Moc11g07780 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc11g07780
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	EF hand calcium-binding family protein
Location	chr11: 5493371 .. 5493868 (+)
RNA-Seq Expression	Moc11g07780
Synteny	Moc11g07780

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCCAAGCAGGATCTCTATCGGCCGAGACCGAGAGTCTCAGCCACATGTTTAGCCTAATAGAAGCATTCCGAGCATTCGACGCAGACAATGATGGGCTGATCACGGCACCGGAGGTCGGGGGGATCATGGGTTCGCTCGGGTACAACCTGAGCGAGGAAGACGTGAATTTGATGATGGAGGAAGGAGACGTGGACAAAGACGGGCTGCTGAGCATGGGGGAGTTTCTGGAGATGAATGCAAGGAGTGTGGAGGTGGGGGAGGTGATGGGGAGCTATCTGAAGATTGCTTATGAGGCTCTGAAGGCAGATGGGGATGATCTGGTGAGCGGGGAAGAGCTTTATGATGTGTTTTTGAATGTGGGATTGGAGGTTTCTGTTGAGGATTGTAATGCTATAGTGGCTTCCATGGATGGGGATGAAGATGGAGCTGTGTTTCTTGAGGATTTGAAACTTATTGTTGATTCTCTTCACCCCAACTCCAACCCCCAACTCTAG

mRNA sequence

ATGGCCCAAGCAGGATCTCTATCGGCCGAGACCGAGAGTCTCAGCCACATGTTTAGCCTAATAGAAGCATTCCGAGCATTCGACGCAGACAATGATGGGCTGATCACGGCACCGGAGGTCGGGGGGATCATGGGTTCGCTCGGGTACAACCTGAGCGAGGAAGACGTGAATTTGATGATGGAGGAAGGAGACGTGGACAAAGACGGGCTGCTGAGCATGGGGGAGTTTCTGGAGATGAATGCAAGGAGTGTGGAGGTGGGGGAGGTGATGGGGAGCTATCTGAAGATTGCTTATGAGGCTCTGAAGGCAGATGGGGATGATCTGGTGAGCGGGGAAGAGCTTTATGATGTGTTTTTGAATGTGGGATTGGAGGTTTCTGTTGAGGATTGTAATGCTATAGTGGCTTCCATGGATGGGGATGAAGATGGAGCTGTGTTTCTTGAGGATTTGAAACTTATTGTTGATTCTCTTCACCCCAACTCCAACCCCCAACTCTAG

Coding sequence (CDS)

ATGGCCCAAGCAGGATCTCTATCGGCCGAGACCGAGAGTCTCAGCCACATGTTTAGCCTAATAGAAGCATTCCGAGCATTCGACGCAGACAATGATGGGCTGATCACGGCACCGGAGGTCGGGGGGATCATGGGTTCGCTCGGGTACAACCTGAGCGAGGAAGACGTGAATTTGATGATGGAGGAAGGAGACGTGGACAAAGACGGGCTGCTGAGCATGGGGGAGTTTCTGGAGATGAATGCAAGGAGTGTGGAGGTGGGGGAGGTGATGGGGAGCTATCTGAAGATTGCTTATGAGGCTCTGAAGGCAGATGGGGATGATCTGGTGAGCGGGGAAGAGCTTTATGATGTGTTTTTGAATGTGGGATTGGAGGTTTCTGTTGAGGATTGTAATGCTATAGTGGCTTCCATGGATGGGGATGAAGATGGAGCTGTGTTTCTTGAGGATTTGAAACTTATTGTTGATTCTCTTCACCCCAACTCCAACCCCCAACTCTAG

Protein sequence

MAQAGSLSAETESLSHMFSLIEAFRAFDADNDGLITAPEVGGIMGSLGYNLSEEDVNLMMEEGDVDKDGLLSMGEFLEMNARSVEVGEVMGSYLKIAYEALKADGDDLVSGEELYDVFLNVGLEVSVEDCNAIVASMDGDEDGAVFLEDLKLIVDSLHPNSNPQL

Homology

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BLAST of Moc11g07780 vs. NCBI nr
Match: XP_022142920.1 (probable calcium-binding protein CML29 [Momordica charantia])

HSP 1 Score: 320.5 bits (820), Expect = 8.7e-84
Identity = 165/165 (100.00%), Postives = 165/165 (100.00%), Query Frame = 0


Query: 1   MAQAGSLSAETESLSHMFSLIEAFRAFDADNDGLITAPEVGGIMGSLGYNLSEEDVNLMM 60
           MAQAGSLSAETESLSHMFSLIEAFRAFDADNDGLITAPEVGGIMGSLGYNLSEEDVNLMM
Sbjct: 1   MAQAGSLSAETESLSHMFSLIEAFRAFDADNDGLITAPEVGGIMGSLGYNLSEEDVNLMM 60

Query: 61  EEGDVDKDGLLSMGEFLEMNARSVEVGEVMGSYLKIAYEALKADGDDLVSGEELYDVFLN 120
           EEGDVDKDGLLSMGEFLEMNARSVEVGEVMGSYLKIAYEALKADGDDLVSGEELYDVFLN
Sbjct: 61  EEGDVDKDGLLSMGEFLEMNARSVEVGEVMGSYLKIAYEALKADGDDLVSGEELYDVFLN 120

Query: 121 VGLEVSVEDCNAIVASMDGDEDGAVFLEDLKLIVDSLHPNSNPQL 166
           VGLEVSVEDCNAIVASMDGDEDGAVFLEDLKLIVDSLHPNSNPQL
Sbjct: 121 VGLEVSVEDCNAIVASMDGDEDGAVFLEDLKLIVDSLHPNSNPQL 165

[Close]

BLAST of Moc11g07780 vs. NCBI nr
Match: XP_038897482.1 (probable calcium-binding protein CML29 [Benincasa hispida])

HSP 1 Score: 260.4 bits (664), Expect = 1.1e-65
Identity = 131/158 (82.91%), Postives = 152/158 (96.20%), Query Frame = 0


Query: 1   MAQAGSLSAETESLSHMFSLIEAFRAFDADNDGLITAPEVGGIMGSLGYNLSEEDVNLMM 60
           MAQAGSLSAETE+LSH+F+LIEAFRAFDADNDGLI++ EVGGIMGSLGY+++E+DVN+MM
Sbjct: 1   MAQAGSLSAETETLSHIFNLIEAFRAFDADNDGLISSAEVGGIMGSLGYDMNEQDVNMMM 60

Query: 61  EEGDVDKDGLLSMGEFLEMNARSVEVGEVMGSYLKIAYEALKADGDDLVSGEELYDVFLN 120
           EEGDVD+DGLLS+GEFLEMNA+++EVGE +GSYLKIA+EALKADGDDLVSGEELYDVF+N
Sbjct: 61  EEGDVDRDGLLSIGEFLEMNAKNMEVGE-LGSYLKIAFEALKADGDDLVSGEELYDVFVN 120

Query: 121 VGLEVSVEDCNAIVASMDGDEDGAVFLEDLKLIVDSLH 159
           +GLEVS+EDC AIVASMDGD DGAVFL D KLIV+SLH
Sbjct: 121 LGLEVSLEDCMAIVASMDGDGDGAVFLHDFKLIVNSLH 157

[Close]

BLAST of Moc11g07780 vs. NCBI nr
Match: XP_022981068.1 (probable calcium-binding protein CML29 [Cucurbita maxima])

HSP 1 Score: 259.2 bits (661), Expect = 2.4e-65
Identity = 134/157 (85.35%), Postives = 149/157 (94.90%), Query Frame = 0


Query: 1   MAQAGSLSAETESLSHMFSLIEAFRAFDADNDGLITAPEVGGIMGSLGYNLSEEDVNLMM 60
           MAQ  SLSAET+SLSH+FSLIEAFRAFDADNDGLI+A EVGGIMGSLGYNLSE+DVN+MM
Sbjct: 1   MAQEASLSAETDSLSHIFSLIEAFRAFDADNDGLISAAEVGGIMGSLGYNLSEQDVNVMM 60

Query: 61  EEGDVDKDGLLSMGEFLEMNARSVEVGEVMGSYLKIAYEALKADGDDLVSGEELYDVFLN 120
           EEGDVD+DGLLSMGEFLEM+ARSVEVGEV G YLK+A+EAL ADGDDLVSGEELY+VF+N
Sbjct: 61  EEGDVDRDGLLSMGEFLEMSARSVEVGEV-GRYLKVAFEALNADGDDLVSGEELYEVFVN 120

Query: 121 VGLEVSVEDCNAIVASMDGDEDGAVFLEDLKLIVDSL 158
           +GL+VS+EDC AIVASMDGD DGAVFLEDLKLIV+SL
Sbjct: 121 LGLDVSLEDCMAIVASMDGDGDGAVFLEDLKLIVNSL 156

[Close]

BLAST of Moc11g07780 vs. NCBI nr
Match: XP_022941005.1 (probable calcium-binding protein CML29 [Cucurbita moschata])

HSP 1 Score: 258.5 bits (659), Expect = 4.0e-65
Identity = 134/157 (85.35%), Postives = 149/157 (94.90%), Query Frame = 0


Query: 1   MAQAGSLSAETESLSHMFSLIEAFRAFDADNDGLITAPEVGGIMGSLGYNLSEEDVNLMM 60
           MAQ  SLSAET+SLSH+FSLIEAFRAFDADNDGLI+A EVGGIMGSLGYNLSE+DVN+MM
Sbjct: 1   MAQQPSLSAETDSLSHIFSLIEAFRAFDADNDGLISAAEVGGIMGSLGYNLSEQDVNVMM 60

Query: 61  EEGDVDKDGLLSMGEFLEMNARSVEVGEVMGSYLKIAYEALKADGDDLVSGEELYDVFLN 120
           EEGDVD+DGLLSMGEFLEM+ARSVEVGEV G YLK+A+EAL ADGDDLVSGEELY+VF+N
Sbjct: 61  EEGDVDRDGLLSMGEFLEMSARSVEVGEV-GRYLKVAFEALNADGDDLVSGEELYEVFVN 120

Query: 121 VGLEVSVEDCNAIVASMDGDEDGAVFLEDLKLIVDSL 158
           +GL+VS+EDC AIVASMDGD DGAVFLEDLKLIV+SL
Sbjct: 121 LGLDVSLEDCMAIVASMDGDGDGAVFLEDLKLIVNSL 156

[Close]

BLAST of Moc11g07780 vs. NCBI nr
Match: KAG6608067.1 (putative calcium-binding protein CML29, partial [Cucurbita argyrosperma subsp. sororia] >KAG7031697.1 putative calcium-binding protein CML29, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 255.8 bits (652), Expect = 2.6e-64
Identity = 132/157 (84.08%), Postives = 148/157 (94.27%), Query Frame = 0


Query: 1   MAQAGSLSAETESLSHMFSLIEAFRAFDADNDGLITAPEVGGIMGSLGYNLSEEDVNLMM 60
           MAQ  SLSA+T+SLSH+FSLIEAFRAFDADNDGLI+A EVGGIMGSLGYNLSE+DVN+MM
Sbjct: 1   MAQQPSLSAQTDSLSHIFSLIEAFRAFDADNDGLISAAEVGGIMGSLGYNLSEQDVNMMM 60

Query: 61  EEGDVDKDGLLSMGEFLEMNARSVEVGEVMGSYLKIAYEALKADGDDLVSGEELYDVFLN 120
           EEGDVD+DGLLSMGEFLEM+ RSVEVGEV G YLK+A+EAL ADGDDLVSGEELY+VF+N
Sbjct: 61  EEGDVDQDGLLSMGEFLEMSGRSVEVGEV-GRYLKVAFEALNADGDDLVSGEELYEVFVN 120

Query: 121 VGLEVSVEDCNAIVASMDGDEDGAVFLEDLKLIVDSL 158
           +GL+VS+EDC AIVASMDGD DGAVFLEDLKLIV+SL
Sbjct: 121 LGLDVSLEDCMAIVASMDGDGDGAVFLEDLKLIVNSL 156

[Close]

BLAST of Moc11g07780 vs. ExPASy Swiss-Prot
Match: Q5Z676 (Probable calcium-binding protein CML29 OS=Oryza sativa subsp. japonica OX=39947 GN=CML29 PE=2 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 2.3e-26
Identity = 72/157 (45.86%), Postives = 103/157 (65.61%), Query Frame = 0


Query: 3   QAGSLSAETESLSHMFSLIEAFRAFDADNDGLITAPEVGGIMGSLGYNLSEEDVNLMMEE 62
           QA  LS + E+LS++ SL+EAF+AFD+DNDGL+TAPE+ G++ SLG +  E +V  M+  
Sbjct: 15  QAQQLSVDFEALSYISSLVEAFQAFDSDNDGLVTAPELRGLLASLGLDKPEHEVRDMLAR 74

Query: 63  GDVDKDGLLSMGEFLE-MNARSVEVGEVMGSYLKIAYEALK-ADGDDLVSGEELYDVFLN 122
            D D+DG LS+ E L+ MNA  + +G  +G+ L+ A  AL+ A G D V G +     L+
Sbjct: 75  ADADRDGKLSVEELLDVMNAGQLGLG-ALGALLQSAVPALESAAGPDGVLGADELARLLS 134

Query: 123 VGLEVSVEDCNAIVASMDGDEDGAVFLEDLKLIVDSL 158
           V    SVEDC  I+A MDGD DGA+ +E+ +L+   L
Sbjct: 135 VMGTASVEDCMEIIACMDGDGDGAISVEEFRLMAQLL 170

[Close]

BLAST of Moc11g07780 vs. ExPASy Swiss-Prot
Match: Q9FYK2 (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 PE=2 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 8.6e-18
Identity = 44/131 (33.59%), Postives = 81/131 (61.83%), Query Frame = 0


Query: 24  FRAFDADNDGLITAPEVGGIMGSLGYNLSEEDVNLMMEEGDVDKDGLLSMGEFLEMNARS 83
           F+ FD + DG I++ E+G IM SLG+ + EE++   + E D   DG ++  EF+E+N + 
Sbjct: 42  FKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEEFVELNTKG 101

Query: 84  VEVGEVMGSYLKIAYEALKADGDDLVSGEELYDVFLNVGLEVSVEDCNAIVASMDGDEDG 143
           ++  +V+ + LK A+     DG+  +S EEL++V  ++G E S+ +C  ++  +D D DG
Sbjct: 102 MDQNDVLEN-LKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIGGVDKDGDG 161

Query: 144 AVFLEDLKLIV 155
            +  E+ K+++
Sbjct: 162 TIDFEEFKIMM 171

[Close]

BLAST of Moc11g07780 vs. ExPASy Swiss-Prot
Match: Q9LE22 (Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 PE=1 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 3.6e-16
Identity = 48/153 (31.37%), Postives = 85/153 (55.56%), Query Frame = 0


Query: 11  TESLSHMFSLIEAFRAFDADNDGLITAPEVGGIMGSLGYNLSEEDVNLMMEEGDVDKDGL 70
           T  +++   L + F  FD++ DG I+  E+GG+  ++G + +E ++N ++EE D D+DG 
Sbjct: 15  TVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGY 74

Query: 71  LSMGEFLEMNARSVEVGEVMGSYLKIAYEALKADGDDLVSGEELYDVFLNVGLEVSVEDC 130
           +++ EF  +   S    E+     + A++    D + L+S  EL+ V   +G+  SVEDC
Sbjct: 75  INLDEFSTLCRSSSSAAEI-----RDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDC 134

Query: 131 NAIVASMDGDEDGAVFLEDL-KLIVDSLHPNSN 163
             ++  +D D DG V  E+  K++  S   NSN
Sbjct: 135 TRMIGPVDADGDGNVNFEEFQKMMTSSSLLNSN 162

[Close]

BLAST of Moc11g07780 vs. ExPASy Swiss-Prot
Match: Q8RZB5 (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 GN=CML10 PE=2 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 8.0e-16
Identity = 42/131 (32.06%), Postives = 74/131 (56.49%), Query Frame = 0


Query: 24  FRAFDADNDGLITAPEVGGIMGSLGYNLSEEDVNLMMEEGDVDKDGLLSMGEFLEMNARS 83
           FR FDA+ DG I+  E+G +  SLG+  +++++  MM E D D DG +S+ EF  +NA +
Sbjct: 45  FRKFDANGDGRISRSELGALFESLGHAATDDELARMMAEADADGDGFISLDEFAALNATA 104

Query: 84  VEVGEVMGSYLKIAYEALKADGDDLVSGEELYDVFLNVGLEVSVEDCNAIVASMDGDEDG 143
                 +   L+ A+    ADG+  +S  EL  V   +G + +V+ C  ++  +D + DG
Sbjct: 105 SGDAAAVEEDLRHAFRVFDADGNGTISAAELARVLHGLGEKATVQQCRRMIEGVDQNGDG 164

Query: 144 AVFLEDLKLIV 155
            +  E+ K+++
Sbjct: 165 LISFEEFKVMM 175

[Close]

BLAST of Moc11g07780 vs. ExPASy Swiss-Prot
Match: Q9M7R0 (Calcium-binding allergen Ole e 8 OS=Olea europaea OX=4146 PE=1 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 2.3e-15
Identity = 46/141 (32.62%), Postives = 77/141 (54.61%), Query Frame = 0


Query: 24  FRAFDADNDGLITAPEVGGIMGSLGYNLSEEDVNLMMEEGDVDKDGLLSMGEFLEMNARS 83
           F  FDA+ DG I+  E+ G++ +LG N S+E++  +MEE D DKDG +++ EF       
Sbjct: 25  FNRFDANGDGKISGDELAGVLKALGSNTSKEEIGRIMEEIDTDKDGFINVQEFAAFVKAE 84

Query: 84  VEVGEVMG--SYLKIAYEALKADGDDLVSGEELYDVFLNVGLEVSVEDCNAIVASMDGDE 143
            +     G  + LK A+E    D + L+S  EL+ +   +G   +  DC  ++ S+D D 
Sbjct: 85  TDPYPSSGGENELKEAFELYDQDHNGLISSVELHKILTRLGERYAEHDCVEMIKSVDSDG 144

Query: 144 DGAVFLEDLKLIVDSLHPNSN 163
           DG V  E+ K ++ +   N++
Sbjct: 145 DGYVSFEEFKKMMTNKSGNNS 165

[Close]

BLAST of Moc11g07780 vs. ExPASy TrEMBL
Match: A0A6J1CMA3 (probable calcium-binding protein CML29 OS=Momordica charantia OX=3673 GN=LOC111012911 PE=4 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 4.2e-84
Identity = 165/165 (100.00%), Postives = 165/165 (100.00%), Query Frame = 0


Query: 1   MAQAGSLSAETESLSHMFSLIEAFRAFDADNDGLITAPEVGGIMGSLGYNLSEEDVNLMM 60
           MAQAGSLSAETESLSHMFSLIEAFRAFDADNDGLITAPEVGGIMGSLGYNLSEEDVNLMM
Sbjct: 1   MAQAGSLSAETESLSHMFSLIEAFRAFDADNDGLITAPEVGGIMGSLGYNLSEEDVNLMM 60

Query: 61  EEGDVDKDGLLSMGEFLEMNARSVEVGEVMGSYLKIAYEALKADGDDLVSGEELYDVFLN 120
           EEGDVDKDGLLSMGEFLEMNARSVEVGEVMGSYLKIAYEALKADGDDLVSGEELYDVFLN
Sbjct: 61  EEGDVDKDGLLSMGEFLEMNARSVEVGEVMGSYLKIAYEALKADGDDLVSGEELYDVFLN 120

Query: 121 VGLEVSVEDCNAIVASMDGDEDGAVFLEDLKLIVDSLHPNSNPQL 166
           VGLEVSVEDCNAIVASMDGDEDGAVFLEDLKLIVDSLHPNSNPQL
Sbjct: 121 VGLEVSVEDCNAIVASMDGDEDGAVFLEDLKLIVDSLHPNSNPQL 165

[Close]

BLAST of Moc11g07780 vs. ExPASy TrEMBL
Match: A0A6J1J135 (probable calcium-binding protein CML29 OS=Cucurbita maxima OX=3661 GN=LOC111480326 PE=4 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 1.1e-65
Identity = 134/157 (85.35%), Postives = 149/157 (94.90%), Query Frame = 0


Query: 1   MAQAGSLSAETESLSHMFSLIEAFRAFDADNDGLITAPEVGGIMGSLGYNLSEEDVNLMM 60
           MAQ  SLSAET+SLSH+FSLIEAFRAFDADNDGLI+A EVGGIMGSLGYNLSE+DVN+MM
Sbjct: 1   MAQEASLSAETDSLSHIFSLIEAFRAFDADNDGLISAAEVGGIMGSLGYNLSEQDVNVMM 60

Query: 61  EEGDVDKDGLLSMGEFLEMNARSVEVGEVMGSYLKIAYEALKADGDDLVSGEELYDVFLN 120
           EEGDVD+DGLLSMGEFLEM+ARSVEVGEV G YLK+A+EAL ADGDDLVSGEELY+VF+N
Sbjct: 61  EEGDVDRDGLLSMGEFLEMSARSVEVGEV-GRYLKVAFEALNADGDDLVSGEELYEVFVN 120

Query: 121 VGLEVSVEDCNAIVASMDGDEDGAVFLEDLKLIVDSL 158
           +GL+VS+EDC AIVASMDGD DGAVFLEDLKLIV+SL
Sbjct: 121 LGLDVSLEDCMAIVASMDGDGDGAVFLEDLKLIVNSL 156

[Close]

BLAST of Moc11g07780 vs. ExPASy TrEMBL
Match: A0A6J1FSB7 (probable calcium-binding protein CML29 OS=Cucurbita moschata OX=3662 GN=LOC111446418 PE=4 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 2.0e-65
Identity = 134/157 (85.35%), Postives = 149/157 (94.90%), Query Frame = 0


Query: 1   MAQAGSLSAETESLSHMFSLIEAFRAFDADNDGLITAPEVGGIMGSLGYNLSEEDVNLMM 60
           MAQ  SLSAET+SLSH+FSLIEAFRAFDADNDGLI+A EVGGIMGSLGYNLSE+DVN+MM
Sbjct: 1   MAQQPSLSAETDSLSHIFSLIEAFRAFDADNDGLISAAEVGGIMGSLGYNLSEQDVNVMM 60

Query: 61  EEGDVDKDGLLSMGEFLEMNARSVEVGEVMGSYLKIAYEALKADGDDLVSGEELYDVFLN 120
           EEGDVD+DGLLSMGEFLEM+ARSVEVGEV G YLK+A+EAL ADGDDLVSGEELY+VF+N
Sbjct: 61  EEGDVDRDGLLSMGEFLEMSARSVEVGEV-GRYLKVAFEALNADGDDLVSGEELYEVFVN 120

Query: 121 VGLEVSVEDCNAIVASMDGDEDGAVFLEDLKLIVDSL 158
           +GL+VS+EDC AIVASMDGD DGAVFLEDLKLIV+SL
Sbjct: 121 LGLDVSLEDCMAIVASMDGDGDGAVFLEDLKLIVNSL 156

[Close]

BLAST of Moc11g07780 vs. ExPASy TrEMBL
Match: A0A1S3B9P7 (probable calcium-binding protein CML29 OS=Cucumis melo OX=3656 GN=LOC103487564 PE=4 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 1.6e-62
Identity = 127/158 (80.38%), Postives = 146/158 (92.41%), Query Frame = 0


Query: 1   MAQAGSLSAETESLSHMFSLIEAFRAFDADNDGLITAPEVGGIMGSLGYNLSEEDVNLMM 60
           MAQAGSL  +TE+LSH+F+LIEAFRAFDADNDGLI++ EVGGI+GSLGYNLSEEDV +MM
Sbjct: 1   MAQAGSLLVDTEALSHIFNLIEAFRAFDADNDGLISSAEVGGIVGSLGYNLSEEDVKMMM 60

Query: 61  EEGDVDKDGLLSMGEFLEMNARSVEVGEVMGSYLKIAYEALKADGDDLVSGEELYDVFLN 120
           EEGD DKDGLLSMGEFLEMNA+++EVGE +GSYLKIA EALKADGDDLVSGEELYDVF+N
Sbjct: 61  EEGDADKDGLLSMGEFLEMNAKNMEVGE-LGSYLKIALEALKADGDDLVSGEELYDVFVN 120

Query: 121 VGLEVSVEDCNAIVASMDGDEDGAVFLEDLKLIVDSLH 159
           +GL+VS+ED  AIVAS+DGD DGA+FL D KLIV+SLH
Sbjct: 121 LGLDVSLEDSMAIVASLDGDGDGAMFLHDFKLIVNSLH 157

[Close]

BLAST of Moc11g07780 vs. ExPASy TrEMBL
Match: A0A5D3D6S0 (Putative calcium-binding protein CML29 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1493G00180 PE=4 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 1.6e-62
Identity = 127/158 (80.38%), Postives = 146/158 (92.41%), Query Frame = 0


Query: 1   MAQAGSLSAETESLSHMFSLIEAFRAFDADNDGLITAPEVGGIMGSLGYNLSEEDVNLMM 60
           MAQAGSL  +TE+LSH+F+LIEAFRAFDADNDGLI++ EVGGI+GSLGYNLSEEDV +MM
Sbjct: 1   MAQAGSLLVDTEALSHIFNLIEAFRAFDADNDGLISSAEVGGIVGSLGYNLSEEDVKMMM 60

Query: 61  EEGDVDKDGLLSMGEFLEMNARSVEVGEVMGSYLKIAYEALKADGDDLVSGEELYDVFLN 120
           EEGD DKDGLLSMGEFLEMNA+++EVGE +GSYLKIA EALKADGDDLVSGEELYDVF+N
Sbjct: 61  EEGDADKDGLLSMGEFLEMNAKNMEVGE-LGSYLKIALEALKADGDDLVSGEELYDVFVN 120

Query: 121 VGLEVSVEDCNAIVASMDGDEDGAVFLEDLKLIVDSLH 159
           +GL+VS+ED  AIVAS+DGD DGA+FL D KLIV+SLH
Sbjct: 121 LGLDVSLEDSMAIVASLDGDGDGAMFLHDFKLIVNSLH 157

[Close]

BLAST of Moc11g07780 vs. TAIR 10
Match: AT1G24620.1 (EF hand calcium-binding protein family )

HSP 1 Score: 91.7 bits (226), Expect = 6.1e-19
Identity = 44/131 (33.59%), Postives = 81/131 (61.83%), Query Frame = 0


Query: 24  FRAFDADNDGLITAPEVGGIMGSLGYNLSEEDVNLMMEEGDVDKDGLLSMGEFLEMNARS 83
           F+ FD + DG I++ E+G IM SLG+ + EE++   + E D   DG ++  EF+E+N + 
Sbjct: 42  FKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFEEFVELNTKG 101

Query: 84  VEVGEVMGSYLKIAYEALKADGDDLVSGEELYDVFLNVGLEVSVEDCNAIVASMDGDEDG 143
           ++  +V+ + LK A+     DG+  +S EEL++V  ++G E S+ +C  ++  +D D DG
Sbjct: 102 MDQNDVLEN-LKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIGGVDKDGDG 161

Query: 144 AVFLEDLKLIV 155
            +  E+ K+++
Sbjct: 162 TIDFEEFKIMM 171

[Close]

BLAST of Moc11g07780 vs. TAIR 10
Match: AT1G18210.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 86.3 bits (212), Expect = 2.6e-17
Identity = 48/153 (31.37%), Postives = 85/153 (55.56%), Query Frame = 0


Query: 11  TESLSHMFSLIEAFRAFDADNDGLITAPEVGGIMGSLGYNLSEEDVNLMMEEGDVDKDGL 70
           T  +++   L + F  FD++ DG I+  E+GG+  ++G + +E ++N ++EE D D+DG 
Sbjct: 15  TVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGY 74

Query: 71  LSMGEFLEMNARSVEVGEVMGSYLKIAYEALKADGDDLVSGEELYDVFLNVGLEVSVEDC 130
           +++ EF  +   S    E+     + A++    D + L+S  EL+ V   +G+  SVEDC
Sbjct: 75  INLDEFSTLCRSSSSAAEI-----RDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDC 134

Query: 131 NAIVASMDGDEDGAVFLEDL-KLIVDSLHPNSN 163
             ++  +D D DG V  E+  K++  S   NSN
Sbjct: 135 TRMIGPVDADGDGNVNFEEFQKMMTSSSLLNSN 162

[Close]

BLAST of Moc11g07780 vs. TAIR 10
Match: AT1G18210.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 86.3 bits (212), Expect = 2.6e-17
Identity = 48/153 (31.37%), Postives = 85/153 (55.56%), Query Frame = 0


Query: 11  TESLSHMFSLIEAFRAFDADNDGLITAPEVGGIMGSLGYNLSEEDVNLMMEEGDVDKDGL 70
           T  +++   L + F  FD++ DG I+  E+GG+  ++G + +E ++N ++EE D D+DG 
Sbjct: 15  TVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGY 74

Query: 71  LSMGEFLEMNARSVEVGEVMGSYLKIAYEALKADGDDLVSGEELYDVFLNVGLEVSVEDC 130
           +++ EF  +   S    E+     + A++    D + L+S  EL+ V   +G+  SVEDC
Sbjct: 75  INLDEFSTLCRSSSSAAEI-----RDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDC 134

Query: 131 NAIVASMDGDEDGAVFLEDL-KLIVDSLHPNSN 163
             ++  +D D DG V  E+  K++  S   NSN
Sbjct: 135 TRMIGPVDADGDGNVNFEEFQKMMTSSSLLNSN 162

[Close]

BLAST of Moc11g07780 vs. TAIR 10
Match: AT1G73630.1 (EF hand calcium-binding protein family )

HSP 1 Score: 77.8 bits (190), Expect = 9.1e-15
Identity = 42/137 (30.66%), Postives = 77/137 (56.20%), Query Frame = 0


Query: 20  LIEAFRAFDADNDGLITAPEVGGIMGSLGYNLSEEDVNLMMEEGDVDKDGLLSMGEFLEM 79
           L + F  FDA+ DG I+  E+G +  S+G + +EE++N +++E D+D DG ++  EF  +
Sbjct: 21  LKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLDEIDIDCDGFINQEEFATI 80

Query: 80  NARSVEVGEVMGSYLKIAYEALKADGDDLVSGEELYDVFLNVGLEVSVEDCNAIVASMDG 139
              S    E+     + A++    + + L+S  E++ V   +G+  SVEDC  ++  +D 
Sbjct: 81  CRSSSSAVEI-----REAFDLYDQNKNGLISSSEIHKVLNRLGMTCSVEDCVRMIGHVDT 140

Query: 140 DEDGAVFLEDLKLIVDS 157
           D DG V  E+ + ++ S
Sbjct: 141 DGDGNVNFEEFQKMMSS 152

[Close]

BLAST of Moc11g07780 vs. TAIR 10
Match: AT3G10190.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 77.4 bits (189), Expect = 1.2e-14
Identity = 49/153 (32.03%), Postives = 85/153 (55.56%), Query Frame = 0


Query: 6   SLSAETESLSHMFSLIEAFRAFDADNDGLITAPEVGGIMGSLGYN-LSEEDVNLMMEEGD 65
           S+  E  S      +++AF+  D DNDG ++  ++  ++  LG + L+EE++N+M++E D
Sbjct: 57  SILPEVPSPYSYVEILQAFKLIDRDNDGAVSRHDLESLLSRLGPDPLTEEEINVMLKEVD 116

Query: 66  VDKDGLLSMGEFLEMNARSVEVGEVMGS-YLKIAYEALKADGDDLVSGEELYDVFLNVGL 125
            D DG + +    E+ +R V +     S  LK  +E   AD D L+S +EL  VF  +G 
Sbjct: 117 CDGDGTIRLE---ELASRVVSLDPARDSTELKETFEFFDADRDGLISADELLRVFSTIGD 176

Query: 126 E-VSVEDCNAIVASMDGDEDGAVFLEDLKLIVD 156
           E  +++DC  ++A +D D DG V   +   ++D
Sbjct: 177 ERCTLDDCKRMIADVDEDGDGFVCFTEFSRMMD 206

The following BLAST results are available for this feature:

BLAST of Moc11g07780 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022142920.1	8.7e-84	100.00	probable calcium-binding protein CML29 [Momordica charantia]	[more]
XP_038897482.1	1.1e-65	82.91	probable calcium-binding protein CML29 [Benincasa hispida]	[more]
XP_022981068.1	2.4e-65	85.35	probable calcium-binding protein CML29 [Cucurbita maxima]	[more]
XP_022941005.1	4.0e-65	85.35	probable calcium-binding protein CML29 [Cucurbita moschata]	[more]
KAG6608067.1	2.6e-64	84.08	putative calcium-binding protein CML29, partial [Cucurbita argyrosperma subsp. s...	[more]

BLAST of Moc11g07780 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5Z676	2.3e-26	45.86	Probable calcium-binding protein CML29 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9FYK2	8.6e-18	33.59	Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 ...	[more]
Q9LE22	3.6e-16	31.37	Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 ...	[more]
Q8RZB5	8.0e-16	32.06	Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9M7R0	2.3e-15	32.62	Calcium-binding allergen Ole e 8 OS=Olea europaea OX=4146 PE=1 SV=1	[more]

BLAST of Moc11g07780 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CMA3	4.2e-84	100.00	probable calcium-binding protein CML29 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1J135	1.1e-65	85.35	probable calcium-binding protein CML29 OS=Cucurbita maxima OX=3661 GN=LOC1114803...	[more]
A0A6J1FSB7	2.0e-65	85.35	probable calcium-binding protein CML29 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A1S3B9P7	1.6e-62	80.38	probable calcium-binding protein CML29 OS=Cucumis melo OX=3656 GN=LOC103487564 P...	[more]
A0A5D3D6S0	1.6e-62	80.38	Putative calcium-binding protein CML29 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]

BLAST of Moc11g07780 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G24620.1	6.1e-19	33.59	EF hand calcium-binding protein family	[more]
AT1G18210.1	2.6e-17	31.37	Calcium-binding EF-hand family protein	[more]
AT1G18210.2	2.6e-17	31.37	Calcium-binding EF-hand family protein	[more]
AT1G73630.1	9.1e-15	30.66	EF hand calcium-binding protein family	[more]
AT3G10190.1	1.2e-14	32.03	Calcium-binding EF-hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 55..83 e-value: 2.3 score: 14.8 coord: 129..157 e-value: 130.0 score: 0.7 coord: 19..47 e-value: 0.0056 score: 25.9
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 20..79 e-value: 3.5E-9 score: 37.0
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 15..50 score: 12.225983
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 51..86 score: 8.794848
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 20..79 e-value: 1.14136E-15 score: 65.6469
None	No IPR available	GENE3D	1.10.238.10		coord: 3..88 e-value: 3.9E-18 score: 67.8
None	No IPR available	GENE3D	1.10.238.10		coord: 92..156 e-value: 7.8E-6 score: 27.9
None	No IPR available	PANTHER	PTHR23050:SF382	CALCIUM-BINDING PROTEIN CML25-RELATED	coord: 14..157
None	No IPR available	PANTHER	PTHR23050	CALCIUM BINDING PROTEIN	coord: 14..157
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 64..76
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 28..40
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 15..157

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc11g07780.1	Moc11g07780.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding