Moc10g25860 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc10g25860
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Acetolactate synthase
Location	chr10: 18768287 .. 18770347 (-)
RNA-Seq Expression	Moc10g25860
Synteny	Moc10g25860

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTTCATCTGGCCTCCTTCTCCATGCCATCAATGGCAGCTATGTCCTTCTGCACCTCCATCTCCAAATACTCTTCTTCCTCTACCTCTTTCAAGCCTTCGAAACCCATCTCCCCATTCTTTCAATCATTCTCTATTCTTACCCAAAATCACACCTTTTTCTGCCGTCCTATTCGCATCTGTAGCTCTCTGTCTCCATCCTCCGTCCCTGAAATTTTCATCGTCTCTCGATTTGCCGCCGATGAGCCGAGAAAAGGCGCTGATATCCTCATCGAGGCTCTCGAGCGCCAAGGCGTCACTAGTGTCTTCGCTTACCCCGGCGGCGCGTCCATGGAGATCCACCAGGCCCTCACACGCTCCGCGATCATCCGCGACATCCTCCCCCGCCATGAGCAGGGCGGCGTCTTTGCTGCCGAGGGATACGCACGCTCCTCCGGCCTTCCGGGCGTCTGCATTGCAACTTCGGGGCCTGGCGCCACCAACTTGGTCACCGGCCTCGCCGATGCCCTCCTCGATAGCGTTCCACTAGTGGCCATCACCGGCCAAGTGCCGAGAGGAATGATGGGTAACAATGCGTTTCAGGACATTCCCATCGTTGAAGTAACTCGCTCCATCACAAAGCACAATTATCTGGTTCTTGACGTAGATGACATTCCAAGGATCGTGAGCGAGGCATTTTTCCTCGCAACTTCTGGTCGGCCTGGTCCTGTTCTTATTGATATCCCCAAAGATATTCAGCAACAGCTCGCGATCCCCAACTGGAATCAGCCCATCAAATTATCGGCTTACTTGTCTACCTTACCAAACCCTCCGAACGATGCCCCTTTGGATCAAATTTTGCGACTGATCTCTGAATCCAAGAAGCCAGTTCTGTACGTGGGTGGCGGGTGTTTGAATTCACCGGACGAGTTGCGGCGGTTTGTCGGCCTCACTGGGATTCCGGTGGCGAGTACTCTGATGGGTCTTGGGGCGTATCCATGCAACCACGAGCTGTCCTTACAAATGCTGGGAATGCATGGGACAGTTTACGCTAATTACGCTGTGGATAAATCTGACCTCTTGCTTGCATTTGGGGTGAGATTCGACGAGCGTGTGACGGGGAAGCTTGAGGCATTTGCGAGCCGGGCTAAAATCGTTCATATTGATATCGACTCGTACGAGATCGGGAGGAACAAGCAGCCCCACGTATGGGTTTGTGGCGACGTGAAGTTGGCCATGCAGAGAATGAACCGTTTAATGGAAGAGAACGCAGAGAAGCTTCTTCATTGTGATTTCTCTGCGTGGAGAGAGGAACTGGATGGGCAGAAATCAAAGTACCCACTGAGTTACAAGACATTCGAGGAAGCCATTCCTCCCCAGTATGCCATCCAAGTCCTTGATGAGCTAACAAATGGCAGGGCGATCATAAGTACAGGAGTATGATATATGAATTAAATTTACCAAATTGATCGGTTCAAGCTTTTAAGTTGAGTGTTGAGTGGTGTTTTATTTCATCAGGTGGGGCAGCACCAGATGTGGGTGGCTCAGTTCTACAAGTTCAAGAAGCCACGCCAGTGGCTAACCTCGGGCGGGCTAGGAGCGATGGGGTTCGGACTGCCGGCAGCCATGGGCGCAGTAGTAGCAAATCCCGAGGCGGTGGTGGTAGACGTTGACGGGGACGGGAGCTTCATGATGAACATCCAAGAACTAGCAGCCATCAGTGTGGAGAAACTTCCCGTCAAGATCCTGCTGTTGAACAACCAGTATTTGGGTATGGTTTTGCAGTGGGAGGATCAGTTCAACAAATTAGGCAACAGGGCTCAGACGTACCTTGGAGATCCATCGAAGGGATCAGAGATATTCCCAAACATGTTGAAGTTTGCAGAGGCCTGTGGGATCCCGGCGGCGCGGGTGACGAAGAAGTGGGAGGTAAAAACAGCGATAAAGAAGATGCTGGAGACTGAGGGGCCTTACTTGTTGGATGTCATCATACCGCATCAAGAATATGTGCTGCCTCTCATTCCCAGCGGTGGCACTTTCAAAGATGTGATCACAGAGGGAGATGGAAGATCCCAATCATAA

mRNA sequence

ATGCTTCATCTGGCCTCCTTCTCCATGCCATCAATGGCAGCTATGTCCTTCTGCACCTCCATCTCCAAATACTCTTCTTCCTCTACCTCTTTCAAGCCTTCGAAACCCATCTCCCCATTCTTTCAATCATTCTCTATTCTTACCCAAAATCACACCTTTTTCTGCCGTCCTATTCGCATCTGTAGCTCTCTGTCTCCATCCTCCGTCCCTGAAATTTTCATCGTCTCTCGATTTGCCGCCGATGAGCCGAGAAAAGGCGCTGATATCCTCATCGAGGCTCTCGAGCGCCAAGGCGTCACTAGTGTCTTCGCTTACCCCGGCGGCGCGTCCATGGAGATCCACCAGGCCCTCACACGCTCCGCGATCATCCGCGACATCCTCCCCCGCCATGAGCAGGGCGGCGTCTTTGCTGCCGAGGGATACGCACGCTCCTCCGGCCTTCCGGGCGTCTGCATTGCAACTTCGGGGCCTGGCGCCACCAACTTGGTCACCGGCCTCGCCGATGCCCTCCTCGATAGCGTTCCACTAGTGGCCATCACCGGCCAAGTGCCGAGAGGAATGATGGGTAACAATGCGTTTCAGGACATTCCCATCGTTGAAGTAACTCGCTCCATCACAAAGCACAATTATCTGGTTCTTGACGTAGATGACATTCCAAGGATCGTGAGCGAGGCATTTTTCCTCGCAACTTCTGGTCGGCCTGGTCCTGTTCTTATTGATATCCCCAAAGATATTCAGCAACAGCTCGCGATCCCCAACTGGAATCAGCCCATCAAATTATCGGCTTACTTGTCTACCTTACCAAACCCTCCGAACGATGCCCCTTTGGATCAAATTTTGCGACTGATCTCTGAATCCAAGAAGCCAGTTCTGTACGTGGGTGGCGGGTGTTTGAATTCACCGGACGAGTTGCGGCGGTTTGTCGGCCTCACTGGGATTCCGGTGGCGAGTACTCTGATGGGTCTTGGGGCGTATCCATGCAACCACGAGCTGTCCTTACAAATGCTGGGAATGCATGGGACAGTTTACGCTAATTACGCTGTGGATAAATCTGACCTCTTGCTTGCATTTGGGGTGAGATTCGACGAGCGTGTGACGGGGAAGCTTGAGGCATTTGCGAGCCGGGCTAAAATCGTTCATATTGATATCGACTCGTACGAGATCGGGAGGAACAAGCAGCCCCACGTATGGGTTTGTGGCGACGTGAAGTTGGCCATGCAGAGAATGAACCGTTTAATGGAAGAGAACGCAGAGAAGCTTCTTCATTGTGATTTCTCTGCGTGGAGAGAGGAACTGGATGGGCAGAAATCAAAGTACCCACTGAGTTACAAGACATTCGAGGAAGCCATTCCTCCCCAGTATGCCATCCAAGTCCTTGATGAGCTAACAAATGGCAGGGCGATCATAAGTACAGGAGTGGGGCAGCACCAGATGTGGGTGGCTCAGTTCTACAAGTTCAAGAAGCCACGCCAGTGGCTAACCTCGGGCGGGCTAGGAGCGATGGGGTTCGGACTGCCGGCAGCCATGGGCGCAGTAGTAGCAAATCCCGAGGCGGTGGTGGTAGACGTTGACGGGGACGGGAGCTTCATGATGAACATCCAAGAACTAGCAGCCATCAGTGTGGAGAAACTTCCCGTCAAGATCCTGCTGTTGAACAACCAGTATTTGGGTATGGTTTTGCAGTGGGAGGATCAGTTCAACAAATTAGGCAACAGGGCTCAGACGTACCTTGGAGATCCATCGAAGGGATCAGAGATATTCCCAAACATGTTGAAGTTTGCAGAGGCCTGTGGGATCCCGGCGGCGCGGGTGACGAAGAAGTGGGAGGTAAAAACAGCGATAAAGAAGATGCTGGAGACTGAGGGGCCTTACTTGTTGGATGTCATCATACCGCATCAAGAATATGTGCTGCCTCTCATTCCCAGCGGTGGCACTTTCAAAGATGTGATCACAGAGGGAGATGGAAGATCCCAATCATAA

Coding sequence (CDS)

ATGCTTCATCTGGCCTCCTTCTCCATGCCATCAATGGCAGCTATGTCCTTCTGCACCTCCATCTCCAAATACTCTTCTTCCTCTACCTCTTTCAAGCCTTCGAAACCCATCTCCCCATTCTTTCAATCATTCTCTATTCTTACCCAAAATCACACCTTTTTCTGCCGTCCTATTCGCATCTGTAGCTCTCTGTCTCCATCCTCCGTCCCTGAAATTTTCATCGTCTCTCGATTTGCCGCCGATGAGCCGAGAAAAGGCGCTGATATCCTCATCGAGGCTCTCGAGCGCCAAGGCGTCACTAGTGTCTTCGCTTACCCCGGCGGCGCGTCCATGGAGATCCACCAGGCCCTCACACGCTCCGCGATCATCCGCGACATCCTCCCCCGCCATGAGCAGGGCGGCGTCTTTGCTGCCGAGGGATACGCACGCTCCTCCGGCCTTCCGGGCGTCTGCATTGCAACTTCGGGGCCTGGCGCCACCAACTTGGTCACCGGCCTCGCCGATGCCCTCCTCGATAGCGTTCCACTAGTGGCCATCACCGGCCAAGTGCCGAGAGGAATGATGGGTAACAATGCGTTTCAGGACATTCCCATCGTTGAAGTAACTCGCTCCATCACAAAGCACAATTATCTGGTTCTTGACGTAGATGACATTCCAAGGATCGTGAGCGAGGCATTTTTCCTCGCAACTTCTGGTCGGCCTGGTCCTGTTCTTATTGATATCCCCAAAGATATTCAGCAACAGCTCGCGATCCCCAACTGGAATCAGCCCATCAAATTATCGGCTTACTTGTCTACCTTACCAAACCCTCCGAACGATGCCCCTTTGGATCAAATTTTGCGACTGATCTCTGAATCCAAGAAGCCAGTTCTGTACGTGGGTGGCGGGTGTTTGAATTCACCGGACGAGTTGCGGCGGTTTGTCGGCCTCACTGGGATTCCGGTGGCGAGTACTCTGATGGGTCTTGGGGCGTATCCATGCAACCACGAGCTGTCCTTACAAATGCTGGGAATGCATGGGACAGTTTACGCTAATTACGCTGTGGATAAATCTGACCTCTTGCTTGCATTTGGGGTGAGATTCGACGAGCGTGTGACGGGGAAGCTTGAGGCATTTGCGAGCCGGGCTAAAATCGTTCATATTGATATCGACTCGTACGAGATCGGGAGGAACAAGCAGCCCCACGTATGGGTTTGTGGCGACGTGAAGTTGGCCATGCAGAGAATGAACCGTTTAATGGAAGAGAACGCAGAGAAGCTTCTTCATTGTGATTTCTCTGCGTGGAGAGAGGAACTGGATGGGCAGAAATCAAAGTACCCACTGAGTTACAAGACATTCGAGGAAGCCATTCCTCCCCAGTATGCCATCCAAGTCCTTGATGAGCTAACAAATGGCAGGGCGATCATAAGTACAGGAGTGGGGCAGCACCAGATGTGGGTGGCTCAGTTCTACAAGTTCAAGAAGCCACGCCAGTGGCTAACCTCGGGCGGGCTAGGAGCGATGGGGTTCGGACTGCCGGCAGCCATGGGCGCAGTAGTAGCAAATCCCGAGGCGGTGGTGGTAGACGTTGACGGGGACGGGAGCTTCATGATGAACATCCAAGAACTAGCAGCCATCAGTGTGGAGAAACTTCCCGTCAAGATCCTGCTGTTGAACAACCAGTATTTGGGTATGGTTTTGCAGTGGGAGGATCAGTTCAACAAATTAGGCAACAGGGCTCAGACGTACCTTGGAGATCCATCGAAGGGATCAGAGATATTCCCAAACATGTTGAAGTTTGCAGAGGCCTGTGGGATCCCGGCGGCGCGGGTGACGAAGAAGTGGGAGGTAAAAACAGCGATAAAGAAGATGCTGGAGACTGAGGGGCCTTACTTGTTGGATGTCATCATACCGCATCAAGAATATGTGCTGCCTCTCATTCCCAGCGGTGGCACTTTCAAAGATGTGATCACAGAGGGAGATGGAAGATCCCAATCATAA

Protein sequence

MLHLASFSMPSMAAMSFCTSISKYSSSSTSFKPSKPISPFFQSFSILTQNHTFFCRPIRICSSLSPSSVPEIFIVSRFAADEPRKGADILIEALERQGVTSVFAYPGGASMEIHQALTRSAIIRDILPRHEQGGVFAAEGYARSSGLPGVCIATSGPGATNLVTGLADALLDSVPLVAITGQVPRGMMGNNAFQDIPIVEVTRSITKHNYLVLDVDDIPRIVSEAFFLATSGRPGPVLIDIPKDIQQQLAIPNWNQPIKLSAYLSTLPNPPNDAPLDQILRLISESKKPVLYVGGGCLNSPDELRRFVGLTGIPVASTLMGLGAYPCNHELSLQMLGMHGTVYANYAVDKSDLLLAFGVRFDERVTGKLEAFASRAKIVHIDIDSYEIGRNKQPHVWVCGDVKLAMQRMNRLMEENAEKLLHCDFSAWREELDGQKSKYPLSYKTFEEAIPPQYAIQVLDELTNGRAIISTGVGQHQMWVAQFYKFKKPRQWLTSGGLGAMGFGLPAAMGAVVANPEAVVVDVDGDGSFMMNIQELAAISVEKLPVKILLLNNQYLGMVLQWEDQFNKLGNRAQTYLGDPSKGSEIFPNMLKFAEACGIPAARVTKKWEVKTAIKKMLETEGPYLLDVIIPHQEYVLPLIPSGGTFKDVITEGDGRSQS

Homology

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BLAST of Moc10g25860 vs. NCBI nr
Match: XP_022143464.1 (acetolactate synthase 3, chloroplastic-like [Momordica charantia])

HSP 1 Score: 1316.6 bits (3406), Expect = 0.0e+00
Identity = 659/659 (100.00%), Postives = 659/659 (100.00%), Query Frame = 0


Query: 1   MLHLASFSMPSMAAMSFCTSISKYSSSSTSFKPSKPISPFFQSFSILTQNHTFFCRPIRI 60
           MLHLASFSMPSMAAMSFCTSISKYSSSSTSFKPSKPISPFFQSFSILTQNHTFFCRPIRI
Sbjct: 1   MLHLASFSMPSMAAMSFCTSISKYSSSSTSFKPSKPISPFFQSFSILTQNHTFFCRPIRI 60

Query: 61  CSSLSPSSVPEIFIVSRFAADEPRKGADILIEALERQGVTSVFAYPGGASMEIHQALTRS 120
           CSSLSPSSVPEIFIVSRFAADEPRKGADILIEALERQGVTSVFAYPGGASMEIHQALTRS
Sbjct: 61  CSSLSPSSVPEIFIVSRFAADEPRKGADILIEALERQGVTSVFAYPGGASMEIHQALTRS 120

Query: 121 AIIRDILPRHEQGGVFAAEGYARSSGLPGVCIATSGPGATNLVTGLADALLDSVPLVAIT 180
           AIIRDILPRHEQGGVFAAEGYARSSGLPGVCIATSGPGATNLVTGLADALLDSVPLVAIT
Sbjct: 121 AIIRDILPRHEQGGVFAAEGYARSSGLPGVCIATSGPGATNLVTGLADALLDSVPLVAIT 180

Query: 181 GQVPRGMMGNNAFQDIPIVEVTRSITKHNYLVLDVDDIPRIVSEAFFLATSGRPGPVLID 240
           GQVPRGMMGNNAFQDIPIVEVTRSITKHNYLVLDVDDIPRIVSEAFFLATSGRPGPVLID
Sbjct: 181 GQVPRGMMGNNAFQDIPIVEVTRSITKHNYLVLDVDDIPRIVSEAFFLATSGRPGPVLID 240

Query: 241 IPKDIQQQLAIPNWNQPIKLSAYLSTLPNPPNDAPLDQILRLISESKKPVLYVGGGCLNS 300
           IPKDIQQQLAIPNWNQPIKLSAYLSTLPNPPNDAPLDQILRLISESKKPVLYVGGGCLNS
Sbjct: 241 IPKDIQQQLAIPNWNQPIKLSAYLSTLPNPPNDAPLDQILRLISESKKPVLYVGGGCLNS 300

Query: 301 PDELRRFVGLTGIPVASTLMGLGAYPCNHELSLQMLGMHGTVYANYAVDKSDLLLAFGVR 360
           PDELRRFVGLTGIPVASTLMGLGAYPCNHELSLQMLGMHGTVYANYAVDKSDLLLAFGVR
Sbjct: 301 PDELRRFVGLTGIPVASTLMGLGAYPCNHELSLQMLGMHGTVYANYAVDKSDLLLAFGVR 360

Query: 361 FDERVTGKLEAFASRAKIVHIDIDSYEIGRNKQPHVWVCGDVKLAMQRMNRLMEENAEKL 420
           FDERVTGKLEAFASRAKIVHIDIDSYEIGRNKQPHVWVCGDVKLAMQRMNRLMEENAEKL
Sbjct: 361 FDERVTGKLEAFASRAKIVHIDIDSYEIGRNKQPHVWVCGDVKLAMQRMNRLMEENAEKL 420

Query: 421 LHCDFSAWREELDGQKSKYPLSYKTFEEAIPPQYAIQVLDELTNGRAIISTGVGQHQMWV 480
           LHCDFSAWREELDGQKSKYPLSYKTFEEAIPPQYAIQVLDELTNGRAIISTGVGQHQMWV
Sbjct: 421 LHCDFSAWREELDGQKSKYPLSYKTFEEAIPPQYAIQVLDELTNGRAIISTGVGQHQMWV 480

Query: 481 AQFYKFKKPRQWLTSGGLGAMGFGLPAAMGAVVANPEAVVVDVDGDGSFMMNIQELAAIS 540
           AQFYKFKKPRQWLTSGGLGAMGFGLPAAMGAVVANPEAVVVDVDGDGSFMMNIQELAAIS
Sbjct: 481 AQFYKFKKPRQWLTSGGLGAMGFGLPAAMGAVVANPEAVVVDVDGDGSFMMNIQELAAIS 540

Query: 541 VEKLPVKILLLNNQYLGMVLQWEDQFNKLGNRAQTYLGDPSKGSEIFPNMLKFAEACGIP 600
           VEKLPVKILLLNNQYLGMVLQWEDQFNKLGNRAQTYLGDPSKGSEIFPNMLKFAEACGIP
Sbjct: 541 VEKLPVKILLLNNQYLGMVLQWEDQFNKLGNRAQTYLGDPSKGSEIFPNMLKFAEACGIP 600

Query: 601 AARVTKKWEVKTAIKKMLETEGPYLLDVIIPHQEYVLPLIPSGGTFKDVITEGDGRSQS 660
           AARVTKKWEVKTAIKKMLETEGPYLLDVIIPHQEYVLPLIPSGGTFKDVITEGDGRSQS
Sbjct: 601 AARVTKKWEVKTAIKKMLETEGPYLLDVIIPHQEYVLPLIPSGGTFKDVITEGDGRSQS 659

[Close]

BLAST of Moc10g25860 vs. NCBI nr
Match: XP_008464426.1 (PREDICTED: acetolactate synthase 3, chloroplastic [Cucumis melo] >KAA0057770.1 acetolactate synthase 3 [Cucumis melo var. makuwa] >TYJ98451.1 acetolactate synthase 3 [Cucumis melo var. makuwa])

HSP 1 Score: 1037.7 bits (2682), Expect = 4.2e-299
Identity = 538/667 (80.66%), Postives = 588/667 (88.16%), Query Frame = 0


Query: 12  MAAMSFCTS--ISKYSSSSTSFKPSKPISPFFQSFSILTQNHTFFCRPIRICS-SLS-PS 71
           M++M+  TS   SK  S S+SF  +KP S F  SFS L  N T F RP+RICS SLS PS
Sbjct: 1   MSSMAAATSPCASKSFSKSSSFNLTKPFSRFDLSFSSLPHNPT-FRRPLRICSTSLSNPS 60

Query: 72  SVP------------------EIFIVSRFAADEPRKGADILIEALERQGVTSVFAYPGGA 131
             P                  +IF VSRFAADEPRKGADIL+EALERQGVT+VFAYPGGA
Sbjct: 61  PKPSSTAASAAAVETSSIASSDIF-VSRFAADEPRKGADILVEALERQGVTNVFAYPGGA 120

Query: 132 SMEIHQALTRSAIIRDILPRHEQGGVFAAEGYARSSGLPGVCIATSGPGATNLVTGLADA 191
           SMEIHQALTRS+IIR++LPRHEQGGVFAAEGYARSSGLPGVCIATSGPGATNLV+GLADA
Sbjct: 121 SMEIHQALTRSSIIRNVLPRHEQGGVFAAEGYARSSGLPGVCIATSGPGATNLVSGLADA 180

Query: 192 LLDSVPLVAITGQVPRGMMGNNAFQDIPIVEVTRSITKHNYLVLDVDDIPRIVSEAFFLA 251
           LLDSVPLVAITGQVPR M+G +AFQ+ PIVEVTRSITKHNYLVLDVDDIPRIVSEAFFLA
Sbjct: 181 LLDSVPLVAITGQVPRRMIGTDAFQETPIVEVTRSITKHNYLVLDVDDIPRIVSEAFFLA 240

Query: 252 TSGRPGPVLIDIPKDIQQQLAIPNWNQPIKLSAYLSTLPNPPNDAPLDQILRLISESKKP 311
            SGRPGPVLIDIPKD+QQQLA+PNWNQP+KL  YLS LP PP D+ L+QILRLISESKKP
Sbjct: 241 ISGRPGPVLIDIPKDVQQQLAVPNWNQPMKLPGYLSRLPKPPIDSHLEQILRLISESKKP 300

Query: 312 VLYVGGGCLNSPDELRRFVGLTGIPVASTLMGLGAYPCNHELSLQMLGMHGTVYANYAVD 371
           VLYVGGGCLNS +ELRRFV  TGIPVASTLMGLGAYPC  +LSLQMLGMHGTVYANYAVD
Sbjct: 301 VLYVGGGCLNSSEELRRFVKFTGIPVASTLMGLGAYPCADDLSLQMLGMHGTVYANYAVD 360

Query: 372 KSDLLLAFGVRFDERVTGKLEAFASRAKIVHIDIDSYEIGRNKQPHVWVCGDVKLAMQRM 431
           KSDLLLAFGVRFD+RVTGKLEAFASRAKIVHIDIDS EIG+NKQPHV VCGDVKLA+Q M
Sbjct: 361 KSDLLLAFGVRFDDRVTGKLEAFASRAKIVHIDIDSAEIGKNKQPHVSVCGDVKLALQGM 420

Query: 432 NRLMEENAEKLLHCDFSAWREELDGQKSKYPLSYKTFEEAIPPQYAIQVLDELTNGRAII 491
           NRL+E+   ++LH DFSAWREEL+ QKSKYPL++KTF+EAIPPQYAIQ+LDELTNG AII
Sbjct: 421 NRLLEK--AQMLHFDFSAWREELNEQKSKYPLTFKTFDEAIPPQYAIQLLDELTNGEAII 480

Query: 492 STGVGQHQMWVAQFYKFKKPRQWLTSGGLGAMGFGLPAAMGAVVANPEAVVVDVDGDGSF 551
           STGVGQHQMW AQFYK+KKPRQWLTSGGLGAMGFGLPAAMGAVVANP AVVVD+DGDGSF
Sbjct: 481 STGVGQHQMWAAQFYKYKKPRQWLTSGGLGAMGFGLPAAMGAVVANPGAVVVDIDGDGSF 540

Query: 552 MMNIQELAAISVEKLPVKILLLNNQYLGMVLQWEDQFNKLGNRAQTYLGDPSKGSEIFPN 611
           +MN+QELA ISVEKLPVKILLLNNQ+LGMV+QWED+F K  NRA TYLG+PS  SEIFPN
Sbjct: 541 IMNVQELATISVEKLPVKILLLNNQHLGMVVQWEDRFYK-ANRAHTYLGNPSNESEIFPN 600

Query: 612 MLKFAEACGIPAARVTKKWEVKTAIKKMLETEGPYLLDVIIPHQEYVLPLIPSGGTFKDV 657
           MLKFAEACGIPAARVTK+ E++ A+KKMLETEGPYLLDVI+PHQE+VLP+IPSGG FKDV
Sbjct: 601 MLKFAEACGIPAARVTKRAELRAAMKKMLETEGPYLLDVIVPHQEHVLPMIPSGGAFKDV 660

[Close]

BLAST of Moc10g25860 vs. NCBI nr
Match: XP_038878927.1 (acetolactate synthase 3, chloroplastic [Benincasa hispida])

HSP 1 Score: 1036.6 bits (2679), Expect = 9.5e-299
Identity = 531/671 (79.14%), Postives = 588/671 (87.63%), Query Frame = 0


Query: 9   MPSMAAMSFCTSISKYSSSSTSFKPSKPISPFFQSFSILTQNHTFFCRPIRICSS----- 68
           M SMAA +   +   +S SS+SF   KP S F  S S L  N T F RP+RIC+S     
Sbjct: 1   MSSMAAATSPCASKSFSKSSSSFTLPKPFSRFDLSVSPLPHNPT-FRRPLRICTSSLSNP 60

Query: 69  -----------------LSPSSVPEIFIVSRFAADEPRKGADILIEALERQGVTSVFAYP 128
                             SP + P+IF VSRFA+DEPRKGADIL+EALERQGVT+VFAYP
Sbjct: 61  SPSPKPSSTAASAAAVETSPIATPDIF-VSRFASDEPRKGADILVEALERQGVTNVFAYP 120

Query: 129 GGASMEIHQALTRSAIIRDILPRHEQGGVFAAEGYARSSGLPGVCIATSGPGATNLVTGL 188
           GGASMEIHQALTRS+IIR++LPRHEQGGVFAAEGYARSSGLPGVCIATSGPGATNLV+GL
Sbjct: 121 GGASMEIHQALTRSSIIRNVLPRHEQGGVFAAEGYARSSGLPGVCIATSGPGATNLVSGL 180

Query: 189 ADALLDSVPLVAITGQVPRGMMGNNAFQDIPIVEVTRSITKHNYLVLDVDDIPRIVSEAF 248
           ADALLDSVPLVAITGQVPR M+G +AFQ+ PIVEVTRSITKHNYLVLDVDDIPRIVSEAF
Sbjct: 181 ADALLDSVPLVAITGQVPRRMIGTDAFQETPIVEVTRSITKHNYLVLDVDDIPRIVSEAF 240

Query: 249 FLATSGRPGPVLIDIPKDIQQQLAIPNWNQPIKLSAYLSTLPNPPNDAPLDQILRLISES 308
           FLA SGRPGPVLIDIPKD+QQQLA+PNWNQP+KL  YLS LP PP D+ L+QILRLISES
Sbjct: 241 FLAISGRPGPVLIDIPKDVQQQLAVPNWNQPMKLPGYLSRLPKPPIDSHLEQILRLISES 300

Query: 309 KKPVLYVGGGCLNSPDELRRFVGLTGIPVASTLMGLGAYPCNHELSLQMLGMHGTVYANY 368
           KKPVLYVGGGCLNS +ELRRFV LTGIPVASTLMGLGAYPC+ +LSLQMLGMHGTVYANY
Sbjct: 301 KKPVLYVGGGCLNSSEELRRFVKLTGIPVASTLMGLGAYPCSDDLSLQMLGMHGTVYANY 360

Query: 369 AVDKSDLLLAFGVRFDERVTGKLEAFASRAKIVHIDIDSYEIGRNKQPHVWVCGDVKLAM 428
           AVDKSDLLLAFGVRFD+RVTGKLEAFASRAKIVHIDIDS EIG+NKQPHV VCGDVKLA+
Sbjct: 361 AVDKSDLLLAFGVRFDDRVTGKLEAFASRAKIVHIDIDSAEIGKNKQPHVSVCGDVKLAL 420

Query: 429 QRMNRLMEENAEKLLHCDFSAWREELDGQKSKYPLSYKTFEEAIPPQYAIQVLDELTNGR 488
           Q MNRL+++ AEK +H DFS WREEL+ QKSKYPL++KTF+EAIPPQYAIQ+LDELTNG 
Sbjct: 421 QGMNRLLQK-AEK-VHFDFSPWREELNEQKSKYPLTFKTFDEAIPPQYAIQLLDELTNGE 480

Query: 489 AIISTGVGQHQMWVAQFYKFKKPRQWLTSGGLGAMGFGLPAAMGAVVANPEAVVVDVDGD 548
           AI+STGVGQHQMW AQFYK+KKPRQWLTSGGLGAMGFGLPAAMGA VANP+AVVVD+DGD
Sbjct: 481 AIVSTGVGQHQMWAAQFYKYKKPRQWLTSGGLGAMGFGLPAAMGAAVANPDAVVVDIDGD 540

Query: 549 GSFMMNIQELAAISVEKLPVKILLLNNQYLGMVLQWEDQFNKLGNRAQTYLGDPSKGSEI 608
           GSF+MN+QELA ISVEKLPVKILLLNNQ+LGMV+QWED+F K  NRA TYLG+PS  SEI
Sbjct: 541 GSFIMNVQELATISVEKLPVKILLLNNQHLGMVVQWEDRFYK-ANRAHTYLGNPSNESEI 600

Query: 609 FPNMLKFAEACGIPAARVTKKWEVKTAIKKMLETEGPYLLDVIIPHQEYVLPLIPSGGTF 658
           FPNMLKFAEACGIPAARVTK+ E++ A++KML+TEGPYLLDVI+PHQE+VLP+IPSGG F
Sbjct: 601 FPNMLKFAEACGIPAARVTKRAELRAAMEKMLKTEGPYLLDVIVPHQEHVLPMIPSGGAF 660

[Close]

BLAST of Moc10g25860 vs. NCBI nr
Match: XP_004138045.1 (acetolactate synthase 3, chloroplastic [Cucumis sativus] >KGN63481.1 hypothetical protein Csa_013445 [Cucumis sativus])

HSP 1 Score: 1035.0 bits (2675), Expect = 2.7e-298
Identity = 537/668 (80.39%), Postives = 585/668 (87.57%), Query Frame = 0


Query: 9   MPSMAAMSFCTSISKYSSSSTSFKPSKPISPFFQSFSILTQNHTFFCRPIRICS-SLS-P 68
           M SMAA +     SK  S S SF  +KP S F  SFS L  N T F R +RICS SLS P
Sbjct: 1   MSSMAAAA-SPCASKSFSKSPSFNLTKPFSRFDLSFSSLPHNPT-FRRSLRICSTSLSNP 60

Query: 69  SSVP------------------EIFIVSRFAADEPRKGADILIEALERQGVTSVFAYPGG 128
           S  P                  EIF  SRFAADEPRKGADIL+EALERQGVT+VFAYPGG
Sbjct: 61  SPKPSSTAASAATVETSSITSSEIF-ASRFAADEPRKGADILVEALERQGVTNVFAYPGG 120

Query: 129 ASMEIHQALTRSAIIRDILPRHEQGGVFAAEGYARSSGLPGVCIATSGPGATNLVTGLAD 188
           ASMEIHQALTRS+IIR++LPRHEQGGVFAAEGYARSSGLPGVCIATSGPGATNLV+GLAD
Sbjct: 121 ASMEIHQALTRSSIIRNVLPRHEQGGVFAAEGYARSSGLPGVCIATSGPGATNLVSGLAD 180

Query: 189 ALLDSVPLVAITGQVPRGMMGNNAFQDIPIVEVTRSITKHNYLVLDVDDIPRIVSEAFFL 248
           ALLDSVPLVAITGQVPR M+G +AFQ+ PIVEVTRSITKHNYLVLDVDDIPRIVSEAFFL
Sbjct: 181 ALLDSVPLVAITGQVPRRMIGTDAFQETPIVEVTRSITKHNYLVLDVDDIPRIVSEAFFL 240

Query: 249 ATSGRPGPVLIDIPKDIQQQLAIPNWNQPIKLSAYLSTLPNPPNDAPLDQILRLISESKK 308
           A+SGRPGPVLIDIPKD+QQQLA+PNWNQP+KL  YLS LP PP D+ L+QILRLISESKK
Sbjct: 241 ASSGRPGPVLIDIPKDVQQQLAVPNWNQPMKLPGYLSRLPKPPTDSHLEQILRLISESKK 300

Query: 309 PVLYVGGGCLNSPDELRRFVGLTGIPVASTLMGLGAYPCNHELSLQMLGMHGTVYANYAV 368
           PVLYVGGGCLNS +ELRRFV LTGIPVASTLMGLGAYPC+ +LSLQMLGMHGTVYANYAV
Sbjct: 301 PVLYVGGGCLNSSEELRRFVKLTGIPVASTLMGLGAYPCSDDLSLQMLGMHGTVYANYAV 360

Query: 369 DKSDLLLAFGVRFDERVTGKLEAFASRAKIVHIDIDSYEIGRNKQPHVWVCGDVKLAMQR 428
           DKSDLLLAFGVRFD+RVTGKLEAFASRAKIVHIDIDS EIG+NKQPHV VCGDVKLA+Q 
Sbjct: 361 DKSDLLLAFGVRFDDRVTGKLEAFASRAKIVHIDIDSAEIGKNKQPHVSVCGDVKLALQG 420

Query: 429 MNRLMEENAEKLLHCDFSAWREELDGQKSKYPLSYKTFEEAIPPQYAIQVLDELTNGRAI 488
           MNRL+E+    +LH DFSAWREEL+ QKSKYPL++KTF+EAIPPQYAIQ+LDELTNG AI
Sbjct: 421 MNRLLEK--AHMLHFDFSAWREELNEQKSKYPLTFKTFDEAIPPQYAIQLLDELTNGEAI 480

Query: 489 ISTGVGQHQMWVAQFYKFKKPRQWLTSGGLGAMGFGLPAAMGAVVANPEAVVVDVDGDGS 548
           +STGVGQHQMW AQFYK+KKPRQWLTSGGLGAMGFGLPAAMGA VANP AVVVD+DGDGS
Sbjct: 481 VSTGVGQHQMWAAQFYKYKKPRQWLTSGGLGAMGFGLPAAMGAAVANPGAVVVDIDGDGS 540

Query: 549 FMMNIQELAAISVEKLPVKILLLNNQYLGMVLQWEDQFNKLGNRAQTYLGDPSKGSEIFP 608
           F+MN+QELA ISVEKLPVKILLLNNQ+LGMV+QWED+F K  NRA TYLG+PS  SEIFP
Sbjct: 541 FIMNVQELATISVEKLPVKILLLNNQHLGMVVQWEDRFYK-ANRAHTYLGNPSNESEIFP 600

Query: 609 NMLKFAEACGIPAARVTKKWEVKTAIKKMLETEGPYLLDVIIPHQEYVLPLIPSGGTFKD 657
           NMLKFAEACGIPAARVTKK E++ A+KKMLETEGPY+LDVI+PHQE+VLP+IPSGG FKD
Sbjct: 601 NMLKFAEACGIPAARVTKKAELRAAMKKMLETEGPYMLDVIVPHQEHVLPMIPSGGAFKD 660

[Close]

BLAST of Moc10g25860 vs. NCBI nr
Match: XP_023007605.1 (acetolactate synthase 2, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 1031.6 bits (2666), Expect = 3.0e-297
Identity = 532/669 (79.52%), Postives = 585/669 (87.44%), Query Frame = 0


Query: 9   MPSMAAMSFCTSISKYSSSSTSFKPSKPISPFFQSFSILTQNHTFFCRPIRICSSL---- 68
           M SMAA +   +   +S SS+S  P KP S F  SFS L  N TF  RP+R+CSS     
Sbjct: 1   MSSMAAATHPCASKSFSKSSSSVLP-KPFSNFHFSFSPLPHNPTFL-RPLRVCSSSLSNP 60

Query: 69  --SPSSV-------------PEIFIVSRFAADEPRKGADILIEALERQGVTSVFAYPGGA 128
              PSS              PEIF VSRF+ DEPRKGADIL+EALERQGVT+VFAYPGGA
Sbjct: 61  SPKPSSTAASATSVETSVISPEIF-VSRFSPDEPRKGADILVEALERQGVTNVFAYPGGA 120

Query: 129 SMEIHQALTRSAIIRDILPRHEQGGVFAAEGYARSSGLPGVCIATSGPGATNLVTGLADA 188
           SMEIHQALTRS+IIR++LPRHEQGGVFAAEGYARSSGLPGVCIATSGPGATNLV+GLADA
Sbjct: 121 SMEIHQALTRSSIIRNVLPRHEQGGVFAAEGYARSSGLPGVCIATSGPGATNLVSGLADA 180

Query: 189 LLDSVPLVAITGQVPRGMMGNNAFQDIPIVEVTRSITKHNYLVLDVDDIPRIVSEAFFLA 248
           LLDSVP+VAITGQVPR M+G +AFQ+ PIVEVTRSITKHNYLVLDV+DIPRIVSEAFFLA
Sbjct: 181 LLDSVPMVAITGQVPRRMIGTDAFQETPIVEVTRSITKHNYLVLDVEDIPRIVSEAFFLA 240

Query: 249 TSGRPGPVLIDIPKDIQQQLAIPNWNQPIKLSAYLSTLPNPPNDAPLDQILRLISESKKP 308
            SGRPGPVLIDIPKD+QQQLA+PNWNQP+KL  YLS LP PP D  L+QI+RLISESKKP
Sbjct: 241 ISGRPGPVLIDIPKDVQQQLAVPNWNQPMKLPGYLSRLPKPPMDYHLEQIVRLISESKKP 300

Query: 309 VLYVGGGCLNSPDELRRFVGLTGIPVASTLMGLGAYPCNHELSLQMLGMHGTVYANYAVD 368
           VLYVGGGCLNS  ELR+FV LTGIPVASTLMGLGA+PC+ +LSLQMLGMHGTVYANYAVD
Sbjct: 301 VLYVGGGCLNSSVELRQFVKLTGIPVASTLMGLGAFPCSDDLSLQMLGMHGTVYANYAVD 360

Query: 369 KSDLLLAFGVRFDERVTGKLEAFASRAKIVHIDIDSYEIGRNKQPHVWVCGDVKLAMQRM 428
           KSDLLLAFGVRFD+RVTGKLEAFASRAKIVH+DIDS EIG+NKQPHV VC DVKL +QRM
Sbjct: 361 KSDLLLAFGVRFDDRVTGKLEAFASRAKIVHVDIDSAEIGKNKQPHVSVCSDVKLVLQRM 420

Query: 429 NRLMEENAEKLLHCDFSAWREELDGQKSKYPLSYKTFEEAIPPQYAIQVLDELTNGRAII 488
           NRL+ EN+EK LH DFSAWREEL+ QKSKYPL++KTF+EAIPPQYAI VLDELTNG AII
Sbjct: 421 NRLL-ENSEK-LHFDFSAWREELNEQKSKYPLTFKTFDEAIPPQYAILVLDELTNGEAII 480

Query: 489 STGVGQHQMWVAQFYKFKKPRQWLTSGGLGAMGFGLPAAMGAVVANPEAVVVDVDGDGSF 548
           STGVGQHQMW AQFYK+KKPRQWLTSGGLGAMGFGLPAAMGA VANP+AVVVD+DGDGSF
Sbjct: 481 STGVGQHQMWAAQFYKYKKPRQWLTSGGLGAMGFGLPAAMGAAVANPDAVVVDIDGDGSF 540

Query: 549 MMNIQELAAISVEKLPVKILLLNNQYLGMVLQWEDQFNKLGNRAQTYLGDPSKGSEIFPN 608
           MMNIQELA ISVEKLPVKILLLNNQ+LGMV+QWED+F K  NRA TYLGDPS  SEIFPN
Sbjct: 541 MMNIQELATISVEKLPVKILLLNNQHLGMVVQWEDRFYK-ANRAHTYLGDPSNESEIFPN 600

Query: 609 MLKFAEACGIPAARVTKKWEVKTAIKKMLETEGPYLLDVIIPHQEYVLPLIPSGGTFKDV 659
           MLKFAEACG+ AARVTK+ +++ AIKKMLE+EGPYLLDVI+PHQE+VLP+IPSGG FKDV
Sbjct: 601 MLKFAEACGVRAARVTKRADLRAAIKKMLESEGPYLLDVIVPHQEHVLPMIPSGGAFKDV 660

[Close]

BLAST of Moc10g25860 vs. ExPASy Swiss-Prot
Match: P27819 (Acetolactate synthase 3, chloroplastic OS=Brassica napus OX=3708 PE=3 SV=1)

HSP 1 Score: 960.3 bits (2481), Expect = 1.1e-278
Identity = 491/653 (75.19%), Postives = 559/653 (85.60%), Query Frame = 0


Query: 15  MSFCTSISKYS-SSSTSFKPSKPISPFFQSFSILTQN-HTFFCRPIRICSSL-SPSSV-P 74
           M+  TS S  S ++  S K   PIS F   FS+  Q   +   RP+ I + L SP +V P
Sbjct: 1   MAAATSSSPISLTAKPSSKSPLPISRFSLPFSLTPQKPSSRLHRPLAISAVLNSPVNVAP 60

Query: 75  E-----IFIVSRFAADEPRKGADILIEALERQGVTSVFAYPGGASMEIHQALTRSAIIRD 134
           E        +SR+A DEPRKGADIL+EALERQGV +VFAYPGGASMEIHQALTRS+ IR+
Sbjct: 61  EKTDKIKTFISRYAPDEPRKGADILVEALERQGVETVFAYPGGASMEIHQALTRSSTIRN 120

Query: 135 ILPRHEQGGVFAAEGYARSSGLPGVCIATSGPGATNLVTGLADALLDSVPLVAITGQVPR 194
           +LPRHEQGGVFAAEGYARSSG PG+CIATSGPGATNLV+GLADA+LDSVPLVAITGQVPR
Sbjct: 121 VLPRHEQGGVFAAEGYARSSGKPGICIATSGPGATNLVSGLADAMLDSVPLVAITGQVPR 180

Query: 195 GMMGNNAFQDIPIVEVTRSITKHNYLVLDVDDIPRIVSEAFFLATSGRPGPVLIDIPKDI 254
            M+G +AFQ+ PIVEVTRSITKHNYLV+DVDDIPRIV EAFFLATSGRPGPVL+D+PKDI
Sbjct: 181 RMIGTDAFQETPIVEVTRSITKHNYLVMDVDDIPRIVQEAFFLATSGRPGPVLVDVPKDI 240

Query: 255 QQQLAIPNWNQPIKLSAYLSTLPNPPNDAPLDQILRLISESKKPVLYVGGGCLNSPDELR 314
           QQQLAIPNW+QP++L  Y+S LP PP  + L QI+RLISESK+PVLYVGGG LNS +EL 
Sbjct: 241 QQQLAIPNWDQPMRLPGYMSRLPQPPEVSQLGQIVRLISESKRPVLYVGGGSLNSSEELG 300

Query: 315 RFVGLTGIPVASTLMGLGAYPCNHELSLQMLGMHGTVYANYAVDKSDLLLAFGVRFDERV 374
           RFV LTGIPVASTLMGLG+YPCN ELSLQMLGMHGTVYANYAV+ SDLLLAFGVRFD+RV
Sbjct: 301 RFVELTGIPVASTLMGLGSYPCNDELSLQMLGMHGTVYANYAVEHSDLLLAFGVRFDDRV 360

Query: 375 TGKLEAFASRAKIVHIDIDSYEIGRNKQPHVWVCGDVKLAMQRMNRLMEENAEKLLHCDF 434
           TGKLEAFASRAKIVHIDIDS EIG+NK PHV VCGDVKLA+Q MN+++E  AE+ L  DF
Sbjct: 361 TGKLEAFASRAKIVHIDIDSAEIGKNKTPHVSVCGDVKLALQGMNKVLENRAEE-LKLDF 420

Query: 435 SAWREELDGQKSKYPLSYKTFEEAIPPQYAIQVLDELTNGRAIISTGVGQHQMWVAQFYK 494
             WR EL  QK K+PLS+KTF EAIPPQYAIQVLDELT G+AIISTGVGQHQMW AQFYK
Sbjct: 421 GVWRSELSEQKQKFPLSFKTFGEAIPPQYAIQVLDELTQGKAIISTGVGQHQMWAAQFYK 480

Query: 495 FKKPRQWLTSGGLGAMGFGLPAAMGAVVANPEAVVVDVDGDGSFMMNIQELAAISVEKLP 554
           ++KPRQWL+S GLGAMGFGLPAA+GA VANP+A+VVD+DGDGSF+MN+QELA I VE LP
Sbjct: 481 YRKPRQWLSSSGLGAMGFGLPAAIGASVANPDAIVVDIDGDGSFIMNVQELATIRVENLP 540

Query: 555 VKILLLNNQYLGMVLQWEDQFNKLGNRAQTYLGDPSKGSEIFPNMLKFAEACGIPAARVT 614
           VKILLLNNQ+LGMV+QWED+F K  NRA TYLGDP++ +EIFPNML+FA ACGIPAARVT
Sbjct: 541 VKILLLNNQHLGMVMQWEDRFYK-ANRAHTYLGDPARENEIFPNMLQFAGACGIPAARVT 600

Query: 615 KKWEVKTAIKKMLETEGPYLLDVIIPHQEYVLPLIPSGGTFKDVITEGDGRSQ 659
           KK E++ AI+ ML+T GPYLLDVI PHQE+VLP+IPSGGTFKDVITEGDGR++
Sbjct: 601 KKEELREAIQTMLDTPGPYLLDVICPHQEHVLPMIPSGGTFKDVITEGDGRTK 651

[Close]

BLAST of Moc10g25860 vs. ExPASy Swiss-Prot
Match: P27818 (Acetolactate synthase 1, chloroplastic OS=Brassica napus OX=3708 PE=3 SV=1)

HSP 1 Score: 959.5 bits (2479), Expect = 1.9e-278
Identity = 491/656 (74.85%), Postives = 560/656 (85.37%), Query Frame = 0


Query: 15  MSFCTSISKYS-SSSTSFKPSKPISPFFQSFSILTQ-NHTFFCRPIRICSSL-SPSSV-- 74
           M+  TS S  S ++  S K   PIS F   FS+  Q + +   RP+ I + L SP +V  
Sbjct: 1   MAAATSSSPISLTAKPSSKSPLPISRFSLPFSLTPQKDSSRLHRPLAISAVLNSPVNVAP 60

Query: 75  --PE-----IFIVSRFAADEPRKGADILIEALERQGVTSVFAYPGGASMEIHQALTRSAI 134
             PE        VSR+A DEPRKGADIL+EALERQGV +VFAYPGGASMEIHQALTRS+ 
Sbjct: 61  PSPEKTDKNKTFVSRYAPDEPRKGADILVEALERQGVETVFAYPGGASMEIHQALTRSST 120

Query: 135 IRDILPRHEQGGVFAAEGYARSSGLPGVCIATSGPGATNLVTGLADALLDSVPLVAITGQ 194
           IR++LPRHEQGGVFAAEGYARSSG PG+CIATSGPGATNLV+GLADA+LDSVPLVAITGQ
Sbjct: 121 IRNVLPRHEQGGVFAAEGYARSSGKPGICIATSGPGATNLVSGLADAMLDSVPLVAITGQ 180

Query: 195 VPRGMMGNNAFQDIPIVEVTRSITKHNYLVLDVDDIPRIVSEAFFLATSGRPGPVLIDIP 254
           VPR M+G +AFQ+ PIVEVTRSITKHNYLV+DVDDIPRIV EAFFLATSGRPGPVL+D+P
Sbjct: 181 VPRRMIGTDAFQETPIVEVTRSITKHNYLVMDVDDIPRIVQEAFFLATSGRPGPVLVDVP 240

Query: 255 KDIQQQLAIPNWNQPIKLSAYLSTLPNPPNDAPLDQILRLISESKKPVLYVGGGCLNSPD 314
           KDIQQQLAIPNW+QP++L  Y+S LP PP  + L QI+RLISESK+PVLYVGGG LNS +
Sbjct: 241 KDIQQQLAIPNWDQPMRLPGYMSRLPQPPEVSQLGQIVRLISESKRPVLYVGGGSLNSSE 300

Query: 315 ELRRFVGLTGIPVASTLMGLGAYPCNHELSLQMLGMHGTVYANYAVDKSDLLLAFGVRFD 374
           EL RFV LTGIPVASTLMGLG+YPCN ELSLQMLGMHGTVYANYAV+ SDLLLAFGVRFD
Sbjct: 301 ELGRFVELTGIPVASTLMGLGSYPCNDELSLQMLGMHGTVYANYAVEHSDLLLAFGVRFD 360

Query: 375 ERVTGKLEAFASRAKIVHIDIDSYEIGRNKQPHVWVCGDVKLAMQRMNRLMEENAEKLLH 434
           +RVTGKLEAFASRAKIVHIDIDS EIG+NK PHV VCGDVKLA+Q MN+++E  AE+ L 
Sbjct: 361 DRVTGKLEAFASRAKIVHIDIDSAEIGKNKTPHVSVCGDVKLALQGMNKVLENRAEE-LK 420

Query: 435 CDFSAWREELDGQKSKYPLSYKTFEEAIPPQYAIQVLDELTNGRAIISTGVGQHQMWVAQ 494
            DF  WR EL  QK K+PLS+KTF EAIPPQYAIQ+LDELT G+AIISTGVGQHQMW AQ
Sbjct: 421 LDFGVWRSELSEQKQKFPLSFKTFGEAIPPQYAIQILDELTEGKAIISTGVGQHQMWAAQ 480

Query: 495 FYKFKKPRQWLTSGGLGAMGFGLPAAMGAVVANPEAVVVDVDGDGSFMMNIQELAAISVE 554
           FYK++KPRQWL+S GLGAMGFGLPAA+GA VANP+A+VVD+DGDGSF+MN+QELA I VE
Sbjct: 481 FYKYRKPRQWLSSSGLGAMGFGLPAAIGASVANPDAIVVDIDGDGSFIMNVQELATIRVE 540

Query: 555 KLPVKILLLNNQYLGMVLQWEDQFNKLGNRAQTYLGDPSKGSEIFPNMLKFAEACGIPAA 614
            LPVKILLLNNQ+LGMV+QWED+F K  NRA TYLGDP++ +EIFPNML+FA ACGIPAA
Sbjct: 541 NLPVKILLLNNQHLGMVMQWEDRFYK-ANRAHTYLGDPARENEIFPNMLQFAGACGIPAA 600

Query: 615 RVTKKWEVKTAIKKMLETEGPYLLDVIIPHQEYVLPLIPSGGTFKDVITEGDGRSQ 659
           RVTKK E++ AI+ ML+T GPYLLDVI PHQE+VLP+IPSGGTFKDVITEGDGR++
Sbjct: 601 RVTKKEELREAIQTMLDTPGPYLLDVICPHQEHVLPMIPSGGTFKDVITEGDGRTK 654

[Close]

BLAST of Moc10g25860 vs. ExPASy Swiss-Prot
Match: P17597 (Acetolactate synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ALS PE=1 SV=1)

HSP 1 Score: 956.1 bits (2470), Expect = 2.1e-277
Identity = 491/671 (73.17%), Postives = 556/671 (82.86%), Query Frame = 0


Query: 12  MAAMSFCTSISKYSSSSTSFKPSK-----PISPFFQSFSILTQNHTFFCRPIRICSSLSP 71
           MAA +  T+ S   S ST   PS      PIS F   FS L  N +      R   S SP
Sbjct: 1   MAAATTTTTTSSSISFSTKPSPSSSKSPLPISRFSLPFS-LNPNKSSSSSRRRGIKSSSP 60

Query: 72  SSV---------------------PEIFIVSRFAADEPRKGADILIEALERQGVTSVFAY 131
           SS+                     PE FI SRFA D+PRKGADIL+EALERQGV +VFAY
Sbjct: 61  SSISAVLNTTTNVTTTPSPTKPTKPETFI-SRFAPDQPRKGADILVEALERQGVETVFAY 120

Query: 132 PGGASMEIHQALTRSAIIRDILPRHEQGGVFAAEGYARSSGLPGVCIATSGPGATNLVTG 191
           PGGASMEIHQALTRS+ IR++LPRHEQGGVFAAEGYARSSG PG+CIATSGPGATNLV+G
Sbjct: 121 PGGASMEIHQALTRSSSIRNVLPRHEQGGVFAAEGYARSSGKPGICIATSGPGATNLVSG 180

Query: 192 LADALLDSVPLVAITGQVPRGMMGNNAFQDIPIVEVTRSITKHNYLVLDVDDIPRIVSEA 251
           LADALLDSVPLVAITGQVPR M+G +AFQ+ PIVEVTRSITKHNYLV+DV+DIPRI+ EA
Sbjct: 181 LADALLDSVPLVAITGQVPRRMIGTDAFQETPIVEVTRSITKHNYLVMDVEDIPRIIEEA 240

Query: 252 FFLATSGRPGPVLIDIPKDIQQQLAIPNWNQPIKLSAYLSTLPNPPNDAPLDQILRLISE 311
           FFLATSGRPGPVL+D+PKDIQQQLAIPNW Q ++L  Y+S +P PP D+ L+QI+RLISE
Sbjct: 241 FFLATSGRPGPVLVDVPKDIQQQLAIPNWEQAMRLPGYMSRMPKPPEDSHLEQIVRLISE 300

Query: 312 SKKPVLYVGGGCLNSPDELRRFVGLTGIPVASTLMGLGAYPCNHELSLQMLGMHGTVYAN 371
           SKKPVLYVGGGCLNS DEL RFV LTGIPVASTLMGLG+YPC+ ELSL MLGMHGTVYAN
Sbjct: 301 SKKPVLYVGGGCLNSSDELGRFVELTGIPVASTLMGLGSYPCDDELSLHMLGMHGTVYAN 360

Query: 372 YAVDKSDLLLAFGVRFDERVTGKLEAFASRAKIVHIDIDSYEIGRNKQPHVWVCGDVKLA 431
           YAV+ SDLLLAFGVRFD+RVTGKLEAFASRAKIVHIDIDS EIG+NK PHV VCGDVKLA
Sbjct: 361 YAVEHSDLLLAFGVRFDDRVTGKLEAFASRAKIVHIDIDSAEIGKNKTPHVSVCGDVKLA 420

Query: 432 MQRMNRLMEENAEKLLHCDFSAWREELDGQKSKYPLSYKTFEEAIPPQYAIQVLDELTNG 491
           +Q MN+++E  AE+ L  DF  WR EL+ QK K+PLS+KTF EAIPPQYAI+VLDELT+G
Sbjct: 421 LQGMNKVLENRAEE-LKLDFGVWRNELNVQKQKFPLSFKTFGEAIPPQYAIKVLDELTDG 480

Query: 492 RAIISTGVGQHQMWVAQFYKFKKPRQWLTSGGLGAMGFGLPAAMGAVVANPEAVVVDVDG 551
           +AIISTGVGQHQMW AQFY +KKPRQWL+SGGLGAMGFGLPAA+GA VANP+A+VVD+DG
Sbjct: 481 KAIISTGVGQHQMWAAQFYNYKKPRQWLSSGGLGAMGFGLPAAIGASVANPDAIVVDIDG 540

Query: 552 DGSFMMNIQELAAISVEKLPVKILLLNNQYLGMVLQWEDQFNKLGNRAQTYLGDPSKGSE 611
           DGSF+MN+QELA I VE LPVK+LLLNNQ+LGMV+QWED+F K  NRA T+LGDP++  E
Sbjct: 541 DGSFIMNVQELATIRVENLPVKVLLLNNQHLGMVMQWEDRFYK-ANRAHTFLGDPAQEDE 600

Query: 612 IFPNMLKFAEACGIPAARVTKKWEVKTAIKKMLETEGPYLLDVIIPHQEYVLPLIPSGGT 657
           IFPNML FA ACGIPAARVTKK +++ AI+ ML+T GPYLLDVI PHQE+VLP+IPSGGT
Sbjct: 601 IFPNMLLFAAACGIPAARVTKKADLREAIQTMLDTPGPYLLDVICPHQEHVLPMIPSGGT 660

[Close]

BLAST of Moc10g25860 vs. ExPASy Swiss-Prot
Match: P09114 (Acetolactate synthase 2, chloroplastic OS=Nicotiana tabacum OX=4097 GN=ALS SURB PE=1 SV=1)

HSP 1 Score: 943.3 bits (2437), Expect = 1.4e-273
Identity = 462/600 (77.00%), Postives = 528/600 (88.00%), Query Frame = 0


Query: 58  IRICSSLSPSSVPEIFIVSRFAADEPRKGADILIEALERQGVTSVFAYPGGASMEIHQAL 117
           I     +S +   E F VSRFA DEPRKG+D+L+EALER+GVT VFAYPGGASMEIHQAL
Sbjct: 66  ISTTQKVSETQKAETF-VSRFAPDEPRKGSDVLVEALEREGVTDVFAYPGGASMEIHQAL 125

Query: 118 TRSAIIRDILPRHEQGGVFAAEGYARSSGLPGVCIATSGPGATNLVTGLADALLDSVPLV 177
           TRS+IIR++LPRHEQGGVFAAEGYAR++G PGVCIATSGPGATNLV+GLADALLDSVP+V
Sbjct: 126 TRSSIIRNVLPRHEQGGVFAAEGYARATGFPGVCIATSGPGATNLVSGLADALLDSVPIV 185

Query: 178 AITGQVPRGMMGNNAFQDIPIVEVTRSITKHNYLVLDVDDIPRIVSEAFFLATSGRPGPV 237
           AITGQVPR M+G +AFQ+ PIVEVTRSITKHNYLV+DV+DIPR+V EAFFLA SGRPGPV
Sbjct: 186 AITGQVPRRMIGTDAFQETPIVEVTRSITKHNYLVMDVEDIPRVVREAFFLARSGRPGPV 245

Query: 238 LIDIPKDIQQQLAIPNWNQPIKLSAYLSTLPNPPNDAPLDQILRLISESKKPVLYVGGGC 297
           LID+PKDIQQQL IP+W+QP++L  Y+S LP  PN+  L+QI+RLISESKKPVLYVGGGC
Sbjct: 246 LIDVPKDIQQQLVIPDWDQPMRLPGYMSRLPKLPNEMLLEQIVRLISESKKPVLYVGGGC 305

Query: 298 LNSPDELRRFVGLTGIPVASTLMGLGAYPCNHELSLQMLGMHGTVYANYAVDKSDLLLAF 357
             S +ELRRFV LTGIPVASTLMGLGA+P   ELSL MLGMHGTVYANYAVD SDLLLAF
Sbjct: 306 SQSSEELRRFVELTGIPVASTLMGLGAFPTGDELSLSMLGMHGTVYANYAVDSSDLLLAF 365

Query: 358 GVRFDERVTGKLEAFASRAKIVHIDIDSYEIGRNKQPHVWVCGDVKLAMQRMNRLMEENA 417
           GVRFD+RVTGKLEAFASRAKIVHIDIDS EIG+NKQPHV +C D+KLA+Q +N ++E   
Sbjct: 366 GVRFDDRVTGKLEAFASRAKIVHIDIDSAEIGKNKQPHVSICADIKLALQGLNSILESKE 425

Query: 418 EKLLHCDFSAWREELDGQKSKYPLSYKTFEEAIPPQYAIQVLDELTNGRAIISTGVGQHQ 477
            K L  DFSAWR+EL  QK KYPL++KTF +AIPPQYAIQVLDELTNG AIISTGVGQHQ
Sbjct: 426 GK-LKLDFSAWRQELTVQKVKYPLNFKTFGDAIPPQYAIQVLDELTNGSAIISTGVGQHQ 485

Query: 478 MWVAQFYKFKKPRQWLTSGGLGAMGFGLPAAMGAVVANPEAVVVDVDGDGSFMMNIQELA 537
           MW AQ+YK++KPRQWLTSGGLGAMGFGLPAA+GA V  P+ VVVD+DGDGSF+MN+QELA
Sbjct: 486 MWAAQYYKYRKPRQWLTSGGLGAMGFGLPAAIGAAVGRPDEVVVDIDGDGSFIMNVQELA 545

Query: 538 AISVEKLPVKILLLNNQYLGMVLQWEDQFNKLGNRAQTYLGDPSKGSEIFPNMLKFAEAC 597
            I VE LPVKI+LLNNQ+LGMV+QWED+F K  NRA TYLG+PS  +EIFPNMLKFAEAC
Sbjct: 546 TIKVENLPVKIMLLNNQHLGMVVQWEDRFYK-ANRAHTYLGNPSNEAEIFPNMLKFAEAC 605

Query: 598 GIPAARVTKKWEVKTAIKKMLETEGPYLLDVIIPHQEYVLPLIPSGGTFKDVITEGDGRS 657
           G+PAARVT + +++ AI+KML+T GPYLLDVI+PHQE+VLP+IPSGG FKDVITEGDGRS
Sbjct: 606 GVPAARVTHRDDLRAAIQKMLDTPGPYLLDVIVPHQEHVLPMIPSGGAFKDVITEGDGRS 662

[Close]

BLAST of Moc10g25860 vs. ExPASy Swiss-Prot
Match: P09342 (Acetolactate synthase 1, chloroplastic OS=Nicotiana tabacum OX=4097 GN=ALS SURA PE=1 SV=1)

HSP 1 Score: 937.2 bits (2421), Expect = 1.0e-271
Identity = 471/663 (71.04%), Postives = 550/663 (82.96%), Query Frame = 0


Query: 10  PSMAAMSFCTSISKYSSSSTSFKPSK--------------PISPFFQSFSILTQNHTF-F 69
           PS ++ +F  ++S  SS+S++  P                P+        I +Q   F  
Sbjct: 6   PSPSSSAFSKTLSPSSSTSSTLLPRSTFPFPHHPHKTTPPPLHLTHTHIHIHSQRRRFTI 65

Query: 70  CRPIRICSSLSPSSVPEIFIVSRFAADEPRKGADILIEALERQGVTSVFAYPGGASMEIH 129
              I     +S +   E F VSRFA DEPRKG+D+L+EALER+GVT VFAYPGGASMEIH
Sbjct: 66  SNVISTNQKVSQTEKTETF-VSRFAPDEPRKGSDVLVEALEREGVTDVFAYPGGASMEIH 125

Query: 130 QALTRSAIIRDILPRHEQGGVFAAEGYARSSGLPGVCIATSGPGATNLVTGLADALLDSV 189
           QALTRS+IIR++LPRHEQGGVFAAEGYAR++G PGVCIATSGPGATNLV+GLADALLDSV
Sbjct: 126 QALTRSSIIRNVLPRHEQGGVFAAEGYARATGFPGVCIATSGPGATNLVSGLADALLDSV 185

Query: 190 PLVAITGQVPRGMMGNNAFQDIPIVEVTRSITKHNYLVLDVDDIPRIVSEAFFLATSGRP 249
           P+VAITGQVPR M+G +AFQ+ PIVEVTRSITKHNYLV+DV+DIPR+V EAFFLA SGRP
Sbjct: 186 PIVAITGQVPRRMIGTDAFQETPIVEVTRSITKHNYLVMDVEDIPRVVREAFFLARSGRP 245

Query: 250 GPVLIDIPKDIQQQLAIPNWNQPIKLSAYLSTLPNPPNDAPLDQILRLISESKKPVLYVG 309
           GP+LID+PKDIQQQL IP+W+QP++L  Y+S LP  PN+  L+QI+RLISESKKPVLYVG
Sbjct: 246 GPILIDVPKDIQQQLVIPDWDQPMRLPGYMSRLPKLPNEMLLEQIVRLISESKKPVLYVG 305

Query: 310 GGCLNSPDELRRFVGLTGIPVASTLMGLGAYPCNHELSLQMLGMHGTVYANYAVDKSDLL 369
           GGC  S ++LRRFV LTGIPVASTLMGLGA+P   ELSL MLGMHGTVYANYAVD SDLL
Sbjct: 306 GGCSQSSEDLRRFVELTGIPVASTLMGLGAFPTGDELSLSMLGMHGTVYANYAVDSSDLL 365

Query: 370 LAFGVRFDERVTGKLEAFASRAKIVHIDIDSYEIGRNKQPHVWVCGDVKLAMQRMNRLME 429
           LAFGVRFD+RVTGKLEAFASRAKIVHIDIDS EIG+NKQPHV +C D+KLA+Q +N ++E
Sbjct: 366 LAFGVRFDDRVTGKLEAFASRAKIVHIDIDSAEIGKNKQPHVSICADIKLALQGLNSILE 425

Query: 430 ENAEKLLHCDFSAWREELDGQKSKYPLSYKTFEEAIPPQYAIQVLDELTNGRAIISTGVG 489
               K L  DFSAWR+EL  QK K+PL++KTF +AIPPQYAIQVLDELTNG AIISTGVG
Sbjct: 426 SKEGK-LKLDFSAWRQELTEQKVKHPLNFKTFGDAIPPQYAIQVLDELTNGNAIISTGVG 485

Query: 490 QHQMWVAQFYKFKKPRQWLTSGGLGAMGFGLPAAMGAVVANPEAVVVDVDGDGSFMMNIQ 549
           QHQMW AQ+YK++KPRQWLTSGGLGAMGFGLPAA+GA V  P+ VVVD+DGDGSF+MN+Q
Sbjct: 486 QHQMWAAQYYKYRKPRQWLTSGGLGAMGFGLPAAIGAAVGRPDEVVVDIDGDGSFIMNVQ 545

Query: 550 ELAAISVEKLPVKILLLNNQYLGMVLQWEDQFNKLGNRAQTYLGDPSKGSEIFPNMLKFA 609
           ELA I VE LPVKI+LLNNQ+LGMV+QWED+F K  NRA TYLG+PS  +EIFPNMLKFA
Sbjct: 546 ELATIKVENLPVKIMLLNNQHLGMVVQWEDRFYK-ANRAHTYLGNPSNEAEIFPNMLKFA 605

Query: 610 EACGIPAARVTKKWEVKTAIKKMLETEGPYLLDVIIPHQEYVLPLIPSGGTFKDVITEGD 658
           EACG+PAARVT + +++ AI+KML+T GPYLLDVI+PHQE+VLP+IPSGG FKDVITEGD
Sbjct: 606 EACGVPAARVTHRDDLRAAIQKMLDTPGPYLLDVIVPHQEHVLPMIPSGGAFKDVITEGD 665

[Close]

BLAST of Moc10g25860 vs. ExPASy TrEMBL
Match: A0A6J1CPD6 (Acetolactate synthase OS=Momordica charantia OX=3673 GN=LOC111013340 PE=3 SV=1)

HSP 1 Score: 1316.6 bits (3406), Expect = 0.0e+00
Identity = 659/659 (100.00%), Postives = 659/659 (100.00%), Query Frame = 0


Query: 1   MLHLASFSMPSMAAMSFCTSISKYSSSSTSFKPSKPISPFFQSFSILTQNHTFFCRPIRI 60
           MLHLASFSMPSMAAMSFCTSISKYSSSSTSFKPSKPISPFFQSFSILTQNHTFFCRPIRI
Sbjct: 1   MLHLASFSMPSMAAMSFCTSISKYSSSSTSFKPSKPISPFFQSFSILTQNHTFFCRPIRI 60

Query: 61  CSSLSPSSVPEIFIVSRFAADEPRKGADILIEALERQGVTSVFAYPGGASMEIHQALTRS 120
           CSSLSPSSVPEIFIVSRFAADEPRKGADILIEALERQGVTSVFAYPGGASMEIHQALTRS
Sbjct: 61  CSSLSPSSVPEIFIVSRFAADEPRKGADILIEALERQGVTSVFAYPGGASMEIHQALTRS 120

Query: 121 AIIRDILPRHEQGGVFAAEGYARSSGLPGVCIATSGPGATNLVTGLADALLDSVPLVAIT 180
           AIIRDILPRHEQGGVFAAEGYARSSGLPGVCIATSGPGATNLVTGLADALLDSVPLVAIT
Sbjct: 121 AIIRDILPRHEQGGVFAAEGYARSSGLPGVCIATSGPGATNLVTGLADALLDSVPLVAIT 180

Query: 181 GQVPRGMMGNNAFQDIPIVEVTRSITKHNYLVLDVDDIPRIVSEAFFLATSGRPGPVLID 240
           GQVPRGMMGNNAFQDIPIVEVTRSITKHNYLVLDVDDIPRIVSEAFFLATSGRPGPVLID
Sbjct: 181 GQVPRGMMGNNAFQDIPIVEVTRSITKHNYLVLDVDDIPRIVSEAFFLATSGRPGPVLID 240

Query: 241 IPKDIQQQLAIPNWNQPIKLSAYLSTLPNPPNDAPLDQILRLISESKKPVLYVGGGCLNS 300
           IPKDIQQQLAIPNWNQPIKLSAYLSTLPNPPNDAPLDQILRLISESKKPVLYVGGGCLNS
Sbjct: 241 IPKDIQQQLAIPNWNQPIKLSAYLSTLPNPPNDAPLDQILRLISESKKPVLYVGGGCLNS 300

Query: 301 PDELRRFVGLTGIPVASTLMGLGAYPCNHELSLQMLGMHGTVYANYAVDKSDLLLAFGVR 360
           PDELRRFVGLTGIPVASTLMGLGAYPCNHELSLQMLGMHGTVYANYAVDKSDLLLAFGVR
Sbjct: 301 PDELRRFVGLTGIPVASTLMGLGAYPCNHELSLQMLGMHGTVYANYAVDKSDLLLAFGVR 360

Query: 361 FDERVTGKLEAFASRAKIVHIDIDSYEIGRNKQPHVWVCGDVKLAMQRMNRLMEENAEKL 420
           FDERVTGKLEAFASRAKIVHIDIDSYEIGRNKQPHVWVCGDVKLAMQRMNRLMEENAEKL
Sbjct: 361 FDERVTGKLEAFASRAKIVHIDIDSYEIGRNKQPHVWVCGDVKLAMQRMNRLMEENAEKL 420

Query: 421 LHCDFSAWREELDGQKSKYPLSYKTFEEAIPPQYAIQVLDELTNGRAIISTGVGQHQMWV 480
           LHCDFSAWREELDGQKSKYPLSYKTFEEAIPPQYAIQVLDELTNGRAIISTGVGQHQMWV
Sbjct: 421 LHCDFSAWREELDGQKSKYPLSYKTFEEAIPPQYAIQVLDELTNGRAIISTGVGQHQMWV 480

Query: 481 AQFYKFKKPRQWLTSGGLGAMGFGLPAAMGAVVANPEAVVVDVDGDGSFMMNIQELAAIS 540
           AQFYKFKKPRQWLTSGGLGAMGFGLPAAMGAVVANPEAVVVDVDGDGSFMMNIQELAAIS
Sbjct: 481 AQFYKFKKPRQWLTSGGLGAMGFGLPAAMGAVVANPEAVVVDVDGDGSFMMNIQELAAIS 540

Query: 541 VEKLPVKILLLNNQYLGMVLQWEDQFNKLGNRAQTYLGDPSKGSEIFPNMLKFAEACGIP 600
           VEKLPVKILLLNNQYLGMVLQWEDQFNKLGNRAQTYLGDPSKGSEIFPNMLKFAEACGIP
Sbjct: 541 VEKLPVKILLLNNQYLGMVLQWEDQFNKLGNRAQTYLGDPSKGSEIFPNMLKFAEACGIP 600

Query: 601 AARVTKKWEVKTAIKKMLETEGPYLLDVIIPHQEYVLPLIPSGGTFKDVITEGDGRSQS 660
           AARVTKKWEVKTAIKKMLETEGPYLLDVIIPHQEYVLPLIPSGGTFKDVITEGDGRSQS
Sbjct: 601 AARVTKKWEVKTAIKKMLETEGPYLLDVIIPHQEYVLPLIPSGGTFKDVITEGDGRSQS 659

[Close]

BLAST of Moc10g25860 vs. ExPASy TrEMBL
Match: A0A5A7URM3 (Acetolactate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G00520 PE=3 SV=1)

HSP 1 Score: 1037.7 bits (2682), Expect = 2.1e-299
Identity = 538/667 (80.66%), Postives = 588/667 (88.16%), Query Frame = 0


Query: 12  MAAMSFCTS--ISKYSSSSTSFKPSKPISPFFQSFSILTQNHTFFCRPIRICS-SLS-PS 71
           M++M+  TS   SK  S S+SF  +KP S F  SFS L  N T F RP+RICS SLS PS
Sbjct: 1   MSSMAAATSPCASKSFSKSSSFNLTKPFSRFDLSFSSLPHNPT-FRRPLRICSTSLSNPS 60

Query: 72  SVP------------------EIFIVSRFAADEPRKGADILIEALERQGVTSVFAYPGGA 131
             P                  +IF VSRFAADEPRKGADIL+EALERQGVT+VFAYPGGA
Sbjct: 61  PKPSSTAASAAAVETSSIASSDIF-VSRFAADEPRKGADILVEALERQGVTNVFAYPGGA 120

Query: 132 SMEIHQALTRSAIIRDILPRHEQGGVFAAEGYARSSGLPGVCIATSGPGATNLVTGLADA 191
           SMEIHQALTRS+IIR++LPRHEQGGVFAAEGYARSSGLPGVCIATSGPGATNLV+GLADA
Sbjct: 121 SMEIHQALTRSSIIRNVLPRHEQGGVFAAEGYARSSGLPGVCIATSGPGATNLVSGLADA 180

Query: 192 LLDSVPLVAITGQVPRGMMGNNAFQDIPIVEVTRSITKHNYLVLDVDDIPRIVSEAFFLA 251
           LLDSVPLVAITGQVPR M+G +AFQ+ PIVEVTRSITKHNYLVLDVDDIPRIVSEAFFLA
Sbjct: 181 LLDSVPLVAITGQVPRRMIGTDAFQETPIVEVTRSITKHNYLVLDVDDIPRIVSEAFFLA 240

Query: 252 TSGRPGPVLIDIPKDIQQQLAIPNWNQPIKLSAYLSTLPNPPNDAPLDQILRLISESKKP 311
            SGRPGPVLIDIPKD+QQQLA+PNWNQP+KL  YLS LP PP D+ L+QILRLISESKKP
Sbjct: 241 ISGRPGPVLIDIPKDVQQQLAVPNWNQPMKLPGYLSRLPKPPIDSHLEQILRLISESKKP 300

Query: 312 VLYVGGGCLNSPDELRRFVGLTGIPVASTLMGLGAYPCNHELSLQMLGMHGTVYANYAVD 371
           VLYVGGGCLNS +ELRRFV  TGIPVASTLMGLGAYPC  +LSLQMLGMHGTVYANYAVD
Sbjct: 301 VLYVGGGCLNSSEELRRFVKFTGIPVASTLMGLGAYPCADDLSLQMLGMHGTVYANYAVD 360

Query: 372 KSDLLLAFGVRFDERVTGKLEAFASRAKIVHIDIDSYEIGRNKQPHVWVCGDVKLAMQRM 431
           KSDLLLAFGVRFD+RVTGKLEAFASRAKIVHIDIDS EIG+NKQPHV VCGDVKLA+Q M
Sbjct: 361 KSDLLLAFGVRFDDRVTGKLEAFASRAKIVHIDIDSAEIGKNKQPHVSVCGDVKLALQGM 420

Query: 432 NRLMEENAEKLLHCDFSAWREELDGQKSKYPLSYKTFEEAIPPQYAIQVLDELTNGRAII 491
           NRL+E+   ++LH DFSAWREEL+ QKSKYPL++KTF+EAIPPQYAIQ+LDELTNG AII
Sbjct: 421 NRLLEK--AQMLHFDFSAWREELNEQKSKYPLTFKTFDEAIPPQYAIQLLDELTNGEAII 480

Query: 492 STGVGQHQMWVAQFYKFKKPRQWLTSGGLGAMGFGLPAAMGAVVANPEAVVVDVDGDGSF 551
           STGVGQHQMW AQFYK+KKPRQWLTSGGLGAMGFGLPAAMGAVVANP AVVVD+DGDGSF
Sbjct: 481 STGVGQHQMWAAQFYKYKKPRQWLTSGGLGAMGFGLPAAMGAVVANPGAVVVDIDGDGSF 540

Query: 552 MMNIQELAAISVEKLPVKILLLNNQYLGMVLQWEDQFNKLGNRAQTYLGDPSKGSEIFPN 611
           +MN+QELA ISVEKLPVKILLLNNQ+LGMV+QWED+F K  NRA TYLG+PS  SEIFPN
Sbjct: 541 IMNVQELATISVEKLPVKILLLNNQHLGMVVQWEDRFYK-ANRAHTYLGNPSNESEIFPN 600

Query: 612 MLKFAEACGIPAARVTKKWEVKTAIKKMLETEGPYLLDVIIPHQEYVLPLIPSGGTFKDV 657
           MLKFAEACGIPAARVTK+ E++ A+KKMLETEGPYLLDVI+PHQE+VLP+IPSGG FKDV
Sbjct: 601 MLKFAEACGIPAARVTKRAELRAAMKKMLETEGPYLLDVIVPHQEHVLPMIPSGGAFKDV 660

[Close]

BLAST of Moc10g25860 vs. ExPASy TrEMBL
Match: A0A1S3CLF6 (Acetolactate synthase OS=Cucumis melo OX=3656 GN=LOC103502317 PE=3 SV=1)

HSP 1 Score: 1037.7 bits (2682), Expect = 2.1e-299
Identity = 538/667 (80.66%), Postives = 588/667 (88.16%), Query Frame = 0


Query: 12  MAAMSFCTS--ISKYSSSSTSFKPSKPISPFFQSFSILTQNHTFFCRPIRICS-SLS-PS 71
           M++M+  TS   SK  S S+SF  +KP S F  SFS L  N T F RP+RICS SLS PS
Sbjct: 1   MSSMAAATSPCASKSFSKSSSFNLTKPFSRFDLSFSSLPHNPT-FRRPLRICSTSLSNPS 60

Query: 72  SVP------------------EIFIVSRFAADEPRKGADILIEALERQGVTSVFAYPGGA 131
             P                  +IF VSRFAADEPRKGADIL+EALERQGVT+VFAYPGGA
Sbjct: 61  PKPSSTAASAAAVETSSIASSDIF-VSRFAADEPRKGADILVEALERQGVTNVFAYPGGA 120

Query: 132 SMEIHQALTRSAIIRDILPRHEQGGVFAAEGYARSSGLPGVCIATSGPGATNLVTGLADA 191
           SMEIHQALTRS+IIR++LPRHEQGGVFAAEGYARSSGLPGVCIATSGPGATNLV+GLADA
Sbjct: 121 SMEIHQALTRSSIIRNVLPRHEQGGVFAAEGYARSSGLPGVCIATSGPGATNLVSGLADA 180

Query: 192 LLDSVPLVAITGQVPRGMMGNNAFQDIPIVEVTRSITKHNYLVLDVDDIPRIVSEAFFLA 251
           LLDSVPLVAITGQVPR M+G +AFQ+ PIVEVTRSITKHNYLVLDVDDIPRIVSEAFFLA
Sbjct: 181 LLDSVPLVAITGQVPRRMIGTDAFQETPIVEVTRSITKHNYLVLDVDDIPRIVSEAFFLA 240

Query: 252 TSGRPGPVLIDIPKDIQQQLAIPNWNQPIKLSAYLSTLPNPPNDAPLDQILRLISESKKP 311
            SGRPGPVLIDIPKD+QQQLA+PNWNQP+KL  YLS LP PP D+ L+QILRLISESKKP
Sbjct: 241 ISGRPGPVLIDIPKDVQQQLAVPNWNQPMKLPGYLSRLPKPPIDSHLEQILRLISESKKP 300

Query: 312 VLYVGGGCLNSPDELRRFVGLTGIPVASTLMGLGAYPCNHELSLQMLGMHGTVYANYAVD 371
           VLYVGGGCLNS +ELRRFV  TGIPVASTLMGLGAYPC  +LSLQMLGMHGTVYANYAVD
Sbjct: 301 VLYVGGGCLNSSEELRRFVKFTGIPVASTLMGLGAYPCADDLSLQMLGMHGTVYANYAVD 360

Query: 372 KSDLLLAFGVRFDERVTGKLEAFASRAKIVHIDIDSYEIGRNKQPHVWVCGDVKLAMQRM 431
           KSDLLLAFGVRFD+RVTGKLEAFASRAKIVHIDIDS EIG+NKQPHV VCGDVKLA+Q M
Sbjct: 361 KSDLLLAFGVRFDDRVTGKLEAFASRAKIVHIDIDSAEIGKNKQPHVSVCGDVKLALQGM 420

Query: 432 NRLMEENAEKLLHCDFSAWREELDGQKSKYPLSYKTFEEAIPPQYAIQVLDELTNGRAII 491
           NRL+E+   ++LH DFSAWREEL+ QKSKYPL++KTF+EAIPPQYAIQ+LDELTNG AII
Sbjct: 421 NRLLEK--AQMLHFDFSAWREELNEQKSKYPLTFKTFDEAIPPQYAIQLLDELTNGEAII 480

Query: 492 STGVGQHQMWVAQFYKFKKPRQWLTSGGLGAMGFGLPAAMGAVVANPEAVVVDVDGDGSF 551
           STGVGQHQMW AQFYK+KKPRQWLTSGGLGAMGFGLPAAMGAVVANP AVVVD+DGDGSF
Sbjct: 481 STGVGQHQMWAAQFYKYKKPRQWLTSGGLGAMGFGLPAAMGAVVANPGAVVVDIDGDGSF 540

Query: 552 MMNIQELAAISVEKLPVKILLLNNQYLGMVLQWEDQFNKLGNRAQTYLGDPSKGSEIFPN 611
           +MN+QELA ISVEKLPVKILLLNNQ+LGMV+QWED+F K  NRA TYLG+PS  SEIFPN
Sbjct: 541 IMNVQELATISVEKLPVKILLLNNQHLGMVVQWEDRFYK-ANRAHTYLGNPSNESEIFPN 600

Query: 612 MLKFAEACGIPAARVTKKWEVKTAIKKMLETEGPYLLDVIIPHQEYVLPLIPSGGTFKDV 657
           MLKFAEACGIPAARVTK+ E++ A+KKMLETEGPYLLDVI+PHQE+VLP+IPSGG FKDV
Sbjct: 601 MLKFAEACGIPAARVTKRAELRAAMKKMLETEGPYLLDVIVPHQEHVLPMIPSGGAFKDV 660

[Close]

BLAST of Moc10g25860 vs. ExPASy TrEMBL
Match: A0A0A0LU17 (Acetolactate synthase OS=Cucumis sativus OX=3659 GN=Csa_1G001520 PE=3 SV=1)

HSP 1 Score: 1035.0 bits (2675), Expect = 1.3e-298
Identity = 537/668 (80.39%), Postives = 585/668 (87.57%), Query Frame = 0


Query: 9   MPSMAAMSFCTSISKYSSSSTSFKPSKPISPFFQSFSILTQNHTFFCRPIRICS-SLS-P 68
           M SMAA +     SK  S S SF  +KP S F  SFS L  N T F R +RICS SLS P
Sbjct: 1   MSSMAAAA-SPCASKSFSKSPSFNLTKPFSRFDLSFSSLPHNPT-FRRSLRICSTSLSNP 60

Query: 69  SSVP------------------EIFIVSRFAADEPRKGADILIEALERQGVTSVFAYPGG 128
           S  P                  EIF  SRFAADEPRKGADIL+EALERQGVT+VFAYPGG
Sbjct: 61  SPKPSSTAASAATVETSSITSSEIF-ASRFAADEPRKGADILVEALERQGVTNVFAYPGG 120

Query: 129 ASMEIHQALTRSAIIRDILPRHEQGGVFAAEGYARSSGLPGVCIATSGPGATNLVTGLAD 188
           ASMEIHQALTRS+IIR++LPRHEQGGVFAAEGYARSSGLPGVCIATSGPGATNLV+GLAD
Sbjct: 121 ASMEIHQALTRSSIIRNVLPRHEQGGVFAAEGYARSSGLPGVCIATSGPGATNLVSGLAD 180

Query: 189 ALLDSVPLVAITGQVPRGMMGNNAFQDIPIVEVTRSITKHNYLVLDVDDIPRIVSEAFFL 248
           ALLDSVPLVAITGQVPR M+G +AFQ+ PIVEVTRSITKHNYLVLDVDDIPRIVSEAFFL
Sbjct: 181 ALLDSVPLVAITGQVPRRMIGTDAFQETPIVEVTRSITKHNYLVLDVDDIPRIVSEAFFL 240

Query: 249 ATSGRPGPVLIDIPKDIQQQLAIPNWNQPIKLSAYLSTLPNPPNDAPLDQILRLISESKK 308
           A+SGRPGPVLIDIPKD+QQQLA+PNWNQP+KL  YLS LP PP D+ L+QILRLISESKK
Sbjct: 241 ASSGRPGPVLIDIPKDVQQQLAVPNWNQPMKLPGYLSRLPKPPTDSHLEQILRLISESKK 300

Query: 309 PVLYVGGGCLNSPDELRRFVGLTGIPVASTLMGLGAYPCNHELSLQMLGMHGTVYANYAV 368
           PVLYVGGGCLNS +ELRRFV LTGIPVASTLMGLGAYPC+ +LSLQMLGMHGTVYANYAV
Sbjct: 301 PVLYVGGGCLNSSEELRRFVKLTGIPVASTLMGLGAYPCSDDLSLQMLGMHGTVYANYAV 360

Query: 369 DKSDLLLAFGVRFDERVTGKLEAFASRAKIVHIDIDSYEIGRNKQPHVWVCGDVKLAMQR 428
           DKSDLLLAFGVRFD+RVTGKLEAFASRAKIVHIDIDS EIG+NKQPHV VCGDVKLA+Q 
Sbjct: 361 DKSDLLLAFGVRFDDRVTGKLEAFASRAKIVHIDIDSAEIGKNKQPHVSVCGDVKLALQG 420

Query: 429 MNRLMEENAEKLLHCDFSAWREELDGQKSKYPLSYKTFEEAIPPQYAIQVLDELTNGRAI 488
           MNRL+E+    +LH DFSAWREEL+ QKSKYPL++KTF+EAIPPQYAIQ+LDELTNG AI
Sbjct: 421 MNRLLEK--AHMLHFDFSAWREELNEQKSKYPLTFKTFDEAIPPQYAIQLLDELTNGEAI 480

Query: 489 ISTGVGQHQMWVAQFYKFKKPRQWLTSGGLGAMGFGLPAAMGAVVANPEAVVVDVDGDGS 548
           +STGVGQHQMW AQFYK+KKPRQWLTSGGLGAMGFGLPAAMGA VANP AVVVD+DGDGS
Sbjct: 481 VSTGVGQHQMWAAQFYKYKKPRQWLTSGGLGAMGFGLPAAMGAAVANPGAVVVDIDGDGS 540

Query: 549 FMMNIQELAAISVEKLPVKILLLNNQYLGMVLQWEDQFNKLGNRAQTYLGDPSKGSEIFP 608
           F+MN+QELA ISVEKLPVKILLLNNQ+LGMV+QWED+F K  NRA TYLG+PS  SEIFP
Sbjct: 541 FIMNVQELATISVEKLPVKILLLNNQHLGMVVQWEDRFYK-ANRAHTYLGNPSNESEIFP 600

Query: 609 NMLKFAEACGIPAARVTKKWEVKTAIKKMLETEGPYLLDVIIPHQEYVLPLIPSGGTFKD 657
           NMLKFAEACGIPAARVTKK E++ A+KKMLETEGPY+LDVI+PHQE+VLP+IPSGG FKD
Sbjct: 601 NMLKFAEACGIPAARVTKKAELRAAMKKMLETEGPYMLDVIVPHQEHVLPMIPSGGAFKD 660

[Close]

BLAST of Moc10g25860 vs. ExPASy TrEMBL
Match: A0A6J1L3F8 (Acetolactate synthase OS=Cucurbita maxima OX=3661 GN=LOC111500182 PE=3 SV=1)

HSP 1 Score: 1031.6 bits (2666), Expect = 1.5e-297
Identity = 532/669 (79.52%), Postives = 585/669 (87.44%), Query Frame = 0


Query: 9   MPSMAAMSFCTSISKYSSSSTSFKPSKPISPFFQSFSILTQNHTFFCRPIRICSSL---- 68
           M SMAA +   +   +S SS+S  P KP S F  SFS L  N TF  RP+R+CSS     
Sbjct: 1   MSSMAAATHPCASKSFSKSSSSVLP-KPFSNFHFSFSPLPHNPTFL-RPLRVCSSSLSNP 60

Query: 69  --SPSSV-------------PEIFIVSRFAADEPRKGADILIEALERQGVTSVFAYPGGA 128
              PSS              PEIF VSRF+ DEPRKGADIL+EALERQGVT+VFAYPGGA
Sbjct: 61  SPKPSSTAASATSVETSVISPEIF-VSRFSPDEPRKGADILVEALERQGVTNVFAYPGGA 120

Query: 129 SMEIHQALTRSAIIRDILPRHEQGGVFAAEGYARSSGLPGVCIATSGPGATNLVTGLADA 188
           SMEIHQALTRS+IIR++LPRHEQGGVFAAEGYARSSGLPGVCIATSGPGATNLV+GLADA
Sbjct: 121 SMEIHQALTRSSIIRNVLPRHEQGGVFAAEGYARSSGLPGVCIATSGPGATNLVSGLADA 180

Query: 189 LLDSVPLVAITGQVPRGMMGNNAFQDIPIVEVTRSITKHNYLVLDVDDIPRIVSEAFFLA 248
           LLDSVP+VAITGQVPR M+G +AFQ+ PIVEVTRSITKHNYLVLDV+DIPRIVSEAFFLA
Sbjct: 181 LLDSVPMVAITGQVPRRMIGTDAFQETPIVEVTRSITKHNYLVLDVEDIPRIVSEAFFLA 240

Query: 249 TSGRPGPVLIDIPKDIQQQLAIPNWNQPIKLSAYLSTLPNPPNDAPLDQILRLISESKKP 308
            SGRPGPVLIDIPKD+QQQLA+PNWNQP+KL  YLS LP PP D  L+QI+RLISESKKP
Sbjct: 241 ISGRPGPVLIDIPKDVQQQLAVPNWNQPMKLPGYLSRLPKPPMDYHLEQIVRLISESKKP 300

Query: 309 VLYVGGGCLNSPDELRRFVGLTGIPVASTLMGLGAYPCNHELSLQMLGMHGTVYANYAVD 368
           VLYVGGGCLNS  ELR+FV LTGIPVASTLMGLGA+PC+ +LSLQMLGMHGTVYANYAVD
Sbjct: 301 VLYVGGGCLNSSVELRQFVKLTGIPVASTLMGLGAFPCSDDLSLQMLGMHGTVYANYAVD 360

Query: 369 KSDLLLAFGVRFDERVTGKLEAFASRAKIVHIDIDSYEIGRNKQPHVWVCGDVKLAMQRM 428
           KSDLLLAFGVRFD+RVTGKLEAFASRAKIVH+DIDS EIG+NKQPHV VC DVKL +QRM
Sbjct: 361 KSDLLLAFGVRFDDRVTGKLEAFASRAKIVHVDIDSAEIGKNKQPHVSVCSDVKLVLQRM 420

Query: 429 NRLMEENAEKLLHCDFSAWREELDGQKSKYPLSYKTFEEAIPPQYAIQVLDELTNGRAII 488
           NRL+ EN+EK LH DFSAWREEL+ QKSKYPL++KTF+EAIPPQYAI VLDELTNG AII
Sbjct: 421 NRLL-ENSEK-LHFDFSAWREELNEQKSKYPLTFKTFDEAIPPQYAILVLDELTNGEAII 480

Query: 489 STGVGQHQMWVAQFYKFKKPRQWLTSGGLGAMGFGLPAAMGAVVANPEAVVVDVDGDGSF 548
           STGVGQHQMW AQFYK+KKPRQWLTSGGLGAMGFGLPAAMGA VANP+AVVVD+DGDGSF
Sbjct: 481 STGVGQHQMWAAQFYKYKKPRQWLTSGGLGAMGFGLPAAMGAAVANPDAVVVDIDGDGSF 540

Query: 549 MMNIQELAAISVEKLPVKILLLNNQYLGMVLQWEDQFNKLGNRAQTYLGDPSKGSEIFPN 608
           MMNIQELA ISVEKLPVKILLLNNQ+LGMV+QWED+F K  NRA TYLGDPS  SEIFPN
Sbjct: 541 MMNIQELATISVEKLPVKILLLNNQHLGMVVQWEDRFYK-ANRAHTYLGDPSNESEIFPN 600

Query: 609 MLKFAEACGIPAARVTKKWEVKTAIKKMLETEGPYLLDVIIPHQEYVLPLIPSGGTFKDV 659
           MLKFAEACG+ AARVTK+ +++ AIKKMLE+EGPYLLDVI+PHQE+VLP+IPSGG FKDV
Sbjct: 601 MLKFAEACGVRAARVTKRADLRAAIKKMLESEGPYLLDVIVPHQEHVLPMIPSGGAFKDV 660

[Close]

BLAST of Moc10g25860 vs. TAIR 10
Match: AT3G48560.1 (chlorsulfuron/imidazolinone resistant 1 )

HSP 1 Score: 956.1 bits (2470), Expect = 1.5e-278
Identity = 491/671 (73.17%), Postives = 556/671 (82.86%), Query Frame = 0


Query: 12  MAAMSFCTSISKYSSSSTSFKPSK-----PISPFFQSFSILTQNHTFFCRPIRICSSLSP 71
           MAA +  T+ S   S ST   PS      PIS F   FS L  N +      R   S SP
Sbjct: 1   MAAATTTTTTSSSISFSTKPSPSSSKSPLPISRFSLPFS-LNPNKSSSSSRRRGIKSSSP 60

Query: 72  SSV---------------------PEIFIVSRFAADEPRKGADILIEALERQGVTSVFAY 131
           SS+                     PE FI SRFA D+PRKGADIL+EALERQGV +VFAY
Sbjct: 61  SSISAVLNTTTNVTTTPSPTKPTKPETFI-SRFAPDQPRKGADILVEALERQGVETVFAY 120

Query: 132 PGGASMEIHQALTRSAIIRDILPRHEQGGVFAAEGYARSSGLPGVCIATSGPGATNLVTG 191
           PGGASMEIHQALTRS+ IR++LPRHEQGGVFAAEGYARSSG PG+CIATSGPGATNLV+G
Sbjct: 121 PGGASMEIHQALTRSSSIRNVLPRHEQGGVFAAEGYARSSGKPGICIATSGPGATNLVSG 180

Query: 192 LADALLDSVPLVAITGQVPRGMMGNNAFQDIPIVEVTRSITKHNYLVLDVDDIPRIVSEA 251
           LADALLDSVPLVAITGQVPR M+G +AFQ+ PIVEVTRSITKHNYLV+DV+DIPRI+ EA
Sbjct: 181 LADALLDSVPLVAITGQVPRRMIGTDAFQETPIVEVTRSITKHNYLVMDVEDIPRIIEEA 240

Query: 252 FFLATSGRPGPVLIDIPKDIQQQLAIPNWNQPIKLSAYLSTLPNPPNDAPLDQILRLISE 311
           FFLATSGRPGPVL+D+PKDIQQQLAIPNW Q ++L  Y+S +P PP D+ L+QI+RLISE
Sbjct: 241 FFLATSGRPGPVLVDVPKDIQQQLAIPNWEQAMRLPGYMSRMPKPPEDSHLEQIVRLISE 300

Query: 312 SKKPVLYVGGGCLNSPDELRRFVGLTGIPVASTLMGLGAYPCNHELSLQMLGMHGTVYAN 371
           SKKPVLYVGGGCLNS DEL RFV LTGIPVASTLMGLG+YPC+ ELSL MLGMHGTVYAN
Sbjct: 301 SKKPVLYVGGGCLNSSDELGRFVELTGIPVASTLMGLGSYPCDDELSLHMLGMHGTVYAN 360

Query: 372 YAVDKSDLLLAFGVRFDERVTGKLEAFASRAKIVHIDIDSYEIGRNKQPHVWVCGDVKLA 431
           YAV+ SDLLLAFGVRFD+RVTGKLEAFASRAKIVHIDIDS EIG+NK PHV VCGDVKLA
Sbjct: 361 YAVEHSDLLLAFGVRFDDRVTGKLEAFASRAKIVHIDIDSAEIGKNKTPHVSVCGDVKLA 420

Query: 432 MQRMNRLMEENAEKLLHCDFSAWREELDGQKSKYPLSYKTFEEAIPPQYAIQVLDELTNG 491
           +Q MN+++E  AE+ L  DF  WR EL+ QK K+PLS+KTF EAIPPQYAI+VLDELT+G
Sbjct: 421 LQGMNKVLENRAEE-LKLDFGVWRNELNVQKQKFPLSFKTFGEAIPPQYAIKVLDELTDG 480

Query: 492 RAIISTGVGQHQMWVAQFYKFKKPRQWLTSGGLGAMGFGLPAAMGAVVANPEAVVVDVDG 551
           +AIISTGVGQHQMW AQFY +KKPRQWL+SGGLGAMGFGLPAA+GA VANP+A+VVD+DG
Sbjct: 481 KAIISTGVGQHQMWAAQFYNYKKPRQWLSSGGLGAMGFGLPAAIGASVANPDAIVVDIDG 540

Query: 552 DGSFMMNIQELAAISVEKLPVKILLLNNQYLGMVLQWEDQFNKLGNRAQTYLGDPSKGSE 611
           DGSF+MN+QELA I VE LPVK+LLLNNQ+LGMV+QWED+F K  NRA T+LGDP++  E
Sbjct: 541 DGSFIMNVQELATIRVENLPVKVLLLNNQHLGMVMQWEDRFYK-ANRAHTFLGDPAQEDE 600

Query: 612 IFPNMLKFAEACGIPAARVTKKWEVKTAIKKMLETEGPYLLDVIIPHQEYVLPLIPSGGT 657
           IFPNML FA ACGIPAARVTKK +++ AI+ ML+T GPYLLDVI PHQE+VLP+IPSGGT
Sbjct: 601 IFPNMLLFAAACGIPAARVTKKADLREAIQTMLDTPGPYLLDVICPHQEHVLPMIPSGGT 660

[Close]

BLAST of Moc10g25860 vs. TAIR 10
Match: AT5G17380.1 (Thiamine pyrophosphate dependent pyruvate decarboxylase family protein )

HSP 1 Score: 173.7 bits (439), Expect = 4.9e-43
Identity = 152/567 (26.81%), Postives = 257/567 (45.33%), Query Frame = 0


Query: 86  GADILIEALERQGVTSVFAYPGGASMEIHQALTRSAI--IRDILPRHEQGGVFAAEGYAR 145
           G  ++ ++L   GVT +F   G   + +    +R+    IR I   +EQ   +AA  Y  
Sbjct: 14  GNVLVAKSLSHLGVTHMF---GVVGIPVTSLASRAMALGIRFIAFHNEQSAGYAASAYGY 73

Query: 146 SSGLPGVCIATSGPGATNLVTGLADALLDSVPLVAITGQVPRGMMGNNAFQDIPIVEVTR 205
            +G PG+ +  SGPG  + + GL++A +++ P+V I+G   +  +G   FQ++  +E  +
Sbjct: 74  LTGKPGILLTVSGPGCVHGLAGLSNAWVNTWPMVMISGSCDQRDVGRGDFQELDQIEAVK 133

Query: 206 SITKHNYLVLDVDDIPRIVSEAFFLATSGRPGPVLIDIPKDIQQQLAIPNWNQPIKLSAY 265
           + +K +    DV +IP  VS     A SGRPG   +DIP D+ +Q    +    +     
Sbjct: 134 AFSKLSEKAKDVREIPDCVSRVLDRAVSGRPGGCYLDIPTDVLRQKISESEADKLVDEVE 193

Query: 266 LSTLPNPPND---APLDQILRLISESKKPVLYVGGGCLNS--PDELRRFVGLTGIPVAST 325
            S    P      + ++  + L+ ++++P++  G G   S   DEL++ V +TGIP   T
Sbjct: 194 RSRKEEPIRGSLRSEIESAVSLLRKAERPLIVFGKGAAYSRAEDELKKLVEITGIPFLPT 253

Query: 326 LMGLGAYPCNHELSLQMLGMHGTVYANYAVDKSDLLLAFGVRFDERV-TGKLEAFASRAK 385
            MG G  P  HE S        T   + A+ K D+ L  G R +  +  G+   +    K
Sbjct: 254 PMGKGLLPDTHEFS-------ATAARSLAIGKCDVALVVGARLNWLLHFGESPKWDKDVK 313

Query: 386 IVHIDIDSYEIGRNKQPHVWVCGDVKLAMQRMNRLMEENAEKLLHCDFSAWREEL----- 445
            + +D+   EI   K PH+ + GD K  +  +NR ++++   L     ++W E +     
Sbjct: 314 FILVDVSEEEIELRK-PHLGIVGDAKTVIGLLNREIKDDPFCL--GKSNSWVESISKKAK 373

Query: 446 -DGQKSKYPLSYKTFE-EAIPPQYAIQVLDELTNGRAIISTGVGQHQMWVAQFYKF-KKP 505
            +G+K +  L+        + P   I+       G + +    G + M V +     K+P
Sbjct: 374 ENGEKMEIQLAKDVVPFNFLTPMRIIRDAILAVEGPSPVVVSEGANTMDVGRSVLVQKEP 433

Query: 506 RQWLTSGGLGAMGFGLPAAMGAVVANPEAVVVDVDGDGSFMMNIQELAAISVEKLPVKIL 565
           R  L +G  G MG GL   + A VA+P+ +VV V+GD  F  +  E+  +    L V I+
Sbjct: 434 RTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYNLAVVII 493

Query: 566 LLNNQYLGMVLQWEDQFNKLGNRAQTYLGDPSK----GSEIFPN--MLKFAEACGIPAAR 625
           + NN   G V   +        R    +  P K     +   PN    K  EA G     
Sbjct: 494 VFNN---GGVYGGD-------RRGPEEISGPHKEDPAPTSFVPNAGYHKLIEAFGGKGYI 553

Query: 626 VTKKWEVKTAIKKMLETEGPYLLDVII 631
           V    E+K+A+ +      P +++VII
Sbjct: 554 VETPDELKSALAESFAARKPAVVNVII 557

[Close]

BLAST of Moc10g25860 vs. TAIR 10
Match: AT5G01330.1 (pyruvate decarboxylase-3 )

HSP 1 Score: 89.7 bits (221), Expect = 9.2e-18
Identity = 122/516 (23.64%), Postives = 211/516 (40.89%), Query Frame = 0


Query: 90  LIEALERQGVTSVFAYPGGASMEIHQALTRSAIIRDILPRHEQGGVFAAEGYARSSGLPG 149
           L   L + GVT +F  PG  ++ +   L  +  + +I   +E    +AA+GYARS G+ G
Sbjct: 36  LSRRLVQAGVTDIFTVPGDFNLSLLDQLIANPELNNIGCCNELNAGYAADGYARSRGV-G 95

Query: 150 VCIATSGPGATNLVTGLADALLDSVPLVAITGQVPRGMMGNNAFQDIPI--------VEV 209
            C+ T   G  +++  +A A  +++P++ I G       G N      I        +  
Sbjct: 96  ACVVTFTVGGLSVLNAIAGAYSENLPVICIVGGPNSNDFGTNRILHHTIGLPDFSQELRC 155

Query: 210 TRSITKHNYLVLDVDDIPRIVSEAFFLATSGRPG-PVLIDIPKDIQQQLAIP-----NWN 269
            +++T +  +V  ++D    + +A  +AT+ R   PV I I  ++    AIP     ++ 
Sbjct: 156 FQTVTCYQAVVNHLEDAHEQIDKA--IATALRESKPVYISISCNL---AAIPHPTFASYP 215

Query: 270 QPIKLSAYLSTLPNPPNDAPLDQILRLISESKKPVLYVGGGCLNSPDELRRFVGL---TG 329
            P  L+  LS       +A ++  L  ++++ KPV+ VGG  L        FV L   +G
Sbjct: 216 VPFDLTPRLSN--KDCLEAAVEATLEFLNKAVKPVM-VGGPKLRVAKARDAFVELADASG 275

Query: 330 IPVASTLMGLGAYPCNHELSL-QMLGMHGTVYANYAVDKSDLLLAFGVRFDERVTGKLEA 389
            PVA      G  P NH   +    G   T++ +  V+ +D  +  G  F++  +     
Sbjct: 276 YPVAVMPSAKGFVPENHPHFIGTYWGAVSTLFCSEIVESADAYIFAGPIFNDYSSVGYSL 335

Query: 390 FASRAKIVHIDIDSYEIGRNKQPHVWVCGDVKLAMQRMNRLMEENAEKLLHCDFSAWREE 449
              + K + +  DS          V V         RM+    E A+++     +     
Sbjct: 336 LLKKEKAIIVHPDS----------VVVANGPTFGCVRMSEFFRELAKRVKPNKTAYENYH 395

Query: 450 LDGQKSKYPLSYKTFEEAIPPQYAIQVLDELTNGRAIIS-TGVGQHQMWVAQFYKFKKPR 509
                   PL  K  E          +   L+N  A+I+ TG      +  Q  K  K  
Sbjct: 396 RIFVPEGKPLKCKPREPLRINAMFQHIQKMLSNETAVIAETG---DSWFNCQKLKLPKGC 455

Query: 510 QWLTSGGLGAMGFGLPAAMGAVVANPEAVVVDVDGDGSFMMNIQELAAISVEKLPVKILL 569
            +      G++G+ + A +G   A PE  V+   GDGSF +  Q+++ +        I L
Sbjct: 456 GYEFQMQYGSIGWSVGATLGYAQATPEKRVLSFIGDGSFQVTAQDVSTMIRNGQKTIIFL 515

Query: 570 LNNQYLGMVLQWED-QFNKLGNRAQTYLGDPSKGSE 586
           +NN    + ++  D  +N + N   T L D     E
Sbjct: 516 INNGGYTIEVEIHDGPYNVIKNWNYTGLVDAIHNGE 529

[Close]

BLAST of Moc10g25860 vs. TAIR 10
Match: AT4G33070.1 (Thiamine pyrophosphate dependent pyruvate decarboxylase family protein )

HSP 1 Score: 83.6 bits (205), Expect = 6.6e-16
Identity = 116/513 (22.61%), Postives = 206/513 (40.16%), Query Frame = 0


Query: 90  LIEALERQGVTSVFAYPGGASMEIHQALTRSAIIRDILPRHEQGGVFAAEGYARSSGLPG 149
           L   L + GVT VF+ PG  ++ +   L     +  I   +E    +AA+GYARS G+ G
Sbjct: 51  LARRLVQAGVTDVFSVPGDFNLTLLDHLMAEPDLNLIGCCNELNAGYAADGYARSRGV-G 110

Query: 150 VCIATSGPGATNLVTGLADALLDSVPLVAITGQVPRGMMGNNAFQDIPI--------VEV 209
            C+ T   G  +++  +A A  +++PL+ I G       G N      I        +  
Sbjct: 111 ACVVTFTVGGLSVLNAIAGAYSENLPLICIVGGPNSNDYGTNRILHHTIGLPDFSQELRC 170

Query: 210 TRSITKHNYLVLDVDDIPRIVSEAFFLATSGRPGPVLIDIPKDIQQQLAIPNW---NQPI 269
            +++T +  +V ++DD    + +A   A      PV I +  ++    AIP+      P+
Sbjct: 171 FQTVTCYQAVVNNLDDAHEQIDKAISTALK-ESKPVYISVSCNL---AAIPHHTFSRDPV 230

Query: 270 KLSAYLSTLPNPPNDAPLDQILRLISESKKPVLYVGGGCLNSPDELRRFVGL---TGIPV 329
             S           +A ++  L  ++++ KPV+ VGG  L        FV L   +G  +
Sbjct: 231 PFSLAPRLSNKMGLEAAVEATLEFLNKAVKPVM-VGGPKLRVAKACDAFVELADASGYAL 290

Query: 330 ASTLMGLGAYPCNHELSL-QMLGMHGTVYANYAVDKSDLLLAFGVRFDERVTGKLEAFAS 389
           A      G  P +H   +    G   T + +  V+ +D  +  G  F++  +        
Sbjct: 291 AMMPSAKGFVPEHHPHFIGTYWGAVSTPFCSEIVESADAYIFAGPIFNDYSSVGYSLLLK 350

Query: 390 RAKIVHIDIDSYEIGRNKQPHVWVCGD-VKLAMQRMNRLMEENAEKLLHCDFSAWREELD 449
           + K + +  D   +         +  D  +   +R+ R   E A +  H  F    + L 
Sbjct: 351 KEKAIVVQPDRITVANGPTFGCILMSDFFRELSKRVKR--NETAYENYHRIFVPEGKPLK 410

Query: 450 GQKSKYPLSYKTFEEAIPPQYAIQVLDELTNGRAIISTGVGQHQMWVAQFYKFKKPRQWL 509
            + S+ PL   T  + I    + +          I  TG      +  Q  K  K   + 
Sbjct: 411 CE-SREPLRVNTMFQHIQKMLSSET-------AVIAETG---DSWFNCQKLKLPKGCGYE 470

Query: 510 TSGGLGAMGFGLPAAMGAVVANPEAVVVDVDGDGSFMMNIQELAAISVEKLPVKILLLNN 569
                G++G+ + A +G   A+PE  V+   GDGSF + +Q+++ +        I L+NN
Sbjct: 471 FQMQYGSIGWSVGATLGYAQASPEKRVLAFIGDGSFQVTVQDISTMLRNGQKTIIFLINN 530

Query: 570 QYLGMVLQWED-QFNKLGNRAQTYLGDPSKGSE 586
               + ++  D  +N + N   T L D     E
Sbjct: 531 GGYTIEVEIHDGPYNVIKNWNYTGLVDAIHNGE 544

[Close]

BLAST of Moc10g25860 vs. TAIR 10
Match: AT5G01320.1 (Thiamine pyrophosphate dependent pyruvate decarboxylase family protein )

HSP 1 Score: 82.0 bits (201), Expect = 1.9e-15
Identity = 113/511 (22.11%), Postives = 199/511 (38.94%), Query Frame = 0


Query: 90  LIEALERQGVTSVFAYPGGASMEIHQALTRSAIIRDILPRHEQGGVFAAEGYARSSGLPG 149
           L   L + GVT VF+ PG  ++ +   L     + +I   +E    +AA+GYARS G+ G
Sbjct: 47  LSRRLVQAGVTDVFSVPGDFNLTLLDHLIAEPELNNIGCCNELNAGYAADGYARSRGV-G 106

Query: 150 VCIATSGPGATNLVTGLADALLDSVPLVAITGQVPRGMMGNNAFQDIPI--------VEV 209
            C+ T   G  +++  +A A  +++P++ I G       G N      I        +  
Sbjct: 107 ACVVTFTVGGLSVLNAIAGAYSENLPVICIVGGPNSNDFGTNRILHHTIGLPDFSQELRC 166

Query: 210 TRSITKHNYLVLDVDDIPRIVSEAFFLATSGRPGPVLIDIPKDIQQQLAIPNWNQPIKLS 269
            +++T +  +V +++D    + +A   A      PV I I  ++           P+   
Sbjct: 167 FQTVTCYQAVVNNLEDAHEQIDKAIATALK-ESKPVYISISCNLAATPHPTFARDPVPFD 226

Query: 270 AYLSTLPNPPNDAPLDQILRLISESKKPVLYVGGGCLNSPDELRRFVGL---TGIPVAST 329
                      +A ++  L  ++++ KPV+ VGG  L        F+ L   +G P+A  
Sbjct: 227 LTPRMSNTMGLEAAVEATLEFLNKAVKPVM-VGGPKLRVAKASEAFLELADASGYPLAVM 286

Query: 330 LMGLGAYPCNHELSL-QMLGMHGTVYANYAVDKSDLLLAFGVRFDE--RVTGKLEAFASR 389
               G  P NH   +    G   T + +  V+ +D  +  G  F++   V   L     +
Sbjct: 287 PSTKGLVPENHPHFIGTYWGAVSTPFCSEIVESADAYIFAGPIFNDYSSVGYSLLLKKEK 346

Query: 390 AKIVHIDIDSYEIGRNKQPHVWVCGDVKLAMQRMNRLMEENAEKLLHCDFSAWREELDGQ 449
           A IVH D             V V          M+    E A+++   + +    E    
Sbjct: 347 AIIVHPD------------RVVVANGPTFGCVLMSDFFRELAKRVKRNETAYENYERIFV 406

Query: 450 KSKYPLSYKTFEEAIPPQYAIQVLDELTNGRAIISTGVGQHQMWVAQFYKFKKPRQWLTS 509
               PL  K   E +      Q + ++ +    +    G    +  Q  K  K   +   
Sbjct: 407 PEGKPLKCKP-GEPLRVNAMFQHIQKMLSSETAVIAETGD-SWFNCQKLKLPKGCGYEFQ 466

Query: 510 GGLGAMGFGLPAAMGAVVANPEAVVVDVDGDGSFMMNIQELAAISVEKLPVKILLLNNQY 569
              G++G+ + A +G   A PE  V+   GDGSF +  Q+++ +        I L+NN  
Sbjct: 467 MQYGSIGWSVGATLGYAQATPEKRVLSFIGDGSFQVTAQDISTMIRNGQKAIIFLINNGG 526

Query: 570 LGMVLQWED-QFNKLGNRAQTYLGDPSKGSE 586
             + ++  D  +N + N   T L D     E
Sbjct: 527 YTIEVEIHDGPYNVIKNWNYTGLVDAIHNGE 540

The following BLAST results are available for this feature:

BLAST of Moc10g25860 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143464.1	0.0e+00	100.00	acetolactate synthase 3, chloroplastic-like [Momordica charantia]	[more]
XP_008464426.1	4.2e-299	80.66	PREDICTED: acetolactate synthase 3, chloroplastic [Cucumis melo] >KAA0057770.1 a...	[more]
XP_038878927.1	9.5e-299	79.14	acetolactate synthase 3, chloroplastic [Benincasa hispida]	[more]
XP_004138045.1	2.7e-298	80.39	acetolactate synthase 3, chloroplastic [Cucumis sativus] >KGN63481.1 hypothetica...	[more]
XP_023007605.1	3.0e-297	79.52	acetolactate synthase 2, chloroplastic-like [Cucurbita maxima]	[more]

BLAST of Moc10g25860 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P27819	1.1e-278	75.19	Acetolactate synthase 3, chloroplastic OS=Brassica napus OX=3708 PE=3 SV=1	[more]
P27818	1.9e-278	74.85	Acetolactate synthase 1, chloroplastic OS=Brassica napus OX=3708 PE=3 SV=1	[more]
P17597	2.1e-277	73.17	Acetolactate synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ALS PE=1...	[more]
P09114	1.4e-273	77.00	Acetolactate synthase 2, chloroplastic OS=Nicotiana tabacum OX=4097 GN=ALS SURB ...	[more]
P09342	1.0e-271	71.04	Acetolactate synthase 1, chloroplastic OS=Nicotiana tabacum OX=4097 GN=ALS SURA ...	[more]

BLAST of Moc10g25860 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CPD6	0.0e+00	100.00	Acetolactate synthase OS=Momordica charantia OX=3673 GN=LOC111013340 PE=3 SV=1	[more]
A0A5A7URM3	2.1e-299	80.66	Acetolactate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35...	[more]
A0A1S3CLF6	2.1e-299	80.66	Acetolactate synthase OS=Cucumis melo OX=3656 GN=LOC103502317 PE=3 SV=1	[more]
A0A0A0LU17	1.3e-298	80.39	Acetolactate synthase OS=Cucumis sativus OX=3659 GN=Csa_1G001520 PE=3 SV=1	[more]
A0A6J1L3F8	1.5e-297	79.52	Acetolactate synthase OS=Cucurbita maxima OX=3661 GN=LOC111500182 PE=3 SV=1	[more]

BLAST of Moc10g25860 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G48560.1	1.5e-278	73.17	chlorsulfuron/imidazolinone resistant 1	[more]
AT5G17380.1	4.9e-43	26.81	Thiamine pyrophosphate dependent pyruvate decarboxylase family protein	[more]
AT5G01330.1	9.2e-18	23.64	pyruvate decarboxylase-3	[more]
AT4G33070.1	6.6e-16	22.61	Thiamine pyrophosphate dependent pyruvate decarboxylase family protein	[more]
AT5G01320.1	1.9e-15	22.11	Thiamine pyrophosphate dependent pyruvate decarboxylase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012001	Thiamine pyrophosphate enzyme, N-terminal TPP-binding domain	PFAM	PF02776	TPP_enzyme_N	coord: 85..248 e-value: 8.7E-50 score: 168.7
IPR012846	Acetolactate synthase, large subunit, biosynthetic	TIGRFAM	TIGR00118	TIGR00118	coord: 85..651 e-value: 1.1E-199 score: 662.5
None	No IPR available	GENE3D	3.40.50.970		coord: 73..267 e-value: 2.0E-65 score: 221.7
None	No IPR available	GENE3D	3.40.50.970		coord: 442..629 e-value: 3.8E-139 score: 465.3
None	No IPR available	GENE3D	3.40.50.1220		coord: 276..441 e-value: 3.8E-139 score: 465.3
None	No IPR available	PIRSR	PIRSR036565-2	PIRSR036565-2	coord: 88..554 e-value: 4.0E-35 score: 119.0
None	No IPR available	PANTHER	PTHR18968	THIAMINE PYROPHOSPHATE ENZYMES	coord: 59..658
None	No IPR available	PANTHER	PTHR18968:SF161	ACETOLACTATE SYNTHASE	coord: 59..658
None	No IPR available	CDD	cd07035	TPP_PYR_POX_like	coord: 88..243 e-value: 1.03336E-68 score: 218.94
IPR012000	Thiamine pyrophosphate enzyme, central domain	PFAM	PF00205	TPP_enzyme_M	coord: 277..408 e-value: 4.8E-42 score: 143.0
IPR011766	Thiamine pyrophosphate enzyme, C-terminal TPP-binding	PFAM	PF02775	TPP_enzyme_C	coord: 472..628 e-value: 4.0E-44 score: 150.1
IPR000399	TPP-binding enzyme, conserved site	PROSITE	PS00187	TPP_ENZYMES	coord: 509..528
IPR039368	Acetolactate synthase large subunit, TPP binding domain	CDD	cd02015	TPP_AHAS	coord: 450..643 e-value: 2.28165E-105 score: 315.592
IPR029061	Thiamin diphosphate-binding fold	SUPERFAMILY	52518	Thiamin diphosphate-binding fold (THDP-binding)	coord: 450..650
IPR029061	Thiamin diphosphate-binding fold	SUPERFAMILY	52518	Thiamin diphosphate-binding fold (THDP-binding)	coord: 81..255
IPR029035	DHS-like NAD/FAD-binding domain superfamily	SUPERFAMILY	52467	DHS-like NAD/FAD-binding domain	coord: 272..441

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc10g25860.1	Moc10g25860.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009097	isoleucine biosynthetic process
biological_process	GO:0009635	response to herbicide
biological_process	GO:0009099	valine biosynthetic process
biological_process	GO:0009082	branched-chain amino acid biosynthetic process
cellular_component	GO:0005948	acetolactate synthase complex
molecular_function	GO:0003984	acetolactate synthase activity
molecular_function	GO:0050660	flavin adenine dinucleotide binding
molecular_function	GO:0000287	magnesium ion binding
molecular_function	GO:0030976	thiamine pyrophosphate binding
molecular_function	GO:0003824	catalytic activity