Moc10g28260 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc10g28260
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionBOI-related E3 ubiquitin-protein ligase 1-like
Locationchr10: 21134439 .. 21140645 (-)
RNA-Seq ExpressionMoc10g28260
SyntenyMoc10g28260
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATCCTGATGAGAGCCATCGGTGCTTCGAAGAGAAGAGCCTTAAAGAAGCGGCTGATCACTTAGATGAGGCAAGACCGAGCCCTGATCGTCAACTTAATCCGACCACCCTTGACGGGGGAGAACCAGTGTCGTGCCTGAATAAAGCGACAGACCTCTCGGGAAAGGCGAGGCCGAGCCCTGGTTATCAACTTAATCCGACCACCCTTGATAAGGGAGAACCAGTGGCGGGCCTGATTAAAGCGACAGATCTTTCGGGAGAGGCAAGGTCGAGCCCTGGTCATCGACTTAATTCGACCACCCTTGACAGGGGAGAACCGGCTGAAGAGGTAGAATCTGTCCCTCTGACAGCCGAAGATCGACGGGTCAACATCGGAACCAAGTTGGGGAGTGAGGAAAGAGAAAGCCTTATAAACTTCCTCAGAAGCAATGCTGCTGTGTTCGCATGGTCACCGGAGGACATGTCGGGAATCGACCGAGGAGTTATGGTACATCGACTCAACGTCGACGAGAGTTGTAAGCCAGTGAAACAGAAGCGTCGCGCGTTCAACCAAGAAAGGAACGAGATTGTAGCTGAACAGGTGGATGAGCTACTGAAAACAGACTTCATTCGGGAAGTACATTATCCCCAGTGGATATCCAACGTGGTGCTGGTAAAGAAGCCAAACGGCAAATGAAGAATGTGCATTGACTTCACGGATCTAAATAAGGTTTGTCCAAAGGATTCTTTTCCTTTGCCTCGAATAGATCAACTGGTTAATGCCACTGCAGGACATGAAAGATTGAGTTTCATGGATGCCTACTCTAGATATAATCAAATCAAGATCCATGTACCGGATCAGGAGAAAACTTCTTTTGTCACGAGTCGAGGCTTGTATTGCTACACCGTGATGCCGTTTGGACTAAAGAATGCAGGTGCAACCTACCAACGAATGGTGAATCGGATGTTTGCAGCCTTAATCGGCAAAAATATGGAAGTCTACGTAGACGACATGCTGGTAAAGAGTCGAAAGAACGAGCGACACGAGGAGGATTTGGCAGAAGCTTTCGACATCTTGCAAAAGTACAAGATGAAGTTGAATCCCACCAAATGCACGTTTGGAGTGACCTCAGGCAAGTTCCTGGGATTTTTAGTGCACCAAAGGGGAATCGAAGCCAATCCAGATAAGATCAAAGCTGTTCTGGAGATGGATCCACTTAAAACTCTAAAGCAGCTGCAGGGACTCAAGGGAAGGATAGCAGCACTCAACAGATTCGTTAGCCGTTCCACTGACAAGTGCCTACCATTCTTCAAAATCTTAAGGAAGAAAGGACAATTTGAATGGACGGAAGAGTGCGAGGCTGCCTTTAAGGAGCTGAAACAGTACCTAGGGGCAGCTCCACTCCTCGCAAAGCCGATCGTGGGTGATGAGCTCTACTTATACTTAGTTGTATCCAGCACTGCGGTGAGTTCCGCGTTAATAAAGGAAGAAGATGGACGACAATCCCCAATTTACTACACAAGCAAAGCAATGGTAGATGCAGAACTTAGATACCCACCAATGGAGAAGCTGGCATTGGCACTGGTCACTTCAGCTCGGAGACTACGCCCGTATTTCCAAGCTCACAAAAATAATGGTCCTCACTAACTTTCCCTTACGACAGATCTTACAAAAGCCAGACACTTCTGGACGACTGGTAAAGTGGGCTGTAGAGCTCAGCGAGTACGACATTCACTATAAGCCAAGAACTGCCATGAAAGGGCAGGTTGTGGCAGACTTCATAGCGGAATTAACACCAACAGAGCCTCAAGTATATAGTATCGACCCACAAGTGGTTGAGGAAGAACAACTCTGGACACTCTACGTAGATGGGTCCTCAAATTTTCGGGGATGCGGGGCAGGACTCCTACTAATCTCTCCAGATAAAGAAAGATTCGAATATGCGCTGCGATTCAACTTTCATGCATCCAACAACGAAGCCGAGTACGAAGCTCTACTAGCAGGACTAAAAGTGGCCAGGTACGTTGGGGTCAGGAACATACTAATACTTAGTGATTCACAGCTGATTGTGGGTCAGGTGAAAGAGGAGTTCCAGGCTAGAGAATCGCGAATGGCGAAATACCTTTCACAGGTAAGAAACCAGCTCGACCAGTTCTCAAAATATGAGATTCGACAAATTCCACGTGCTCAAAACGTCAACGCCGATGCGTTAGCTCGACTAGCTGCGGCTTATGAAACCAACTTAGGCAGAACTGTACCGGTCGAGATTCTACCCGAGCCAAGCATAGAAGCTCAGGAAGCCATGGATATCGACAATCAAGGGCAATCAGAAGAAAACTGGAAAAGCCCTTTGATCAAATATTTGAAAGATGGGATCCTGCCTACTGAAAAGATAGAGGCCCAGCAGCTACAACGACGAGTGGCCAGATATGTGCTTAAAGACGACAATCTGTACAAAAGAGGCTACTCGTTGCCGCTTTTAAAATGTCTAACGCCCGATGAAGCCGACTACGTCATGAGAGAGATACATGAGGGTGTTTGTGGGAGCCATTCTGGAGCACGATCCCTAAGTCACAAAATAATTCGACAAGGGTACTACTGGCCGACCATGCTGAATGACACGAAGCAATTCACCAAAAAATGCGACAAATGCCAACGGTTTGCAACCGTACCTAGACAACCGCCAGAGCCTCTGACAAGCATAATGAGTCCTTGGCCCTTTGCTCAGTGGGGAATAGACCTTATAGGGCCTCTCCCACTAGGAAGGGGCCAGACCAAATTTGCAGTAGTGGCAGTCGACTATTTTACTAAGTGGGCCGAAGCCAAAGCCCTAGCGACAATTACAGAGAAAAAGGTCACCGACTTCATCTGGAAGAACATAATTTGCCGTTTCGGAATACCCTACGCCATTATCAGTGATAATGGGAAGCAGTTCGACAATGCTGCCTTTCGAGAATTTTGCAGGGAGTTAGGCATTAAGCACATCTATTCCTCGCCAGCACATCCGCAGGCTAATGGGCAGGTTGAAGCAGTCAACAAAATTATCAAAAGAACTCTGAAGACCAGATTAGAAAAGCTCAAAGGGTTGTGGGCAGAGGAACTTCCCAACGCATTATGGGCATACAGAACGACACCTTGTACTTCGACAGGAGAAACGTCATTTTCATTATCATTTGGCTCTGAGGCGGTAGTGCCAGTCGAGATCGGACTCCCTCCCCAAGGTAGAGCAGTTCACTCTACTTGACAACAACGAATCCGTAAAGCTCAACCTCGACCTATTAGAAGAACATCGAGAAACAGCACGACGACTACGAACGGCTGAATACAAAAATCGAATAGCTCGATACTACAACTCGAAGGTCAAGCAACATGACTTTAGAGAAGGTGATCTGGTGTTAAGAAAGGTTATGCCCAATACAAAAAATTCAACTAGCGGTGCGCTCGGGCCAACATGGGAGGGCCCATACAAAGTCACTGAAGTGGTGCGAATAGGCACTTATGCTTTAGAAGACCAGCGGGGGAAGGCCTTACTACACCCGTGGCACGCCCAACACCTCAAAAGATACTACCACTAGTTCGACATATTTCTGGACATTTTTATGTTTCTCCATTATTTGGACCAACTAAGCTTATTGTAACCAAATCTTCGACAGGACGATTTTCCTACTAATAAAGTATTCGACGTTCAACGTGATACTTGAGTTCTTATGTTCATTCTGAAATAAATTTCTCAAGCAGGGGGCATCATATAAATATCTACATATCTACGACCTGAACATCTACATATCTACGACCAATCAAGGAACTCAAAATTAGGACGGACCTAGAAGCAGGCCGATAAACTGAAAGCCAATCAGTGAAACCAGGGAAAAGAATCCCGAGGTGCTGAGAAAGGCATGCGTACGGGCCCAGCAAGGTTAAAACAGAAAACAGGCAAGCCTAACCGATCAACGAAACCCACGGGTAGAAAATCATATGGCAAAAGCCAAAATAGGTTAATTGATATTCCTGGTATAGTAGATGCTATCACACATACAATCATACTCAATTCGATGGAATTGTTTGATCCTTATTGTTGTAAGGGGATCTTCTCTCATTTCGCACGTGAGGGGTTCTTTCTTGGTTTCGTCCAGTCATTAATAACTTTATTATTGGCCAAAAGAGTGACCTAACGCCGGGGCAGAGAACGAGGAAAGCGACCAGAGGGAAGCCCACTGAGCCACGAAGGGCTCGGCCCCGACCCGGGGCTCGGCCCACGGCCCAGCCAGTGATCCCCCGTCTTCGATCGACGATGTCGCCCAGGCCCTGCAACGGTGAATTAGGGTTAGCTACGGAAGATTCTGGAACAACTTCACGTTATAAGTATTGATCCAGCCTTAGAGGTATGACGAAAGATAGTTGACTACATTTACGTTATTCTCTTGCTAACTTAAGCATCGGAGCCCGTGTGACTAGAGTCACACCGGTTTAGCTCGATTTGATTTTGCAGGTACTAGTCTCGGATCAACAAGTTTGGTGGATGCGAGGTAATGAACCGGAGGACCGAAATCCACGCATCAACAGAACCTATACTACTAGTATTATACATATATATGTATATATACATTATGTTGGAAGATATGAGAGAGATTAATTGATTATATATTGGTATTGGAATGGGTGAAAAGAAAAGAAAAGATGATGAGTCCTTAAGAAAAGGCCATTCTCTCCCAACCCTACTTGGCCCCACCCCATCAACACACACATTAAATTTCAAAACTATAACTCATTCGTCACAGATAAAACACAAATACCACACACAGAAGAAGTTCAAAGTACAACCACCACCACCCACGGTGGCGCCGCCGCCGGCAACCACCTCTCCCGGCGGCCAAATATATTAATCCCCTTCACACAACCATATATATATATATATATATATATATATATATATATCTTGTCCTTTTTCTCTCTCTCTCATTGAAGAATCCGCCCTTGAATTTGAGGCATCTTCCCCCCTCTCCTCTTTCATGCTTCTTCTGTGGTAGCCTTTATTGTTCTTGCCTCCCCCTCTCTCTCCTCCACTTTCTCTCTCTAAAAATCCCCCCTTTTATATATATTCCATATATACAAACACAACCCATCTCTCTTTTTGCTCTCAATTCCAATGGCAGTTCAAGCTCAATACCCTTCCAATGTCCTCCTCCTCAACAGGTTCCCCAAATTTTCCTCTGTTTCTCAATCCCAGATTTTGATTCTGGGCTTTCGGTTTTTGATCTGTTTTCATAATTTGCAGAAACATTCAAGAAGATCATGATTTTTCATTACAGCCTCAACCTGGTGGAGTAGTAGCAGCTGGAGGAGTTCTTCCACACCCCCATCTTCTCTTCAACAATGGAGGTCGGTCTTCAAATTCAGTTTCAGTTTCAGATCTTTTGTTTGAATTGGTTGATTTTGGATGATGGGTTTTGATTTTTAATCAGGTGGGAATGATAATAATATGAATTTGAGGAAAAGAGGAAGAGATGTTTCAGTTTCAGCTCCCACCATTATCAATCATCCTCTCTGTTTTCATCACCAATCTCAAATTATTGAGATTTCTCAGCTCCATAGCCACCACCAATCCTCCAATGTCGTCTCCACCGGCCTCCGGCTGTCCTCCGGCGACCAACAACATAAGCTCCACCAGCACCCGCCGGCGATGAATGAGAATAATCTTGTTTCTCTCTCTTCCTCTGTTTTATTGTCTAGCGATTTCGCATCCCATATCAAGCAACAGAGGGATGAGATTGACCAGATTCTTCAAGCCCAGGTACGGATTTGAAATACCCAGATGGGAAATTGAATTTTTAAGGGAAAAAAGGAGAAATTGATTTTGAATTTTGGGTTTTGGGGTTTGATTAGGAGGAGGAGCTGCGGCGGGCGTTGGCGGAGAAGAGGCAGAGGCACTTCCGGGGAGCTGGTGGGAGCGGCGGAGGAGACGGCGGCGAGGAAATTGCGGGAAAAAGAAACGGAAGTGGAGAAGGCCACTCGCCGGAACGCGGAGTTGGAGGCACGCGCCGCCCGGGTGAGCATGGAGGCGCAGGCGTGGCAGGCGAAGGCGAGGGCGCAGGAGGCGGCAGCGGCGGCGCTGCAGGCCCAACTACAGCAGGCGGTAATGCGCGGCGCCGGAATGGGCGGCGGAGGAGAGGGCGGCGGAGACTGCACGGTGGGGGCGGCGGAGGACGCCGAGTCAGCGTACGTTGA

mRNA sequence

ATGAATCCTGATGAGAGCCATCGGTGCTTCGAAGAGAAGAGCCTTAAAGAAGCGGCTGATCACTTAGATGAGGCAAGACCGAGCCCTGATCGTCAACTTAATCCGACCACCCTTGACGGGGGAGAACCAGTGTCGTGCCTGAATAAAGCGACAGACCTCTCGGGAAAGGCGAGGCCGAGCCCTGGTTATCAACTTAATCCGACCACCCTTGATAAGGGAGAACCAGTGGCGGGCCTGATTAAAGCGACAGATCTTTCGGGAGAGGCAAGGTCGAGCCCTGGTCATCGACTTAATTCGACCACCCTTGACAGGGGAGAACCGGCTGAAGAGGAAGCCATGGATATCGACAATCAAGGGCAATCAGAAGAAAACTGGAAAAGCCCTTTGATCAAATATTTGAAAGATGGGATCCTGCCTACTGAAAAGATAGAGGCCCAGCAGCTACAACGACGAGTGGCCAGATATGTGCTTAAAGACGACAATCTAAACATTCAAGAAGATCATGATTTTTCATTACAGCCTCAACCTGGTGGAGTAGTAGCAGCTGGAGGAGTTCTTCCACACCCCCATCTTCTCTTCAACAATGGAGGTGGGAATGATAATAATATGAATTTGAGGAAAAGAGGAAGAGATGTTTCAGTTTCAGCTCCCACCATTATCAATCATCCTCTCTGTTTTCATCACCAATCTCAAATTATTGAGATTTCTCAGCTCCATAGCCACCACCAATCCTCCAATGTCGTCTCCACCGGCCTCCGGCTGTCCTCCGGCGACCAACAACATAAGCTCCACCAGCACCCGCCGGCGATGAATGAGAATAATCTTGTTTCTCTCTCTTCCTCTGTTTTATTGTCTAGCGATTTCGCATCCCATATCAAGCAACAGAGGGATGAGATTGACCAGATTCTTCAAGCCCAGGAGGAGGAGCTGCGGCGGGCGTTGGCGGAGAAGAGGCAGAGGCACTTCCGGGGAGCTGGTGGGAGCGGCGGAGGAGACGGCGGCGAGGAAATTGCGGGAAAAAGAAACGGAAGTGGAGAAGGCCACTCGCCGGAACGCGGAGTTGGAGGCACGCGCCGCCCGGGTGAGCATGGAGGCGCAGGCGTGGCAGGCGAAGGCGAGGGCGCAGGAGGCGGCAGCGGCGGCGCTGCAGGCCCAACTACAGCAGGCGGTAATGCGCGGCGCCGGAATGGGCGGCGGAGGAGAGGGCGGCGGAGACTGCACGGTGGGGGCGGCGGAGGACGCCGAGTCAGCGTACGTTGA

Coding sequence (CDS)

ATGAATCCTGATGAGAGCCATCGGTGCTTCGAAGAGAAGAGCCTTAAAGAAGCGGCTGATCACTTAGATGAGGCAAGACCGAGCCCTGATCGTCAACTTAATCCGACCACCCTTGACGGGGGAGAACCAGTGTCGTGCCTGAATAAAGCGACAGACCTCTCGGGAAAGGCGAGGCCGAGCCCTGGTTATCAACTTAATCCGACCACCCTTGATAAGGGAGAACCAGTGGCGGGCCTGATTAAAGCGACAGATCTTTCGGGAGAGGCAAGGTCGAGCCCTGGTCATCGACTTAATTCGACCACCCTTGACAGGGGAGAACCGGCTGAAGAGGAAGCCATGGATATCGACAATCAAGGGCAATCAGAAGAAAACTGGAAAAGCCCTTTGATCAAATATTTGAAAGATGGGATCCTGCCTACTGAAAAGATAGAGGCCCAGCAGCTACAACGACGAGTGGCCAGATATGTGCTTAAAGACGACAATCTAAACATTCAAGAAGATCATGATTTTTCATTACAGCCTCAACCTGGTGGAGTAGTAGCAGCTGGAGGAGTTCTTCCACACCCCCATCTTCTCTTCAACAATGGAGGTGGGAATGATAATAATATGAATTTGAGGAAAAGAGGAAGAGATGTTTCAGTTTCAGCTCCCACCATTATCAATCATCCTCTCTGTTTTCATCACCAATCTCAAATTATTGAGATTTCTCAGCTCCATAGCCACCACCAATCCTCCAATGTCGTCTCCACCGGCCTCCGGCTGTCCTCCGGCGACCAACAACATAAGCTCCACCAGCACCCGCCGGCGATGAATGAGAATAATCTTGTTTCTCTCTCTTCCTCTGTTTTATTGTCTAGCGATTTCGCATCCCATATCAAGCAACAGAGGGATGAGATTGACCAGATTCTTCAAGCCCAGGAGGAGGAGCTGCGGCGGGCGTTGGCGGAGAAGAGGCAGAGGCACTTCCGGGGAGCTGGTGGGAGCGGCGGAGGAGACGGCGGCGAGGAAATTGCGGGAAAAAGAAACGGAAGTGGAGAAGGCCACTCGCCGGAACGCGGAGTTGGAGGCACGCGCCGCCCGGGTGAGCATGGAGGCGCAGGCGTGGCAGGCGAAGGCGAGGGCGCAGGAGGCGGCAGCGGCGGCGCTGCAGGCCCAACTACAGCAGGCGGTAATGCGCGGCGCCGGAATGGGCGGCGGAGGAGAGGGCGGCGGAGACTGCACGGTGGGGGCGGCGGAGGACGCCGAGTCAGCGTACGTTGA

Protein sequence

MNPDESHRCFEEKSLKEAADHLDEARPSPDRQLNPTTLDGGEPVSCLNKATDLSGKARPSPGYQLNPTTLDKGEPVAGLIKATDLSGEARSSPGHRLNSTTLDRGEPAEEEAMDIDNQGQSEENWKSPLIKYLKDGILPTEKIEAQQLQRRVARYVLKDDNLNIQEDHDFSLQPQPGGVVAAGGVLPHPHLLFNNGGGNDNNMNLRKRGRDVSVSAPTIINHPLCFHHQSQIIEISQLHSHHQSSNVVSTGLRLSSGDQQHKLHQHPPAMNENNLVSLSSSVLLSSDFASHIKQQRDEIDQILQAQEEELRRALAEKRQRHFRGAGGSGGGDGGEEIAGKRNGSGEGHSPERGVGGTRRPGEHGGAGVAGEGEGAGGGSGGAAGPTTAGGNARRRNGRRRRGRRRLHGGGGGGRRVSVR
Homology
BLAST of Moc10g28260 vs. NCBI nr
Match: XP_022155494.1 (BOI-related E3 ubiquitin-protein ligase 1-like [Momordica charantia])

HSP 1 Score: 297.7 bits (761), Expect = 1.5e-76
Identity = 180/251 (71.71%), Postives = 188/251 (74.90%), Query Frame = 0

Query: 161 NLNIQEDHDFSLQPQPGGVVAAGGVLPHPHLLFNNGGGNDNNMNLRKRGRDVSVSAPTII 220
           N NIQEDHDFSLQPQPGGVVAAGGVLPHPHLLFNNGGGNDNNMNLRKRGRDVSVSAPTII
Sbjct: 15  NRNIQEDHDFSLQPQPGGVVAAGGVLPHPHLLFNNGGGNDNNMNLRKRGRDVSVSAPTII 74

Query: 221 NHPLCFHHQSQIIEISQLHSHHQSSNVVSTGLRLSSGDQQHKLHQHPPAMNENNLVSLSS 280
           NHPLCFHHQSQIIEISQLHSHHQSSNVVSTGLRLSSGDQQHKLHQHPPAMNENNLVSLSS
Sbjct: 75  NHPLCFHHQSQIIEISQLHSHHQSSNVVSTGLRLSSGDQQHKLHQHPPAMNENNLVSLSS 134

Query: 281 SVLLSSDFASHIKQQRDEIDQILQAQEEELRRALAEKRQRHFRGAGGSGGGDGGEEI--- 340
           SVLLSSDFASHIKQQRDEIDQILQAQEEELRRALAEKRQRHFR   G+       ++   
Sbjct: 135 SVLLSSDFASHIKQQRDEIDQILQAQEEELRRALAEKRQRHFRELVGAAEETAARKLREK 194

Query: 341 ------AGKRNGSGEGH----SPERGVGGTRRPGEHG---------------GAGVAGEG 384
                 A +RN   E      S E      +   +                 GAG+ G G
Sbjct: 195 ETEVEKATRRNAELEARAARVSMEAQAWQAKARAQEAAAAALQAQLQQAVMRGAGMGGGG 254

BLAST of Moc10g28260 vs. NCBI nr
Match: XP_022156571.1 (uncharacterized protein LOC111023448 [Momordica charantia])

HSP 1 Score: 210.7 bits (535), Expect = 2.5e-50
Identity = 104/110 (94.55%), Postives = 107/110 (97.27%), Query Frame = 0

Query: 1   MNPDESHRCFEEKSLKEAADHLDEARPSPDRQLNPTTLDGGEPVSCLNKATDLSGKARPS 60
           MNPDESHRCFEEKSLK+AADHLDEARPSPDRQLNPTTLDGGEPVS LNKATDLSGKARPS
Sbjct: 1   MNPDESHRCFEEKSLKKAADHLDEARPSPDRQLNPTTLDGGEPVSGLNKATDLSGKARPS 60

Query: 61  PGYQLNPTTLDKGEPVAGLIKATDLSGEARSSPGHRLNSTTLDRGEPAEE 111
           PGY+LNPTTLDKG PVAGLIKATDLSG+AR SPGHRLNSTTLDRGEPAEE
Sbjct: 61  PGYRLNPTTLDKGGPVAGLIKATDLSGDARPSPGHRLNSTTLDRGEPAEE 110

BLAST of Moc10g28260 vs. NCBI nr
Match: XP_022156571.1 (uncharacterized protein LOC111023448 [Momordica charantia])

HSP 1 Score: 99.4 bits (246), Expect = 8.0e-17
Identity = 49/53 (92.45%), Postives = 50/53 (94.34%), Query Frame = 0

Query: 110 EEAMDIDNQGQSEENWKSPLIKYLKDGILPTEKIEAQQLQRRVARYVLKDDNL 163
           +EAMDIDNQGQ EEN KSPLIKYLKDGILPTEKIEAQQLQRR ARYVLKDDNL
Sbjct: 799 QEAMDIDNQGQXEENXKSPLIKYLKDGILPTEKIEAQQLQRRAARYVLKDDNL 851


HSP 2 Score: 200.7 bits (509), Expect = 2.5e-47
Identity = 116/169 (68.64%), Postives = 129/169 (76.33%), Query Frame = 0

Query: 161 NLNIQED-HDFSLQPQP-----GGVVAAGGVLPHPHLLFNNGGGNDNNMNLRKRGRDVSV 220
           N NIQ+  HD+SLQPQP     GG    GG L H H LFNNG GN  + NLRKRGRDVSV
Sbjct: 15  NRNIQDSGHDYSLQPQPGGGGGGGGGGGGGDLEHSHALFNNGDGNYLS-NLRKRGRDVSV 74

Query: 221 SAPTIINHPLCFHHQSQIIEISQLHSHHQSSNVVSTGLRLSSGDQQHKLHQHPPAMNENN 280
           SAP I  +PLCF  Q QII++SQLH+HH  SNVVSTGLRLSSGDQ    H HPP  N++N
Sbjct: 75  SAPII--NPLCF--QPQIIDLSQLHNHHHPSNVVSTGLRLSSGDQGLNPHHHPPPRNDDN 134

Query: 281 LVSLSSSVLLSSDFASHIKQQRDEIDQILQAQEEELRRALAEKRQRHFR 324
           LVSLSSSV +S+DF+S IK QRDEIDQ LQAQEEELRRALAEKR RH+R
Sbjct: 135 LVSLSSSVFISNDFSSQIKGQRDEIDQFLQAQEEELRRALAEKRHRHYR 178

BLAST of Moc10g28260 vs. NCBI nr
Match: XP_022923525.1 (BOI-related E3 ubiquitin-protein ligase 1-like isoform X1 [Cucurbita moschata] >KAG6577646.1 E3 ubiquitin-protein ligase BOI, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015684.1 E3 ubiquitin-protein ligase BOI, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 199.9 bits (507), Expect = 4.3e-47
Identity = 116/170 (68.24%), Postives = 128/170 (75.29%), Query Frame = 0

Query: 161 NLNIQED-HDFSLQPQPGGVVAA------GGVLPHPHLLFNNGGGNDNNMNLRKRGRDVS 220
           N NIQ+  HD+SLQPQPGG          GG L H H LFNNGGGN  + NLRKRGRDVS
Sbjct: 15  NRNIQDSGHDYSLQPQPGGGGGGGGGGGRGGDLEHSHALFNNGGGNYLS-NLRKRGRDVS 74

Query: 221 VSAPTIINHPLCFHHQSQIIEISQLHSHHQSSNVVSTGLRLSSGDQQHKLHQHPPAMNEN 280
           VSAP I  +PLCF  Q QII++SQLH+HH  SNVVSTGLRLSSGDQ    H H P  N++
Sbjct: 75  VSAPII--NPLCF--QPQIIDLSQLHNHHHPSNVVSTGLRLSSGDQGLNPHHHTPPQNDD 134

Query: 281 NLVSLSSSVLLSSDFASHIKQQRDEIDQILQAQEEELRRALAEKRQRHFR 324
           NLVSLSSSV +S DF+S IK QRDEIDQ LQAQEEELRRALAEKR RH+R
Sbjct: 135 NLVSLSSSVFISDDFSSQIKGQRDEIDQFLQAQEEELRRALAEKRHRHYR 179

BLAST of Moc10g28260 vs. NCBI nr
Match: XP_038903558.1 (uncharacterized protein LOC120090118 [Benincasa hispida])

HSP 1 Score: 199.1 bits (505), Expect = 7.4e-47
Identity = 114/170 (67.06%), Postives = 130/170 (76.47%), Query Frame = 0

Query: 161 NLNIQE-DHDFSLQPQPGGV--VAAGGVLPHPHLLFNNGGGNDNNMNLRKRGRDVSVSAP 220
           N NIQ+ +HD+SLQPQPGGV     GGVL   H LFNNGGGN ++ NLRKRGRD SVS  
Sbjct: 15  NRNIQDTEHDYSLQPQPGGVGGGGGGGVLQQSHALFNNGGGN-HSSNLRKRGRDNSVSVS 74

Query: 221 TIINHPLCFHHQSQIIEISQLHSHHQSSNVVSTGLRLSSGDQQHKLHQH----PPAMNEN 280
             I +PLCF  Q QII++SQLH+HH  SNVVSTGLRLSSGDQ   L+ H    PP+ N+ 
Sbjct: 75  PPIINPLCF--QPQIIDLSQLHNHHHPSNVVSTGLRLSSGDQPLNLYHHPPPPPPSQNDA 134

Query: 281 NLVSLSSSVLLSSDFASHIKQQRDEIDQILQAQEEELRRALAEKRQRHFR 324
           +LVSLSSSV +S DF+S IKQ RDEIDQ LQ QEEELRRALAEKRQRH+R
Sbjct: 135 SLVSLSSSVFISDDFSSQIKQHRDEIDQFLQTQEEELRRALAEKRQRHYR 181

BLAST of Moc10g28260 vs. ExPASy TrEMBL
Match: A0A6J1DQF4 (BOI-related E3 ubiquitin-protein ligase 1-like OS=Momordica charantia OX=3673 GN=LOC111022625 PE=4 SV=1)

HSP 1 Score: 297.7 bits (761), Expect = 7.4e-77
Identity = 180/251 (71.71%), Postives = 188/251 (74.90%), Query Frame = 0

Query: 161 NLNIQEDHDFSLQPQPGGVVAAGGVLPHPHLLFNNGGGNDNNMNLRKRGRDVSVSAPTII 220
           N NIQEDHDFSLQPQPGGVVAAGGVLPHPHLLFNNGGGNDNNMNLRKRGRDVSVSAPTII
Sbjct: 15  NRNIQEDHDFSLQPQPGGVVAAGGVLPHPHLLFNNGGGNDNNMNLRKRGRDVSVSAPTII 74

Query: 221 NHPLCFHHQSQIIEISQLHSHHQSSNVVSTGLRLSSGDQQHKLHQHPPAMNENNLVSLSS 280
           NHPLCFHHQSQIIEISQLHSHHQSSNVVSTGLRLSSGDQQHKLHQHPPAMNENNLVSLSS
Sbjct: 75  NHPLCFHHQSQIIEISQLHSHHQSSNVVSTGLRLSSGDQQHKLHQHPPAMNENNLVSLSS 134

Query: 281 SVLLSSDFASHIKQQRDEIDQILQAQEEELRRALAEKRQRHFRGAGGSGGGDGGEEI--- 340
           SVLLSSDFASHIKQQRDEIDQILQAQEEELRRALAEKRQRHFR   G+       ++   
Sbjct: 135 SVLLSSDFASHIKQQRDEIDQILQAQEEELRRALAEKRQRHFRELVGAAEETAARKLREK 194

Query: 341 ------AGKRNGSGEGH----SPERGVGGTRRPGEHG---------------GAGVAGEG 384
                 A +RN   E      S E      +   +                 GAG+ G G
Sbjct: 195 ETEVEKATRRNAELEARAARVSMEAQAWQAKARAQEAAAAALQAQLQQAVMRGAGMGGGG 254

BLAST of Moc10g28260 vs. ExPASy TrEMBL
Match: A0A6J1DVC6 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111023448 PE=4 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 1.2e-50
Identity = 104/110 (94.55%), Postives = 107/110 (97.27%), Query Frame = 0

Query: 1   MNPDESHRCFEEKSLKEAADHLDEARPSPDRQLNPTTLDGGEPVSCLNKATDLSGKARPS 60
           MNPDESHRCFEEKSLK+AADHLDEARPSPDRQLNPTTLDGGEPVS LNKATDLSGKARPS
Sbjct: 1   MNPDESHRCFEEKSLKKAADHLDEARPSPDRQLNPTTLDGGEPVSGLNKATDLSGKARPS 60

Query: 61  PGYQLNPTTLDKGEPVAGLIKATDLSGEARSSPGHRLNSTTLDRGEPAEE 111
           PGY+LNPTTLDKG PVAGLIKATDLSG+AR SPGHRLNSTTLDRGEPAEE
Sbjct: 61  PGYRLNPTTLDKGGPVAGLIKATDLSGDARPSPGHRLNSTTLDRGEPAEE 110

BLAST of Moc10g28260 vs. ExPASy TrEMBL
Match: A0A6J1DVC6 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111023448 PE=4 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 3.9e-17
Identity = 49/53 (92.45%), Postives = 50/53 (94.34%), Query Frame = 0

Query: 110 EEAMDIDNQGQSEENWKSPLIKYLKDGILPTEKIEAQQLQRRVARYVLKDDNL 163
           +EAMDIDNQGQ EEN KSPLIKYLKDGILPTEKIEAQQLQRR ARYVLKDDNL
Sbjct: 799 QEAMDIDNQGQXEENXKSPLIKYLKDGILPTEKIEAQQLQRRAARYVLKDDNL 851


HSP 2 Score: 199.9 bits (507), Expect = 2.1e-47
Identity = 116/170 (68.24%), Postives = 128/170 (75.29%), Query Frame = 0

Query: 161 NLNIQED-HDFSLQPQPGGVVAA------GGVLPHPHLLFNNGGGNDNNMNLRKRGRDVS 220
           N NIQ+  HD+SLQPQPGG          GG L H H LFNNGGGN  + NLRKRGRDVS
Sbjct: 15  NRNIQDSGHDYSLQPQPGGGGGGGGGGGRGGDLEHSHALFNNGGGNYLS-NLRKRGRDVS 74

Query: 221 VSAPTIINHPLCFHHQSQIIEISQLHSHHQSSNVVSTGLRLSSGDQQHKLHQHPPAMNEN 280
           VSAP I  +PLCF  Q QII++SQLH+HH  SNVVSTGLRLSSGDQ    H H P  N++
Sbjct: 75  VSAPII--NPLCF--QPQIIDLSQLHNHHHPSNVVSTGLRLSSGDQGLNPHHHTPPQNDD 134

Query: 281 NLVSLSSSVLLSSDFASHIKQQRDEIDQILQAQEEELRRALAEKRQRHFR 324
           NLVSLSSSV +S DF+S IK QRDEIDQ LQAQEEELRRALAEKR RH+R
Sbjct: 135 NLVSLSSSVFISDDFSSQIKGQRDEIDQFLQAQEEELRRALAEKRHRHYR 179

BLAST of Moc10g28260 vs. ExPASy TrEMBL
Match: A0A6J1HKS9 (BOI-related E3 ubiquitin-protein ligase 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111465067 PE=4 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 3.6e-47
Identity = 114/165 (69.09%), Postives = 128/165 (77.58%), Query Frame = 0

Query: 161 NLNIQED-HDFSLQPQP-GGVVAAGGVLPHPHLLFNNGGGNDNNMNLRKRGRDVSVSAPT 220
           N NIQ+  HD+SLQPQP GG    GG L   H LFNNGGGN  + NLRKRGRDVSVSAP 
Sbjct: 15  NRNIQDSGHDYSLQPQPGGGGGVGGGDLEQSHALFNNGGGNYLS-NLRKRGRDVSVSAPI 74

Query: 221 IINHPLCFHHQSQIIEISQLHSHHQSSNVVSTGLRLSSGDQQHKLHQHPPAMNENNLVSL 280
           I  +PLCF  Q QII++SQLH+HH  SNVVSTGLRLSSGDQ    H HPP  N++NLVSL
Sbjct: 75  I--NPLCF--QPQIIDLSQLHNHHHPSNVVSTGLRLSSGDQGLNPHHHPPPQNDDNLVSL 134

Query: 281 SSSVLLSSDFASHIKQQRDEIDQILQAQEEELRRALAEKRQRHFR 324
           SSSV +S +F+S IK QRDEIDQ LQ+QEEELRRALAEKR RH+R
Sbjct: 135 SSSVFISDEFSSQIKVQRDEIDQFLQSQEEELRRALAEKRHRHYR 174

BLAST of Moc10g28260 vs. ExPASy TrEMBL
Match: A0A6J1KT70 (E3 ubiquitin-protein ligase BOI-like OS=Cucurbita maxima OX=3661 GN=LOC111498441 PE=4 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 1.0e-46
Identity = 116/170 (68.24%), Postives = 130/170 (76.47%), Query Frame = 0

Query: 161 NLNIQE-DHDFSLQPQPGGVVAAGG-VLPHPHLLFNNGGGNDNNMNLRKRGRDVSVS--- 220
           N NIQ+ +HD+SLQPQPGG    GG V    H LFNNGGGN  N NLRKRGRDVSVS   
Sbjct: 15  NRNIQDSEHDYSLQPQPGGGGGGGGDVFQQSHHLFNNGGGNHFN-NLRKRGRDVSVSLSV 74

Query: 221 APTIINHPLCFHHQSQIIEISQLHSHHQSSNVVSTGLRLSSGDQQHKLHQH--PPAMNEN 280
           +P +IN PLCFH   QII++SQLH+H+  SNVVSTGLRLSS DQQ  LH H  PPA N+ 
Sbjct: 75  SPPLIN-PLCFH--PQIIDLSQLHNHYHPSNVVSTGLRLSSDDQQPNLHYHPPPPARNDT 134

Query: 281 NLVSLSSSVLLSSDFASHIKQQRDEIDQILQAQEEELRRALAEKRQRHFR 324
           NLVS SSSV LS DF+S  +QQRDEID+ LQ QEEELRRALAEKRQRH+R
Sbjct: 135 NLVSFSSSVFLSDDFSSQTRQQRDEIDRFLQTQEEELRRALAEKRQRHYR 180

BLAST of Moc10g28260 vs. TAIR 10
Match: AT1G32740.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 83.6 bits (205), Expect = 4.2e-16
Identity = 66/169 (39.05%), Postives = 91/169 (53.85%), Query Frame = 0

Query: 165 QEDHDFSLQPQPGGVVAAGGVLPHPHLLFNNGGGNDNNMNLRKRGRDVSVSAPTIINH-- 224
           +E  +F+LQ Q     AAG  L   ++LFNNG  N      RKR R+ +       NH  
Sbjct: 23  KEHSNFTLQSQ-----AAGDFLDQTNMLFNNGSSNQ-----RKRRRETN-------NHQL 82

Query: 225 -PLCFHHQSQIIEISQLHSH-HQSSNVVSTGLRLSSGDQQHKLHQHPPAMNENNLVSLSS 284
            P+  H   Q+I++S LH++ H  SN+V TGLRL SG+ Q +   H              
Sbjct: 83  LPMQSHQFPQVIDLSLLHNYNHPPSNMVHTGLRLFSGEDQAQKISH-------------- 142

Query: 285 SVLLSSD-FASHIKQQRDEIDQILQAQEEELRRALAEKRQRHFRGAGGS 329
              LS D FA+HI +Q +E+D+ L AQ EELRR LAEKR+ H++   G+
Sbjct: 143 ---LSEDVFAAHINRQSEELDEFLHAQAEELRRTLAEKRKMHYKALLGA 157

BLAST of Moc10g28260 vs. TAIR 10
Match: AT4G17680.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 63.2 bits (152), Expect = 5.9e-10
Identity = 59/163 (36.20%), Postives = 80/163 (49.08%), Query Frame = 0

Query: 160 DNLNIQEDHDFSLQPQPGGVVAAGGVLPHPHLLFNNGGGNDNNMNLRKRGRDVSVSAPTI 219
           +N N QE  D SLQPQ             P   F   G     ++ RKR R+V  SA  +
Sbjct: 31  NNSNGQEASDCSLQPQ-----------DTPFTNFTKAG-----VDSRKRSREVYSSA--L 90

Query: 220 INHPLCFHHQSQIIEISQLHSHHQSSNVVSTGLRLSSGDQQHKLHQHPPAMNENNLVSLS 279
           +N P      SQ+I+I++L    ++ NVVSTGLRL           H  + N+    S  
Sbjct: 91  MNPPP--PKPSQVIDITEL--LQKTPNVVSTGLRLF----------HDQSQNQQQFFS-- 150

Query: 280 SSVLLSSDFASHIKQQRDEIDQILQAQEEELRRALAEKRQRHF 323
               L  D    IK+QRDE+D+ +Q Q EELRR LA+ R+R +
Sbjct: 151 ---SLPGDVTGKIKRQRDELDRFIQTQGEELRRTLADNRERRY 156

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022155494.11.5e-7671.71BOI-related E3 ubiquitin-protein ligase 1-like [Momordica charantia][more]
XP_022156571.12.5e-5094.55uncharacterized protein LOC111023448 [Momordica charantia][more]
XP_022156571.18.0e-1792.45uncharacterized protein LOC111023448 [Momordica charantia][more]
XP_022923525.14.3e-4768.24BOI-related E3 ubiquitin-protein ligase 1-like isoform X1 [Cucurbita moschata] >... [more]
XP_038903558.17.4e-4767.06uncharacterized protein LOC120090118 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DQF47.4e-7771.71BOI-related E3 ubiquitin-protein ligase 1-like OS=Momordica charantia OX=3673 GN... [more]
A0A6J1DVC61.2e-5094.55Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111023448 PE=4 SV=1[more]
A0A6J1DVC63.9e-1792.45Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111023448 PE=4 SV=1[more]
A0A6J1HKS93.6e-4769.09BOI-related E3 ubiquitin-protein ligase 1-like isoform X1 OS=Cucurbita maxima OX... [more]
A0A6J1KT701.0e-4668.24E3 ubiquitin-protein ligase BOI-like OS=Cucurbita maxima OX=3661 GN=LOC111498441... [more]
Match NameE-valueIdentityDescription
AT1G32740.14.2e-1639.05SBP (S-ribonuclease binding protein) family protein [more]
AT4G17680.15.9e-1036.20SBP (S-ribonuclease binding protein) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 289..320
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..28
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 321..419
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 393..410
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..123
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 339..358
NoneNo IPR availablePANTHERPTHR42647:SF30BOI-RELATED E3 UBIQUITIN-PROTEIN LIGASE 1-LIKEcoord: 167..325
IPR017066E3 ubiquitin-protein ligase BOI-likePANTHERPTHR42647SBP (S-RIBONUCLEASE BINDING PROTEIN) FAMILY PROTEINcoord: 167..325

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc10g28260.1Moc10g28260.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015074 DNA integration
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
molecular_function GO:0046872 metal ion binding
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity
molecular_function GO:0004842 ubiquitin-protein transferase activity