPIKIF 190
           P   LP+++F
Sbjct: 469 PANGLPVRLF 477

[Close]

BLAST of Moc11g11420 vs. ExPASy Swiss-Prot
Match: K4CEE8 (Beta-amyrin 28-monooxygenase OS=Solanum lycopersicum OX=4081 GN=CYP716A46 PE=1 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 6.8e-54
Identity = 98/190 (51.58%), Postives = 134/190 (70.53%), Query Frame = 0


Query: 1   MVAGYDTSSVLITFMVRLLANNPTIHAAVLQEQEEIAGSKKCGELLTWEDLAKMKYTWSV 60
           ++ G+DT+S    F+V+ LA  P I+  V +EQ EIA SK  GELL+WED+ KMKY+W+V
Sbjct: 288 LIGGHDTASSACAFIVKYLAELPEIYDQVYKEQIEIAKSKGPGELLSWEDIKKMKYSWNV 347

Query: 61  ALETLRLVAPIFGGFRRALKDIEFGGYLIPKGWQIFWAAPMTQLDGTIFEEPLKFDPSRF 120
           A E LRL  P+ G FR AL D  F G+ IPKGW+I+W+A  T ++  +F +PLKFDPSRF
Sbjct: 348 ACEVLRLAPPLQGAFREALVDFTFNGFSIPKGWKIYWSANSTHINPEVFVDPLKFDPSRF 407

Query: 121 ENQASIPPYCFVAFGGGPRICPGYEFGRVETLVTIHYLITQFTW-KLLSDELFIRDPMPI 180
           E      PY FV FGGGPR+CPG E+ R+E LV +H+L+ +F+W K++ DE  + +PMPI
Sbjct: 408 EGNGP-APYTFVPFGGGPRMCPGKEYARLEILVFMHHLVKRFSWKKIIRDEKIVVNPMPI 467

Query: 181 PTKELPIKIF 190
           P   LPI+++
Sbjct: 468 PANGLPIRLY 476

[Close]

BLAST of Moc11g11420 vs. ExPASy Swiss-Prot
Match: Q2MJ20 (Beta-amyrin 28-monooxygenase OS=Medicago truncatula OX=3880 GN=CYP716A12 PE=1 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 2.0e-53
Identity = 98/190 (51.58%), Postives = 130/190 (68.42%), Query Frame = 0


Query: 1   MVAGYDTSSVLITFMVRLLANNPTIHAAVLQEQEEIAGSKKCGELLTWEDLAKMKYTWSV 60
           ++ G+DT+SV  TF+V+ L   P I+  V QEQ EIA SK  GELL W+DL KMKY+W+V
Sbjct: 287 LIGGHDTASVACTFLVKYLGELPHIYDKVYQEQMEIAKSKPAGELLNWDDLKKMKYSWNV 346

Query: 61  ALETLRLVAPIFGGFRRALKDIEFGGYLIPKGWQIFWAAPMTQLDGTIFEEPLKFDPSRF 120
           A E +RL  P+ GGFR A+ D  F G+ IPKGW+++W+A  T  +   F  P KFDP+RF
Sbjct: 347 ACEVMRLSPPLQGGFREAITDFMFNGFSIPKGWKLYWSANSTHKNAECFPMPEKFDPTRF 406

Query: 121 ENQASIPPYCFVAFGGGPRICPGYEFGRVETLVTIHYLITQFTW-KLLSDELFIRDPMPI 180
           E      PY FV FGGGPR+CPG E+ R+E LV +H L+ +F W K++ DE  I DP PI
Sbjct: 407 EGNGP-APYTFVPFGGGPRMCPGKEYARLEILVFMHNLVKRFKWEKVIPDEKIIVDPFPI 466

Query: 181 PTKELPIKIF 190
           P K+LPI+++
Sbjct: 467 PAKDLPIRLY 475

[Close]

BLAST of Moc11g11420 vs. ExPASy Swiss-Prot
Match: A5BFI4 (Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A17 PE=1 SV=2)

HSP 1 Score: 204.9 bits (520), Expect = 8.3e-52
Identity = 95/189 (50.26%), Postives = 130/189 (68.78%), Query Frame = 0


Query: 1   MVAGYDTSSVLITFMVRLLANNPTIHAAVLQEQEEIAGSKKCGELLTWEDLAKMKYTWSV 60
           ++ G+DT+S  ITF+V+ +A  P I+  V +EQ EIA SK  GELL W+D+ KM+Y+W+V
Sbjct: 288 LIGGHDTASSAITFLVKYMAELPHIYEKVYKEQMEIANSKAPGELLNWDDVQKMRYSWNV 347

Query: 61  ALETLRLVAPIFGGFRRALKDIEFGGYLIPKGWQIFWAAPMTQLDGTIFEEPLKFDPSRF 120
           A E +RL  P+ G FR A+ D  F G+ IPKGW+++W+A  T      F +P KFDP+RF
Sbjct: 348 ACEVMRLAPPLQGAFREAITDFVFNGFSIPKGWKLYWSANSTHKSLECFPQPEKFDPTRF 407

Query: 121 ENQASIPPYCFVAFGGGPRICPGYEFGRVETLVTIHYLITQFTW-KLLSDELFIRDPMPI 180
           E  A   PY FV FGGGPR+CPG E+ R+E L+ +H L+ +F W KLL DE  I DPMP+
Sbjct: 408 EG-AGPAPYTFVPFGGGPRMCPGKEYARLEILIFMHNLVKRFKWDKLLPDEKIIVDPMPM 467

Query: 181 PTKELPIKI 189
           P K LP+++
Sbjct: 468 PAKGLPVRL 475

[Close]

BLAST of Moc11g11420 vs. ExPASy TrEMBL
Match: A0A6J1CC35 (LOW QUALITY PROTEIN: cytochrome P450 716B1-like OS=Momordica charantia OX=3673 GN=LOC111010306 PE=3 SV=1)

HSP 1 Score: 366.7 bits (940), Expect = 6.1e-98
Identity = 171/197 (86.80%), Postives = 185/197 (93.91%), Query Frame = 0


Query: 1   MVAGYDTSSVLITFMVRLLANNPTIHAAVLQEQEEIAGSKKCGELLTWEDLAKMKYTWSV 60
           MVAGYDTSSVLITFMVR LANNPT++AAVLQEQEEIA SK+CGELLTWEDLAKMKYTW V
Sbjct: 282 MVAGYDTSSVLITFMVRFLANNPTVYAAVLQEQEEIARSKECGELLTWEDLAKMKYTWRV 341

Query: 61  ALETLRLVAPIFGGFRRALKDIEFGGYLIPKGWQIFWAAPMTQLDGTIFEEPLKFDPSRF 120
           ALETLRLVAP+FGGFR+A+KDIEFGGYLIPKGWQIFWA+PMT LD TIFEEPLKFDP+RF
Sbjct: 342 ALETLRLVAPVFGGFRKAMKDIEFGGYLIPKGWQIFWASPMTHLDDTIFEEPLKFDPNRF 401

Query: 121 ENQASIPPYCFVAFGGGPRICPGYEFGRVETLVTIHYLITQFTWKLLSDELFIRDPMPIP 180
           ENQAS+P YCFVAFGGGPRICPGYEF RVETLVTIHYLITQFTWKLL D+LFIRDPMP+P
Sbjct: 402 ENQASVPXYCFVAFGGGPRICPGYEFARVETLVTIHYLITQFTWKLLLDDLFIRDPMPVP 461

Query: 181 TKELPIKIFATETSISL 198
           TK LPIK+   ETS++L
Sbjct: 462 TKGLPIKVCPRETSMTL 478

[Close]

BLAST of Moc11g11420 vs. ExPASy TrEMBL
Match: A0A5A7TZG7 (Cytochrome P450 716B1-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold363G00020 PE=3 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 2.6e-88
Identity = 155/193 (80.31%), Postives = 171/193 (88.60%), Query Frame = 0


Query: 1   MVAGYDTSSVLITFMVRLLANNPTIHAAVLQEQEEIAGSKKCGELLTWEDLAKMKYTWSV 60
           MVAG+DTSSVLITFM+R LANNPT++AAVLQEQEEIA SKKCGELL WEDLAKMKYTW V
Sbjct: 281 MVAGFDTSSVLITFMMRSLANNPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRV 340

Query: 61  ALETLRLVAPIFGGFRRALKDIEFGGYLIPKGWQIFWAAPMTQLDGTIFEEPLKFDPSRF 120
           ALETLRLVAPIFGGFR+A+ DI+FGGYLIPKGWQIFW +P+T LD  IF EP KFDP RF
Sbjct: 341 ALETLRLVAPIFGGFRKAMNDIQFGGYLIPKGWQIFWTSPVTHLDDNIFREPSKFDPDRF 400

Query: 121 ENQASIPPYCFVAFGGGPRICPGYEFGRVETLVTIHYLITQFTWKLLSDELFIRDPMPIP 180
           +N ASIPPYCF+ FG GPRICPG EF RVETLVTIHYLITQFTWKL+SD+ FIRDPMP P
Sbjct: 401 KNPASIPPYCFLGFGSGPRICPGNEFARVETLVTIHYLITQFTWKLISDDHFIRDPMPTP 460

Query: 181 TKELPIKIFATET 194
           TK +PIKI+  +T
Sbjct: 461 TKGMPIKIWPRQT 473

[Close]

BLAST of Moc11g11420 vs. ExPASy TrEMBL
Match: A0A1S3C4V0 (cytochrome P450 716B1-like OS=Cucumis melo OX=3656 GN=LOC103496862 PE=3 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 2.6e-88
Identity = 155/193 (80.31%), Postives = 171/193 (88.60%), Query Frame = 0


Query: 1   MVAGYDTSSVLITFMVRLLANNPTIHAAVLQEQEEIAGSKKCGELLTWEDLAKMKYTWSV 60
           MVAG+DTSSVLITFM+R LANNPT++AAVLQEQEEIA SKKCGELL WEDLAKMKYTW V
Sbjct: 281 MVAGFDTSSVLITFMMRSLANNPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRV 340

Query: 61  ALETLRLVAPIFGGFRRALKDIEFGGYLIPKGWQIFWAAPMTQLDGTIFEEPLKFDPSRF 120
           ALETLRLVAPIFGGFR+A+ DI+FGGYLIPKGWQIFW +P+T LD  IF EP KFDP RF
Sbjct: 341 ALETLRLVAPIFGGFRKAMNDIQFGGYLIPKGWQIFWTSPVTHLDDNIFREPSKFDPDRF 400

Query: 121 ENQASIPPYCFVAFGGGPRICPGYEFGRVETLVTIHYLITQFTWKLLSDELFIRDPMPIP 180
           +N ASIPPYCF+ FG GPRICPG EF RVETLVTIHYLITQFTWKL+SD+ FIRDPMP P
Sbjct: 401 KNPASIPPYCFLGFGSGPRICPGNEFARVETLVTIHYLITQFTWKLISDDHFIRDPMPTP 460

Query: 181 TKELPIKIFATET 194
           TK +PIKI+  +T
Sbjct: 461 TKGMPIKIWPRQT 473

[Close]

BLAST of Moc11g11420 vs. ExPASy TrEMBL
Match: A0A0A0LA02 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G186690 PE=3 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 1.7e-87
Identity = 155/194 (79.90%), Postives = 170/194 (87.63%), Query Frame = 0


Query: 1   MVAGYDTSSVLITFMVRLLANNPTIHAAVLQEQEEIAGSKKCGELLTWEDLAKMKYTWSV 60
           +VAG+DTSSVLITFM+R LANNPT++AAVLQEQEEIA SKKCGELL WEDLAKMKYTW V
Sbjct: 281 LVAGFDTSSVLITFMMRNLANNPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRV 340

Query: 61  ALETLRLVAPIFGGFRRALKDIEFGGYLIPKGWQIFWAAPMTQLDGTIFEEPLKFDPSRF 120
           ALETLRLVAPIFGGFR+A+ DI+FGGYLIPKGWQIFW +P+T LD TIF EP KFDP RF
Sbjct: 341 ALETLRLVAPIFGGFRKAMNDIQFGGYLIPKGWQIFWTSPVTHLDDTIFREPSKFDPDRF 400

Query: 121 ENQASIPPYCFVAFGGGPRICPGYEFGRVETLVTIHYLITQFTWKLLSDELFIRDPMPIP 180
           +N ASIPPYCF+ FG GPRICPG EF RVETLVTIHYLITQFTWKLL D+ FIRDPMP P
Sbjct: 401 KNPASIPPYCFLGFGSGPRICPGNEFARVETLVTIHYLITQFTWKLLLDDHFIRDPMPTP 460

Query: 181 TKELPIKIFATETS 195
           TK +PIKI+    S
Sbjct: 461 TKGMPIKIWPRSQS 474

[Close]

BLAST of Moc11g11420 vs. ExPASy TrEMBL
Match: A0A2N9F2Q7 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS9063 PE=3 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 5.9e-85
Identity = 146/193 (75.65%), Postives = 169/193 (87.56%), Query Frame = 0


Query: 1   MVAGYDTSSVLITFMVRLLANNPTIHAAVLQEQEEIAGSKKCGELLTWEDLAKMKYTWSV 60
           MVAG+DTSSVLITF++RLLAN PTI+AAVLQEQEEI  SK  GE LTWEDLAKMKYTW V
Sbjct: 199 MVAGHDTSSVLITFIMRLLANEPTIYAAVLQEQEEIVKSKPSGEFLTWEDLAKMKYTWRV 258

Query: 61  ALETLRLVAPIFGGFRRALKDIEFGGYLIPKGWQIFWAAPMTQLDGTIFEEPLKFDPSRF 120
           ++ETLR++ P+FGGFR+ALKDIE+GGYLIPKGWQ+FW +PMT +D +IF EP KFDPSRF
Sbjct: 259 SMETLRMLPPVFGGFRKALKDIEYGGYLIPKGWQVFWVSPMTHMDSSIFPEPSKFDPSRF 318

Query: 121 ENQASIPPYCFVAFGGGPRICPGYEFGRVETLVTIHYLITQFTWKLLSDELFIRDPMPIP 180
           ENQAS+PPYCFVAF GGPRICPGYEF R+ETLVTIHYLIT FTWKL +D  F RDPMP+P
Sbjct: 319 ENQASVPPYCFVAFRGGPRICPGYEFARIETLVTIHYLITHFTWKLCADNSFSRDPMPVP 378

Query: 181 TKELPIKIFATET 194
           T+ LPI++   +T
Sbjct: 379 TQGLPIQLVPKKT 391

[Close]

BLAST of Moc11g11420 vs. TAIR 10
Match: AT5G36110.1 (cytochrome P450, family 716, subfamily A, polypeptide 1 )

HSP 1 Score: 194.9 bits (494), Expect = 6.1e-50
Identity = 87/191 (45.55%), Postives = 130/191 (68.06%), Query Frame = 0


Query: 1   MVAGYDTSSVLITFMVRLLANNPTIHAAVLQEQEEIAGSKKCGELLTWEDLAKMKYTWSV 60
           ++ G+DT+S++ TF+V  LA  P ++  VLQEQ+EI   KK  E L WED+ KM+Y+W+V
Sbjct: 285 LIGGHDTASIVCTFVVNYLAEFPHVYQRVLQEQKEILKEKKEKEGLRWEDIEKMRYSWNV 344

Query: 61  ALETLRLVAPIFGGFRRALKDIEFGGYLIPKGWQIFWAAPMTQLDGTIFEEPLKFDPSRF 120
           A E +R+V P+ G FR A+    F G+ IPKGW+++W+A  T ++   F EP +F+P+RF
Sbjct: 345 ACEVMRIVPPLSGTFREAIDHFSFKGFYIPKGWKLYWSATATHMNPDYFPEPERFEPNRF 404

Query: 121 ENQASIPPYCFVAFGGGPRICPGYEFGRVETLVTIHYLITQFTWKLL--SDELFIRDPMP 180
           E      PY +V FGGGPR+CPG E+ R+E L+ +H L+ +F W+ +  ++   + DP+P
Sbjct: 405 EGSGP-KPYTYVPFGGGPRMCPGKEYARLEILIFMHNLVNRFKWEKVFPNENKIVVDPLP 464

Query: 181 IPTKELPIKIF 190
           IP K LPI+IF
Sbjct: 465 IPDKGLPIRIF 474

[Close]

BLAST of Moc11g11420 vs. TAIR 10
Match: AT2G42850.1 (cytochrome P450, family 718 )

HSP 1 Score: 185.7 bits (470), Expect = 3.7e-47
Identity = 84/187 (44.92%), Postives = 123/187 (65.78%), Query Frame = 0


Query: 3   AGYDTSSVLITFMVRLLANNPTIHAAVLQEQEEIAGSKKCGELLTWEDLAKMKYTWSVAL 62
           A +DT+S  ++   ++LA +PT    +LQE  +I  +K  GE LT ED+ KMKY+W V  
Sbjct: 296 AAHDTTSYAMSMTFKMLAQHPTCRDTLLQEHAQIKANKGEGEYLTVEDVKKMKYSWQVVR 355

Query: 63  ETLRLVAPIFGGFRRALKDIEFGGYLIPKGWQIFWAAPMTQLDGTIFEEPLKFDPSRFEN 122
           ET+RL  PIFG FR+A+ DI++GGY IPKGW+I W    T  +  IF++P+ FDP+RF+ 
Sbjct: 356 ETMRLSPPIFGSFRKAVADIDYGGYTIPKGWKILWTTYGTHYNPEIFQDPMSFDPTRFDK 415

Query: 123 QASIPPYCFVAFGGGPRICPGYEFGRVETLVTIHYLITQFTWKLL-SDELFIRDPMPIPT 182
              I  Y ++ FGGGPR+C G++  ++  LV +H+++T F W L+  DE    DP+P P+
Sbjct: 416 --PIQAYTYLPFGGGPRLCAGHQLAKISILVFMHFVVTGFDWSLVYPDETISMDPLPFPS 475

Query: 183 KELPIKI 189
             +PIKI
Sbjct: 476 LGMPIKI 480

[Close]

BLAST of Moc11g11420 vs. TAIR 10
Match: AT5G45340.1 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 146.0 bits (367), Expect = 3.3e-35
Identity = 77/187 (41.18%), Postives = 110/187 (58.82%), Query Frame = 0


Query: 3   AGYDTSSVLITFMVRLLANNPTIHAAVLQEQEEIAGSKKCGELLTWEDLAKMKYTWSVAL 62
           A  DT++ ++T++++ LA+NPT+  AV +EQ  I   KK GE LTWED  KM  T+ V  
Sbjct: 275 AARDTTASVLTWILKYLADNPTVLEAVTEEQMAIRKDKKEGESLTWEDTKKMPLTYRVIQ 334

Query: 63  ETLRLVAPIFGGFRRALKDIEFGGYLIPKGWQIFWAAPMTQLDGTIFEEPLKFDPSRFEN 122
           ETLR    +   FR A++D+E+ GYLIPKGW++         +  IF +P KFDPSRFE 
Sbjct: 335 ETLRAATILSFTFREAVEDVEYEGYLIPKGWKVLPLFRNIHHNADIFSDPGKFDPSRFE- 394

Query: 123 QASIPPYCFVAFGGGPRICPGYEFGRVETLVTIHYLITQFTWKLLSDELFIR-DPMPIPT 182
             +  P  F+ FG G   CPG E  ++E  V IH+L T++ W ++     I+  P  +P 
Sbjct: 395 -VAPKPNTFMPFGSGIHSCPGNELAKLEISVLIHHLTTKYRWSIVGPSDGIQYGPFALPQ 454

Query: 183 KELPIKI 189
             LPI +
Sbjct: 455 NGLPIAL 459

[Close]

BLAST of Moc11g11420 vs. TAIR 10
Match: AT5G36130.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 145.2 bits (365), Expect = 5.5e-35
Identity = 64/138 (46.38%), Postives = 90/138 (65.22%), Query Frame = 0


Query: 54  MKYTWSVALETLRLVAPIFGGFRRALKDIEFGGYLIPKGWQIFWAAPMTQLDGTIFEEPL 113
           M+Y+W+VA E +R+V P+ G FR A+    F G+ IPKGW+++W+A  T  +   F EP 
Sbjct: 1   MRYSWNVACEVMRIVPPLAGTFREAIDHFSFKGFYIPKGWKLYWSATATHKNPEYFPEPE 60

Query: 114 KFDPSRFENQASIPPYCFVAFGGGPRICPGYEFGRVETLVTIHYLITQFTWKLL--SDEL 173
           KF+PSRFE      PY +V FGGG RICPG E+ R+E L+ +H L+ +F W+ +   +  
Sbjct: 61  KFEPSRFEGSGP-KPYTYVPFGGGSRICPGREYARLEILIFMHNLVKRFKWEKVFPKENK 120

Query: 174 FIRDPMPIPTKELPIKIF 190
            + DP PIP K LPI+IF
Sbjct: 121 LVADPAPIPAKGLPIRIF 137

[Close]

BLAST of Moc11g11420 vs. TAIR 10
Match: AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 141.0 bits (354), Expect = 1.0e-33
Identity = 72/188 (38.30%), Postives = 110/188 (58.51%), Query Frame = 0


Query: 3   AGYDTSSVLITFMVRLLANNPTIHAAVLQEQEEIAGS-KKCGELLTWEDLAKMKYTWSVA 62
           A  DT++ ++T++++ L ++P +   V +EQ  I    KK    ++WED  KM  T  V 
Sbjct: 294 AATDTTASVLTWLLKYLHDHPNLLQEVSREQFSIRQKIKKENRRISWEDTRKMPLTTRVI 353

Query: 63  LETLRLVAPIFGGFRRALKDIEFGGYLIPKGWQIFWAAPMTQLDGTIFEEPLKFDPSRFE 122
            ETLR  + +   FR A++D+E+ GYLIPKGW++             F +P KFDPSRFE
Sbjct: 354 QETLRAASVLSFTFREAVQDVEYDGYLIPKGWKVLPLFRRIHHSSEFFPDPEKFDPSRFE 413

Query: 123 NQASIPPYCFVAFGGGPRICPGYEFGRVETLVTIHYLITQFTWKLLSDELFIR-DPMPIP 182
              +  PY ++ FG G   CPG E  ++E L+ +H+L T F W+++ DE  I+  P P+P
Sbjct: 414 --VAPKPYTYMPFGNGVHSCPGSELAKLEMLILLHHLTTSFRWEVIGDEEGIQYGPFPVP 473

Query: 183 TKELPIKI 189
            K LPI++
Sbjct: 474 KKGLPIRV 479

The following BLAST results are available for this feature:

BLAST of Moc11g11420 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022139355.1	1.3e-97	86.80	LOW QUALITY PROTEIN: cytochrome P450 716B1-like [Momordica charantia]	[more]
XP_038899130.1	9.7e-90	79.41	LOW QUALITY PROTEIN: cytochrome P450 716B1-like [Benincasa hispida]	[more]
XP_008457111.1	5.3e-88	80.31	PREDICTED: cytochrome P450 716B1-like [Cucumis melo] >KAA0047356.1 cytochrome P4...	[more]
XP_004140824.1	3.4e-87	79.90	cytochrome P450 716B1 [Cucumis sativus] >KGN57442.1 hypothetical protein Csa_010...	[more]
KAA8523694.1	5.1e-83	75.66	hypothetical protein F0562_010117 [Nyssa sinensis]	[more]

BLAST of Moc11g11420 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A0S2II38	3.8e-57	53.16	Beta-amyrin 28-monooxygenase OS=Kalopanax septemlobus OX=228393 GN=CYP716A94 PE=...	[more]
I7C6E8	1.9e-56	52.11	Beta-amyrin 28-monooxygenase OS=Panax ginseng OX=4054 GN=CYP716A52v2 PE=1 SV=1	[more]
K4CEE8	6.8e-54	51.58	Beta-amyrin 28-monooxygenase OS=Solanum lycopersicum OX=4081 GN=CYP716A46 PE=1 S...	[more]
Q2MJ20	2.0e-53	51.58	Beta-amyrin 28-monooxygenase OS=Medicago truncatula OX=3880 GN=CYP716A12 PE=1 SV...	[more]
A5BFI4	8.3e-52	50.26	Beta-amyrin 28-monooxygenase OS=Vitis vinifera OX=29760 GN=CYP716A17 PE=1 SV=2	[more]

BLAST of Moc11g11420 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CC35	6.1e-98	86.80	LOW QUALITY PROTEIN: cytochrome P450 716B1-like OS=Momordica charantia OX=3673 G...	[more]
A0A5A7TZG7	2.6e-88	80.31	Cytochrome P450 716B1-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3C4V0	2.6e-88	80.31	cytochrome P450 716B1-like OS=Cucumis melo OX=3656 GN=LOC103496862 PE=3 SV=1	[more]
A0A0A0LA02	1.7e-87	79.90	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G186690 PE=3 SV=1	[more]
A0A2N9F2Q7	5.9e-85	75.65	Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS9063 PE=3 SV=1	[more]

BLAST of Moc11g11420 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G36110.1	6.1e-50	45.55	cytochrome P450, family 716, subfamily A, polypeptide 1	[more]
AT2G42850.1	3.7e-47	44.92	cytochrome P450, family 718	[more]
AT5G45340.1	3.3e-35	41.18	cytochrome P450, family 707, subfamily A, polypeptide 3	[more]
AT5G36130.1	5.5e-35	46.38	Cytochrome P450 superfamily protein	[more]
AT2G29090.1	1.0e-33	38.30	cytochrome P450, family 707, subfamily A, polypeptide 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002397	Cytochrome P450, B-class	PRINTS	PR00359	BP450	coord: 59..70 score: 31.11 coord: 141..152 score: 31.3 coord: 104..119 score: 35.0 coord: 76..103 score: 25.87 coord: 132..141 score: 46.22
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 141..152 score: 31.09 coord: 3..20 score: 32.53 coord: 59..70 score: 34.95 coord: 132..141 score: 52.91
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 1..171 e-value: 2.7E-43 score: 148.4
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 1..190 e-value: 1.0E-56 score: 194.6
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 1..172
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 1..187
None	No IPR available	PANTHER	PTHR24286:SF190	OS07G0518100 PROTEIN	coord: 1..187
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 134..143

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc11g11420.1	Moc11g11420.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016125	sterol metabolic process
biological_process	GO:0006412	translation
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005840	ribosome
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
molecular_function	GO:0003735	structural constituent of ribosome