Moc11g02310 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc11g02310
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Cytochrome P450
Location	chr11: 1473633 .. 1475273 (-)
RNA-Seq Expression	Moc11g02310
Synteny	Moc11g02310

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGCCGTAGCCGGAATCTTCGCCTTTCTCTTCTTTCTCTACGCTCTCTTCAGGACAGCTCATCGGAAGAATCTCCCACCCGAAGCCGCCGGCGGTTGGCCGCTGATCGGCCACCTCCATCTACTAAGTTCAACAGAACCGACCCATATAACGTTCGCGAAAATGGCAGACACCTACGGCCCAATCTTTACGTTGAGAATGGGTATGCATAGGGCCCTGATAGTGAGCAATTGGGAAATAGCGAGAGAATGCTTTACTACCAACGACAGAGCATTTTCCTCCCGTCCAAAAATGGTGGCCTCGAAGCTACTCGGCTATGACTGTGCCATTTTTGGGTTGAGCCCCTACGGCCCACACTGGCGCCACGTCCGCAAAATCGCCACGCTCCAACTCCTAACCAGCCACCGCCTCGACCAGCTACAGCACATCAGAACATCGGAGGTTCAGCGTTCCATTAAGAAGCTGTATGAGTTATGCGTCAATAAAAGAGACCAAAAGGAAGTATTGGTGGATATGAAGACGTGGTTCGGAGATGTAACGTTGAACAACATATTTAGGATTGTGGTCGGAAAGAGATTCTCGACGGCCATGGGTGGCAGTGGTAATGAGGAGTACAGGAAGGCGTTGAGAGAATTCTTTGAATGGTTTGGAGTGTTCATTCCGTCGGATTTCTTCCCATTTCTGAGTTGGTTGGATTTGGGAGAGTATGAGAAGGCCATGAAGAAGACGGCAAAAATCCTGAACCATGTGTTTGATAAGTGGCTTGAAGAGCACCGGCAGAAGATAAATTCCAGTCATGTACTGGAGTCAGAGGAGCACGATTTTATGGACGTTATGCTTTCTTCTGTTAAAGATGATGAAGAAATTTCTAGCTACGATGATGATACTGTCATCAAATCTACATGTTTGGTATGCCTTTTTTTTTTATTAATTTGTTTCTCTTTTCAAAATTTTGAAACGAACTATAAAGAAAGGTTGATTTATCGTAGTGCTAAATTTAAACATTATTGTACAGACTCTCATGTTGGCCGGTTCTGACACTACAACAGTGGTAATGATATGGGCTCTTTCTTTACTTCTCAACAATCAAAAAGTACTCAAGAGAGCTCAACTTGAATTAGATGAACAAGTTGGGAGAGGAAGACAAGTGAAAGAGTCAGACATAGAGAAGCTTATATATCTCCAAGCAATTGTAAAAGAGACTTTGCGGTTGTATCCTCCAACACCACTAGCAATCCCTCACGAATCTATGGAAGATTGCACAATTGCTGGTTACCACATTCCAGCAGGAACACGACTCATGGTCAACATTCAAAAGCTTCAAAAAGACCCACTTGTATGGGAGGATCCTTGTGAATTTCGACCAGAAAGATTTCTGACAAGCCAAAAAGATTTCGACATTAGAGGGAAAAGCCCACAGTTGATACCATTTGGAAATGGGCGAAGGAGGTGCCCAGCAATCTATATGCCCTCCAAATATTGCATTTAACACTTGGAAATTTACTTCATGGATTTGATATTGATAGGCCATCTAAAGAACTACTCGATATGGATGAGAGTATTGGAATTACCACAACGAGAAGAAACTCACTCGAGGTGGTTTTAAGTCCACGCCTTCCCCCTCAAATTTACGAATGA

mRNA sequence

ATGGAAGCCGTAGCCGGAATCTTCGCCTTTCTCTTCTTTCTCTACGCTCTCTTCAGGACAGCTCATCGGAAGAATCTCCCACCCGAAGCCGCCGGCGGTTGGCCGCTGATCGGCCACCTCCATCTACTAAGTTCAACAGAACCGACCCATATAACGTTCGCGAAAATGGCAGACACCTACGGCCCAATCTTTACGTTGAGAATGGGTATGCATAGGGCCCTGATAGTGAGCAATTGGGAAATAGCGAGAGAATGCTTTACTACCAACGACAGAGCATTTTCCTCCCGTCCAAAAATGGTGGCCTCGAAGCTACTCGGCTATGACTGTGCCATTTTTGGGTTGAGCCCCTACGGCCCACACTGGCGCCACGTCCGCAAAATCGCCACGCTCCAACTCCTAACCAGCCACCGCCTCGACCAGCTACAGCACATCAGAACATCGGAGGTTCAGCGTTCCATTAAGAAGCTGTATGAGTTATGCGTCAATAAAAGAGACCAAAAGGAAGTATTGGTGGATATGAAGACGTGGTTCGGAGATGTAACGTTGAACAACATATTTAGGATTGTGGTCGGAAAGAGATTCTCGACGGCCATGGGTGGCAGTGGTAATGAGGAGTACAGGAAGGCGTTGAGAGAATTCTTTGAATGGTTTGGAGTGTTCATTCCGTCGGATTTCTTCCCATTTCTGAGTTGGTTGGATTTGGGAGAGTATGAGAAGGCCATGAAGAAGACGGCAAAAATCCTGAACCATGTGTTTGATAAGTGGCTTGAAGAGCACCGGCAGAAGATAAATTCCAGTCATGTACTGGAGTCAGAGGAGCACGATTTTATGGACGTTATGCTTTCTTCTGTTAAAGATGATGAAGAAATTTCTAGCTACGATGATGATACTGTCATCAAATCTACATGTTTGACTCTCATGTTGGCCGGTTCTGACACTACAACAGTGGTAATGATATGGGCTCTTTCTTTACTTCTCAACAATCAAAAAGTACTCAAGAGAGCTCAACTTGAATTAGATGAACAAGTTGGGAGAGGAAGACAAGTGAAAGAGTCAGACATAGAGAAGCTTATATATCTCCAAGCAATTGTAAAAGAGACTTTGCGGTTGTATCCTCCAACACCACTAGCAATCCCTCACGAATCTATGGAAGATTGCACAATTGCTGGTTACCACATTCCAGCAGGAACACGACTCATGGTCAACATTCAAAAGCTTCAAAAAGACCCACTTGTATGGGAGGATCCTTGTGAATTTCGACCAGAAAGATTTCTGACAAGCCAAAAAGATTTCGACATTAGAGGGAAAAGCCCACAGTTGATACCATTTGGAAATGGGCGAAGGAGGCCATCTAAAGAACTACTCGATATGGATGAGAGTATTGGAATTACCACAACGAGAAGAAACTCACTCGAGGTGGTTTTAAGTCCACGCCTTCCCCCTCAAATTTACGAATGA

Coding sequence (CDS)

ATGGAAGCCGTAGCCGGAATCTTCGCCTTTCTCTTCTTTCTCTACGCTCTCTTCAGGACAGCTCATCGGAAGAATCTCCCACCCGAAGCCGCCGGCGGTTGGCCGCTGATCGGCCACCTCCATCTACTAAGTTCAACAGAACCGACCCATATAACGTTCGCGAAAATGGCAGACACCTACGGCCCAATCTTTACGTTGAGAATGGGTATGCATAGGGCCCTGATAGTGAGCAATTGGGAAATAGCGAGAGAATGCTTTACTACCAACGACAGAGCATTTTCCTCCCGTCCAAAAATGGTGGCCTCGAAGCTACTCGGCTATGACTGTGCCATTTTTGGGTTGAGCCCCTACGGCCCACACTGGCGCCACGTCCGCAAAATCGCCACGCTCCAACTCCTAACCAGCCACCGCCTCGACCAGCTACAGCACATCAGAACATCGGAGGTTCAGCGTTCCATTAAGAAGCTGTATGAGTTATGCGTCAATAAAAGAGACCAAAAGGAAGTATTGGTGGATATGAAGACGTGGTTCGGAGATGTAACGTTGAACAACATATTTAGGATTGTGGTCGGAAAGAGATTCTCGACGGCCATGGGTGGCAGTGGTAATGAGGAGTACAGGAAGGCGTTGAGAGAATTCTTTGAATGGTTTGGAGTGTTCATTCCGTCGGATTTCTTCCCATTTCTGAGTTGGTTGGATTTGGGAGAGTATGAGAAGGCCATGAAGAAGACGGCAAAAATCCTGAACCATGTGTTTGATAAGTGGCTTGAAGAGCACCGGCAGAAGATAAATTCCAGTCATGTACTGGAGTCAGAGGAGCACGATTTTATGGACGTTATGCTTTCTTCTGTTAAAGATGATGAAGAAATTTCTAGCTACGATGATGATACTGTCATCAAATCTACATGTTTGACTCTCATGTTGGCCGGTTCTGACACTACAACAGTGGTAATGATATGGGCTCTTTCTTTACTTCTCAACAATCAAAAAGTACTCAAGAGAGCTCAACTTGAATTAGATGAACAAGTTGGGAGAGGAAGACAAGTGAAAGAGTCAGACATAGAGAAGCTTATATATCTCCAAGCAATTGTAAAAGAGACTTTGCGGTTGTATCCTCCAACACCACTAGCAATCCCTCACGAATCTATGGAAGATTGCACAATTGCTGGTTACCACATTCCAGCAGGAACACGACTCATGGTCAACATTCAAAAGCTTCAAAAAGACCCACTTGTATGGGAGGATCCTTGTGAATTTCGACCAGAAAGATTTCTGACAAGCCAAAAAGATTTCGACATTAGAGGGAAAAGCCCACAGTTGATACCATTTGGAAATGGGCGAAGGAGGCCATCTAAAGAACTACTCGATATGGATGAGAGTATTGGAATTACCACAACGAGAAGAAACTCACTCGAGGTGGTTTTAAGTCCACGCCTTCCCCCTCAAATTTACGAATGA

Protein sequence

MEAVAGIFAFLFFLYALFRTAHRKNLPPEAAGGWPLIGHLHLLSSTEPTHITFAKMADTYGPIFTLRMGMHRALIVSNWEIARECFTTNDRAFSSRPKMVASKLLGYDCAIFGLSPYGPHWRHVRKIATLQLLTSHRLDQLQHIRTSEVQRSIKKLYELCVNKRDQKEVLVDMKTWFGDVTLNNIFRIVVGKRFSTAMGGSGNEEYRKALREFFEWFGVFIPSDFFPFLSWLDLGEYEKAMKKTAKILNHVFDKWLEEHRQKINSSHVLESEEHDFMDVMLSSVKDDEEISSYDDDTVIKSTCLTLMLAGSDTTTVVMIWALSLLLNNQKVLKRAQLELDEQVGRGRQVKESDIEKLIYLQAIVKETLRLYPPTPLAIPHESMEDCTIAGYHIPAGTRLMVNIQKLQKDPLVWEDPCEFRPERFLTSQKDFDIRGKSPQLIPFGNGRRRPSKELLDMDESIGITTTRRNSLEVVLSPRLPPQIYE

Homology

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BLAST of Moc11g02310 vs. NCBI nr
Match: XP_038897198.1 (cytochrome P450 CYP82D47-like [Benincasa hispida])

HSP 1 Score: 731.1 bits (1886), Expect = 6.3e-207
Identity = 367/521 (70.44%), Postives = 420/521 (80.61%), Query Frame = 0


Query: 2   EAVAGIFAFLFFLYALFR------TAHRKNLPPEAAGGWPLIGHLHLLSSTEPTHITFAK 61
           + VAGIFA + FLYAL +        HRK LPPEA+G  PLIGHLHLL + EP H TFAK
Sbjct: 10  DTVAGIFAVIIFLYALLKIYGGRVRPHRKRLPPEASGALPLIGHLHLLDANEPAHTTFAK 69

Query: 62  MADTYGPIFTLRMGMHRALIVSNWEIARECFTTNDRAFSSRPKMVASKLLGYDCAIFGLS 121
           MAD YGPIFTLR+G +  LIVSNW+IARECFTTNDR F+SRPK+VASKLLGYD A+ GLS
Sbjct: 70  MADAYGPIFTLRLGAYTNLIVSNWKIARECFTTNDRIFASRPKLVASKLLGYDYAMLGLS 129

Query: 122 PYGPHWRHVRKIATLQLLTSHRLDQLQHIRTSEVQRSIKKLYELCVNKRD--QKEVLVDM 181
           PYGPHWRHVRKIA L+LLT+HRL++LQHIR SEVQ S+KKLYELCV  +D  +K+ LV+M
Sbjct: 130 PYGPHWRHVRKIAMLELLTNHRLEKLQHIRVSEVQTSMKKLYELCVRNKDNSEKKTLVEM 189

Query: 182 KTWFGDVTLNNIFRIVVGKRFSTAMGGSG--NEEYRKALREFFEWFGVFIPSDFFPFLSW 241
           KTWFGD+TLN IFRIVVGKRFSTA+  SG  NEEYRKALR+FFEWFGVF+PSD FPFL W
Sbjct: 190 KTWFGDITLNTIFRIVVGKRFSTAVDASGNDNEEYRKALRDFFEWFGVFVPSDSFPFLKW 249

Query: 242 LDLGEYEKAMKKTAKILNHVFDKWLEEHRQKINSSHVLESEEHDFMDVMLSSVKDDEEIS 301
           LDLG +EKAMKKTAK+L+ VFDKWL+EH+Q  N+      EEHDFMDVMLS VKDD ++S
Sbjct: 250 LDLGGHEKAMKKTAKMLDEVFDKWLQEHQQNRNNIGEENVEEHDFMDVMLSRVKDDGQLS 309

Query: 302 SYDDDTVIKSTCLTLMLAGSDTTTVVMIWALSLLLNNQKVLKRAQLELDEQVGRGRQVKE 361
            YD DTV K+TCL L+LAGSDTTTV MIWALSLLLNN +VLKRAQLELDE VGR RQV+E
Sbjct: 310 KYDADTVTKATCLALILAGSDTTTVTMIWALSLLLNNPEVLKRAQLELDEHVGRQRQVRE 369

Query: 362 SDIEKLIYLQAIVKETLRLYPPTPLAIPHESMEDCTIAGYHIPAGTRLMVNIQKLQKDPL 421
           SD+  L+YLQAIVKETLRLYP  P+ IPHES+EDC +AGYHIP GTRL+VNIQKLQKDP 
Sbjct: 370 SDVNNLLYLQAIVKETLRLYPAAPILIPHESIEDCMVAGYHIPKGTRLIVNIQKLQKDPE 429

Query: 422 VWEDPCEFRPERFLTSQKDFDIRGKSPQLIPFGNGRR----------------------- 481
           +W+DPCEFRPERFLTSQKDFD+RG+SPQLIPFG+GRR                       
Sbjct: 430 IWKDPCEFRPERFLTSQKDFDVRGQSPQLIPFGSGRRMCPGILFAMQVMHLTLANLLHGF 489

Query: 482 ---RPSKE-LLDMDESIGITTTRRNSLEVVLSPRLPPQIYE 486
              RPS E LLDM+ES G+T+ R+N LEVVL+PRLP Q+YE
Sbjct: 490 KIIRPSDELLLDMEESAGMTSIRKNPLEVVLTPRLPAQVYE 530

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BLAST of Moc11g02310 vs. NCBI nr
Match: XP_004147803.2 (cytochrome P450 CYP82D47 [Cucumis sativus] >KGN59914.1 hypothetical protein Csa_001231 [Cucumis sativus])

HSP 1 Score: 727.2 bits (1876), Expect = 9.0e-206
Identity = 358/522 (68.58%), Postives = 418/522 (80.08%), Query Frame = 0


Query: 2   EAVAGIFAFLFFLYALF-------RTAHRKNLPPEAAGGWPLIGHLHLLSSTEPTHITFA 61
           + VA IFA + FLYALF       R  HRK LPPE  G  PLIGHLHLL   EP HITFA
Sbjct: 16  DVVARIFALIIFLYALFKIYRGRARAHHRKRLPPEVGGALPLIGHLHLLDKNEPAHITFA 75

Query: 62  KMADTYGPIFTLRMGMHRALIVSNWEIARECFTTNDRAFSSRPKMVASKLLGYDCAIFGL 121
           KMAD YGPIFTLR+G++  LIVSNWEIARECFTTND+ F+SRPK+VASKLLGYD A+ GL
Sbjct: 76  KMADAYGPIFTLRLGLYTNLIVSNWEIARECFTTNDKIFASRPKLVASKLLGYDYAMLGL 135

Query: 122 SPYGPHWRHVRKIATLQLLTSHRLDQLQHIRTSEVQRSIKKLYELCV--NKRDQKEVLVD 181
           SPYGPHWRHVRK+  L+LLTSHRL +LQHIR SEVQ SIK LYELC+   K ++K  LV+
Sbjct: 136 SPYGPHWRHVRKLTMLELLTSHRLQKLQHIRVSEVQTSIKNLYELCLKNKKNNEKNALVE 195

Query: 182 MKTWFGDVTLNNIFRIVVGKRFSTAMGGSG---NEEYRKALREFFEWFGVFIPSDFFPFL 241
           MKTWFGD+TLN I RIVVGK+FSTA+  S    NEEYRKALR+FFEWFGVF+PSD FPFL
Sbjct: 196 MKTWFGDITLNTISRIVVGKQFSTAVDVSNTNENEEYRKALRDFFEWFGVFVPSDSFPFL 255

Query: 242 SWLDLGEYEKAMKKTAKILNHVFDKWLEEHRQKINSSHVLESEEHDFMDVMLSSVKDDEE 301
            WLDLG +EKAMKKTA++L+ VFDKW++EH+QK N+   ++ EEHDFMDVMLS+V+DD +
Sbjct: 256 KWLDLGGHEKAMKKTAQVLDEVFDKWIQEHQQKKNNLGAVKMEEHDFMDVMLSNVRDDGQ 315

Query: 302 ISSYDDDTVIKSTCLTLMLAGSDTTTVVMIWALSLLLNNQKVLKRAQLELDEQVGRGRQV 361
           +S YD   V K+TCL L+LAGSDTTTV MIWALSLLLNNQ+VLKRAQLELDE VGR RQV
Sbjct: 316 LSKYDAHIVTKATCLALILAGSDTTTVTMIWALSLLLNNQEVLKRAQLELDEHVGRQRQV 375

Query: 362 KESDIEKLIYLQAIVKETLRLYPPTPLAIPHESMEDCTIAGYHIPAGTRLMVNIQKLQKD 421
           KESD++ L+YLQA+VKETLRLYP  P+ IPHES+EDC +AGYHIP GTRL+VN+QKLQ+D
Sbjct: 376 KESDVKNLLYLQAVVKETLRLYPAAPILIPHESIEDCVVAGYHIPLGTRLIVNVQKLQRD 435

Query: 422 PLVWEDPCEFRPERFLTSQKDFDIRGKSPQLIPFGNGRR--------------------- 481
           P +WEDPCEF PERFLTS+KDFD+RG+SPQLIPFG+GRR                     
Sbjct: 436 PQIWEDPCEFHPERFLTSEKDFDVRGQSPQLIPFGSGRRMCPGISFALQVMHLALANLLH 495

Query: 482 -----RPSKELLDMDESIGITTTRRNSLEVVLSPRLPPQIYE 486
                RP+KELLDM+ES G+T+ R+N LEVVL+PRLPPQ+YE
Sbjct: 496 GFEISRPTKELLDMEESAGMTSIRKNPLEVVLTPRLPPQVYE 537

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BLAST of Moc11g02310 vs. NCBI nr
Match: XP_023535456.1 (cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 717.6 bits (1851), Expect = 7.2e-203
Identity = 352/519 (67.82%), Postives = 414/519 (79.77%), Query Frame = 0


Query: 2   EAVAGIFAFLFFLYALFRT------AHRKNLPPEAAGGWPLIGHLHLLSSTEPTHITFAK 61
           +  AGIFA + FLYAL +       AHRK LPPEA G  PLIGHLH L+  EP HI FAK
Sbjct: 10  DVFAGIFALIIFLYALSKIYGGTFGAHRKRLPPEAGGALPLIGHLHRLAGNEPAHIAFAK 69

Query: 62  MADTYGPIFTLRMGMHRALIVSNWEIARECFTTNDRAFSSRPKMVASKLLGYDCAIFGLS 121
           MAD YGPIFT+R+G++  LIVSNWEIARECF+TNDR F+SRPK+VASKLLGYD A+ GLS
Sbjct: 70  MADAYGPIFTVRLGVYTNLIVSNWEIARECFSTNDRIFASRPKLVASKLLGYDYAMMGLS 129

Query: 122 PYGPHWRHVRKIATLQLLTSHRLDQLQHIRTSEVQRSIKKLYELCVNKR---DQKEVLVD 181
           PYGPHWRHVRK+ATL+LLT+ RL+QLQHIR  EV+ SIKKLYE+CV  R     + VLV+
Sbjct: 130 PYGPHWRHVRKLATLELLTNQRLEQLQHIRVFEVRSSIKKLYEVCVKGRSNGSNEGVLVE 189

Query: 182 MKTWFGDVTLNNIFRIVVGKRFSTAMGGSGNEEYRKALREFFEWFGVFIPSDFFPFLSWL 241
           MKTWFGD+TLN IFRIVVGKRFSTA+ GSGNEEYR ALR+FFEWFGVF+PSD FPFL WL
Sbjct: 190 MKTWFGDITLNTIFRIVVGKRFSTAVEGSGNEEYRTALRDFFEWFGVFVPSDSFPFLKWL 249

Query: 242 DLGEYEKAMKKTAKILNHVFDKWLEEHRQKINSSHVLESEEHDFMDVMLSSVKDDEEISS 301
           DLG +EKAMK TAK+L+ VFDKWL+EHRQ  N S     E H FMDVMLS+VKDD ++S+
Sbjct: 250 DLGGHEKAMKNTAKVLDEVFDKWLQEHRQNKNVSEGKMEEPHHFMDVMLSNVKDDGQLSN 309

Query: 302 YDDDTVIKSTCLTLMLAGSDTTTVVMIWALSLLLNNQKVLKRAQLELDEQVGRGRQVKES 361
           YD  TV K+TCL L+LAGSDTTT+ M+WALSLLLN+ +V++RAQLELDE VGR RQV+ES
Sbjct: 310 YDAHTVTKATCLALILAGSDTTTITMVWALSLLLNHPEVIERAQLELDEHVGRQRQVRES 369

Query: 362 DIEKLIYLQAIVKETLRLYPPTPLAIPHESMEDCTIAGYHIPAGTRLMVNIQKLQKDPLV 421
           D++ L+YLQAIVKETLRLYP  P+ IPHES+EDCT+ GYHIPAGTRL+VN+QKLQ+DP +
Sbjct: 370 DVKNLLYLQAIVKETLRLYPAAPILIPHESIEDCTVVGYHIPAGTRLIVNVQKLQRDPQI 429

Query: 422 WEDPCEFRPERFLTSQKDFDIRGKSPQLIPFGNGR------------------------- 481
           W+DPCEFRPERFLT  KDFD+RG+SPQLIPFG+GR                         
Sbjct: 430 WKDPCEFRPERFLTDNKDFDVRGQSPQLIPFGSGRRMCPGISFALQVLHLTLANLLHGFE 489

Query: 482 -RRPSKELLDMDESIGITTTRRNSLEVVLSPRLPPQIYE 486
            RRPSKELLDM+ES G+T+ R+N LE V++PRLP Q+YE
Sbjct: 490 IRRPSKELLDMEESAGMTSIRKNLLEAVITPRLPAQVYE 528

[Close]

BLAST of Moc11g02310 vs. NCBI nr
Match: XP_022940489.1 (cytochrome P450 CYP82D47-like [Cucurbita moschata])

HSP 1 Score: 715.3 bits (1845), Expect = 3.5e-202
Identity = 357/508 (70.28%), Postives = 414/508 (81.50%), Query Frame = 0


Query: 3   AVAGIFAFLFFLYALFRTAHR-----KNLPPEAAGGWPLIGHLHLLSSTEPTHITFAKMA 62
           AVAGIFA L FLY LF  + R     K LPPE AG WP+IGHLHLLS+TEP HIT AKMA
Sbjct: 11  AVAGIFALLIFLYVLFNISKRLSSNPKRLPPEPAGAWPVIGHLHLLSTTEPAHITLAKMA 70

Query: 63  DTYGPIFTLRMGMHRALIVSNWEIARECFTTNDRAFSSRPKMVASKLLGYDCAIFGLSPY 122
           D YGPIFTLR+GMH+ALIVS+WEIARECFTT DR F+SRPK+VASKLLGY+ A+ GLSPY
Sbjct: 71  DVYGPIFTLRLGMHKALIVSSWEIARECFTTKDRIFASRPKLVASKLLGYNYAMMGLSPY 130

Query: 123 GPHWRHVRKIATLQLLTSHRLDQLQHIRTSEVQRSIKKLYELCVNKRDQKEVLVDMKTWF 182
           G HWRHVRK+ATL+L T+HRL QLQH+R SEV  SIKKLYELC N ++  + LVDM TWF
Sbjct: 131 GSHWRHVRKMATLELFTNHRLQQLQHVRVSEVDSSIKKLYELCGNGKE--KALVDMTTWF 190

Query: 183 GDVTLNNIFRIVVGKRFSTAMGGSGNEEYRKALREFFEWFGVFIPSDFFPFLSWLDLGEY 242
           GD+TLN IFR+VVGKRFSTA+ G GN+EYRKALREFFEWFGVF+PSD FPFL WLDLG +
Sbjct: 191 GDITLNTIFRVVVGKRFSTAVEGGGNDEYRKALREFFEWFGVFVPSDSFPFLKWLDLGGH 250

Query: 243 EKAMKKTAKILNHVFDKWLEEHRQKINSSHVLESEEHDFMDVMLSSVKDDEEISSYDDDT 302
           EKAMKKTA++L+ VFDKW+EE R+K+ +S   ++ EHDFMDVMLS+VKD +E+SSYD + 
Sbjct: 251 EKAMKKTAEVLDEVFDKWVEE-RRKMRASDEAKTAEHDFMDVMLSTVKDCDELSSYDANI 310

Query: 303 VIKSTCLTLMLAGSDTTTVVMIWALSLLLNNQKVLKRAQLELDEQVGRGRQVKESDIEKL 362
           VIKSTCL L+LAGSDTTTV MIWALSLLLNN + L++A+LELDEQVGR   VKESD++ L
Sbjct: 311 VIKSTCLALILAGSDTTTVTMIWALSLLLNNPEALEKARLELDEQVGRETHVKESDVKNL 370

Query: 363 IYLQAIVKETLRLYPPTPLAIPHESMEDCTIAGYHIPAGTRLMVNIQKLQKDPLVWEDPC 422
           IYLQAIVKETLRLYP  PL IPHES+EDCTI  YHIPAGTRLMVNIQKLQ+DP VWEDPC
Sbjct: 371 IYLQAIVKETLRLYPAGPLGIPHESIEDCTINDYHIPAGTRLMVNIQKLQRDPRVWEDPC 430

Query: 423 EFRPERFLTSQKDFDIRGKSPQLIPFGNGRR--------------------------RPS 480
           EFRPERFLT+ +DF++RG+SP+ IPFGNGRR                          RPS
Sbjct: 431 EFRPERFLTNHRDFNVRGQSPEFIPFGNGRRMCPGISFALQIMHLTLANLLHGFEIDRPS 490

[Close]

BLAST of Moc11g02310 vs. NCBI nr
Match: XP_008464765.1 (PREDICTED: cytochrome P450 CYP82D47-like [Cucumis melo] >KAA0032725.1 cytochrome P450 CYP82D47-like [Cucumis melo var. makuwa] >TYJ98756.1 cytochrome P450 CYP82D47-like [Cucumis melo var. makuwa])

HSP 1 Score: 714.9 bits (1844), Expect = 4.6e-202
Identity = 348/519 (67.05%), Postives = 419/519 (80.73%), Query Frame = 0


Query: 2   EAVAGIFAFLFFLYALFR-------TAHRKNLPPEAAGGWPLIGHLHLLSSTEPTHITFA 61
           + V GIFA + FLYALF+         HRK LPPE  G  PLIGHLHLL+  EP H TFA
Sbjct: 10  DTVTGIFALIIFLYALFKIYRGGVGARHRKRLPPEVGGALPLIGHLHLLAKNEPAHKTFA 69

Query: 62  KMADTYGPIFTLRMGMHRALIVSNWEIARECFTTNDRAFSSRPKMVASKLLGYDCAIFGL 121
           KMAD YGPIFTLR+G++  LIVSNWEIARECFTTND+ F+SRPK+VASKLLGYD A+FGL
Sbjct: 70  KMADAYGPIFTLRLGLYTNLIVSNWEIARECFTTNDKIFASRPKLVASKLLGYDYAMFGL 129

Query: 122 SPYGPHWRHVRKIATLQLLTSHRLDQLQHIRTSEVQRSIKKLYELCVNKRDQKEVLVDMK 181
           SPYGPHWRHVRK+A L+LL+++RL++LQHIR SEVQ S+K LYELC+  +++K  LV+MK
Sbjct: 130 SPYGPHWRHVRKLAMLELLSNYRLEKLQHIRVSEVQTSMKNLYELCL--KNKKNALVEMK 189

Query: 182 TWFGDVTLNNIFRIVVGKRFSTAMGGSG--NEEYRKALREFFEWFGVFIPSDFFPFLSWL 241
           TWFGD+TLN I RIVVGK+FSTA+  S   NEEYRKALR+FFEWFGVF+PSD FPFL WL
Sbjct: 190 TWFGDITLNTISRIVVGKKFSTAVDASNNENEEYRKALRDFFEWFGVFVPSDSFPFLKWL 249

Query: 242 DLGEYEKAMKKTAKILNHVFDKWLEEHRQKINSSHVLESEEHDFMDVMLSSVKDDEEISS 301
           DLG +EKAMKKTAK+L+ VFDKW+++H+  +     ++ EEHDFMDVMLS+V+DD ++S 
Sbjct: 250 DLGGHEKAMKKTAKVLDEVFDKWIQDHKNNLGE---VKMEEHDFMDVMLSNVRDDGQLSK 309

Query: 302 YDDDTVIKSTCLTLMLAGSDTTTVVMIWALSLLLNNQKVLKRAQLELDEQVGRGRQVKES 361
           YD  TV K+TCL+L+LAGSDTTTV MIWALSLLLNNQ+VLKRAQLELDE+VGR RQVKES
Sbjct: 310 YDAHTVTKATCLSLILAGSDTTTVTMIWALSLLLNNQEVLKRAQLELDERVGRQRQVKES 369

Query: 362 DIEKLIYLQAIVKETLRLYPPTPLAIPHESMEDCTIAGYHIPAGTRLMVNIQKLQKDPLV 421
           D++ L+YLQAIVKETLRLYP  P+ +PHES++DC +AGYHIP GTRL+VN+QKLQ+DP +
Sbjct: 370 DVKNLLYLQAIVKETLRLYPAAPILVPHESVDDCVVAGYHIPVGTRLIVNVQKLQRDPQI 429

Query: 422 WEDPCEFRPERFLTSQKDFDIRGKSPQLIPFGNGRR------------------------ 481
           WEDPCEFRPERFLTS KDFD+RG++P+LIPFG+GRR                        
Sbjct: 430 WEDPCEFRPERFLTSAKDFDVRGQNPRLIPFGSGRRMCPGISFALQVMHLALANLLHGFE 489

Query: 482 --RPSKELLDMDESIGITTTRRNSLEVVLSPRLPPQIYE 486
             RPSKE LDM+ES G+T+ R+N LEVVL+PRLPP +YE
Sbjct: 490 ISRPSKEFLDMEESAGMTSIRKNPLEVVLTPRLPPHVYE 523

[Close]

BLAST of Moc11g02310 vs. ExPASy Swiss-Prot
Match: O49858 (Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1)

HSP 1 Score: 458.8 bits (1179), Expect = 7.9e-128
Identity = 233/514 (45.33%), Postives = 330/514 (64.20%), Query Frame = 0


Query: 3   AVAGIFAFLFFLYALFRTAHRKNLPPEAAGGWPLIGHLHLLSSTEPTHITFAKMADTYGP 62
           A+A I + +F    L+R   R    P  +G WP++GHL LL+ ++  H     +AD YGP
Sbjct: 14  AIASILSLIFLCLFLYRKNSRGKDAPVVSGAWPILGHLSLLNGSQTPHKVLGALADKYGP 73

Query: 63  IFTLRMGMHRALIVSNWEIARECFTTNDRAFSSRPKMVASKLLGYDCAIFGLSPYGPHWR 122
           +FT+++GM  AL++SNWE+++E FTTND A SSRPK+VA +++ Y+ A  GL+PYGP+WR
Sbjct: 74  LFTIKLGMKPALVLSNWEMSKELFTTNDLAVSSRPKLVAVEVMSYNQAFVGLAPYGPYWR 133

Query: 123 HVRKIATLQLLTSHRLDQLQHIRTSEVQRSIKKLYELCV--NKRDQKEVLVDMKTWFGDV 182
            +RKI T + L++ R++Q  HIR SEV+ SIK+L+++    NK + +  LVD+K W   +
Sbjct: 134 ELRKIVTFEFLSNRRIEQRNHIRVSEVRTSIKELFDIWSNGNKNESRYTLVDIKQWLAYL 193

Query: 183 TLNNIFRIVVGKRFSTAMGGSGNEE---YRKALREFFEWFGVFIPSDFFPFLSWLDLGEY 242
           T N + R+VVGKR+   M   G ++   + K +REF    G F  +D  P L WLDLG +
Sbjct: 194 TFNMVVRMVVGKRYFGVMHVEGKDKAQRFMKNIREFMNLMGTFTVADGVPCLRWLDLGGH 253

Query: 243 EKAMKKTAKILNHVFDKWLEEHRQKINSSHVLESEEHDFMDVMLSSVKDDEEISSYDDDT 302
           EKAMK  AK ++ +  +WLEEHRQK      +ES + DFMDVM+S++ +  +I ++D DT
Sbjct: 254 EKAMKANAKEVDKLLSEWLEEHRQKKLLGENVES-DRDFMDVMISAL-NGAQIGAFDADT 313

Query: 303 VIKSTCLTLMLAGSDTTTVVMIWALSLLLNNQKVLKRAQLELDEQVGRGRQVKESDIEKL 362
           + K+T L L+L G+D+T V + WALSLLL N   L +A+ E+D Q+G+   ++ESDI KL
Sbjct: 314 ICKATSLELILGGTDSTAVTLTWALSLLLRNPLALGKAKEEIDMQIGKDEYIRESDISKL 373

Query: 363 IYLQAIVKETLRLYPPTPLAIPHESMEDCTIAGYHIPAGTRLMVNIQKLQKDPLVWEDPC 422
           +YLQAIVKETLRLYPP P + P E  E+C + GYHI  GTRL+ N+ K+ +DP VW DP 
Sbjct: 374 VYLQAIVKETLRLYPPAPFSSPREFTENCILGGYHIKKGTRLIHNLWKIHRDPSVWSDPL 433

Query: 423 EFRPERFLTSQKDFDIRGKSPQLIPFGNGRR--------------------------RPS 482
           EF+PERFLT+ KD D+RG + +L+PFG+GRR                           PS
Sbjct: 434 EFKPERFLTTHKDVDLRGHNFELLPFGSGRRVCAGMSLGLNMVHFTLANLLHSFDILNPS 493

Query: 483 KELLDMDESIGITTTRRNSLEVVLSPRLPPQIYE 486
            E +DM E  G T T+   LE+++ PR  P  YE
Sbjct: 494 AEPVDMTEFFGFTNTKATPLEILVKPRQSPNYYE 525

[Close]

BLAST of Moc11g02310 vs. ExPASy Swiss-Prot
Match: A0A0N7F297 (Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP82D61 PE=1 SV=1)

HSP 1 Score: 438.0 bits (1125), Expect = 1.4e-121
Identity = 235/512 (45.90%), Postives = 325/512 (63.48%), Query Frame = 0


Query: 6   GIFAFL--FFLYALFRTAHRKNLPPEAAGGWPLIGHLHLLSSTEPTHITFAKMADTYGPI 65
           G F  L  FF +   +  ++   PP+ AG WP+IGHLHLL+  +  H   +  AD  GP+
Sbjct: 13  GFFVLLPCFFYFVWKKPNNKIKEPPQPAGAWPIIGHLHLLARGDLPHKILSSFADKNGPV 72

Query: 66  FTLRMGMHRALIVSNWEIARECFTTNDRAFSSRPKMVASKLLGYDCAIFGLSPYGPHWRH 125
           F +++G+H+AL+V+N EIA+ECFTTNDR F +RP  VA+K++GY+  + G++PYGP+WR 
Sbjct: 73  FKIQLGVHQALVVNNSEIAKECFTTNDRFFLNRPSGVAAKIMGYNYVMLGVAPYGPYWRD 132

Query: 126 VRKIATLQLLTSHRLDQLQHIRTSEVQRSIKKLYELCVNKRDQKEVLVDMKTWFGDVTLN 185
           +RKI  L+ L++ RL  L+H+  SE+  S K+LY+L    ++    LVDMK W  D+TLN
Sbjct: 133 MRKIIMLEFLSNRRLQSLKHVWHSEISISSKELYKLW-ETQNIDFCLVDMKQWLADLTLN 192

Query: 186 NIFRIVVGKRFSTAMGGSGNEEYR-----KALREFFEWFGVFIPSDFFPFLSWLDLGEYE 245
              ++VVGKRF  +   S  EE       K LR  F   G F+ SD+ P+L WLDLG +E
Sbjct: 193 MSVKMVVGKRFFGSASASACEETESSNCPKTLRNMFRLMGSFVLSDYLPYLRWLDLGGHE 252

Query: 246 KAMKKTAKILNHVFDKWLEEHRQKINSSHVLESEEHDFMDVMLSSVKDDEEISSYDDDTV 305
           K MK+T K L+ +F  WL+EH++K   S   E ++ DFMDVMLS +  +E     D DT+
Sbjct: 253 KEMKRTVKELDILFKGWLDEHKRK-RLSGGKEDDDQDFMDVMLSIL--EESKLGNDVDTI 312

Query: 306 IKSTCLTLMLAGSDTTTVVMIWALSLLLNNQKVLKRAQLELDEQVGRGRQVKESDIEKLI 365
            K+ CL ++L G+DTT   + WALSLLLNN   LK+AQ ELD  VGR R V ESD+ KL 
Sbjct: 313 NKTACLAIILGGADTTWATLTWALSLLLNNPNALKKAQDELDLHVGRDRNVDESDLVKLT 372

Query: 366 YLQAIVKETLRLYPPTPLAIPHESMEDCTIAGYHIPAGTRLMVNIQKLQKDPLVWEDPCE 425
           Y+ AI+KETLRLYPP PL  P    EDCTIAGYH+ AGTRL+VN  K+Q+DPLVW  P E
Sbjct: 373 YIDAIIKETLRLYPPGPLLGPRVVTEDCTIAGYHVRAGTRLIVNAWKIQRDPLVWSQPHE 432

Query: 426 FRPERFLTSQKDFDIRGKSPQLIPFGNGR--------------------------RRPSK 485
           ++PERFL  ++D D++G+  +LIPFG+GR                          R P++
Sbjct: 433 YQPERFL--ERDVDMKGQHFELIPFGSGRRACPAISLALQVLPLTLAHILHGFELRTPNQ 492

[Close]

BLAST of Moc11g02310 vs. ExPASy Swiss-Prot
Match: Q43068 (Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2)

HSP 1 Score: 435.6 bits (1119), Expect = 7.1e-121
Identity = 237/533 (44.47%), Postives = 322/533 (60.41%), Query Frame = 0


Query: 3   AVAGIFAFLFFLYALFRTAHRKNLPPEAAGGWPLIGHLHLLSSTEPTHITFAKMADTYGP 62
           A   + + LFFL+   + +H K  PP  +G WPL+GHL L+ +T+  H T   + D YGP
Sbjct: 12  AFISLISLLFFLFRFSKVSHTKE-PPIISGSWPLLGHLPLMRNTQTPHKTLGALVDKYGP 71

Query: 63  IFTLRMGMHRALIVSNWEIARECFTTNDRAFSSRPKMVASKLLGYDCAIFGLSPYGPHWR 122
           IFT+++G   AL++SNWE+A+ECFT ND   SSRPK VA +L+ Y+ A  G +PYG +WR
Sbjct: 72  IFTIKLGATNALVLSNWELAKECFTKNDIVVSSRPKPVAVELMSYNQAFIGWAPYGAYWR 131

Query: 123 HVRKIATLQLLTSHRLDQLQHIRTSEVQRSIKKLYELCVNK--------RDQKE------ 182
            +RKI TL++L++ R++ L HIR SEVQ SIK+L  +  N+         D K       
Sbjct: 132 QLRKIVTLEILSNRRIELLSHIRVSEVQTSIKELVNVWSNQISSQYGLLDDTKSSSTNDE 191

Query: 183 ------VLVDMKTWFGDVTLNNIFRIVVGKRFSTAMGGSGNEEYRKAL---REFFEWFGV 242
                 V V++K WF  +TLN + R+VVGKR    +     EE ++ L   R+F    G 
Sbjct: 192 PSTTDYVSVELKKWFAQLTLNMVLRMVVGKRCFGDVDVENKEEAKRFLENIRDFMRLIGT 251

Query: 243 FIPSDFFPFLSWLDLGEYEKAMKKTAKILNHVFDKWLEEHRQKIN-SSHVLESEEHDFMD 302
           F   D  PFL WLDLG +EK MKK AK  + + ++WLEEHR+K    S      E DFMD
Sbjct: 252 FTVGDGVPFLKWLDLGGHEKEMKKCAKKFDVMLNEWLEEHREKKGLGSEDKVVGERDFMD 311

Query: 303 VMLSSVKDDEEISSYDDDTVIKSTCLTLMLAGSDTTTVVMIWALSLLLNNQKVLKRAQLE 362
            ML  +K D+ I  +D DT+IK+T L L+L GSDTT   + WA+ LLL +  VL++ + E
Sbjct: 312 AMLLVLK-DKPIEGFDVDTIIKATTLELILGGSDTTAGTLTWAMCLLLKHPHVLEKLKEE 371

Query: 363 LDEQVGRGRQVKESDIEKLIYLQAIVKETLRLYPPTPLAIPHESMEDCTIAGYHIPAGTR 422
           L+  +G+ R V ESDI KL+YL AI+KETLRLYPP P + P E  EDCTI GYHI  GTR
Sbjct: 372 LNTYIGKERCVNESDINKLVYLHAIIKETLRLYPPAPFSSPREFTEDCTIGGYHIKKGTR 431

Query: 423 LMVNIQKLQKDPLVWEDPCEFRPERFLTSQKDFDIRGKSPQLIPFGNGRR---------- 482
           LM N+ K+ +DP VW DP EF+PERFL++ KD D+RG++ +L+PFG+GRR          
Sbjct: 432 LMPNLWKIHRDPSVWPDPLEFKPERFLSTHKDVDVRGQNFELLPFGSGRRMCAGMSLGLH 491

Query: 483 ----------------RPSKELLDMDESIGITTTRRNSLEVVLSPRLPPQIYE 486
                            PS E +D+ E +   TT+   LEV++ P L  + YE
Sbjct: 492 MVHYILANFLHSFEILNPSPESIDVTEVLEFVTTKATPLEVLVKPCLSFKCYE 542

[Close]

BLAST of Moc11g02310 vs. ExPASy Swiss-Prot
Match: O49859 (Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1)

HSP 1 Score: 433.0 bits (1112), Expect = 4.6e-120
Identity = 229/511 (44.81%), Postives = 325/511 (63.60%), Query Frame = 0


Query: 4   VAGIFAFLFFLYALFRTAHRKNL-PPEAAGGWPLIGHLHLLSSTEPTHITFAKMADTYGP 63
           V+ IF    FLY   +  H  +   P   G WP+ GHL LL  ++  H     +A+ +GP
Sbjct: 16  VSLIFLLCLFLYGPLKKVHGSSKEAPTVGGAWPIFGHLPLLIGSKSPHKALGALAEKHGP 75

Query: 64  IFTLRMGMHRALIVSNWEIARECFTTNDRAFSSRPKMVASKLLGYDCAIFGLSPYGPHWR 123
           +FT+++G  +AL+VS+WE+ARECFTTND A S+RPK++ ++L+ Y+ A+  ++PYGP+WR
Sbjct: 76  LFTIKLGAKKALVVSDWEMARECFTTNDVAVSARPKLLVAELMCYNNAMLLVAPYGPYWR 135

Query: 124 HVRKIATLQLLTSHRLDQLQHIRTSEVQRSIKKLYELCVNKRDQKE-VLVDMKTWFGDVT 183
            +RKI   ++L+S R++QLQ +R SEVQ SI +LY++  +++++ +   V++K WF    
Sbjct: 136 ELRKIIVTEILSSSRVEQLQDVRVSEVQNSIVELYDVWRSQKNESDYASVELKQWFAQPI 195

Query: 184 LNNIFRIVVGKRFSTAMGGSGN-EEYRKALREFFEWFGVFIPSDFFPFLSWLDLGEYEKA 243
            N + R+VVGKRF +A       E+  KA+ EF    GVF   D  P+L WLD G YEKA
Sbjct: 196 FNMVLRMVVGKRFLSATATDEKAEKCVKAVDEFMRLAGVFTVGDAIPYLRWLDFGGYEKA 255

Query: 244 MKKTAKILNHVFDKWLEEHRQKINSSHVLESEEHDFMDVMLSSVKDDEEISSYDDDTVIK 303
           MK+TAK L+ +  +WLEEHRQK      ++  + DFM+VMLSS+ D + I   D DT+IK
Sbjct: 256 MKETAKELDVMISEWLEEHRQKRALGEGVDGAQ-DFMNVMLSSL-DGKTIDGIDADTLIK 315

Query: 304 STCLTLMLAGSDTTTVVMIWALSLLLNNQKVLKRAQLELDEQVGRGRQVKESDIEKLIYL 363
           ST LT++ AG++ +   +IWA+ L+L N  +L+  + ELD QVG+ R + ESDI  L+YL
Sbjct: 316 STVLTIIQAGTEASISTIIWAMCLILKNPLILEN-KAELDIQVGKDRCICESDISNLVYL 375

Query: 364 QAIVKETLRLYPPTPLAIPHESMEDCTIAGYHIPAGTRLMVNIQKLQKDPLVWEDPCEFR 423
           QA+VKETLRLY P PL+ P E  EDCT+ GYH+  GTRL+ NI K+  DP VW DP EF+
Sbjct: 376 QAVVKETLRLYAPGPLSSPREFAEDCTLGGYHVKKGTRLITNIWKIHTDPNVWSDPFEFK 435

Query: 424 PERFLTSQKDFDIRGKSPQLIPFGNGRR--------------------------RPSKEL 483
           P+RFLT+ KD D++G   QL+PFG+GRR                           PS E 
Sbjct: 436 PDRFLTTHKDIDVKGHHFQLLPFGSGRRVCPGISFGLQTVHLALASFLHSFEILNPSTEP 495

Query: 484 LDMDESIGITTTRRNSLEVVLSPRLPPQIYE 486
           LDM E+ G+T T+   LEV++ P L P  Y+
Sbjct: 496 LDMTEAFGVTNTKATPLEVLVKPCLSPSCYK 523

[Close]

BLAST of Moc11g02310 vs. ExPASy Swiss-Prot
Match: Q9SZ46 (Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=1 SV=1)

HSP 1 Score: 431.8 bits (1109), Expect = 1.0e-119
Identity = 217/449 (48.33%), Postives = 310/449 (69.04%), Query Frame = 0


Query: 11  LFFLYALFRTAHRKNL--PPEAAGGWPLIGHLHLLSSTEP-THITFAKMADTYGPIFTLR 70
           +F   ALF+ + +      P  +G WP+IGHLHLL   E   + T  KMAD YGP  +L+
Sbjct: 14  VFVFIALFKKSKKPKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLGKMADHYGPAMSLQ 73

Query: 71  MGMHRALIVSNWEIARECFTTNDRAFSSRPKMVASKLLGYDCAIFGLSPYGPHWRHVRKI 130
           +G + A +VS++E+A++CFT ND+A +SRP   A+K +GY+ A+FG +PY   WR +RKI
Sbjct: 74  LGSNEAFVVSSFEVAKDCFTVNDKALASRPMTAAAKHMGYNFAVFGFAPYSAFWREMRKI 133

Query: 131 ATLQLLTSHRLDQLQHIRTSEVQRSIKKLYELCVNKRDQKEVLVDMKTWFGDVTLNNIFR 190
           AT++LL++ RL  L+H+R SE+   +K LY L       K V+VD+K+W  D+TLN I R
Sbjct: 134 ATIELLSNRRLQMLKHVRVSEITMGVKDLYSLWFKNGGTKPVMVDLKSWLEDMTLNMIVR 193

Query: 191 IVVGKRFSTAMGGSGNE------EYRKALREFFEWFGVFIPSDFFPFLSWLDLGEYEKAM 250
           +V GKR+    G   +E      + +KA+ +FF   G+F  SD FP LS+ DL  +EK M
Sbjct: 194 MVAGKRYFGGGGSVSSEDTEEAMQCKKAIAKFFHLIGIFTVSDAFPTLSFFDLQGHEKEM 253

Query: 251 KKTAKILNHVFDKWLEEHRQKINSSHVLESEEHDFMDVMLSSVKDDE-EISSYDDDTVIK 310
           K+T   L+ + ++W+E HRQ+   S   E++  DF+DVM+S  +  +     YD +T IK
Sbjct: 254 KQTGSELDVILERWIENHRQQRKFSGTKENDS-DFIDVMMSLAEQGKLSHLQYDANTSIK 313

Query: 311 STCLTLMLAGSDTTTVVMIWALSLLLNNQKVLKRAQLELDEQVGRGRQVKESDIEKLIYL 370
           STCL L+L GSDT+   + WA+SLLLNN+++LK+AQ E+D  VGR R V++SDIE L+YL
Sbjct: 314 STCLALILGGSDTSASTLTWAISLLLNNKEMLKKAQDEIDIHVGRDRNVEDSDIENLVYL 373

Query: 371 QAIVKETLRLYPPTPLAIPHESMEDCTIAGYHIPAGTRLMVNIQKLQKDPLVWEDPCEFR 430
           QAI+KETLRLYP  PL  P E+MEDCT+AGY++P GTRL+VN+ K+Q+DP V+ +P EFR
Sbjct: 374 QAIIKETLRLYPAGPLLGPREAMEDCTVAGYYVPCGTRLIVNVWKIQRDPKVYMEPNEFR 433

Query: 431 PERFLTSQ-KDFDIRGKSPQLIPFGNGRR 449
           PERF+T + K+FD+RG++ +L+PFG+GRR
Sbjct: 434 PERFITGEAKEFDVRGQNFELMPFGSGRR 461

[Close]

BLAST of Moc11g02310 vs. ExPASy TrEMBL
Match: A0A0A0LG04 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G852630 PE=3 SV=1)

HSP 1 Score: 727.2 bits (1876), Expect = 4.4e-206
Identity = 358/522 (68.58%), Postives = 418/522 (80.08%), Query Frame = 0


Query: 2   EAVAGIFAFLFFLYALF-------RTAHRKNLPPEAAGGWPLIGHLHLLSSTEPTHITFA 61
           + VA IFA + FLYALF       R  HRK LPPE  G  PLIGHLHLL   EP HITFA
Sbjct: 16  DVVARIFALIIFLYALFKIYRGRARAHHRKRLPPEVGGALPLIGHLHLLDKNEPAHITFA 75

Query: 62  KMADTYGPIFTLRMGMHRALIVSNWEIARECFTTNDRAFSSRPKMVASKLLGYDCAIFGL 121
           KMAD YGPIFTLR+G++  LIVSNWEIARECFTTND+ F+SRPK+VASKLLGYD A+ GL
Sbjct: 76  KMADAYGPIFTLRLGLYTNLIVSNWEIARECFTTNDKIFASRPKLVASKLLGYDYAMLGL 135

Query: 122 SPYGPHWRHVRKIATLQLLTSHRLDQLQHIRTSEVQRSIKKLYELCV--NKRDQKEVLVD 181
           SPYGPHWRHVRK+  L+LLTSHRL +LQHIR SEVQ SIK LYELC+   K ++K  LV+
Sbjct: 136 SPYGPHWRHVRKLTMLELLTSHRLQKLQHIRVSEVQTSIKNLYELCLKNKKNNEKNALVE 195

Query: 182 MKTWFGDVTLNNIFRIVVGKRFSTAMGGSG---NEEYRKALREFFEWFGVFIPSDFFPFL 241
           MKTWFGD+TLN I RIVVGK+FSTA+  S    NEEYRKALR+FFEWFGVF+PSD FPFL
Sbjct: 196 MKTWFGDITLNTISRIVVGKQFSTAVDVSNTNENEEYRKALRDFFEWFGVFVPSDSFPFL 255

Query: 242 SWLDLGEYEKAMKKTAKILNHVFDKWLEEHRQKINSSHVLESEEHDFMDVMLSSVKDDEE 301
            WLDLG +EKAMKKTA++L+ VFDKW++EH+QK N+   ++ EEHDFMDVMLS+V+DD +
Sbjct: 256 KWLDLGGHEKAMKKTAQVLDEVFDKWIQEHQQKKNNLGAVKMEEHDFMDVMLSNVRDDGQ 315

Query: 302 ISSYDDDTVIKSTCLTLMLAGSDTTTVVMIWALSLLLNNQKVLKRAQLELDEQVGRGRQV 361
           +S YD   V K+TCL L+LAGSDTTTV MIWALSLLLNNQ+VLKRAQLELDE VGR RQV
Sbjct: 316 LSKYDAHIVTKATCLALILAGSDTTTVTMIWALSLLLNNQEVLKRAQLELDEHVGRQRQV 375

Query: 362 KESDIEKLIYLQAIVKETLRLYPPTPLAIPHESMEDCTIAGYHIPAGTRLMVNIQKLQKD 421
           KESD++ L+YLQA+VKETLRLYP  P+ IPHES+EDC +AGYHIP GTRL+VN+QKLQ+D
Sbjct: 376 KESDVKNLLYLQAVVKETLRLYPAAPILIPHESIEDCVVAGYHIPLGTRLIVNVQKLQRD 435

Query: 422 PLVWEDPCEFRPERFLTSQKDFDIRGKSPQLIPFGNGRR--------------------- 481
           P +WEDPCEF PERFLTS+KDFD+RG+SPQLIPFG+GRR                     
Sbjct: 436 PQIWEDPCEFHPERFLTSEKDFDVRGQSPQLIPFGSGRRMCPGISFALQVMHLALANLLH 495

Query: 482 -----RPSKELLDMDESIGITTTRRNSLEVVLSPRLPPQIYE 486
                RP+KELLDM+ES G+T+ R+N LEVVL+PRLPPQ+YE
Sbjct: 496 GFEISRPTKELLDMEESAGMTSIRKNPLEVVLTPRLPPQVYE 537

[Close]

BLAST of Moc11g02310 vs. ExPASy TrEMBL
Match: A0A6J1FKD2 (cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111446070 PE=3 SV=1)

HSP 1 Score: 715.3 bits (1845), Expect = 1.7e-202
Identity = 357/508 (70.28%), Postives = 414/508 (81.50%), Query Frame = 0


Query: 3   AVAGIFAFLFFLYALFRTAHR-----KNLPPEAAGGWPLIGHLHLLSSTEPTHITFAKMA 62
           AVAGIFA L FLY LF  + R     K LPPE AG WP+IGHLHLLS+TEP HIT AKMA
Sbjct: 11  AVAGIFALLIFLYVLFNISKRLSSNPKRLPPEPAGAWPVIGHLHLLSTTEPAHITLAKMA 70

Query: 63  DTYGPIFTLRMGMHRALIVSNWEIARECFTTNDRAFSSRPKMVASKLLGYDCAIFGLSPY 122
           D YGPIFTLR+GMH+ALIVS+WEIARECFTT DR F+SRPK+VASKLLGY+ A+ GLSPY
Sbjct: 71  DVYGPIFTLRLGMHKALIVSSWEIARECFTTKDRIFASRPKLVASKLLGYNYAMMGLSPY 130

Query: 123 GPHWRHVRKIATLQLLTSHRLDQLQHIRTSEVQRSIKKLYELCVNKRDQKEVLVDMKTWF 182
           G HWRHVRK+ATL+L T+HRL QLQH+R SEV  SIKKLYELC N ++  + LVDM TWF
Sbjct: 131 GSHWRHVRKMATLELFTNHRLQQLQHVRVSEVDSSIKKLYELCGNGKE--KALVDMTTWF 190

Query: 183 GDVTLNNIFRIVVGKRFSTAMGGSGNEEYRKALREFFEWFGVFIPSDFFPFLSWLDLGEY 242
           GD+TLN IFR+VVGKRFSTA+ G GN+EYRKALREFFEWFGVF+PSD FPFL WLDLG +
Sbjct: 191 GDITLNTIFRVVVGKRFSTAVEGGGNDEYRKALREFFEWFGVFVPSDSFPFLKWLDLGGH 250

Query: 243 EKAMKKTAKILNHVFDKWLEEHRQKINSSHVLESEEHDFMDVMLSSVKDDEEISSYDDDT 302
           EKAMKKTA++L+ VFDKW+EE R+K+ +S   ++ EHDFMDVMLS+VKD +E+SSYD + 
Sbjct: 251 EKAMKKTAEVLDEVFDKWVEE-RRKMRASDEAKTAEHDFMDVMLSTVKDCDELSSYDANI 310

Query: 303 VIKSTCLTLMLAGSDTTTVVMIWALSLLLNNQKVLKRAQLELDEQVGRGRQVKESDIEKL 362
           VIKSTCL L+LAGSDTTTV MIWALSLLLNN + L++A+LELDEQVGR   VKESD++ L
Sbjct: 311 VIKSTCLALILAGSDTTTVTMIWALSLLLNNPEALEKARLELDEQVGRETHVKESDVKNL 370

Query: 363 IYLQAIVKETLRLYPPTPLAIPHESMEDCTIAGYHIPAGTRLMVNIQKLQKDPLVWEDPC 422
           IYLQAIVKETLRLYP  PL IPHES+EDCTI  YHIPAGTRLMVNIQKLQ+DP VWEDPC
Sbjct: 371 IYLQAIVKETLRLYPAGPLGIPHESIEDCTINDYHIPAGTRLMVNIQKLQRDPRVWEDPC 430

Query: 423 EFRPERFLTSQKDFDIRGKSPQLIPFGNGRR--------------------------RPS 480
           EFRPERFLT+ +DF++RG+SP+ IPFGNGRR                          RPS
Sbjct: 431 EFRPERFLTNHRDFNVRGQSPEFIPFGNGRRMCPGISFALQIMHLTLANLLHGFEIDRPS 490

[Close]

BLAST of Moc11g02310 vs. ExPASy TrEMBL
Match: A0A5A7SU10 (Cytochrome P450 CYP82D47-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold156G00630 PE=3 SV=1)

HSP 1 Score: 714.9 bits (1844), Expect = 2.2e-202
Identity = 348/519 (67.05%), Postives = 419/519 (80.73%), Query Frame = 0


Query: 2   EAVAGIFAFLFFLYALFR-------TAHRKNLPPEAAGGWPLIGHLHLLSSTEPTHITFA 61
           + V GIFA + FLYALF+         HRK LPPE  G  PLIGHLHLL+  EP H TFA
Sbjct: 10  DTVTGIFALIIFLYALFKIYRGGVGARHRKRLPPEVGGALPLIGHLHLLAKNEPAHKTFA 69

Query: 62  KMADTYGPIFTLRMGMHRALIVSNWEIARECFTTNDRAFSSRPKMVASKLLGYDCAIFGL 121
           KMAD YGPIFTLR+G++  LIVSNWEIARECFTTND+ F+SRPK+VASKLLGYD A+FGL
Sbjct: 70  KMADAYGPIFTLRLGLYTNLIVSNWEIARECFTTNDKIFASRPKLVASKLLGYDYAMFGL 129

Query: 122 SPYGPHWRHVRKIATLQLLTSHRLDQLQHIRTSEVQRSIKKLYELCVNKRDQKEVLVDMK 181
           SPYGPHWRHVRK+A L+LL+++RL++LQHIR SEVQ S+K LYELC+  +++K  LV+MK
Sbjct: 130 SPYGPHWRHVRKLAMLELLSNYRLEKLQHIRVSEVQTSMKNLYELCL--KNKKNALVEMK 189

Query: 182 TWFGDVTLNNIFRIVVGKRFSTAMGGSG--NEEYRKALREFFEWFGVFIPSDFFPFLSWL 241
           TWFGD+TLN I RIVVGK+FSTA+  S   NEEYRKALR+FFEWFGVF+PSD FPFL WL
Sbjct: 190 TWFGDITLNTISRIVVGKKFSTAVDASNNENEEYRKALRDFFEWFGVFVPSDSFPFLKWL 249

Query: 242 DLGEYEKAMKKTAKILNHVFDKWLEEHRQKINSSHVLESEEHDFMDVMLSSVKDDEEISS 301
           DLG +EKAMKKTAK+L+ VFDKW+++H+  +     ++ EEHDFMDVMLS+V+DD ++S 
Sbjct: 250 DLGGHEKAMKKTAKVLDEVFDKWIQDHKNNLGE---VKMEEHDFMDVMLSNVRDDGQLSK 309

Query: 302 YDDDTVIKSTCLTLMLAGSDTTTVVMIWALSLLLNNQKVLKRAQLELDEQVGRGRQVKES 361
           YD  TV K+TCL+L+LAGSDTTTV MIWALSLLLNNQ+VLKRAQLELDE+VGR RQVKES
Sbjct: 310 YDAHTVTKATCLSLILAGSDTTTVTMIWALSLLLNNQEVLKRAQLELDERVGRQRQVKES 369

Query: 362 DIEKLIYLQAIVKETLRLYPPTPLAIPHESMEDCTIAGYHIPAGTRLMVNIQKLQKDPLV 421
           D++ L+YLQAIVKETLRLYP  P+ +PHES++DC +AGYHIP GTRL+VN+QKLQ+DP +
Sbjct: 370 DVKNLLYLQAIVKETLRLYPAAPILVPHESVDDCVVAGYHIPVGTRLIVNVQKLQRDPQI 429

Query: 422 WEDPCEFRPERFLTSQKDFDIRGKSPQLIPFGNGRR------------------------ 481
           WEDPCEFRPERFLTS KDFD+RG++P+LIPFG+GRR                        
Sbjct: 430 WEDPCEFRPERFLTSAKDFDVRGQNPRLIPFGSGRRMCPGISFALQVMHLALANLLHGFE 489

Query: 482 --RPSKELLDMDESIGITTTRRNSLEVVLSPRLPPQIYE 486
             RPSKE LDM+ES G+T+ R+N LEVVL+PRLPP +YE
Sbjct: 490 ISRPSKEFLDMEESAGMTSIRKNPLEVVLTPRLPPHVYE 523

[Close]

BLAST of Moc11g02310 vs. ExPASy TrEMBL
Match: A0A1S3CM78 (cytochrome P450 CYP82D47-like OS=Cucumis melo OX=3656 GN=LOC103502573 PE=3 SV=1)

HSP 1 Score: 714.9 bits (1844), Expect = 2.2e-202
Identity = 348/519 (67.05%), Postives = 419/519 (80.73%), Query Frame = 0


Query: 2   EAVAGIFAFLFFLYALFR-------TAHRKNLPPEAAGGWPLIGHLHLLSSTEPTHITFA 61
           + V GIFA + FLYALF+         HRK LPPE  G  PLIGHLHLL+  EP H TFA
Sbjct: 10  DTVTGIFALIIFLYALFKIYRGGVGARHRKRLPPEVGGALPLIGHLHLLAKNEPAHKTFA 69

Query: 62  KMADTYGPIFTLRMGMHRALIVSNWEIARECFTTNDRAFSSRPKMVASKLLGYDCAIFGL 121
           KMAD YGPIFTLR+G++  LIVSNWEIARECFTTND+ F+SRPK+VASKLLGYD A+FGL
Sbjct: 70  KMADAYGPIFTLRLGLYTNLIVSNWEIARECFTTNDKIFASRPKLVASKLLGYDYAMFGL 129

Query: 122 SPYGPHWRHVRKIATLQLLTSHRLDQLQHIRTSEVQRSIKKLYELCVNKRDQKEVLVDMK 181
           SPYGPHWRHVRK+A L+LL+++RL++LQHIR SEVQ S+K LYELC+  +++K  LV+MK
Sbjct: 130 SPYGPHWRHVRKLAMLELLSNYRLEKLQHIRVSEVQTSMKNLYELCL--KNKKNALVEMK 189

Query: 182 TWFGDVTLNNIFRIVVGKRFSTAMGGSG--NEEYRKALREFFEWFGVFIPSDFFPFLSWL 241
           TWFGD+TLN I RIVVGK+FSTA+  S   NEEYRKALR+FFEWFGVF+PSD FPFL WL
Sbjct: 190 TWFGDITLNTISRIVVGKKFSTAVDASNNENEEYRKALRDFFEWFGVFVPSDSFPFLKWL 249

Query: 242 DLGEYEKAMKKTAKILNHVFDKWLEEHRQKINSSHVLESEEHDFMDVMLSSVKDDEEISS 301
           DLG +EKAMKKTAK+L+ VFDKW+++H+  +     ++ EEHDFMDVMLS+V+DD ++S 
Sbjct: 250 DLGGHEKAMKKTAKVLDEVFDKWIQDHKNNLGE---VKMEEHDFMDVMLSNVRDDGQLSK 309

Query: 302 YDDDTVIKSTCLTLMLAGSDTTTVVMIWALSLLLNNQKVLKRAQLELDEQVGRGRQVKES 361
           YD  TV K+TCL+L+LAGSDTTTV MIWALSLLLNNQ+VLKRAQLELDE+VGR RQVKES
Sbjct: 310 YDAHTVTKATCLSLILAGSDTTTVTMIWALSLLLNNQEVLKRAQLELDERVGRQRQVKES 369

Query: 362 DIEKLIYLQAIVKETLRLYPPTPLAIPHESMEDCTIAGYHIPAGTRLMVNIQKLQKDPLV 421
           D++ L+YLQAIVKETLRLYP  P+ +PHES++DC +AGYHIP GTRL+VN+QKLQ+DP +
Sbjct: 370 DVKNLLYLQAIVKETLRLYPAAPILVPHESVDDCVVAGYHIPVGTRLIVNVQKLQRDPQI 429

Query: 422 WEDPCEFRPERFLTSQKDFDIRGKSPQLIPFGNGRR------------------------ 481
           WEDPCEFRPERFLTS KDFD+RG++P+LIPFG+GRR                        
Sbjct: 430 WEDPCEFRPERFLTSAKDFDVRGQNPRLIPFGSGRRMCPGISFALQVMHLALANLLHGFE 489

Query: 482 --RPSKELLDMDESIGITTTRRNSLEVVLSPRLPPQIYE 486
             RPSKE LDM+ES G+T+ R+N LEVVL+PRLPP +YE
Sbjct: 490 ISRPSKEFLDMEESAGMTSIRKNPLEVVLTPRLPPHVYE 523

[Close]

BLAST of Moc11g02310 vs. ExPASy TrEMBL
Match: A0A6J1J4B0 (cytochrome P450 CYP82D47-like OS=Cucurbita maxima OX=3661 GN=LOC111481116 PE=3 SV=1)

HSP 1 Score: 708.0 bits (1826), Expect = 2.7e-200
Identity = 353/508 (69.49%), Postives = 411/508 (80.91%), Query Frame = 0


Query: 3   AVAGIFAFLFFLYALFR-----TAHRKNLPPEAAGGWPLIGHLHLLSSTEPTHITFAKMA 62
           AVAGIF  L FLYALF      +++ K LPPE AG WP+IGHLHLLS+TEP HIT AKMA
Sbjct: 14  AVAGIFTLLIFLYALFNISTKLSSNPKRLPPEPAGAWPVIGHLHLLSTTEPAHITLAKMA 73

Query: 63  DTYGPIFTLRMGMHRALIVSNWEIARECFTTNDRAFSSRPKMVASKLLGYDCAIFGLSPY 122
           D YGPIFTL++GMHR LIVS+WEIARECFTT DR F+SRPK+VASKLLGY+ A+ GLSPY
Sbjct: 74  DVYGPIFTLKLGMHRTLIVSSWEIARECFTTKDRIFASRPKLVASKLLGYNYAMMGLSPY 133

Query: 123 GPHWRHVRKIATLQLLTSHRLDQLQHIRTSEVQRSIKKLYELCVNKRDQKEVLVDMKTWF 182
           G HWRHVRK+ATL+L T+HRL QLQH+R SEV+ SIKKLYELC N ++  + LVDMKTWF
Sbjct: 134 GSHWRHVRKMATLELFTNHRLQQLQHVRVSEVESSIKKLYELCGNGKE--KALVDMKTWF 193

Query: 183 GDVTLNNIFRIVVGKRFSTAMGGSGNEEYRKALREFFEWFGVFIPSDFFPFLSWLDLGEY 242
           GD+TLN IFR+VVGKRFSTA+ G GNEEYRKALREFFEWFGVF+PSD FPFL WLDLG +
Sbjct: 194 GDITLNTIFRVVVGKRFSTAVEGGGNEEYRKALREFFEWFGVFVPSDSFPFLKWLDLGGH 253

Query: 243 EKAMKKTAKILNHVFDKWLEEHRQKINSSHVLESEEHDFMDVMLSSVKDDEEISSYDDDT 302
           EKAMKKTA++L+ V DKW+EE R+K+ +S   ++ EHDFMDVMLS VKD EE+SSY+ +T
Sbjct: 254 EKAMKKTAEVLDEVLDKWVEE-RRKMRASDEAKTAEHDFMDVMLSGVKDYEELSSYEANT 313

Query: 303 VIKSTCLTLMLAGSDTTTVVMIWALSLLLNNQKVLKRAQLELDEQVGRGRQVKESDIEKL 362
           VIKSTCL L+LAGSDTTTV M+WALSLLLNN + LK+AQLELDE VGR   +KESD++ L
Sbjct: 314 VIKSTCLALILAGSDTTTVTMVWALSLLLNNPEALKKAQLELDEHVGRETHLKESDVKNL 373

Query: 363 IYLQAIVKETLRLYPPTPLAIPHESMEDCTIAGYHIPAGTRLMVNIQKLQKDPLVWEDPC 422
           +YLQAIVKETLRLYP  PL IPHES+EDCTI  YHIPAGTRLMVNIQKLQ+DP VWEDP 
Sbjct: 374 VYLQAIVKETLRLYPAGPLGIPHESIEDCTINDYHIPAGTRLMVNIQKLQRDPQVWEDPS 433

Query: 423 EFRPERFLTSQKDFDIRGKSPQLIPFGNGRR--------------------------RPS 480
           EFRPERFLTS +DF+ RG++P+ IPFGNGRR                          RPS
Sbjct: 434 EFRPERFLTSHRDFNFRGQTPEFIPFGNGRRMCPGISFALQIMHLTLANLLHEFEIDRPS 493

[Close]

BLAST of Moc11g02310 vs. TAIR 10
Match: AT4G31940.1 (cytochrome P450, family 82, subfamily C, polypeptide 4 )

HSP 1 Score: 431.8 bits (1109), Expect = 7.3e-121
Identity = 217/449 (48.33%), Postives = 310/449 (69.04%), Query Frame = 0


Query: 11  LFFLYALFRTAHRKNL--PPEAAGGWPLIGHLHLLSSTEP-THITFAKMADTYGPIFTLR 70
           +F   ALF+ + +      P  +G WP+IGHLHLL   E   + T  KMAD YGP  +L+
Sbjct: 14  VFVFIALFKKSKKPKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLGKMADHYGPAMSLQ 73

Query: 71  MGMHRALIVSNWEIARECFTTNDRAFSSRPKMVASKLLGYDCAIFGLSPYGPHWRHVRKI 130
           +G + A +VS++E+A++CFT ND+A +SRP   A+K +GY+ A+FG +PY   WR +RKI
Sbjct: 74  LGSNEAFVVSSFEVAKDCFTVNDKALASRPMTAAAKHMGYNFAVFGFAPYSAFWREMRKI 133

Query: 131 ATLQLLTSHRLDQLQHIRTSEVQRSIKKLYELCVNKRDQKEVLVDMKTWFGDVTLNNIFR 190
           AT++LL++ RL  L+H+R SE+   +K LY L       K V+VD+K+W  D+TLN I R
Sbjct: 134 ATIELLSNRRLQMLKHVRVSEITMGVKDLYSLWFKNGGTKPVMVDLKSWLEDMTLNMIVR 193

Query: 191 IVVGKRFSTAMGGSGNE------EYRKALREFFEWFGVFIPSDFFPFLSWLDLGEYEKAM 250
           +V GKR+    G   +E      + +KA+ +FF   G+F  SD FP LS+ DL  +EK M
Sbjct: 194 MVAGKRYFGGGGSVSSEDTEEAMQCKKAIAKFFHLIGIFTVSDAFPTLSFFDLQGHEKEM 253

Query: 251 KKTAKILNHVFDKWLEEHRQKINSSHVLESEEHDFMDVMLSSVKDDE-EISSYDDDTVIK 310
           K+T   L+ + ++W+E HRQ+   S   E++  DF+DVM+S  +  +     YD +T IK
Sbjct: 254 KQTGSELDVILERWIENHRQQRKFSGTKENDS-DFIDVMMSLAEQGKLSHLQYDANTSIK 313

Query: 311 STCLTLMLAGSDTTTVVMIWALSLLLNNQKVLKRAQLELDEQVGRGRQVKESDIEKLIYL 370
           STCL L+L GSDT+   + WA+SLLLNN+++LK+AQ E+D  VGR R V++SDIE L+YL
Sbjct: 314 STCLALILGGSDTSASTLTWAISLLLNNKEMLKKAQDEIDIHVGRDRNVEDSDIENLVYL 373

Query: 371 QAIVKETLRLYPPTPLAIPHESMEDCTIAGYHIPAGTRLMVNIQKLQKDPLVWEDPCEFR 430
           QAI+KETLRLYP  PL  P E+MEDCT+AGY++P GTRL+VN+ K+Q+DP V+ +P EFR
Sbjct: 374 QAIIKETLRLYPAGPLLGPREAMEDCTVAGYYVPCGTRLIVNVWKIQRDPKVYMEPNEFR 433

Query: 431 PERFLTSQ-KDFDIRGKSPQLIPFGNGRR 449
           PERF+T + K+FD+RG++ +L+PFG+GRR
Sbjct: 434 PERFITGEAKEFDVRGQNFELMPFGSGRR 461

[Close]

BLAST of Moc11g02310 vs. TAIR 10
Match: AT4G31970.1 (cytochrome P450, family 82, subfamily C, polypeptide 2 )

HSP 1 Score: 426.4 bits (1095), Expect = 3.1e-119
Identity = 228/512 (44.53%), Postives = 327/512 (63.87%), Query Frame = 0


Query: 11  LFFLYALFRTAHRKN--LPPEAAGGWPLIGHLHLLSSTEP-THITFAKMADTYGPIFTLR 70
           +F   ALF+ + +      P  +G WP+IGHLHLLS  E   + T  KMAD YGP  +LR
Sbjct: 14  VFVFIALFKKSKKPKHVKAPAPSGAWPIIGHLHLLSGKEQLLYRTLGKMADQYGPAMSLR 73

Query: 71  MGMHRALIVSNWEIARECFTTNDRAFSSRPKMVASKLLGYDCAIFGLSPYGPHWRHVRKI 130
           +G     +VS++E+A++CFT ND+A +SRP   A+K +GYDCA+FG +PY   WR +RKI
Sbjct: 74  LGSSETFVVSSFEVAKDCFTVNDKALASRPITAAAKHMGYDCAVFGFAPYSAFWREMRKI 133

Query: 131 ATLQLLTSHRLDQLQHIRTSEVQRSIKKLYELCVNKRDQKEVLVDMKTWFGDVTLNNIFR 190
           ATL+LL++ RL  L+H+R SE+   ++ LY L V K   + V+VD+K+W  D++LN + R
Sbjct: 134 ATLELLSNRRLQMLKHVRVSEISMVMQDLYSLWVKKGGSEPVMVDLKSWLEDMSLNMMVR 193

Query: 191 IVVGKRFSTAMGGSGNEE-------YRKALREFFEWFGVFIPSDFFPFLSWLDLGEYEKA 250
           +V GKR+    GGS + E        RK +  FF   G+F  SD FP L W D   +EK 
Sbjct: 194 MVAGKRYFG--GGSLSPEDAEEARQCRKGVANFFHLVGIFTVSDAFPKLGWFDFQGHEKE 253

Query: 251 MKKTAKILNHVFDKWLEEHRQKINSSHVLESEEHDFMDVMLSSVKDDE-EISSYDDDTVI 310
           MK+T + L+ + ++W+E HRQ+   S   +  + DF+DVMLS  +  +     +D  T I
Sbjct: 254 MKQTGRELDVILERWIENHRQQRKVSGT-KHNDSDFVDVMLSLAEQGKFSHLQHDAITSI 313

Query: 311 KSTCLTLMLAGSDTTTVVMIWALSLLLNNQKVLKRAQLELDEQVGRGRQVKESDIEKLIY 370
           KSTCL L+L GS+T+   + WA+SLLLNN+ +LK+AQ E+D  VGR R V++SDIE L+Y
Sbjct: 314 KSTCLALILGGSETSPSTLTWAISLLLNNKDMLKKAQDEIDIHVGRDRNVEDSDIENLVY 373

Query: 371 LQAIVKETLRLYPPTPLAIPHESMEDCTIAGYHIPAGTRLMVNIQKLQKDPLVWEDPCEF 430
           +QAI+KETLRLYP  PL    E++EDCT+AGY++  GTR++VN+ K+Q+DP V+ +P EF
Sbjct: 374 IQAIIKETLRLYPAGPLLGHREAIEDCTVAGYNVRRGTRMLVNVWKIQRDPRVYMEPNEF 433

Query: 431 RPERFLTSQ-KDFDIRGKSPQLIPFGNGRRR-PSKEL----------------------- 485
           RPERF+T + K+FD+RG++ +L+PFG+GRR  P   L                       
Sbjct: 434 RPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLQSFDVKTVMD 493

[Close]

BLAST of Moc11g02310 vs. TAIR 10
Match: AT4G31950.1 (cytochrome P450, family 82, subfamily C, polypeptide 3 )

HSP 1 Score: 396.0 bits (1016), Expect = 4.4e-110
Identity = 218/511 (42.66%), Postives = 318/511 (62.23%), Query Frame = 0


Query: 11  LFFLYALFRTAHRKNL--PPEAAGGWPLIGHLHLLSSTEP-THITFAKMADTYGPIFTLR 70
           +F   ALF+ + +      P  +G WP+IGHLHLL   E   + T  KMAD YGP  +LR
Sbjct: 14  VFVFIALFKKSKKPKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLGKMADHYGPAMSLR 73

Query: 71  MGMHRALIVSNWEIARECFTTNDRAFSSRPKMVASKLLGYDCAIFGLSPYGPHWRHVRKI 130
           +G     + S++E+A++CFT ND+A +S     A+K +GY   +F        W  +RKI
Sbjct: 74  LGSSETFVGSSFEVAKDCFTVNDKALASL-MTAAAKHMGY---VF--------WLEMRKI 133

Query: 131 ATLQLLTSHRLDQLQHIRTSEVQRSIKKLYELCVNKRDQKEVLVDMKTWFGDVTLNNIFR 190
           A ++LL++ RL  L ++R SE+   +K LY L V K   + V+VD+K+W  D+  N I R
Sbjct: 134 AMIELLSNRRLQMLNNVRVSEISMGVKDLYSLWVKKGGSEPVMVDLKSWLEDMIANMIMR 193

Query: 191 IVVGKRFSTAMGGSGNE------EYRKALREFFEWFGVFIPSDFFPFLSWLDLGEYEKAM 250
           +V GKR+    G   +E      ++RK + +FF   G+F  SD FP L WLDL  +EK M
Sbjct: 194 MVAGKRYFGGGGAESSEHTEEARQWRKGIAKFFHLVGIFTVSDAFPKLGWLDLQGHEKEM 253

Query: 251 KKTAKILNHVFDKWLEEHRQKINSSHVLESEEHDFMDVMLSSVKDDE-EISSYDDDTVIK 310
           K+T + L+ + ++W+E HRQ+   S   +  + DF+DVMLS  +  +     YD +T IK
Sbjct: 254 KQTRRELDVILERWIENHRQQRKVSGT-KHNDSDFVDVMLSLAEQGKLSHLQYDANTCIK 313

Query: 311 STCLTLMLAGSDTTTVVMIWALSLLLNNQKVLKRAQLELDEQVGRGRQVKESDIEKLIYL 370
           +TCL L+L GS+T+   + WA+SLLLNN+ +LK+ Q E+D  VGR R V++SDI+ L+YL
Sbjct: 314 TTCLALILGGSETSPSTLTWAISLLLNNKDMLKKVQDEIDIHVGRDRNVEDSDIKNLVYL 373

Query: 371 QAIVKETLRLYPPTPLAIPHESMEDCTIAGYHIPAGTRLMVNIQKLQKDPLVWEDPCEFR 430
           QAI+KETLRLYP  PL    E+MEDCT+AGY++P GTRL+VN+ K+Q+DP V+ +P EFR
Sbjct: 374 QAIIKETLRLYPAAPLLGHREAMEDCTVAGYNVPCGTRLIVNVWKIQRDPKVYMEPNEFR 433

Query: 431 PERFLTSQ-KDFDIRGKSPQLIPFGNGRRR---PSKEL---------------------- 485
           PERF+T + KDFD+RG++ +L+PFG+GRR    PS  +                      
Sbjct: 434 PERFITGEAKDFDVRGQNFELMPFGSGRRSCPGPSLAMQMLHLGLARFLHSFEVKTVLDR 493

[Close]

BLAST of Moc11g02310 vs. TAIR 10
Match: AT2G25160.1 (cytochrome P450, family 82, subfamily F, polypeptide 1 )

HSP 1 Score: 369.8 bits (948), Expect = 3.4e-102
Identity = 211/514 (41.05%), Postives = 306/514 (59.53%), Query Frame = 0


Query: 7   IFAFLFFLYALFRTAHRKNLPPEAAGGWPLIGHLHLLSSTEPTHITFAKMADTYGPIFTL 66
           IF  L  + +  R  ++K+  P   G WPL+GHLHL  +  PTH+TF  MAD YGP+F  
Sbjct: 14  IFPVLILIKSRLRPKNKKSTAPMVPGAWPLLGHLHLFDTVNPTHVTFGAMADVYGPVFMA 73

Query: 67  RMGMHRALIVSNWEIARECFTTNDRAFSSRPKMVASKLLGYDCAIFGLSPYGPHWRHVRK 126
           ++G  + +I+++ E+A+E +T +D+    RP++ ASKLLGY+ +    SPYG +WR +RK
Sbjct: 74  KLGSIKVMIINSKEVAKEIYTVHDKLL-ERPELTASKLLGYNDSFLTFSPYGLYWREIRK 133

Query: 127 IATLQLLTSHRLDQLQHIRTSEVQRSIKKLYELCVNKRDQKE-VLVDMKTWFGDVTLNNI 186
           IA  +L ++  +D     R  E   + + LY     +   KE VLVDMK  F D+T N  
Sbjct: 134 IAVSELFSTSGVDMHMVSRAREADLAFRALYVRWEKRGKPKEGVLVDMKQEFIDLTANIS 193

Query: 187 FRIVVGKRFSTAMGGSGNEEYRKA------LREFFEWFGVFIPSDFFPFLSWLDLGEYEK 246
             +V GKR+    G + N E ++A      +REF ++F +F+ SD  P L +LD  + ++
Sbjct: 194 LMMVSGKRY---FGENPNCEVKEARRCGKLIREFLDYFALFLLSDVAPVLGFLD-WKTKR 253

Query: 247 AMKKTAKILNHVFDKWLEEHRQKINSSHVLESEEHDFMDVMLSSVKDDEEISSYDDDTVI 306
            MK+TAK L+ V + W+EEH+ K  S H     E+D++D+++  +  D+     D  T I
Sbjct: 254 GMKRTAKGLDKVAEGWIEEHKNK-RSDH--GRSENDYLDILIKILGQDKIPGLSDTHTKI 313

Query: 307 KSTCLTLMLAGSDTTTVVMIWALSLLLNNQKVLKRAQLELDEQVGRGRQVKESDIEKLIY 366
           K+ CL L+LAGS+T  VV++WA+SLLLNN  VL++AQ ELD ++G+ R V+E DI+ L+Y
Sbjct: 314 KALCLNLVLAGSETAIVVLVWAVSLLLNNPHVLRKAQEELDSKIGKERVVEELDIKDLVY 373

Query: 367 LQAIVKETLRLYPPTPLAIPHESMEDCTIA--GYHIPAGTRLMVNIQKLQKDPLVWEDPC 426
           LQAIVKET RLYPP PL      +ED  IA    H+PAGT+LMV+  K+ +DP VW +P 
Sbjct: 374 LQAIVKETFRLYPPVPLVAYRAVVEDFDIAFCKCHVPAGTQLMVSAWKIHRDPNVWSNPE 433

Query: 427 EFRPERFLTSQKDFDIRGKSPQLIPFGNGRR--------------------------RPS 486
           +F PERFLTS ++ D+ G+S +  PFG GRR                          RPS
Sbjct: 434 QFEPERFLTSNRELDVGGQSYKFFPFGLGRRSCPAIPLGMRMVHYLLVRFLHSFDLARPS 493

[Close]

BLAST of Moc11g02310 vs. TAIR 10
Match: AT3G25180.1 (cytochrome P450, family 82, subfamily G, polypeptide 1 )

HSP 1 Score: 340.9 bits (873), Expect = 1.7e-93
Identity = 196/504 (38.89%), Postives = 285/504 (56.55%), Query Frame = 0


Query: 7   IFAFLFFLYALFRTAHRKNLPPEAAGGWPLIGHLHLLSSTEPTHITFAKMADTYGPIFTL 66
           IF ++F    L R     +  PE  G  PL GHLHLL   +      A M+  +GPIF+L
Sbjct: 19  IFGYIFLRKQLSRCEVDSSTIPEPLGALPLFGHLHLLRGKKLLCKKLAAMSQKHGPIFSL 78

Query: 67  RMGMHRALIVSNWEIARECFTTNDRAFSSRPKMVASKLLGYDCAIFGLSPYGPHWRHVRK 126
           ++G +R ++ S+ +  ++CFTTND A ++RP +   + +GY+ A   L+PYG +WR +RK
Sbjct: 79  KLGFYRLVVASDPKTVKDCFTTNDLATATRPNIAFGRYVGYNNASLTLAPYGDYWRELRK 138

Query: 127 IATLQLLTSHRLDQLQHIRTSEVQRSIKKLYELCVNKRDQKEVLVDMKTWFGDVTLNNIF 186
           I T+ L ++H ++ L HIR+SEV   IK LY     K +    +V +   F  +T N I 
Sbjct: 139 IVTVHLFSNHSIEMLGHIRSSEVNTLIKHLY-----KGNGGTSIVKIDMLFEFLTFNIIL 198

Query: 187 RIVVGKRFSTAMGGSGNEEYRKALREFFEWFGVFIPSDFFPFLSWLDLGEYEKAMKKTAK 246
           R +VGKR       S    Y++AL+       + +  D  P+L WLD  +    MK+  K
Sbjct: 199 RKMVGKRIGFGEVNSDEWRYKEALKHCEYLAVIPMIGDVIPWLGWLDFAK-NSQMKRLFK 258

Query: 247 ILNHVFDKWLEEHRQKINSSHVLESEEHDFMDVMLSSVKDDEEISSYDDDTVIKSTCLTL 306
            L+ V  KWL EH +K + +   + +E   MD++L  + +D  IS +  D ++K+T L L
Sbjct: 259 ELDSVNTKWLHEHLKKRSRNE--KDQERTIMDLLLDILPEDIVISGHVRDVIVKATILAL 318

Query: 307 MLAGSDTTTVVMIWALSLLLNNQKVLKRAQLELDEQVGRGRQVKESDIEKLIYLQAIVKE 366
            L GSD+T++ + WA+SLLLNN   L+ AQ E+D  VG+GR ++ESDI+ L YLQAIVKE
Sbjct: 319 TLTGSDSTSITLTWAVSLLLNNPAALEAAQEEIDNSVGKGRWIEESDIQNLKYLQAIVKE 378

Query: 367 TLRLYPPTPLAIPHESMEDCTIAGYHIPAGTRLMVNIQKLQKDPLVWEDPCEFRPERFLT 426
           T RLYPP PL    E+ EDC + GY +  GTRL+VNI KL +DP +W DP  F+PERF+ 
Sbjct: 379 THRLYPPAPLTGIREAREDCFVGGYRVEKGTRLLVNIWKLHRDPKIWPDPKTFKPERFME 438

Query: 427 SQKDFDIRGKSPQLIPFGNGRR--------------------------RPSKELLDMDES 485
            +   +    + + IPFG+GRR                          + S E LDM E 
Sbjct: 439 DKSQCE--KSNFEYIPFGSGRRSCPGVNLGLRVVHFVLARLLQGFELHKVSDEPLDMAEG 498

The following BLAST results are available for this feature:

BLAST of Moc11g02310 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038897198.1	6.3e-207	70.44	cytochrome P450 CYP82D47-like [Benincasa hispida]	[more]
XP_004147803.2	9.0e-206	68.58	cytochrome P450 CYP82D47 [Cucumis sativus] >KGN59914.1 hypothetical protein Csa_...	[more]
XP_023535456.1	7.2e-203	67.82	cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo]	[more]
XP_022940489.1	3.5e-202	70.28	cytochrome P450 CYP82D47-like [Cucurbita moschata]	[more]
XP_008464765.1	4.6e-202	67.05	PREDICTED: cytochrome P450 CYP82D47-like [Cucumis melo] >KAA0032725.1 cytochrome...	[more]

BLAST of Moc11g02310 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49858	7.9e-128	45.33	Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1	[more]
A0A0N7F297	1.4e-121	45.90	Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP...	[more]
Q43068	7.1e-121	44.47	Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2	[more]
O49859	4.6e-120	44.81	Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1	[more]
Q9SZ46	1.0e-119	48.33	Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=...	[more]

BLAST of Moc11g02310 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LG04	4.4e-206	68.58	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G852630 PE=3 SV=1	[more]
A0A6J1FKD2	1.7e-202	70.28	cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111446070 PE=3...	[more]
A0A5A7SU10	2.2e-202	67.05	Cytochrome P450 CYP82D47-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]
A0A1S3CM78	2.2e-202	67.05	cytochrome P450 CYP82D47-like OS=Cucumis melo OX=3656 GN=LOC103502573 PE=3 SV=1	[more]
A0A6J1J4B0	2.7e-200	69.49	cytochrome P450 CYP82D47-like OS=Cucurbita maxima OX=3661 GN=LOC111481116 PE=3 S...	[more]

BLAST of Moc11g02310 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G31940.1	7.3e-121	48.33	cytochrome P450, family 82, subfamily C, polypeptide 4	[more]
AT4G31970.1	3.1e-119	44.53	cytochrome P450, family 82, subfamily C, polypeptide 2	[more]
AT4G31950.1	4.4e-110	42.66	cytochrome P450, family 82, subfamily C, polypeptide 3	[more]
AT2G25160.1	3.4e-102	41.05	cytochrome P450, family 82, subfamily F, polypeptide 1	[more]
AT3G25180.1	1.7e-93	38.89	cytochrome P450, family 82, subfamily G, polypeptide 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 441..450 score: 47.77 coord: 309..326 score: 37.45 coord: 362..373 score: 47.01
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 32..448 e-value: 1.2E-89 score: 301.2
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 440..450 score: 45.0 coord: 361..379 score: 36.95 coord: 402..426 score: 40.36 coord: 298..315 score: 31.47 coord: 180..198 score: 27.4 coord: 57..76 score: 31.38 coord: 318..344 score: 31.94 coord: 81..102 score: 24.02
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 17..474 e-value: 5.3E-115 score: 386.7
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 33..478
None	No IPR available	PANTHER	PTHR47947	CYTOCHROME P450 82C3-RELATED	coord: 26..448
None	No IPR available	PANTHER	PTHR47947	CYTOCHROME P450 82C3-RELATED	coord: 449..485
None	No IPR available	PANTHER	PTHR47947:SF26	CYTOCHROME P450	coord: 26..448
None	No IPR available	PANTHER	PTHR47947:SF26	CYTOCHROME P450	coord: 449..485

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc11g02310.1	Moc11g02310.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0098542	defense response to other organism
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen