Moc11g03230 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc11g03230
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	protein DCL homolog, chloroplastic-like
Location	chr11: 2062133 .. 2065044 (+)
RNA-Seq Expression	Moc11g03230
Synteny	Moc11g03230

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGCTTCTTTGCTACTCCGGGGGCATCCTCTTCTTCGGTTGGGGCTCAGACACCGCGGTCTATGCACTGGAATCGTACAGGTGACTCGCCGGTCTTGCTGTACTGCGACGGCGGCGCCTACTCCACCAGACGGCAACTTAACATCTGCTGAAAATATTACCTCAGTTTTGAGTTCCAGTGACCCACCCAAGTACTCAAGGTGGGATGAGCCTGATTATCGGAAGTGGAAGGACCAGGAAGAGGAAATGCTCAACGACATCGAGCCTATCATATCCCTCACAAAAGAGATCCTCCACTCAAATAGGTTTGTATTATCCTCCCAATTTCACCCGGAATGTTTATACTTTATATCATAGGCACTATGTGTTTCAAAGGGGTAGGTTTTTCTGTTCAATGGGGATAACGCTTTTTGTTAAATTTCACAGACAGACATATAAGAACATGTTTTAAGTCAAAACGTAGTTAGAAATTTTGAATGGTATCCAGTATTTTTTTCATGACAATATGATAATTACTTTCGTAATGCAACTCTCAGCTAGATCACTTTAATTGGATCACTTGATAAATAGCATAATGAATTTGATATTTACATATGTGACCCTATATGTTGAAACTTTCCTGCAACAACAGCAACTACGGATATGCTTCTAAATATACTATAGTAGAAAGAAAAATGAACATGGGAGTTAGGATTAACGCCAGTGTGTTACATACGACCTTTCTTAGTTTTCTTTGTGGATTTGGTATCTGACTGATATTTGGCCTCAGCAAAGAACATGGAGTGACTTTGAGTTTAATTCATTCCATCAGAGGAGCATACTTCTCCATTATGCGTTTGTGGTCAGCTACGTGCTTGTAACTGAAATTTTGAAATTGTGGATTTGGGATCTTACTGTCGTGCTTGTAATCTTATGCTTCTCTTTGAGCATTTTTGAGTGTGGATATATTCAACTTTCTAGCTCATTCTTGTTATTTTTTTACTAGGTATGTGGATGGAGAGCGATTGACATCTGTGGACGAGAGAATTGTGGTTAACAGGCTTCTTGCTCATCATCCACATGCCGAAGACAAAATTGGATGTGGACTTGAATCCATCATGGTATTATTTAATGTGCATTGAATGGTTCAGAGTTCTTACTGTGTCATATTGTCTTACATTACTTCAGCTGGTCAATCAAGTTTCAAATATGCAGTTTGCAAGTTTTTTTTTGTTTGGTTGTATAGAAGTAAAGTCAGATAAAGAAACAAATGACATTTGGTATATAGATATTTTCAAAATCTCAAAATGCAATTTGTGACCTCTTGCCTAATCTGTAAAGCCCATACCAGAATTAATTTCTAACAAATTCACCCCCGTAGGCGAGATGGACCTTGGAAAGTAGCTTATTCTTCATCCTTTTCCTTTCTTTTCTTTTTTGGGTAGGAAAATCTCATGTATCTTTACTTGAAGTCTAGGCATTTTAGCAGCTGATATTAGCATTGAGATGAAAAAGGATTACAGAGTTGGGGAAGAAACTTGAGATCCCTCCCTTAATTCTTTCAACTCCAAGCCACTTGGAATCCAACACCTCTCATTTTAAGTCAATAATTTCTGCAATTCCAAACTAGTTTTGGTTAACAATTCCAGCGCAAATTACAAAGGATAAATTAATTAATTCCGAAAGTTGCCCTACCAATCATTTGCTACATGGAGCGTAAAGATAAAAGGATGAAATTGTAAAATAAGTGCTGGGGTGACCAAGTAAAATGACAAAAATAAATGGTAGGGATTGGCAGAAGCTTCTGGCTCACGCTCCACATCAGAATCTTTATCATAATACTTGCCTCCCTCTATCCTCCAACTCCCATTATAACTATTGCTCTAACAAACTTCCCAGCTAATGACTAATTTGGCCCTAAGAACATTTGTAGCGCACCTTCTCAAGTTTCTGCCCGTGCAGGTCAAGTAATGGCCTCCAATTCACACCCAACTCTTTCCCAGTAAGGGGCAAATTTGAAGTGTGCTGGAATTCATCATGCAATATCATACTAAGAGCAACTCATACCGAACTAATATTGAAGCTTGTCTGATTAAGACTTTTTATGCTATGTTGTGCTTGCTTGTACATTGAAGATTTCTGTTGGCTATCATGCTCTTCCGCTGAAGTTCGTACAGTAGAGATATAACACAGTTATAAAGCATGTTCAATGGTCACAAGTTTATTTTGCTGGAACAGAACAAATATGCTGGTGTTGCAAAAGTAGCTTGCTGGAGTCTGGCTGTGTTTTTATGTTGCTCAACTATGATCTTGGACCACATTTGAGCTAGGTTGCTACATGAGGACAATACCTTGCATTATATAGAGTAACTTTGTGGCTCATGGCCTTGCTTTGGCAGTCCTCACCTTACCTTGGCTCTTTTTGTTGGTTGTCTTCTCTTCTTTGGATTGAGGACATTTCTGTCTGTTGGCCAGGCTCCCTGGTTTAACATATATTCCATTTTCAAAATAAAAATATAAAAAAATTGAAAGTTGCAAGATACCAATTTTCCTTTTTTTTTTTAAAATGACACTTTACCTTCGTTGTCTACCCATACAATTTTATCTATCCTGTTCTTTTTTCCCTTTCCCACCCACCTCTCATTTTAAGACGTGCAGAAGCTTGCTAATATTTTGTTTGTTAGAGTTATTTGATGTTAACCCAGCCCAAAATCATCATTTTTTACCTTTTAAATAATGTAAGCTATTTTCTGCTGTTGAAGGTCGATCGGCATCCCCAATTTCGGCACTCAAGATGCCTCTTTGTTATAAGGACTGATGGTGGATGGATTGACTTCTCGTATCAAAAGTGTCTTCGGGCATATATCCGAAATAAGTATCCATCATATGCAGAGCGGTTTATCCGGGAACATTTCAAACGTGGCAGTTGA

mRNA sequence

ATGGCTGCTTCTTTGCTACTCCGGGGGCATCCTCTTCTTCGGTTGGGGCTCAGACACCGCGGTCTATGCACTGGAATCGTACAGGTGACTCGCCGGTCTTGCTGTACTGCGACGGCGGCGCCTACTCCACCAGACGGCAACTTAACATCTGCTGAAAATATTACCTCAGTTTTGAGTTCCAGTGACCCACCCAAGTACTCAAGGTGGGATGAGCCTGATTATCGGAAGTGGAAGGACCAGGAAGAGGAAATGCTCAACGACATCGAGCCTATCATATCCCTCACAAAAGAGATCCTCCACTCAAATAGGTATGTGGATGGAGAGCGATTGACATCTGTGGACGAGAGAATTGTGGTTAACAGGCTTCTTGCTCATCATCCACATGCCGAAGACAAAATTGGATGTGGACTTGAATCCATCATGGTCGATCGGCATCCCCAATTTCGGCACTCAAGATGCCTCTTTGTTATAAGGACTGATGGTGGATGGATTGACTTCTCGTATCAAAAGTGTCTTCGGGCATATATCCGAAATAAGTATCCATCATATGCAGAGCGGTTTATCCGGGAACATTTCAAACGTGGCAGTTGA

Coding sequence (CDS)

ATGGCTGCTTCTTTGCTACTCCGGGGGCATCCTCTTCTTCGGTTGGGGCTCAGACACCGCGGTCTATGCACTGGAATCGTACAGGTGACTCGCCGGTCTTGCTGTACTGCGACGGCGGCGCCTACTCCACCAGACGGCAACTTAACATCTGCTGAAAATATTACCTCAGTTTTGAGTTCCAGTGACCCACCCAAGTACTCAAGGTGGGATGAGCCTGATTATCGGAAGTGGAAGGACCAGGAAGAGGAAATGCTCAACGACATCGAGCCTATCATATCCCTCACAAAAGAGATCCTCCACTCAAATAGGTATGTGGATGGAGAGCGATTGACATCTGTGGACGAGAGAATTGTGGTTAACAGGCTTCTTGCTCATCATCCACATGCCGAAGACAAAATTGGATGTGGACTTGAATCCATCATGGTCGATCGGCATCCCCAATTTCGGCACTCAAGATGCCTCTTTGTTATAAGGACTGATGGTGGATGGATTGACTTCTCGTATCAAAAGTGTCTTCGGGCATATATCCGAAATAAGTATCCATCATATGCAGAGCGGTTTATCCGGGAACATTTCAAACGTGGCAGTTGA

Protein sequence

MAASLLLRGHPLLRLGLRHRGLCTGIVQVTRRSCCTATAAPTPPDGNLTSAENITSVLSSSDPPKYSRWDEPDYRKWKDQEEEMLNDIEPIISLTKEILHSNRYVDGERLTSVDERIVVNRLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKYPSYAERFIREHFKRGS

Homology

[Close]

BLAST of Moc11g03230 vs. NCBI nr
Match: XP_022135338.1 (protein DCL, chloroplastic isoform X1 [Momordica charantia])

HSP 1 Score: 406.4 bits (1043), Expect = 1.4e-109
Identity = 196/196 (100.00%), Postives = 196/196 (100.00%), Query Frame = 0


Query: 1   MAASLLLRGHPLLRLGLRHRGLCTGIVQVTRRSCCTATAAPTPPDGNLTSAENITSVLSS 60
           MAASLLLRGHPLLRLGLRHRGLCTGIVQVTRRSCCTATAAPTPPDGNLTSAENITSVLSS
Sbjct: 1   MAASLLLRGHPLLRLGLRHRGLCTGIVQVTRRSCCTATAAPTPPDGNLTSAENITSVLSS 60

Query: 61  SDPPKYSRWDEPDYRKWKDQEEEMLNDIEPIISLTKEILHSNRYVDGERLTSVDERIVVN 120
           SDPPKYSRWDEPDYRKWKDQEEEMLNDIEPIISLTKEILHSNRYVDGERLTSVDERIVVN
Sbjct: 61  SDPPKYSRWDEPDYRKWKDQEEEMLNDIEPIISLTKEILHSNRYVDGERLTSVDERIVVN 120

Query: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180
           RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY
Sbjct: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180

Query: 181 PSYAERFIREHFKRGS 197
           PSYAERFIREHFKRGS
Sbjct: 181 PSYAERFIREHFKRGS 196

[Close]

BLAST of Moc11g03230 vs. NCBI nr
Match: KAG6608449.1 (Protein DCL-like, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 362.1 bits (928), Expect = 3.1e-96
Identity = 172/196 (87.76%), Postives = 184/196 (93.88%), Query Frame = 0


Query: 1   MAASLLLRGHPLLRLGLRHRGLCTGIVQVTRRSCCTATAAPTPPDGNLTSAENITSVLSS 60
           M ASLLLRGHPL+RLGLR+RGLCTGIVQVTRRSCCTA  A TPP G L+SAEN TSVLS+
Sbjct: 1   MVASLLLRGHPLIRLGLRNRGLCTGIVQVTRRSCCTAMPASTPPGGGLSSAENTTSVLSA 60

Query: 61  SDPPKYSRWDEPDYRKWKDQEEEMLNDIEPIISLTKEILHSNRYVDGERLTSVDERIVVN 120
           +DPPKY RWDEP YRKWK+QEEE+L+DI+P+ISLTKEILHSNRYVDGERLT  DE+IVV+
Sbjct: 61  TDPPKYQRWDEPGYRKWKNQEEEILSDIDPVISLTKEILHSNRYVDGERLTFEDEKIVVD 120

Query: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180
           RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY
Sbjct: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180

Query: 181 PSYAERFIREHFKRGS 197
           P YAERFIREHFKRGS
Sbjct: 181 PLYAERFIREHFKRGS 196

[Close]

BLAST of Moc11g03230 vs. NCBI nr
Match: XP_022982288.1 (protein DCL homolog, chloroplastic-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 360.1 bits (923), Expect = 1.2e-95
Identity = 171/196 (87.24%), Postives = 184/196 (93.88%), Query Frame = 0


Query: 1   MAASLLLRGHPLLRLGLRHRGLCTGIVQVTRRSCCTATAAPTPPDGNLTSAENITSVLSS 60
           M ASLLLRGHPL+RLGLR+RGLCTGI+QVTRRSCCTA  A TPP G L+SAEN TSVLS+
Sbjct: 1   MVASLLLRGHPLIRLGLRNRGLCTGILQVTRRSCCTAMPASTPPGGGLSSAENTTSVLST 60

Query: 61  SDPPKYSRWDEPDYRKWKDQEEEMLNDIEPIISLTKEILHSNRYVDGERLTSVDERIVVN 120
           +DPPKY RWDEP YRKWK+QEEE+L+DI+PIISLTKEILHSNRYVDGERLT  DE+IVV+
Sbjct: 61  TDPPKYQRWDEPGYRKWKNQEEEILSDIDPIISLTKEILHSNRYVDGERLTFEDEKIVVD 120

Query: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180
           RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY
Sbjct: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180

Query: 181 PSYAERFIREHFKRGS 197
           P YA+RFIREHFKRGS
Sbjct: 181 PLYADRFIREHFKRGS 196

[Close]

BLAST of Moc11g03230 vs. NCBI nr
Match: XP_023536078.1 (protein DCL homolog, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 359.8 bits (922), Expect = 1.5e-95
Identity = 170/196 (86.73%), Postives = 183/196 (93.37%), Query Frame = 0


Query: 1   MAASLLLRGHPLLRLGLRHRGLCTGIVQVTRRSCCTATAAPTPPDGNLTSAENITSVLSS 60
           MAASLLLRGHPLLR GL HRGLC+G+VQVTRRSCCTATAA TPPDG+LTSAEN  SV S+
Sbjct: 1   MAASLLLRGHPLLRFGLWHRGLCSGVVQVTRRSCCTATAASTPPDGSLTSAENTASVSSA 60

Query: 61  SDPPKYSRWDEPDYRKWKDQEEEMLNDIEPIISLTKEILHSNRYVDGERLTSVDERIVVN 120
           S P KY RW EPDYRKWKDQE+E+L+D+EP+ISLTKEI+HSNRYVDGERLTS DERIVV+
Sbjct: 61  SQPTKYQRWCEPDYRKWKDQEDEILSDVEPVISLTKEIIHSNRYVDGERLTSEDERIVVD 120

Query: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180
           RLLAHHPHAEDKIGCGLE IMVDRHPQFRHSRCLFV+RTDGGWIDFSYQKCLRAYIR+KY
Sbjct: 121 RLLAHHPHAEDKIGCGLEFIMVDRHPQFRHSRCLFVVRTDGGWIDFSYQKCLRAYIRSKY 180

Query: 181 PSYAERFIREHFKRGS 197
           PSYAE FIREHFKRGS
Sbjct: 181 PSYAEMFIREHFKRGS 196

[Close]

BLAST of Moc11g03230 vs. NCBI nr
Match: XP_022940238.1 (protein DCL homolog, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 359.0 bits (920), Expect = 2.6e-95
Identity = 171/196 (87.24%), Postives = 183/196 (93.37%), Query Frame = 0


Query: 1   MAASLLLRGHPLLRLGLRHRGLCTGIVQVTRRSCCTATAAPTPPDGNLTSAENITSVLSS 60
           M ASLLLRGHPL+RLGLR+RGLC GIVQVTRRSCCTA  A TPP G L+SAEN TSVLS+
Sbjct: 1   MVASLLLRGHPLIRLGLRNRGLCAGIVQVTRRSCCTAVPASTPPGGGLSSAENTTSVLSA 60

Query: 61  SDPPKYSRWDEPDYRKWKDQEEEMLNDIEPIISLTKEILHSNRYVDGERLTSVDERIVVN 120
           +DPPKY RW+EP YRKWK+QEEE+L+DI+PIISLTKEILHSNRYVDGERLT  DE+IVV+
Sbjct: 61  TDPPKYQRWNEPGYRKWKNQEEEILSDIDPIISLTKEILHSNRYVDGERLTFEDEKIVVD 120

Query: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180
           RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY
Sbjct: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180

Query: 181 PSYAERFIREHFKRGS 197
           P YAERFIREHFKRGS
Sbjct: 181 PLYAERFIREHFKRGS 196

[Close]

BLAST of Moc11g03230 vs. ExPASy Swiss-Prot
Match: Q42463 (Protein DCL, chloroplastic OS=Solanum lycopersicum OX=4081 GN=DCL PE=2 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 4.9e-28
Identity = 54/122 (44.26%), Postives = 79/122 (64.75%), Query Frame = 0


Query: 73  DYRKWKDQEEEMLNDIEPIISLTKEILHSNRYVDGERLTSVDERIVVNRLLAHHPHAEDK 132
           D   W D E+++L D  P++   + ILHS +Y  G+RL+   +R ++ RLL +HP  + K
Sbjct: 98  DGEGWVDWEDQILEDTVPLVGFVRMILHSGKYAIGDRLSPDHQRTILQRLLPYHPECDKK 157

Query: 133 IGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKYPSYAERFIREHF 192
           IG G++ I V  HP F +SRCLF++R DG  +DFSY KC++  IR  YP YA+ FI  HF
Sbjct: 158 IGPGVDYITVGYHPDFENSRCLFIVRKDGETVDFSYWKCIKGLIRKNYPLYADSFILRHF 217

Query: 193 KR 195
           ++
Sbjct: 218 RK 219

[Close]

BLAST of Moc11g03230 vs. ExPASy Swiss-Prot
Match: Q9C642 (Protein DCL homolog, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DCL PE=2 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 1.9e-27
Identity = 54/135 (40.00%), Postives = 85/135 (62.96%), Query Frame = 0


Query: 60  SSDPPKYSRWDEPDYRKWKDQEEEMLNDIEPIISLTKEILHSNRYVDGERLTSVDERIVV 119
           SS+  +    +  +  ++ D E+++L    P++   + ILHS +Y + +RL+   ER ++
Sbjct: 80  SSEEEEEEEEENSEGDEFVDWEDKILEVTVPLVGFVRMILHSGKYANRDRLSPEHERTII 139

Query: 120 NRLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNK 179
             LL +HP  E KIGCG++ IMV  HP F  SRC+F++R DG  +DFSY KC++  I+ K
Sbjct: 140 EMLLPYHPECEKKIGCGIDYIMVGHHPDFESSRCMFIVRKDGEVVDFSYWKCIKGLIKKK 199

Query: 180 YPSYAERFIREHFKR 195
           YP YA+ FI  HF++
Sbjct: 200 YPLYADSFILRHFRK 214

[Close]

BLAST of Moc11g03230 vs. ExPASy Swiss-Prot
Match: Q5D869 (DNA-directed RNA polymerase V subunit 1 OS=Arabidopsis thaliana OX=3702 GN=NRPE1 PE=1 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 9.5e-24
Identity = 48/115 (41.74%), Postives = 76/115 (66.09%), Query Frame = 0


Query: 80   QEEEMLNDIEPIISLTKEILHSNRYVDGERLTSVDERIVVNRLLAHHPHAEDKIGCGLES 139
            +E+E+L+D+EP++   ++I+H + Y DG+ ++  D+  V+ ++L  HP  E K+G G++ 
Sbjct: 1735 EEQELLSDVEPVMRTLRKIMHPSAYPDGDPISDDDKTFVLEKILNFHPQKETKLGSGVDF 1794

Query: 140  IMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKYPSYAERFIREHFKR 195
            I VD+H  F  SRC FV+ TDG   DFSY+K L  Y+  KYP  AE FI ++F +
Sbjct: 1795 ITVDKHTIFSDSRCFFVVSTDGAKQDFSYRKSLNNYLMKKYPDRAEEFIDKYFTK 1849

[Close]

BLAST of Moc11g03230 vs. ExPASy Swiss-Prot
Match: Q9LQ02 (DNA-directed RNA polymerase IV subunit 1 OS=Arabidopsis thaliana OX=3702 GN=NRPD1 PE=1 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 2.4e-06
Identity = 37/110 (33.64%), Postives = 56/110 (50.91%), Query Frame = 0


Query: 87   DIEPIISLTKEILHSNRYVDGERLTSVDERIVVNRLLAHHPHAEDKIGCGLESIMVDRHP 146
            +IE +    K ILHS  Y   E L   DE + V  +L  HP++ +KIG G++ I V +  
Sbjct: 1338 NIELLSQSLKRILHS--YEINELLNERDEGL-VKMVLQLHPNSVEKIGPGVKGIRVAK-S 1397

Query: 147  QFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKYPSYAERFIREHFKRGS 197
            +   S C  V+R DG + DFSY KC+    +   P     +  ++ K G+
Sbjct: 1398 KHGDSCCFEVVRIDGTFEDFSYHKCVLGATKIIAPKKMNFYKSKYLKNGT 1443

[Close]

BLAST of Moc11g03230 vs. ExPASy TrEMBL
Match: A0A6J1C4J4 (protein DCL, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111007322 PE=4 SV=1)

HSP 1 Score: 406.4 bits (1043), Expect = 6.9e-110
Identity = 196/196 (100.00%), Postives = 196/196 (100.00%), Query Frame = 0


Query: 1   MAASLLLRGHPLLRLGLRHRGLCTGIVQVTRRSCCTATAAPTPPDGNLTSAENITSVLSS 60
           MAASLLLRGHPLLRLGLRHRGLCTGIVQVTRRSCCTATAAPTPPDGNLTSAENITSVLSS
Sbjct: 1   MAASLLLRGHPLLRLGLRHRGLCTGIVQVTRRSCCTATAAPTPPDGNLTSAENITSVLSS 60

Query: 61  SDPPKYSRWDEPDYRKWKDQEEEMLNDIEPIISLTKEILHSNRYVDGERLTSVDERIVVN 120
           SDPPKYSRWDEPDYRKWKDQEEEMLNDIEPIISLTKEILHSNRYVDGERLTSVDERIVVN
Sbjct: 61  SDPPKYSRWDEPDYRKWKDQEEEMLNDIEPIISLTKEILHSNRYVDGERLTSVDERIVVN 120

Query: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180
           RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY
Sbjct: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180

Query: 181 PSYAERFIREHFKRGS 197
           PSYAERFIREHFKRGS
Sbjct: 181 PSYAERFIREHFKRGS 196

[Close]

BLAST of Moc11g03230 vs. ExPASy TrEMBL
Match: A0A6J1J4G4 (protein DCL homolog, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481165 PE=4 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 5.7e-96
Identity = 171/196 (87.24%), Postives = 184/196 (93.88%), Query Frame = 0


Query: 1   MAASLLLRGHPLLRLGLRHRGLCTGIVQVTRRSCCTATAAPTPPDGNLTSAENITSVLSS 60
           M ASLLLRGHPL+RLGLR+RGLCTGI+QVTRRSCCTA  A TPP G L+SAEN TSVLS+
Sbjct: 1   MVASLLLRGHPLIRLGLRNRGLCTGILQVTRRSCCTAMPASTPPGGGLSSAENTTSVLST 60

Query: 61  SDPPKYSRWDEPDYRKWKDQEEEMLNDIEPIISLTKEILHSNRYVDGERLTSVDERIVVN 120
           +DPPKY RWDEP YRKWK+QEEE+L+DI+PIISLTKEILHSNRYVDGERLT  DE+IVV+
Sbjct: 61  TDPPKYQRWDEPGYRKWKNQEEEILSDIDPIISLTKEILHSNRYVDGERLTFEDEKIVVD 120

Query: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180
           RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY
Sbjct: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180

Query: 181 PSYAERFIREHFKRGS 197
           P YA+RFIREHFKRGS
Sbjct: 181 PLYADRFIREHFKRGS 196

[Close]

BLAST of Moc11g03230 vs. ExPASy TrEMBL
Match: A0A6J1FNQ4 (protein DCL homolog, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111445919 PE=4 SV=1)

HSP 1 Score: 359.0 bits (920), Expect = 1.3e-95
Identity = 171/196 (87.24%), Postives = 183/196 (93.37%), Query Frame = 0


Query: 1   MAASLLLRGHPLLRLGLRHRGLCTGIVQVTRRSCCTATAAPTPPDGNLTSAENITSVLSS 60
           M ASLLLRGHPL+RLGLR+RGLC GIVQVTRRSCCTA  A TPP G L+SAEN TSVLS+
Sbjct: 1   MVASLLLRGHPLIRLGLRNRGLCAGIVQVTRRSCCTAVPASTPPGGGLSSAENTTSVLSA 60

Query: 61  SDPPKYSRWDEPDYRKWKDQEEEMLNDIEPIISLTKEILHSNRYVDGERLTSVDERIVVN 120
           +DPPKY RW+EP YRKWK+QEEE+L+DI+PIISLTKEILHSNRYVDGERLT  DE+IVV+
Sbjct: 61  TDPPKYQRWNEPGYRKWKNQEEEILSDIDPIISLTKEILHSNRYVDGERLTFEDEKIVVD 120

Query: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180
           RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY
Sbjct: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180

Query: 181 PSYAERFIREHFKRGS 197
           P YAERFIREHFKRGS
Sbjct: 181 PLYAERFIREHFKRGS 196

[Close]

BLAST of Moc11g03230 vs. ExPASy TrEMBL
Match: A0A6J1IHJ1 (protein DCL homolog, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111477278 PE=4 SV=1)

HSP 1 Score: 354.0 bits (907), Expect = 4.1e-94
Identity = 168/196 (85.71%), Postives = 182/196 (92.86%), Query Frame = 0


Query: 1   MAASLLLRGHPLLRLGLRHRGLCTGIVQVTRRSCCTATAAPTPPDGNLTSAENITSVLSS 60
           MAASLLLRGHPLLR GL HRGLC+G+VQVTRRSCCTATAA TPPDG+LTSAEN  SV S+
Sbjct: 1   MAASLLLRGHPLLRFGLWHRGLCSGVVQVTRRSCCTATAASTPPDGSLTSAENTASVSSA 60

Query: 61  SDPPKYSRWDEPDYRKWKDQEEEMLNDIEPIISLTKEILHSNRYVDGERLTSVDERIVVN 120
           S   KY RW EPDYRKWKDQE+E+L+D+EP++SLTKEILHSNRYVDGERLTS DERIVV+
Sbjct: 61  SQSTKYQRWCEPDYRKWKDQEDEILSDVEPVMSLTKEILHSNRYVDGERLTSEDERIVVD 120

Query: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180
           RLLAHHPHAEDKIGCGLE IMVDRHPQFR+SRCLFV+RTDGGWIDFSYQKCLRAYIR+KY
Sbjct: 121 RLLAHHPHAEDKIGCGLEFIMVDRHPQFRYSRCLFVVRTDGGWIDFSYQKCLRAYIRSKY 180

Query: 181 PSYAERFIREHFKRGS 197
           PSYAE FIREHFKRGS
Sbjct: 181 PSYAEMFIREHFKRGS 196

[Close]

BLAST of Moc11g03230 vs. ExPASy TrEMBL
Match: A0A1S3BU88 (protein DCL, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103493574 PE=4 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 6.9e-94
Identity = 166/196 (84.69%), Postives = 180/196 (91.84%), Query Frame = 0


Query: 1   MAASLLLRGHPLLRLGLRHRGLCTGIVQVTRRSCCTATAAPTPPDGNLTSAENITSVLSS 60
           MAAS L RGHPLLRLGL+ RGLCTG+VQVTRRSCC+AT A TP DG+LT+ +N TS++SS
Sbjct: 1   MAASFLFRGHPLLRLGLKRRGLCTGVVQVTRRSCCSATVASTPSDGDLTAGDNATSLVSS 60

Query: 61  SDPPKYSRWDEPDYRKWKDQEEEMLNDIEPIISLTKEILHSNRYVDGERLTSVDERIVVN 120
           S+PPKY RWDEPDYRKWK+QEEE+L DIEPII LTKEILHS+RY DGERLT  DER VV+
Sbjct: 61  SEPPKYQRWDEPDYRKWKNQEEEILGDIEPIIFLTKEILHSHRYADGERLTLEDERTVVD 120

Query: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKY 180
           RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRC FVIRTDGGWIDFSYQKCLRAYIRNKY
Sbjct: 121 RLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCFFVIRTDGGWIDFSYQKCLRAYIRNKY 180

Query: 181 PSYAERFIREHFKRGS 197
           PS+AERFIREHFKRGS
Sbjct: 181 PSFAERFIREHFKRGS 196

[Close]

BLAST of Moc11g03230 vs. TAIR 10
Match: AT3G46630.1 (Protein of unknown function (DUF3223) )

HSP 1 Score: 231.5 bits (589), Expect = 5.9e-61
Identity = 122/210 (58.10%), Postives = 152/210 (72.38%), Query Frame = 0


Query: 1   MAASLLLRGHPLLRLG-LRHRG----LCTGIVQVTRRSCCTATAAP---------TPPDG 60
           M + LLLR  PLLR G L HR     +  GI+   RR  C+    P         +P +G
Sbjct: 1   MTSLLLLRTLPLLRNGFLNHRHQVGIVAGGILSHRRRLLCSLADRPQFYENNDSVSPVEG 60

Query: 61  NLTSAENITSVLSSSDPPKYSRWDEPDYRKWKDQEEEMLNDIEPIISLTKEILHSNRYVD 120
           +  +A N+TS +  +      R+++PDYRKWK+ E E+L DIEPI  L KEILHS+RY+D
Sbjct: 61  SGAAAMNVTSPVVDNS----WRYEDPDYRKWKNLEAEILRDIEPISLLAKEILHSDRYLD 120

Query: 121 GERLTSVDERIVVNRLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDF 180
           GERL   DE+IV+ +LL +HP+++DKIGCGL+ IMVDRHPQFRHSRCLFV+RTDGGWIDF
Sbjct: 121 GERLDFEDEKIVMEKLLPYHPYSKDKIGCGLDFIMVDRHPQFRHSRCLFVVRTDGGWIDF 180

Query: 181 SYQKCLRAYIRNKYPSYAERFIREHFKRGS 197
           SYQKCLRAY+R+KYPS+AERFIREHFKR S
Sbjct: 181 SYQKCLRAYVRDKYPSHAERFIREHFKRAS 206

[Close]

BLAST of Moc11g03230 vs. TAIR 10
Match: AT1G45230.1 (Protein of unknown function (DUF3223) )

HSP 1 Score: 124.0 bits (310), Expect = 1.3e-28
Identity = 54/135 (40.00%), Postives = 85/135 (62.96%), Query Frame = 0


Query: 60  SSDPPKYSRWDEPDYRKWKDQEEEMLNDIEPIISLTKEILHSNRYVDGERLTSVDERIVV 119
           SS+  +    +  +  ++ D E+++L    P++   + ILHS +Y + +RL+   ER ++
Sbjct: 80  SSEEEEEEEEENSEGDEFVDWEDKILEVTVPLVGFVRMILHSGKYANRDRLSPEHERTII 139

Query: 120 NRLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNK 179
             LL +HP  E KIGCG++ IMV  HP F  SRC+F++R DG  +DFSY KC++  I+ K
Sbjct: 140 EMLLPYHPECEKKIGCGIDYIMVGHHPDFESSRCMFIVRKDGEVVDFSYWKCIKGLIKKK 199

Query: 180 YPSYAERFIREHFKR 195
           YP YA+ FI  HF++
Sbjct: 200 YPLYADSFILRHFRK 214

[Close]

BLAST of Moc11g03230 vs. TAIR 10
Match: AT1G45230.2 (Protein of unknown function (DUF3223) )

HSP 1 Score: 122.9 bits (307), Expect = 2.9e-28
Identity = 54/135 (40.00%), Postives = 85/135 (62.96%), Query Frame = 0


Query: 60  SSDPPKYSRWDEPDYRKWKDQEEEMLNDIEPIISLTKEILHSNRYVDGERLTSVDERIVV 119
           SS+  +    +  +  ++ D E+++L    P++   + ILHS +Y + +RL+   ER ++
Sbjct: 80  SSEEEEEEEEENSEGDEFVDWEDKILEVTVPLVGFVRMILHSGKYANRDRLSPEHERTII 139

Query: 120 NRLLAHHPHAEDKIGCGLESIMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNK 179
             LL +HP  E KIGCG++ IMV  HP F  SRC+F++R DG  +DFSY KC++  I+ K
Sbjct: 140 EMLLPYHPECEKKIGCGIDYIMVWHHPDFESSRCMFIVRKDGEVVDFSYWKCIKGLIKKK 199

Query: 180 YPSYAERFIREHFKR 195
           YP YA+ FI  HF++
Sbjct: 200 YPLYADSFILRHFRK 214

[Close]

BLAST of Moc11g03230 vs. TAIR 10
Match: AT2G40030.1 (nuclear RNA polymerase D1B )

HSP 1 Score: 111.7 bits (278), Expect = 6.8e-25
Identity = 48/115 (41.74%), Postives = 76/115 (66.09%), Query Frame = 0


Query: 80   QEEEMLNDIEPIISLTKEILHSNRYVDGERLTSVDERIVVNRLLAHHPHAEDKIGCGLES 139
            +E+E+L+D+EP++   ++I+H + Y DG+ ++  D+  V+ ++L  HP  E K+G G++ 
Sbjct: 1735 EEQELLSDVEPVMRTLRKIMHPSAYPDGDPISDDDKTFVLEKILNFHPQKETKLGSGVDF 1794

Query: 140  IMVDRHPQFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKYPSYAERFIREHFKR 195
            I VD+H  F  SRC FV+ TDG   DFSY+K L  Y+  KYP  AE FI ++F +
Sbjct: 1795 ITVDKHTIFSDSRCFFVVSTDGAKQDFSYRKSLNNYLMKKYPDRAEEFIDKYFTK 1849

[Close]

BLAST of Moc11g03230 vs. TAIR 10
Match: AT1G63020.1 (nuclear RNA polymerase D1A )

HSP 1 Score: 53.9 bits (128), Expect = 1.7e-07
Identity = 37/110 (33.64%), Postives = 56/110 (50.91%), Query Frame = 0


Query: 87   DIEPIISLTKEILHSNRYVDGERLTSVDERIVVNRLLAHHPHAEDKIGCGLESIMVDRHP 146
            +IE +    K ILHS  Y   E L   DE + V  +L  HP++ +KIG G++ I V +  
Sbjct: 1338 NIELLSQSLKRILHS--YEINELLNERDEGL-VKMVLQLHPNSVEKIGPGVKGIRVAK-S 1397

Query: 147  QFRHSRCLFVIRTDGGWIDFSYQKCLRAYIRNKYPSYAERFIREHFKRGS 197
            +   S C  V+R DG + DFSY KC+    +   P     +  ++ K G+
Sbjct: 1398 KHGDSCCFEVVRIDGTFEDFSYHKCVLGATKIIAPKKMNFYKSKYLKNGT 1443

The following BLAST results are available for this feature:

BLAST of Moc11g03230 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022135338.1	1.4e-109	100.00	protein DCL, chloroplastic isoform X1 [Momordica charantia]	[more]
KAG6608449.1	3.1e-96	87.76	Protein DCL-like, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022982288.1	1.2e-95	87.24	protein DCL homolog, chloroplastic-like isoform X1 [Cucurbita maxima]	[more]
XP_023536078.1	1.5e-95	86.73	protein DCL homolog, chloroplastic-like [Cucurbita pepo subsp. pepo]	[more]
XP_022940238.1	2.6e-95	87.24	protein DCL homolog, chloroplastic-like [Cucurbita moschata]	[more]

BLAST of Moc11g03230 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q42463	4.9e-28	44.26	Protein DCL, chloroplastic OS=Solanum lycopersicum OX=4081 GN=DCL PE=2 SV=1	[more]
Q9C642	1.9e-27	40.00	Protein DCL homolog, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DCL PE=2 S...	[more]
Q5D869	9.5e-24	41.74	DNA-directed RNA polymerase V subunit 1 OS=Arabidopsis thaliana OX=3702 GN=NRPE1...	[more]
Q9LQ02	2.4e-06	33.64	DNA-directed RNA polymerase IV subunit 1 OS=Arabidopsis thaliana OX=3702 GN=NRPD...	[more]

BLAST of Moc11g03230 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C4J4	6.9e-110	100.00	protein DCL, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC11100...	[more]
A0A6J1J4G4	5.7e-96	87.24	protein DCL homolog, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 G...	[more]
A0A6J1FNQ4	1.3e-95	87.24	protein DCL homolog, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1IHJ1	4.1e-94	85.71	protein DCL homolog, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111477...	[more]
A0A1S3BU88	6.9e-94	84.69	protein DCL, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103493574 PE=4 SV=1	[more]

BLAST of Moc11g03230 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G46630.1	5.9e-61	58.10	Protein of unknown function (DUF3223)	[more]
AT1G45230.1	1.3e-28	40.00	Protein of unknown function (DUF3223)	[more]
AT1G45230.2	2.9e-28	40.00	Protein of unknown function (DUF3223)	[more]
AT2G40030.1	6.8e-25	41.74	nuclear RNA polymerase D1B	[more]
AT1G63020.1	1.7e-07	33.64	nuclear RNA polymerase D1A	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF11523	DUF3223	coord: 96..172 e-value: 3.0E-27 score: 95.1
None	No IPR available	GENE3D	3.10.450.40		coord: 81..178 e-value: 1.4E-23 score: 85.2
None	No IPR available	PANTHER	PTHR33415:SF4	DCL PROTEIN (DUF3223)	coord: 1..195
IPR044673	Protein DCL-like	PANTHER	PTHR33415	FAMILY NOT NAMED	coord: 1..195

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc11g03230.1	Moc11g03230.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009658	chloroplast organization
biological_process	GO:1901259	chloroplast rRNA processing
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0003729	mRNA binding