Moc11g06650 (gene) Bitter gourd (OHB3-1) v2

BLAST of Moc11g06650 vs. NCBI nr
Match: XP_022131344.1 (isoamylase 3, chloroplastic isoform X1 [Momordica charantia])

HSP 1 Score: 1619.0 bits (4191), Expect = 0.0e+00
Identity = 770/770 (100.00%), Postives = 770/770 (100.00%), Query Frame = 0

Query: 1   MMLRSPSLCHSSALHLPLFASCTGGSHFLHLRLKAGKRALGSSCREISGETKEGGAKHCV 60
           MMLRSPSLCHSSALHLPLFASCTGGSHFLHLRLKAGKRALGSSCREISGETKEGGAKHCV
Sbjct: 1   MMLRSPSLCHSSALHLPLFASCTGGSHFLHLRLKAGKRALGSSCREISGETKEGGAKHCV 60

Query: 61  VTAYARRDQDRVLEQEAPEILDTGPMLKMFPGEAFPLGVSEVDNGINFSIFSKHATSVTL 120
           VTAYARRDQDRVLEQEAPEILDTGPMLKMFPGEAFPLGVSEVDNGINFSIFSKHATSVTL
Sbjct: 61  VTAYARRDQDRVLEQEAPEILDTGPMLKMFPGEAFPLGVSEVDNGINFSIFSKHATSVTL 120

Query: 121 CLSLHERIDDRMLEFKLDPHVNRTGDVWHIRVQDLPRRNVLYGYRIDGPQGWHHGHRFDD 180
           CLSLHERIDDRMLEFKLDPHVNRTGDVWHIRVQDLPRRNVLYGYRIDGPQGWHHGHRFDD
Sbjct: 121 CLSLHERIDDRMLEFKLDPHVNRTGDVWHIRVQDLPRRNVLYGYRIDGPQGWHHGHRFDD 180

Query: 181 RIVLLDPYAKFVEGRRYFGGENKASGFLGTYDFGSMPFDWGDDYKLPNILEKDLVIYEMN 240
           RIVLLDPYAKFVEGRRYFGGENKASGFLGTYDFGSMPFDWGDDYKLPNILEKDLVIYEMN
Sbjct: 181 RIVLLDPYAKFVEGRRYFGGENKASGFLGTYDFGSMPFDWGDDYKLPNILEKDLVIYEMN 240

Query: 241 VRSFTADESSGLASSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRRSNPRDH 300
           VRSFTADESSGLASSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRRSNPRDH
Sbjct: 241 VRSFTADESSGLASSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRRSNPRDH 300

Query: 301 MINTWGYSTINFFAPMSRYASAGGGPLNASQEFKEMVKALHAAGIEVILDVVYNHTNEAD 360
           MINTWGYSTINFFAPMSRYASAGGGPLNASQEFKEMVKALHAAGIEVILDVVYNHTNEAD
Sbjct: 301 MINTWGYSTINFFAPMSRYASAGGGPLNASQEFKEMVKALHAAGIEVILDVVYNHTNEAD 360

Query: 361 DANPYTTSFRGIDNKVYYMLDLKNNGQLLNFSGCGNTLNCNHPVVMELVLDSLRHWVVEY 420
           DANPYTTSFRGIDNKVYYMLDLKNNGQLLNFSGCGNTLNCNHPVVMELVLDSLRHWVVEY
Sbjct: 361 DANPYTTSFRGIDNKVYYMLDLKNNGQLLNFSGCGNTLNCNHPVVMELVLDSLRHWVVEY 420

Query: 421 HVDGFRFDLASTLCRGTDGAPLSAPPLIREISKDAILSRCKIIAEPWDCGGLYLVGRFPN 480
           HVDGFRFDLASTLCRGTDGAPLSAPPLIREISKDAILSRCKIIAEPWDCGGLYLVGRFPN
Sbjct: 421 HVDGFRFDLASTLCRGTDGAPLSAPPLIREISKDAILSRCKIIAEPWDCGGLYLVGRFPN 480

Query: 481 WDRWAEWNGIYRDDVRKFIKGDCGMKGSFATRVAGSSDLYKVNKRKPYHGINFVIAHDGF 540
           WDRWAEWNGIYRDDVRKFIKGDCGMKGSFATRVAGSSDLYKVNKRKPYHGINFVIAHDGF
Sbjct: 481 WDRWAEWNGIYRDDVRKFIKGDCGMKGSFATRVAGSSDLYKVNKRKPYHGINFVIAHDGF 540

Query: 541 TLRDLVSYNVKHNDANGEGGNDGSNDNFSWNCGFEGETEDTSIKALRSRQMKNFHLALMT 600
           TLRDLVSYNVKHNDANGEGGNDGSNDNFSWNCGFEGETEDTSIKALRSRQMKNFHLALMT
Sbjct: 541 TLRDLVSYNVKHNDANGEGGNDGSNDNFSWNCGFEGETEDTSIKALRSRQMKNFHLALMT 600

Query: 601 SQGTPMMLMGDEYGHTRYGNNNSYGHDTAINHFQWGQLEARKRDHFRFFSEVIKFRREHP 660
           SQGTPMMLMGDEYGHTRYGNNNSYGHDTAINHFQWGQLEARKRDHFRFFSEVIKFRREHP
Sbjct: 601 SQGTPMMLMGDEYGHTRYGNNNSYGHDTAINHFQWGQLEARKRDHFRFFSEVIKFRREHP 660

Query: 661 QIFCRENFLHKDDITWHESNWDNPDSKFLAYTLHDDNGEDVYLAFNAHHYYVDVSLPSPP 720
           QIFCRENFLHKDDITWHESNWDNPDSKFLAYTLHDDNGEDVYLAFNAHHYYVDVSLPSPP
Sbjct: 661 QIFCRENFLHKDDITWHESNWDNPDSKFLAYTLHDDNGEDVYLAFNAHHYYVDVSLPSPP 720

Query: 721 TKRRWFRVVDTNLESPDDFVVDGIPSVGSTYNLAPYSSILLKAKLENEEL 771
           TKRRWFRVVDTNLESPDDFVVDGIPSVGSTYNLAPYSSILLKAKLENEEL
Sbjct: 721 TKRRWFRVVDTNLESPDDFVVDGIPSVGSTYNLAPYSSILLKAKLENEEL 770

BLAST of Moc11g06650 vs. NCBI nr
Match: XP_022131352.1 (isoamylase 3, chloroplastic isoform X2 [Momordica charantia])

HSP 1 Score: 1449.9 bits (3752), Expect = 0.0e+00
Identity = 685/685 (100.00%), Postives = 685/685 (100.00%), Query Frame = 0

Query: 86  MLKMFPGEAFPLGVSEVDNGINFSIFSKHATSVTLCLSLHERIDDRMLEFKLDPHVNRTG 145
           MLKMFPGEAFPLGVSEVDNGINFSIFSKHATSVTLCLSLHERIDDRMLEFKLDPHVNRTG
Sbjct: 1   MLKMFPGEAFPLGVSEVDNGINFSIFSKHATSVTLCLSLHERIDDRMLEFKLDPHVNRTG 60

Query: 146 DVWHIRVQDLPRRNVLYGYRIDGPQGWHHGHRFDDRIVLLDPYAKFVEGRRYFGGENKAS 205
           DVWHIRVQDLPRRNVLYGYRIDGPQGWHHGHRFDDRIVLLDPYAKFVEGRRYFGGENKAS
Sbjct: 61  DVWHIRVQDLPRRNVLYGYRIDGPQGWHHGHRFDDRIVLLDPYAKFVEGRRYFGGENKAS 120

Query: 206 GFLGTYDFGSMPFDWGDDYKLPNILEKDLVIYEMNVRSFTADESSGLASSTRGSYLGVIE 265
           GFLGTYDFGSMPFDWGDDYKLPNILEKDLVIYEMNVRSFTADESSGLASSTRGSYLGVIE
Sbjct: 121 GFLGTYDFGSMPFDWGDDYKLPNILEKDLVIYEMNVRSFTADESSGLASSTRGSYLGVIE 180

Query: 266 KIPHLLELGVNAVELLPVFEFDELEFQRRSNPRDHMINTWGYSTINFFAPMSRYASAGGG 325
           KIPHLLELGVNAVELLPVFEFDELEFQRRSNPRDHMINTWGYSTINFFAPMSRYASAGGG
Sbjct: 181 KIPHLLELGVNAVELLPVFEFDELEFQRRSNPRDHMINTWGYSTINFFAPMSRYASAGGG 240

Query: 326 PLNASQEFKEMVKALHAAGIEVILDVVYNHTNEADDANPYTTSFRGIDNKVYYMLDLKNN 385
           PLNASQEFKEMVKALHAAGIEVILDVVYNHTNEADDANPYTTSFRGIDNKVYYMLDLKNN
Sbjct: 241 PLNASQEFKEMVKALHAAGIEVILDVVYNHTNEADDANPYTTSFRGIDNKVYYMLDLKNN 300

Query: 386 GQLLNFSGCGNTLNCNHPVVMELVLDSLRHWVVEYHVDGFRFDLASTLCRGTDGAPLSAP 445
           GQLLNFSGCGNTLNCNHPVVMELVLDSLRHWVVEYHVDGFRFDLASTLCRGTDGAPLSAP
Sbjct: 301 GQLLNFSGCGNTLNCNHPVVMELVLDSLRHWVVEYHVDGFRFDLASTLCRGTDGAPLSAP 360

Query: 446 PLIREISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDDVRKFIKGDCGM 505
           PLIREISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDDVRKFIKGDCGM
Sbjct: 361 PLIREISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDDVRKFIKGDCGM 420

Query: 506 KGSFATRVAGSSDLYKVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGSN 565
           KGSFATRVAGSSDLYKVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGSN
Sbjct: 421 KGSFATRVAGSSDLYKVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGSN 480

Query: 566 DNFSWNCGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYG 625
           DNFSWNCGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYG
Sbjct: 481 DNFSWNCGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYG 540

Query: 626 HDTAINHFQWGQLEARKRDHFRFFSEVIKFRREHPQIFCRENFLHKDDITWHESNWDNPD 685
           HDTAINHFQWGQLEARKRDHFRFFSEVIKFRREHPQIFCRENFLHKDDITWHESNWDNPD
Sbjct: 541 HDTAINHFQWGQLEARKRDHFRFFSEVIKFRREHPQIFCRENFLHKDDITWHESNWDNPD 600

Query: 686 SKFLAYTLHDDNGEDVYLAFNAHHYYVDVSLPSPPTKRRWFRVVDTNLESPDDFVVDGIP 745
           SKFLAYTLHDDNGEDVYLAFNAHHYYVDVSLPSPPTKRRWFRVVDTNLESPDDFVVDGIP
Sbjct: 601 SKFLAYTLHDDNGEDVYLAFNAHHYYVDVSLPSPPTKRRWFRVVDTNLESPDDFVVDGIP 660

Query: 746 SVGSTYNLAPYSSILLKAKLENEEL 771
           SVGSTYNLAPYSSILLKAKLENEEL
Sbjct: 661 SVGSTYNLAPYSSILLKAKLENEEL 685

BLAST of Moc11g06650 vs. NCBI nr
Match: XP_022975928.1 (isoamylase 3, chloroplastic isoform X1 [Cucurbita maxima])

HSP 1 Score: 1424.5 bits (3686), Expect = 0.0e+00
Identity = 675/772 (87.44%), Postives = 717/772 (92.88%), Query Frame = 0

Query: 2   MLRSPSLCHSSALHLPLFA-SCTGGSHFLHLRLK-AGKRALG-SSCREISGETKEGGAKH 61
           M+RS  L HS+ L LPLFA SCTG +H +  RLK  GKR  G SS +E+  ETK+GG K 
Sbjct: 1   MIRSIPLHHSAPLQLPLFASSCTGAAHSVQFRLKPQGKRGPGSSSSQEVFSETKQGGVKI 60

Query: 62  CVVTAYARRDQDRVLEQEAPEILDTGPMLKMFPGEAFPLGVSEVDNGINFSIFSKHATSV 121
           C V+A+ RRDQD VLEQEAPE LDTGPMLK+ PG+AFPLGVSEVDNGINF+IFS+HAT+V
Sbjct: 61  CTVSAFGRRDQDHVLEQEAPENLDTGPMLKIVPGQAFPLGVSEVDNGINFAIFSQHATAV 120

Query: 122 TLCLSLHERIDDRMLEFKLDPHVNRTGDVWHIRVQDLPRRNVLYGYRIDGPQGWHHGHRF 181
           T+CLS +ERIDDR LEFKLDP  NRTGD+WHI +QDLPR+NVLYGYRIDGPQGWHHGHRF
Sbjct: 121 TICLSFNERIDDRTLEFKLDPDDNRTGDIWHICIQDLPRKNVLYGYRIDGPQGWHHGHRF 180

Query: 182 DDRIVLLDPYAKFVEGRRYFGGENKASGFLGTYDFGSMPFDWGDDYKLPNILEKDLVIYE 241
           DDRIVLLDPYAKFVEGRRYFGGENK+SGFLGTYDF S+PFDWGDDYKLPNILEKDLVIYE
Sbjct: 181 DDRIVLLDPYAKFVEGRRYFGGENKSSGFLGTYDFDSLPFDWGDDYKLPNILEKDLVIYE 240

Query: 242 MNVRSFTADESSGLASSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRRSNPR 301
           MNVR+FTADESSGLASS RGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQR  NPR
Sbjct: 241 MNVRAFTADESSGLASSLRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPR 300

Query: 302 DHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKEMVKALHAAGIEVILDVVYNHTNE 361
           +HMINTWGYSTINFFAPMSRYASAGGGPLNAS+EFKEMVKALH AGIEVILDVVYNHTNE
Sbjct: 301 NHMINTWGYSTINFFAPMSRYASAGGGPLNASREFKEMVKALHGAGIEVILDVVYNHTNE 360

Query: 362 ADDANPYTTSFRGIDNKVYYMLDLKNNGQLLNFSGCGNTLNCNHPVVMELVLDSLRHWVV 421
           ADD  PYTTSFRGIDNKVYYMLDL+NNGQ LNFSGCGNTLNCNHPVVMEL+LDSLRHWVV
Sbjct: 361 ADDVYPYTTSFRGIDNKVYYMLDLRNNGQFLNFSGCGNTLNCNHPVVMELILDSLRHWVV 420

Query: 422 EYHVDGFRFDLASTLCRGTDGAPLSAPPLIREISKDAILSRCKIIAEPWDCGGLYLVGRF 481
           EYHVDGFRFDLAS LCRG DGAPLSAPPLIR ISKDAILSRCKIIAEPWDCGGLYLVGRF
Sbjct: 421 EYHVDGFRFDLASVLCRGPDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLVGRF 480

Query: 482 PNWDRWAEWNGIYRDDVRKFIKGDCGMKGSFATRVAGSSDLYKVNKRKPYHGINFVIAHD 541
           PNWDRWAEWNGIYRDD+RKFIKGDCGMKGSFATRV+GSSDLYKVN RKPYHGINFVIAHD
Sbjct: 481 PNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVSGSSDLYKVNNRKPYHGINFVIAHD 540

Query: 542 GFTLRDLVSYNVKHNDANGEGGNDGSNDNFSWNCGFEGETEDTSIKALRSRQMKNFHLAL 601
           GFTLRDLVSYNVKHN+ANGEGGNDG NDNFSWNCGFEGETED +I+ALRSRQMKNFH+AL
Sbjct: 541 GFTLRDLVSYNVKHNNANGEGGNDGCNDNFSWNCGFEGETEDANIRALRSRQMKNFHMAL 600

Query: 602 MTSQGTPMMLMGDEYGHTRYGNNNSYGHDTAINHFQWGQLEARKRDHFRFFSEVIKFRRE 661
           MTSQGTPMMLMGDEYGHTRYGNNNSYGHDTA+NHF WGQLEARKRDHFRFFSEVIKFRR+
Sbjct: 601 MTSQGTPMMLMGDEYGHTRYGNNNSYGHDTALNHFLWGQLEARKRDHFRFFSEVIKFRRK 660

Query: 662 HPQIFCRENFLHKDDITWHESNWDNPDSKFLAYTLHDDNGEDVYLAFNAHHYYVDVSLPS 721
           HP +F RENFL ++DITWHESNWDNP+SKFLAYTLHD NGEDVYLAFNAH Y+V VSLPS
Sbjct: 661 HPHLFSRENFLGENDITWHESNWDNPESKFLAYTLHDGNGEDVYLAFNAHDYFVSVSLPS 720

Query: 722 PPTKRRWFRVVDTNLESPDDFVVDGIPSVGSTYNLAPYSSILLKAKLENEEL 771
           PPTKR+WFRVVDTNLESP DFV+DGI  +GSTYNLAPYSSILLKAKLENE L
Sbjct: 721 PPTKRKWFRVVDTNLESPQDFVLDGIRGIGSTYNLAPYSSILLKAKLENELL 772

BLAST of Moc11g06650 vs. NCBI nr
Match: XP_022936809.1 (isoamylase 3, chloroplastic isoform X1 [Cucurbita moschata])

HSP 1 Score: 1423.7 bits (3684), Expect = 0.0e+00
Identity = 676/774 (87.34%), Postives = 717/774 (92.64%), Query Frame = 0

Query: 2   MLRSPSLCHSSALHLPLFA-SCTGGSHFLHLRLK-AGKRALG---SSCREISGETKEGGA 61
           M+RS  L HS+ L LPLFA SCTG +H +  RLK  GKRA G   SS +E+  ETK+GG 
Sbjct: 1   MIRSIPLHHSAPLQLPLFASSCTGAAHSVQFRLKPQGKRAPGSSSSSSQEVFSETKQGGV 60

Query: 62  KHCVVTAYARRDQDRVLEQEAPEILDTGPMLKMFPGEAFPLGVSEVDNGINFSIFSKHAT 121
           K C V+A+ RRDQDRVLEQEAPE LDTGPMLK+ PG+AFPLGVSEVDNGINF+IFS+HAT
Sbjct: 61  KICTVSAFGRRDQDRVLEQEAPENLDTGPMLKVVPGQAFPLGVSEVDNGINFAIFSQHAT 120

Query: 122 SVTLCLSLHERIDDRMLEFKLDPHVNRTGDVWHIRVQDLPRRNVLYGYRIDGPQGWHHGH 181
           +VT+CLS +ERIDDR LEFKLDP  NRTGD+WHI +QDLPR+NVLYGYRIDGPQGWHHGH
Sbjct: 121 AVTICLSFNERIDDRTLEFKLDPDDNRTGDIWHICIQDLPRKNVLYGYRIDGPQGWHHGH 180

Query: 182 RFDDRIVLLDPYAKFVEGRRYFGGENKASGFLGTYDFGSMPFDWGDDYKLPNILEKDLVI 241
           RFDD IVLLDPYAKFVEGRRYFGGENK+SGFLGTYDF S+PFDWGDDYKLPNILEKDLVI
Sbjct: 181 RFDDSIVLLDPYAKFVEGRRYFGGENKSSGFLGTYDFDSLPFDWGDDYKLPNILEKDLVI 240

Query: 242 YEMNVRSFTADESSGLASSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRRSN 301
           YEMNVR+FTADESSGLASS RGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQR  N
Sbjct: 241 YEMNVRAFTADESSGLASSIRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPN 300

Query: 302 PRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKEMVKALHAAGIEVILDVVYNHT 361
           PR+HMINTWGYSTINFFAPMSRYASAGGGPLNAS+EFKEMVKALH AGIEVILDVVYNHT
Sbjct: 301 PRNHMINTWGYSTINFFAPMSRYASAGGGPLNASREFKEMVKALHGAGIEVILDVVYNHT 360

Query: 362 NEADDANPYTTSFRGIDNKVYYMLDLKNNGQLLNFSGCGNTLNCNHPVVMELVLDSLRHW 421
           NEADD  PYTTSFRGIDNKVYYMLDL+NNGQ LNFSGCGNTLNCNHPVVMEL+LDSLRHW
Sbjct: 361 NEADDVYPYTTSFRGIDNKVYYMLDLRNNGQFLNFSGCGNTLNCNHPVVMELILDSLRHW 420

Query: 422 VVEYHVDGFRFDLASTLCRGTDGAPLSAPPLIREISKDAILSRCKIIAEPWDCGGLYLVG 481
           VVEYHVDGFRFDLAS LCRG DGAPLSAPPLIR ISKDAILSRCKIIAEPWDCGGLYLVG
Sbjct: 421 VVEYHVDGFRFDLASVLCRGPDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLVG 480

Query: 482 RFPNWDRWAEWNGIYRDDVRKFIKGDCGMKGSFATRVAGSSDLYKVNKRKPYHGINFVIA 541
           RFPNWDRWAEWNGIYRDD+RKFIKGDCGMKGSFATRV+GSSDLYKVN RKPYHGINFVIA
Sbjct: 481 RFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVSGSSDLYKVNNRKPYHGINFVIA 540

Query: 542 HDGFTLRDLVSYNVKHNDANGEGGNDGSNDNFSWNCGFEGETEDTSIKALRSRQMKNFHL 601
           HDGFTLRDLVSYNVKHN+ANGEGGNDG NDNFSWNCGFEGETED +I+ALRSRQMKNFHL
Sbjct: 541 HDGFTLRDLVSYNVKHNNANGEGGNDGCNDNFSWNCGFEGETEDANIRALRSRQMKNFHL 600

Query: 602 ALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDTAINHFQWGQLEARKRDHFRFFSEVIKFR 661
           ALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDTA+NHF WGQLEARKRDHFRFFSEVIKFR
Sbjct: 601 ALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDTALNHFLWGQLEARKRDHFRFFSEVIKFR 660

Query: 662 REHPQIFCRENFLHKDDITWHESNWDNPDSKFLAYTLHDDNGEDVYLAFNAHHYYVDVSL 721
           R+HP +F RENFL ++DITWHESNWDNP+SKFLAYTLHD NGEDVYLAFNAH Y+V VSL
Sbjct: 661 RKHPHLFSRENFLGENDITWHESNWDNPESKFLAYTLHDGNGEDVYLAFNAHDYFVSVSL 720

Query: 722 PSPPTKRRWFRVVDTNLESPDDFVVDGIPSVGSTYNLAPYSSILLKAKLENEEL 771
           PSPPT R+WFRVVDTNLESP DFV+DGI  +GSTYNLAPYSSILLKAKLENE L
Sbjct: 721 PSPPTNRKWFRVVDTNLESPQDFVLDGIRGIGSTYNLAPYSSILLKAKLENELL 774

BLAST of Moc11g06650 vs. NCBI nr
Match: XP_023535183.1 (isoamylase 3, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1420.6 bits (3676), Expect = 0.0e+00
Identity = 674/772 (87.31%), Postives = 716/772 (92.75%), Query Frame = 0

Query: 2   MLRSPSLCHSSALHLPLFA-SCTGGSHFLHLRLK-AGKRALG-SSCREISGETKEGGAKH 61
           M+RS  L HS+ L LPLFA SCTG +H +  RLK  GKRA G SS +E+  E K+GG K 
Sbjct: 1   MIRSIPLHHSAPLQLPLFASSCTGAAHSVQFRLKPQGKRAPGSSSSQEVFSEAKQGGVKI 60

Query: 62  CVVTAYARRDQDRVLEQEAPEILDTGPMLKMFPGEAFPLGVSEVDNGINFSIFSKHATSV 121
           C V+A+ RRDQDRVLEQEAPE LDTGPMLK+ PG+AFPLGVSEVDNGINF+IFS+HAT+V
Sbjct: 61  CTVSAFGRRDQDRVLEQEAPENLDTGPMLKIVPGQAFPLGVSEVDNGINFAIFSQHATAV 120

Query: 122 TLCLSLHERIDDRMLEFKLDPHVNRTGDVWHIRVQDLPRRNVLYGYRIDGPQGWHHGHRF 181
           T+CLS +ERIDDR LEFKLDP  NRTGD+WHI +QDLPR+NVLYGYRIDGPQGWHHGHRF
Sbjct: 121 TICLSFNERIDDRTLEFKLDPDDNRTGDIWHICIQDLPRKNVLYGYRIDGPQGWHHGHRF 180

Query: 182 DDRIVLLDPYAKFVEGRRYFGGENKASGFLGTYDFGSMPFDWGDDYKLPNILEKDLVIYE 241
           DD IVLLDPYAKFVEGRRYFGGENK+SGFLGTYDF S+PFDWGDDYKLPNILEKDLVIYE
Sbjct: 181 DDSIVLLDPYAKFVEGRRYFGGENKSSGFLGTYDFDSLPFDWGDDYKLPNILEKDLVIYE 240

Query: 242 MNVRSFTADESSGLASSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRRSNPR 301
           MNVR+FTADESSGLASS RGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQR  NPR
Sbjct: 241 MNVRAFTADESSGLASSIRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPR 300

Query: 302 DHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKEMVKALHAAGIEVILDVVYNHTNE 361
           +HMINTWGYSTINFFAPMSRYASAGGGPLNAS+EFKEMVKALH AGIEVILDVVYNHTNE
Sbjct: 301 NHMINTWGYSTINFFAPMSRYASAGGGPLNASREFKEMVKALHGAGIEVILDVVYNHTNE 360

Query: 362 ADDANPYTTSFRGIDNKVYYMLDLKNNGQLLNFSGCGNTLNCNHPVVMELVLDSLRHWVV 421
           ADD  PYTTSFRGIDNKVYYMLDL+NNGQ LNFSGCGNTLNCNHPVVMEL+LDSLRHWVV
Sbjct: 361 ADDVYPYTTSFRGIDNKVYYMLDLRNNGQFLNFSGCGNTLNCNHPVVMELILDSLRHWVV 420

Query: 422 EYHVDGFRFDLASTLCRGTDGAPLSAPPLIREISKDAILSRCKIIAEPWDCGGLYLVGRF 481
           EYHVDGFRFDLAS LCRG DGAPLSAPPLIR ISKD ILSRCKIIAEPWDCGGLYLVGRF
Sbjct: 421 EYHVDGFRFDLASVLCRGPDGAPLSAPPLIRAISKDDILSRCKIIAEPWDCGGLYLVGRF 480

Query: 482 PNWDRWAEWNGIYRDDVRKFIKGDCGMKGSFATRVAGSSDLYKVNKRKPYHGINFVIAHD 541
           PNWDRWAEWNGIYRDD+RKFIKGDCGMKGSFATRV+GSSDLYKVN RKP+HGINFVIAHD
Sbjct: 481 PNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVSGSSDLYKVNNRKPFHGINFVIAHD 540

Query: 542 GFTLRDLVSYNVKHNDANGEGGNDGSNDNFSWNCGFEGETEDTSIKALRSRQMKNFHLAL 601
           GFTLRDLVSYNVKHN+ANGEGGNDG NDNFSWNCGFEGETED +I+ALRSRQMKNFHLAL
Sbjct: 541 GFTLRDLVSYNVKHNNANGEGGNDGCNDNFSWNCGFEGETEDANIRALRSRQMKNFHLAL 600

Query: 602 MTSQGTPMMLMGDEYGHTRYGNNNSYGHDTAINHFQWGQLEARKRDHFRFFSEVIKFRRE 661
           MTSQGTPMMLMGDEYGHTRYGNNNSYGHDTA+NHF WGQLEARKRDHFRFFSEVIKFRR+
Sbjct: 601 MTSQGTPMMLMGDEYGHTRYGNNNSYGHDTALNHFLWGQLEARKRDHFRFFSEVIKFRRK 660

Query: 662 HPQIFCRENFLHKDDITWHESNWDNPDSKFLAYTLHDDNGEDVYLAFNAHHYYVDVSLPS 721
           HP +F RENFL ++DITWHESNWDNP+SKFLAYTLHD NGEDVYLAFNAH Y+V VSLPS
Sbjct: 661 HPHLFSRENFLGENDITWHESNWDNPESKFLAYTLHDGNGEDVYLAFNAHDYFVSVSLPS 720

Query: 722 PPTKRRWFRVVDTNLESPDDFVVDGIPSVGSTYNLAPYSSILLKAKLENEEL 771
           PPTKR+WFRVVDTNLESP DFV+DGI  +GSTYNLAPYSSILLKAKLENE L
Sbjct: 721 PPTKRKWFRVVDTNLESPQDFVLDGIRGIGSTYNLAPYSSILLKAKLENELL 772

BLAST of Moc11g06650 vs. ExPASy Swiss-Prot
Match: Q9M0S5 (Isoamylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA3 PE=1 SV=2)

HSP 1 Score: 1119.4 bits (2894), Expect = 0.0e+00
Identity = 542/774 (70.03%), Postives = 622/774 (80.36%), Query Frame = 0

Query: 2   MLRSPSLCHS----SALHLPLFASCTGGSHFLHLRLKAGKRALGSSCREISGETKEGGAK 61
           ML SPS   +    S+   P   +    S  + + LK  +R   +  R  S + K+    
Sbjct: 1   MLTSPSSSSTYDPFSSNFSPSLTNAFSSSFTIPMGLKLSRRV--TRARIFSRKIKD---- 60

Query: 62  HCVVTAYARRDQDRVLEQEAPEILDTGPMLKMFPGEAFPLGVSEVDNGINFSIFSKHATS 121
              +    RR  +RV+E+EA  + +T  + K+  GE  PLGVS+VD GINF++FS++ATS
Sbjct: 61  RSTLKVTCRRAHERVVEEEASTMTET-KLFKVSSGEVSPLGVSQVDKGINFALFSQNATS 120

Query: 122 VTLCLSL-----HERIDDRMLEFKLDPHVNRTGDVWHIRVQDLPRRNVLYGYRIDGPQGW 181
           VTLCLSL      +  DD M+E  LDP VN+TGD WHI V+DLP  NVLYGYR+DGP  W
Sbjct: 121 VTLCLSLSQSGKDDTDDDGMIELVLDPSVNKTGDTWHICVEDLPLNNVLYGYRVDGPGEW 180

Query: 182 HHGHRFDDRIVLLDPYAKFVEGRRYFG-GENKASGFLGTYDFGSMPFDWGDDYKLPNILE 241
             GHRFD  I+LLDPYAK V+G   FG    K + F GTYDF S PFDWGDDYK PNI E
Sbjct: 181 QQGHRFDRSILLLDPYAKLVKGHSSFGDSSQKFAQFYGTYDFESSPFDWGDDYKFPNIPE 240

Query: 242 KDLVIYEMNVRSFTADESSGLASSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEF 301
           KDLVIYEMNVR+FTADESSG+  +  GSYLG IEKIPHL +LG+NAVELLPVFEFDELE 
Sbjct: 241 KDLVIYEMNVRAFTADESSGMDPAIGGSYLGFIEKIPHLQDLGINAVELLPVFEFDELEL 300

Query: 302 QRRSNPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKEMVKALHAAGIEVILDV 361
           QRRSNPRDHM+NTWGYST+NFFAPMSRYAS  G P+ AS+EFKEMVKALH+AGIEVILDV
Sbjct: 301 QRRSNPRDHMVNTWGYSTVNFFAPMSRYASGEGDPIKASKEFKEMVKALHSAGIEVILDV 360

Query: 362 VYNHTNEADDANPYTTSFRGIDNKVYYMLDLKNNGQLLNFSGCGNTLNCNHPVVMELVLD 421
           VYNHTNEADD  PYTTSFRGIDNKVYYMLD   N QLLNFSGCGNTLNCNHPVVMEL+LD
Sbjct: 361 VYNHTNEADDKYPYTTSFRGIDNKVYYMLD--PNNQLLNFSGCGNTLNCNHPVVMELILD 420

Query: 422 SLRHWVVEYHVDGFRFDLASTLCRGTDGAPLSAPPLIREISKDAILSRCKIIAEPWDCGG 481
           SLRHWV EYHVDGFRFDLAS LCR TDG+PLSAPPLIR I+KD++LSRCKIIAEPWDCGG
Sbjct: 421 SLRHWVTEYHVDGFRFDLASVLCRATDGSPLSAPPLIRAIAKDSVLSRCKIIAEPWDCGG 480

Query: 482 LYLVGRFPNWDRWAEWNGIYRDDVRKFIKGDCGMKGSFATRVAGSSDLYKVNKRKPYHGI 541
           LYLVG+FPNWDRWAEWNG+YRDDVR+FIKGD GMKGSFATRV+GSSDLY+VN+RKPYHG+
Sbjct: 481 LYLVGKFPNWDRWAEWNGMYRDDVRRFIKGDSGMKGSFATRVSGSSDLYQVNQRKPYHGV 540

Query: 542 NFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGSNDNFSWNCGFEGETEDTSIKALRSRQM 601
           NFVIAHDGFTLRDLVSYN KHN+ANGEGGNDG NDN SWNCGFEGET D  IK+LR+RQM
Sbjct: 541 NFVIAHDGFTLRDLVSYNFKHNEANGEGGNDGCNDNHSWNCGFEGETGDAHIKSLRTRQM 600

Query: 602 KNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDTAINHFQWGQLEARKRDHFRFFSE 661
           KNFHLALM SQGTPMMLMGDEYGHTRYGNNNSYGHDT++N+FQW +L+A+K++HFRFFSE
Sbjct: 601 KNFHLALMISQGTPMMLMGDEYGHTRYGNNNSYGHDTSLNNFQWKELDAKKQNHFRFFSE 660

Query: 662 VIKFRREHPQIFCRENFLHKDDITWHESNWDNPDSKFLAYTLHDD-NGEDVYLAFNAHHY 721
           VIKFR  H  +   ENFL + +ITWHE NWDN +SKFLA+TLHD   G D+Y+AFNAH Y
Sbjct: 661 VIKFRHSH-HVLKHENFLTQGEITWHEDNWDNSESKFLAFTLHDGIGGRDIYVAFNAHDY 720

Query: 722 YVDVSLPSPPTKRRWFRVVDTNLESPDDFVVDGIPSVGSTYNLAPYSSILLKAK 765
           +V   +P PP  ++WFRV DTNLESPDDFV +G+  V  TYN+AP+SSILL++K
Sbjct: 721 FVKALIPQPPPGKQWFRVADTNLESPDDFVREGVAGVADTYNVAPFSSILLQSK 764

BLAST of Moc11g06650 vs. ExPASy Swiss-Prot
Match: B9G434 (Isoamylase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA3 PE=2 SV=1)

HSP 1 Score: 1096.6 bits (2835), Expect = 0.0e+00
Identity = 515/698 (73.78%), Postives = 588/698 (84.24%), Query Frame = 0

Query: 74  EQEAPEILDTGPMLKMFPGEAFPLGVSEVDNGINFSIFSKHATSVTLCLSLHER--IDDR 133
           E+    + DT    K   G+AFPLGVS+V+ G+NF++FS+HA+SV LCL L  R   D++
Sbjct: 85  EERGCTMSDTEMPFKYSSGKAFPLGVSQVEGGLNFALFSQHASSVILCLKLPGRGTEDEK 144

Query: 134 ---MLEFKLDPHVNRTGDVWHIRVQDLPRRNVLYGYRIDGPQGWHHGHRFDDRIVLLDPY 193
              ++EF LD   N+TGD+WH+ V+ LP   VLYGYR+ GPQGW  GHRFD   VLLDPY
Sbjct: 145 GADVVEFVLDQQKNKTGDIWHVIVEGLPASGVLYGYRVGGPQGWDQGHRFDSSTVLLDPY 204

Query: 194 AKFVEGRRYFG-GENKASGFLGTYDFGSMPFDWGDDYKLPNILEKDLVIYEMNVRSFTAD 253
           AK V GR+YFG  E K+S   GTYDF S PFDWGDDY+LPN+ E DLVIYEMNVR+FTAD
Sbjct: 205 AKLVSGRKYFGVAEEKSSQHFGTYDFDSSPFDWGDDYRLPNLPEADLVIYEMNVRAFTAD 264

Query: 254 ESSGLASSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRRSNPRDHMINTWGY 313
           ESSGL S++RGSYLG+I+KIPHLLELGVNAVELLPVFE+DELEF+R  NPRDHM+NTWGY
Sbjct: 265 ESSGLDSTSRGSYLGLIDKIPHLLELGVNAVELLPVFEYDELEFKRYPNPRDHMVNTWGY 324

Query: 314 STINFFAPMSRYASAGGGPLNASQEFKEMVKALHAAGIEVILDVVYNHTNEADDANPYTT 373
           STINFFAPMSRYASAGGGP+ AS+E K+MVK LH AGIEVILDVVYNHTNEADDA+PY T
Sbjct: 325 STINFFAPMSRYASAGGGPVAASKELKQMVKELHKAGIEVILDVVYNHTNEADDAHPYMT 384

Query: 374 SFRGIDNKVYYMLDLKNNGQLLNFSGCGNTLNCNHPVVMELVLDSLRHWVVEYHVDGFRF 433
           SFRGIDNKVYYMLDL  N +LLNFSGCGNTLNCNHPVV EL+LDSLRHWV EYH+DGFRF
Sbjct: 385 SFRGIDNKVYYMLDLNKNAELLNFSGCGNTLNCNHPVVKELILDSLRHWVEEYHIDGFRF 444

Query: 434 DLASTLCRGTDGAPLSAPPLIREISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEW 493
           DLAS LCRG DG PL APPLI+EI+KDA+LSRCKIIAEPWDCGGLYLVGRFPNWDRWAEW
Sbjct: 445 DLASVLCRGPDGCPLDAPPLIKEIAKDAVLSRCKIIAEPWDCGGLYLVGRFPNWDRWAEW 504

Query: 494 NGIYRDDVRKFIKGDCGMKGSFATRVAGSSDLYKVNKRKPYHGINFVIAHDGFTLRDLVS 553
           NG YRDD+R+FIKGD GMKG FATRV+GS+DLY+VN+RKPYHG+NFVIAHDGFTL DLVS
Sbjct: 505 NGKYRDDLRRFIKGDPGMKGVFATRVSGSADLYQVNERKPYHGVNFVIAHDGFTLCDLVS 564

Query: 554 YNVKHNDANGEGGNDGSNDNFSWNCGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMM 613
           YN+KHNDANGEGG DG NDNFSWNCG EGET D ++ +LRSRQMKNFH+ALM SQGTPMM
Sbjct: 565 YNLKHNDANGEGGCDGCNDNFSWNCGVEGETNDLNVLSLRSRQMKNFHVALMISQGTPMM 624

Query: 614 LMGDEYGHTRYGNNNSYGHDTAINHFQWGQLEARKRDHFRFFSEVIKFRREHPQIFCREN 673
           LMGDEYGHTRYGNNNSYGHDT IN+FQW QLE R+  HFRFFSE+IKFR  +P I  R+ 
Sbjct: 625 LMGDEYGHTRYGNNNSYGHDTCINNFQWEQLEQRRDGHFRFFSEMIKFRHSNP-ILRRDR 684

Query: 674 FLHKDDITWHESNWDNPDSKFLAYTLHDDN-GEDVYLAFNAHHYYVDVSLPSPPTKRRWF 733
           FL+K+D+TWHE  W+N +SKFLA+T+HD N G D+YLAFNAH Y+VD  +P PP  + W 
Sbjct: 685 FLNKNDVTWHEDCWENQESKFLAFTVHDHNSGGDIYLAFNAHDYFVDAVIPPPPHHKCWN 744

Query: 734 RVVDTNLESPDDFVVDGIPSVGSTYNLAPYSSILLKAK 765
           RVVDTNLESP+D V +G+P  G  Y +APYSSILLKAK
Sbjct: 745 RVVDTNLESPNDIVPEGVPFTGPKYRIAPYSSILLKAK 781

BLAST of Moc11g06650 vs. ExPASy Swiss-Prot
Match: D0TZF0 (Isoamylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA1 PE=1 SV=1)

HSP 1 Score: 606.7 bits (1563), Expect = 3.7e-172
Identity = 343/769 (44.60%), Postives = 446/769 (58.00%), Query Frame = 0

Query: 37  KRALGSSCREISGETKEGGAKHCVVTAYARRDQDRVLEQEAPEILDTGPMLKMFPGEAFP 96
           +R++ S+     GE +E G +          +++ V     PE    G   ++  G   P
Sbjct: 53  RRSVASAVEVGVGEDEEEGVE---------EEEEEVEAVVMPERYALGGACRVLAGMPAP 112

Query: 97  LGVSEVDNGINFSIFSKHATSVTLCLSLHERI--DDRMLEFKLDPHVNRTGDVWHIRVQD 156
           LG + +D G+NF+++S  A++ +LCL   + +  D+   E  LDP  NRTG+VWH+ ++ 
Sbjct: 113 LGATALDGGVNFAVYSAGASAASLCLFTPDDLEADEVTEEVPLDPLFNRTGNVWHVFIEG 172

Query: 157 LPRRNVLYGYRIDGPQGWHHGHRFDDRIVLLDPYAKFVEGRRYFG----GENKASGFLGT 216
               N+LYGYR DG    H G  FD   V++DPYAK V  R  +G    G +      G 
Sbjct: 173 -ELHNMLYGYRFDGMFAPHCGQYFDVSNVVVDPYAKAVISRGEYGVPGPGGDCWPQMAGM 232

Query: 217 YDFGSMPFDWGDDYKLPNILEKDLVIYEMNVRSFTADESSGLASSTRGSYLGVIEKIPHL 276
                  FDW  D  L    +KDLVIYEM++R FT   SS +     G+Y+G I K+ +L
Sbjct: 233 IPLPYSTFDWQGDLPL-RYPQKDLVIYEMHLRGFTKHSSSNVEHP--GTYIGAISKLDYL 292

Query: 277 LELGVNAVELLPVFEFDELEFQRRSNPRDHMINTWGYSTINFFAPMSRYASAG--GGPLN 336
            ELGVN VEL+P  EF+ELE+   S+     +N WGYSTINFF+PM RY+S G      +
Sbjct: 293 KELGVNCVELMPCHEFNELEYFSCSS----KMNFWGYSTINFFSPMIRYSSGGIRNCGRD 352

Query: 337 ASQEFKEMVKALHAAGIEVILDVVYNHTNEADDANPYTTSFRGIDNKVYYMLDLKNNGQL 396
           A  EFK  V+  H  GIEVI+DVV+NHT E ++  P   SFRGIDN  YYML  K  G+ 
Sbjct: 353 AINEFKTFVREAHKRGIEVIMDVVFNHTAEGNEKGP-ILSFRGIDNSTYYMLAPK--GEF 412

Query: 397 LNFSGCGNTLNCNHPVVMELVLDSLRHWVVEYHVDGFRFDLASTLCRG------------ 456
            N+SGCGNT NCNHPVV E ++D LR+WV E HVDGFRFDLAS + RG            
Sbjct: 413 YNYSGCGNTFNCNHPVVREFIVDCLRYWVTEMHVDGFRFDLASIMTRGCSLWDPVNVYGS 472

Query: 457 -------TDGAPLSAPPLIREISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNG 516
                  T G PL+ PPLI  IS D IL   K+IAE WD GGLY VG+FP+W  W+EWNG
Sbjct: 473 PVEGDMTTTGTPLATPPLIDMISNDPILGDVKLIAEAWDAGGLYQVGQFPHWKIWSEWNG 532

Query: 517 IYRDDVRKFIKGDCGMKGSFATRVAGSSDLYKVNKRKPYHGINFVIAHDGFTLRDLVSYN 576
            YRD VR+FIKG  G  G FA  + GS  LY+   RKP+H INFV AHDGFTL DLV+YN
Sbjct: 533 KYRDIVRQFIKGTDGFAGGFAECLCGSPHLYQAGGRKPWHSINFVCAHDGFTLADLVTYN 592

Query: 577 VKHNDANGEGGNDGSNDNFSWNCGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLM 636
            K+N +NGE   DG N N SWNCG EGE    S+K LR RQM+NF ++LM SQG PM  M
Sbjct: 593 KKYNSSNGEDNRDGENHNLSWNCGEEGEFAGLSVKRLRKRQMRNFFVSLMVSQGVPMFYM 652

Query: 637 GDEYGHTRYGNNNSYGHDTAINHFQWGQLEARKRDHFRFFSEVIKFRREHPQIFCRENFL 696
           GDEYGHT+ GNNN+Y HD  +N+F+W + E    D  RF S + KFR++   +   + F 
Sbjct: 653 GDEYGHTKGGNNNTYCHDHYVNYFRWDKKE-ESSDLQRFCSLMTKFRKQCESLGLAD-FP 712

Query: 697 HKDDITWHESNWDNPD----SKFLAYTLHDDNGEDVYLAFNAHHYYVDVSLPSPPTKRRW 756
               + WH      PD    S+F+A++  D+   ++Y+AFNA H    V LP  P   RW
Sbjct: 713 TAQRLHWHGHQPGKPDWSETSRFVAFSTKDETKGEIYVAFNASHLPAVVGLPERP-GYRW 772

Query: 757 FRVVDTNLESPDDFVVDGIPS------------VGSTYNLAPYSSILLK 763
             +VDT   +P DF+ D +P               + Y +  YSSI+L+
Sbjct: 773 EPLVDTGKPAPYDFLTDDLPDRAHAVHLFSHFLNSNLYPMLSYSSIILE 798

BLAST of Moc11g06650 vs. ExPASy Swiss-Prot
Match: O04196 (Isoamylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA1 PE=1 SV=1)

HSP 1 Score: 600.9 bits (1548), Expect = 2.0e-170
Identity = 333/711 (46.84%), Postives = 429/711 (60.34%), Query Frame = 0

Query: 96  PLGVSEVDNGINFSIFSKHATSVTLCL-SLHERIDDRML-EFKLDPHVNRTGDVWHIRVQ 155
           P G +  D+G+NFS++S ++ S T+CL SL +   +++  E +LDP  NRTG VWH+ ++
Sbjct: 76  PFGPTVRDDGVNFSVYSTNSVSATICLISLSDLRQNKVTEEIQLDPSRNRTGHVWHVFLR 135

Query: 156 DLPRRNVLYGYRIDGPQGWHHGHRFDDRIVLLDPYAKFVEGRRYFG----GENKASGFLG 215
               +++LYGYR DG      GH +D   +LLDPYAK +  R  FG     +N       
Sbjct: 136 G-DFKDMLYGYRFDGKFSPEEGHYYDSSNILLDPYAKAIISRDEFGVLGPDDNCWPQMAC 195

Query: 216 TYDFGSMPFDWGDDYKLPNILEKDLVIYEMNVRSFTADESSGLASSTRGSYLGVIEKIPH 275
                   FDW  D  L  + +KDLVIYEM+VR FT  ESS +     G+Y GV EK+ H
Sbjct: 196 MVPTREEEFDWEGDMHL-KLPQKDLVIYEMHVRGFTRHESSKI--EFPGTYQGVAEKLDH 255

Query: 276 LLELGVNAVELLPVFEFDELEFQRRSN-PRDHMINTWGYSTINFFAPMSRYASAGGGPL- 335
           L ELG+N +EL+P  EF+ELE+   +    DH +N WGYSTI FF+PM RYASA      
Sbjct: 256 LKELGINCIELMPCHEFNELEYYSYNTILGDHRVNFWGYSTIGFFSPMIRYASASSNNFA 315

Query: 336 -NASQEFKEMVKALHAAGIEVILDVVYNHTNEADDANPYTTSFRGIDNKVYYMLDLKNNG 395
             A  EFK +VK  H  GIEVI+DVV NHT E ++  P   SFRG+DN VYYML  K  G
Sbjct: 316 GRAINEFKILVKEAHKRGIEVIMDVVLNHTAEGNEKGP-IFSFRGVDNSVYYMLAPK--G 375

Query: 396 QLLNFSGCGNTLNCNHPVVMELVLDSLRHWVVEYHVDGFRFDLASTLCRG---------- 455
           +  N+SGCGNT NCNHPVV + +LD LR+WV E HVDGFRFDL S + R           
Sbjct: 376 EFYNYSGCGNTFNCNHPVVRQFILDCLRYWVTEMHVDGFRFDLGSIMSRSSSLWDAANVY 435

Query: 456 ---------TDGAPLSAPPLIREISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEW 515
                    T G P+S PP+I  IS D IL   K+IAE WD GGLY VG FP+W  W+EW
Sbjct: 436 GADVEGDLLTTGTPISCPPVIDMISNDPILRGVKLIAEAWDAGGLYQVGMFPHWGIWSEW 495

Query: 516 NGIYRDDVRKFIKGDCGMKGSFATRVAGSSDLYKVNKRKPYHGINFVIAHDGFTLRDLVS 575
           NG +RD VR+FIKG  G  G+FA  + GS +LY+   RKP+H INF+ AHDGFTL DLV+
Sbjct: 496 NGKFRDVVRQFIKGTDGFSGAFAECLCGSPNLYQ-GGRKPWHSINFICAHDGFTLADLVT 555

Query: 576 YNVKHNDANGEGGNDGSNDNFSWNCGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMM 635
           YN K+N ANGE  NDG N N+SWNCG EG+    S+K LR RQM+NF ++LM SQG PM+
Sbjct: 556 YNNKNNLANGEENNDGENHNYSWNCGEEGDFASISVKRLRKRQMRNFFVSLMVSQGVPMI 615

Query: 636 LMGDEYGHTRYGNNNSYGHDTAINHFQWGQLEARKRDHFRFFSEVIKFRREHPQIFCREN 695
            MGDEYGHT+ GNNN+Y HD  +N+F+W + E    D FRF   +IKFR E   +   + 
Sbjct: 616 YMGDEYGHTKGGNNNTYCHDNYMNYFRWDKKEEAHSDFFRFCRILIKFRDECESLGLND- 675

Query: 696 FLHKDDITWHE-----SNWDNPDSKFLAYTLHDDNGEDVYLAFNAHHYYVDVSLPSPPTK 755
           F     + WH       NW +  S+F+A++L D   +++Y+AFN  H    VSLP+ P  
Sbjct: 676 FPTAKRLQWHGLAPEIPNW-SETSRFVAFSLVDSVKKEIYVAFNTSHLATLVSLPNRP-G 735

Query: 756 RRWFRVVDTNLESPDDFVVDGIPS------------VGSTYNLAPYSSILL 762
            RW   VDT+  SP D +   +P               + Y +  YSSI+L
Sbjct: 736 YRWEPFVDTSKPSPYDCITPDLPERETAMKQYRHFLDANVYPMLSYSSIIL 775

BLAST of Moc11g06650 vs. ExPASy Swiss-Prot
Match: P0A4Y5 (Glycogen operon protein GlgX homolog OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=glgX PE=3 SV=1)

HSP 1 Score: 461.8 bits (1187), Expect = 1.5e-128
Identity = 302/735 (41.09%), Postives = 397/735 (54.01%), Query Frame = 0

Query: 83  TGPML-KMFPGEAFPLGVSEVDNGINFSIFSKHATSVTLCLSLHERIDDRMLEFKLDPHV 142
           TGP L  ++PG A+PLG +    G NFS+FS+ A  V LCL   + ++ R+   ++D   
Sbjct: 12  TGPALPTVWPGNAYPLGATYDGAGTNFSLFSEIAEKVELCLIDEDGVESRIPLDEVD--- 71

Query: 143 NRTGDVWHIRVQDLPRRNVLYGYRIDGPQGWHHGHRFDDRIVLLDPYAKFVEGRRYFGGE 202
              G VWH  + ++      YG+R+ GP     GHR D   +LLDPY K   G   F G+
Sbjct: 72  ---GYVWHAYLPNI-TPGQRYGFRVHGPFDPAAGHRCDPSKLLLDPYGKSFHGDFTF-GQ 131

Query: 203 NKASGFLGTYDFGSMP------------------FDWGDDYKLPNILEKDLVIYEMNVRS 262
              S  +   D  S P                  FDW  D + P     + VIYE +V+ 
Sbjct: 132 ALYSYDVNAVDPDSTPPMVDSLGHTMTSVVINPFFDWAYD-RSPRTPYHETVIYEAHVKG 191

Query: 263 FTADESSGLASSTRGSYLGVIEK--IPHLLELGVNAVELLPVFEFDELEFQRRSNPRDHM 322
            T    S +    RG+Y G+     I HL EL V AVEL+PV +F  L   R  +    +
Sbjct: 192 MTQTHPS-IPPELRGTYAGLAHPVIIDHLNELNVTAVELMPVHQF--LHDSRLLDL--GL 251

Query: 323 INTWGYSTINFFAPMSRYASAGGGPLNASQEFKEMVKALHAAGIEVILDVVYNHTNEADD 382
            N WGY+T  FFAP  +YAS      +A  EFK MV++LH AGIEVILDVVYNHT E + 
Sbjct: 252 RNYWGYNTFGFFAPHHQYASTRQAG-SAVAEFKTMVRSLHEAGIEVILDVVYNHTAEGNH 311

Query: 383 ANPYTTSFRGIDNKVYYMLDLKNNGQLLNFSGCGNTLNCNHPVVMELVLDSLRHWVVEYH 442
             P T +FRGIDN  YY L   +     +F+G GN+LN  HP  ++L++DSLR+WV+E H
Sbjct: 312 LGP-TINFRGIDNTAYYRLMDHDLRFYKDFTGTGNSLNARHPHTLQLIMDSLRYWVIEMH 371

Query: 443 VDGFRFDLASTLCRGT-DGAPLSAPPLIREISKDAILSRCKIIAEPWDCG-GLYLVGRFP 502
           VDGFRFDLASTL R   D   LSA      + +D ++S+ K+IAEPWD G G Y VG FP
Sbjct: 372 VDGFRFDLASTLARELHDVDRLSA--FFDLVQQDPVVSQVKLIAEPWDVGEGGYQVGNFP 431

Query: 503 NWDRWAEWNGIYRDDVRKFIKGDCGMKGSFATRVAGSSDLYKVNKRKPYHGINFVIAHDG 562
               W EWNG YRD VR + +G+    G FA+R+ GSSDLY+   R+P   INFV AHDG
Sbjct: 432 G--LWTEWNGKYRDTVRDYWRGEPATLGEFASRLTGSSDLYEATGRRPSASINFVTAHDG 491

Query: 563 FTLRDLVSYNVKHNDANGEGGNDGSNDNFSWNCGFEGETEDTSIKALRSRQMKNFHLALM 622
           FTL DLVSYN KHN+ANGE   DG + N SWNCG EG T+D  I ALR+RQM+N    LM
Sbjct: 492 FTLNDLVSYNDKHNEANGENNRDGESYNRSWNCGVEGPTDDPDILALRARQMRNMWATLM 551

Query: 623 TSQGTPMMLMGDEYGHTRYGNNNSYGHDTAINHFQWGQLEARKRDHFRFFSEVIKFRREH 682
            SQGTPM+  GDE G T+YGNNN Y  D+ ++   W  ++ +  D   F  +    R+ H
Sbjct: 552 VSQGTPMIAHGDEIGRTQYGNNNVYCQDSELSWMDWSLVD-KNADLLAFARKATTLRKNH 611

Query: 683 PQIFCRENFLHKD---------DITW---------HES---NWDNPDSKFL---AYTLHD 742
            ++F R  F   +         DI W         HE     +D   + FL   A T  D
Sbjct: 612 -KVFRRRRFFEGEPIRSGDEVRDIAWLTPSGREMTHEDWGRGFDRCVAVFLNGEAITAPD 671

Query: 743 DNGEDV-----YLAFNAHHYYVDVSLPSPPTKRRWFRVVDTNLESPDDFVVDGIPSVGST 766
             GE V      L FNAH + V+  +P     ++W   +DTN    D   +D   +   T
Sbjct: 672 ARGERVVDDSFLLCFNAHDHDVEFVMPHDGYAQQWTGELDTNDPVGD---IDLTVTATDT 721

BLAST of Moc11g06650 vs. ExPASy TrEMBL
Match: A0A6J1BQ05 (isoamylase 3, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111004593 PE=4 SV=1)

HSP 1 Score: 1619.0 bits (4191), Expect = 0.0e+00
Identity = 770/770 (100.00%), Postives = 770/770 (100.00%), Query Frame = 0

Query: 1   MMLRSPSLCHSSALHLPLFASCTGGSHFLHLRLKAGKRALGSSCREISGETKEGGAKHCV 60
           MMLRSPSLCHSSALHLPLFASCTGGSHFLHLRLKAGKRALGSSCREISGETKEGGAKHCV
Sbjct: 1   MMLRSPSLCHSSALHLPLFASCTGGSHFLHLRLKAGKRALGSSCREISGETKEGGAKHCV 60

Query: 61  VTAYARRDQDRVLEQEAPEILDTGPMLKMFPGEAFPLGVSEVDNGINFSIFSKHATSVTL 120
           VTAYARRDQDRVLEQEAPEILDTGPMLKMFPGEAFPLGVSEVDNGINFSIFSKHATSVTL
Sbjct: 61  VTAYARRDQDRVLEQEAPEILDTGPMLKMFPGEAFPLGVSEVDNGINFSIFSKHATSVTL 120

Query: 121 CLSLHERIDDRMLEFKLDPHVNRTGDVWHIRVQDLPRRNVLYGYRIDGPQGWHHGHRFDD 180
           CLSLHERIDDRMLEFKLDPHVNRTGDVWHIRVQDLPRRNVLYGYRIDGPQGWHHGHRFDD
Sbjct: 121 CLSLHERIDDRMLEFKLDPHVNRTGDVWHIRVQDLPRRNVLYGYRIDGPQGWHHGHRFDD 180

Query: 181 RIVLLDPYAKFVEGRRYFGGENKASGFLGTYDFGSMPFDWGDDYKLPNILEKDLVIYEMN 240
           RIVLLDPYAKFVEGRRYFGGENKASGFLGTYDFGSMPFDWGDDYKLPNILEKDLVIYEMN
Sbjct: 181 RIVLLDPYAKFVEGRRYFGGENKASGFLGTYDFGSMPFDWGDDYKLPNILEKDLVIYEMN 240

Query: 241 VRSFTADESSGLASSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRRSNPRDH 300
           VRSFTADESSGLASSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRRSNPRDH
Sbjct: 241 VRSFTADESSGLASSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRRSNPRDH 300

Query: 301 MINTWGYSTINFFAPMSRYASAGGGPLNASQEFKEMVKALHAAGIEVILDVVYNHTNEAD 360
           MINTWGYSTINFFAPMSRYASAGGGPLNASQEFKEMVKALHAAGIEVILDVVYNHTNEAD
Sbjct: 301 MINTWGYSTINFFAPMSRYASAGGGPLNASQEFKEMVKALHAAGIEVILDVVYNHTNEAD 360

Query: 361 DANPYTTSFRGIDNKVYYMLDLKNNGQLLNFSGCGNTLNCNHPVVMELVLDSLRHWVVEY 420
           DANPYTTSFRGIDNKVYYMLDLKNNGQLLNFSGCGNTLNCNHPVVMELVLDSLRHWVVEY
Sbjct: 361 DANPYTTSFRGIDNKVYYMLDLKNNGQLLNFSGCGNTLNCNHPVVMELVLDSLRHWVVEY 420

Query: 421 HVDGFRFDLASTLCRGTDGAPLSAPPLIREISKDAILSRCKIIAEPWDCGGLYLVGRFPN 480
           HVDGFRFDLASTLCRGTDGAPLSAPPLIREISKDAILSRCKIIAEPWDCGGLYLVGRFPN
Sbjct: 421 HVDGFRFDLASTLCRGTDGAPLSAPPLIREISKDAILSRCKIIAEPWDCGGLYLVGRFPN 480

Query: 481 WDRWAEWNGIYRDDVRKFIKGDCGMKGSFATRVAGSSDLYKVNKRKPYHGINFVIAHDGF 540
           WDRWAEWNGIYRDDVRKFIKGDCGMKGSFATRVAGSSDLYKVNKRKPYHGINFVIAHDGF
Sbjct: 481 WDRWAEWNGIYRDDVRKFIKGDCGMKGSFATRVAGSSDLYKVNKRKPYHGINFVIAHDGF 540

Query: 541 TLRDLVSYNVKHNDANGEGGNDGSNDNFSWNCGFEGETEDTSIKALRSRQMKNFHLALMT 600
           TLRDLVSYNVKHNDANGEGGNDGSNDNFSWNCGFEGETEDTSIKALRSRQMKNFHLALMT
Sbjct: 541 TLRDLVSYNVKHNDANGEGGNDGSNDNFSWNCGFEGETEDTSIKALRSRQMKNFHLALMT 600

Query: 601 SQGTPMMLMGDEYGHTRYGNNNSYGHDTAINHFQWGQLEARKRDHFRFFSEVIKFRREHP 660
           SQGTPMMLMGDEYGHTRYGNNNSYGHDTAINHFQWGQLEARKRDHFRFFSEVIKFRREHP
Sbjct: 601 SQGTPMMLMGDEYGHTRYGNNNSYGHDTAINHFQWGQLEARKRDHFRFFSEVIKFRREHP 660

Query: 661 QIFCRENFLHKDDITWHESNWDNPDSKFLAYTLHDDNGEDVYLAFNAHHYYVDVSLPSPP 720
           QIFCRENFLHKDDITWHESNWDNPDSKFLAYTLHDDNGEDVYLAFNAHHYYVDVSLPSPP
Sbjct: 661 QIFCRENFLHKDDITWHESNWDNPDSKFLAYTLHDDNGEDVYLAFNAHHYYVDVSLPSPP 720

Query: 721 TKRRWFRVVDTNLESPDDFVVDGIPSVGSTYNLAPYSSILLKAKLENEEL 771
           TKRRWFRVVDTNLESPDDFVVDGIPSVGSTYNLAPYSSILLKAKLENEEL
Sbjct: 721 TKRRWFRVVDTNLESPDDFVVDGIPSVGSTYNLAPYSSILLKAKLENEEL 770

BLAST of Moc11g06650 vs. ExPASy TrEMBL
Match: A0A6J1BQS3 (isoamylase 3, chloroplastic isoform X2 OS=Momordica charantia OX=3673 GN=LOC111004593 PE=4 SV=1)

HSP 1 Score: 1449.9 bits (3752), Expect = 0.0e+00
Identity = 685/685 (100.00%), Postives = 685/685 (100.00%), Query Frame = 0

Query: 86  MLKMFPGEAFPLGVSEVDNGINFSIFSKHATSVTLCLSLHERIDDRMLEFKLDPHVNRTG 145
           MLKMFPGEAFPLGVSEVDNGINFSIFSKHATSVTLCLSLHERIDDRMLEFKLDPHVNRTG
Sbjct: 1   MLKMFPGEAFPLGVSEVDNGINFSIFSKHATSVTLCLSLHERIDDRMLEFKLDPHVNRTG 60

Query: 146 DVWHIRVQDLPRRNVLYGYRIDGPQGWHHGHRFDDRIVLLDPYAKFVEGRRYFGGENKAS 205
           DVWHIRVQDLPRRNVLYGYRIDGPQGWHHGHRFDDRIVLLDPYAKFVEGRRYFGGENKAS
Sbjct: 61  DVWHIRVQDLPRRNVLYGYRIDGPQGWHHGHRFDDRIVLLDPYAKFVEGRRYFGGENKAS 120

Query: 206 GFLGTYDFGSMPFDWGDDYKLPNILEKDLVIYEMNVRSFTADESSGLASSTRGSYLGVIE 265
           GFLGTYDFGSMPFDWGDDYKLPNILEKDLVIYEMNVRSFTADESSGLASSTRGSYLGVIE
Sbjct: 121 GFLGTYDFGSMPFDWGDDYKLPNILEKDLVIYEMNVRSFTADESSGLASSTRGSYLGVIE 180

Query: 266 KIPHLLELGVNAVELLPVFEFDELEFQRRSNPRDHMINTWGYSTINFFAPMSRYASAGGG 325
           KIPHLLELGVNAVELLPVFEFDELEFQRRSNPRDHMINTWGYSTINFFAPMSRYASAGGG
Sbjct: 181 KIPHLLELGVNAVELLPVFEFDELEFQRRSNPRDHMINTWGYSTINFFAPMSRYASAGGG 240

Query: 326 PLNASQEFKEMVKALHAAGIEVILDVVYNHTNEADDANPYTTSFRGIDNKVYYMLDLKNN 385
           PLNASQEFKEMVKALHAAGIEVILDVVYNHTNEADDANPYTTSFRGIDNKVYYMLDLKNN
Sbjct: 241 PLNASQEFKEMVKALHAAGIEVILDVVYNHTNEADDANPYTTSFRGIDNKVYYMLDLKNN 300

Query: 386 GQLLNFSGCGNTLNCNHPVVMELVLDSLRHWVVEYHVDGFRFDLASTLCRGTDGAPLSAP 445
           GQLLNFSGCGNTLNCNHPVVMELVLDSLRHWVVEYHVDGFRFDLASTLCRGTDGAPLSAP
Sbjct: 301 GQLLNFSGCGNTLNCNHPVVMELVLDSLRHWVVEYHVDGFRFDLASTLCRGTDGAPLSAP 360

Query: 446 PLIREISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDDVRKFIKGDCGM 505
           PLIREISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDDVRKFIKGDCGM
Sbjct: 361 PLIREISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDDVRKFIKGDCGM 420

Query: 506 KGSFATRVAGSSDLYKVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGSN 565
           KGSFATRVAGSSDLYKVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGSN
Sbjct: 421 KGSFATRVAGSSDLYKVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGSN 480

Query: 566 DNFSWNCGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYG 625
           DNFSWNCGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYG
Sbjct: 481 DNFSWNCGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYG 540

Query: 626 HDTAINHFQWGQLEARKRDHFRFFSEVIKFRREHPQIFCRENFLHKDDITWHESNWDNPD 685
           HDTAINHFQWGQLEARKRDHFRFFSEVIKFRREHPQIFCRENFLHKDDITWHESNWDNPD
Sbjct: 541 HDTAINHFQWGQLEARKRDHFRFFSEVIKFRREHPQIFCRENFLHKDDITWHESNWDNPD 600

Query: 686 SKFLAYTLHDDNGEDVYLAFNAHHYYVDVSLPSPPTKRRWFRVVDTNLESPDDFVVDGIP 745
           SKFLAYTLHDDNGEDVYLAFNAHHYYVDVSLPSPPTKRRWFRVVDTNLESPDDFVVDGIP
Sbjct: 601 SKFLAYTLHDDNGEDVYLAFNAHHYYVDVSLPSPPTKRRWFRVVDTNLESPDDFVVDGIP 660

Query: 746 SVGSTYNLAPYSSILLKAKLENEEL 771
           SVGSTYNLAPYSSILLKAKLENEEL
Sbjct: 661 SVGSTYNLAPYSSILLKAKLENEEL 685

BLAST of Moc11g06650 vs. ExPASy TrEMBL
Match: A0A6J1IKM9 (isoamylase 3, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111476487 PE=4 SV=1)

HSP 1 Score: 1424.5 bits (3686), Expect = 0.0e+00
Identity = 675/772 (87.44%), Postives = 717/772 (92.88%), Query Frame = 0

Query: 2   MLRSPSLCHSSALHLPLFA-SCTGGSHFLHLRLK-AGKRALG-SSCREISGETKEGGAKH 61
           M+RS  L HS+ L LPLFA SCTG +H +  RLK  GKR  G SS +E+  ETK+GG K 
Sbjct: 1   MIRSIPLHHSAPLQLPLFASSCTGAAHSVQFRLKPQGKRGPGSSSSQEVFSETKQGGVKI 60

Query: 62  CVVTAYARRDQDRVLEQEAPEILDTGPMLKMFPGEAFPLGVSEVDNGINFSIFSKHATSV 121
           C V+A+ RRDQD VLEQEAPE LDTGPMLK+ PG+AFPLGVSEVDNGINF+IFS+HAT+V
Sbjct: 61  CTVSAFGRRDQDHVLEQEAPENLDTGPMLKIVPGQAFPLGVSEVDNGINFAIFSQHATAV 120

Query: 122 TLCLSLHERIDDRMLEFKLDPHVNRTGDVWHIRVQDLPRRNVLYGYRIDGPQGWHHGHRF 181
           T+CLS +ERIDDR LEFKLDP  NRTGD+WHI +QDLPR+NVLYGYRIDGPQGWHHGHRF
Sbjct: 121 TICLSFNERIDDRTLEFKLDPDDNRTGDIWHICIQDLPRKNVLYGYRIDGPQGWHHGHRF 180

Query: 182 DDRIVLLDPYAKFVEGRRYFGGENKASGFLGTYDFGSMPFDWGDDYKLPNILEKDLVIYE 241
           DDRIVLLDPYAKFVEGRRYFGGENK+SGFLGTYDF S+PFDWGDDYKLPNILEKDLVIYE
Sbjct: 181 DDRIVLLDPYAKFVEGRRYFGGENKSSGFLGTYDFDSLPFDWGDDYKLPNILEKDLVIYE 240

Query: 242 MNVRSFTADESSGLASSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRRSNPR 301
           MNVR+FTADESSGLASS RGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQR  NPR
Sbjct: 241 MNVRAFTADESSGLASSLRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPR 300

Query: 302 DHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKEMVKALHAAGIEVILDVVYNHTNE 361
           +HMINTWGYSTINFFAPMSRYASAGGGPLNAS+EFKEMVKALH AGIEVILDVVYNHTNE
Sbjct: 301 NHMINTWGYSTINFFAPMSRYASAGGGPLNASREFKEMVKALHGAGIEVILDVVYNHTNE 360

Query: 362 ADDANPYTTSFRGIDNKVYYMLDLKNNGQLLNFSGCGNTLNCNHPVVMELVLDSLRHWVV 421
           ADD  PYTTSFRGIDNKVYYMLDL+NNGQ LNFSGCGNTLNCNHPVVMEL+LDSLRHWVV
Sbjct: 361 ADDVYPYTTSFRGIDNKVYYMLDLRNNGQFLNFSGCGNTLNCNHPVVMELILDSLRHWVV 420

Query: 422 EYHVDGFRFDLASTLCRGTDGAPLSAPPLIREISKDAILSRCKIIAEPWDCGGLYLVGRF 481
           EYHVDGFRFDLAS LCRG DGAPLSAPPLIR ISKDAILSRCKIIAEPWDCGGLYLVGRF
Sbjct: 421 EYHVDGFRFDLASVLCRGPDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLVGRF 480

Query: 482 PNWDRWAEWNGIYRDDVRKFIKGDCGMKGSFATRVAGSSDLYKVNKRKPYHGINFVIAHD 541
           PNWDRWAEWNGIYRDD+RKFIKGDCGMKGSFATRV+GSSDLYKVN RKPYHGINFVIAHD
Sbjct: 481 PNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVSGSSDLYKVNNRKPYHGINFVIAHD 540

Query: 542 GFTLRDLVSYNVKHNDANGEGGNDGSNDNFSWNCGFEGETEDTSIKALRSRQMKNFHLAL 601
           GFTLRDLVSYNVKHN+ANGEGGNDG NDNFSWNCGFEGETED +I+ALRSRQMKNFH+AL
Sbjct: 541 GFTLRDLVSYNVKHNNANGEGGNDGCNDNFSWNCGFEGETEDANIRALRSRQMKNFHMAL 600

Query: 602 MTSQGTPMMLMGDEYGHTRYGNNNSYGHDTAINHFQWGQLEARKRDHFRFFSEVIKFRRE 661
           MTSQGTPMMLMGDEYGHTRYGNNNSYGHDTA+NHF WGQLEARKRDHFRFFSEVIKFRR+
Sbjct: 601 MTSQGTPMMLMGDEYGHTRYGNNNSYGHDTALNHFLWGQLEARKRDHFRFFSEVIKFRRK 660

Query: 662 HPQIFCRENFLHKDDITWHESNWDNPDSKFLAYTLHDDNGEDVYLAFNAHHYYVDVSLPS 721
           HP +F RENFL ++DITWHESNWDNP+SKFLAYTLHD NGEDVYLAFNAH Y+V VSLPS
Sbjct: 661 HPHLFSRENFLGENDITWHESNWDNPESKFLAYTLHDGNGEDVYLAFNAHDYFVSVSLPS 720

Query: 722 PPTKRRWFRVVDTNLESPDDFVVDGIPSVGSTYNLAPYSSILLKAKLENEEL 771
           PPTKR+WFRVVDTNLESP DFV+DGI  +GSTYNLAPYSSILLKAKLENE L
Sbjct: 721 PPTKRKWFRVVDTNLESPQDFVLDGIRGIGSTYNLAPYSSILLKAKLENELL 772

BLAST of Moc11g06650 vs. ExPASy TrEMBL
Match: A0A6J1F9D1 (isoamylase 3, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443282 PE=4 SV=1)

HSP 1 Score: 1423.7 bits (3684), Expect = 0.0e+00
Identity = 676/774 (87.34%), Postives = 717/774 (92.64%), Query Frame = 0

Query: 2   MLRSPSLCHSSALHLPLFA-SCTGGSHFLHLRLK-AGKRALG---SSCREISGETKEGGA 61
           M+RS  L HS+ L LPLFA SCTG +H +  RLK  GKRA G   SS +E+  ETK+GG 
Sbjct: 1   MIRSIPLHHSAPLQLPLFASSCTGAAHSVQFRLKPQGKRAPGSSSSSSQEVFSETKQGGV 60

Query: 62  KHCVVTAYARRDQDRVLEQEAPEILDTGPMLKMFPGEAFPLGVSEVDNGINFSIFSKHAT 121
           K C V+A+ RRDQDRVLEQEAPE LDTGPMLK+ PG+AFPLGVSEVDNGINF+IFS+HAT
Sbjct: 61  KICTVSAFGRRDQDRVLEQEAPENLDTGPMLKVVPGQAFPLGVSEVDNGINFAIFSQHAT 120

Query: 122 SVTLCLSLHERIDDRMLEFKLDPHVNRTGDVWHIRVQDLPRRNVLYGYRIDGPQGWHHGH 181
           +VT+CLS +ERIDDR LEFKLDP  NRTGD+WHI +QDLPR+NVLYGYRIDGPQGWHHGH
Sbjct: 121 AVTICLSFNERIDDRTLEFKLDPDDNRTGDIWHICIQDLPRKNVLYGYRIDGPQGWHHGH 180

Query: 182 RFDDRIVLLDPYAKFVEGRRYFGGENKASGFLGTYDFGSMPFDWGDDYKLPNILEKDLVI 241
           RFDD IVLLDPYAKFVEGRRYFGGENK+SGFLGTYDF S+PFDWGDDYKLPNILEKDLVI
Sbjct: 181 RFDDSIVLLDPYAKFVEGRRYFGGENKSSGFLGTYDFDSLPFDWGDDYKLPNILEKDLVI 240

Query: 242 YEMNVRSFTADESSGLASSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRRSN 301
           YEMNVR+FTADESSGLASS RGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQR  N
Sbjct: 241 YEMNVRAFTADESSGLASSIRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPN 300

Query: 302 PRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKEMVKALHAAGIEVILDVVYNHT 361
           PR+HMINTWGYSTINFFAPMSRYASAGGGPLNAS+EFKEMVKALH AGIEVILDVVYNHT
Sbjct: 301 PRNHMINTWGYSTINFFAPMSRYASAGGGPLNASREFKEMVKALHGAGIEVILDVVYNHT 360

Query: 362 NEADDANPYTTSFRGIDNKVYYMLDLKNNGQLLNFSGCGNTLNCNHPVVMELVLDSLRHW 421
           NEADD  PYTTSFRGIDNKVYYMLDL+NNGQ LNFSGCGNTLNCNHPVVMEL+LDSLRHW
Sbjct: 361 NEADDVYPYTTSFRGIDNKVYYMLDLRNNGQFLNFSGCGNTLNCNHPVVMELILDSLRHW 420

Query: 422 VVEYHVDGFRFDLASTLCRGTDGAPLSAPPLIREISKDAILSRCKIIAEPWDCGGLYLVG 481
           VVEYHVDGFRFDLAS LCRG DGAPLSAPPLIR ISKDAILSRCKIIAEPWDCGGLYLVG
Sbjct: 421 VVEYHVDGFRFDLASVLCRGPDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLVG 480

Query: 482 RFPNWDRWAEWNGIYRDDVRKFIKGDCGMKGSFATRVAGSSDLYKVNKRKPYHGINFVIA 541
           RFPNWDRWAEWNGIYRDD+RKFIKGDCGMKGSFATRV+GSSDLYKVN RKPYHGINFVIA
Sbjct: 481 RFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVSGSSDLYKVNNRKPYHGINFVIA 540

Query: 542 HDGFTLRDLVSYNVKHNDANGEGGNDGSNDNFSWNCGFEGETEDTSIKALRSRQMKNFHL 601
           HDGFTLRDLVSYNVKHN+ANGEGGNDG NDNFSWNCGFEGETED +I+ALRSRQMKNFHL
Sbjct: 541 HDGFTLRDLVSYNVKHNNANGEGGNDGCNDNFSWNCGFEGETEDANIRALRSRQMKNFHL 600

Query: 602 ALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDTAINHFQWGQLEARKRDHFRFFSEVIKFR 661
           ALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDTA+NHF WGQLEARKRDHFRFFSEVIKFR
Sbjct: 601 ALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDTALNHFLWGQLEARKRDHFRFFSEVIKFR 660

Query: 662 REHPQIFCRENFLHKDDITWHESNWDNPDSKFLAYTLHDDNGEDVYLAFNAHHYYVDVSL 721
           R+HP +F RENFL ++DITWHESNWDNP+SKFLAYTLHD NGEDVYLAFNAH Y+V VSL
Sbjct: 661 RKHPHLFSRENFLGENDITWHESNWDNPESKFLAYTLHDGNGEDVYLAFNAHDYFVSVSL 720

Query: 722 PSPPTKRRWFRVVDTNLESPDDFVVDGIPSVGSTYNLAPYSSILLKAKLENEEL 771
           PSPPT R+WFRVVDTNLESP DFV+DGI  +GSTYNLAPYSSILLKAKLENE L
Sbjct: 721 PSPPTNRKWFRVVDTNLESPQDFVLDGIRGIGSTYNLAPYSSILLKAKLENELL 774

BLAST of Moc11g06650 vs. ExPASy TrEMBL
Match: A0A1S3CS22 (isoamylase 3, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503684 PE=4 SV=1)

HSP 1 Score: 1415.2 bits (3662), Expect = 0.0e+00
Identity = 671/769 (87.26%), Postives = 709/769 (92.20%), Query Frame = 0

Query: 2   MLRSPSLCHSSALHLPLF-ASCTGGSHFLHLRLKAGKRAL----GSSCREISGETKEGGA 61
           M+RS  L HS+ L  PLF +SCTG +HFLH RLK   + +     S  REI  ETK+GG 
Sbjct: 1   MIRSLPLHHSTTLQFPLFPSSCTGATHFLHFRLKRQVKLVSGSSSSDSREIFSETKQGGV 60

Query: 62  KHCVVTAYARRDQDRVLEQEAPEILDTGPMLKMFPGEAFPLGVSEVDNGINFSIFSKHAT 121
           K   V+A++RRDQDRVLEQEAPE LDTGPMLK+FPG+AFPLGVSEVDNGINF+IFS+HAT
Sbjct: 61  KLWTVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHAT 120

Query: 122 SVTLCLSLHERIDDRMLEFKLDPHVNRTGDVWHIRVQDLPRRNVLYGYRIDGPQGWHHGH 181
           SVT+CLSL  RIDD MLEFKLDP  NRTGD+WHI +QDL R+NVLYGYRIDGPQGWHHGH
Sbjct: 121 SVTICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQDLQRKNVLYGYRIDGPQGWHHGH 180

Query: 182 RFDDRIVLLDPYAKFVEGRRYFGGENKASGFLGTYDFGSMPFDWGDDYKLPNILEKDLVI 241
           R+DD  +LLDPYAKFVEGRR+FGGENK+SGFLGTYDF S+PFDWG+DYKLPNI EKDLVI
Sbjct: 181 RYDDGTMLLDPYAKFVEGRRFFGGENKSSGFLGTYDFESLPFDWGNDYKLPNIPEKDLVI 240

Query: 242 YEMNVRSFTADESSGLASSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRRSN 301
           YEMNVR FTADESSGL SSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQR  N
Sbjct: 241 YEMNVRGFTADESSGLPSSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPN 300

Query: 302 PRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKEMVKALHAAGIEVILDVVYNHT 361
           PRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFK+MVKALHAAGIEVILDVVYNHT
Sbjct: 301 PRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKQMVKALHAAGIEVILDVVYNHT 360

Query: 362 NEADDANPYTTSFRGIDNKVYYMLDLKNNGQLLNFSGCGNTLNCNHPVVMELVLDSLRHW 421
           NE DD NPYTTSFRGIDNKVYYMLDLKNNGQ  NFSGCGNTLNCNHPVVMEL+L+SLRHW
Sbjct: 361 NEGDDVNPYTTSFRGIDNKVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESLRHW 420

Query: 422 VVEYHVDGFRFDLASTLCRGTDGAPLSAPPLIREISKDAILSRCKIIAEPWDCGGLYLVG 481
           VVEYHVDGFRFDLAS LCRGTDGAPLSAPPLIR ISKDAILSRCKIIAEPWDCGGLYLVG
Sbjct: 421 VVEYHVDGFRFDLASVLCRGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLVG 480

Query: 482 RFPNWDRWAEWNGIYRDDVRKFIKGDCGMKGSFATRVAGSSDLYKVNKRKPYHGINFVIA 541
           RFPNWDRWAEWNGIYRDD+RKFIKGDCGMKGSFATRVAGSSDLY VNKRKPYHGINFVIA
Sbjct: 481 RFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVAGSSDLYNVNKRKPYHGINFVIA 540

Query: 542 HDGFTLRDLVSYNVKHNDANGEGGNDGSNDNFSWNCGFEGETEDTSIKALRSRQMKNFHL 601
           HDGFTLRDLVSYNVKHNDANGEGGNDG NDNFSWNCGFEGETEDTSIKALRSRQMKNFHL
Sbjct: 541 HDGFTLRDLVSYNVKHNDANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRSRQMKNFHL 600

Query: 602 ALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDTAINHFQWGQLEARKRDHFRFFSEVIKFR 661
           ALMTSQGTPMMLMGDEYGHTRYGNNNSYGHD A+NHF W QLEARKRDHFRFFSEVIKFR
Sbjct: 601 ALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFFSEVIKFR 660

Query: 662 REHPQIFCRENFLHKDDITWHESNWDNPDSKFLAYTLHDDNGEDVYLAFNAHHYYVDVSL 721
           R+HP +F RENFL+K+DITWHESNWDNP+SKFLAYTLHDDNGEDVYLAFNAH Y+V+VSL
Sbjct: 661 RKHPHLFSRENFLNKNDITWHESNWDNPESKFLAYTLHDDNGEDVYLAFNAHDYFVNVSL 720

Query: 722 PSPPTKRRWFRVVDTNLESPDDFVVDGIPSVGSTYNLAPYSSILLKAKL 766
           PSPPT RRWFRVVDTNLESP DFV+DGIP VGS+YNLAPYSSILLKA L
Sbjct: 721 PSPPTNRRWFRVVDTNLESPHDFVLDGIPGVGSSYNLAPYSSILLKANL 769

BLAST of Moc11g06650 vs. TAIR 10
Match: AT4G09020.1 (isoamylase 3 )

HSP 1 Score: 1119.4 bits (2894), Expect = 0.0e+00
Identity = 542/774 (70.03%), Postives = 622/774 (80.36%), Query Frame = 0

Query: 2   MLRSPSLCHS----SALHLPLFASCTGGSHFLHLRLKAGKRALGSSCREISGETKEGGAK 61
           ML SPS   +    S+   P   +    S  + + LK  +R   +  R  S + K+    
Sbjct: 1   MLTSPSSSSTYDPFSSNFSPSLTNAFSSSFTIPMGLKLSRRV--TRARIFSRKIKD---- 60

Query: 62  HCVVTAYARRDQDRVLEQEAPEILDTGPMLKMFPGEAFPLGVSEVDNGINFSIFSKHATS 121
              +    RR  +RV+E+EA  + +T  + K+  GE  PLGVS+VD GINF++FS++ATS
Sbjct: 61  RSTLKVTCRRAHERVVEEEASTMTET-KLFKVSSGEVSPLGVSQVDKGINFALFSQNATS 120

Query: 122 VTLCLSL-----HERIDDRMLEFKLDPHVNRTGDVWHIRVQDLPRRNVLYGYRIDGPQGW 181
           VTLCLSL      +  DD M+E  LDP VN+TGD WHI V+DLP  NVLYGYR+DGP  W
Sbjct: 121 VTLCLSLSQSGKDDTDDDGMIELVLDPSVNKTGDTWHICVEDLPLNNVLYGYRVDGPGEW 180

Query: 182 HHGHRFDDRIVLLDPYAKFVEGRRYFG-GENKASGFLGTYDFGSMPFDWGDDYKLPNILE 241
             GHRFD  I+LLDPYAK V+G   FG    K + F GTYDF S PFDWGDDYK PNI E
Sbjct: 181 QQGHRFDRSILLLDPYAKLVKGHSSFGDSSQKFAQFYGTYDFESSPFDWGDDYKFPNIPE 240

Query: 242 KDLVIYEMNVRSFTADESSGLASSTRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEF 301
           KDLVIYEMNVR+FTADESSG+  +  GSYLG IEKIPHL +LG+NAVELLPVFEFDELE 
Sbjct: 241 KDLVIYEMNVRAFTADESSGMDPAIGGSYLGFIEKIPHLQDLGINAVELLPVFEFDELEL 300

Query: 302 QRRSNPRDHMINTWGYSTINFFAPMSRYASAGGGPLNASQEFKEMVKALHAAGIEVILDV 361
           QRRSNPRDHM+NTWGYST+NFFAPMSRYAS  G P+ AS+EFKEMVKALH+AGIEVILDV
Sbjct: 301 QRRSNPRDHMVNTWGYSTVNFFAPMSRYASGEGDPIKASKEFKEMVKALHSAGIEVILDV 360

Query: 362 VYNHTNEADDANPYTTSFRGIDNKVYYMLDLKNNGQLLNFSGCGNTLNCNHPVVMELVLD 421
           VYNHTNEADD  PYTTSFRGIDNKVYYMLD   N QLLNFSGCGNTLNCNHPVVMEL+LD
Sbjct: 361 VYNHTNEADDKYPYTTSFRGIDNKVYYMLD--PNNQLLNFSGCGNTLNCNHPVVMELILD 420

Query: 422 SLRHWVVEYHVDGFRFDLASTLCRGTDGAPLSAPPLIREISKDAILSRCKIIAEPWDCGG 481
           SLRHWV EYHVDGFRFDLAS LCR TDG+PLSAPPLIR I+KD++LSRCKIIAEPWDCGG
Sbjct: 421 SLRHWVTEYHVDGFRFDLASVLCRATDGSPLSAPPLIRAIAKDSVLSRCKIIAEPWDCGG 480

Query: 482 LYLVGRFPNWDRWAEWNGIYRDDVRKFIKGDCGMKGSFATRVAGSSDLYKVNKRKPYHGI 541
           LYLVG+FPNWDRWAEWNG+YRDDVR+FIKGD GMKGSFATRV+GSSDLY+VN+RKPYHG+
Sbjct: 481 LYLVGKFPNWDRWAEWNGMYRDDVRRFIKGDSGMKGSFATRVSGSSDLYQVNQRKPYHGV 540

Query: 542 NFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGSNDNFSWNCGFEGETEDTSIKALRSRQM 601
           NFVIAHDGFTLRDLVSYN KHN+ANGEGGNDG NDN SWNCGFEGET D  IK+LR+RQM
Sbjct: 541 NFVIAHDGFTLRDLVSYNFKHNEANGEGGNDGCNDNHSWNCGFEGETGDAHIKSLRTRQM 600

Query: 602 KNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDTAINHFQWGQLEARKRDHFRFFSE 661
           KNFHLALM SQGTPMMLMGDEYGHTRYGNNNSYGHDT++N+FQW +L+A+K++HFRFFSE
Sbjct: 601 KNFHLALMISQGTPMMLMGDEYGHTRYGNNNSYGHDTSLNNFQWKELDAKKQNHFRFFSE 660

Query: 662 VIKFRREHPQIFCRENFLHKDDITWHESNWDNPDSKFLAYTLHDD-NGEDVYLAFNAHHY 721
           VIKFR  H  +   ENFL + +ITWHE NWDN +SKFLA+TLHD   G D+Y+AFNAH Y
Sbjct: 661 VIKFRHSH-HVLKHENFLTQGEITWHEDNWDNSESKFLAFTLHDGIGGRDIYVAFNAHDY 720

Query: 722 YVDVSLPSPPTKRRWFRVVDTNLESPDDFVVDGIPSVGSTYNLAPYSSILLKAK 765
           +V   +P PP  ++WFRV DTNLESPDDFV +G+  V  TYN+AP+SSILL++K
Sbjct: 721 FVKALIPQPPPGKQWFRVADTNLESPDDFVREGVAGVADTYNVAPFSSILLQSK 764

BLAST of Moc11g06650 vs. TAIR 10
Match: AT2G39930.1 (isoamylase 1 )

HSP 1 Score: 600.9 bits (1548), Expect = 1.4e-171
Identity = 333/711 (46.84%), Postives = 429/711 (60.34%), Query Frame = 0

Query: 96  PLGVSEVDNGINFSIFSKHATSVTLCL-SLHERIDDRML-EFKLDPHVNRTGDVWHIRVQ 155
           P G +  D+G+NFS++S ++ S T+CL SL +   +++  E +LDP  NRTG VWH+ ++
Sbjct: 76  PFGPTVRDDGVNFSVYSTNSVSATICLISLSDLRQNKVTEEIQLDPSRNRTGHVWHVFLR 135

Query: 156 DLPRRNVLYGYRIDGPQGWHHGHRFDDRIVLLDPYAKFVEGRRYFG----GENKASGFLG 215
               +++LYGYR DG      GH +D   +LLDPYAK +  R  FG     +N       
Sbjct: 136 G-DFKDMLYGYRFDGKFSPEEGHYYDSSNILLDPYAKAIISRDEFGVLGPDDNCWPQMAC 195

Query: 216 TYDFGSMPFDWGDDYKLPNILEKDLVIYEMNVRSFTADESSGLASSTRGSYLGVIEKIPH 275
                   FDW  D  L  + +KDLVIYEM+VR FT  ESS +     G+Y GV EK+ H
Sbjct: 196 MVPTREEEFDWEGDMHL-KLPQKDLVIYEMHVRGFTRHESSKI--EFPGTYQGVAEKLDH 255

Query: 276 LLELGVNAVELLPVFEFDELEFQRRSN-PRDHMINTWGYSTINFFAPMSRYASAGGGPL- 335
           L ELG+N +EL+P  EF+ELE+   +    DH +N WGYSTI FF+PM RYASA      
Sbjct: 256 LKELGINCIELMPCHEFNELEYYSYNTILGDHRVNFWGYSTIGFFSPMIRYASASSNNFA 315

Query: 336 -NASQEFKEMVKALHAAGIEVILDVVYNHTNEADDANPYTTSFRGIDNKVYYMLDLKNNG 395
             A  EFK +VK  H  GIEVI+DVV NHT E ++  P   SFRG+DN VYYML  K  G
Sbjct: 316 GRAINEFKILVKEAHKRGIEVIMDVVLNHTAEGNEKGP-IFSFRGVDNSVYYMLAPK--G 375

Query: 396 QLLNFSGCGNTLNCNHPVVMELVLDSLRHWVVEYHVDGFRFDLASTLCRG---------- 455
           +  N+SGCGNT NCNHPVV + +LD LR+WV E HVDGFRFDL S + R           
Sbjct: 376 EFYNYSGCGNTFNCNHPVVRQFILDCLRYWVTEMHVDGFRFDLGSIMSRSSSLWDAANVY 435

Query: 456 ---------TDGAPLSAPPLIREISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEW 515
                    T G P+S PP+I  IS D IL   K+IAE WD GGLY VG FP+W  W+EW
Sbjct: 436 GADVEGDLLTTGTPISCPPVIDMISNDPILRGVKLIAEAWDAGGLYQVGMFPHWGIWSEW 495

Query: 516 NGIYRDDVRKFIKGDCGMKGSFATRVAGSSDLYKVNKRKPYHGINFVIAHDGFTLRDLVS 575
           NG +RD VR+FIKG  G  G+FA  + GS +LY+   RKP+H INF+ AHDGFTL DLV+
Sbjct: 496 NGKFRDVVRQFIKGTDGFSGAFAECLCGSPNLYQ-GGRKPWHSINFICAHDGFTLADLVT 555

Query: 576 YNVKHNDANGEGGNDGSNDNFSWNCGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMM 635
           YN K+N ANGE  NDG N N+SWNCG EG+    S+K LR RQM+NF ++LM SQG PM+
Sbjct: 556 YNNKNNLANGEENNDGENHNYSWNCGEEGDFASISVKRLRKRQMRNFFVSLMVSQGVPMI 615

Query: 636 LMGDEYGHTRYGNNNSYGHDTAINHFQWGQLEARKRDHFRFFSEVIKFRREHPQIFCREN 695
            MGDEYGHT+ GNNN+Y HD  +N+F+W + E    D FRF   +IKFR E   +   + 
Sbjct: 616 YMGDEYGHTKGGNNNTYCHDNYMNYFRWDKKEEAHSDFFRFCRILIKFRDECESLGLND- 675

Query: 696 FLHKDDITWHE-----SNWDNPDSKFLAYTLHDDNGEDVYLAFNAHHYYVDVSLPSPPTK 755
           F     + WH       NW +  S+F+A++L D   +++Y+AFN  H    VSLP+ P  
Sbjct: 676 FPTAKRLQWHGLAPEIPNW-SETSRFVAFSLVDSVKKEIYVAFNTSHLATLVSLPNRP-G 735

Query: 756 RRWFRVVDTNLESPDDFVVDGIPS------------VGSTYNLAPYSSILL 762
            RW   VDT+  SP D +   +P               + Y +  YSSI+L
Sbjct: 736 YRWEPFVDTSKPSPYDCITPDLPERETAMKQYRHFLDANVYPMLSYSSIIL 775

BLAST of Moc11g06650 vs. TAIR 10
Match: AT1G03310.2 (debranching enzyme 1 )

HSP 1 Score: 375.2 bits (962), Expect = 1.3e-103
Identity = 254/706 (35.98%), Postives = 359/706 (50.85%), Query Frame = 0

Query: 92  GEAFPLGVSE--VDNGINFSIFSKHATSVTLCLSLHERIDDRMLEFKLDPHVNRTGDVWH 151
           G   PLG+S    D+  NFS FS+ +T+V LCL      D   LE  LDP+VNRTGDVWH
Sbjct: 230 GHPLPLGLSSGPDDDSWNFSFFSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWH 289

Query: 152 IRVQDLPRRNVLYGYRIDGPQGWHHGHRFDDRIVLLDPYAKFVEGRRYFGGENKASGFLG 211
             V D     V YGYR             +   ++LDPYA  V       G++ +  +LG
Sbjct: 290 ASV-DNTWDFVRYGYRCKETAHSKEDVDVEGEPIVLDPYATVV-------GKSVSQKYLG 349

Query: 212 TYDFGSMPFDWGDDYKLPNILEKDLVIYEMNVRSFTADESSGLASSTRGSYLGVIEKIPH 271
           +    S  FDWG+D   PNI  + L++Y +NV+ FT   SS L S+  G++ GV EK+ H
Sbjct: 350 SLS-KSPSFDWGEDVS-PNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSH 409

Query: 272 LLELGVNAVELLPVFEFDELEFQRRSNPRDHMINTWGYSTINFFAPMSRYASAGGGPLN- 331
           L  LG NAV L P+F F E     +  P         Y   +FF+PM  Y     GP N 
Sbjct: 410 LKTLGTNAVLLEPIFSFSE-----QKGP---------YFPFHFFSPMDIY-----GPSNS 469

Query: 332 ---ASQEFKEMVKALHAAGIEVILDVVYNHTNEADDANPYTTSFRGIDNKVYYMLDLKNN 391
              A    K MVK LH+ GIEV+L+VV+ HT ++        + RGID+  YY     N+
Sbjct: 470 LESAVNSMKVMVKKLHSEGIEVLLEVVFTHTADSG-------ALRGIDDSSYYYKGRAND 529

Query: 392 GQLLNFSGCGNTLNCNHPVVMELVLDSLRHWVVEYHVDGFRFDLASTLCRGTDGAPLSAP 451
               ++      LNCN+PVV +LVL+SLR+WV E+HVDGF F  AS+L RG  G  LS P
Sbjct: 530 LDSKSY------LNCNYPVVQQLVLESLRYWVTEFHVDGFCFINASSLLRGVHGEQLSRP 589

Query: 452 PLIREISKDAILSRCKIIAEPWD-CGGLYLVGRFPNWDRWAEWNGIYRDDVRKFIKGDCG 511
           PL+  I+ D +L+  K+IA+ WD    +    RFP+W RWAE N  Y  +VR F++G  G
Sbjct: 590 PLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPHWKRWAELNTRYCRNVRNFLRGR-G 649

Query: 512 MKGSFATRVAGSSDLYKVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGS 571
           +    ATR+ GS D++  + R P    N++  + G +L D+VS++          G + +
Sbjct: 650 VLSDLATRICGSGDVF-TDGRGPAFSFNYISRNSGLSLVDIVSFS----------GPELA 709

Query: 572 NDNFSWNCGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSY 631
           ++  SWNCG EG T  +++   R +Q++NF      S G P++ MGDE G +  G+    
Sbjct: 710 SE-LSWNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTRGSPLLE 769

Query: 632 GHDTAINHFQWGQL-EARKRDHFRFFSEVIKFRREHPQIFCRENFLHKDDITWHESN--- 691
                   F W  L  A      +F S +   R     +F R +FL  ++I W+ ++   
Sbjct: 770 SR----KPFDWNLLASAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQTT 829

Query: 692 --WDNPDSKFLAYTLHDDNGE---------------DVYLAFNAHHYYVDVSLPSPPTKR 751
             W++P SKFLA  +  ++ E               D+++ FNA  +   V LPS P   
Sbjct: 830 PKWEDPASKFLALEIKSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDGS 876

Query: 752 RWFRVVDTNLESPDDFVVDGIPSVGS-------TYNLAPYSSILLK 763
           +W R+VDT L  P  F V+G   V          Y + PYS  L +
Sbjct: 890 KWRRLVDTALPFPGFFSVEGETVVAEEPLQQLVVYEMKPYSCTLFE 876

BLAST of Moc11g06650 vs. TAIR 10
Match: AT1G03310.1 (debranching enzyme 1 )

HSP 1 Score: 375.2 bits (962), Expect = 1.3e-103
Identity = 254/706 (35.98%), Postives = 359/706 (50.85%), Query Frame = 0

Query: 92  GEAFPLGVSE--VDNGINFSIFSKHATSVTLCLSLHERIDDRMLEFKLDPHVNRTGDVWH 151
           G   PLG+S    D+  NFS FS+ +T+V LCL      D   LE  LDP+VNRTGDVWH
Sbjct: 230 GHPLPLGLSSGPDDDSWNFSFFSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWH 289

Query: 152 IRVQDLPRRNVLYGYRIDGPQGWHHGHRFDDRIVLLDPYAKFVEGRRYFGGENKASGFLG 211
             V D     V YGYR             +   ++LDPYA  V       G++ +  +LG
Sbjct: 290 ASV-DNTWDFVRYGYRCKETAHSKEDVDVEGEPIVLDPYATVV-------GKSVSQKYLG 349

Query: 212 TYDFGSMPFDWGDDYKLPNILEKDLVIYEMNVRSFTADESSGLASSTRGSYLGVIEKIPH 271
           +    S  FDWG+D   PNI  + L++Y +NV+ FT   SS L S+  G++ GV EK+ H
Sbjct: 350 SLS-KSPSFDWGEDVS-PNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSH 409

Query: 272 LLELGVNAVELLPVFEFDELEFQRRSNPRDHMINTWGYSTINFFAPMSRYASAGGGPLN- 331
           L  LG NAV L P+F F E     +  P         Y   +FF+PM  Y     GP N 
Sbjct: 410 LKTLGTNAVLLEPIFSFSE-----QKGP---------YFPFHFFSPMDIY-----GPSNS 469

Query: 332 ---ASQEFKEMVKALHAAGIEVILDVVYNHTNEADDANPYTTSFRGIDNKVYYMLDLKNN 391
              A    K MVK LH+ GIEV+L+VV+ HT ++        + RGID+  YY     N+
Sbjct: 470 LESAVNSMKVMVKKLHSEGIEVLLEVVFTHTADSG-------ALRGIDDSSYYYKGRAND 529

Query: 392 GQLLNFSGCGNTLNCNHPVVMELVLDSLRHWVVEYHVDGFRFDLASTLCRGTDGAPLSAP 451
               ++      LNCN+PVV +LVL+SLR+WV E+HVDGF F  AS+L RG  G  LS P
Sbjct: 530 LDSKSY------LNCNYPVVQQLVLESLRYWVTEFHVDGFCFINASSLLRGVHGEQLSRP 589

Query: 452 PLIREISKDAILSRCKIIAEPWD-CGGLYLVGRFPNWDRWAEWNGIYRDDVRKFIKGDCG 511
           PL+  I+ D +L+  K+IA+ WD    +    RFP+W RWAE N  Y  +VR F++G  G
Sbjct: 590 PLVEAIAFDPLLAETKLIADCWDPLEMMPKEVRFPHWKRWAELNTRYCRNVRNFLRGR-G 649

Query: 512 MKGSFATRVAGSSDLYKVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHNDANGEGGNDGS 571
           +    ATR+ GS D++  + R P    N++  + G +L D+VS++          G + +
Sbjct: 650 VLSDLATRICGSGDVF-TDGRGPAFSFNYISRNSGLSLVDIVSFS----------GPELA 709

Query: 572 NDNFSWNCGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSY 631
           ++  SWNCG EG T  +++   R +Q++NF      S G P++ MGDE G +  G+    
Sbjct: 710 SE-LSWNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTRGSPLLE 769

Query: 632 GHDTAINHFQWGQL-EARKRDHFRFFSEVIKFRREHPQIFCRENFLHKDDITWHESN--- 691
                   F W  L  A      +F S +   R     +F R +FL  ++I W+ ++   
Sbjct: 770 SR----KPFDWNLLASAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQTT 829

Query: 692 --WDNPDSKFLAYTLHDDNGE---------------DVYLAFNAHHYYVDVSLPSPPTKR 751
             W++P SKFLA  +  ++ E               D+++ FNA  +   V LPS P   
Sbjct: 830 PKWEDPASKFLALEIKSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDGS 876

Query: 752 RWFRVVDTNLESPDDFVVDGIPSVGS-------TYNLAPYSSILLK 763
           +W R+VDT L  P  F V+G   V          Y + PYS  L +
Sbjct: 890 KWRRLVDTALPFPGFFSVEGETVVAEEPLQQLVVYEMKPYSCTLFE 876

BLAST of Moc11g06650 vs. TAIR 10
Match: AT5G04360.1 (limit dextrinase )

HSP 1 Score: 113.2 bits (282), Expect = 9.1e-25
Identity = 80/276 (28.99%), Postives = 129/276 (46.74%), Query Frame = 0

Query: 233 DLVIYEMNVRSFTADESSGLASSTRGSYLGVIEK-------IPHLLELGVNAVELLPVFE 292
           D+ IYE++VR F+A++ + +    RG YL    K       +  L++ G+  + LLP F+
Sbjct: 332 DISIYELHVRDFSANDET-VEPENRGGYLAFTSKDSAGVKHLQKLVDAGLTHLHLLPTFQ 391

Query: 293 FDELEFQRR----------------SNPRDHMIN--------TWGYSTINFFAPMSRYAS 352
           F +++ ++                 S      I          WGY+ + +  P   YAS
Sbjct: 392 FGDVDDEKENWKSVDTSLLEGLRPDSTEAQARITEIQNDDGYNWGYNPVLWGVPKGSYAS 451

Query: 353 AGGGPLNASQEFKEMVKALHAAGIEVILDVVYNHTNEADDANPYTTSFRGIDNKVYYMLD 412
              GP     EF++MV+AL+  G+ V+LDVVYNH + +   +  +   + +    YY+  
Sbjct: 452 DPTGPCRII-EFRKMVQALNCTGLNVVLDVVYNHLHASGPHDKESVLDKIVPG--YYL-- 511

Query: 413 LKNNGQLLNFSGCGNTLNCNHPVVMELVLDSLRHWVVEYHVDGFRFDLASTLCRGTDGAP 472
            +N+   +  S C N     H +V  L+ D L +WVV Y VDGFRFDL   + + T    
Sbjct: 512 RRNSDGFIENSTCVNNTASEHYMVDRLIRDDLLNWVVNYKVDGFRFDLMGHIMKATIVNA 571

Query: 473 LSAPPLIREISKDAILSRCKIIAEPWDCGGLYLVGR 478
            SA   +R+ +     SR  +  E W+ G +   GR
Sbjct: 572 KSAIGSLRKETDGVDGSRIYLYGEGWNFGEVAENGR 601