Moc11g07140 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc11g07140
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	hydroxyproline-rich glycoprotein family protein
Location	chr11: 4987197 .. 4988158 (+)
RNA-Seq Expression	Moc11g07140
Synteny	Moc11g07140

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTACGATGATCGCGAAGCACAGGATCCGGCCTCCCTAAGCTTTCCCGTCGGTTTGGTTCTTCTGCTCACATTTTTTTTCTGCATGTGTTGCTTTTTTTCTTGTTGCCTTCACTGGGATAAGCTCCGATCATTCCTCGGCTGTCCCGACCACCGCCATCCACCTCCTATTCCGCCGGAAAACGCCGCCTCATCGCCGCCAGACAAGATTCTGCCTCTCCATACGGTGCGTTTTGATTCTTCTTTTTTTTTATCTTGATTGTAGAAGCTACTCTACAAGTTTCCATTTCAATTTGATTTAATATTTTTTTATTAGATCTCCAATACTAGCAAATAAATAGACACTAAACATATAAAAATGCCTAATTTTCTCCCCAAATTTGACAAAACTTTTGTTTTTTTTTTTTAATGTCTCCGGCCTAATTATTTATTATCTACCAACTAAATGTGAAAATTGGTATCACATGTTAATGAGTTTATTTTAACATCACTATGAAAATAGTATAATTAATTATATTAAAATTTAAATGACTAGATTGAAACCTTTTAGTCATACGAATTGAATTGAAAAGAAAAATACCTAAAAACATTATTAAAATTTAAAACTATATTTAATTTATTGATATCAGTGCGGTTTGCTAACTTTTTATCTTTTTTTTTTGTTGATATAAGTTTTTTATTATTATTATTGTTATGAGTTTTTTTTATTGATATATTTAACTTTTTTTTGTTGTACTGAAAGAAAAACTTTTGATCCTCTTTTTTTCCTCAACGATTAAATTGTGGATGCAGAACCAGTCGCAGAGCCTGCCGGTGGTGATGCCCGGCGACGAGTTTCCCAGGTTCATAGCAATGGCTTGCCCTCCGTGTGCGGCGGCGGTTGCGGCGGCTCCGTTTGTGGATGTTTTAGTGCAGAAACCTCCACAAAGTACTCCGGATTCTTCTGGAGCTCTCTCTTAA

mRNA sequence

ATGGATTACGATGATCGCGAAGCACAGGATCCGGCCTCCCTAAGCTTTCCCGTCGGTTTGGTTCTTCTGCTCACATTTTTTTTCTGCATGTGTTGCTTTTTTTCTTGTTGCCTTCACTGGGATAAGCTCCGATCATTCCTCGGCTGTCCCGACCACCGCCATCCACCTCCTATTCCGCCGGAAAACGCCGCCTCATCGCCGCCAGACAAGATTCTGCCTCTCCATACGAACCAGTCGCAGAGCCTGCCGGTGGTGATGCCCGGCGACGAGTTTCCCAGGTTCATAGCAATGGCTTGCCCTCCGTGTGCGGCGGCGGTTGCGGCGGCTCCGTTTGTGGATGTTTTAGTGCAGAAACCTCCACAAAGTACTCCGGATTCTTCTGGAGCTCTCTCTTAA

Coding sequence (CDS)

ATGGATTACGATGATCGCGAAGCACAGGATCCGGCCTCCCTAAGCTTTCCCGTCGGTTTGGTTCTTCTGCTCACATTTTTTTTCTGCATGTGTTGCTTTTTTTCTTGTTGCCTTCACTGGGATAAGCTCCGATCATTCCTCGGCTGTCCCGACCACCGCCATCCACCTCCTATTCCGCCGGAAAACGCCGCCTCATCGCCGCCAGACAAGATTCTGCCTCTCCATACGAACCAGTCGCAGAGCCTGCCGGTGGTGATGCCCGGCGACGAGTTTCCCAGGTTCATAGCAATGGCTTGCCCTCCGTGTGCGGCGGCGGTTGCGGCGGCTCCGTTTGTGGATGTTTTAGTGCAGAAACCTCCACAAAGTACTCCGGATTCTTCTGGAGCTCTCTCTTAA

Protein sequence

MDYDDREAQDPASLSFPVGLVLLLTFFFCMCCFFSCCLHWDKLRSFLGCPDHRHPPPIPPENAASSPPDKILPLHTNQSQSLPVVMPGDEFPRFIAMACPPCAAAVAAAPFVDVLVQKPPQSTPDSSGALS

Homology

[Close]

BLAST of Moc11g07140 vs. NCBI nr
Match: XP_022140744.1 (uncharacterized protein At5g65660-like [Momordica charantia])

HSP 1 Score: 274.6 bits (701), Expect = 4.3e-70
Identity = 131/131 (100.00%), Postives = 131/131 (100.00%), Query Frame = 0


Query: 1   MDYDDREAQDPASLSFPVGLVLLLTFFFCMCCFFSCCLHWDKLRSFLGCPDHRHPPPIPP 60
           MDYDDREAQDPASLSFPVGLVLLLTFFFCMCCFFSCCLHWDKLRSFLGCPDHRHPPPIPP
Sbjct: 1   MDYDDREAQDPASLSFPVGLVLLLTFFFCMCCFFSCCLHWDKLRSFLGCPDHRHPPPIPP 60

Query: 61  ENAASSPPDKILPLHTNQSQSLPVVMPGDEFPRFIAMACPPCAAAVAAAPFVDVLVQKPP 120
           ENAASSPPDKILPLHTNQSQSLPVVMPGDEFPRFIAMACPPCAAAVAAAPFVDVLVQKPP
Sbjct: 61  ENAASSPPDKILPLHTNQSQSLPVVMPGDEFPRFIAMACPPCAAAVAAAPFVDVLVQKPP 120

Query: 121 QSTPDSSGALS 132
           QSTPDSSGALS
Sbjct: 121 QSTPDSSGALS 131

[Close]

BLAST of Moc11g07140 vs. NCBI nr
Match: XP_038899315.1 (uncharacterized protein At5g65660-like [Benincasa hispida])

HSP 1 Score: 199.5 bits (506), Expect = 1.8e-47
Identity = 100/134 (74.63%), Postives = 110/134 (82.09%), Query Frame = 0


Query: 1   MDYDDREAQDPASLSFPVGLVLLLTFFFCMCCFFSCCLHWDKLRSFLGCPD--HRHPPPI 60
           M+YD+REAQDPASLSFPVGLVLLLTF FCMCCFFSCCLHW+KLRSFLGCPD  H H PPI
Sbjct: 1   MEYDNREAQDPASLSFPVGLVLLLTFLFCMCCFFSCCLHWEKLRSFLGCPDHLHHHHPPI 60

Query: 61  PPENAASSPPDKILPLHT-----NQSQSLPVVMPGDEFPRFIAMACPPCAAAVAAAPFVD 120
           PP+ AA SPPDKI P+HT     N+ QSL V+MPGDE PRFIAMACPPCAAA      V+
Sbjct: 61  PPQTAAFSPPDKISPIHTKIWRENRPQSLSVLMPGDEVPRFIAMACPPCAAAA----LVE 120

Query: 121 VLVQKPPQSTPDSS 128
           ++VQKP QS PDSS
Sbjct: 121 IVVQKPSQSFPDSS 130

[Close]

BLAST of Moc11g07140 vs. NCBI nr
Match: XP_008466113.1 (PREDICTED: uncharacterized protein At5g65660-like [Cucumis melo] >KAA0038642.1 uncharacterized protein E6C27_scaffold92G001760 [Cucumis melo var. makuwa] >TYK31243.1 uncharacterized protein E5676_scaffold455G005320 [Cucumis melo var. makuwa])

HSP 1 Score: 187.6 bits (475), Expect = 7.0e-44
Identity = 96/135 (71.11%), Postives = 108/135 (80.00%), Query Frame = 0


Query: 1   MDYDDREAQDPASLSFPVGLVLLLTFFFCMCCFFSCCLHWDKLRSFLGCPD--HRHPPPI 60
           M+YD+REAQDPASLSFPVGLVLLLTF FCMCCFF CCLHW+KLRSFLGCPD  H H PPI
Sbjct: 1   MEYDNREAQDPASLSFPVGLVLLLTFLFCMCCFFCCCLHWEKLRSFLGCPDHLHHHHPPI 60

Query: 61  PPEN--AASSPPDKILPLHT----NQSQSLPVVMPGDEFPRFIAMACPPCAAAVAAAPFV 120
           PP +  AA SPPDK  P+HT    N+ QS+ V+MPGDE PRFIA+ACPPCA A AA   V
Sbjct: 61  PPPHSPAALSPPDKFSPIHTIWKENRPQSVSVLMPGDEVPRFIALACPPCATAAAA--LV 120

Query: 121 DVLVQKPPQSTPDSS 128
           +++VQKP QS  DSS
Sbjct: 121 EIVVQKPSQSISDSS 133

[Close]

BLAST of Moc11g07140 vs. NCBI nr
Match: XP_022936981.1 (uncharacterized protein At5g65660-like [Cucurbita moschata])

HSP 1 Score: 183.0 bits (463), Expect = 1.7e-42
Identity = 96/137 (70.07%), Postives = 106/137 (77.37%), Query Frame = 0


Query: 1   MDYDDREAQDPASLSFPVGLVLLLTFFFCMCCFFSCCLHWDKLRSFLGCP---DHRHPPP 60
           M+YDDREAQD ASLSFPVGLVLLLTF FCMCCFFSCCLHW+KLRS LGCP   DHRH P 
Sbjct: 1   MEYDDREAQDSASLSFPVGLVLLLTFLFCMCCFFSCCLHWEKLRSILGCPDHADHRH-PS 60

Query: 61  IPPENAASSPPDKILPLHT----NQSQSLPVVMPGDEFPRFIAMACPPCAAAVAAAPFVD 120
           IPPE  A SPPDK  P+HT    N+ +SL V+MPGDE PRFIAMACPPC     AAP V+
Sbjct: 61  IPPETPAFSPPDKFSPIHTIWKENRPESLTVLMPGDEVPRFIAMACPPC----GAAPLVE 120

Query: 121 VLVQKPPQSTPDSSGAL 131
           +++QKP QS    SGAL
Sbjct: 121 IVIQKPSQSI---SGAL 129

[Close]

BLAST of Moc11g07140 vs. NCBI nr
Match: KAG6591711.1 (hypothetical protein SDJN03_14057, partial [Cucurbita argyrosperma subsp. sororia] >KAG7024594.1 hypothetical protein SDJN02_13412 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 181.4 bits (459), Expect = 5.0e-42
Identity = 92/133 (69.17%), Postives = 104/133 (78.20%), Query Frame = 0


Query: 1   MDYDDREAQDPASLSFPVGLVLLLTFFFCMCCFFSCCLHWDKLRSFLGCP---DHRHPPP 60
           M+YDDREAQD ASLSFPVGLVLLLTF FCMCCFFSCCLHW+KLRS LGCP   DHRH P 
Sbjct: 1   MEYDDREAQDSASLSFPVGLVLLLTFLFCMCCFFSCCLHWEKLRSILGCPDHADHRH-PS 60

Query: 61  IPPENAASSPPDKILPLHT----NQSQSLPVVMPGDEFPRFIAMACPPCAAAVAAAPFVD 120
           IPPE  A SPPDK  P+HT    N+ +SL V+MPGDE PRFIAMACPPC     AAP V+
Sbjct: 61  IPPETPAFSPPDKFSPIHTIWKENRPESLTVLMPGDEVPRFIAMACPPC----GAAPLVE 120

Query: 121 VLVQKPPQSTPDS 127
           +++QKP QS  ++
Sbjct: 121 IVIQKPSQSISEA 128

[Close]

BLAST of Moc11g07140 vs. ExPASy Swiss-Prot
Match: Q9LSK9 (Uncharacterized protein At5g65660 OS=Arabidopsis thaliana OX=3702 GN=At5g65660 PE=2 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 1.8e-10
Identity = 47/112 (41.96%), Postives = 57/112 (50.89%), Query Frame = 0


Query: 13  SLSFPVGLVLLLTFFFCMCCFFSCCLHWDKLRSFLGCPDHRHPPPIPPENAASSPPDKIL 72
           SL FP+G  LLL   F +   FSCC HWDK RS      +   P    E+    P     
Sbjct: 18  SLGFPLGTALLLIIIFSLSGIFSCCYHWDKHRSLRRSLANGR-PSADIESNPYKPKPPFP 77

Query: 73  PLHTNQSQSLPVVMPGDEFPRFIAMACPPCAAAVAAAPFVDVLVQKPPQSTP 125
            +   Q+ S+PV+MPGD  P+FIA+ C PCA        VD  VQ PPQS P
Sbjct: 78  EMKKPQNLSVPVLMPGDNTPKFIALPC-PCAPPRPEKLTVD--VQTPPQSPP 125

[Close]

BLAST of Moc11g07140 vs. ExPASy TrEMBL
Match: A0A6J1CGJ4 (uncharacterized protein At5g65660-like OS=Momordica charantia OX=3673 GN=LOC111011330 PE=4 SV=1)

HSP 1 Score: 274.6 bits (701), Expect = 2.1e-70
Identity = 131/131 (100.00%), Postives = 131/131 (100.00%), Query Frame = 0


Query: 1   MDYDDREAQDPASLSFPVGLVLLLTFFFCMCCFFSCCLHWDKLRSFLGCPDHRHPPPIPP 60
           MDYDDREAQDPASLSFPVGLVLLLTFFFCMCCFFSCCLHWDKLRSFLGCPDHRHPPPIPP
Sbjct: 1   MDYDDREAQDPASLSFPVGLVLLLTFFFCMCCFFSCCLHWDKLRSFLGCPDHRHPPPIPP 60

Query: 61  ENAASSPPDKILPLHTNQSQSLPVVMPGDEFPRFIAMACPPCAAAVAAAPFVDVLVQKPP 120
           ENAASSPPDKILPLHTNQSQSLPVVMPGDEFPRFIAMACPPCAAAVAAAPFVDVLVQKPP
Sbjct: 61  ENAASSPPDKILPLHTNQSQSLPVVMPGDEFPRFIAMACPPCAAAVAAAPFVDVLVQKPP 120

Query: 121 QSTPDSSGALS 132
           QSTPDSSGALS
Sbjct: 121 QSTPDSSGALS 131

[Close]

BLAST of Moc11g07140 vs. ExPASy TrEMBL
Match: A0A5D3E545 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G005320 PE=4 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 3.4e-44
Identity = 96/135 (71.11%), Postives = 108/135 (80.00%), Query Frame = 0


Query: 1   MDYDDREAQDPASLSFPVGLVLLLTFFFCMCCFFSCCLHWDKLRSFLGCPD--HRHPPPI 60
           M+YD+REAQDPASLSFPVGLVLLLTF FCMCCFF CCLHW+KLRSFLGCPD  H H PPI
Sbjct: 1   MEYDNREAQDPASLSFPVGLVLLLTFLFCMCCFFCCCLHWEKLRSFLGCPDHLHHHHPPI 60

Query: 61  PPEN--AASSPPDKILPLHT----NQSQSLPVVMPGDEFPRFIAMACPPCAAAVAAAPFV 120
           PP +  AA SPPDK  P+HT    N+ QS+ V+MPGDE PRFIA+ACPPCA A AA   V
Sbjct: 61  PPPHSPAALSPPDKFSPIHTIWKENRPQSVSVLMPGDEVPRFIALACPPCATAAAA--LV 120

Query: 121 DVLVQKPPQSTPDSS 128
           +++VQKP QS  DSS
Sbjct: 121 EIVVQKPSQSISDSS 133

[Close]

BLAST of Moc11g07140 vs. ExPASy TrEMBL
Match: A0A1S3CQS4 (uncharacterized protein At5g65660-like OS=Cucumis melo OX=3656 GN=LOC103503633 PE=4 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 3.4e-44
Identity = 96/135 (71.11%), Postives = 108/135 (80.00%), Query Frame = 0


Query: 1   MDYDDREAQDPASLSFPVGLVLLLTFFFCMCCFFSCCLHWDKLRSFLGCPD--HRHPPPI 60
           M+YD+REAQDPASLSFPVGLVLLLTF FCMCCFF CCLHW+KLRSFLGCPD  H H PPI
Sbjct: 1   MEYDNREAQDPASLSFPVGLVLLLTFLFCMCCFFCCCLHWEKLRSFLGCPDHLHHHHPPI 60

Query: 61  PPEN--AASSPPDKILPLHT----NQSQSLPVVMPGDEFPRFIAMACPPCAAAVAAAPFV 120
           PP +  AA SPPDK  P+HT    N+ QS+ V+MPGDE PRFIA+ACPPCA A AA   V
Sbjct: 61  PPPHSPAALSPPDKFSPIHTIWKENRPQSVSVLMPGDEVPRFIALACPPCATAAAA--LV 120

Query: 121 DVLVQKPPQSTPDSS 128
           +++VQKP QS  DSS
Sbjct: 121 EIVVQKPSQSISDSS 133

[Close]

BLAST of Moc11g07140 vs. ExPASy TrEMBL
Match: A0A6J1F906 (uncharacterized protein At5g65660-like OS=Cucurbita moschata OX=3662 GN=LOC111443410 PE=4 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 8.3e-43
Identity = 96/137 (70.07%), Postives = 106/137 (77.37%), Query Frame = 0


Query: 1   MDYDDREAQDPASLSFPVGLVLLLTFFFCMCCFFSCCLHWDKLRSFLGCP---DHRHPPP 60
           M+YDDREAQD ASLSFPVGLVLLLTF FCMCCFFSCCLHW+KLRS LGCP   DHRH P 
Sbjct: 1   MEYDDREAQDSASLSFPVGLVLLLTFLFCMCCFFSCCLHWEKLRSILGCPDHADHRH-PS 60

Query: 61  IPPENAASSPPDKILPLHT----NQSQSLPVVMPGDEFPRFIAMACPPCAAAVAAAPFVD 120
           IPPE  A SPPDK  P+HT    N+ +SL V+MPGDE PRFIAMACPPC     AAP V+
Sbjct: 61  IPPETPAFSPPDKFSPIHTIWKENRPESLTVLMPGDEVPRFIAMACPPC----GAAPLVE 120

Query: 121 VLVQKPPQSTPDSSGAL 131
           +++QKP QS    SGAL
Sbjct: 121 IVIQKPSQSI---SGAL 129

[Close]

BLAST of Moc11g07140 vs. ExPASy TrEMBL
Match: A0A6J1IEI5 (uncharacterized protein At5g65660-like OS=Cucurbita maxima OX=3661 GN=LOC111476516 PE=4 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 4.1e-42
Identity = 94/136 (69.12%), Postives = 104/136 (76.47%), Query Frame = 0


Query: 1   MDYDDREAQDPASLSFPVGLVLLLTFFFCMCCFFSCCLHWDKLRSFLGCPDHRH--PPPI 60
           M+YDDREAQD ASLSFPVGLVLLLTF FCMCCFFSCCLHW+KLRS LGCPDH     P I
Sbjct: 1   MEYDDREAQDSASLSFPVGLVLLLTFLFCMCCFFSCCLHWEKLRSILGCPDHSDHLHPSI 60

Query: 61  PPENAASSPPDKILPLHT----NQSQSLPVVMPGDEFPRFIAMACPPCAAAVAAAPFVDV 120
           PPE  A SPPDK  P+HT    N+ +SL V+MPGDE PRFIAMACPPC     AAP V++
Sbjct: 61  PPETPAFSPPDKFSPIHTIWKENRPESLTVLMPGDEVPRFIAMACPPC----GAAPLVEI 120

Query: 121 LVQKPPQSTPDSSGAL 131
           ++QKP QS    SGAL
Sbjct: 121 VIQKPSQSI---SGAL 129

[Close]

BLAST of Moc11g07140 vs. TAIR 10
Match: AT5G65660.1 (hydroxyproline-rich glycoprotein family protein )

HSP 1 Score: 67.0 bits (162), Expect = 1.3e-11
Identity = 47/112 (41.96%), Postives = 57/112 (50.89%), Query Frame = 0


Query: 13  SLSFPVGLVLLLTFFFCMCCFFSCCLHWDKLRSFLGCPDHRHPPPIPPENAASSPPDKIL 72
           SL FP+G  LLL   F +   FSCC HWDK RS      +   P    E+    P     
Sbjct: 18  SLGFPLGTALLLIIIFSLSGIFSCCYHWDKHRSLRRSLANGR-PSADIESNPYKPKPPFP 77

Query: 73  PLHTNQSQSLPVVMPGDEFPRFIAMACPPCAAAVAAAPFVDVLVQKPPQSTP 125
            +   Q+ S+PV+MPGD  P+FIA+ C PCA        VD  VQ PPQS P
Sbjct: 78  EMKKPQNLSVPVLMPGDNTPKFIALPC-PCAPPRPEKLTVD--VQTPPQSPP 125

The following BLAST results are available for this feature:

BLAST of Moc11g07140 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022140744.1	4.3e-70	100.00	uncharacterized protein At5g65660-like [Momordica charantia]	[more]
XP_038899315.1	1.8e-47	74.63	uncharacterized protein At5g65660-like [Benincasa hispida]	[more]
XP_008466113.1	7.0e-44	71.11	PREDICTED: uncharacterized protein At5g65660-like [Cucumis melo] >KAA0038642.1 u...	[more]
XP_022936981.1	1.7e-42	70.07	uncharacterized protein At5g65660-like [Cucurbita moschata]	[more]
KAG6591711.1	5.0e-42	69.17	hypothetical protein SDJN03_14057, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of Moc11g07140 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LSK9	1.8e-10	41.96	Uncharacterized protein At5g65660 OS=Arabidopsis thaliana OX=3702 GN=At5g65660 P...	[more]

BLAST of Moc11g07140 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CGJ4	2.1e-70	100.00	uncharacterized protein At5g65660-like OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A5D3E545	3.4e-44	71.11	Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A1S3CQS4	3.4e-44	71.11	uncharacterized protein At5g65660-like OS=Cucumis melo OX=3656 GN=LOC103503633 P...	[more]
A0A6J1F906	8.3e-43	70.07	uncharacterized protein At5g65660-like OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A6J1IEI5	4.1e-42	69.12	uncharacterized protein At5g65660-like OS=Cucurbita maxima OX=3661 GN=LOC1114765...	[more]

BLAST of Moc11g07140 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G65660.1	1.3e-11	41.96	hydroxyproline-rich glycoprotein family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR34291:SF7	PROTEIN, PUTATIVE-RELATED	coord: 5..122
IPR037699	Uncharacterized protein At5g65660-like	PANTHER	PTHR34291	HYDROXYPROLINE-RICH GLYCOPROTEIN FAMILY PROTEIN	coord: 5..122

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc11g07140.1	Moc11g07140.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane