Moc10g09390 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc10g09390
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	RNase H domain-containing protein
Location	chr10: 6989588 .. 6989887 (-)
RNA-Seq Expression	Moc10g09390
Synteny	Moc10g09390

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAACGTTTGTGCACCTTTGAGGATGGAATCAAAGTTCAGGTGAAATCGGATTGCTTGGAAGTCGTGAACATCGTCAATAATTTCTTTGTGGAGCTAACTGAGACCTCTTTGTTGGCGGAAGATATTCGGAAGGTGGCGGATTTCATGCCAATTGAAAGATTTCACCACATTTCGAGGAAGGCGAACAGAACAGCTCACAGCTTGGCGAGGAAAGCATGCGAAGAGGGTTTATCGTTTGTGTGGTCGCTGAACTTCCCTTCGTGGCTCTCTAATTTGTTTCTTGTTGACAACGTTTAA

mRNA sequence

ATGAAACGTTTGTGCACCTTTGAGGATGGAATCAAAGTTCAGGTGAAATCGGATTGCTTGGAAGTCGTGAACATCGTCAATAATTTCTTTGTGGAGCTAACTGAGACCTCTTTGTTGGCGGAAGATATTCGGAAGGTGGCGGATTTCATGCCAATTGAAAGATTTCACCACATTTCGAGGAAGGCGAACAGAACAGCTCACAGCTTGGCGAGGAAAGCATGCGAAGAGGGTTTATCGTTTGTGTGGTCGCTGAACTTCCCTTCGTGGCTCTCTAATTTGTTTCTTGTTGACAACGTTTAA

Coding sequence (CDS)

ATGAAACGTTTGTGCACCTTTGAGGATGGAATCAAAGTTCAGGTGAAATCGGATTGCTTGGAAGTCGTGAACATCGTCAATAATTTCTTTGTGGAGCTAACTGAGACCTCTTTGTTGGCGGAAGATATTCGGAAGGTGGCGGATTTCATGCCAATTGAAAGATTTCACCACATTTCGAGGAAGGCGAACAGAACAGCTCACAGCTTGGCGAGGAAAGCATGCGAAGAGGGTTTATCGTTTGTGTGGTCGCTGAACTTCCCTTCGTGGCTCTCTAATTTGTTTCTTGTTGACAACGTTTAA

Protein sequence

MKRLCTFEDGIKVQVKSDCLEVVNIVNNFFVELTETSLLAEDIRKVADFMPIERFHHISRKANRTAHSLARKACEEGLSFVWSLNFPSWLSNLFLVDNV

Homology

[Close]

BLAST of Moc10g09390 vs. NCBI nr
Match: XP_022148549.1 (uncharacterized protein LOC111017181 [Momordica charantia])

HSP 1 Score: 80.5 bits (197), Expect = 9.0e-12
Identity = 38/69 (55.07%), Postives = 50/69 (72.46%), Query Frame = 0


Query: 6   TFEDGIKVQVKSDCLEVVNIVNNFFVELTETSLLAEDIRKVADFMPIERFHHISRKANRT 65
           T E GIK++++SDCLEVVNI+N   + LTE SL+ EDI K  + +PIE F H+  KAN  
Sbjct: 128 TLEAGIKIEMESDCLEVVNIINKSSMVLTEVSLIVEDIWKEMESLPIEGFKHLPMKANGV 187

Query: 66  AHSLARKAC 75
           AH +AR+AC
Sbjct: 188 AHGIARRAC 196

[Close]

BLAST of Moc10g09390 vs. NCBI nr
Match: XP_030483348.1 (uncharacterized protein LOC115699941 [Cannabis sativa])

HSP 1 Score: 56.2 bits (134), Expect = 1.8e-04
Identity = 29/83 (34.94%), Postives = 46/83 (55.42%), Query Frame = 0


Query: 15  VKSDCLEVVNIVNNFFVELTETSLLAEDIRKVADFMPIERFHHISRKANRTAHSLARKAC 74
           ++ DCL VVN +N+     T+ + L +DIR+  +F+      HISR  NR+AHSLA++A 
Sbjct: 283 IQIDCLRVVNYLNSISQAKTDWTALLDDIRETPEFVHCLAVQHISRDCNRSAHSLAKEAL 342

Query: 75  EEGLSFVWSLNFPSWLSNLFLVD 98
               + +W   +P   S   + D
Sbjct: 343 VSDCNKLWEGCYPFCASASLIAD 365

[Close]

BLAST of Moc10g09390 vs. NCBI nr
Match: XP_030945898.1 (uncharacterized protein LOC115970400 [Quercus lobata])

HSP 1 Score: 55.5 bits (132), Expect = 3.1e-04
Identity = 31/86 (36.05%), Postives = 45/86 (52.33%), Query Frame = 0


Query: 12  KVQVKSDCLEVVNIVNNFFVELTETSLLAEDIRKVADFMPIERFHHISRKANRTAHSLAR 71
           KV V+ DCL V+  +N     LT    +AE+ R++   + +  FHH+ R+ N+ AHSLAR
Sbjct: 89  KVMVEGDCLRVIQALNEQGSCLTMFGHVAEETRRLGSCLELCSFHHVKREGNKLAHSLAR 148

Query: 72  KACEEGLSFVWSLNFPSWLSNLFLVD 98
           +A       VW    P  L  +F  D
Sbjct: 149 RAVLPADVDVWVEELPEDLDAVFQGD 174

[Close]

BLAST of Moc10g09390 vs. NCBI nr
Match: XP_019150693.1 (PREDICTED: uncharacterized protein LOC109147547 [Ipomoea nil])

HSP 1 Score: 55.1 bits (131), Expect = 4.1e-04
Identity = 28/82 (34.15%), Postives = 45/82 (54.88%), Query Frame = 0


Query: 12  KVQVKSDCLEVVNIVNNFFVELTETSLLAEDIRKVADFMPIERFHHISRKANRTAHSLAR 71
           KV VK+DC  V N++N   ++L+    +  D R++     +  F ++SR  NR AH+LAR
Sbjct: 212 KVMVKTDCQAVCNLLNGSLLDLSFAGCVINDCRELKRHFDMVSFCYVSRSVNRLAHTLAR 271

Query: 72  KACEEGLSFVWSLNFPSWLSNL 94
            A  +     W   FPS +++L
Sbjct: 272 AARSDTDPHCWISTFPSCIASL 293

[Close]

BLAST of Moc10g09390 vs. NCBI nr
Match: XP_030943029.1 (uncharacterized protein LOC115967981 [Quercus lobata])

HSP 1 Score: 54.3 bits (129), Expect = 6.9e-04
Identity = 29/82 (35.37%), Postives = 46/82 (56.10%), Query Frame = 0


Query: 16  KSDCLEVVNIVNNFFVELTETSLLAEDIRKVADFMPIERFHHISRKANRTAHSLARKACE 75
           +SD L ++  +N   +   E   +  +IR+VA       F H+ R+ NR AH LA+ A  
Sbjct: 279 ESDALSIILAING-GIHGGELGHIIRNIREVAALFSWCYFKHLKREGNRVAHELAKVAGN 338

Query: 76  EGLSFVWSLNFPSWLSNLFLVD 98
            G+S VW ++FPS+L +L + D
Sbjct: 339 SGVSQVWKMSFPSFLEHLLIED 359

[Close]

BLAST of Moc10g09390 vs. ExPASy TrEMBL
Match: A0A6J1D4B6 (uncharacterized protein LOC111017181 OS=Momordica charantia OX=3673 GN=LOC111017181 PE=4 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 4.4e-12
Identity = 38/69 (55.07%), Postives = 50/69 (72.46%), Query Frame = 0


Query: 6   TFEDGIKVQVKSDCLEVVNIVNNFFVELTETSLLAEDIRKVADFMPIERFHHISRKANRT 65
           T E GIK++++SDCLEVVNI+N   + LTE SL+ EDI K  + +PIE F H+  KAN  
Sbjct: 128 TLEAGIKIEMESDCLEVVNIINKSSMVLTEVSLIVEDIWKEMESLPIEGFKHLPMKANGV 187

Query: 66  AHSLARKAC 75
           AH +AR+AC
Sbjct: 188 AHGIARRAC 196

[Close]

BLAST of Moc10g09390 vs. ExPASy TrEMBL
Match: A0A803NWY3 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 1.4e-05
Identity = 29/73 (39.73%), Postives = 43/73 (58.90%), Query Frame = 0


Query: 15   VKSDCLEVVNIVNNFFVELTETSLLAEDIRKVADFMPIERFHHISRKANRTAHSLARKAC 74
            +++DCL VVN +N      T+ S L +DIR+  +F+      HISR  NR+AHSLA++A 
Sbjct: 1585 IQTDCLRVVNYLNRISQATTDWSALLDDIRETPEFVHCLAVQHISRDCNRSAHSLAKEAL 1644

Query: 75   EEGLSFVWSLNFP 88
                + +W   FP
Sbjct: 1645 VSNCNKLWEGCFP 1657

[Close]

BLAST of Moc10g09390 vs. ExPASy TrEMBL
Match: A0A803QNS7 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 8.8e-05
Identity = 29/83 (34.94%), Postives = 46/83 (55.42%), Query Frame = 0


Query: 15  VKSDCLEVVNIVNNFFVELTETSLLAEDIRKVADFMPIERFHHISRKANRTAHSLARKAC 74
           ++ DCL VVN +N+     T+ + L +DIR+  +F+      HISR  NR+AHSLA++A 
Sbjct: 542 IQIDCLRVVNYLNSISQAKTDWTALLDDIRETPEFVHCLAVQHISRDCNRSAHSLAKEAL 601

Query: 75  EEGLSFVWSLNFPSWLSNLFLVD 98
               + +W   +P   S   + D
Sbjct: 602 VSDCNKLWEGCYPFCASASLIAD 624

[Close]

BLAST of Moc10g09390 vs. ExPASy TrEMBL
Match: A0A803PM23 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 8.8e-05
Identity = 29/83 (34.94%), Postives = 47/83 (56.63%), Query Frame = 0


Query: 15   VKSDCLEVVNIVNNFFVELTETSLLAEDIRKVADFMPIERFHHISRKANRTAHSLARKAC 74
            +++DCL VVN +N+     T+ S L +DIR+  +F+      HISR  NR+A+SLA++A 
Sbjct: 1286 IQTDCLRVVNYLNSISQAKTDWSALLDDIRETPEFVHCLAVQHISRDCNRSANSLAKEAL 1345

Query: 75   EEGLSFVWSLNFPSWLSNLFLVD 98
                + +W   +P   S   + D
Sbjct: 1346 ASDCNKLWEGCYPFCASASLIAD 1368

[Close]

BLAST of Moc10g09390 vs. ExPASy TrEMBL
Match: A0A7N2LIC5 (RNase H domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 2.6e-04
Identity = 30/86 (34.88%), Postives = 44/86 (51.16%), Query Frame = 0


Query: 12  KVQVKSDCLEVVNIVNNFFVELTETSLLAEDIRKVADFMPIERFHHISRKANRTAHSLAR 71
           KV V+ DCL V+  +N     LT    + E+ R++   + +  FHH+ R+ N+ AHSLAR
Sbjct: 84  KVMVEGDCLRVIQALNGQGSCLTMFGHVVEETRRLGSCLELCSFHHVKREGNKLAHSLAR 143

Query: 72  KACEEGLSFVWSLNFPSWLSNLFLVD 98
           +A       VW    P  L  +F  D
Sbjct: 144 RAVLSANVDVWVEELPEDLDAVFQGD 169

The following BLAST results are available for this feature:

BLAST of Moc10g09390 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022148549.1	9.0e-12	55.07	uncharacterized protein LOC111017181 [Momordica charantia]	[more]
XP_030483348.1	1.8e-04	34.94	uncharacterized protein LOC115699941 [Cannabis sativa]	[more]
XP_030945898.1	3.1e-04	36.05	uncharacterized protein LOC115970400 [Quercus lobata]	[more]
XP_019150693.1	4.1e-04	34.15	PREDICTED: uncharacterized protein LOC109147547 [Ipomoea nil]	[more]
XP_030943029.1	6.9e-04	35.37	uncharacterized protein LOC115967981 [Quercus lobata]	[more]

BLAST of Moc10g09390 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Moc10g09390 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D4B6	4.4e-12	55.07	uncharacterized protein LOC111017181 OS=Momordica charantia OX=3673 GN=LOC111017...	[more]
A0A803NWY3	1.4e-05	39.73	Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1	[more]
A0A803QNS7	8.8e-05	34.94	Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1	[more]
A0A803PM23	8.8e-05	34.94	Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1	[more]
A0A7N2LIC5	2.6e-04	34.88	RNase H domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1	[more]

BLAST of Moc10g09390 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 12..73 e-value: 5.1E-9 score: 36.0
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 2..81 e-value: 1.0E-5 score: 27.3

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc10g09390.1	Moc10g09390.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090304	nucleic acid metabolic process
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity