Moc11g08480 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc11g08480
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptionputrescine-binding periplasmic protein-related
Locationchr11: 6023724 .. 6025701 (+)
RNA-Seq ExpressionMoc11g08480
SyntenyMoc11g08480
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGTACTAACTATGAGTAGATTACTTTTGTCCTACATTGCTGTTTTTGGTATGGAGTTTCGTTTGTTTTATGTACACAAGTATTACTCACTCTGTCTAAATGTTCTAGAAAATAAAGTTGAGCTTTGCTATATCTCAAGGGCAATTTCCTTCAAGTACTCTTGTATCTTCCTTTCTGCAAGAAAGGAAGATTTTTGTGGCAGGCTTGTTTCTTTGTGATTTTACGGGGGATTTGGTTGGGAAGAAACAACAGAATCTTAAGGGTGGAGAGAGATTGGGAGTCTGTTTGGGACTTCATAAGGTTTAATGTCTCTTTATGGGGGCTATTTTGAATTTGTTTTGTAATTACCGGTTAGATTTTATTCTTTTGGATTGGAAGCCCTTTCTTTAGACGGACTTCCTTTTTCTGGGCTGTTATTTTGTATGCCTTTGTTTTTTGTATTTGTTTTCATTTGCCTCAATGAAAAGTTCGGTTTCTTATAAAAAAATAATAATAATAATAAATAAATAAACCTTCAAGTACTCTTAGGTGTCTTAATACTACTGCTGCAGGATTGGTCAGATTTATGGCGCCCTGAACTTCGGGGAAGGATTTCGATGGTCGATTCACCTAGAGAAGTAATTGGTGCTGTTTTAAAGTACATGGGTGCATCCTATAACACGAAAGACATAAGCTTGCAAATTCCTGGTGGTAGAGATGCCGTCCAGCAAAATCTAACTTCACTTGCAAAACAGGTCAGATATGTCTAGTATATTGCAACTACTAAAAGACTATTACTCGCAATAATCGATGCTTTTGGAACTGACTTTTCCTTTAGAATTTGAAAAGGGTTCTTTATATAAGAGTTACTGTTGTTTTCCCCCTCCTGAAAATGCTATTACCATGAATCCCTGGCTCTGGTTTAGCTCTTAGATTACTGATGTATAATTTATTTTGAAAAGAGAAATACCAGAAAAAGAGGATGAGATATCATCCTCTACTAAAGAAAAGGAAAATTACAAGATAAATTAAGGCATCCAGAATTGAAAGAAAAGTGACCCCAAAATCTAAATGCCTTACATTCTTAGACCTACAGAATCTGAGTTGGTAGTGATTTGGCAAATGATACAATCTCAAGTACTCATTTCACCCGGATTTATTAAGCATTACAATTTTGTTCATTGTTATGCACTTGTCTCACACAGGTACGTTTGTTTGATAGCACACACTATCTGAAAGCATTTGCAGTTGGAGATGTGTGGGTTGCGGTTGGATGGAGTAGTGATGTTCTTCCTGTTGTCAAACGCATGTCCAATATTGCAGTGGTCGTCCCGAAGTCTGGATCGAGCTTATGGGCTGATTTATGGGTATATTCAAATTGATCTCTGTCCTCTTTGCAATAGAGATACATAAAAATAAGGTTTGAAGTCACTTCTTAATGTCAACTAGCTTTAGGTAGAAATGCACAATGCTCCTTGATGGTAGAACCCACGTTTTCTTCATTCAAATGTTTCGGGTTCTGTGGTGGTGTTCTTTATCAGATATGTTCTGTTTCTTCAGGCAATTCCTGCAACCTCCAGAATTGAGACACAGCCAGTTGGTGGAAGAGTTAGGGGACCCTCCCCTCTGATCCACCAGTGGATAGAATTTTGCTTGCAGACTGCTCGAGCACTGCCTTTCAAGCAGGAGGTAGTTCCTGGTGCATCGCCAGCCGCCATAGACGGTCCTGTAGCTGTTCCTGAAGAACTGTTTGAAGGTAAACCCAGGCTGGATTCGAACCTTGTGGGCGGAGTACCCCCACTCGATATTCTGGAGAAATGTGAGTTCCTTGAGCCTTTATCTGATTCTAGTTTGGCTGATTACCGATGGTTGGGTGAGAATATGCAGAAGAAACCCGACCATCGTTTGATGGACAGAGTTCATGACGCTGTCTCGTACCTTGTAAGATTTGTTTCTGCAAATCAAAGCTTAAAAAAAACTCAACCGAAACACTAA

mRNA sequence

ATGGAGGATTGGTCAGATTTATGGCGCCCTGAACTTCGGGGAAGGATTTCGATGGTCGATTCACCTAGAGAAGTAATTGGTGCTGTTTTAAAGTACATGGGTGCATCCTATAACACGAAAGACATAAGCTTGCAAATTCCTGGTGGTAGAGATGCCGTCCAGCAAAATCTAACTTCACTTGCAAAACAGGTACGTTTGTTTGATAGCACACACTATCTGAAAGCATTTGCAGTTGGAGATGTGTGGGTTGCGGTTGGATGGAGTAGTGATGTTCTTCCTGTTGTCAAACGCATGTCCAATATTGCAGTGGTCGTCCCGAAGTCTGGATCGAGCTTATGGGCTGATTTATGGGCAATTCCTGCAACCTCCAGAATTGAGACACAGCCAGTTGGTGGAAGAGTTAGGGGACCCTCCCCTCTGATCCACCAGTGGATAGAATTTTGCTTGCAGACTGCTCGAGCACTGCCTTTCAAGCAGGAGGTAGTTCCTGGTGCATCGCCAGCCGCCATAGACGGTCCTGTAGCTGTTCCTGAAGAACTGTTTGAAGGTAAACCCAGGCTGGATTCGAACCTTGTGGGCGGAGTACCCCCACTCGATATTCTGGAGAAATGTGAGTTCCTTGAGCCTTTATCTGATTCTAGTTTGGCTGATTACCGATGGTTGGGTGAGAATATGCAGAAGAAACCCGACCATCGTTTGATGGACAGAGTTCATGACGCTGTCTCGTACCTTGTAAGATTTGTTTCTGCAAATCAAAGCTTAAAAAAAACTCAACCGAAACACTAA

Coding sequence (CDS)

ATGGAGGATTGGTCAGATTTATGGCGCCCTGAACTTCGGGGAAGGATTTCGATGGTCGATTCACCTAGAGAAGTAATTGGTGCTGTTTTAAAGTACATGGGTGCATCCTATAACACGAAAGACATAAGCTTGCAAATTCCTGGTGGTAGAGATGCCGTCCAGCAAAATCTAACTTCACTTGCAAAACAGGTACGTTTGTTTGATAGCACACACTATCTGAAAGCATTTGCAGTTGGAGATGTGTGGGTTGCGGTTGGATGGAGTAGTGATGTTCTTCCTGTTGTCAAACGCATGTCCAATATTGCAGTGGTCGTCCCGAAGTCTGGATCGAGCTTATGGGCTGATTTATGGGCAATTCCTGCAACCTCCAGAATTGAGACACAGCCAGTTGGTGGAAGAGTTAGGGGACCCTCCCCTCTGATCCACCAGTGGATAGAATTTTGCTTGCAGACTGCTCGAGCACTGCCTTTCAAGCAGGAGGTAGTTCCTGGTGCATCGCCAGCCGCCATAGACGGTCCTGTAGCTGTTCCTGAAGAACTGTTTGAAGGTAAACCCAGGCTGGATTCGAACCTTGTGGGCGGAGTACCCCCACTCGATATTCTGGAGAAATGTGAGTTCCTTGAGCCTTTATCTGATTCTAGTTTGGCTGATTACCGATGGTTGGGTGAGAATATGCAGAAGAAACCCGACCATCGTTTGATGGACAGAGTTCATGACGCTGTCTCGTACCTTGTAAGATTTGTTTCTGCAAATCAAAGCTTAAAAAAAACTCAACCGAAACACTAA

Protein sequence

MEDWSDLWRPELRGRISMVDSPREVIGAVLKYMGASYNTKDISLQIPGGRDAVQQNLTSLAKQVRLFDSTHYLKAFAVGDVWVAVGWSSDVLPVVKRMSNIAVVVPKSGSSLWADLWAIPATSRIETQPVGGRVRGPSPLIHQWIEFCLQTARALPFKQEVVPGASPAAIDGPVAVPEELFEGKPRLDSNLVGGVPPLDILEKCEFLEPLSDSSLADYRWLGENMQKKPDHRLMDRVHDAVSYLVRFVSANQSLKKTQPKH
Homology
BLAST of Moc11g08480 vs. NCBI nr
Match: XP_022134391.1 (uncharacterized protein LOC111006660 isoform X1 [Momordica charantia] >XP_022134399.1 uncharacterized protein LOC111006660 isoform X1 [Momordica charantia])

HSP 1 Score: 537.7 bits (1384), Expect = 5.5e-149
Identity = 261/261 (100.00%), Postives = 261/261 (100.00%), Query Frame = 0

Query: 1   MEDWSDLWRPELRGRISMVDSPREVIGAVLKYMGASYNTKDISLQIPGGRDAVQQNLTSL 60
           MEDWSDLWRPELRGRISMVDSPREVIGAVLKYMGASYNTKDISLQIPGGRDAVQQNLTSL
Sbjct: 295 MEDWSDLWRPELRGRISMVDSPREVIGAVLKYMGASYNTKDISLQIPGGRDAVQQNLTSL 354

Query: 61  AKQVRLFDSTHYLKAFAVGDVWVAVGWSSDVLPVVKRMSNIAVVVPKSGSSLWADLWAIP 120
           AKQVRLFDSTHYLKAFAVGDVWVAVGWSSDVLPVVKRMSNIAVVVPKSGSSLWADLWAIP
Sbjct: 355 AKQVRLFDSTHYLKAFAVGDVWVAVGWSSDVLPVVKRMSNIAVVVPKSGSSLWADLWAIP 414

Query: 121 ATSRIETQPVGGRVRGPSPLIHQWIEFCLQTARALPFKQEVVPGASPAAIDGPVAVPEEL 180
           ATSRIETQPVGGRVRGPSPLIHQWIEFCLQTARALPFKQEVVPGASPAAIDGPVAVPEEL
Sbjct: 415 ATSRIETQPVGGRVRGPSPLIHQWIEFCLQTARALPFKQEVVPGASPAAIDGPVAVPEEL 474

Query: 181 FEGKPRLDSNLVGGVPPLDILEKCEFLEPLSDSSLADYRWLGENMQKKPDHRLMDRVHDA 240
           FEGKPRLDSNLVGGVPPLDILEKCEFLEPLSDSSLADYRWLGENMQKKPDHRLMDRVHDA
Sbjct: 475 FEGKPRLDSNLVGGVPPLDILEKCEFLEPLSDSSLADYRWLGENMQKKPDHRLMDRVHDA 534

Query: 241 VSYLVRFVSANQSLKKTQPKH 262
           VSYLVRFVSANQSLKKTQPKH
Sbjct: 535 VSYLVRFVSANQSLKKTQPKH 555

BLAST of Moc11g08480 vs. NCBI nr
Match: XP_023536561.1 (uncharacterized protein LOC111797696 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 470.7 bits (1210), Expect = 8.2e-129
Identity = 224/249 (89.96%), Postives = 238/249 (95.58%), Query Frame = 0

Query: 1   MEDWSDLWRPELRGRISMVDSPREVIGAVLKYMGASYNTKDISLQIPGGRDAVQQNLTSL 60
           MEDWSDLWRPELRGRISMVDSPREVIGAVLKYMGASYNTKDIS QIPGG+DAVQQNLTSL
Sbjct: 283 MEDWSDLWRPELRGRISMVDSPREVIGAVLKYMGASYNTKDISSQIPGGKDAVQQNLTSL 342

Query: 61  AKQVRLFDSTHYLKAFAVGDVWVAVGWSSDVLPVVKRMSNIAVVVPKSGSSLWADLWAIP 120
           AKQVRLFDSTHYLKAFAVGDVWVAVGWSSDVLPVVKRMSNIAVVVPKSGSSLWADLWAIP
Sbjct: 343 AKQVRLFDSTHYLKAFAVGDVWVAVGWSSDVLPVVKRMSNIAVVVPKSGSSLWADLWAIP 402

Query: 121 ATSRIETQPVGGRVRGPSPLIHQWIEFCLQTARALPFKQEVVPGASPAAIDGPVAVPEEL 180
           ATSRIE +PVGGRV+GPSPLIHQWIEFCLQ ARALPFKQEVVPGASPA+I+GP  VP+EL
Sbjct: 403 ATSRIEAEPVGGRVKGPSPLIHQWIEFCLQPARALPFKQEVVPGASPASIEGPEVVPKEL 462

Query: 181 FEGKPRLDSNLVGGVPPLDILEKCEFLEPLSDSSLADYRWLGENMQKKPDHRLMDRVHDA 240
           FEGKP+LD+NL+GGVPPLDIL+KCEFLEPLS+S+LAD+RW   NMQKKP+HRLMDRVH A
Sbjct: 463 FEGKPKLDTNLIGGVPPLDILDKCEFLEPLSESTLADFRWFAANMQKKPNHRLMDRVHQA 522

Query: 241 VSYLVRFVS 250
           VS LV FVS
Sbjct: 523 VSSLVGFVS 531

BLAST of Moc11g08480 vs. NCBI nr
Match: XP_022936759.1 (uncharacterized protein LOC111443255 [Cucurbita moschata])

HSP 1 Score: 469.5 bits (1207), Expect = 1.8e-128
Identity = 224/249 (89.96%), Postives = 237/249 (95.18%), Query Frame = 0

Query: 1   MEDWSDLWRPELRGRISMVDSPREVIGAVLKYMGASYNTKDISLQIPGGRDAVQQNLTSL 60
           MEDWSDLWRPELRGRISMVDSPREVIGAVLKYMGASYNTKDIS QIPGG+DAVQQNLTSL
Sbjct: 283 MEDWSDLWRPELRGRISMVDSPREVIGAVLKYMGASYNTKDISSQIPGGKDAVQQNLTSL 342

Query: 61  AKQVRLFDSTHYLKAFAVGDVWVAVGWSSDVLPVVKRMSNIAVVVPKSGSSLWADLWAIP 120
           AKQVRLFDSTHYLKAFAVGDVWVAVGWSSDVLPVVKRMSNIAVVVPKSGSSLWADLWAIP
Sbjct: 343 AKQVRLFDSTHYLKAFAVGDVWVAVGWSSDVLPVVKRMSNIAVVVPKSGSSLWADLWAIP 402

Query: 121 ATSRIETQPVGGRVRGPSPLIHQWIEFCLQTARALPFKQEVVPGASPAAIDGPVAVPEEL 180
           ATSRIE +PVGGRV+GPSPLIHQWIEFCLQ ARALPFKQEVVPGASPA+I+GP  VP+EL
Sbjct: 403 ATSRIEAEPVGGRVKGPSPLIHQWIEFCLQPARALPFKQEVVPGASPASIEGPEVVPKEL 462

Query: 181 FEGKPRLDSNLVGGVPPLDILEKCEFLEPLSDSSLADYRWLGENMQKKPDHRLMDRVHDA 240
           FEGKP+LD+NL+GGVPPLDILEKCEFLEPLS+S+ AD+RW   NMQKKP+HRLMDRVH A
Sbjct: 463 FEGKPKLDTNLIGGVPPLDILEKCEFLEPLSESTSADFRWFAANMQKKPNHRLMDRVHQA 522

Query: 241 VSYLVRFVS 250
           VS LV FVS
Sbjct: 523 VSSLVGFVS 531

BLAST of Moc11g08480 vs. NCBI nr
Match: XP_022976753.1 (uncharacterized protein LOC111477037 [Cucurbita maxima])

HSP 1 Score: 469.5 bits (1207), Expect = 1.8e-128
Identity = 223/249 (89.56%), Postives = 238/249 (95.58%), Query Frame = 0

Query: 1   MEDWSDLWRPELRGRISMVDSPREVIGAVLKYMGASYNTKDISLQIPGGRDAVQQNLTSL 60
           MEDWSDLWRPELRGRISMVDSPREVIGAVLKYMGASYNTKDIS QIPGG+DAVQQ+LTSL
Sbjct: 283 MEDWSDLWRPELRGRISMVDSPREVIGAVLKYMGASYNTKDISSQIPGGKDAVQQSLTSL 342

Query: 61  AKQVRLFDSTHYLKAFAVGDVWVAVGWSSDVLPVVKRMSNIAVVVPKSGSSLWADLWAIP 120
           +KQVRLFDSTHYLKAFAVGDVWVAVGWSSDVLPVVKRMSNIAVVVPKSGSSLWADLWAIP
Sbjct: 343 SKQVRLFDSTHYLKAFAVGDVWVAVGWSSDVLPVVKRMSNIAVVVPKSGSSLWADLWAIP 402

Query: 121 ATSRIETQPVGGRVRGPSPLIHQWIEFCLQTARALPFKQEVVPGASPAAIDGPVAVPEEL 180
           ATSRIE +PVGGRV+GPSPLIHQWIEFCLQ ARALPFKQEVVPGASPA+I+GP  VP+EL
Sbjct: 403 ATSRIEAEPVGGRVKGPSPLIHQWIEFCLQPARALPFKQEVVPGASPASIEGPEVVPKEL 462

Query: 181 FEGKPRLDSNLVGGVPPLDILEKCEFLEPLSDSSLADYRWLGENMQKKPDHRLMDRVHDA 240
           FEGKP+LD+NL+GGVPPLDILEKCEFLEPLS+S+LAD+RW  ENMQKKP+HRLMDRVH A
Sbjct: 463 FEGKPKLDTNLIGGVPPLDILEKCEFLEPLSESTLADFRWFAENMQKKPNHRLMDRVHQA 522

Query: 241 VSYLVRFVS 250
           VS LV F S
Sbjct: 523 VSSLVGFAS 531

BLAST of Moc11g08480 vs. NCBI nr
Match: XP_038898464.1 (spermidine-binding periplasmic protein SpuE isoform X1 [Benincasa hispida])

HSP 1 Score: 468.4 bits (1204), Expect = 4.1e-128
Identity = 226/249 (90.76%), Postives = 238/249 (95.58%), Query Frame = 0

Query: 1   MEDWSDLWRPELRGRISMVDSPREVIGAVLKYMGASYNTKDISLQIPGGRDAVQQNLTSL 60
           +EDWSDLWRPELRGRISMV+SPREVIGAVLKYMGASYNTK+IS QIPGGRDAVQQNL SL
Sbjct: 287 VEDWSDLWRPELRGRISMVNSPREVIGAVLKYMGASYNTKNISSQIPGGRDAVQQNLASL 346

Query: 61  AKQVRLFDSTHYLKAFAVGDVWVAVGWSSDVLPVVKRMSNIAVVVPKSGSSLWADLWAIP 120
           AKQVRLFDSTHYLKAFAVGDVWVAVGWSSDVLPVVKRMSN+AVVVPKSGSSLWADLWAIP
Sbjct: 347 AKQVRLFDSTHYLKAFAVGDVWVAVGWSSDVLPVVKRMSNVAVVVPKSGSSLWADLWAIP 406

Query: 121 ATSRIETQPVGGRVRGPSPLIHQWIEFCLQTARALPFKQEVVPGASPAAIDGPVAVPEEL 180
           ATSRIET+PVGGRVRGPSPLIHQWIEFCLQTARALPFKQEVVPGA PAAI+GPV VP+EL
Sbjct: 407 ATSRIETEPVGGRVRGPSPLIHQWIEFCLQTARALPFKQEVVPGALPAAIEGPVVVPKEL 466

Query: 181 FEGKPRLDSNLVGGVPPLDILEKCEFLEPLSDSSLADYRWLGENMQKKPDHRLMDRVHDA 240
           FEGKP+LD+NL+GGVPP DILEKCEFLEPLSDS+L DYRWL  NMQ KP+HRLMDRVH A
Sbjct: 467 FEGKPKLDTNLIGGVPPSDILEKCEFLEPLSDSTLTDYRWLVSNMQ-KPEHRLMDRVHQA 526

Query: 241 VSYLVRFVS 250
           VS LVRFVS
Sbjct: 527 VSSLVRFVS 534

BLAST of Moc11g08480 vs. ExPASy Swiss-Prot
Match: Q9I6J0 (Spermidine-binding periplasmic protein SpuE OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) OX=208964 GN=spuE PE=1 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 9.1e-14
Identity = 60/217 (27.65%), Postives = 97/217 (44.70%), Query Frame = 0

Query: 1   MEDWSDLWRPE-----LRGRISMVDSPREVIGAVLKYMGASYNTKDISLQIPGGRDAVQQ 60
           ++ W+ L+ PE      +  ++ +DS  E++ A L Y+G   NT D     P      ++
Sbjct: 153 IDSWAILFEPENMKKLAKCGVAFMDSGDEMLPAALNYLGLDPNTHD-----PKDYKKAEE 212

Query: 61  NLTSLAKQVRLFDSTHYLKAFAVGDVWVAVGWSSDVLPVVKRMS------NIAVVVPKSG 120
            LT +   V  F S+ Y+   A G++ VA G+S DV     R        +I  V+PK G
Sbjct: 213 VLTKVRPYVSYFHSSKYISDLANGNICVAFGYSGDVFQAAARAEEAGKGIDIQYVIPKEG 272

Query: 121 SSLWADLWAIPATSRIETQPVGGRVRGPSPLIHQWIEFCLQTARALPFKQEV-----VPG 180
           ++LW DL AIPA ++                 + +I++ L+          V     +PG
Sbjct: 273 ANLWFDLMAIPADAKAADN------------AYAFIDYLLRPEVIAKVSDYVGYANAIPG 332

Query: 181 ASPAAIDGPVAVPEELFEGKPRLDSNLVGGVPPLDIL 202
           A P  +D  V+  EE++  +  LD   V  V P  +L
Sbjct: 333 ARP-LMDKSVSDSEEVYPPQAVLDKLYVSAVLPAKVL 351

BLAST of Moc11g08480 vs. ExPASy Swiss-Prot
Match: P45168 (Spermidine/putrescine-binding periplasmic protein 1 OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) OX=71421 GN=potD-B PE=3 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 1.7e-12
Identity = 40/121 (33.06%), Postives = 59/121 (48.76%), Query Frame = 0

Query: 4   WSDLWRPELRGRISMVDSPREVIGAVLKYMGASYNTKDISLQIPGGRDAVQQNLTSLAKQ 63
           W+DLW+PE   ++ ++D  REV    L  +G   NT+D     P       + L  L   
Sbjct: 163 WADLWKPEFANKVQLLDDAREVFNIALLKIGQDPNTQD-----PAIIKQAYEELLKLRPN 222

Query: 64  VRLFDSTHYLKAFAVGDVWVAVGWSSDVLPVVKRMSNIAVVVPKSGSSLWADLWAIPATS 123
           V  F+S +   +F  G+V V   W+  V    K  + + +V PK G  LW D  AIPAT+
Sbjct: 223 VLSFNSDNPANSFISGEVEVGQLWNGSVRIAKKEKAPLNMVFPKEGPVLWVDTLAIPATA 278

Query: 124 R 125
           +
Sbjct: 283 K 278

BLAST of Moc11g08480 vs. ExPASy Swiss-Prot
Match: P0AFK9 (Spermidine/putrescine-binding periplasmic protein OS=Escherichia coli (strain K12) OX=83333 GN=potD PE=1 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 1.9e-11
Identity = 39/121 (32.23%), Postives = 58/121 (47.93%), Query Frame = 0

Query: 4   WSDLWRPELRGRISMVDSPREVIGAVLKYMGASYNTKDISLQIPGGRDAVQQNLTSLAKQ 63
           W+DLW+PE +G + + D  REV    L+ +G S NT D     P   +A    L  L   
Sbjct: 151 WADLWKPEYKGSLLLTDDAREVFQMALRKLGYSGNTTD-----PKEIEAAYNELKKLMPN 210

Query: 64  VRLFDSTHYLKAFAVGDVWVAVGWSSDVLPVVKRMSNIAVVVPKSGSSLWADLWAIPATS 123
           V  F+S +    +  G+V + + W+       +  + I VV PK G   W D  AIPA +
Sbjct: 211 VAAFNSDNPANPYMEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANA 266

Query: 124 R 125
           +
Sbjct: 271 K 266

BLAST of Moc11g08480 vs. ExPASy Swiss-Prot
Match: P0AFL0 (Spermidine/putrescine-binding periplasmic protein OS=Shigella flexneri OX=623 GN=potD PE=3 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 1.9e-11
Identity = 39/121 (32.23%), Postives = 58/121 (47.93%), Query Frame = 0

Query: 4   WSDLWRPELRGRISMVDSPREVIGAVLKYMGASYNTKDISLQIPGGRDAVQQNLTSLAKQ 63
           W+DLW+PE +G + + D  REV    L+ +G S NT D     P   +A    L  L   
Sbjct: 151 WADLWKPEYKGSLLLTDDAREVFQMALRKLGYSGNTTD-----PKEIEAAYNELKKLMPN 210

Query: 64  VRLFDSTHYLKAFAVGDVWVAVGWSSDVLPVVKRMSNIAVVVPKSGSSLWADLWAIPATS 123
           V  F+S +    +  G+V + + W+       +  + I VV PK G   W D  AIPA +
Sbjct: 211 VAAFNSDNPANPYMEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANA 266

Query: 124 R 125
           +
Sbjct: 271 K 266

BLAST of Moc11g08480 vs. ExPASy Swiss-Prot
Match: P0A2C8 (Spermidine/putrescine-binding periplasmic protein OS=Salmonella typhi OX=90370 GN=potD PE=3 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 5.5e-11
Identity = 37/121 (30.58%), Postives = 58/121 (47.93%), Query Frame = 0

Query: 4   WSDLWRPELRGRISMVDSPREVIGAVLKYMGASYNTKDISLQIPGGRDAVQQNLTSLAKQ 63
           W+DLW+PE +  + + D  REV    L+ +G S NT D     P   +A  + L  L   
Sbjct: 151 WADLWKPEYKNSLLLTDDAREVFQMALRKLGYSGNTTD-----PKEIEAAYEELKKLMPN 210

Query: 64  VRLFDSTHYLKAFAVGDVWVAVGWSSDVLPVVKRMSNIAVVVPKSGSSLWADLWAIPATS 123
           V  F+S +    +  G+V + + W+       +  + + VV PK G   W D  AIPA +
Sbjct: 211 VAAFNSDNPANPYMEGEVNLGMVWNGSAFVARQAGTPLEVVWPKEGGIFWMDSLAIPANA 266

Query: 124 R 125
           +
Sbjct: 271 K 266

BLAST of Moc11g08480 vs. ExPASy TrEMBL
Match: A0A6J1BXP6 (uncharacterized protein LOC111006660 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111006660 PE=4 SV=1)

HSP 1 Score: 537.7 bits (1384), Expect = 2.6e-149
Identity = 261/261 (100.00%), Postives = 261/261 (100.00%), Query Frame = 0

Query: 1   MEDWSDLWRPELRGRISMVDSPREVIGAVLKYMGASYNTKDISLQIPGGRDAVQQNLTSL 60
           MEDWSDLWRPELRGRISMVDSPREVIGAVLKYMGASYNTKDISLQIPGGRDAVQQNLTSL
Sbjct: 295 MEDWSDLWRPELRGRISMVDSPREVIGAVLKYMGASYNTKDISLQIPGGRDAVQQNLTSL 354

Query: 61  AKQVRLFDSTHYLKAFAVGDVWVAVGWSSDVLPVVKRMSNIAVVVPKSGSSLWADLWAIP 120
           AKQVRLFDSTHYLKAFAVGDVWVAVGWSSDVLPVVKRMSNIAVVVPKSGSSLWADLWAIP
Sbjct: 355 AKQVRLFDSTHYLKAFAVGDVWVAVGWSSDVLPVVKRMSNIAVVVPKSGSSLWADLWAIP 414

Query: 121 ATSRIETQPVGGRVRGPSPLIHQWIEFCLQTARALPFKQEVVPGASPAAIDGPVAVPEEL 180
           ATSRIETQPVGGRVRGPSPLIHQWIEFCLQTARALPFKQEVVPGASPAAIDGPVAVPEEL
Sbjct: 415 ATSRIETQPVGGRVRGPSPLIHQWIEFCLQTARALPFKQEVVPGASPAAIDGPVAVPEEL 474

Query: 181 FEGKPRLDSNLVGGVPPLDILEKCEFLEPLSDSSLADYRWLGENMQKKPDHRLMDRVHDA 240
           FEGKPRLDSNLVGGVPPLDILEKCEFLEPLSDSSLADYRWLGENMQKKPDHRLMDRVHDA
Sbjct: 475 FEGKPRLDSNLVGGVPPLDILEKCEFLEPLSDSSLADYRWLGENMQKKPDHRLMDRVHDA 534

Query: 241 VSYLVRFVSANQSLKKTQPKH 262
           VSYLVRFVSANQSLKKTQPKH
Sbjct: 535 VSYLVRFVSANQSLKKTQPKH 555

BLAST of Moc11g08480 vs. ExPASy TrEMBL
Match: A0A6J1IN39 (uncharacterized protein LOC111477037 OS=Cucurbita maxima OX=3661 GN=LOC111477037 PE=4 SV=1)

HSP 1 Score: 469.5 bits (1207), Expect = 8.8e-129
Identity = 223/249 (89.56%), Postives = 238/249 (95.58%), Query Frame = 0

Query: 1   MEDWSDLWRPELRGRISMVDSPREVIGAVLKYMGASYNTKDISLQIPGGRDAVQQNLTSL 60
           MEDWSDLWRPELRGRISMVDSPREVIGAVLKYMGASYNTKDIS QIPGG+DAVQQ+LTSL
Sbjct: 283 MEDWSDLWRPELRGRISMVDSPREVIGAVLKYMGASYNTKDISSQIPGGKDAVQQSLTSL 342

Query: 61  AKQVRLFDSTHYLKAFAVGDVWVAVGWSSDVLPVVKRMSNIAVVVPKSGSSLWADLWAIP 120
           +KQVRLFDSTHYLKAFAVGDVWVAVGWSSDVLPVVKRMSNIAVVVPKSGSSLWADLWAIP
Sbjct: 343 SKQVRLFDSTHYLKAFAVGDVWVAVGWSSDVLPVVKRMSNIAVVVPKSGSSLWADLWAIP 402

Query: 121 ATSRIETQPVGGRVRGPSPLIHQWIEFCLQTARALPFKQEVVPGASPAAIDGPVAVPEEL 180
           ATSRIE +PVGGRV+GPSPLIHQWIEFCLQ ARALPFKQEVVPGASPA+I+GP  VP+EL
Sbjct: 403 ATSRIEAEPVGGRVKGPSPLIHQWIEFCLQPARALPFKQEVVPGASPASIEGPEVVPKEL 462

Query: 181 FEGKPRLDSNLVGGVPPLDILEKCEFLEPLSDSSLADYRWLGENMQKKPDHRLMDRVHDA 240
           FEGKP+LD+NL+GGVPPLDILEKCEFLEPLS+S+LAD+RW  ENMQKKP+HRLMDRVH A
Sbjct: 463 FEGKPKLDTNLIGGVPPLDILEKCEFLEPLSESTLADFRWFAENMQKKPNHRLMDRVHQA 522

Query: 241 VSYLVRFVS 250
           VS LV F S
Sbjct: 523 VSSLVGFAS 531

BLAST of Moc11g08480 vs. ExPASy TrEMBL
Match: A0A6J1F970 (uncharacterized protein LOC111443255 OS=Cucurbita moschata OX=3662 GN=LOC111443255 PE=4 SV=1)

HSP 1 Score: 469.5 bits (1207), Expect = 8.8e-129
Identity = 224/249 (89.96%), Postives = 237/249 (95.18%), Query Frame = 0

Query: 1   MEDWSDLWRPELRGRISMVDSPREVIGAVLKYMGASYNTKDISLQIPGGRDAVQQNLTSL 60
           MEDWSDLWRPELRGRISMVDSPREVIGAVLKYMGASYNTKDIS QIPGG+DAVQQNLTSL
Sbjct: 283 MEDWSDLWRPELRGRISMVDSPREVIGAVLKYMGASYNTKDISSQIPGGKDAVQQNLTSL 342

Query: 61  AKQVRLFDSTHYLKAFAVGDVWVAVGWSSDVLPVVKRMSNIAVVVPKSGSSLWADLWAIP 120
           AKQVRLFDSTHYLKAFAVGDVWVAVGWSSDVLPVVKRMSNIAVVVPKSGSSLWADLWAIP
Sbjct: 343 AKQVRLFDSTHYLKAFAVGDVWVAVGWSSDVLPVVKRMSNIAVVVPKSGSSLWADLWAIP 402

Query: 121 ATSRIETQPVGGRVRGPSPLIHQWIEFCLQTARALPFKQEVVPGASPAAIDGPVAVPEEL 180
           ATSRIE +PVGGRV+GPSPLIHQWIEFCLQ ARALPFKQEVVPGASPA+I+GP  VP+EL
Sbjct: 403 ATSRIEAEPVGGRVKGPSPLIHQWIEFCLQPARALPFKQEVVPGASPASIEGPEVVPKEL 462

Query: 181 FEGKPRLDSNLVGGVPPLDILEKCEFLEPLSDSSLADYRWLGENMQKKPDHRLMDRVHDA 240
           FEGKP+LD+NL+GGVPPLDILEKCEFLEPLS+S+ AD+RW   NMQKKP+HRLMDRVH A
Sbjct: 463 FEGKPKLDTNLIGGVPPLDILEKCEFLEPLSESTSADFRWFAANMQKKPNHRLMDRVHQA 522

Query: 241 VSYLVRFVS 250
           VS LV FVS
Sbjct: 523 VSSLVGFVS 531

BLAST of Moc11g08480 vs. ExPASy TrEMBL
Match: A0A5A7UA94 (Spermidine-binding periplasmic protein SpuE OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold113G00990 PE=4 SV=1)

HSP 1 Score: 468.0 bits (1203), Expect = 2.6e-128
Identity = 225/249 (90.36%), Postives = 237/249 (95.18%), Query Frame = 0

Query: 1   MEDWSDLWRPELRGRISMVDSPREVIGAVLKYMGASYNTKDISLQIPGGRDAVQQNLTSL 60
           MEDWSDLWRPEL+GRISMVDSPREVIGAVLKYMGASYNT++IS QIPGGRDA+QQNLTSL
Sbjct: 289 MEDWSDLWRPELQGRISMVDSPREVIGAVLKYMGASYNTENISSQIPGGRDAIQQNLTSL 348

Query: 61  AKQVRLFDSTHYLKAFAVGDVWVAVGWSSDVLPVVKRMSNIAVVVPKSGSSLWADLWAIP 120
           AKQVRLFDSTHYLKAFAVGDVWVAVGWSSDVLPVVKRMSNIAVVVPKSGSSLWADLWAIP
Sbjct: 349 AKQVRLFDSTHYLKAFAVGDVWVAVGWSSDVLPVVKRMSNIAVVVPKSGSSLWADLWAIP 408

Query: 121 ATSRIETQPVGGRVRGPSPLIHQWIEFCLQTARALPFKQEVVPGASPAAIDGPVAVPEEL 180
           ATSRIET+P+GGRVRGPSPLIHQWIEFCLQTARALPFKQEVVPGASPAAI+GPV VP+EL
Sbjct: 409 ATSRIETEPIGGRVRGPSPLIHQWIEFCLQTARALPFKQEVVPGASPAAIEGPVVVPKEL 468

Query: 181 FEGKPRLDSNLVGGVPPLDILEKCEFLEPLSDSSLADYRWLGENMQKKPDHRLMDRVHDA 240
           FEGKP+LD+NL+ GVPP DILEKCEFL PLSDS+LADYRWL  NMQ KP HRLMDRVH A
Sbjct: 469 FEGKPKLDTNLISGVPPSDILEKCEFLHPLSDSTLADYRWLAANMQ-KPKHRLMDRVHQA 528

Query: 241 VSYLVRFVS 250
            S LVRFVS
Sbjct: 529 ASSLVRFVS 536

BLAST of Moc11g08480 vs. ExPASy TrEMBL
Match: A0A1S3BAS3 (spermidine-binding periplasmic protein SpuE OS=Cucumis melo OX=3656 GN=LOC103487640 PE=4 SV=1)

HSP 1 Score: 468.0 bits (1203), Expect = 2.6e-128
Identity = 225/249 (90.36%), Postives = 237/249 (95.18%), Query Frame = 0

Query: 1   MEDWSDLWRPELRGRISMVDSPREVIGAVLKYMGASYNTKDISLQIPGGRDAVQQNLTSL 60
           MEDWSDLWRPEL+GRISMVDSPREVIGAVLKYMGASYNT++IS QIPGGRDA+QQNLTSL
Sbjct: 289 MEDWSDLWRPELQGRISMVDSPREVIGAVLKYMGASYNTENISSQIPGGRDAIQQNLTSL 348

Query: 61  AKQVRLFDSTHYLKAFAVGDVWVAVGWSSDVLPVVKRMSNIAVVVPKSGSSLWADLWAIP 120
           AKQVRLFDSTHYLKAFAVGDVWVAVGWSSDVLPVVKRMSNIAVVVPKSGSSLWADLWAIP
Sbjct: 349 AKQVRLFDSTHYLKAFAVGDVWVAVGWSSDVLPVVKRMSNIAVVVPKSGSSLWADLWAIP 408

Query: 121 ATSRIETQPVGGRVRGPSPLIHQWIEFCLQTARALPFKQEVVPGASPAAIDGPVAVPEEL 180
           ATSRIET+P+GGRVRGPSPLIHQWIEFCLQTARALPFKQEVVPGASPAAI+GPV VP+EL
Sbjct: 409 ATSRIETEPIGGRVRGPSPLIHQWIEFCLQTARALPFKQEVVPGASPAAIEGPVVVPKEL 468

Query: 181 FEGKPRLDSNLVGGVPPLDILEKCEFLEPLSDSSLADYRWLGENMQKKPDHRLMDRVHDA 240
           FEGKP+LD+NL+ GVPP DILEKCEFL PLSDS+LADYRWL  NMQ KP HRLMDRVH A
Sbjct: 469 FEGKPKLDTNLISGVPPSDILEKCEFLHPLSDSTLADYRWLAANMQ-KPKHRLMDRVHQA 528

Query: 241 VSYLVRFVS 250
            S LVRFVS
Sbjct: 529 ASSLVRFVS 536

BLAST of Moc11g08480 vs. TAIR 10
Match: AT1G31410.1 (putrescine-binding periplasmic protein-related )

HSP 1 Score: 338.2 bits (866), Expect = 5.9e-93
Identity = 163/250 (65.20%), Postives = 204/250 (81.60%), Query Frame = 0

Query: 1   MEDWSDLWRPELRGRISMVDSPREVIGAVLKYMGASYNTKDISLQIPGGRDAVQQNLTSL 60
           +EDW+DLWRPEL GRI+MV+SPREV+GAVLKYM ASYNT D+  Q+PGGR AV++NL SL
Sbjct: 261 IEDWADLWRPELAGRIAMVNSPREVVGAVLKYMRASYNTTDLDSQVPGGRLAVEKNLASL 320

Query: 61  AKQVRLFDSTHYLKAFAVGDVWVAVGWSSDVLPVVKRMSNIAVVVPKSGSSLWADLWAIP 120
            KQ+RLFDS +YLKAF VGDVWV VGWSSDV+PV KRMSN+ V+VPKSG++LWADLWAIP
Sbjct: 321 MKQIRLFDSNNYLKAFNVGDVWVTVGWSSDVIPVAKRMSNVTVIVPKSGATLWADLWAIP 380

Query: 121 ATSRI--ETQPVGGRVRGPSPLIHQWIEFCLQTARALPFKQEVVPGASPAAIDGP-VAVP 180
           A S    E +  GGRVRGPSPLI+QWIEFCLQ AR+LPF +EV+PGASP+A+DGP V  P
Sbjct: 381 AVSDSGKEAEQRGGRVRGPSPLINQWIEFCLQPARSLPFTREVIPGASPSALDGPLVTEP 440

Query: 181 EELFEGKPRLDSNLVGGVPPLDILEKCEFLEPLSDSSLADYRWLGENMQKKPDH-RLMDR 240
           E+  + + +LD+NLV GVPP +IL KCEFLEPL +++L++YR L E ++K+     L+++
Sbjct: 441 EKTKKDRTKLDTNLVTGVPPPEILSKCEFLEPLPEATLSEYRLLIETVRKQSQRPGLVEK 500

Query: 241 VHDAVSYLVR 247
           + D VS  VR
Sbjct: 501 LKDIVSIKVR 510

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022134391.15.5e-149100.00uncharacterized protein LOC111006660 isoform X1 [Momordica charantia] >XP_022134... [more]
XP_023536561.18.2e-12989.96uncharacterized protein LOC111797696 [Cucurbita pepo subsp. pepo][more]
XP_022936759.11.8e-12889.96uncharacterized protein LOC111443255 [Cucurbita moschata][more]
XP_022976753.11.8e-12889.56uncharacterized protein LOC111477037 [Cucurbita maxima][more]
XP_038898464.14.1e-12890.76spermidine-binding periplasmic protein SpuE isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9I6J09.1e-1427.65Spermidine-binding periplasmic protein SpuE OS=Pseudomonas aeruginosa (strain AT... [more]
P451681.7e-1233.06Spermidine/putrescine-binding periplasmic protein 1 OS=Haemophilus influenzae (s... [more]
P0AFK91.9e-1132.23Spermidine/putrescine-binding periplasmic protein OS=Escherichia coli (strain K1... [more]
P0AFL01.9e-1132.23Spermidine/putrescine-binding periplasmic protein OS=Shigella flexneri OX=623 GN... [more]
P0A2C85.5e-1130.58Spermidine/putrescine-binding periplasmic protein OS=Salmonella typhi OX=90370 G... [more]
Match NameE-valueIdentityDescription
A0A6J1BXP62.6e-149100.00uncharacterized protein LOC111006660 isoform X1 OS=Momordica charantia OX=3673 G... [more]
A0A6J1IN398.8e-12989.56uncharacterized protein LOC111477037 OS=Cucurbita maxima OX=3661 GN=LOC111477037... [more]
A0A6J1F9708.8e-12989.96uncharacterized protein LOC111443255 OS=Cucurbita moschata OX=3662 GN=LOC1114432... [more]
A0A5A7UA942.6e-12890.36Spermidine-binding periplasmic protein SpuE OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3BAS32.6e-12890.36spermidine-binding periplasmic protein SpuE OS=Cucumis melo OX=3656 GN=LOC103487... [more]
Match NameE-valueIdentityDescription
AT1G31410.15.9e-9365.20putrescine-binding periplasmic protein-related [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001188Bacterial periplasmic spermidine/putrescine-binding proteinPRINTSPR00909SPERMDNBNDNGcoord: 2..16
score: 37.14
coord: 106..125
score: 44.29
coord: 20..39
score: 40.71
NoneNo IPR availablePFAMPF13343SBP_bac_6coord: 2..153
e-value: 5.7E-22
score: 78.3
NoneNo IPR availableGENE3D3.40.190.10coord: 1..132
e-value: 5.6E-37
score: 129.3
NoneNo IPR availablePANTHERPTHR30222:SF17SPERMIDINE/PUTRESCINE-BINDING PERIPLASMIC PROTEINcoord: 2..238
NoneNo IPR availablePANTHERPTHR30222SPERMIDINE/PUTRESCINE-BINDING PERIPLASMIC PROTEINcoord: 2..238
NoneNo IPR availableSUPERFAMILY53850Periplasmic binding protein-like IIcoord: 2..124

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc11g08480.1Moc11g08480.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009657 plastid organization
biological_process GO:0015846 polyamine transport
cellular_component GO:0009507 chloroplast
cellular_component GO:0042597 periplasmic space
molecular_function GO:0019808 polyamine binding