Moc10g28230 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc10g28230
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionBeta-amylase
Locationchr10: 21090208 .. 21091870 (-)
RNA-Seq ExpressionMoc10g28230
SyntenyMoc10g28230
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTTCCGCTCGATACGGTGAGTGTTGGAGGGAGTCTGAACAAAGCGCGAGCGATGAACGCGAGTTTAATGGCGTTGAAGAGTGCAGGAGTGGAAGGAGTTATGGTGGATGCTTGGTGGGGATTGGTGGAGAAAGATGGCCCTTTGAAATACAACTGGGAAGGCTATGCTGAGCTTGTGCAGATGGTTCAAAAGCATGGCTTGAAGCTGCAAGTAGTTATGTCTTTTCATCAGTGTGGAGGGAATGTTGGAGATTCTTGCAGGTAAGAAAATTCAATCAAACTTTTGCAATTTTCATCATTGTTTTCATGAAACTCAGTTAGTATTCTTCTCTGTTTTGATGTTTCAGCATTCCTTTACCTCCATGGGTGCTTGAAGAAATAAGCAAAAACCCGGATCTCGTTTACACTGACAGATCAGGCAGGAGGAATCCTGAATACATCTCATTGGGGTGTGATTCATTACCTGTTCTCAAAGGAAGAACACCGATCCAAGTTTACGCCGATTACATGAGGAGCTTTCGAGACCGATTCAGAGACTACTTGGGAGAAGTTATAGTGGTATGTATCTACAATTCATTTAGTTCTCTTAATCCTTCGTCGTCTTAACCGTCCCCGACAATTCGATCATTGAAGTTAGAGTGACATAGATGGGATGGATTTGATGTGTGGGATTGCAGGAAATTCAAGTTGGGGCTGGTCCATGTGGGGAACTCAGATATCCATCTTATCCAGAGAGCAATGGGACTTGGAGATTTCCTGGAATTGGAGAATTCCAGTGTTATGACAAGGTACTCAAATTCTAGACTAGATAGACTTCTTTTTTCTTGTTTCAATGTCGAATTTATCGGCTAAAAATCCAGTTATGATCCTGATAACAGTACATGAGAGCTTCCCTTGAAGCAGCAGCAGAGGCTGCAGGAAAAAGGGAGTGGGGAAGCAATGGACCACATGATTCAGGTCAGTACAACCAGTTCCCTGAAGATACAGGCTTCTTCAAAAGGGACGGAACATGGAAGTCAGAATACGGAAAGTTCTTCCTTGAATGGTACTCGGGAAAGCTACTGCAGCACGGCGACATGATCCTAGCAGCTGCAAAAGGAATTTTCCAAGGAACTGGTGCAAAACTATCAGCAAAAGTAGCTGGGATTCACTGGCATTACCAGACTCAATCCCATGCTGCGGAGTTAACCGCCGGGTACTACAACACCCGACACCGAGATGGTTACTCGCCTATCGCAAAGATGCTGGCTAAACATGGAGCTGTGTTCAACTTCACTTGTATGGAGATGAAAGATGGACAGCAGCCTAGCCATGGAAATTGCTCACCAGAAGGATTGGTTGCACAAGTGAAGATGGTTACAAAGGATACTAAAACTGAACTTGCTGGTGAGAATGCTTTGGAGAGGTATGATGGAGGAGCATATCACCAAATTTTAGCCACAAGTAGATCAGAATCTGGGAATGGATTGGCTGCATTCACTTACTTGAGGATGAACAAGAACCTTTTTGAACCCCACAATTGGAGAAACTTGGTCGAGTTCGTAAAGAGCATGTCGGAGGGCGGTCGGAGCTCGAGACTTTCGGAATCTGACAGCAGTGGCAGTGATCTTCATGTTCGGTTCATCAAGGAGAAGAAAATCAAGGAAGTTGCCATGGCGTAG

mRNA sequence

ATGCTTCCGCTCGATACGGTGAGTGTTGGAGGGAGTCTGAACAAAGCGCGAGCGATGAACGCGAGTTTAATGGCGTTGAAGAGTGCAGGAGTGGAAGGAGTTATGGTGGATGCTTGGTGGGGATTGGTGGAGAAAGATGGCCCTTTGAAATACAACTGGGAAGGCTATGCTGAGCTTGTGCAGATGGTTCAAAAGCATGGCTTGAAGCTGCAAGTAGTTATGTCTTTTCATCAGTGTGGAGGGAATGTTGGAGATTCTTGCAGCATTCCTTTACCTCCATGGGTGCTTGAAGAAATAAGCAAAAACCCGGATCTCGTTTACACTGACAGATCAGGCAGGAGGAATCCTGAATACATCTCATTGGGGTGTGATTCATTACCTGTTCTCAAAGGAAGAACACCGATCCAAGTTTACGCCGATTACATGAGGAGCTTTCGAGACCGATTCAGAGACTACTTGGGAGAAGTTATAGTGGAAATTCAAGTTGGGGCTGGTCCATGTGGGGAACTCAGATATCCATCTTATCCAGAGAGCAATGGGACTTGGAGATTTCCTGGAATTGGAGAATTCCAGTGTTATGACAAGTACATGAGAGCTTCCCTTGAAGCAGCAGCAGAGGCTGCAGGAAAAAGGGAGTGGGGAAGCAATGGACCACATGATTCAGGTCAGTACAACCAGTTCCCTGAAGATACAGGCTTCTTCAAAAGGGACGGAACATGGAAGTCAGAATACGGAAAGTTCTTCCTTGAATGGTACTCGGGAAAGCTACTGCAGCACGGCGACATGATCCTAGCAGCTGCAAAAGGAATTTTCCAAGGAACTGGTGCAAAACTATCAGCAAAAGTAGCTGGGATTCACTGGCATTACCAGACTCAATCCCATGCTGCGGAGTTAACCGCCGGGTACTACAACACCCGACACCGAGATGGTTACTCGCCTATCGCAAAGATGCTGGCTAAACATGGAGCTGTGTTCAACTTCACTTGTATGGAGATGAAAGATGGACAGCAGCCTAGCCATGGAAATTGCTCACCAGAAGGATTGGTTGCACAAGTGAAGATGGTTACAAAGGATACTAAAACTGAACTTGCTGGTGAGAATGCTTTGGAGAGGTATGATGGAGGAGCATATCACCAAATTTTAGCCACAAGTAGATCAGAATCTGGGAATGGATTGGCTGCATTCACTTACTTGAGGATGAACAAGAACCTTTTTGAACCCCACAATTGGAGAAACTTGGTCGAGTTCGTAAAGAGCATGTCGGAGGGCGGTCGGAGCTCGAGACTTTCGGAATCTGACAGCAGTGGCAGTGATCTTCATGTTCGGTTCATCAAGGAGAAGAAAATCAAGGAAGTTGCCATGGCGTAG

Coding sequence (CDS)

ATGCTTCCGCTCGATACGGTGAGTGTTGGAGGGAGTCTGAACAAAGCGCGAGCGATGAACGCGAGTTTAATGGCGTTGAAGAGTGCAGGAGTGGAAGGAGTTATGGTGGATGCTTGGTGGGGATTGGTGGAGAAAGATGGCCCTTTGAAATACAACTGGGAAGGCTATGCTGAGCTTGTGCAGATGGTTCAAAAGCATGGCTTGAAGCTGCAAGTAGTTATGTCTTTTCATCAGTGTGGAGGGAATGTTGGAGATTCTTGCAGCATTCCTTTACCTCCATGGGTGCTTGAAGAAATAAGCAAAAACCCGGATCTCGTTTACACTGACAGATCAGGCAGGAGGAATCCTGAATACATCTCATTGGGGTGTGATTCATTACCTGTTCTCAAAGGAAGAACACCGATCCAAGTTTACGCCGATTACATGAGGAGCTTTCGAGACCGATTCAGAGACTACTTGGGAGAAGTTATAGTGGAAATTCAAGTTGGGGCTGGTCCATGTGGGGAACTCAGATATCCATCTTATCCAGAGAGCAATGGGACTTGGAGATTTCCTGGAATTGGAGAATTCCAGTGTTATGACAAGTACATGAGAGCTTCCCTTGAAGCAGCAGCAGAGGCTGCAGGAAAAAGGGAGTGGGGAAGCAATGGACCACATGATTCAGGTCAGTACAACCAGTTCCCTGAAGATACAGGCTTCTTCAAAAGGGACGGAACATGGAAGTCAGAATACGGAAAGTTCTTCCTTGAATGGTACTCGGGAAAGCTACTGCAGCACGGCGACATGATCCTAGCAGCTGCAAAAGGAATTTTCCAAGGAACTGGTGCAAAACTATCAGCAAAAGTAGCTGGGATTCACTGGCATTACCAGACTCAATCCCATGCTGCGGAGTTAACCGCCGGGTACTACAACACCCGACACCGAGATGGTTACTCGCCTATCGCAAAGATGCTGGCTAAACATGGAGCTGTGTTCAACTTCACTTGTATGGAGATGAAAGATGGACAGCAGCCTAGCCATGGAAATTGCTCACCAGAAGGATTGGTTGCACAAGTGAAGATGGTTACAAAGGATACTAAAACTGAACTTGCTGGTGAGAATGCTTTGGAGAGGTATGATGGAGGAGCATATCACCAAATTTTAGCCACAAGTAGATCAGAATCTGGGAATGGATTGGCTGCATTCACTTACTTGAGGATGAACAAGAACCTTTTTGAACCCCACAATTGGAGAAACTTGGTCGAGTTCGTAAAGAGCATGTCGGAGGGCGGTCGGAGCTCGAGACTTTCGGAATCTGACAGCAGTGGCAGTGATCTTCATGTTCGGTTCATCAAGGAGAAGAAAATCAAGGAAGTTGCCATGGCGTAG

Protein sequence

MLPLDTVSVGGSLNKARAMNASLMALKSAGVEGVMVDAWWGLVEKDGPLKYNWEGYAELVQMVQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRRNPEYISLGCDSLPVLKGRTPIQVYADYMRSFRDRFRDYLGEVIVEIQVGAGPCGELRYPSYPESNGTWRFPGIGEFQCYDKYMRASLEAAAEAAGKREWGSNGPHDSGQYNQFPEDTGFFKRDGTWKSEYGKFFLEWYSGKLLQHGDMILAAAKGIFQGTGAKLSAKVAGIHWHYQTQSHAAELTAGYYNTRHRDGYSPIAKMLAKHGAVFNFTCMEMKDGQQPSHGNCSPEGLVAQVKMVTKDTKTELAGENALERYDGGAYHQILATSRSESGNGLAAFTYLRMNKNLFEPHNWRNLVEFVKSMSEGGRSSRLSESDSSGSDLHVRFIKEKKIKEVAMA
Homology
BLAST of Moc10g28230 vs. NCBI nr
Match: XP_022155496.1 (beta-amylase 3, chloroplastic [Momordica charantia])

HSP 1 Score: 944.9 bits (2441), Expect = 2.6e-271
Identity = 455/455 (100.00%), Postives = 455/455 (100.00%), Query Frame = 0

Query: 1   MLPLDTVSVGGSLNKARAMNASLMALKSAGVEGVMVDAWWGLVEKDGPLKYNWEGYAELV 60
           MLPLDTVSVGGSLNKARAMNASLMALKSAGVEGVMVDAWWGLVEKDGPLKYNWEGYAELV
Sbjct: 87  MLPLDTVSVGGSLNKARAMNASLMALKSAGVEGVMVDAWWGLVEKDGPLKYNWEGYAELV 146

Query: 61  QMVQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRRNPEYIS 120
           QMVQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRRNPEYIS
Sbjct: 147 QMVQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRRNPEYIS 206

Query: 121 LGCDSLPVLKGRTPIQVYADYMRSFRDRFRDYLGEVIVEIQVGAGPCGELRYPSYPESNG 180
           LGCDSLPVLKGRTPIQVYADYMRSFRDRFRDYLGEVIVEIQVGAGPCGELRYPSYPESNG
Sbjct: 207 LGCDSLPVLKGRTPIQVYADYMRSFRDRFRDYLGEVIVEIQVGAGPCGELRYPSYPESNG 266

Query: 181 TWRFPGIGEFQCYDKYMRASLEAAAEAAGKREWGSNGPHDSGQYNQFPEDTGFFKRDGTW 240
           TWRFPGIGEFQCYDKYMRASLEAAAEAAGKREWGSNGPHDSGQYNQFPEDTGFFKRDGTW
Sbjct: 267 TWRFPGIGEFQCYDKYMRASLEAAAEAAGKREWGSNGPHDSGQYNQFPEDTGFFKRDGTW 326

Query: 241 KSEYGKFFLEWYSGKLLQHGDMILAAAKGIFQGTGAKLSAKVAGIHWHYQTQSHAAELTA 300
           KSEYGKFFLEWYSGKLLQHGDMILAAAKGIFQGTGAKLSAKVAGIHWHYQTQSHAAELTA
Sbjct: 327 KSEYGKFFLEWYSGKLLQHGDMILAAAKGIFQGTGAKLSAKVAGIHWHYQTQSHAAELTA 386

Query: 301 GYYNTRHRDGYSPIAKMLAKHGAVFNFTCMEMKDGQQPSHGNCSPEGLVAQVKMVTKDTK 360
           GYYNTRHRDGYSPIAKMLAKHGAVFNFTCMEMKDGQQPSHGNCSPEGLVAQVKMVTKDTK
Sbjct: 387 GYYNTRHRDGYSPIAKMLAKHGAVFNFTCMEMKDGQQPSHGNCSPEGLVAQVKMVTKDTK 446

Query: 361 TELAGENALERYDGGAYHQILATSRSESGNGLAAFTYLRMNKNLFEPHNWRNLVEFVKSM 420
           TELAGENALERYDGGAYHQILATSRSESGNGLAAFTYLRMNKNLFEPHNWRNLVEFVKSM
Sbjct: 447 TELAGENALERYDGGAYHQILATSRSESGNGLAAFTYLRMNKNLFEPHNWRNLVEFVKSM 506

Query: 421 SEGGRSSRLSESDSSGSDLHVRFIKEKKIKEVAMA 456
           SEGGRSSRLSESDSSGSDLHVRFIKEKKIKEVAMA
Sbjct: 507 SEGGRSSRLSESDSSGSDLHVRFIKEKKIKEVAMA 541

BLAST of Moc10g28230 vs. NCBI nr
Match: XP_008448759.1 (PREDICTED: beta-amylase 3, chloroplastic [Cucumis melo] >KAA0041034.1 beta-amylase 3 [Cucumis melo var. makuwa] >TYK12075.1 beta-amylase 3 [Cucumis melo var. makuwa])

HSP 1 Score: 872.5 bits (2253), Expect = 1.6e-249
Identity = 413/453 (91.17%), Postives = 433/453 (95.58%), Query Frame = 0

Query: 1   MLPLDTVSVGGSLNKARAMNASLMALKSAGVEGVMVDAWWGLVEKDGPLKYNWEGYAELV 60
           MLPLDTV++GGSLNK RAMNASLMALKSAGVEGVMVDAWWGLVEKDGP+KYNWEGYAELV
Sbjct: 83  MLPLDTVTLGGSLNKPRAMNASLMALKSAGVEGVMVDAWWGLVEKDGPMKYNWEGYAELV 142

Query: 61  QMVQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRRNPEYIS 120
           QMVQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRRNPEYIS
Sbjct: 143 QMVQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRRNPEYIS 202

Query: 121 LGCDSLPVLKGRTPIQVYADYMRSFRDRFRDYLGEVIVEIQVGAGPCGELRYPSYPESNG 180
           LGCDSLPVL+GRTPIQVYADYMRSFRDRFRDYLGEVI+E+QVGAGPCGELRYPSYPESNG
Sbjct: 203 LGCDSLPVLRGRTPIQVYADYMRSFRDRFRDYLGEVIMEVQVGAGPCGELRYPSYPESNG 262

Query: 181 TWRFPGIGEFQCYDKYMRASLEAAAEAAGKREWGSNGPHDSGQYNQFPEDTGFFKRDGTW 240
           TWRFPGIGEFQCYDKYMRASLEAAAEA GKR+WGS+GPHDSGQYNQFPEDTGFFK++GTW
Sbjct: 263 TWRFPGIGEFQCYDKYMRASLEAAAEAIGKRDWGSSGPHDSGQYNQFPEDTGFFKKEGTW 322

Query: 241 KSEYGKFFLEWYSGKLLQHGDMILAAAKGIFQGTGAKLSAKVAGIHWHYQTQSHAAELTA 300
           K+EYG+FFL WYS KLLQHGD ILAAAKGIF+GTGAKLSAKVAGIHWHY T+SHAAELTA
Sbjct: 323 KTEYGEFFLAWYSSKLLQHGDSILAAAKGIFRGTGAKLSAKVAGIHWHYGTRSHAAELTA 382

Query: 301 GYYNTRHRDGYSPIAKMLAKHGAVFNFTCMEMKDGQQPSHGNCSPEGLVAQVKMVTKDTK 360
           GYYNTRHRDGYSPIAKMLAKHG VFNFTCMEM+DGQQP + NCSPEGLV QVKM T+D K
Sbjct: 383 GYYNTRHRDGYSPIAKMLAKHGVVFNFTCMEMRDGQQPGYANCSPEGLVRQVKMATRDAK 442

Query: 361 TELAGENALERYDGGAYHQILATSRSESGNGLAAFTYLRMNKNLFEPHNWRNLVEFVKSM 420
            ELAGENALERYDG AY QILATSRS+SGNGLAAFTYLRMNKNLFEP+NWRNLVEFVKSM
Sbjct: 443 VELAGENALERYDGAAYEQILATSRSDSGNGLAAFTYLRMNKNLFEPNNWRNLVEFVKSM 502

Query: 421 SEGGRSSRLSESDSSGSDLHVRFIKEKKIKEVA 454
           SEGGR+ RL ESD  GSDLHV FIKEKKIKEVA
Sbjct: 503 SEGGRNRRLPESDCCGSDLHVGFIKEKKIKEVA 535

BLAST of Moc10g28230 vs. NCBI nr
Match: XP_004147264.1 (beta-amylase 3, chloroplastic [Cucumis sativus] >KGN55808.1 hypothetical protein Csa_009553 [Cucumis sativus])

HSP 1 Score: 869.8 bits (2246), Expect = 1.1e-248
Identity = 414/454 (91.19%), Postives = 432/454 (95.15%), Query Frame = 0

Query: 1   MLPLDTVSVGGSLNKARAMNASLMALKSAGVEGVMVDAWWGLVEKDGPLKYNWEGYAELV 60
           MLPLDTV++GGSLNK RAMNASLMALKSAGVEGVMVDAWWGLVEKDGP+KYNWEGYAELV
Sbjct: 83  MLPLDTVTLGGSLNKPRAMNASLMALKSAGVEGVMVDAWWGLVEKDGPMKYNWEGYAELV 142

Query: 61  QMVQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRRNPEYIS 120
           QMVQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRRNPEYIS
Sbjct: 143 QMVQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRRNPEYIS 202

Query: 121 LGCDSLPVLKGRTPIQVYADYMRSFRDRFRDYLGEVIVEIQVGAGPCGELRYPSYPESNG 180
           LGCDSLPVL+GRTPIQVYADYMRSFRDRFRDYLGEVI E+QVGAGPCGELRYPSYPESNG
Sbjct: 203 LGCDSLPVLRGRTPIQVYADYMRSFRDRFRDYLGEVITEVQVGAGPCGELRYPSYPESNG 262

Query: 181 TWRFPGIGEFQCYDKYMRASLEAAAEAAGKREWGSN-GPHDSGQYNQFPEDTGFFKRDGT 240
           TWRFPGIGEFQCYDKYMRASLEAAAEA GKR+WGS+ GPHDSGQYNQFPEDTGFFK++GT
Sbjct: 263 TWRFPGIGEFQCYDKYMRASLEAAAEAIGKRDWGSSGGPHDSGQYNQFPEDTGFFKKEGT 322

Query: 241 WKSEYGKFFLEWYSGKLLQHGDMILAAAKGIFQGTGAKLSAKVAGIHWHYQTQSHAAELT 300
           WK+EYG+FFL WYS KLLQHGD ILAAAKGIF+GTGAKLSAKVAGIHWHY T+SHAAELT
Sbjct: 323 WKTEYGEFFLAWYSSKLLQHGDSILAAAKGIFRGTGAKLSAKVAGIHWHYGTRSHAAELT 382

Query: 301 AGYYNTRHRDGYSPIAKMLAKHGAVFNFTCMEMKDGQQPSHGNCSPEGLVAQVKMVTKDT 360
           AGYYNTRHRDGYSPIAKMLAKHG VFNFTCMEM+DGQQP H NCSPEGLV QVKM T+D 
Sbjct: 383 AGYYNTRHRDGYSPIAKMLAKHGVVFNFTCMEMRDGQQPGHANCSPEGLVRQVKMATRDA 442

Query: 361 KTELAGENALERYDGGAYHQILATSRSESGNGLAAFTYLRMNKNLFEPHNWRNLVEFVKS 420
           K ELAGENALERYDG AY QILATSRS+SGNGLAAFTYLRMNKNLFEP+NWRNLVEFVKS
Sbjct: 443 KVELAGENALERYDGAAYEQILATSRSDSGNGLAAFTYLRMNKNLFEPNNWRNLVEFVKS 502

Query: 421 MSEGGRSSRLSESDSSGSDLHVRFIKEKKIKEVA 454
           MSEGGR+ RL ESD  GSDLHV FIKEKKIKEVA
Sbjct: 503 MSEGGRNRRLPESDCCGSDLHVGFIKEKKIKEVA 536

BLAST of Moc10g28230 vs. NCBI nr
Match: XP_023007780.1 (beta-amylase 3, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 869.0 bits (2244), Expect = 1.8e-248
Identity = 411/455 (90.33%), Postives = 435/455 (95.60%), Query Frame = 0

Query: 1   MLPLDTVSVGGSLNKARAMNASLMALKSAGVEGVMVDAWWGLVEKDGPLKYNWEGYAELV 60
           MLPLDTV++GG+LNK RAMNASLMALKSAGVEGVMVDAWWGLVEKDGP+KYNWEGYAELV
Sbjct: 82  MLPLDTVTLGGNLNKPRAMNASLMALKSAGVEGVMVDAWWGLVEKDGPMKYNWEGYAELV 141

Query: 61  QMVQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRRNPEYIS 120
           QM+QKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRRNPEYIS
Sbjct: 142 QMLQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRRNPEYIS 201

Query: 121 LGCDSLPVLKGRTPIQVYADYMRSFRDRFRDYLGEVIVEIQVGAGPCGELRYPSYPESNG 180
           LGCDSLPVL+GRTPIQVYADYMRSFRDRFRDYLGEVI+E+QVGAGPCGELRYPSYP+SNG
Sbjct: 202 LGCDSLPVLRGRTPIQVYADYMRSFRDRFRDYLGEVIMEVQVGAGPCGELRYPSYPQSNG 261

Query: 181 TWRFPGIGEFQCYDKYMRASLEAAAEAAGKREWGSN-GPHDSGQYNQFPEDTGFFKRDGT 240
           TWRFPGIGEFQCYDKYMRASLEAAAE+ GKR+WGSN GPHDSGQYNQFPEDTGFFK++GT
Sbjct: 262 TWRFPGIGEFQCYDKYMRASLEAAAESIGKRDWGSNGGPHDSGQYNQFPEDTGFFKKEGT 321

Query: 241 WKSEYGKFFLEWYSGKLLQHGDMILAAAKGIFQGTGAKLSAKVAGIHWHYQTQSHAAELT 300
           W +EYGKFFLEWYSGKLLQHGD ILAA+KGIF GTGAKLSAKVAGIHWHY T+SHAAELT
Sbjct: 322 WNTEYGKFFLEWYSGKLLQHGDRILAASKGIFHGTGAKLSAKVAGIHWHYGTRSHAAELT 381

Query: 301 AGYYNTRHRDGYSPIAKMLAKHGAVFNFTCMEMKDGQQPSHGNCSPEGLVAQVKMVTKDT 360
           AGYYNTRHR+GYSPIAKMLAKHG VFNFTCMEMKDGQQP + NCSPEGLV QVKM T+D 
Sbjct: 382 AGYYNTRHRNGYSPIAKMLAKHGVVFNFTCMEMKDGQQPGYANCSPEGLVRQVKMATRDA 441

Query: 361 KTELAGENALERYDGGAYHQILATSRSESGNGLAAFTYLRMNKNLFEPHNWRNLVEFVKS 420
           K ELAGENALERYDGGAY QILATSRSESGNGL AFTYLRMNKNLFEP+NWRNLVEFVK+
Sbjct: 442 KAELAGENALERYDGGAYEQILATSRSESGNGLTAFTYLRMNKNLFEPNNWRNLVEFVKT 501

Query: 421 MSEGGRSSRLSESDSSGSDLHVRFIKEKKIKEVAM 455
           MSEGGR+ +L ESD SGSDLHV FIKEKKIKEVA+
Sbjct: 502 MSEGGRNRKLPESDCSGSDLHVGFIKEKKIKEVAV 536

BLAST of Moc10g28230 vs. NCBI nr
Match: XP_022923359.1 (beta-amylase 3, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 867.5 bits (2240), Expect = 5.2e-248
Identity = 410/455 (90.11%), Postives = 434/455 (95.38%), Query Frame = 0

Query: 1   MLPLDTVSVGGSLNKARAMNASLMALKSAGVEGVMVDAWWGLVEKDGPLKYNWEGYAELV 60
           MLPLDTV++GG+LNK RAMNASLMALKSAGVEGVMVDAWWGLVEKDGP+KYNWEGYAELV
Sbjct: 82  MLPLDTVTLGGNLNKPRAMNASLMALKSAGVEGVMVDAWWGLVEKDGPMKYNWEGYAELV 141

Query: 61  QMVQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRRNPEYIS 120
           QM+QKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVL+EISKNPDLVYTDRSGRRNPEYIS
Sbjct: 142 QMLQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLKEISKNPDLVYTDRSGRRNPEYIS 201

Query: 121 LGCDSLPVLKGRTPIQVYADYMRSFRDRFRDYLGEVIVEIQVGAGPCGELRYPSYPESNG 180
           LGCDSLPVL+GRTPIQVYADYMRSFRDRFRDYLGEVI+E+QVGAGPCGELRYPSYPESNG
Sbjct: 202 LGCDSLPVLRGRTPIQVYADYMRSFRDRFRDYLGEVIMEVQVGAGPCGELRYPSYPESNG 261

Query: 181 TWRFPGIGEFQCYDKYMRASLEAAAEAAGKREWGSN-GPHDSGQYNQFPEDTGFFKRDGT 240
           TWRFPGIGEFQCYDKYMRASLEAAAE+ GKR+WGSN GPHDSGQYNQFPEDTGFFK++GT
Sbjct: 262 TWRFPGIGEFQCYDKYMRASLEAAAESIGKRDWGSNGGPHDSGQYNQFPEDTGFFKKEGT 321

Query: 241 WKSEYGKFFLEWYSGKLLQHGDMILAAAKGIFQGTGAKLSAKVAGIHWHYQTQSHAAELT 300
           W +EYGKFFLEWYSGKLLQHGD IL A+KGIF GTGAKLSAKVAGIHWHY T+SHAAELT
Sbjct: 322 WNTEYGKFFLEWYSGKLLQHGDRILEASKGIFHGTGAKLSAKVAGIHWHYSTRSHAAELT 381

Query: 301 AGYYNTRHRDGYSPIAKMLAKHGAVFNFTCMEMKDGQQPSHGNCSPEGLVAQVKMVTKDT 360
           AGYYNTRHR+GYSPIAKMLAKHG VFNFTCMEMKDGQQP + NCSPEGLV QVKM T+D 
Sbjct: 382 AGYYNTRHRNGYSPIAKMLAKHGVVFNFTCMEMKDGQQPGYANCSPEGLVRQVKMATRDA 441

Query: 361 KTELAGENALERYDGGAYHQILATSRSESGNGLAAFTYLRMNKNLFEPHNWRNLVEFVKS 420
           K ELAGENALERYDGGAY QILATSRSESGNGL AFTYLRMNKNLFEP+NWRNLVEFVK+
Sbjct: 442 KAELAGENALERYDGGAYEQILATSRSESGNGLTAFTYLRMNKNLFEPNNWRNLVEFVKT 501

Query: 421 MSEGGRSSRLSESDSSGSDLHVRFIKEKKIKEVAM 455
           MSEGGR+ +L ESD SGSDLHV FIKEKKIKEVA+
Sbjct: 502 MSEGGRNRKLPESDCSGSDLHVGFIKEKKIKEVAV 536

BLAST of Moc10g28230 vs. ExPASy Swiss-Prot
Match: O23553 (Beta-amylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BAM3 PE=1 SV=3)

HSP 1 Score: 769.6 bits (1986), Expect = 2.0e-221
Identity = 355/455 (78.02%), Postives = 399/455 (87.69%), Query Frame = 0

Query: 1   MLPLDTVSVGGSLNKARAMNASLMALKSAGVEGVMVDAWWGLVEKDGPLKYNWEGYAELV 60
           MLPLDTV++ G LNK RAMNASLMALK AGVEGVMVDAWWGLVEKDGP+ YNWEGYAEL+
Sbjct: 91  MLPLDTVTMSGHLNKPRAMNASLMALKGAGVEGVMVDAWWGLVEKDGPMNYNWEGYAELI 150

Query: 61  QMVQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRRNPEYIS 120
           QMVQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTD+SGRRNPEYIS
Sbjct: 151 QMVQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDKSGRRNPEYIS 210

Query: 121 LGCDSLPVLKGRTPIQVYADYMRSFRDRFRDYLGEVIVEIQVGAGPCGELRYPSYPESNG 180
           LGCDS+PVL+GRTPIQVY+D+MRSFR+RF  Y+G VI EIQVG GPCGELRYPSYPESNG
Sbjct: 211 LGCDSVPVLRGRTPIQVYSDFMRSFRERFEGYIGGVIAEIQVGMGPCGELRYPSYPESNG 270

Query: 181 TWRFPGIGEFQCYDKYMRASLEAAAEAAGKREWGSNGPHDSGQYNQFPEDTGFFKRDGTW 240
           TWRFPGIGEFQCYDKYM++SL+A AE+ GK  WG++GPHD+G+Y   PEDT FF+RDGTW
Sbjct: 271 TWRFPGIGEFQCYDKYMKSSLQAYAESIGKTNWGTSGPHDAGEYKNLPEDTEFFRRDGTW 330

Query: 241 KSEYGKFFLEWYSGKLLQHGDMILAAAKGIFQGTGAKLSAKVAGIHWHYQTQSHAAELTA 300
            SEYGKFF+EWYSGKLL+HGD +L++AKGIFQG+GAKLS KVAGIHWHY T+SHAAELTA
Sbjct: 331 NSEYGKFFMEWYSGKLLEHGDQLLSSAKGIFQGSGAKLSGKVAGIHWHYNTRSHAAELTA 390

Query: 301 GYYNTRHRDGYSPIAKMLAKHGAVFNFTCMEMKDGQQPSHGNCSPEGLVAQVKMVTKDTK 360
           GYYNTR+ DGY PIAKM  KHG V NFTCMEMKDG+QP H NCSPEGLV QV+  T+   
Sbjct: 391 GYYNTRNHDGYLPIAKMFNKHGVVLNFTCMEMKDGEQPEHANCSPEGLVKQVQNATRQAG 450

Query: 361 TELAGENALERYDGGAYHQILATSRSESGNGLAAFTYLRMNKNLFEPHNWRNLVEFVKSM 420
           TELAGENALERYD  A+ Q++AT+RS+SGNGL AFTYLRMNK LFE  NW+ LVEFVK+M
Sbjct: 451 TELAGENALERYDSSAFGQVVATNRSDSGNGLTAFTYLRMNKRLFEGQNWQQLVEFVKNM 510

Query: 421 SEGGRSSRLSESDSSGSDLHVRFIKEKKIKEVAMA 456
            EGG   RLS+ D++GSDL+V F+K K  + V  A
Sbjct: 511 KEGGHGRRLSKEDTTGSDLYVGFVKGKIAENVEEA 545

BLAST of Moc10g28230 vs. ExPASy Swiss-Prot
Match: Q9LIR6 (Beta-amylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BAM1 PE=1 SV=1)

HSP 1 Score: 589.7 bits (1519), Expect = 2.8e-167
Identity = 283/463 (61.12%), Postives = 345/463 (74.51%), Query Frame = 0

Query: 1   MLPLDTVSVGGSLNKARAMNASLMALKSAGVEGVMVDAWWGLVEKDGPLKYNWEGYAELV 60
           M+PLD+V++G ++N+ +AM ASL ALKSAGVEG+M+D WWGLVEK+ P  YNW GY EL+
Sbjct: 111 MMPLDSVTMGNTVNRRKAMKASLQALKSAGVEGIMIDVWWGLVEKESPGTYNWGGYNELL 170

Query: 61  QMVQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRRNPEYIS 120
           ++ +K GLK+Q VMSFHQCGGNVGDS +IPLP WV+EE+ K+PDL YTD+ GRRN EYIS
Sbjct: 171 ELAKKLGLKVQAVMSFHQCGGNVGDSVTIPLPQWVVEEVDKDPDLAYTDQWGRRNHEYIS 230

Query: 121 LGCDSLPVLKGRTPIQVYADYMRSFRDRFRDYLGEVIVEIQVGAGPCGELRYPSYPESNG 180
           LG D+LPVLKGRTP+Q YAD+MR+FRD F+  LGE IVEIQVG GP GELRYPSYPE  G
Sbjct: 231 LGADTLPVLKGRTPVQCYADFMRAFRDNFKHLLGETIVEIQVGMGPAGELRYPSYPEQEG 290

Query: 181 TWRFPGIGEFQCYDKYMRASLEAAAEAAGKREWGSNGPHDSGQYNQFPEDTGFFKRD-GT 240
           TW+FPGIG FQCYDKY  +SL+AAAE  GK EWGS GP D+G YN +PEDT FFK++ G 
Sbjct: 291 TWKFPGIGAFQCYDKYSLSSLKAAAETYGKPEWGSTGPTDAGHYNNWPEDTQFFKKEGGG 350

Query: 241 WKSEYGKFFLEWYSGKLLQHGDMILAAAKGIFQGTGAKLSAKVAGIHWHYQTQSHAAELT 300
           W SEYG FFL WYS  LL HG+ IL++AK IF+  G K+S K+AGIHWHY T+SHA ELT
Sbjct: 351 WNSEYGDFFLSWYSQMLLDHGERILSSAKSIFENMGVKISVKIAGIHWHYGTRSHAPELT 410

Query: 301 AGYYNTRHRDGYSPIAKMLAKHGAVFNFTCMEMKDGQQPSHGNCSPEGLVAQVKMVTKDT 360
           AGYYNTR RDGY PIA+MLA+H A+FNFTC+EM+D +QP    C+PE LV QV + T   
Sbjct: 411 AGYYNTRFRDGYLPIAQMLARHNAIFNFTCIEMRDHEQPQDALCAPEKLVNQVALATLAA 470

Query: 361 KTELAGENALERYDGGAYHQIL---ATSRSESGNG----LAAFTYLRMNKNLFEPHNWRN 420
           +  LAGENAL RYD  A+ QIL   A +  ++  G    + AFTYLRMN  LF+  NW  
Sbjct: 471 EVPLAGENALPRYDDYAHEQILKASALNLDQNNEGEPREMCAFTYLRMNPELFQADNWGK 530

Query: 421 LVEFVKSMSEGGRSSRLSESDSSGSDLHVRFIKEKKIKEVAMA 456
            V FVK M EG  S R  E     ++ H   + +  ++E A+A
Sbjct: 531 FVAFVKKMGEGRDSHRCREEVEREAE-HFVHVTQPLVQEAAVA 572

BLAST of Moc10g28230 vs. ExPASy Swiss-Prot
Match: Q9AV88 (Beta-amylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=BAMY1 PE=1 SV=1)

HSP 1 Score: 589.3 bits (1518), Expect = 3.6e-167
Identity = 278/440 (63.18%), Postives = 338/440 (76.82%), Query Frame = 0

Query: 1   MLPLDTVS-VGGSLNKARAMNASLMALKSAGVEGVMVDAWWGLVEKDGPLKYNWEGYAEL 60
           M+PLDTVS  G +LN+ +A+ ASL ALKSAGVEG+MVD WWG+VE +GP +YN++GY EL
Sbjct: 78  MMPLDTVSKCGSALNRRKAVAASLAALKSAGVEGIMVDVWWGIVESEGPGRYNFDGYVEL 137

Query: 61  VQMVQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRRNPEYI 120
           ++M +K GLK+Q VMSFHQCGGNVGDS +IPLP WV+EE+ K+ DL YTD+ GRRN EYI
Sbjct: 138 MEMARKTGLKVQAVMSFHQCGGNVGDSVNIPLPRWVVEEMEKDNDLAYTDQWGRRNFEYI 197

Query: 121 SLGCDSLPVLKGRTPIQVYADYMRSFRDRFRDYLGEVIVEIQVGAGPCGELRYPSYPESN 180
           SLGCD++PV KGRTP++ Y D+MR+FRD F  +LG+ IVEIQVG GP GELRYPSYPESN
Sbjct: 198 SLGCDAMPVFKGRTPVECYTDFMRAFRDHFASFLGDTIVEIQVGMGPAGELRYPSYPESN 257

Query: 181 GTWRFPGIGEFQCYDKYMRASLEAAAEAAGKREWGSNGPHDSGQYNQFPEDTGFFKRD-G 240
           GTWRFPGIG FQC D+YMR+SL+AAAEA GK EWG  GP D+G YN +PEDT FF+ D G
Sbjct: 258 GTWRFPGIGAFQCNDRYMRSSLKAAAEARGKPEWGHGGPTDAGGYNNWPEDTVFFRGDCG 317

Query: 241 TWKSEYGKFFLEWYSGKLLQHGDMILAAAKGIF-QGTGAKLSAKVAGIHWHYQTQSHAAE 300
            W +EYG+FFL WYS  LL+HG+ +L+ A  +F  G GAK+S KVAGIHWHY T+SHA E
Sbjct: 318 GWSTEYGEFFLSWYSQMLLEHGERVLSGATSVFGDGAGAKISVKVAGIHWHYGTRSHAPE 377

Query: 301 LTAGYYNTRHRDGYSPIAKMLAKHGAVFNFTCMEMKDGQQPSHGNCSPEGLVAQVKMVTK 360
           LTAGYYNTRHRDGY PIA+MLA+HGAV NFTC+EM+D +QP    C PE LV QV    +
Sbjct: 378 LTAGYYNTRHRDGYLPIARMLARHGAVLNFTCVEMRDHEQPQEAQCMPEALVRQVAAAAR 437

Query: 361 DTKTELAGENALERYDGGAYHQILATSRSESG-NGLAAFTYLRMNKNLFEPHNWRNLVEF 420
                LAGENAL RYDG A+ Q++A +   +  + + AFTYLRM  +LF P NWR  V F
Sbjct: 438 AAGVGLAGENALPRYDGTAHDQVVAAAADRAAEDRMVAFTYLRMGPDLFHPDNWRRFVAF 497

Query: 421 VKSMSEGGRSSRLSESDSSG 437
           V+ MSE G     +ES + G
Sbjct: 498 VRRMSESGSPREAAESAAHG 517

BLAST of Moc10g28230 vs. ExPASy Swiss-Prot
Match: Q10RZ1 (Beta-amylase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=BAMY2 PE=1 SV=1)

HSP 1 Score: 588.2 bits (1515), Expect = 8.1e-167
Identity = 273/427 (63.93%), Postives = 334/427 (78.22%), Query Frame = 0

Query: 1   MLPLDTV-SVGGSLNKARAMNASLMALKSAGVEGVMVDAWWGLVEKDGPLKYNWEGYAEL 60
           M+PLDTV   G  LN+ +A+ ASL ALKSAG EG+MVD WWG+ E +GP +YN+ GY EL
Sbjct: 98  MMPLDTVRKDGNGLNRRKAVEASLKALKSAGAEGIMVDVWWGIAECEGPGRYNFTGYMEL 157

Query: 61  VQMVQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRRNPEYI 120
           ++M +K+GLK+Q VMSFHQCGGNVGDS +IPLP WVLEE+ K+ DL YTDRSGRRN EY+
Sbjct: 158 MEMAKKNGLKVQAVMSFHQCGGNVGDSVTIPLPKWVLEEMDKDQDLAYTDRSGRRNYEYL 217

Query: 121 SLGCDSLPVLKGRTPIQVYADYMRSFRDRFRDYLGEVIVEIQVGAGPCGELRYPSYPESN 180
           SLG D++PVLKGRTP+Q Y D+MR+FRD F  ++G  IVEIQVG GP GELRYPSYPESN
Sbjct: 218 SLGADAMPVLKGRTPVQCYGDFMRAFRDHFAAFMGNTIVEIQVGMGPAGELRYPSYPESN 277

Query: 181 GTWRFPGIGEFQCYDKYMRASLEAAAEAAGKREWGSNGPHDSGQYNQFPEDTGFFKRDGT 240
           GTWRFPGIGEFQCYD+YM +SL+AAAEA GK EWG+ GP DSG YN +PED+ FF+R+G 
Sbjct: 278 GTWRFPGIGEFQCYDRYMLSSLKAAAEAVGKPEWGNAGPGDSGGYNDWPEDSPFFRREGG 337

Query: 241 WKSEYGKFFLEWYSGKLLQHGDMILAAAKGIFQGT-GAKLSAKVAGIHWHYQTQSHAAEL 300
           W + YG+FF+ WYS  LL+HG+ IL+AA G++ GT G K+S KVAGIHWHY T+SHAAEL
Sbjct: 338 WNTPYGEFFMSWYSQMLLEHGERILSAASGVYTGTPGVKISVKVAGIHWHYGTRSHAAEL 397

Query: 301 TAGYYNTRHRDGYSPIAKMLAKHGAVFNFTCMEMKDGQQPSHGNCSPEGLVAQVKMVTKD 360
           TAGYYNTRH DGY PIA+MLA+HGAV NFTC+EM++ +QP    C PE LV QV    ++
Sbjct: 398 TAGYYNTRHHDGYQPIARMLARHGAVLNFTCVEMRNHEQPQDAQCRPEELVQQVAAAARE 457

Query: 361 TKTELAGENALERYDGGAYHQILATSRSES-GNGLAAFTYLRMNKNLFEPHNWRNLVEFV 420
           +   LAGENAL RYD  A+ QI+ T+  ++    + AFTYLRM  +LF+P NWR    FV
Sbjct: 458 SGVGLAGENALPRYDETAHDQIVTTAAEKAEEERMVAFTYLRMGPDLFQPDNWRRFAAFV 517

Query: 421 KSMSEGG 425
           K M+E G
Sbjct: 518 KRMTESG 524

BLAST of Moc10g28230 vs. ExPASy Swiss-Prot
Match: O22585 (Beta-amylase OS=Medicago sativa OX=3879 GN=BMY1 PE=2 SV=1)

HSP 1 Score: 461.8 bits (1187), Expect = 8.7e-129
Identity = 223/428 (52.10%), Postives = 291/428 (67.99%), Query Frame = 0

Query: 1   MLPLDTVSVGGSLNKARAMNASLMALKSAGVEGVMVDAWWGLVEKDGPLKYNWEGYAELV 60
           MLPL  ++V         +   L+ L++AGV+GVM+D WWG++E+ GP +Y+W  Y  L 
Sbjct: 18  MLPLGVINVNNVFEDPDGLKEQLVQLRAAGVDGVMIDVWWGIIEQKGPKEYDWSAYKSLF 77

Query: 61  QMVQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRRNPEYIS 120
           Q+VQK GLKLQ +MSFHQCGGNVGD  +IPLP WVL+    +PD+ YT+RSG RN EY+S
Sbjct: 78  QLVQKCGLKLQAIMSFHQCGGNVGDVVNIPLPKWVLDIGESDPDIFYTNRSGIRNQEYLS 137

Query: 121 LGCDSLPVLKGRTPIQVYADYMRSFRDRFRDYL-GEVIVEIQVGAGPCGELRYPSYPESN 180
           +G D+ P+  GRT I++Y+DYM+SFR+   D L  EVI++I+VG GP GELRYPSYP++ 
Sbjct: 138 IGVDNKPIFHGRTAIEIYSDYMKSFRENMSDLLKSEVIIDIEVGLGPAGELRYPSYPQNQ 197

Query: 181 GTWRFPGIGEFQCYDKYMRASLEAAAEAAGKREWGSNGPHDSGQYNQFPEDTGFFKRDGT 240
           G W+FPGIGEFQCYDKY+R S +AAA  AG  EW    P D+G YN  PE T FFK +GT
Sbjct: 198 G-WQFPGIGEFQCYDKYLRESFKAAAAKAGHSEW--ELPDDAGTYNDVPESTEFFKTNGT 257

Query: 241 WKSEYGKFFLEWYSGKLLQHGDMILAAAKGIFQGTGAKLSAKVAGIHWHYQTQSHAAELT 300
           + +E GKFFL WYS +LL HGD IL  A   F G   KL+ KV+GIHW Y+  +HAAELT
Sbjct: 258 YLTEKGKFFLTWYSNQLLNHGDQILDEANKAFLGCKVKLAIKVSGIHWWYKAPNHAAELT 317

Query: 301 AGYYNTRHRDGYSPIAKMLAKHGAVFNFTCMEMKDGQQPSHGNCSPEGLVAQVKMVTKDT 360
           AGYYN   RDGY PIAK++++H A+ NFTC+EM+D +Q S  + SP+ LV QV       
Sbjct: 318 AGYYNLDDRDGYRPIAKIVSRHHAILNFTCLEMRDSEQSSDAHSSPQKLVQQVLSGGWRE 377

Query: 361 KTELAGENALERYDGGAYHQILATSRSESGN-------GLAAFTYLRMNKNLFEPHNWRN 420
             E+AGENAL RYD  AY+QI+  +R +  N        +   TYLR++ +L +  N+  
Sbjct: 378 NIEVAGENALSRYDATAYNQIILNARPQGVNKDGPPKLRMYGVTYLRLSDDLMQQSNFDI 437

BLAST of Moc10g28230 vs. ExPASy TrEMBL
Match: A0A6J1DRU1 (Beta-amylase OS=Momordica charantia OX=3673 GN=LOC111022628 PE=3 SV=1)

HSP 1 Score: 944.9 bits (2441), Expect = 1.3e-271
Identity = 455/455 (100.00%), Postives = 455/455 (100.00%), Query Frame = 0

Query: 1   MLPLDTVSVGGSLNKARAMNASLMALKSAGVEGVMVDAWWGLVEKDGPLKYNWEGYAELV 60
           MLPLDTVSVGGSLNKARAMNASLMALKSAGVEGVMVDAWWGLVEKDGPLKYNWEGYAELV
Sbjct: 87  MLPLDTVSVGGSLNKARAMNASLMALKSAGVEGVMVDAWWGLVEKDGPLKYNWEGYAELV 146

Query: 61  QMVQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRRNPEYIS 120
           QMVQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRRNPEYIS
Sbjct: 147 QMVQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRRNPEYIS 206

Query: 121 LGCDSLPVLKGRTPIQVYADYMRSFRDRFRDYLGEVIVEIQVGAGPCGELRYPSYPESNG 180
           LGCDSLPVLKGRTPIQVYADYMRSFRDRFRDYLGEVIVEIQVGAGPCGELRYPSYPESNG
Sbjct: 207 LGCDSLPVLKGRTPIQVYADYMRSFRDRFRDYLGEVIVEIQVGAGPCGELRYPSYPESNG 266

Query: 181 TWRFPGIGEFQCYDKYMRASLEAAAEAAGKREWGSNGPHDSGQYNQFPEDTGFFKRDGTW 240
           TWRFPGIGEFQCYDKYMRASLEAAAEAAGKREWGSNGPHDSGQYNQFPEDTGFFKRDGTW
Sbjct: 267 TWRFPGIGEFQCYDKYMRASLEAAAEAAGKREWGSNGPHDSGQYNQFPEDTGFFKRDGTW 326

Query: 241 KSEYGKFFLEWYSGKLLQHGDMILAAAKGIFQGTGAKLSAKVAGIHWHYQTQSHAAELTA 300
           KSEYGKFFLEWYSGKLLQHGDMILAAAKGIFQGTGAKLSAKVAGIHWHYQTQSHAAELTA
Sbjct: 327 KSEYGKFFLEWYSGKLLQHGDMILAAAKGIFQGTGAKLSAKVAGIHWHYQTQSHAAELTA 386

Query: 301 GYYNTRHRDGYSPIAKMLAKHGAVFNFTCMEMKDGQQPSHGNCSPEGLVAQVKMVTKDTK 360
           GYYNTRHRDGYSPIAKMLAKHGAVFNFTCMEMKDGQQPSHGNCSPEGLVAQVKMVTKDTK
Sbjct: 387 GYYNTRHRDGYSPIAKMLAKHGAVFNFTCMEMKDGQQPSHGNCSPEGLVAQVKMVTKDTK 446

Query: 361 TELAGENALERYDGGAYHQILATSRSESGNGLAAFTYLRMNKNLFEPHNWRNLVEFVKSM 420
           TELAGENALERYDGGAYHQILATSRSESGNGLAAFTYLRMNKNLFEPHNWRNLVEFVKSM
Sbjct: 447 TELAGENALERYDGGAYHQILATSRSESGNGLAAFTYLRMNKNLFEPHNWRNLVEFVKSM 506

Query: 421 SEGGRSSRLSESDSSGSDLHVRFIKEKKIKEVAMA 456
           SEGGRSSRLSESDSSGSDLHVRFIKEKKIKEVAMA
Sbjct: 507 SEGGRSSRLSESDSSGSDLHVRFIKEKKIKEVAMA 541

BLAST of Moc10g28230 vs. ExPASy TrEMBL
Match: A0A5D3CKL1 (Beta-amylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1017G00910 PE=3 SV=1)

HSP 1 Score: 872.5 bits (2253), Expect = 7.9e-250
Identity = 413/453 (91.17%), Postives = 433/453 (95.58%), Query Frame = 0

Query: 1   MLPLDTVSVGGSLNKARAMNASLMALKSAGVEGVMVDAWWGLVEKDGPLKYNWEGYAELV 60
           MLPLDTV++GGSLNK RAMNASLMALKSAGVEGVMVDAWWGLVEKDGP+KYNWEGYAELV
Sbjct: 83  MLPLDTVTLGGSLNKPRAMNASLMALKSAGVEGVMVDAWWGLVEKDGPMKYNWEGYAELV 142

Query: 61  QMVQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRRNPEYIS 120
           QMVQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRRNPEYIS
Sbjct: 143 QMVQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRRNPEYIS 202

Query: 121 LGCDSLPVLKGRTPIQVYADYMRSFRDRFRDYLGEVIVEIQVGAGPCGELRYPSYPESNG 180
           LGCDSLPVL+GRTPIQVYADYMRSFRDRFRDYLGEVI+E+QVGAGPCGELRYPSYPESNG
Sbjct: 203 LGCDSLPVLRGRTPIQVYADYMRSFRDRFRDYLGEVIMEVQVGAGPCGELRYPSYPESNG 262

Query: 181 TWRFPGIGEFQCYDKYMRASLEAAAEAAGKREWGSNGPHDSGQYNQFPEDTGFFKRDGTW 240
           TWRFPGIGEFQCYDKYMRASLEAAAEA GKR+WGS+GPHDSGQYNQFPEDTGFFK++GTW
Sbjct: 263 TWRFPGIGEFQCYDKYMRASLEAAAEAIGKRDWGSSGPHDSGQYNQFPEDTGFFKKEGTW 322

Query: 241 KSEYGKFFLEWYSGKLLQHGDMILAAAKGIFQGTGAKLSAKVAGIHWHYQTQSHAAELTA 300
           K+EYG+FFL WYS KLLQHGD ILAAAKGIF+GTGAKLSAKVAGIHWHY T+SHAAELTA
Sbjct: 323 KTEYGEFFLAWYSSKLLQHGDSILAAAKGIFRGTGAKLSAKVAGIHWHYGTRSHAAELTA 382

Query: 301 GYYNTRHRDGYSPIAKMLAKHGAVFNFTCMEMKDGQQPSHGNCSPEGLVAQVKMVTKDTK 360
           GYYNTRHRDGYSPIAKMLAKHG VFNFTCMEM+DGQQP + NCSPEGLV QVKM T+D K
Sbjct: 383 GYYNTRHRDGYSPIAKMLAKHGVVFNFTCMEMRDGQQPGYANCSPEGLVRQVKMATRDAK 442

Query: 361 TELAGENALERYDGGAYHQILATSRSESGNGLAAFTYLRMNKNLFEPHNWRNLVEFVKSM 420
            ELAGENALERYDG AY QILATSRS+SGNGLAAFTYLRMNKNLFEP+NWRNLVEFVKSM
Sbjct: 443 VELAGENALERYDGAAYEQILATSRSDSGNGLAAFTYLRMNKNLFEPNNWRNLVEFVKSM 502

Query: 421 SEGGRSSRLSESDSSGSDLHVRFIKEKKIKEVA 454
           SEGGR+ RL ESD  GSDLHV FIKEKKIKEVA
Sbjct: 503 SEGGRNRRLPESDCCGSDLHVGFIKEKKIKEVA 535

BLAST of Moc10g28230 vs. ExPASy TrEMBL
Match: A0A1S3BKF9 (Beta-amylase OS=Cucumis melo OX=3656 GN=LOC103490827 PE=3 SV=1)

HSP 1 Score: 872.5 bits (2253), Expect = 7.9e-250
Identity = 413/453 (91.17%), Postives = 433/453 (95.58%), Query Frame = 0

Query: 1   MLPLDTVSVGGSLNKARAMNASLMALKSAGVEGVMVDAWWGLVEKDGPLKYNWEGYAELV 60
           MLPLDTV++GGSLNK RAMNASLMALKSAGVEGVMVDAWWGLVEKDGP+KYNWEGYAELV
Sbjct: 83  MLPLDTVTLGGSLNKPRAMNASLMALKSAGVEGVMVDAWWGLVEKDGPMKYNWEGYAELV 142

Query: 61  QMVQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRRNPEYIS 120
           QMVQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRRNPEYIS
Sbjct: 143 QMVQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRRNPEYIS 202

Query: 121 LGCDSLPVLKGRTPIQVYADYMRSFRDRFRDYLGEVIVEIQVGAGPCGELRYPSYPESNG 180
           LGCDSLPVL+GRTPIQVYADYMRSFRDRFRDYLGEVI+E+QVGAGPCGELRYPSYPESNG
Sbjct: 203 LGCDSLPVLRGRTPIQVYADYMRSFRDRFRDYLGEVIMEVQVGAGPCGELRYPSYPESNG 262

Query: 181 TWRFPGIGEFQCYDKYMRASLEAAAEAAGKREWGSNGPHDSGQYNQFPEDTGFFKRDGTW 240
           TWRFPGIGEFQCYDKYMRASLEAAAEA GKR+WGS+GPHDSGQYNQFPEDTGFFK++GTW
Sbjct: 263 TWRFPGIGEFQCYDKYMRASLEAAAEAIGKRDWGSSGPHDSGQYNQFPEDTGFFKKEGTW 322

Query: 241 KSEYGKFFLEWYSGKLLQHGDMILAAAKGIFQGTGAKLSAKVAGIHWHYQTQSHAAELTA 300
           K+EYG+FFL WYS KLLQHGD ILAAAKGIF+GTGAKLSAKVAGIHWHY T+SHAAELTA
Sbjct: 323 KTEYGEFFLAWYSSKLLQHGDSILAAAKGIFRGTGAKLSAKVAGIHWHYGTRSHAAELTA 382

Query: 301 GYYNTRHRDGYSPIAKMLAKHGAVFNFTCMEMKDGQQPSHGNCSPEGLVAQVKMVTKDTK 360
           GYYNTRHRDGYSPIAKMLAKHG VFNFTCMEM+DGQQP + NCSPEGLV QVKM T+D K
Sbjct: 383 GYYNTRHRDGYSPIAKMLAKHGVVFNFTCMEMRDGQQPGYANCSPEGLVRQVKMATRDAK 442

Query: 361 TELAGENALERYDGGAYHQILATSRSESGNGLAAFTYLRMNKNLFEPHNWRNLVEFVKSM 420
            ELAGENALERYDG AY QILATSRS+SGNGLAAFTYLRMNKNLFEP+NWRNLVEFVKSM
Sbjct: 443 VELAGENALERYDGAAYEQILATSRSDSGNGLAAFTYLRMNKNLFEPNNWRNLVEFVKSM 502

Query: 421 SEGGRSSRLSESDSSGSDLHVRFIKEKKIKEVA 454
           SEGGR+ RL ESD  GSDLHV FIKEKKIKEVA
Sbjct: 503 SEGGRNRRLPESDCCGSDLHVGFIKEKKIKEVA 535

BLAST of Moc10g28230 vs. ExPASy TrEMBL
Match: A0A0A0L215 (Beta-amylase OS=Cucumis sativus OX=3659 GN=Csa_3G017020 PE=3 SV=1)

HSP 1 Score: 869.8 bits (2246), Expect = 5.1e-249
Identity = 414/454 (91.19%), Postives = 432/454 (95.15%), Query Frame = 0

Query: 1   MLPLDTVSVGGSLNKARAMNASLMALKSAGVEGVMVDAWWGLVEKDGPLKYNWEGYAELV 60
           MLPLDTV++GGSLNK RAMNASLMALKSAGVEGVMVDAWWGLVEKDGP+KYNWEGYAELV
Sbjct: 83  MLPLDTVTLGGSLNKPRAMNASLMALKSAGVEGVMVDAWWGLVEKDGPMKYNWEGYAELV 142

Query: 61  QMVQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRRNPEYIS 120
           QMVQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRRNPEYIS
Sbjct: 143 QMVQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRRNPEYIS 202

Query: 121 LGCDSLPVLKGRTPIQVYADYMRSFRDRFRDYLGEVIVEIQVGAGPCGELRYPSYPESNG 180
           LGCDSLPVL+GRTPIQVYADYMRSFRDRFRDYLGEVI E+QVGAGPCGELRYPSYPESNG
Sbjct: 203 LGCDSLPVLRGRTPIQVYADYMRSFRDRFRDYLGEVITEVQVGAGPCGELRYPSYPESNG 262

Query: 181 TWRFPGIGEFQCYDKYMRASLEAAAEAAGKREWGSN-GPHDSGQYNQFPEDTGFFKRDGT 240
           TWRFPGIGEFQCYDKYMRASLEAAAEA GKR+WGS+ GPHDSGQYNQFPEDTGFFK++GT
Sbjct: 263 TWRFPGIGEFQCYDKYMRASLEAAAEAIGKRDWGSSGGPHDSGQYNQFPEDTGFFKKEGT 322

Query: 241 WKSEYGKFFLEWYSGKLLQHGDMILAAAKGIFQGTGAKLSAKVAGIHWHYQTQSHAAELT 300
           WK+EYG+FFL WYS KLLQHGD ILAAAKGIF+GTGAKLSAKVAGIHWHY T+SHAAELT
Sbjct: 323 WKTEYGEFFLAWYSSKLLQHGDSILAAAKGIFRGTGAKLSAKVAGIHWHYGTRSHAAELT 382

Query: 301 AGYYNTRHRDGYSPIAKMLAKHGAVFNFTCMEMKDGQQPSHGNCSPEGLVAQVKMVTKDT 360
           AGYYNTRHRDGYSPIAKMLAKHG VFNFTCMEM+DGQQP H NCSPEGLV QVKM T+D 
Sbjct: 383 AGYYNTRHRDGYSPIAKMLAKHGVVFNFTCMEMRDGQQPGHANCSPEGLVRQVKMATRDA 442

Query: 361 KTELAGENALERYDGGAYHQILATSRSESGNGLAAFTYLRMNKNLFEPHNWRNLVEFVKS 420
           K ELAGENALERYDG AY QILATSRS+SGNGLAAFTYLRMNKNLFEP+NWRNLVEFVKS
Sbjct: 443 KVELAGENALERYDGAAYEQILATSRSDSGNGLAAFTYLRMNKNLFEPNNWRNLVEFVKS 502

Query: 421 MSEGGRSSRLSESDSSGSDLHVRFIKEKKIKEVA 454
           MSEGGR+ RL ESD  GSDLHV FIKEKKIKEVA
Sbjct: 503 MSEGGRNRRLPESDCCGSDLHVGFIKEKKIKEVA 536

BLAST of Moc10g28230 vs. ExPASy TrEMBL
Match: A0A6J1L3X7 (Beta-amylase OS=Cucurbita maxima OX=3661 GN=LOC111500288 PE=3 SV=1)

HSP 1 Score: 869.0 bits (2244), Expect = 8.7e-249
Identity = 411/455 (90.33%), Postives = 435/455 (95.60%), Query Frame = 0

Query: 1   MLPLDTVSVGGSLNKARAMNASLMALKSAGVEGVMVDAWWGLVEKDGPLKYNWEGYAELV 60
           MLPLDTV++GG+LNK RAMNASLMALKSAGVEGVMVDAWWGLVEKDGP+KYNWEGYAELV
Sbjct: 82  MLPLDTVTLGGNLNKPRAMNASLMALKSAGVEGVMVDAWWGLVEKDGPMKYNWEGYAELV 141

Query: 61  QMVQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRRNPEYIS 120
           QM+QKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRRNPEYIS
Sbjct: 142 QMLQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRRNPEYIS 201

Query: 121 LGCDSLPVLKGRTPIQVYADYMRSFRDRFRDYLGEVIVEIQVGAGPCGELRYPSYPESNG 180
           LGCDSLPVL+GRTPIQVYADYMRSFRDRFRDYLGEVI+E+QVGAGPCGELRYPSYP+SNG
Sbjct: 202 LGCDSLPVLRGRTPIQVYADYMRSFRDRFRDYLGEVIMEVQVGAGPCGELRYPSYPQSNG 261

Query: 181 TWRFPGIGEFQCYDKYMRASLEAAAEAAGKREWGSN-GPHDSGQYNQFPEDTGFFKRDGT 240
           TWRFPGIGEFQCYDKYMRASLEAAAE+ GKR+WGSN GPHDSGQYNQFPEDTGFFK++GT
Sbjct: 262 TWRFPGIGEFQCYDKYMRASLEAAAESIGKRDWGSNGGPHDSGQYNQFPEDTGFFKKEGT 321

Query: 241 WKSEYGKFFLEWYSGKLLQHGDMILAAAKGIFQGTGAKLSAKVAGIHWHYQTQSHAAELT 300
           W +EYGKFFLEWYSGKLLQHGD ILAA+KGIF GTGAKLSAKVAGIHWHY T+SHAAELT
Sbjct: 322 WNTEYGKFFLEWYSGKLLQHGDRILAASKGIFHGTGAKLSAKVAGIHWHYGTRSHAAELT 381

Query: 301 AGYYNTRHRDGYSPIAKMLAKHGAVFNFTCMEMKDGQQPSHGNCSPEGLVAQVKMVTKDT 360
           AGYYNTRHR+GYSPIAKMLAKHG VFNFTCMEMKDGQQP + NCSPEGLV QVKM T+D 
Sbjct: 382 AGYYNTRHRNGYSPIAKMLAKHGVVFNFTCMEMKDGQQPGYANCSPEGLVRQVKMATRDA 441

Query: 361 KTELAGENALERYDGGAYHQILATSRSESGNGLAAFTYLRMNKNLFEPHNWRNLVEFVKS 420
           K ELAGENALERYDGGAY QILATSRSESGNGL AFTYLRMNKNLFEP+NWRNLVEFVK+
Sbjct: 442 KAELAGENALERYDGGAYEQILATSRSESGNGLTAFTYLRMNKNLFEPNNWRNLVEFVKT 501

Query: 421 MSEGGRSSRLSESDSSGSDLHVRFIKEKKIKEVAM 455
           MSEGGR+ +L ESD SGSDLHV FIKEKKIKEVA+
Sbjct: 502 MSEGGRNRKLPESDCSGSDLHVGFIKEKKIKEVAV 536

BLAST of Moc10g28230 vs. TAIR 10
Match: AT4G17090.1 (chloroplast beta-amylase )

HSP 1 Score: 769.6 bits (1986), Expect = 1.4e-222
Identity = 355/455 (78.02%), Postives = 399/455 (87.69%), Query Frame = 0

Query: 1   MLPLDTVSVGGSLNKARAMNASLMALKSAGVEGVMVDAWWGLVEKDGPLKYNWEGYAELV 60
           MLPLDTV++ G LNK RAMNASLMALK AGVEGVMVDAWWGLVEKDGP+ YNWEGYAEL+
Sbjct: 91  MLPLDTVTMSGHLNKPRAMNASLMALKGAGVEGVMVDAWWGLVEKDGPMNYNWEGYAELI 150

Query: 61  QMVQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRRNPEYIS 120
           QMVQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTD+SGRRNPEYIS
Sbjct: 151 QMVQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDKSGRRNPEYIS 210

Query: 121 LGCDSLPVLKGRTPIQVYADYMRSFRDRFRDYLGEVIVEIQVGAGPCGELRYPSYPESNG 180
           LGCDS+PVL+GRTPIQVY+D+MRSFR+RF  Y+G VI EIQVG GPCGELRYPSYPESNG
Sbjct: 211 LGCDSVPVLRGRTPIQVYSDFMRSFRERFEGYIGGVIAEIQVGMGPCGELRYPSYPESNG 270

Query: 181 TWRFPGIGEFQCYDKYMRASLEAAAEAAGKREWGSNGPHDSGQYNQFPEDTGFFKRDGTW 240
           TWRFPGIGEFQCYDKYM++SL+A AE+ GK  WG++GPHD+G+Y   PEDT FF+RDGTW
Sbjct: 271 TWRFPGIGEFQCYDKYMKSSLQAYAESIGKTNWGTSGPHDAGEYKNLPEDTEFFRRDGTW 330

Query: 241 KSEYGKFFLEWYSGKLLQHGDMILAAAKGIFQGTGAKLSAKVAGIHWHYQTQSHAAELTA 300
            SEYGKFF+EWYSGKLL+HGD +L++AKGIFQG+GAKLS KVAGIHWHY T+SHAAELTA
Sbjct: 331 NSEYGKFFMEWYSGKLLEHGDQLLSSAKGIFQGSGAKLSGKVAGIHWHYNTRSHAAELTA 390

Query: 301 GYYNTRHRDGYSPIAKMLAKHGAVFNFTCMEMKDGQQPSHGNCSPEGLVAQVKMVTKDTK 360
           GYYNTR+ DGY PIAKM  KHG V NFTCMEMKDG+QP H NCSPEGLV QV+  T+   
Sbjct: 391 GYYNTRNHDGYLPIAKMFNKHGVVLNFTCMEMKDGEQPEHANCSPEGLVKQVQNATRQAG 450

Query: 361 TELAGENALERYDGGAYHQILATSRSESGNGLAAFTYLRMNKNLFEPHNWRNLVEFVKSM 420
           TELAGENALERYD  A+ Q++AT+RS+SGNGL AFTYLRMNK LFE  NW+ LVEFVK+M
Sbjct: 451 TELAGENALERYDSSAFGQVVATNRSDSGNGLTAFTYLRMNKRLFEGQNWQQLVEFVKNM 510

Query: 421 SEGGRSSRLSESDSSGSDLHVRFIKEKKIKEVAMA 456
            EGG   RLS+ D++GSDL+V F+K K  + V  A
Sbjct: 511 KEGGHGRRLSKEDTTGSDLYVGFVKGKIAENVEEA 545

BLAST of Moc10g28230 vs. TAIR 10
Match: AT3G23920.1 (beta-amylase 1 )

HSP 1 Score: 589.7 bits (1519), Expect = 2.0e-168
Identity = 283/463 (61.12%), Postives = 345/463 (74.51%), Query Frame = 0

Query: 1   MLPLDTVSVGGSLNKARAMNASLMALKSAGVEGVMVDAWWGLVEKDGPLKYNWEGYAELV 60
           M+PLD+V++G ++N+ +AM ASL ALKSAGVEG+M+D WWGLVEK+ P  YNW GY EL+
Sbjct: 111 MMPLDSVTMGNTVNRRKAMKASLQALKSAGVEGIMIDVWWGLVEKESPGTYNWGGYNELL 170

Query: 61  QMVQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRRNPEYIS 120
           ++ +K GLK+Q VMSFHQCGGNVGDS +IPLP WV+EE+ K+PDL YTD+ GRRN EYIS
Sbjct: 171 ELAKKLGLKVQAVMSFHQCGGNVGDSVTIPLPQWVVEEVDKDPDLAYTDQWGRRNHEYIS 230

Query: 121 LGCDSLPVLKGRTPIQVYADYMRSFRDRFRDYLGEVIVEIQVGAGPCGELRYPSYPESNG 180
           LG D+LPVLKGRTP+Q YAD+MR+FRD F+  LGE IVEIQVG GP GELRYPSYPE  G
Sbjct: 231 LGADTLPVLKGRTPVQCYADFMRAFRDNFKHLLGETIVEIQVGMGPAGELRYPSYPEQEG 290

Query: 181 TWRFPGIGEFQCYDKYMRASLEAAAEAAGKREWGSNGPHDSGQYNQFPEDTGFFKRD-GT 240
           TW+FPGIG FQCYDKY  +SL+AAAE  GK EWGS GP D+G YN +PEDT FFK++ G 
Sbjct: 291 TWKFPGIGAFQCYDKYSLSSLKAAAETYGKPEWGSTGPTDAGHYNNWPEDTQFFKKEGGG 350

Query: 241 WKSEYGKFFLEWYSGKLLQHGDMILAAAKGIFQGTGAKLSAKVAGIHWHYQTQSHAAELT 300
           W SEYG FFL WYS  LL HG+ IL++AK IF+  G K+S K+AGIHWHY T+SHA ELT
Sbjct: 351 WNSEYGDFFLSWYSQMLLDHGERILSSAKSIFENMGVKISVKIAGIHWHYGTRSHAPELT 410

Query: 301 AGYYNTRHRDGYSPIAKMLAKHGAVFNFTCMEMKDGQQPSHGNCSPEGLVAQVKMVTKDT 360
           AGYYNTR RDGY PIA+MLA+H A+FNFTC+EM+D +QP    C+PE LV QV + T   
Sbjct: 411 AGYYNTRFRDGYLPIAQMLARHNAIFNFTCIEMRDHEQPQDALCAPEKLVNQVALATLAA 470

Query: 361 KTELAGENALERYDGGAYHQIL---ATSRSESGNG----LAAFTYLRMNKNLFEPHNWRN 420
           +  LAGENAL RYD  A+ QIL   A +  ++  G    + AFTYLRMN  LF+  NW  
Sbjct: 471 EVPLAGENALPRYDDYAHEQILKASALNLDQNNEGEPREMCAFTYLRMNPELFQADNWGK 530

Query: 421 LVEFVKSMSEGGRSSRLSESDSSGSDLHVRFIKEKKIKEVAMA 456
            V FVK M EG  S R  E     ++ H   + +  ++E A+A
Sbjct: 531 FVAFVKKMGEGRDSHRCREEVEREAE-HFVHVTQPLVQEAAVA 572

BLAST of Moc10g28230 vs. TAIR 10
Match: AT4G15210.1 (beta-amylase 5 )

HSP 1 Score: 441.4 bits (1134), Expect = 8.6e-124
Identity = 216/429 (50.35%), Postives = 281/429 (65.50%), Query Frame = 0

Query: 1   MLPLDTVSVGGSLNKARAMNASLMALK-SAGVEGVMVDAWWGLVEKDGPLKYNWEGYAEL 60
           MLPL  V+V         +   L  LK  AGV+GVMVD WWG++E  GP +Y+W  Y  L
Sbjct: 19  MLPLGVVNVENVFADPETLETQLKRLKEEAGVDGVMVDVWWGIIESKGPKQYDWTAYKTL 78

Query: 61  VQMVQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRRNPEYI 120
            Q++ + GLK+Q +MSFHQCGGNVGD  +IP+P WV +    +PD+ YT+R G R+ EY+
Sbjct: 79  FQLIARLGLKIQAIMSFHQCGGNVGDIVTIPIPQWVRDVGDNDPDIYYTNRKGTRDIEYL 138

Query: 121 SLGCDSLPVLKGRTPIQVYADYMRSFRDRFRDYL-GEVIVEIQVGAGPCGELRYPSYPES 180
           S+G D+LP+  GRT +Q+Y+DYM SF++   D +   VIV+I+VG GP GELRYPSYP+S
Sbjct: 139 SIGVDNLPLFAGRTAVQLYSDYMSSFKENMADLIEAGVIVDIEVGLGPAGELRYPSYPQS 198

Query: 181 NGTWRFPGIGEFQCYDKYMRASLEAAAEAAGKREWGSNGPHDSGQYNQFPEDTGFFKRDG 240
            G W FPGIGEFQCYDKY++   + AA  AG  EW  + P D+G+YN  PE+TGFFK+DG
Sbjct: 199 QG-WVFPGIGEFQCYDKYLKKDFKEAAAKAGHPEW--DLPEDAGEYNDKPEETGFFKKDG 258

Query: 241 TWKSEYGKFFLEWYSGKLLQHGDMILAAAKGIFQGTGAKLSAKVAGIHWHYQTQSHAAEL 300
           T+ SE GKFF+ WYS KL+ HGD IL  A  IF G    L+AKV+GIHW Y   SHAAEL
Sbjct: 259 TYVSEKGKFFMTWYSNKLIFHGDQILGEANKIFAGLKVNLAAKVSGIHWLYNHHSHAAEL 318

Query: 301 TAGYYNTRHRDGYSPIAKMLAKHGAVFNFTCMEMKDGQQPSHGNCSPEGLVAQVKMVTKD 360
           TAGYYN   RDGY PIA+ML+KH  + NFTC+EMKD    +    +P+ LV +V      
Sbjct: 319 TAGYYNLFKRDGYRPIARMLSKHYGILNFTCLEMKDTDNTAEALSAPQELVQEVLSKAWK 378

Query: 361 TKTELAGENALERYDGGAYHQILATSRSESGN-------GLAAFTYLRMNKNLFEPHNWR 420
              E+AGENALE Y    Y+QIL  +R    N        +  FTYLR++  +F+ +N+ 
Sbjct: 379 EGIEVAGENALETYGAKGYNQILLNARPNGVNPNGKPKLRMYGFTYLRLSDTVFQENNFE 438

BLAST of Moc10g28230 vs. TAIR 10
Match: AT4G15210.2 (beta-amylase 5 )

HSP 1 Score: 429.1 bits (1102), Expect = 4.4e-120
Identity = 207/392 (52.81%), Postives = 263/392 (67.09%), Query Frame = 0

Query: 1   MLPLDTVSVGGSLNKARAMNASLMALK-SAGVEGVMVDAWWGLVEKDGPLKYNWEGYAEL 60
           MLPL  V+V         +   L  LK  AGV+GVMVD WWG++E  GP +Y+W  Y  L
Sbjct: 19  MLPLGVVNVENVFADPETLETQLKRLKEEAGVDGVMVDVWWGIIESKGPKQYDWTAYKTL 78

Query: 61  VQMVQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRRNPEYI 120
            Q++ + GLK+Q +MSFHQCGGNVGD  +IP+P WV +    +PD+ YT+R G R+ EY+
Sbjct: 79  FQLIARLGLKIQAIMSFHQCGGNVGDIVTIPIPQWVRDVGDNDPDIYYTNRKGTRDIEYL 138

Query: 121 SLGCDSLPVLKGRTPIQVYADYMRSFRDRFRDYL-GEVIVEIQVGAGPCGELRYPSYPES 180
           S+G D+LP+  GRT +Q+Y+DYM SF++   D +   VIV+I+VG GP GELRYPSYP+S
Sbjct: 139 SIGVDNLPLFAGRTAVQLYSDYMSSFKENMADLIEAGVIVDIEVGLGPAGELRYPSYPQS 198

Query: 181 NGTWRFPGIGEFQCYDKYMRASLEAAAEAAGKREWGSNGPHDSGQYNQFPEDTGFFKRDG 240
            G W FPGIGEFQCYDKY++   + AA  AG  EW  + P D+G+YN  PE+TGFFK+DG
Sbjct: 199 QG-WVFPGIGEFQCYDKYLKKDFKEAAAKAGHPEW--DLPEDAGEYNDKPEETGFFKKDG 258

Query: 241 TWKSEYGKFFLEWYSGKLLQHGDMILAAAKGIFQGTGAKLSAKVAGIHWHYQTQSHAAEL 300
           T+ SE GKFF+ WYS KL+ HGD IL  A  IF G    L+AKV+GIHW Y   SHAAEL
Sbjct: 259 TYVSEKGKFFMTWYSNKLIFHGDQILGEANKIFAGLKVNLAAKVSGIHWLYNHHSHAAEL 318

Query: 301 TAGYYNTRHRDGYSPIAKMLAKHGAVFNFTCMEMKDGQQPSHGNCSPEGLVAQVKMVTKD 360
           TAGYYN   RDGY PIA+ML+KH  + NFTC+EMKD    +    +P+ LV +V      
Sbjct: 319 TAGYYNLFKRDGYRPIARMLSKHYGILNFTCLEMKDTDNTAEALSAPQELVQEVLSKAWK 378

Query: 361 TKTELAGENALERYDGGAYHQILATSRSESGN 391
              E+AGENALE Y    Y+QIL  +R    N
Sbjct: 379 EGIEVAGENALETYGAKGYNQILLNARPNGVN 407

BLAST of Moc10g28230 vs. TAIR 10
Match: AT4G00490.1 (beta-amylase 2 )

HSP 1 Score: 426.0 bits (1094), Expect = 3.8e-119
Identity = 214/427 (50.12%), Postives = 273/427 (63.93%), Query Frame = 0

Query: 1   MLPLDTVSVGGSLNKARAMNASLMALKSAGVEGVMVDAWWGLVEKDGPLKYNWEGYAELV 60
           MLPL  + +   + +   +   L  LKS  V+GVMVD WWG+VE   P  YNW GY +L 
Sbjct: 100 MLPLGVIDMNSEVVEPEELLDQLRTLKSVNVDGVMVDCWWGIVESHTPQVYNWSGYKKLF 159

Query: 61  QMVQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRRNPEYIS 120
           QM+++ GLK+QVVMSFH+CGGNVGD   I +P WV E    NPD+ +TD +GRRN E ++
Sbjct: 160 QMIRELGLKIQVVMSFHECGGNVGDDVHIQIPEWVREIGQSNPDIYFTDSAGRRNTECLT 219

Query: 121 LGCDSLPVLKGRTPIQVYADYMRSFRDRFRDYLGE-VIVEIQVGAGPCGELRYPSYPESN 180
            G D   VL+GRT ++VY DYMRSFR  F ++  E +I EI+VG GPCGELRYPSYP   
Sbjct: 220 WGIDKQRVLRGRTALEVYFDYMRSFRVEFDEFFEEKIIPEIEVGLGPCGELRYPSYPAQF 279

Query: 181 GTWRFPGIGEFQCYDKYMRASLEAAAEAAGKREWGSNGPHDSGQYNQFPEDTGFFKRDGT 240
           G W++PGIGEFQCYDKY+  SL+ AAE  G   WG  GP ++  YN  P  TGFF+  G 
Sbjct: 280 G-WKYPGIGEFQCYDKYLMNSLKEAAEVRGHSFWG-RGPDNTETYNSTPHGTGFFRDGGD 339

Query: 241 WKSEYGKFFLEWYSGKLLQHGDMILAAAKGIFQGTGAKLSAKVAGIHWHYQTQSHAAELT 300
           + S YG+FFL WYS  L+ HGD +LA A   F+GT   ++AK++GIHW Y+T SHAAELT
Sbjct: 340 YDSYYGRFFLNWYSRVLIDHGDRVLAMANLAFEGT--CIAAKLSGIHWWYKTASHAAELT 399

Query: 301 AGYYNTRHRDGYSPIAKMLAKHGAVFNFTCMEMKDGQQPS---HGNCSPEGLVAQVKMVT 360
           AG+YN+ +RDGY PIA M  KH A  NFTC+E++   Q          PEGLV QV    
Sbjct: 400 AGFYNSSNRDGYGPIAAMFKKHDAALNFTCVELRTLDQHEDFPEALADPEGLVWQVLNAA 459

Query: 361 KDTKTELAGENALERYDGGAYHQILATSR---SESGNGLAAFTYLRMNKNLFEPHNWRNL 420
            D    +A ENAL  YD   Y++IL  ++      G  L+ FTYLR+N  L E  N++  
Sbjct: 460 WDASIPVASENALPCYDREGYNKILENAKPLTDPDGRHLSCFTYLRLNPTLMESQNFKEF 519

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022155496.12.6e-271100.00beta-amylase 3, chloroplastic [Momordica charantia][more]
XP_008448759.11.6e-24991.17PREDICTED: beta-amylase 3, chloroplastic [Cucumis melo] >KAA0041034.1 beta-amyla... [more]
XP_004147264.11.1e-24891.19beta-amylase 3, chloroplastic [Cucumis sativus] >KGN55808.1 hypothetical protein... [more]
XP_023007780.11.8e-24890.33beta-amylase 3, chloroplastic-like [Cucurbita maxima][more]
XP_022923359.15.2e-24890.11beta-amylase 3, chloroplastic-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
O235532.0e-22178.02Beta-amylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BAM3 PE=1 SV=3[more]
Q9LIR62.8e-16761.12Beta-amylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BAM1 PE=1 SV=1[more]
Q9AV883.6e-16763.18Beta-amylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=BAMY1 ... [more]
Q10RZ18.1e-16763.93Beta-amylase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=BAMY2 ... [more]
O225858.7e-12952.10Beta-amylase OS=Medicago sativa OX=3879 GN=BMY1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1DRU11.3e-271100.00Beta-amylase OS=Momordica charantia OX=3673 GN=LOC111022628 PE=3 SV=1[more]
A0A5D3CKL17.9e-25091.17Beta-amylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1017G00910 ... [more]
A0A1S3BKF97.9e-25091.17Beta-amylase OS=Cucumis melo OX=3656 GN=LOC103490827 PE=3 SV=1[more]
A0A0A0L2155.1e-24991.19Beta-amylase OS=Cucumis sativus OX=3659 GN=Csa_3G017020 PE=3 SV=1[more]
A0A6J1L3X78.7e-24990.33Beta-amylase OS=Cucurbita maxima OX=3661 GN=LOC111500288 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G17090.11.4e-22278.02chloroplast beta-amylase [more]
AT3G23920.12.0e-16861.12beta-amylase 1 [more]
AT4G15210.18.6e-12450.35beta-amylase 5 [more]
AT4G15210.24.4e-12052.81beta-amylase 5 [more]
AT4G00490.13.8e-11950.12beta-amylase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001554Glycoside hydrolase, family 14PRINTSPR00750BETAAMYLASEcoord: 30..44
score: 72.0
coord: 51..69
score: 47.89
coord: 73..94
score: 72.5
coord: 240..259
score: 58.0
coord: 293..304
score: 85.42
coord: 348..370
score: 42.61
coord: 311..334
score: 54.17
coord: 165..187
score: 55.43
coord: 276..292
score: 46.76
IPR001554Glycoside hydrolase, family 14PFAMPF01373Glyco_hydro_14coord: 1..416
e-value: 6.9E-167
score: 555.5
IPR001554Glycoside hydrolase, family 14PANTHERPTHR31352FAMILY NOT NAMEDcoord: 1..452
IPR001371Glycoside hydrolase, family 14B, plantPRINTSPR00842GLHYDLASE14Bcoord: 314..324
score: 53.85
coord: 155..164
score: 53.08
coord: 396..410
score: 43.08
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 1..453
e-value: 1.0E-211
score: 705.6
NoneNo IPR availablePANTHERPTHR31352:SF1BETA-AMYLASE 3, CHLOROPLASTICcoord: 1..452
IPR018238Glycoside hydrolase, family 14, conserved sitePROSITEPS00506BETA_AMYLASE_1coord: 77..85
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 1..423

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc10g28230.1Moc10g28230.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000272 polysaccharide catabolic process
molecular_function GO:0102229 amylopectin maltohydrolase activity
molecular_function GO:0016161 beta-amylase activity