Moc10g23970 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc10g23970
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionReverse transcriptase
Locationchr10: 17207893 .. 17208620 (-)
RNA-Seq ExpressionMoc10g23970
SyntenyMoc10g23970
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTAAGATAGAAAGATTTAACAGCATGCTCAAGGAATACTTGCGTCACTTTGTCTATTCCAAACAAAGAAATTGGGTCTAGTTGTTAGATGTGGCTCAATTTTGCTTTAACAGCCAGACAAGCTCATCAACAGGGAAGAGCCCCTTCGAGATTGTATGTGGCAGACAATCGTCTATGCCACATATCATTAACTACCCATACGCTGGGAAGAGTCCTCAAGCCCACAATCTCCCAAAGGAATGGAAGCAAATGATAGAGATTGCTTGAGCGTATTTGGAAAACGCTTACAAGCACGTGAAGAAATGGGCAGATCGCAAGCGTCGCCCCCTTGAATTTTAAGCGGATGACAAAGTTCTCATCAAGTTGAGGCCAGAATAGATTCATTTATGGAGACGCAAAGATCAAAGACTCGTGAGAAAATAAGAAGGACCCGTGGAAGTTTTGAAGAAGGCAGGGAATGTCTCGTATAAGGTGTTGTTACCAGCCTGGATGAAGATCCACCCGGTAATCCATGTGAGCAACTTAAAACCTTATCACCTCGATCCTAAGGATGACGAACACAATGTTGTAGTGTGGCCGGCCATCGACCTAAAGCTTAAGGACGACAAGGAAGCAGAAGAGATCCTTGCAGAGAGAGTCAGAAAGGTGGGAAGACCTGTTCGCAAAGTTCAAGAATTCCTCATCAAATGGAAGGACTTACCTTATGCAGAAATCAGCTGGGAGTGA

mRNA sequence

ATGCCAGACAAGCTCATCAACAGGGAAGAGCCCCTTCGAGATTCCTGGATGAAGATCCACCCGGTAATCCATGTGAGCAACTTAAAACCTTATCACCTCGATCCTAAGGATGACGAACACAATGTTGTAGTGTGGCCGGCCATCGACCTAAAGCTTAAGGACGACAAGGAAGCAGAAGAGATCCTTGCAGAGAGAGTCAGAAAGGTGGGAAGACCTGTTCGCAAAGTTCAAGAATTCCTCATCAAATGGAAGGACTTACCTTATGCAGAAATCAGCTGGGAGTGA

Coding sequence (CDS)

ATGCCAGACAAGCTCATCAACAGGGAAGAGCCCCTTCGAGATTCCTGGATGAAGATCCACCCGGTAATCCATGTGAGCAACTTAAAACCTTATCACCTCGATCCTAAGGATGACGAACACAATGTTGTAGTGTGGCCGGCCATCGACCTAAAGCTTAAGGACGACAAGGAAGCAGAAGAGATCCTTGCAGAGAGAGTCAGAAAGGTGGGAAGACCTGTTCGCAAAGTTCAAGAATTCCTCATCAAATGGAAGGACTTACCTTATGCAGAAATCAGCTGGGAGTGA

Protein sequence

MPDKLINREEPLRDSWMKIHPVIHVSNLKPYHLDPKDDEHNVVVWPAIDLKLKDDKEAEEILAERVRKVGRPVRKVQEFLIKWKDLPYAEISWE
Homology
BLAST of Moc10g23970 vs. NCBI nr
Match: KAA0053507.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 114.4 bits (285), Expect = 5.4e-22
Identity = 51/80 (63.75%), Postives = 64/80 (80.00%), Query Frame = 0

Query: 15  SWMKIHPVIHVSNLKPYHLDPKDDEHNVVVWPAIDLKLKDDKEAEEILAERVRKVGRPVR 74
           +WMKI+PVIHVSNLKPYH D +D + N+V  P IDL  K+DK+ EEILAERVR+  RP R
Sbjct: 511 TWMKIYPVIHVSNLKPYHQDTEDLQRNIVTRPVIDLSQKEDKDVEEILAERVRRGRRPAR 570

Query: 75  KVQEFLIKWKDLPYAEISWE 95
           ++ E+L+KWK+LP  EISWE
Sbjct: 571 RIHEYLVKWKNLPVEEISWE 590

BLAST of Moc10g23970 vs. NCBI nr
Match: KAA0032849.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 114.0 bits (284), Expect = 7.0e-22
Identity = 52/80 (65.00%), Postives = 63/80 (78.75%), Query Frame = 0

Query: 15   SWMKIHPVIHVSNLKPYHLDPKDDEHNVVVWPAIDLKLKDDKEAEEILAERVRKVGRPVR 74
            +WMKI+PVIHVSNLKPYH D +D + NVV  P IDL  K+DK+ EEILAERVRK  RP R
Sbjct: 1482 TWMKIYPVIHVSNLKPYHQDTEDLQRNVVTRPIIDLSQKEDKDVEEILAERVRKSRRPAR 1541

Query: 75   KVQEFLIKWKDLPYAEISWE 95
            ++ E+L+KWK+LP  E SWE
Sbjct: 1542 RIHEYLVKWKNLPVEETSWE 1561

BLAST of Moc10g23970 vs. NCBI nr
Match: KAA0032476.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 112.8 bits (281), Expect = 1.6e-21
Identity = 51/80 (63.75%), Postives = 63/80 (78.75%), Query Frame = 0

Query: 15  SWMKIHPVIHVSNLKPYHLDPKDDEHNVVVWPAIDLKLKDDKEAEEILAERVRKVGRPVR 74
           +WMKI+PVIHVSNLKPYH D +D + NVV  P IDL  K+DK+ EEILAERVR+  RP R
Sbjct: 64  TWMKIYPVIHVSNLKPYHQDTEDLQRNVVTRPTIDLSQKEDKDVEEILAERVRRGRRPAR 123

Query: 75  KVQEFLIKWKDLPYAEISWE 95
           ++ E+L+KWK+LP  E SWE
Sbjct: 124 RIHEYLVKWKNLPVEETSWE 143

BLAST of Moc10g23970 vs. NCBI nr
Match: KAA0067557.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 112.8 bits (281), Expect = 1.6e-21
Identity = 51/80 (63.75%), Postives = 63/80 (78.75%), Query Frame = 0

Query: 15   SWMKIHPVIHVSNLKPYHLDPKDDEHNVVVWPAIDLKLKDDKEAEEILAERVRKVGRPVR 74
            +WMKI+PVIHVSNLKPYH D +D + NVV  P IDL  K+DK+ EEILAERVR+  RP R
Sbjct: 1478 TWMKIYPVIHVSNLKPYHQDTEDLQRNVVTRPTIDLSQKEDKDVEEILAERVRRGRRPAR 1537

Query: 75   KVQEFLIKWKDLPYAEISWE 95
            ++ E+L+KWK+LP  E SWE
Sbjct: 1538 RIHEYLVKWKNLPVEETSWE 1557

BLAST of Moc10g23970 vs. NCBI nr
Match: TYK19637.1 (reverse transcriptase [Cucumis melo var. makuwa] >TYK20902.1 reverse transcriptase [Cucumis melo var. makuwa] >TYK28777.1 reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 112.8 bits (281), Expect = 1.6e-21
Identity = 51/80 (63.75%), Postives = 63/80 (78.75%), Query Frame = 0

Query: 15  SWMKIHPVIHVSNLKPYHLDPKDDEHNVVVWPAIDLKLKDDKEAEEILAERVRKVGRPVR 74
           +WMKI+PVIHVSNLKPYH D +D + NVV  P IDL  K+DK+ EEILAERVR+  RP R
Sbjct: 154 TWMKIYPVIHVSNLKPYHQDTEDLQRNVVTRPTIDLSQKEDKDVEEILAERVRRGRRPAR 213

Query: 75  KVQEFLIKWKDLPYAEISWE 95
           ++ E+L+KWK+LP  E SWE
Sbjct: 214 RIHEYLVKWKNLPVEETSWE 233

BLAST of Moc10g23970 vs. ExPASy TrEMBL
Match: A0A5A7UG93 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold190G00640 PE=4 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 2.6e-22
Identity = 51/80 (63.75%), Postives = 64/80 (80.00%), Query Frame = 0

Query: 15  SWMKIHPVIHVSNLKPYHLDPKDDEHNVVVWPAIDLKLKDDKEAEEILAERVRKVGRPVR 74
           +WMKI+PVIHVSNLKPYH D +D + N+V  P IDL  K+DK+ EEILAERVR+  RP R
Sbjct: 511 TWMKIYPVIHVSNLKPYHQDTEDLQRNIVTRPVIDLSQKEDKDVEEILAERVRRGRRPAR 570

Query: 75  KVQEFLIKWKDLPYAEISWE 95
           ++ E+L+KWK+LP  EISWE
Sbjct: 571 RIHEYLVKWKNLPVEEISWE 590

BLAST of Moc10g23970 vs. ExPASy TrEMBL
Match: A0A5A7SUK4 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold81G00100 PE=4 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 3.4e-22
Identity = 52/80 (65.00%), Postives = 63/80 (78.75%), Query Frame = 0

Query: 15   SWMKIHPVIHVSNLKPYHLDPKDDEHNVVVWPAIDLKLKDDKEAEEILAERVRKVGRPVR 74
            +WMKI+PVIHVSNLKPYH D +D + NVV  P IDL  K+DK+ EEILAERVRK  RP R
Sbjct: 1482 TWMKIYPVIHVSNLKPYHQDTEDLQRNVVTRPIIDLSQKEDKDVEEILAERVRKSRRPAR 1541

Query: 75   KVQEFLIKWKDLPYAEISWE 95
            ++ E+L+KWK+LP  E SWE
Sbjct: 1542 RIHEYLVKWKNLPVEETSWE 1561

BLAST of Moc10g23970 vs. ExPASy TrEMBL
Match: A0A5D3C4R1 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold110G001990 PE=4 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 7.6e-22
Identity = 51/80 (63.75%), Postives = 63/80 (78.75%), Query Frame = 0

Query: 15   SWMKIHPVIHVSNLKPYHLDPKDDEHNVVVWPAIDLKLKDDKEAEEILAERVRKVGRPVR 74
            +WMKI+PVIHVSNLKPYH D +D + NVV  P IDL  K+DK+ EEILAERVR+  RP R
Sbjct: 1482 TWMKIYPVIHVSNLKPYHQDTEDLQRNVVTRPTIDLSQKEDKDVEEILAERVRRGRRPAR 1541

Query: 75   KVQEFLIKWKDLPYAEISWE 95
            ++ E+L+KWK+LP  E SWE
Sbjct: 1542 RIHEYLVKWKNLPVEETSWE 1561

BLAST of Moc10g23970 vs. ExPASy TrEMBL
Match: A0A5D3DBE0 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold242G00240 PE=4 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 7.6e-22
Identity = 51/80 (63.75%), Postives = 63/80 (78.75%), Query Frame = 0

Query: 15  SWMKIHPVIHVSNLKPYHLDPKDDEHNVVVWPAIDLKLKDDKEAEEILAERVRKVGRPVR 74
           +WMKI+PVIHVSNLKPYH D +D + NVV  P IDL  K+DK+ EEILAERVR+  RP R
Sbjct: 154 TWMKIYPVIHVSNLKPYHQDTEDLQRNVVTRPTIDLSQKEDKDVEEILAERVRRGRRPAR 213

Query: 75  KVQEFLIKWKDLPYAEISWE 95
           ++ E+L+KWK+LP  E SWE
Sbjct: 214 RIHEYLVKWKNLPVEETSWE 233

BLAST of Moc10g23970 vs. ExPASy TrEMBL
Match: A0A5A7UXR6 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold22G005550 PE=4 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 7.6e-22
Identity = 51/80 (63.75%), Postives = 63/80 (78.75%), Query Frame = 0

Query: 15   SWMKIHPVIHVSNLKPYHLDPKDDEHNVVVWPAIDLKLKDDKEAEEILAERVRKVGRPVR 74
            +WMKI+PVIHVSNLKPYH D +D + NVV  P IDL  K+DK+ EEILAERVR+  RP R
Sbjct: 1482 TWMKIYPVIHVSNLKPYHQDTEDLQRNVVTRPTIDLSQKEDKDVEEILAERVRRGRRPAR 1541

Query: 75   KVQEFLIKWKDLPYAEISWE 95
            ++ E+L+KWK+LP  E SWE
Sbjct: 1542 RIHEYLVKWKNLPVEETSWE 1561

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0053507.15.4e-2263.75reverse transcriptase [Cucumis melo var. makuwa][more]
KAA0032849.17.0e-2265.00reverse transcriptase [Cucumis melo var. makuwa][more]
KAA0032476.11.6e-2163.75reverse transcriptase [Cucumis melo var. makuwa][more]
KAA0067557.11.6e-2163.75reverse transcriptase [Cucumis melo var. makuwa][more]
TYK19637.11.6e-2163.75reverse transcriptase [Cucumis melo var. makuwa] >TYK20902.1 reverse transcripta... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5A7UG932.6e-2263.75Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold19... [more]
A0A5A7SUK43.4e-2265.00Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold81... [more]
A0A5D3C4R17.6e-2263.75Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold11... [more]
A0A5D3DBE07.6e-2263.75Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold24... [more]
A0A5A7UXR67.6e-2263.75Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold22... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023780Chromo domainPFAMPF00385Chromocoord: 57..94
e-value: 4.9E-8
score: 32.8
NoneNo IPR availableGENE3D2.40.50.40coord: 6..94
e-value: 4.7E-8
score: 34.7
IPR000953Chromo/chromo shadow domainPROSITEPS50013CHROMO_2coord: 56..94
score: 11.580101
IPR016197Chromo-like domain superfamilySUPERFAMILY54160Chromo domain-likecoord: 17..94

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc10g23970.1Moc10g23970.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015074 DNA integration
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
biological_process GO:0006508 proteolysis
biological_process GO:0006278 RNA-dependent DNA biosynthetic process
molecular_function GO:0004190 aspartic-type endopeptidase activity
molecular_function GO:0004519 endonuclease activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0003964 RNA-directed DNA polymerase activity
molecular_function GO:0008270 zinc ion binding