Moc10g15230 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc10g15230
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptioncytochrome P450 CYP736A12-like
Locationchr10: 11537398 .. 11538943 (-)
RNA-Seq ExpressionMoc10g15230
SyntenyMoc10g15230
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTGGATTTCAGCCACCATTATACTAACCCTCTTTCTTCTTGCTTGTCTCTTTCAGCCATGGCTGCCCAAGAAGAAGCTTCCTCCTGGCCCAAAAGGGCTTCCCATCATTGGCAGTCTCCATCTTTTAGGAAAACTTGTTCACAGAGATCTCCATAACCTCTCCCAAAGATACGGCCCCATAATGCACGTCCGGTTCGGGCTCGTCCCCGCCATCATCGTGTCTTCCCCTCACGCTTGCAAGCTCTTCCTCAAAACCCAGGACCCTGCATTTGCAGGTCGCCCACTGCCTCACACTTCCAACCAAATGTCCTACGGCGGAAAAGATATCGTGTTTCATGAATACGATTCTTATTGGCGCAGTATGCGAAAAATGTGCTCTTTTGAGCTGCTCAACACTCCTAAAATCAACTCCTTTAGATCCATGAGAAAGCAAGAGATCGGTTTTTTGGTCGATCGTCTCCGAGAGGCGGCGGGAAATCGTGTGGCGGTGGATCTTAACAATGAAGCTTCGTCTCTGATTATGGATGTAGTTTGTTTGATGGTGTTTGGGAAGAATTGTGGGGATGAGGAGTTTAAGAGTGGGATTCATGAGGCGTCGCGGTTGGCGGGAGCTCCTAACTTGGGGGATTTTATTCCTTTTCTTGCTTTGCTTGATTTGCAAGGATTAGGGCGTCGTGCGAAATGTGTTACTAAGGTGTTTGACGGGGTTCTTGGAGAGGATTATCGAGGAACATGTTGAGTTCAAAGATGGAGATAAAACCAAGGATTTTGTGGATGTTATGTTGGAACTTATGAAACAGAGAGACCGAGTTGAGTACCAATTTGATCGCTCCAGCATTAAAGCTATAATTCTCGTAAGTAAACTTTACAGCGAGAATCGTACCCAGTTCTTTTTTTTTTTAGTTAAATATATGGGAGTGGGGATTCGATTTAGCTATGCTCGGGTTGACACCATTACCCAAACACGATTTTTACTTTAAAAGGATGTAAAAACAAAAGTGGCCTGTTTTTGCAAAATTTTATAGACAAAAATGGTCATATTCGACCAGGTTTTATAGACTCTTCATTTAAATTACCAATTTAACCTTATCCTATATTTTTTTTTATTTATAAATCAAAATTGAAAGGTTATATTATATTAGTTAACTTTTTTTTTTCCTCTGCTAGCGAACGTGTACAAACAAAAAAAATTCAGTTACTTTCATTTAAAATAATCTTTTATTATTCGGAACGAAATAAATAATTTAAAATCTTTTTCAATATTTTAAAATAAAAGATTTAAAATTATTTCTTAAACATTAAATTTTACAACTTGAAAGTGATTTTTGATGTATTTAATCAACAACCATAATTTGCAGGATTTGATTATTGGAGCAGTGGACAGTTCAAGCACTGCAATAAATTGGGCATTACCAGAGCTCATCAAGCATCAACACATAATGAAGAAAGTGCAGGTGGAGTTAGAAAATGTGGTTGGTTTGGATAGGATGGTAGAAGAATCCGACTTGAGTCGCTTTGAAATATCTGGAGATGGTCATTAA

mRNA sequence

ATGGCTTGGATTTCAGCCACCATTATACTAACCCTCTTTCTTCTTGCTTGTCTCTTTCAGCCATGGCTGCCCAAGAAGAAGCTTCCTCCTGGCCCAAAAGGGCTTCCCATCATTGGCAGTCTCCATCTTTTAGGAAAACTTGTTCACAGAGATCTCCATAACCTCTCCCAAAGATACGGCCCCATAATGCACGTCCGGTTCGGGCTCGTCCCCGCCATCATCGTGTCTTCCCCTCACGCTTGCAAGCTCTTCCTCAAAACCCAGGACCCTGCATTTGCAGGTCGCCCACTGCCTCACACTTCCAACCAAATGTCCTACGGCGGAAAAGATATCGTGTTTCATGAATACGATTCTTATTGGCGCAGTATGCGAAAAATGTGCTCTTTTGAGCTGCTCAACACTCCTAAAATCAACTCCTTTAGATCCATGAGAAAGCAAGAGATCGGTTTTTTGGTCGATCGTCTCCGAGAGGCGGCGGGAAATCGTGTGGCGGTGGATCTTAACAATGAAGCTTCGTCTCTGATTATGGATGTAGTTTGTTTGATGGTGTTTGGGAAGAATTGTGGGGATGAGGAGTTTAAGAGTGGGATTCATGAGGCGTCGCGGTTGGCGGGAGCTCCTAACTTGGGGGATTTTATTCCTTTTCTTGCTTTGCTTGATTTGCAAGGATTAGGGCGTCGTGCGAAATGTGTTACTAAGAGAGACCGAGTTGAGTACCAATTTGATCGCTCCAGCATTAAAGCTATAATTCTCGATTTGATTATTGGAGCAGTGGACAGTTCAAGCACTGCAATAAATTGGGCATTACCAGAGCTCATCAAGCATCAACACATAATGAAGAAAGTGCAGGTGGAGTTAGAAAATGTGGTTGGTTTGGATAGGATGGTAGAAGAATCCGACTTGAGTCGCTTTGAAATATCTGGAGATGGTCATTAA

Coding sequence (CDS)

ATGGCTTGGATTTCAGCCACCATTATACTAACCCTCTTTCTTCTTGCTTGTCTCTTTCAGCCATGGCTGCCCAAGAAGAAGCTTCCTCCTGGCCCAAAAGGGCTTCCCATCATTGGCAGTCTCCATCTTTTAGGAAAACTTGTTCACAGAGATCTCCATAACCTCTCCCAAAGATACGGCCCCATAATGCACGTCCGGTTCGGGCTCGTCCCCGCCATCATCGTGTCTTCCCCTCACGCTTGCAAGCTCTTCCTCAAAACCCAGGACCCTGCATTTGCAGGTCGCCCACTGCCTCACACTTCCAACCAAATGTCCTACGGCGGAAAAGATATCGTGTTTCATGAATACGATTCTTATTGGCGCAGTATGCGAAAAATGTGCTCTTTTGAGCTGCTCAACACTCCTAAAATCAACTCCTTTAGATCCATGAGAAAGCAAGAGATCGGTTTTTTGGTCGATCGTCTCCGAGAGGCGGCGGGAAATCGTGTGGCGGTGGATCTTAACAATGAAGCTTCGTCTCTGATTATGGATGTAGTTTGTTTGATGGTGTTTGGGAAGAATTGTGGGGATGAGGAGTTTAAGAGTGGGATTCATGAGGCGTCGCGGTTGGCGGGAGCTCCTAACTTGGGGGATTTTATTCCTTTTCTTGCTTTGCTTGATTTGCAAGGATTAGGGCGTCGTGCGAAATGTGTTACTAAGAGAGACCGAGTTGAGTACCAATTTGATCGCTCCAGCATTAAAGCTATAATTCTCGATTTGATTATTGGAGCAGTGGACAGTTCAAGCACTGCAATAAATTGGGCATTACCAGAGCTCATCAAGCATCAACACATAATGAAGAAAGTGCAGGTGGAGTTAGAAAATGTGGTTGGTTTGGATAGGATGGTAGAAGAATCCGACTTGAGTCGCTTTGAAATATCTGGAGATGGTCATTAA

Protein sequence

MAWISATIILTLFLLACLFQPWLPKKKLPPGPKGLPIIGSLHLLGKLVHRDLHNLSQRYGPIMHVRFGLVPAIIVSSPHACKLFLKTQDPAFAGRPLPHTSNQMSYGGKDIVFHEYDSYWRSMRKMCSFELLNTPKINSFRSMRKQEIGFLVDRLREAAGNRVAVDLNNEASSLIMDVVCLMVFGKNCGDEEFKSGIHEASRLAGAPNLGDFIPFLALLDLQGLGRRAKCVTKRDRVEYQFDRSSIKAIILDLIIGAVDSSSTAINWALPELIKHQHIMKKVQVELENVVGLDRMVEESDLSRFEISGDGH
Homology
BLAST of Moc10g15230 vs. NCBI nr
Match: XP_038904224.1 (cytochrome P450 71AU50-like [Benincasa hispida])

HSP 1 Score: 367.1 bits (941), Expect = 1.5e-97
Identity = 194/344 (56.40%), Postives = 236/344 (68.60%), Query Frame = 0

Query: 1   MAWISATIILTLFLLACLFQPWLPK---KKLPPGPKGLPIIGSLHLLGKLVHRDLHNLSQ 60
           MA ISAT I TL LLAC+    L K   K LPPGPKG PIIGS+HLL KLVHRDL  LSQ
Sbjct: 1   MALISAT-IFTLILLACILHLLLCKIKAKNLPPGPKGFPIIGSIHLLKKLVHRDLQKLSQ 60

Query: 61  RYGPIMHVRFGLVPAIIVSSPHACKLFLKTQDPAFAGRPLPHTSNQMSYGGKDIVFHEYD 120
            YGPIMH++ G    II+SSP   KLFLKT DP FA RP+ HTSNQMS+  KDI F ++ 
Sbjct: 61  TYGPIMHMKLGFKSTIIISSPTIAKLFLKTHDPIFASRPISHTSNQMSFNRKDIAFAQFG 120

Query: 121 SYWRSMRKMCSFELLNTPKINSFRSMRKQEIGFLVDRLREAAGNRVAVDLNNEASSLIMD 180
            YW +MRK+CSF+LL + KI+SF S+R+QE+G L+DRLREAA N   VDLN++ SSLI  
Sbjct: 121 PYWHNMRKICSFQLLTSLKIDSFSSIRRQELGLLIDRLREAAKNHSIVDLNSQVSSLIAG 180

Query: 181 VVCLMVFGKNCGDEEFKSGIHEASRLAGAPNLGDFIPFLALLDLQGLGRRAKCVTK---- 240
           V+C+M+FGK   DEEF + IHE + LAGA NLGDFIPF+A LDLQGLGRR+K ++K    
Sbjct: 181 VICVMLFGKKFVDEEFMAAIHEVTFLAGALNLGDFIPFIAFLDLQGLGRRSKAISKAFDG 240

Query: 241 --------------------------------RDRVEYQFDRSSIKAIILDLIIGAVDSS 300
                                            ++ + Q DR+SIK ++ DLI+G VDS+
Sbjct: 241 FLNTIIEQHVEFKDQNNTKSGLFVDVMLDLMRSEKTKCQIDRTSIKGVMFDLIVGGVDST 300

Query: 301 STAINWALPELIKHQHIMKKVQVELENVVGLDRMVEESDLSRFE 306
           ST INWAL ELIKH  +MKKVQ ELE VVGL+RMVEESDL++ +
Sbjct: 301 STIINWALSELIKHPQVMKKVQKELEEVVGLNRMVEESDLNQLK 343

BLAST of Moc10g15230 vs. NCBI nr
Match: XP_023007802.1 (cytochrome P450 CYP736A12-like [Cucurbita maxima])

HSP 1 Score: 365.2 bits (936), Expect = 5.8e-97
Identity = 192/331 (58.01%), Postives = 236/331 (71.30%), Query Frame = 0

Query: 1   MAWISATIILTLFLLACLFQPWLPK---KKLPPGPKGLPIIGSLHLLGKLVHRDLHNLSQ 60
           MAW+SA I++++ LLA L   WL K   KKLPPGPKG PIIG++HLL KLVHRDLH+LSQ
Sbjct: 1   MAWVSAFIVISI-LLAFLLNSWLSKMNIKKLPPGPKGFPIIGTIHLLKKLVHRDLHHLSQ 60

Query: 61  RYGPIMHVRFGLVPAIIVSSPHACKLFLKTQDPAFAGRPLPHTSNQMSYGGKDIVFHEYD 120
            YGPIMH++ GL   IIVSS HA +LFLK  DP FAGRP+  T NQ+SY  K+I F +Y 
Sbjct: 61  LYGPIMHMKLGLKSTIIVSSSHAAELFLKAHDPVFAGRPVRLTFNQLSYHQKNIAFAQYG 120

Query: 121 SYWRSMRKMCSFELLNTPKINSFRSMRKQEIGFLVDRLREAAGNRVAVDLNNEASSLIMD 180
            YWR+MRK+C  +L  T K+NSF SMR++E+G L+D L+EAA     VDL+++ SS I D
Sbjct: 121 PYWRNMRKICILQLFTTLKMNSFTSMRREEVGLLIDHLQEAAKMHGVVDLSSQISSFIAD 180

Query: 181 VVCLMVFGKNCGDEEFKSGIHEASRLAGAPNLGDFIPFLALLDLQGLGRRAKCVTK---- 240
           V+C MVFG+   +EEFK+ IHEA+ L GAP+LGDFIPF+++LDLQGLGRRAK VTK    
Sbjct: 181 VICRMVFGRKFVEEEFKASIHEATLLVGAPDLGDFIPFISMLDLQGLGRRAKGVTKVFDG 240

Query: 241 ----------------RDR-------VEYQFDRSSIKAIILDLIIGAVDSSSTAINWALP 300
                           R +       ++ + D SSIK +I+DLIIG VDSS T I+WAL 
Sbjct: 241 FLDMIIKQHLESNDENRTKGCFLDVLLDSKIDHSSIKGVIMDLIIGGVDSSRTTIDWALS 300

Query: 301 ELIKHQHIMKKVQVELENVVGLDRMVEESDL 302
           ELIKH H MKKVQ ELENVVGL +MVEESDL
Sbjct: 301 ELIKHPHTMKKVQKELENVVGLGKMVEESDL 330

BLAST of Moc10g15230 vs. NCBI nr
Match: XP_023543453.1 (cytochrome P450 CYP736A12-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 363.6 bits (932), Expect = 1.7e-96
Identity = 204/348 (58.62%), Postives = 244/348 (70.11%), Query Frame = 0

Query: 1   MAWISATIILTLFLLACLFQPWLPK-----KKLPPGPKGLPIIGSLHLLGKLVHRDLHNL 60
           MA I AT+IL + LL  L Q W+ K      KLPPGPKG PIIGSLHLL KL+HRDLH+L
Sbjct: 1   MALILATMIL-ISLLTYLIQGWVFKSNTFGNKLPPGPKGFPIIGSLHLLSKLIHRDLHHL 60

Query: 61  SQRYGPIMHVRFGLVPAIIVSSPHACKLFLKTQDPAFAGRPLPHTSNQMSYGGKDIVFHE 120
           SQRYGPIM+++ G V AI+VSSP A +LFLKT D  F  RPL  TSNQ+SYG K I F +
Sbjct: 61  SQRYGPIMYMQLGRVHAIVVSSPRAAELFLKTHDLDFPSRPLTKTSNQISYGRKGIAFSQ 120

Query: 121 YDSYWRSMRKMCSFELLNTPKINSFRSMRKQEIGFLVDRLREAAGNRVAVDLNNEASSLI 180
           YDS+WR+MRKMC+ EL +  KINSF SMRKQE+  LVD L+EAA NRVAVDL+++ S LI
Sbjct: 121 YDSHWRNMRKMCTLELFSFLKINSFSSMRKQEVRLLVDDLQEAAENRVAVDLSSKISCLI 180

Query: 181 MDVVCLMVFGKNCGDEE-----FKSGIHEASRLAGAPNLGDFIPFLALLDLQGLGRRAK- 240
           +D++CLMV G+   D+E     FK+ I EA+RLA APNLGDF PF+A LDLQGL RRAK 
Sbjct: 181 VDIMCLMVVGRKYRDKELDAKGFKAVIQEATRLAAAPNLGDFFPFIARLDLQGLCRRAKS 240

Query: 241 ----------------CVTKRDR----------------VEYQFDRSSIKAIILDLIIGA 300
                            V+K D+                 +YQ D S+IKA+ILDL+I A
Sbjct: 241 VGDVFDGFLEKIVEEHVVSKNDKNKDFVDVMLDLMGSRENDYQIDCSTIKAMILDLLIAA 300

Query: 301 VDSSSTAINWALPELIKHQHIMKKVQVELENVVGLDRMVEESDLSRFE 306
           VDSS+T INWALPELIKH HIMKK+Q ELE VVGL+RMVEESDL+  +
Sbjct: 301 VDSSTTIINWALPELIKHPHIMKKMQEELEKVVGLNRMVEESDLNNLK 347

BLAST of Moc10g15230 vs. NCBI nr
Match: XP_022932265.1 (cytochrome P450 CYP736A12-like [Cucurbita moschata])

HSP 1 Score: 359.8 bits (922), Expect = 2.4e-95
Identity = 193/331 (58.31%), Postives = 233/331 (70.39%), Query Frame = 0

Query: 1   MAWISATIILTLFLLACLFQPWLPK---KKLPPGPKGLPIIGSLHLLGKLVHRDLHNLSQ 60
           M  ISA I++   LLA L   WL K   KKLPPGPKG PIIG++HLL KLVHRDLH+LSQ
Sbjct: 1   MPSISAFIVI-FILLAFLLHSWLSKMNIKKLPPGPKGFPIIGTIHLLKKLVHRDLHHLSQ 60

Query: 61  RYGPIMHVRFGLVPAIIVSSPHACKLFLKTQDPAFAGRPLPHTSNQMSYGGKDIVFHEYD 120
            YGPIMH++ GL   IIVSS HA +LFLK  DP FAGRP+  T NQ+SY  K+I F +Y 
Sbjct: 61  LYGPIMHMKLGLKSTIIVSSSHAAELFLKAHDPVFAGRPIRLTFNQLSYHQKNIAFAQYG 120

Query: 121 SYWRSMRKMCSFELLNTPKINSFRSMRKQEIGFLVDRLREAAGNRVAVDLNNEASSLIMD 180
            YWR+MRK+C  +L  T K+NSF SMR++E+G L+D L+EAA     VDL+++ SSLI D
Sbjct: 121 PYWRNMRKICILQLFTTLKMNSFTSMRREEVGLLIDHLQEAAKMHGVVDLSSKISSLIAD 180

Query: 181 VVCLMVFGKNCGDEEFKSGIHEASRLAGAPNLGDFIPFLALLDLQGLGRRAKCVTK---- 240
           V+C MVFG+   +EEFK+ IHEA+ L GAPNLGDFIPF+++LDL GLGRRAK VTK    
Sbjct: 181 VICRMVFGRKFVEEEFKASIHEATLLVGAPNLGDFIPFISMLDLHGLGRRAKGVTKVFDG 240

Query: 241 ----------------RDR-------VEYQFDRSSIKAIILDLIIGAVDSSSTAINWALP 300
                           R +       ++ + D SSIK +I+DLIIG VDSS TAI+WAL 
Sbjct: 241 FLDIIIKQHLESNDENRTKSCFLDVLLDSKIDHSSIKGVIMDLIIGGVDSSRTAIDWALS 300

Query: 301 ELIKHQHIMKKVQVELENVVGLDRMVEESDL 302
           ELIKH H MKKVQ ELENVVGL +MVEESDL
Sbjct: 301 ELIKHPHAMKKVQKELENVVGLGKMVEESDL 330

BLAST of Moc10g15230 vs. NCBI nr
Match: XP_008441066.1 (PREDICTED: cytochrome P450 CYP736A12-like [Cucumis melo] >TYK12655.1 cytochrome P450 CYP736A12-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 357.1 bits (915), Expect = 1.6e-94
Identity = 197/347 (56.77%), Postives = 234/347 (67.44%), Query Frame = 0

Query: 1   MAWISATIILTLFLLACLFQPWLPK-----KKLPPGPKGLPIIGSLHLLGKLVHRDLHNL 60
           M WI    I  +FLL  LFQ WL K      KLPPGPKG PIIGSLHLLGKL+H+DLH L
Sbjct: 1   MTWIILATITLIFLLPYLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYL 60

Query: 61  SQRYGPIMHVRFGLVPAIIVSSPHACKLFLKTQDPAFAGRPLPHTSNQMSYGGKDIVFHE 120
           SQ YGPIMH++ GL+PAIIVSS  A +LFLKT D  FA RPL  TSN +SYG K IVF +
Sbjct: 61  SQIYGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFASRPLTITSNHISYGRKGIVFAQ 120

Query: 121 YDSYWRSMRKMCSFELLNTPKINSFRSMRKQEIGFLVDRLREAAGNRVAVDLNNEASSLI 180
           Y  YWR++RKMC+ ELL++ KINSF SMRKQE+G L+  L +AA + VAVDL ++ SS+I
Sbjct: 121 YGPYWRNIRKMCTLELLSSLKINSFSSMRKQEVGSLIKGLEDAATDGVAVDLTSKISSVI 180

Query: 181 MDVVCLMVFGKNCGDEE-----FKSGIHEASRLAGAPNLGDFIPFLALLDLQGLGRRAKC 240
            D++C+MV G+   D E     FK  I EA++LA APNLGDFIP +A  D+QG G RAK 
Sbjct: 181 GDMICVMVLGRKYEDNELGEKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKA 240

Query: 241 VTK----------------------RDRV----------EYQFDRSSIKAIILDLIIGAV 300
           V K                      +D V          EYQ DRS+IKAIILD +I AV
Sbjct: 241 VGKIFDGFLESIVEEHVVFEKDNKDKDFVDVLLDLMGSREYQIDRSNIKAIILDFVIAAV 300

Query: 301 DSSSTAINWALPELIKHQHIMKKVQVELENVVGLDRMVEESDLSRFE 306
           DS++T INW L ELIKH HIMKK+Q ELE VVGL+RMVEESDLS  +
Sbjct: 301 DSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLK 347

BLAST of Moc10g15230 vs. ExPASy Swiss-Prot
Match: A0A068Q5V6 (Cytochrome P450 71AU50 OS=Prunus mume OX=102107 GN=CYP71AU50 PE=2 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 5.8e-84
Identity = 168/347 (48.41%), Postives = 223/347 (64.27%), Query Frame = 0

Query: 1   MAWISATIILTLFLLACLFQPWL--PKKKLPPGPKGLPIIGSLHLLGKLVHRDLHNLSQR 60
           M WI ATI   L  L  + Q W    KK+LPPGP+G PI GSLHLLG+  ++DLH L+++
Sbjct: 1   MVWIWATI--GLLALVHILQAWWKNKKKRLPPGPRGFPIFGSLHLLGEFPNKDLHRLARK 60

Query: 61  YGPIMHVRFGLVPAIIVSSPHACKLFLKTQDPAFAGRPLPHTSNQMSYGGKDIVFHEYDS 120
           YG IM++R GL+P I++SSP A +LFLKT D  FA RP    S  +S+G K+++F EY +
Sbjct: 61  YGDIMYMRLGLMPTIVISSPEAAELFLKTHDLVFASRPPHEGSKHISFGQKNLIFSEYGA 120

Query: 121 YWRSMRKMCSFELLNTPKINSFRSMRKQEIGFLVDRLREAAGNR-VAVDLNNEASSLIMD 180
           YWR  RKMC+ ELL+  KINSF+SMR++E+   V+ +R AA NR VAVDL+++ SSL +D
Sbjct: 121 YWRDTRKMCTIELLSNHKINSFKSMRREEVSLCVESIRAAANNRGVAVDLSDKVSSLSVD 180

Query: 181 VVCLMVFGKNCGDEE-----FKSGIHEASRLAGAPNLGDFIPFLALLDLQGLGRRAKCVT 240
           + C MV GK   DEE     FKS + EA +LA APNLGD+I F+A LDLQG  +R K V 
Sbjct: 181 MSCRMVLGKKYRDEEFDERGFKSVVREAIQLASAPNLGDYIRFIAPLDLQGFTKRMKSVN 240

Query: 241 K----------------------------------RDRVEYQFDRSSIKAIILDLIIGAV 300
           K                                   +  EY+ +R  IKAI+LD+++ ++
Sbjct: 241 KAFDNLFEKIIEEHLQPNDGERTMDFVDVMVGFMGSEESEYRIERPHIKAIMLDMLVASM 300

Query: 301 DSSSTAINWALPELIKHQHIMKKVQVELENVVGLDRMVEESDLSRFE 306
           D+S+T I WAL EL++H   MKKVQ ELENVVGLD+MVEESDL + +
Sbjct: 301 DTSATTIEWALSELMRHPKAMKKVQKELENVVGLDKMVEESDLEKLD 345

BLAST of Moc10g15230 vs. ExPASy Swiss-Prot
Match: H2DH18 (Cytochrome P450 CYP736A12 OS=Panax ginseng OX=4054 PE=2 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 3.6e-65
Identity = 139/329 (42.25%), Postives = 187/329 (56.84%), Query Frame = 0

Query: 18  LFQPWLPKKKLPPGPKGLPIIGSLHLLGKLVHRDLHNLSQRYGPIMHVRFGLVPAIIVSS 77
           +  P     KLPPGP+GLPIIGSLH LG L HR L  L+++YGPIM +R G VP I+VSS
Sbjct: 23  IISPLKRHHKLPPGPRGLPIIGSLHTLGALPHRTLQTLAKKYGPIMSMRLGSVPTIVVSS 82

Query: 78  PHACKLFLKTQDPAFAGRPLPHTSNQMSYGGKDIVFHEYDSYWRSMRKMCSFELLNTPKI 137
           P A +LFLKT D  FA RP    +  MSYG   + F  Y  +WR++RK    ELL   KI
Sbjct: 83  PQAAELFLKTHDNIFASRPKLQAAEYMSYGTMGMSFTAYGPHWRNIRKFVVLELLTPAKI 142

Query: 138 NSFRSMRKQEIGFLVDRLREAAGNRVAVDLNNEASSLIMDVVCLMVFGKNCGDE-EFKSG 197
           NSF  MR++E+G +V  ++EA+     VDL+ + +++I ++   ++ G+   D  + K  
Sbjct: 143 NSFVGMRREELGTVVKSIKEASAANEVVDLSAKVANIIENMTYRLLLGRTKDDRYDLKGI 202

Query: 198 IHEASRLAGAPNLGDFIPFLALLDLQGLGRRAKCVTKR---------------------- 257
           ++EA  LAG  N+ DF+PFL  LD+QGL R+ K   KR                      
Sbjct: 203 MNEALTLAGRFNIADFVPFLGPLDIQGLTRQFKDTGKRLDKILEFIIDEHEQNSSNGNAS 262

Query: 258 ------------------DRVEYQFDRSSIKAIILDLIIGAVDSSSTAINWALPELIKHQ 306
                             D +    DRS IKAI++D+I  A+D+S T+I W L ELIKH 
Sbjct: 263 GDFIDDMLSLKNKPSNTHDELSKVIDRSVIKAIMIDIISAAIDTSDTSIEWILTELIKHP 322

BLAST of Moc10g15230 vs. ExPASy Swiss-Prot
Match: Q50EK4 (Cytochrome P450 750A1 OS=Pinus taeda OX=3352 GN=CYP750A1 PE=2 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 4.7e-49
Identity = 117/351 (33.33%), Postives = 180/351 (51.28%), Query Frame = 0

Query: 4   ISATIILT-LFLLAC-LFQPWLPKKKLPPGPKGLPIIGSLHLLGKLVHRDLHNLSQRYGP 63
           + A +I T +F  +C +       ++LPPGP   PIIG+ H +   +HR L NL+++YGP
Sbjct: 16  LPAILIATFIFFFSCWILHQSQRNERLPPGPYPWPIIGNFHQVRLPLHRTLKNLAEKYGP 75

Query: 64  IMHVRFGLVPAIIVSSPHACKLFLKTQDPAFAGRPLPHTSNQMSYGGKDIVFHEYDSYWR 123
           I+ +RFG VP ++VSS    K FLKT D  FA RP         Y  KDI F  Y  +WR
Sbjct: 76  ILFLRFGSVPTVVVSSSEKAKHFLKTHDLIFASRPPTSVGKYFFYNFKDIAFSPYGDHWR 135

Query: 124 SMRKMCSFELLNTPKINSFRSMRKQEIGFLVDRL-REAAGNRVAVDLNNEASSLIMDVVC 183
            MRK+C  ELL + +I SF+ +R++E+  ++  +  E+   R+AV+++   S+ + +++ 
Sbjct: 136 KMRKICVLELLTSKRIESFKHVRQEELSAMIHSIWEESESGRIAVNVSKAISTSLANILW 195

Query: 184 LMVFGKNCGDEE-------FKSGIHEASRLAGAPNLGDFIPFLALLDLQGLGRRAK---- 243
            ++  K   D +       F   + E S   G+ N+GDFIP+L  LDLQG+ R  K    
Sbjct: 196 RILARKKFSDNDLGADGKGFADLVVEVSIAVGSLNIGDFIPYLDCLDLQGIKRALKKANA 255

Query: 244 ---------------------------C-----------VTKRDRVEYQFDRSSIKAIIL 303
                                      C           + K D    +  R  IKAI  
Sbjct: 256 RFDAFAEKMIDEHINASTIRNGEADAGCHVKDIIDVLLEMAKNDNTGAKVTREIIKAITY 315

BLAST of Moc10g15230 vs. ExPASy Swiss-Prot
Match: O81973 (Cytochrome P450 93A3 OS=Glycine max OX=3847 GN=CYP93A3 PE=2 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 7.4e-47
Identity = 108/326 (33.13%), Postives = 168/326 (51.53%), Query Frame = 0

Query: 25  KKKLPPGPKGLPIIGSLHLLGKLVHRDLHNLSQRYGPIMHVRFGLVPAIIVSSPHACKLF 84
           K  LPP P  LPIIG LHLL    H+D H LS RYGPI+H+  G VP ++ S+  A K F
Sbjct: 28  KTLLPPSPMPLPIIGHLHLLSPTPHQDFHKLSLRYGPIIHLFLGSVPCVVASTAEAAKEF 87

Query: 85  LKTQDPAFAGRPLPHTS-NQMSYGGKDIVFHEYDSYWRSMRKMCSFELLNTPKINSFRSM 144
           LKT +PAF+ RP    +   ++YG +D +F  Y  YW+ M+K+C  ELL    ++ F  +
Sbjct: 88  LKTHEPAFSNRPANTVAVETLTYGFQDFLFAPYGPYWKFMKKLCMSELLGGHMLDQFLPV 147

Query: 145 RKQEIGFLVDRLREAAGNRVAVDLNNEASSLIMDVVCLMVFGKNCGD------EEFKSGI 204
           R+ E    + R+ +   +  AVD   E  +L  ++V  M+  +          EE +  +
Sbjct: 148 RQXETKKFIKRVLQKGISGEAVDFGGEFITLSNNIVSRMIVSQTSTTEDENEVEEMRKLV 207

Query: 205 HEASRLAGAPNLGDFIPFLALLDLQGLGRRAKCV-----TKRDRV--------------- 264
            +A+ L+G  N+ DF+ FL   DLQG  +R + +     T  DR+               
Sbjct: 208 KDAAELSGKFNISDFVSFLKRFDLQGFNKRLEKIRDCFDTVLDRIIKQREEERRNKNETV 267

Query: 265 ---------------------EYQFDRSSIKAIILDLIIGAVDSSSTAINWALPELIKHQ 303
                                E + ++ +IKA ILD++I   D+S+  + WA+ ELI + 
Sbjct: 268 GKREFKDMLDVLFDISEDESSEIKLNKENIKAFILDILIAGTDTSAVTMEWAMAELINNP 327

BLAST of Moc10g15230 vs. ExPASy Swiss-Prot
Match: P24465 (Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2)

HSP 1 Score: 188.3 bits (477), Expect = 1.3e-46
Identity = 116/339 (34.22%), Postives = 175/339 (51.62%), Query Frame = 0

Query: 8   IILTLFLLACLFQPWLPKKKLPPGPKGLPIIGSLHLLGKLVHRDLHNLSQRYGPIMHVRF 67
           I LT FLL  L +    K  LPP P  LPIIG+LH LG L HR L +L+   GP++ +  
Sbjct: 12  IALTFFLLK-LNEKREKKPNLPPSPPNLPIIGNLHQLGNLPHRSLRSLANELGPLILLHL 71

Query: 68  GLVPAIIVSSPHACKLFLKTQDPAFAGRPLPHTSNQMSYGGKDIVFHEYDSYWRSMRKMC 127
           G +P +IVS+    +  LKT D  FA RP    + ++ Y   D+ F  Y  YWR +RK+C
Sbjct: 72  GHIPTLIVSTAEIAEEILKTHDLIFASRPSTTAARRIFYDCTDVAFSPYGEYWRQVRKIC 131

Query: 128 SFELLNTPKINSFRSMRKQEIGFLVDRLREAAGNRVAVDLNNEASSLIMDVVCLMVFGKN 187
             ELL+  ++NS+RS+R++E+G +++R+ ++     AV+L+     L    +  + FGK 
Sbjct: 132 VLELLSIKRVNSYRSIREEEVGLMMERISQSCSTGEAVNLSELLLLLSSGTITRVAFGKK 191

Query: 188 C-GDEEFKSGI----HEASRLAGAPNLGDFIPFLALLD-LQGLGRRAK------------ 247
             G+EE K+       E + L GA  +GD+ P  A +D L G+  R K            
Sbjct: 192 YEGEEERKNKFADLATELTTLMGAFFVGDYFPSFAWVDVLTGMDARLKRNHGELDAFVDH 251

Query: 248 ---------------CVTKRDRVE------------YQFDRSSIKAIILDLIIGAVDSSS 302
                           V ++D V+               +R+++KA+ILD+  G  D+++
Sbjct: 252 VIDDHLLSRKANGSDGVEQKDLVDVLLHLQKDSSLGVHLNRNNLKAVILDMFSGGTDTTA 311

BLAST of Moc10g15230 vs. ExPASy TrEMBL
Match: A0A6J1KZP5 (cytochrome P450 CYP736A12-like OS=Cucurbita maxima OX=3661 GN=LOC111500301 PE=3 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 2.8e-97
Identity = 192/331 (58.01%), Postives = 236/331 (71.30%), Query Frame = 0

Query: 1   MAWISATIILTLFLLACLFQPWLPK---KKLPPGPKGLPIIGSLHLLGKLVHRDLHNLSQ 60
           MAW+SA I++++ LLA L   WL K   KKLPPGPKG PIIG++HLL KLVHRDLH+LSQ
Sbjct: 1   MAWVSAFIVISI-LLAFLLNSWLSKMNIKKLPPGPKGFPIIGTIHLLKKLVHRDLHHLSQ 60

Query: 61  RYGPIMHVRFGLVPAIIVSSPHACKLFLKTQDPAFAGRPLPHTSNQMSYGGKDIVFHEYD 120
            YGPIMH++ GL   IIVSS HA +LFLK  DP FAGRP+  T NQ+SY  K+I F +Y 
Sbjct: 61  LYGPIMHMKLGLKSTIIVSSSHAAELFLKAHDPVFAGRPVRLTFNQLSYHQKNIAFAQYG 120

Query: 121 SYWRSMRKMCSFELLNTPKINSFRSMRKQEIGFLVDRLREAAGNRVAVDLNNEASSLIMD 180
            YWR+MRK+C  +L  T K+NSF SMR++E+G L+D L+EAA     VDL+++ SS I D
Sbjct: 121 PYWRNMRKICILQLFTTLKMNSFTSMRREEVGLLIDHLQEAAKMHGVVDLSSQISSFIAD 180

Query: 181 VVCLMVFGKNCGDEEFKSGIHEASRLAGAPNLGDFIPFLALLDLQGLGRRAKCVTK---- 240
           V+C MVFG+   +EEFK+ IHEA+ L GAP+LGDFIPF+++LDLQGLGRRAK VTK    
Sbjct: 181 VICRMVFGRKFVEEEFKASIHEATLLVGAPDLGDFIPFISMLDLQGLGRRAKGVTKVFDG 240

Query: 241 ----------------RDR-------VEYQFDRSSIKAIILDLIIGAVDSSSTAINWALP 300
                           R +       ++ + D SSIK +I+DLIIG VDSS T I+WAL 
Sbjct: 241 FLDMIIKQHLESNDENRTKGCFLDVLLDSKIDHSSIKGVIMDLIIGGVDSSRTTIDWALS 300

Query: 301 ELIKHQHIMKKVQVELENVVGLDRMVEESDL 302
           ELIKH H MKKVQ ELENVVGL +MVEESDL
Sbjct: 301 ELIKHPHTMKKVQKELENVVGLGKMVEESDL 330

BLAST of Moc10g15230 vs. ExPASy TrEMBL
Match: A0A6J1F1R2 (cytochrome P450 CYP736A12-like OS=Cucurbita moschata OX=3662 GN=LOC111438620 PE=3 SV=1)

HSP 1 Score: 359.8 bits (922), Expect = 1.2e-95
Identity = 193/331 (58.31%), Postives = 233/331 (70.39%), Query Frame = 0

Query: 1   MAWISATIILTLFLLACLFQPWLPK---KKLPPGPKGLPIIGSLHLLGKLVHRDLHNLSQ 60
           M  ISA I++   LLA L   WL K   KKLPPGPKG PIIG++HLL KLVHRDLH+LSQ
Sbjct: 1   MPSISAFIVI-FILLAFLLHSWLSKMNIKKLPPGPKGFPIIGTIHLLKKLVHRDLHHLSQ 60

Query: 61  RYGPIMHVRFGLVPAIIVSSPHACKLFLKTQDPAFAGRPLPHTSNQMSYGGKDIVFHEYD 120
            YGPIMH++ GL   IIVSS HA +LFLK  DP FAGRP+  T NQ+SY  K+I F +Y 
Sbjct: 61  LYGPIMHMKLGLKSTIIVSSSHAAELFLKAHDPVFAGRPIRLTFNQLSYHQKNIAFAQYG 120

Query: 121 SYWRSMRKMCSFELLNTPKINSFRSMRKQEIGFLVDRLREAAGNRVAVDLNNEASSLIMD 180
            YWR+MRK+C  +L  T K+NSF SMR++E+G L+D L+EAA     VDL+++ SSLI D
Sbjct: 121 PYWRNMRKICILQLFTTLKMNSFTSMRREEVGLLIDHLQEAAKMHGVVDLSSKISSLIAD 180

Query: 181 VVCLMVFGKNCGDEEFKSGIHEASRLAGAPNLGDFIPFLALLDLQGLGRRAKCVTK---- 240
           V+C MVFG+   +EEFK+ IHEA+ L GAPNLGDFIPF+++LDL GLGRRAK VTK    
Sbjct: 181 VICRMVFGRKFVEEEFKASIHEATLLVGAPNLGDFIPFISMLDLHGLGRRAKGVTKVFDG 240

Query: 241 ----------------RDR-------VEYQFDRSSIKAIILDLIIGAVDSSSTAINWALP 300
                           R +       ++ + D SSIK +I+DLIIG VDSS TAI+WAL 
Sbjct: 241 FLDIIIKQHLESNDENRTKSCFLDVLLDSKIDHSSIKGVIMDLIIGGVDSSRTAIDWALS 300

Query: 301 ELIKHQHIMKKVQVELENVVGLDRMVEESDL 302
           ELIKH H MKKVQ ELENVVGL +MVEESDL
Sbjct: 301 ELIKHPHAMKKVQKELENVVGLGKMVEESDL 330

BLAST of Moc10g15230 vs. ExPASy TrEMBL
Match: A0A5D3CM91 (Cytochrome P450 CYP736A12-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G002430 PE=3 SV=1)

HSP 1 Score: 357.1 bits (915), Expect = 7.6e-95
Identity = 197/347 (56.77%), Postives = 234/347 (67.44%), Query Frame = 0

Query: 1   MAWISATIILTLFLLACLFQPWLPK-----KKLPPGPKGLPIIGSLHLLGKLVHRDLHNL 60
           M WI    I  +FLL  LFQ WL K      KLPPGPKG PIIGSLHLLGKL+H+DLH L
Sbjct: 1   MTWIILATITLIFLLPYLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYL 60

Query: 61  SQRYGPIMHVRFGLVPAIIVSSPHACKLFLKTQDPAFAGRPLPHTSNQMSYGGKDIVFHE 120
           SQ YGPIMH++ GL+PAIIVSS  A +LFLKT D  FA RPL  TSN +SYG K IVF +
Sbjct: 61  SQIYGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFASRPLTITSNHISYGRKGIVFAQ 120

Query: 121 YDSYWRSMRKMCSFELLNTPKINSFRSMRKQEIGFLVDRLREAAGNRVAVDLNNEASSLI 180
           Y  YWR++RKMC+ ELL++ KINSF SMRKQE+G L+  L +AA + VAVDL ++ SS+I
Sbjct: 121 YGPYWRNIRKMCTLELLSSLKINSFSSMRKQEVGSLIKGLEDAATDGVAVDLTSKISSVI 180

Query: 181 MDVVCLMVFGKNCGDEE-----FKSGIHEASRLAGAPNLGDFIPFLALLDLQGLGRRAKC 240
            D++C+MV G+   D E     FK  I EA++LA APNLGDFIP +A  D+QG G RAK 
Sbjct: 181 GDMICVMVLGRKYEDNELGEKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKA 240

Query: 241 VTK----------------------RDRV----------EYQFDRSSIKAIILDLIIGAV 300
           V K                      +D V          EYQ DRS+IKAIILD +I AV
Sbjct: 241 VGKIFDGFLESIVEEHVVFEKDNKDKDFVDVLLDLMGSREYQIDRSNIKAIILDFVIAAV 300

Query: 301 DSSSTAINWALPELIKHQHIMKKVQVELENVVGLDRMVEESDLSRFE 306
           DS++T INW L ELIKH HIMKK+Q ELE VVGL+RMVEESDLS  +
Sbjct: 301 DSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLK 347

BLAST of Moc10g15230 vs. ExPASy TrEMBL
Match: A0A1S3B2K5 (cytochrome P450 CYP736A12-like OS=Cucumis melo OX=3656 GN=LOC103485291 PE=3 SV=1)

HSP 1 Score: 357.1 bits (915), Expect = 7.6e-95
Identity = 197/347 (56.77%), Postives = 234/347 (67.44%), Query Frame = 0

Query: 1   MAWISATIILTLFLLACLFQPWLPK-----KKLPPGPKGLPIIGSLHLLGKLVHRDLHNL 60
           M WI    I  +FLL  LFQ WL K      KLPPGPKG PIIGSLHLLGKL+H+DLH L
Sbjct: 1   MTWIILATITLIFLLPYLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYL 60

Query: 61  SQRYGPIMHVRFGLVPAIIVSSPHACKLFLKTQDPAFAGRPLPHTSNQMSYGGKDIVFHE 120
           SQ YGPIMH++ GL+PAIIVSS  A +LFLKT D  FA RPL  TSN +SYG K IVF +
Sbjct: 61  SQIYGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFASRPLTITSNHISYGRKGIVFAQ 120

Query: 121 YDSYWRSMRKMCSFELLNTPKINSFRSMRKQEIGFLVDRLREAAGNRVAVDLNNEASSLI 180
           Y  YWR++RKMC+ ELL++ KINSF SMRKQE+G L+  L +AA + VAVDL ++ SS+I
Sbjct: 121 YGPYWRNIRKMCTLELLSSLKINSFSSMRKQEVGSLIKGLEDAATDGVAVDLTSKISSVI 180

Query: 181 MDVVCLMVFGKNCGDEE-----FKSGIHEASRLAGAPNLGDFIPFLALLDLQGLGRRAKC 240
            D++C+MV G+   D E     FK  I EA++LA APNLGDFIP +A  D+QG G RAK 
Sbjct: 181 GDMICVMVLGRKYEDNELGEKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKA 240

Query: 241 VTK----------------------RDRV----------EYQFDRSSIKAIILDLIIGAV 300
           V K                      +D V          EYQ DRS+IKAIILD +I AV
Sbjct: 241 VGKIFDGFLESIVEEHVVFEKDNKDKDFVDVLLDLMGSREYQIDRSNIKAIILDFVIAAV 300

Query: 301 DSSSTAINWALPELIKHQHIMKKVQVELENVVGLDRMVEESDLSRFE 306
           DS++T INW L ELIKH HIMKK+Q ELE VVGL+RMVEESDLS  +
Sbjct: 301 DSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLK 347

BLAST of Moc10g15230 vs. ExPASy TrEMBL
Match: A0A5A7T3X6 (Cytochrome P450 CYP736A12-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold18G00600 PE=3 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 1.1e-93
Identity = 195/347 (56.20%), Postives = 232/347 (66.86%), Query Frame = 0

Query: 1   MAWISATIILTLFLLACLFQPWLPK-----KKLPPGPKGLPIIGSLHLLGKLVHRDLHNL 60
           M WI    I  +FLL  LFQ WL K      KLPPGPKG PIIGSLHLLGKL+H+DLH L
Sbjct: 1   MTWIILATITLIFLLPYLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYL 60

Query: 61  SQRYGPIMHVRFGLVPAIIVSSPHACKLFLKTQDPAFAGRPLPHTSNQMSYGGKDIVFHE 120
           SQ YGPIMH++ GL+PAIIVSS  A +LFLKT D  FA RPL  TSN +SYG K I F +
Sbjct: 61  SQIYGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFASRPLTITSNHISYGRKGIAFAQ 120

Query: 121 YDSYWRSMRKMCSFELLNTPKINSFRSMRKQEIGFLVDRLREAAGNRVAVDLNNEASSLI 180
           Y  YWR++RKMC+ ELL++ KINSF S RKQE+G L+  L +AA + VAVDL ++ SS+I
Sbjct: 121 YGPYWRNIRKMCTLELLSSLKINSFSSTRKQEVGSLIKGLEDAATDGVAVDLTSKISSVI 180

Query: 181 MDVVCLMVFGKNCGDEE-----FKSGIHEASRLAGAPNLGDFIPFLALLDLQGLGRRAKC 240
            D++C+MV G+   D E     FK  I EA++LA APNLGDFIP +A  D+QG G RAK 
Sbjct: 181 GDMICVMVLGRKYEDNELGEKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKA 240

Query: 241 VTK----------------------RDRV----------EYQFDRSSIKAIILDLIIGAV 300
           V K                      +D V          EYQ DRS+IKAIILD +I AV
Sbjct: 241 VGKIFDGFLESIVEEHVVFEKDNKDKDFVDVLLDLMGSREYQIDRSNIKAIILDFVIAAV 300

Query: 301 DSSSTAINWALPELIKHQHIMKKVQVELENVVGLDRMVEESDLSRFE 306
           DS++T INW L ELIKH HIMKK+Q ELE VVGL+RMVEESDLS  +
Sbjct: 301 DSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLK 347

BLAST of Moc10g15230 vs. TAIR 10
Match: AT3G26310.1 (cytochrome P450, family 71, subfamily B, polypeptide 35 )

HSP 1 Score: 187.2 bits (474), Expect = 2.0e-47
Identity = 112/347 (32.28%), Postives = 178/347 (51.30%), Query Frame = 0

Query: 3   WISATIILTLFLLACLFQPWLPKKKLPPGPKGLPIIGSLHLLGKLVHRDLHNLSQRYGPI 62
           W+   I L   LLA       PK +  P P G PIIG+LH +G+L H+ L  LS++YGP+
Sbjct: 5   WLLPLIFLVCILLAVFNHKKHPKYRQFPCPPGFPIIGNLHQIGELPHQTLWKLSKKYGPV 64

Query: 63  MHVRFGLVPAIIVSSPHACKLFLKTQDPAFAGRPLPHTSNQMSYGGKDIVFHEYDSYWRS 122
           MH+  G VP ++VSS    +  L+  D     RP      ++SY   DI F  YD YW+ 
Sbjct: 65  MHLMLGRVPTVVVSSSDTARQVLRVHDLHCCTRPSLSGPRELSYNYLDIAFSPYDDYWKE 124

Query: 123 MRKMCSFELLNTPKINSFRSMRKQEIGFLVDRLREAAGNRVAVDLNNEASSLIMDVVCLM 182
           +RK+C  EL +T +++S + ++ +E+  ++D + E+A  +  V+LNN+   L + VVC  
Sbjct: 125 VRKLCVQELFSTKQVHSIQPIKDEEVKKMIDSIAESASQKNPVNLNNKCLELTVSVVCRT 184

Query: 183 VFGKN-----CGDEEFKSGIHEASRLAGAPNLGDFIPFLA-LLD-LQGL-GRRAKCVTKR 242
            FG +        + F   + EA  + G+ +  DFIP++  ++D L GL GRR +  +KR
Sbjct: 185 AFGVSFEGTVLNSDRFNKIVREALEMLGSFSAADFIPYVGWIIDVLTGLQGRRER--SKR 244

Query: 243 DR---VEYQFD---------------------------------RSSIKAIILDLIIGAV 302
           D     E  FD                                 R+ IKAI+LD+++  +
Sbjct: 245 DLNAFFEQMFDLHKEGKKEGNEDFVDLLLRLEKEEAVLGNDKLTRNHIKAILLDVLLAGI 304

Query: 303 DSSSTAINWALPELIKHQHIMKKVQVELENVVGLDRMVEESDLSRFE 306
           D+S+  + WA+ EL ++  +MKKVQ E+   +G   M+   D+ + E
Sbjct: 305 DTSAITMTWAMTELARNPRVMKKVQSEIRTQMGNRSMISFEDMDQLE 349

BLAST of Moc10g15230 vs. TAIR 10
Match: AT2G30750.1 (cytochrome P450, family 71, subfamily A, polypeptide 12 )

HSP 1 Score: 183.7 bits (465), Expect = 2.2e-46
Identity = 108/327 (33.03%), Postives = 168/327 (51.38%), Query Frame = 0

Query: 1   MAWISATIILTLFLLACLFQPWLPKKKLPPGPKGLPIIGSLHLLGKLVHRDLHNLSQRYG 60
           M  +  T ++TLFLL    +    K  LPP P  LP+IG+LH L    HR LH+LS RYG
Sbjct: 12  MVSLCLTTLITLFLLKQFLKRTANKVNLPPSPWRLPLIGNLHQLSLHPHRSLHSLSLRYG 71

Query: 61  PIMHVRFGLVPAIIVSSPHACKLFLKTQDPAFAGRPLPHTSNQMSYGGKDIVFHEYDSYW 120
           P+M + FG VP ++VSS  A +  LKT D  FA RP     + +  GG+D+VF  Y  YW
Sbjct: 72  PLMLLHFGRVPILVVSSGEAAQEVLKTHDLKFANRPRSKAVHGLMNGGRDVVFGPYGEYW 131

Query: 121 RSMRKMCSFELLNTPKINSFRSMRKQEIGFLVDRLREAAGNRVAVDLNNEASSLIMDVVC 180
           R M+ +C   LL    + SF  +R++E+  ++ +L +A+ +  + +L+    +L  DV  
Sbjct: 132 RQMKSVCILNLLTNKMVASFEKIREEELNEMIKKLEKASSSSSSENLSELFVTLPSDVTS 191

Query: 181 LMVFGKNCGDEE----FKSGIHEASRLAGAPNLGDFIPFLALLD-LQGLGRRAKCVT--- 240
            +  G+   ++E     K  + +   L G   +GD++P LA +D + G   R K V+   
Sbjct: 192 RIALGRKHSEDETARDLKKRVRQIMELLGEFPIGDYVPALAWIDRINGFNARIKEVSQGF 251

Query: 241 -----------------KRDRVE------------YQFDRSSIKAIILDLIIGAVDSSST 291
                            K D V+            +Q  R  IK +ILD+ IG   +SST
Sbjct: 252 SDLMDKVVQEHLEAGNHKEDFVDILLSIESEKSIGFQAQRDDIKFMILDMFIGGTSTSST 311

BLAST of Moc10g15230 vs. TAIR 10
Match: AT3G48280.1 (cytochrome P450, family 71, subfamily A, polypeptide 25 )

HSP 1 Score: 183.0 bits (463), Expect = 3.8e-46
Identity = 103/312 (33.01%), Postives = 163/312 (52.24%), Query Frame = 0

Query: 25  KKKLPPGPKGLPIIGSLHLLGKLVHRDLHNLSQRYGPIMHVRFGLVPAIIVSSPHACKLF 84
           K K PP P GLP+IG+LH LG+  HR L +LS+RYGP+M +  G VP +IVSS    +  
Sbjct: 27  KGKTPPSPPGLPLIGNLHQLGRHTHRSLCDLSRRYGPLMLLHLGRVPVLIVSSADMAQEI 86

Query: 85  LKTQDPAFAGRPLPHTSNQMSYGGKDIVFHEYDSYWRSMRKMCSFELLNTPKINSFRSMR 144
           LKT D AFA RP    S ++ Y  +D+    Y  YWR M+ +C   LL+   + SFR +R
Sbjct: 87  LKTHDQAFANRPRSKLSQKLLYNNRDVASAPYGEYWRQMKSVCVIHLLSNKMVRSFRDVR 146

Query: 145 KQEIGFLVDRLREAAGNRVAVDLNNEASSLIMDVVCLMVFGKNCGDE-EFKSGIHEASRL 204
           ++EI  ++ ++R+++   +  +++     L  DV+C +  G+  G E +FK      S L
Sbjct: 147 EEEITLMMAKIRKSSS--LPFNVSKVLECLTNDVICRVALGRKYGGETDFKKLTDRLSEL 206

Query: 205 AGAPNLGDFIPFLALLD--------LQGLGR-------------------------RAKC 264
            G  ++G F+P+LA +D        L  +G+                          A  
Sbjct: 207 LGTFSIGSFVPWLAWVDWIRGWDAQLDKMGKDLDDFFEKVVQDHEDGDRRDGTDLIDALL 266

Query: 265 VTKRDRVE-YQFDRSSIKAIILDLIIGAVDSSSTAINWALPELIKHQHIMKKVQVELENV 302
             KR++   ++ +R SIKAI LD+ +G  D+S T + WA+ EL++H   + ++Q E+  +
Sbjct: 267 RVKREKSPGFEIERVSIKAITLDVFVGGSDTSFTLLEWAMTELLRHPKSLNRLQEEVRTI 326

BLAST of Moc10g15230 vs. TAIR 10
Match: AT5G06900.1 (cytochrome P450, family 93, subfamily D, polypeptide 1 )

HSP 1 Score: 182.6 bits (462), Expect = 4.9e-46
Identity = 110/318 (34.59%), Postives = 161/318 (50.63%), Query Frame = 0

Query: 28  LPPGPKGLPIIGSLHLLGKLVHRDLHNLSQRYGPIMHVRFGLVPAIIVSSPHACKLFLKT 87
           LPP P  LPIIG +HLLG + H+ LH LS RYGP+M++  G +P +IVSS       LK+
Sbjct: 34  LPPSPTALPIIGHIHLLGPIAHQALHKLSIRYGPLMYLFIGSIPNLIVSSAEMANEILKS 93

Query: 88  QDPAFAGRPLPHTSNQMSYGGKDIVFHEYDSYWRSMRKMCSFELLNTPKINSFRSMRKQE 147
            +  F  RP     + ++YG  D     Y  +W+ M+++C  EL ++  ++SF S+R +E
Sbjct: 94  NELNFLNRPTMQNVDYLTYGSADFFSAPYGLHWKFMKRICMVELFSSRALDSFVSVRSEE 153

Query: 148 IGFLVDRLREAAGNRVAVDLNNEASSLIMDVVCLMVFGKNCGD-------EEFKSGIHEA 207
           +  L+ R+ + A    +V+L  +   L  +++  M+F K   D       EE    + E 
Sbjct: 154 LKKLLIRVLKKAEAEESVNLGEQLKELTSNIITRMMFRKMQSDSDGGEKSEEVIKMVVEL 213

Query: 208 SRLAGAPNLGDFIPFLALLDLQGLGRRAKCVTKRDR------------------------ 267
           + LAG  N+ +   FL  LDLQGL +R K    RD+                        
Sbjct: 214 NELAGFFNVSETFWFLKRLDLQGLKKRLK--NARDKYDVIIERIMEEHESSKKNATGERN 273

Query: 268 -------------VEYQFDRSSIKAIILDLIIGAVDSSSTAINWALPELIKHQHIMKKVQ 302
                         E +  R +IKA I+++  G  D+S+  + WAL ELI H  IMKK Q
Sbjct: 274 MLDVLLDIYEDKNAEMKLTRENIKAFIMNIYGGGTDTSAITVEWALAELINHPEIMKKAQ 333

BLAST of Moc10g15230 vs. TAIR 10
Match: AT5G57260.1 (cytochrome P450, family 71, subfamily B, polypeptide 10 )

HSP 1 Score: 182.6 bits (462), Expect = 4.9e-46
Identity = 107/332 (32.23%), Postives = 179/332 (53.92%), Query Frame = 0

Query: 3   WISATIILTLFLLACLFQPWLPKKKLPPGPKGLPIIGSLHLLGKLVHRDLHNLSQRYGPI 62
           W  + I+L   LL  +        + PP P GLPIIG+LH LG+L H+ L  LS++YGP+
Sbjct: 5   WFVSLILLISILLVAVKHSKRRWVRQPPSPPGLPIIGNLHQLGELPHQSLCKLSKKYGPV 64

Query: 63  MHVRFGLVPAIIVSSPHACKLFLKTQDPAFAGRPLPHTSNQMSYGGKDIVFHEYDSYWRS 122
           M ++ G VP +IVS+P   K  LK  D     RP    + ++SY   DI F  +D YW+ 
Sbjct: 65  MLLKLGRVPTVIVSTPETAKQVLKDYDLHCCSRPSLEGTRKLSYNYLDIAFSRFDDYWKE 124

Query: 123 MRKMCSFELLNTPKINSFRSMRKQEIGFLVDRLREAAGNRVAVDLNNEASSLIMDVVCLM 182
           +RK+C  EL    +INS + +++ E+  L+D + E+A  +  V+L++   SL ++V+C  
Sbjct: 125 LRKLCVEELFCNKRINSIQPIKEAEMEKLIDSIAESASQKTLVNLSDTFLSLNVNVICKA 184

Query: 183 VFGKN-----CGDEEFKSGIHEASRLAGAPNLGDFIPFLA-LLD-LQGL-GRRAKCV--- 242
           VFG N       +++F+  +HEA  + G+ +  DF P++  ++D   GL  RR + V   
Sbjct: 185 VFGVNFQGTVLNNDKFQDLVHEALEMLGSFSASDFFPYVGWIVDWFTGLHARRERSVRDL 244

Query: 243 -----------TKRDRVEYQFD---------------------RSSIKAIILDLIIGAVD 292
                       +++R E + D                     R+ IKAI++++++G ++
Sbjct: 245 DAFYEQMIDLHLQKNREESEDDFVDLLLRLEKEEAVLGYGKLTRNHIKAILMNILLGGIN 304

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038904224.11.5e-9756.40cytochrome P450 71AU50-like [Benincasa hispida][more]
XP_023007802.15.8e-9758.01cytochrome P450 CYP736A12-like [Cucurbita maxima][more]
XP_023543453.11.7e-9658.62cytochrome P450 CYP736A12-like [Cucurbita pepo subsp. pepo][more]
XP_022932265.12.4e-9558.31cytochrome P450 CYP736A12-like [Cucurbita moschata][more]
XP_008441066.11.6e-9456.77PREDICTED: cytochrome P450 CYP736A12-like [Cucumis melo] >TYK12655.1 cytochrome ... [more]
Match NameE-valueIdentityDescription
A0A068Q5V65.8e-8448.41Cytochrome P450 71AU50 OS=Prunus mume OX=102107 GN=CYP71AU50 PE=2 SV=1[more]
H2DH183.6e-6542.25Cytochrome P450 CYP736A12 OS=Panax ginseng OX=4054 PE=2 SV=1[more]
Q50EK44.7e-4933.33Cytochrome P450 750A1 OS=Pinus taeda OX=3352 GN=CYP750A1 PE=2 SV=1[more]
O819737.4e-4733.13Cytochrome P450 93A3 OS=Glycine max OX=3847 GN=CYP93A3 PE=2 SV=1[more]
P244651.3e-4634.22Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1KZP52.8e-9758.01cytochrome P450 CYP736A12-like OS=Cucurbita maxima OX=3661 GN=LOC111500301 PE=3 ... [more]
A0A6J1F1R21.2e-9558.31cytochrome P450 CYP736A12-like OS=Cucurbita moschata OX=3662 GN=LOC111438620 PE=... [more]
A0A5D3CM917.6e-9556.77Cytochrome P450 CYP736A12-like protein OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3B2K57.6e-9556.77cytochrome P450 CYP736A12-like OS=Cucumis melo OX=3656 GN=LOC103485291 PE=3 SV=1[more]
A0A5A7T3X61.1e-9356.20Cytochrome P450 CYP736A12-like protein OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
Match NameE-valueIdentityDescription
AT3G26310.12.0e-4732.28cytochrome P450, family 71, subfamily B, polypeptide 35 [more]
AT2G30750.12.2e-4633.03cytochrome P450, family 71, subfamily A, polypeptide 12 [more]
AT3G48280.13.8e-4633.01cytochrome P450, family 71, subfamily A, polypeptide 25 [more]
AT5G06900.14.9e-4634.59cytochrome P450, family 93, subfamily D, polypeptide 1 [more]
AT5G57260.14.9e-4632.23cytochrome P450, family 71, subfamily B, polypeptide 10 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 265..291
score: 34.24
coord: 56..75
score: 30.08
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 236..306
e-value: 5.6E-11
score: 43.6
coord: 23..235
e-value: 6.7E-42
score: 145.6
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 29..304
IPR001128Cytochrome P450PFAMPF00067p450coord: 239..303
e-value: 4.5E-11
score: 42.2
coord: 29..231
e-value: 1.0E-27
score: 97.1
NoneNo IPR availablePANTHERPTHR47943CYTOCHROME P450 93A3-LIKEcoord: 234..304
NoneNo IPR availablePANTHERPTHR47943CYTOCHROME P450 93A3-LIKEcoord: 2..233
NoneNo IPR availablePANTHERPTHR47943:SF2CYTOCHROME P450 FAMILY 71 PROTEINcoord: 2..233
coord: 234..304

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc10g15230.1Moc10g15230.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen