Moc10g28020 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc10g28020
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionLINE-1 retrotransposable element ORF2 protein
Locationchr10: 20914883 .. 20915380 (-)
RNA-Seq ExpressionMoc10g28020
SyntenyMoc10g28020
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGAATTGTAGTTTTTTCTCTAAGTTCTTGGAGTTCAAAGCGAGAAGAGGGAATCGTATCAAGTTCTGGACAGACAAGTGGATAGGACAAAACACCCTCCAAAGCTCCTTTCCCAACTTGTTTAATATCTCCACAAAAAAGGGAGCTTCTATTGCTGATTGCTGGAATAATTCTGAGAACGATTGGGATCTTGGCTCCAGAAGAGGCCTCTTTGACTGGGAAATAGAAAATTGGATGGGTCTGATCCATGCCATCGACACAGTGGGCCTAGGGGAAGGGGAAGATCGGGTCGGCTGGTCTATTGGCAATGGTGGAAAGTTTTCTACTAGGTCTGCTTGTATGAAGATGGTGGAATGTACTCCCCCTTTGAAATCTTCTCTCATTCAAGCTATTTGGATGTTTGACTCTCCAAGAAAGGTCAAGGTGTTCCTGTGGTCCTTAGCACACAGAAGCTTGAACACTCATGAGAAGCTTCAAAAGAAATGCAGCAATTGA

mRNA sequence

ATGAAGAATTGTAGTTTTTTCTCTAAGTTCTTGGAGTTCAAAGCGAGAAGAGGGAATCGTATCAAGTTCTGGACAGACAAGTGGATAGGACAAAACACCCTCCAAAGCTCCTTTCCCAACTTGTTTAATATCTCCACAAAAAAGGGAGCTTCTATTGCTGATTGCTGGAATAATTCTGAGAACGATTGGGATCTTGGCTCCAGAAGAGGCCTCTTTGACTGGGAAATAGAAAATTGGATGGGTCTGATCCATGCCATCGACACAGTGGGCCTAGGGGAAGGGGAAGATCGGGTCGGCTGGTCTATTGGCAATGGTGGAAAGTTTTCTACTAGGTCTGCTTGTATGAAGATGGTGGAATGTACTCCCCCTTTGAAATCTTCTCTCATTCAAGCTATTTGGATGTTTGACTCTCCAAGAAAGGTCAAGGTGTTCCTGTGGTCCTTAGCACACAGAAGCTTGAACACTCATGAGAAGCTTCAAAAGAAATGCAGCAATTGA

Coding sequence (CDS)

ATGAAGAATTGTAGTTTTTTCTCTAAGTTCTTGGAGTTCAAAGCGAGAAGAGGGAATCGTATCAAGTTCTGGACAGACAAGTGGATAGGACAAAACACCCTCCAAAGCTCCTTTCCCAACTTGTTTAATATCTCCACAAAAAAGGGAGCTTCTATTGCTGATTGCTGGAATAATTCTGAGAACGATTGGGATCTTGGCTCCAGAAGAGGCCTCTTTGACTGGGAAATAGAAAATTGGATGGGTCTGATCCATGCCATCGACACAGTGGGCCTAGGGGAAGGGGAAGATCGGGTCGGCTGGTCTATTGGCAATGGTGGAAAGTTTTCTACTAGGTCTGCTTGTATGAAGATGGTGGAATGTACTCCCCCTTTGAAATCTTCTCTCATTCAAGCTATTTGGATGTTTGACTCTCCAAGAAAGGTCAAGGTGTTCCTGTGGTCCTTAGCACACAGAAGCTTGAACACTCATGAGAAGCTTCAAAAGAAATGCAGCAATTGA

Protein sequence

MKNCSFFSKFLEFKARRGNRIKFWTDKWIGQNTLQSSFPNLFNISTKKGASIADCWNNSENDWDLGSRRGLFDWEIENWMGLIHAIDTVGLGEGEDRVGWSIGNGGKFSTRSACMKMVECTPPLKSSLIQAIWMFDSPRKVKVFLWSLAHRSLNTHEKLQKKCSN
Homology
BLAST of Moc10g28020 vs. NCBI nr
Match: KAA0037436.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 104.8 bits (260), Expect = 7.5e-19
Identity = 52/168 (30.95%), Postives = 91/168 (54.17%), Query Frame = 0

Query: 1   MKNCSFFSKFLEFKARRGNRIKFWTDKWIGQNTLQSSFPNLFNISTKKGASIADCWNNSE 60
           +K   +F     ++ + G  + FW   WI   +L +++P L+ +S  + A+I + W+ + 
Sbjct: 558 IKEIGWFQNHSSWEIKDGENLSFWHSNWIASGSLSNTYPRLYTLSKAQNATIEELWDPNT 617

Query: 61  NDWDLGSRRGLFDWEIENWMGLIHAIDTVGLGEGEDRVGWSIGNGGKFSTRSACMKMV-- 120
           NDW+L  RR L D EIE W  L   +  + L  G++ + W+  + G ++  SA  +++  
Sbjct: 618 NDWNLHPRRPLNDREIELWTNLKGILKILTLNGGKNNMIWTPNDKGTYTAASAKRQLLSE 677

Query: 121 ECTPPL-KSSLIQAIWMFDSPRKVKVFLWSLAHRSLNTHEKLQKKCSN 166
             TP L + +  + +W    P+K K F+WSL H S+NT + LQK+  N
Sbjct: 678 RPTPNLNERNFYETLWKSKLPKKGKFFMWSLFHESINTTDVLQKRLPN 725

BLAST of Moc10g28020 vs. NCBI nr
Match: TYK00226.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 104.8 bits (260), Expect = 7.5e-19
Identity = 52/168 (30.95%), Postives = 91/168 (54.17%), Query Frame = 0

Query: 1   MKNCSFFSKFLEFKARRGNRIKFWTDKWIGQNTLQSSFPNLFNISTKKGASIADCWNNSE 60
           +K   +F     ++ + G  + FW   WI   +L +++P L+ +S  + A+I + W+ + 
Sbjct: 533 IKEIGWFQNHSSWEIKDGENLSFWHSNWIASGSLSNTYPRLYTLSKAQNATIEELWDPNT 592

Query: 61  NDWDLGSRRGLFDWEIENWMGLIHAIDTVGLGEGEDRVGWSIGNGGKFSTRSACMKMV-- 120
           NDW+L  RR L D EIE W  L   +  + L  G++ + W+  + G ++  SA  +++  
Sbjct: 593 NDWNLHPRRPLNDREIELWTNLKGILKILTLNGGKNNMIWTPNDKGTYTAASAKRQLLSE 652

Query: 121 ECTPPL-KSSLIQAIWMFDSPRKVKVFLWSLAHRSLNTHEKLQKKCSN 166
             TP L + +  + +W    P+K K F+WSL H S+NT + LQK+  N
Sbjct: 653 RPTPNLNERNFYETLWKSKLPKKGKFFMWSLFHESINTTDVLQKRLPN 700

BLAST of Moc10g28020 vs. NCBI nr
Match: KAA0044556.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 103.6 bits (257), Expect = 1.7e-18
Identity = 56/167 (33.53%), Postives = 82/167 (49.10%), Query Frame = 0

Query: 3   NC-SFFSKFLEFKARRGNRIKFWTDKWIGQNTLQSSFPNLFNISTKKGASIADCWNNSEN 62
           NC S+F K + +K   G  I FW D W G + L  + P LF +ST K  S+ D WN S  
Sbjct: 644 NCISWFYKNIGWKVNDGEDISFWLDNWNGNSPLSLAVPRLFALSTNKKGSVKDLWNPSLK 703

Query: 63  DWDLGSRRGLFDWEIENWMGLIHAIDTVGLGEGEDRVGWSIGNGGKFSTRSACMKMVECT 122
           DW++   R L D E   W  +  ++ T     G  +  W + +   F T S    + E +
Sbjct: 704 DWNIHVNRPLRDHEKNLWHNIKASLPTPLPDRGPSKPLWKLNSNNIFDTASIKKDLSEAS 763

Query: 123 PP---LKSSLIQAIWMFDSPRKVKVFLWSLAHRSLNTHEKLQKKCSN 166
                   SL + +W  D P+K K F+W+L H  +NT ++LQK+  N
Sbjct: 764 ASPTNFHPSLYKTLWKVDFPKKCKFFIWTLIHGCINTADRLQKRLPN 810

BLAST of Moc10g28020 vs. NCBI nr
Match: MCH99327.1 (ribonuclease H protein [Trifolium medium])

HSP 1 Score: 103.6 bits (257), Expect = 1.7e-18
Identity = 58/165 (35.15%), Postives = 84/165 (50.91%), Query Frame = 0

Query: 5   SFFSKFLEFKARRGNRIKFWTDKWIGQNTLQSSFPNLFNISTKKGASIADCWNNSEND-W 64
           ++FS+ +  K   G +  FW DKW+G  TL+  FP LF+IST+K  S+A  WN   ++ W
Sbjct: 46  NWFSQGVIKKLGNGEQTSFWKDKWVGDTTLKDRFPRLFSISTQKDVSVASLWNRDGSERW 105

Query: 65  DLGSRRGLFDWEIENWMGLIHAIDTVGLGEGEDRVGWSIGNGGKFSTRSA------CMKM 124
           +L  RR LF WE   W  L+  I++V L   ED+  W +   G FS +S        +  
Sbjct: 106 NLEWRRRLFVWESTLWEELVLIINSVTLSCEEDKWEWKLDKNGVFSVKSTYELVSKLLIT 165

Query: 125 VECTPPLKSSLIQAIWMFDSPRKVKVFLWSLAHRSLNTHEKLQKK 163
              T   + S  +AIW   +P KV  F W L H  + T   L ++
Sbjct: 166 TRSTTQEQVSAFKAIWKCPAPSKVLGFSWMLLHDRIPTRVNLFRR 210

BLAST of Moc10g28020 vs. NCBI nr
Match: TYK06777.1 (LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa])

HSP 1 Score: 102.8 bits (255), Expect = 2.8e-18
Identity = 52/165 (31.52%), Postives = 87/165 (52.73%), Query Frame = 0

Query: 2   KNCSFFSKFLEFKARRGNRIKFWTDKWIGQNTLQSSFPNLFNISTKKGASIADCWNNSEN 61
           K   +F + + +K + G    FW   W   + L S +P L+ +ST K +SI D WNN+  
Sbjct: 821 KGLEWFQRHVSWKIKNGRSFSFWHGHWHQNSPLSSHYPRLYALSTNKESSIRDMWNNTLM 880

Query: 62  DWDLGSRRGLFDWEIENWMGLIHAIDTVGLGEGEDRVGWSIGNGGKF---STRSACMKMV 121
           DWDL  RR L +WE+  W  L ++I+      G+D   W++ + G +   S + A  +  
Sbjct: 881 DWDLNPRRQLREWELPLWAELKNSINASFCENGKDSPMWALNSNGLYTVASVKKALQQPE 940

Query: 122 ECTPPLKS-SLIQAIWMFDSPRKVKVFLWSLAHRSLNTHEKLQKK 163
           +    L+S +  + +W    P+K   F+W+L + S+NT E+L K+
Sbjct: 941 QSLLDLQSQNTFKNLWKTSIPKKCIFFIWTLLYDSVNTAEQLMKR 985

BLAST of Moc10g28020 vs. ExPASy TrEMBL
Match: A0A5A7T5L3 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold277G00210 PE=4 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 3.6e-19
Identity = 52/168 (30.95%), Postives = 91/168 (54.17%), Query Frame = 0

Query: 1   MKNCSFFSKFLEFKARRGNRIKFWTDKWIGQNTLQSSFPNLFNISTKKGASIADCWNNSE 60
           +K   +F     ++ + G  + FW   WI   +L +++P L+ +S  + A+I + W+ + 
Sbjct: 558 IKEIGWFQNHSSWEIKDGENLSFWHSNWIASGSLSNTYPRLYTLSKAQNATIEELWDPNT 617

Query: 61  NDWDLGSRRGLFDWEIENWMGLIHAIDTVGLGEGEDRVGWSIGNGGKFSTRSACMKMV-- 120
           NDW+L  RR L D EIE W  L   +  + L  G++ + W+  + G ++  SA  +++  
Sbjct: 618 NDWNLHPRRPLNDREIELWTNLKGILKILTLNGGKNNMIWTPNDKGTYTAASAKRQLLSE 677

Query: 121 ECTPPL-KSSLIQAIWMFDSPRKVKVFLWSLAHRSLNTHEKLQKKCSN 166
             TP L + +  + +W    P+K K F+WSL H S+NT + LQK+  N
Sbjct: 678 RPTPNLNERNFYETLWKSKLPKKGKFFMWSLFHESINTTDVLQKRLPN 725

BLAST of Moc10g28020 vs. ExPASy TrEMBL
Match: A0A5D3BPP1 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1582G00200 PE=4 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 3.6e-19
Identity = 52/168 (30.95%), Postives = 91/168 (54.17%), Query Frame = 0

Query: 1   MKNCSFFSKFLEFKARRGNRIKFWTDKWIGQNTLQSSFPNLFNISTKKGASIADCWNNSE 60
           +K   +F     ++ + G  + FW   WI   +L +++P L+ +S  + A+I + W+ + 
Sbjct: 533 IKEIGWFQNHSSWEIKDGENLSFWHSNWIASGSLSNTYPRLYTLSKAQNATIEELWDPNT 592

Query: 61  NDWDLGSRRGLFDWEIENWMGLIHAIDTVGLGEGEDRVGWSIGNGGKFSTRSACMKMV-- 120
           NDW+L  RR L D EIE W  L   +  + L  G++ + W+  + G ++  SA  +++  
Sbjct: 593 NDWNLHPRRPLNDREIELWTNLKGILKILTLNGGKNNMIWTPNDKGTYTAASAKRQLLSE 652

Query: 121 ECTPPL-KSSLIQAIWMFDSPRKVKVFLWSLAHRSLNTHEKLQKKCSN 166
             TP L + +  + +W    P+K K F+WSL H S+NT + LQK+  N
Sbjct: 653 RPTPNLNERNFYETLWKSKLPKKGKFFMWSLFHESINTTDVLQKRLPN 700

BLAST of Moc10g28020 vs. ExPASy TrEMBL
Match: A0A392NHI5 (Ribonuclease H protein (Fragment) OS=Trifolium medium OX=97028 PE=4 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 8.1e-19
Identity = 58/165 (35.15%), Postives = 84/165 (50.91%), Query Frame = 0

Query: 5   SFFSKFLEFKARRGNRIKFWTDKWIGQNTLQSSFPNLFNISTKKGASIADCWNNSEND-W 64
           ++FS+ +  K   G +  FW DKW+G  TL+  FP LF+IST+K  S+A  WN   ++ W
Sbjct: 46  NWFSQGVIKKLGNGEQTSFWKDKWVGDTTLKDRFPRLFSISTQKDVSVASLWNRDGSERW 105

Query: 65  DLGSRRGLFDWEIENWMGLIHAIDTVGLGEGEDRVGWSIGNGGKFSTRSA------CMKM 124
           +L  RR LF WE   W  L+  I++V L   ED+  W +   G FS +S        +  
Sbjct: 106 NLEWRRRLFVWESTLWEELVLIINSVTLSCEEDKWEWKLDKNGVFSVKSTYELVSKLLIT 165

Query: 125 VECTPPLKSSLIQAIWMFDSPRKVKVFLWSLAHRSLNTHEKLQKK 163
              T   + S  +AIW   +P KV  F W L H  + T   L ++
Sbjct: 166 TRSTTQEQVSAFKAIWKCPAPSKVLGFSWMLLHDRIPTRVNLFRR 210

BLAST of Moc10g28020 vs. ExPASy TrEMBL
Match: A0A5A7TR15 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G003050 PE=4 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 8.1e-19
Identity = 56/167 (33.53%), Postives = 82/167 (49.10%), Query Frame = 0

Query: 3   NC-SFFSKFLEFKARRGNRIKFWTDKWIGQNTLQSSFPNLFNISTKKGASIADCWNNSEN 62
           NC S+F K + +K   G  I FW D W G + L  + P LF +ST K  S+ D WN S  
Sbjct: 644 NCISWFYKNIGWKVNDGEDISFWLDNWNGNSPLSLAVPRLFALSTNKKGSVKDLWNPSLK 703

Query: 63  DWDLGSRRGLFDWEIENWMGLIHAIDTVGLGEGEDRVGWSIGNGGKFSTRSACMKMVECT 122
           DW++   R L D E   W  +  ++ T     G  +  W + +   F T S    + E +
Sbjct: 704 DWNIHVNRPLRDHEKNLWHNIKASLPTPLPDRGPSKPLWKLNSNNIFDTASIKKDLSEAS 763

Query: 123 PP---LKSSLIQAIWMFDSPRKVKVFLWSLAHRSLNTHEKLQKKCSN 166
                   SL + +W  D P+K K F+W+L H  +NT ++LQK+  N
Sbjct: 764 ASPTNFHPSLYKTLWKVDFPKKCKFFIWTLIHGCINTADRLQKRLPN 810

BLAST of Moc10g28020 vs. ExPASy TrEMBL
Match: A0A5D3C4J1 (LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00790 PE=4 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 1.4e-18
Identity = 52/165 (31.52%), Postives = 87/165 (52.73%), Query Frame = 0

Query: 2   KNCSFFSKFLEFKARRGNRIKFWTDKWIGQNTLQSSFPNLFNISTKKGASIADCWNNSEN 61
           K   +F + + +K + G    FW   W   + L S +P L+ +ST K +SI D WNN+  
Sbjct: 821 KGLEWFQRHVSWKIKNGRSFSFWHGHWHQNSPLSSHYPRLYALSTNKESSIRDMWNNTLM 880

Query: 62  DWDLGSRRGLFDWEIENWMGLIHAIDTVGLGEGEDRVGWSIGNGGKF---STRSACMKMV 121
           DWDL  RR L +WE+  W  L ++I+      G+D   W++ + G +   S + A  +  
Sbjct: 881 DWDLNPRRQLREWELPLWAELKNSINASFCENGKDSPMWALNSNGLYTVASVKKALQQPE 940

Query: 122 ECTPPLKS-SLIQAIWMFDSPRKVKVFLWSLAHRSLNTHEKLQKK 163
           +    L+S +  + +W    P+K   F+W+L + S+NT E+L K+
Sbjct: 941 QSLLDLQSQNTFKNLWKTSIPKKCIFFIWTLLYDSVNTAEQLMKR 985

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0037436.17.5e-1930.95LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa][more]
TYK00226.17.5e-1930.95LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa][more]
KAA0044556.11.7e-1833.53LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa][more]
MCH99327.11.7e-1835.15ribonuclease H protein [Trifolium medium][more]
TYK06777.12.8e-1831.52LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5A7T5L33.6e-1930.95LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=119... [more]
A0A5D3BPP13.6e-1930.95LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=119... [more]
A0A392NHI58.1e-1935.15Ribonuclease H protein (Fragment) OS=Trifolium medium OX=97028 PE=4 SV=1[more]
A0A5A7TR158.1e-1933.53LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=119... [more]
A0A5D3C4J11.4e-1831.52LINE-1 retrotransposable element ORF2 protein OS=Cucumis melo var. makuwa OX=119... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR36617:SF3SUBFAMILY NOT NAMEDcoord: 18..163
NoneNo IPR availablePANTHERPTHR36617PROTEIN, PUTATIVE-RELATEDcoord: 18..163

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc10g28020.1Moc10g28020.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042254 ribosome biogenesis
biological_process GO:0016070 RNA metabolic process
cellular_component GO:0043229 intracellular organelle
molecular_function GO:1901363 heterocyclic compound binding
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0097159 organic cyclic compound binding