Moc10g08350 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc10g08350
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Phytocyanin domain-containing protein
Location	chr10: 6114285 .. 6115088 (-)
RNA-Seq Expression	Moc10g08350
Synteny	Moc10g08350

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGACAAAATGCATCTTACTTTGATGGTTGCCACCCTGAGCTGCCTAGTTTTGATGGAATCCGTGAATGCATTCACTCATATTGTCGGTGGGAACCACGGTTGGAGGGTGCCCGACAACAAAACCTTTTTTCAAGATTGGGCTAAACCCAGAACCTTTGGTGTCGGAGACAAACTTGGTACGTGTATACCTATCTGTTTGTCTTAAGCTTTTAATTCGAGTGGTAGTTTACAAGAGATCTTGCATTTGTTGGTTTGTTCATCACTAACCGTGTTTTTGTTGGTTTTCTTTGTGCAAGTTTTCCCGTACCGACCCGGCGCCAACAACTTAATGGCAGTGAAGAAGCCAGATTTCGACAAGTGCGAGCAAGATGACCCGATCAACATGTACTTCCAAGGCCCAACCATCTTGGAGCTCACCAAGCCTGGCGACTACTACTACTACGATGGCATTGGGAAGCACTGTGAAGCTGGCATGAAGCTCCACATTGTAGTTGGCACCAAAGAGGGAAGCTCTGGTGAGAATATGCCTTTCAAGCTTGACACTTTTGTCATTCGTACCTCCTCTCTCACTACCACCTCCGCCGCCACCGCCCCGGCCGCTGCCGCCTCTGGCCCCTCTGCCACCGTCTTCGCTGCTTCCTCTGGCCCCTCCGCCACCGTCTCCGCTGCCTCCACTGGCCCCTCGGCCTCTGCCTCCGCCACCGCCGCGGCTCCGAAGGCCTCCGCTGCCACTGCCACTCACAAGCTTGGCTTCGGAGTAATGTTGGTGGCCATGGCTTTGTCGATGTTCCTGCTCGTTTGA

mRNA sequence

ATGGACAAAATGCATCTTACTTTGATGGTTGCCACCCTGAGCTGCCTAGTTTTGATGGAATCCGTGAATGCATTCACTCATATTGTCGGTGGGAACCACGGTTGGAGGGTGCCCGACAACAAAACCTTTTTTCAAGATTGGGCTAAACCCAGAACCTTTGGTGTCGGAGACAAACTTGTTTTCCCGTACCGACCCGGCGCCAACAACTTAATGGCAGTGAAGAAGCCAGATTTCGACAAGTGCGAGCAAGATGACCCGATCAACATGTACTTCCAAGGCCCAACCATCTTGGAGCTCACCAAGCCTGGCGACTACTACTACTACGATGGCATTGGGAAGCACTGTGAAGCTGGCATGAAGCTCCACATTGTAGTTGGCACCAAAGAGGGAAGCTCTGGTGAGAATATGCCTTTCAAGCTTGACACTTTTGTCATTCGTACCTCCTCTCTCACTACCACCTCCGCCGCCACCGCCCCGGCCGCTGCCGCCTCTGGCCCCTCTGCCACCGTCTTCGCTGCTTCCTCTGGCCCCTCCGCCACCGTCTCCGCTGCCTCCACTGGCCCCTCGGCCTCTGCCTCCGCCACCGCCGCGGCTCCGAAGGCCTCCGCTGCCACTGCCACTCACAAGCTTGGCTTCGGAGTAATGTTGGTGGCCATGGCTTTGTCGATGTTCCTGCTCGTTTGA

Coding sequence (CDS)

ATGGACAAAATGCATCTTACTTTGATGGTTGCCACCCTGAGCTGCCTAGTTTTGATGGAATCCGTGAATGCATTCACTCATATTGTCGGTGGGAACCACGGTTGGAGGGTGCCCGACAACAAAACCTTTTTTCAAGATTGGGCTAAACCCAGAACCTTTGGTGTCGGAGACAAACTTGTTTTCCCGTACCGACCCGGCGCCAACAACTTAATGGCAGTGAAGAAGCCAGATTTCGACAAGTGCGAGCAAGATGACCCGATCAACATGTACTTCCAAGGCCCAACCATCTTGGAGCTCACCAAGCCTGGCGACTACTACTACTACGATGGCATTGGGAAGCACTGTGAAGCTGGCATGAAGCTCCACATTGTAGTTGGCACCAAAGAGGGAAGCTCTGGTGAGAATATGCCTTTCAAGCTTGACACTTTTGTCATTCGTACCTCCTCTCTCACTACCACCTCCGCCGCCACCGCCCCGGCCGCTGCCGCCTCTGGCCCCTCTGCCACCGTCTTCGCTGCTTCCTCTGGCCCCTCCGCCACCGTCTCCGCTGCCTCCACTGGCCCCTCGGCCTCTGCCTCCGCCACCGCCGCGGCTCCGAAGGCCTCCGCTGCCACTGCCACTCACAAGCTTGGCTTCGGAGTAATGTTGGTGGCCATGGCTTTGTCGATGTTCCTGCTCGTTTGA

Protein sequence

MDKMHLTLMVATLSCLVLMESVNAFTHIVGGNHGWRVPDNKTFFQDWAKPRTFGVGDKLVFPYRPGANNLMAVKKPDFDKCEQDDPINMYFQGPTILELTKPGDYYYYDGIGKHCEAGMKLHIVVGTKEGSSGENMPFKLDTFVIRTSSLTTTSAATAPAAAASGPSATVFAASSGPSATVSAASTGPSASASATAAAPKASAATATHKLGFGVMLVAMALSMFLLV

Homology

[Close]

BLAST of Moc10g08350 vs. NCBI nr
Match: XP_022136659.1 (blue copper protein-like [Momordica charantia])

HSP 1 Score: 431.0 bits (1107), Expect = 6.3e-117
Identity = 227/227 (100.00%), Postives = 227/227 (100.00%), Query Frame = 0


Query: 1   MDKMHLTLMVATLSCLVLMESVNAFTHIVGGNHGWRVPDNKTFFQDWAKPRTFGVGDKLV 60
           MDKMHLTLMVATLSCLVLMESVNAFTHIVGGNHGWRVPDNKTFFQDWAKPRTFGVGDKLV
Sbjct: 1   MDKMHLTLMVATLSCLVLMESVNAFTHIVGGNHGWRVPDNKTFFQDWAKPRTFGVGDKLV 60

Query: 61  FPYRPGANNLMAVKKPDFDKCEQDDPINMYFQGPTILELTKPGDYYYYDGIGKHCEAGMK 120
           FPYRPGANNLMAVKKPDFDKCEQDDPINMYFQGPTILELTKPGDYYYYDGIGKHCEAGMK
Sbjct: 61  FPYRPGANNLMAVKKPDFDKCEQDDPINMYFQGPTILELTKPGDYYYYDGIGKHCEAGMK 120

Query: 121 LHIVVGTKEGSSGENMPFKLDTFVIRTSSLTTTSAATAPAAAASGPSATVFAASSGPSAT 180
           LHIVVGTKEGSSGENMPFKLDTFVIRTSSLTTTSAATAPAAAASGPSATVFAASSGPSAT
Sbjct: 121 LHIVVGTKEGSSGENMPFKLDTFVIRTSSLTTTSAATAPAAAASGPSATVFAASSGPSAT 180

Query: 181 VSAASTGPSASASATAAAPKASAATATHKLGFGVMLVAMALSMFLLV 228
           VSAASTGPSASASATAAAPKASAATATHKLGFGVMLVAMALSMFLLV
Sbjct: 181 VSAASTGPSASASATAAAPKASAATATHKLGFGVMLVAMALSMFLLV 227

[Close]

BLAST of Moc10g08350 vs. NCBI nr
Match: XP_022982873.1 (umecyanin-like [Cucurbita maxima])

HSP 1 Score: 231.5 bits (589), Expect = 7.3e-57
Identity = 122/189 (64.55%), Postives = 141/189 (74.60%), Query Frame = 0


Query: 1   MDKMHLTLMVATLSCLVLMESVNAFTHIVGGNHGWRVPDNKTFFQDWAKPRTFGVGDKLV 60
           MDKM+L LM+  LSCLV M S++AFTHIVGGNHGWRVPDN TFF +WAKPRTFGVGDKLV
Sbjct: 1   MDKMYLALMIGILSCLVFMGSMDAFTHIVGGNHGWRVPDNVTFFDEWAKPRTFGVGDKLV 60

Query: 61  FPYRPGANNLMAVKKPDFDKCEQ--DDPINMYFQGPTILELTKPGDYYYYDGIGKHCEAG 120
           FPYRPGANNL+AVKK D++ C Q  +D INMY+ GPTIL +T PGDYYYYDGIGKHCEAG
Sbjct: 61  FPYRPGANNLLAVKKADYETCGQGEEDVINMYYLGPTILNITHPGDYYYYDGIGKHCEAG 120

Query: 121 MKLHIVVGTKEGSSGEN-MPFKLDTFVIRTSSLTTTS--AATAPAAAASGPSATVFAASS 180
            KLH+ VG+KEGSSG + +PF L TF I T+  TT    AA      A+ P  T  AA +
Sbjct: 121 QKLHVQVGSKEGSSGSDPLPFNLQTFGIPTAFGTTVEPPAAAPVVTPAAAPVVTPAAAMA 180

Query: 181 GPSATVSAA 185
                 S+A
Sbjct: 181 HKFGLASSA 189

[Close]

BLAST of Moc10g08350 vs. NCBI nr
Match: XP_023518646.1 (umecyanin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 230.7 bits (587), Expect = 1.2e-56
Identity = 115/178 (64.61%), Postives = 136/178 (76.40%), Query Frame = 0


Query: 1   MDKMHLTLMVATLSCLVLMESVNAFTHIVGGNHGWRVPDNKTFFQDWAKPRTFGVGDKLV 60
           MDKM++ LM+   SCL+ M S++AFTHIVGGNHGWRVPDN TFF +WAKPRTFGVGDKLV
Sbjct: 1   MDKMYVALMIGIFSCLLFMPSMDAFTHIVGGNHGWRVPDNVTFFDEWAKPRTFGVGDKLV 60

Query: 61  FPYRPGANNLMAVKKPDFDKCEQ--DDPINMYFQGPTILELTKPGDYYYYDGIGKHCEAG 120
           FPYRPGANNL+AVKK D++ C Q  +D INMY+ GPTIL +T+PGDYYYYDGIGKHCEAG
Sbjct: 61  FPYRPGANNLLAVKKADYETCGQGEEDVINMYYLGPTILNITQPGDYYYYDGIGKHCEAG 120

Query: 121 MKLHIVVGTKEGSSGEN-MPFKLDTFVIRTSSLTTTS-AATAPAAAASGPSATVFAAS 175
            KLHI VG+KEG+SG + +PF L TF I T+  T     A AP    + P     AA+
Sbjct: 121 QKLHIQVGSKEGTSGSDPLPFNLQTFGIPTAFGTMVEPPAAAPVVTLAAPRVVTPAAA 178

[Close]

BLAST of Moc10g08350 vs. NCBI nr
Match: XP_022942920.1 (stellacyanin-like [Cucurbita moschata] >KAG7031173.1 Early nodulin-like protein 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 227.6 bits (579), Expect = 1.1e-55
Identity = 125/195 (64.10%), Postives = 144/195 (73.85%), Query Frame = 0


Query: 1   MDKMHLTLMVATLSCLVLMESVNAFTHIVGGNHGWRVPDNKTFFQDWAKPRTFGVGDKLV 60
           MDKM++ LM+  LSCLV M S++AFTHIVGGNHGWRVPDN TFF +WAKPRTFGVGDKLV
Sbjct: 1   MDKMYVALMIGILSCLVFMPSMDAFTHIVGGNHGWRVPDNVTFFDEWAKPRTFGVGDKLV 60

Query: 61  FPYRPGANNLMAVKKPDFDKCEQ--DDPINMYFQGPTILELTKPGDYYYYDGIGKHCEAG 120
           FPYRPGANNL+AVKK D++ C Q  +D INMY+ GPTIL +T PGDYYYYDGIGKHCEAG
Sbjct: 61  FPYRPGANNLLAVKKADYETCGQGEEDVINMYYLGPTILNITHPGDYYYYDGIGKHCEAG 120

Query: 121 MKLHIVVGTKEGSSGEN-MPFKLDTFVIRTSSLTTTSAATAPAAAASGPSATVFAASSGP 180
            KLHI VG KEG+SG + +PF L TF I T+  TT      P AAA  P  T+ AA    
Sbjct: 121 QKLHIQVGLKEGTSGSDPLPFNLQTFGIPTAFGTTVE----PPAAA--PVVTLDAARVVT 180

Query: 181 SATVSAASTGPSASA 193
            A   A   G ++SA
Sbjct: 181 PAAAMAHKFGLASSA 189

[Close]

BLAST of Moc10g08350 vs. NCBI nr
Match: XP_038878156.1 (stellacyanin-like [Benincasa hispida])

HSP 1 Score: 215.7 bits (548), Expect = 4.1e-52
Identity = 105/167 (62.87%), Postives = 129/167 (77.25%), Query Frame = 0


Query: 4   MHLTLMVATLSCLVLMESVNAFTHIVGGNHGWRVPDNKTFFQDWAKPRTFGVGDKLVFPY 63
           M  T+M+  L  +++   VNAFTHIVGG+HGWRVP+N TFF +W KPRTFGVGD+LVFPY
Sbjct: 1   MDKTVMICCLVMMMMGSCVNAFTHIVGGSHGWRVPENITFFDEWTKPRTFGVGDRLVFPY 60

Query: 64  RPGANNLMAVKKPDFDKCEQDDPINMYFQGPTILELTKPGDYYYYDGIGKHCEAGMKLHI 123
           RPGANNL+AVKK D+D C ++  I+MYF GPT+L LT+PGDYYY+DGIGKHCEAG KLHI
Sbjct: 61  RPGANNLLAVKKADYDTCGEEAVIHMYFLGPTVLNLTEPGDYYYFDGIGKHCEAGQKLHI 120

Query: 124 VVGTKEGSSGEN-MPFKLDTFVIRTS--SLTTTSAATAPAAAASGPS 168
            VG+KEG+SG + MPF L+TF I T+       +A T  AA A+ PS
Sbjct: 121 QVGSKEGTSGSDPMPFNLETFGIHTNLGPAMAPTAQTQAAANATHPS 167

[Close]

BLAST of Moc10g08350 vs. ExPASy Swiss-Prot
Match: P42849 (Umecyanin OS=Armoracia rusticana OX=3704 PE=1 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 4.2e-15
Identity = 39/109 (35.78%), Postives = 57/109 (52.29%), Query Frame = 0


Query: 29  VGGNHGWRVPDNKTFFQDWAKPRTFGVGDKLVFPYRPGANNLMAVKKPDFDKCEQDDPIN 88
           VGG+  W+ P +  F+  WA  +TF VGD+L F +  G +++  V K  FD C++++PI+
Sbjct: 5   VGGDMEWKRPSDPKFYITWATGKTFRVGDELEFDFAAGMHDVAVVTKDAFDNCKKENPIS 64

Query: 89  MYFQGPTILELTKPGDYYYYDGIGKHCEAGMKLHIVVGTKEGSSGENMP 138
                P  + L   G  YY   +G HC  G KL I V    G+ G   P
Sbjct: 65  HMTTPPVKIMLNTTGPQYYICTVGDHCRVGQKLSINVVGAGGAGGGATP 113

[Close]

BLAST of Moc10g08350 vs. ExPASy Swiss-Prot
Match: P00302 (Stellacyanin OS=Toxicodendron vernicifluum OX=4013 PE=1 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 1.5e-12
Identity = 39/105 (37.14%), Postives = 53/105 (50.48%), Query Frame = 0


Query: 27  HIVGGNHGWRVPDNKTFFQD------WAKPRTFGVGDKLVFPYRPGANNLMAVKKPDFDK 86
           + VG + GW+VP    FF D      WA  +TF +GD LVF Y    +N+  V + ++  
Sbjct: 3   YTVGDSAGWKVP----FFGDVDYDWKWASNKTFHIGDVLVFKYDRRFHNVDKVTQKNYQS 62

Query: 87  CEQDDPINMYFQGPTILELTKPGDYYYYDGIGKHCEAGMKLHIVV 126
           C    PI  Y  G   + L   G  YY  G+ KHC+ G K+HI V
Sbjct: 63  CNDTTPIASYNTGBBRINLKTVGQKYYICGVPKHCDLGQKVHINV 103

[Close]

BLAST of Moc10g08350 vs. ExPASy Swiss-Prot
Match: Q41001 (Blue copper protein OS=Pisum sativum OX=3888 PE=2 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 1.9e-12
Identity = 52/161 (32.30%), Postives = 72/161 (44.72%), Query Frame = 0


Query: 24  AFTHIVGGNHGWRVPDNKTFFQDWAKPRTFGVGDKLVFPYRPGANNLMAVKKPDFDKCEQ 83
           A  + VG   GW +  +   +  WA  +TF VGD LVF Y  GA+ +  VK+ D+  C  
Sbjct: 24  ATVYTVGDTSGWVIGGD---YSTWASDKTFAVGDSLVFNYGAGAHTVDEVKESDYKSCTS 83

Query: 84  DDPINMYFQGPTILELTKPGDYYYYDGIGKHCEAGMKLHIVVGTKEG----------SSG 143
            + I+    G T + L K G +Y+  G+  H   GMKL I V    G          SSG
Sbjct: 84  GNSISTDSTGATTIPLKKAGKHYFICGVPGHSTGGMKLSIKVKASSGSSAAPSATPSSSG 143

Query: 144 ENMPFKLDTFVIRTSSLTTTSAATAPAAAASGPSATVFAAS 175
           +  P   DT    T++ T T    + A + S   A  F  S
Sbjct: 144 KGSPSSDDTPAATTTTTTPTKQNESSATSLSPIVALFFTVS 181

[Close]

BLAST of Moc10g08350 vs. ExPASy Swiss-Prot
Match: P29602 (Cucumber peeling cupredoxin OS=Cucumis sativus OX=3659 PE=1 SV=3)

HSP 1 Score: 73.6 bits (179), Expect = 3.3e-12
Identity = 39/101 (38.61%), Postives = 53/101 (52.48%), Query Frame = 0


Query: 27  HIVGGNHGWRVPDNKTFFQDWAKPRTFGVGDKLVFPYRPGANNLMAVK-KPDFDKCEQDD 86
           HIVG N GW VP +  F+  WA  +TF VGD L F +   A+N+  ++ K  FD C   +
Sbjct: 5   HIVGDNTGWSVPSSPNFYSQWAAGKTFRVGDSLQFNFPANAHNVHEMETKQSFDACNFVN 64

Query: 87  PIN-MYFQGPTILELTKPGDYYYYDGIGKHCEAGMKLHIVV 126
             N +    P I  L + G +Y+   +G HC  G KL I V
Sbjct: 65  SDNDVERTSPVIERLDELGMHYFVCTVGTHCSNGQKLSINV 105

[Close]

BLAST of Moc10g08350 vs. ExPASy Swiss-Prot
Match: P80728 (Mavicyanin OS=Cucurbita pepo OX=3663 PE=1 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 1.7e-11
Identity = 37/111 (33.33%), Postives = 52/111 (46.85%), Query Frame = 0


Query: 24  AFTHIVGGNHGWR--VPDNKTFFQDWAKPRTFGVGDKLVFPYRPGANNLMAVKKPDFDKC 83
           A  H VG + GW   VP +   +  WA    F VGD L+F Y    +N++ V +  F  C
Sbjct: 1   ATVHKVGDSTGWTTLVPYD---YAKWASSNKFHVGDSLLFNYNNKFHNVLQVDQEQFKSC 60

Query: 84  EQDDPINMYFQGPTILELTKPGDYYYYDGIGKHCEAGMKLHIVVGTKEGSS 133
               P   Y  G   + L +PG +Y+  GI  HC+ G K+ I V     S+
Sbjct: 61  NSSSPAASYTSGADSIPLKRPGTFYFLCGIPGHCQLGQKVEIKVDPGSSSA 108

[Close]

BLAST of Moc10g08350 vs. ExPASy TrEMBL
Match: A0A6J1C4Y4 (blue copper protein-like OS=Momordica charantia OX=3673 GN=LOC111008312 PE=4 SV=1)

HSP 1 Score: 431.0 bits (1107), Expect = 3.0e-117
Identity = 227/227 (100.00%), Postives = 227/227 (100.00%), Query Frame = 0


Query: 1   MDKMHLTLMVATLSCLVLMESVNAFTHIVGGNHGWRVPDNKTFFQDWAKPRTFGVGDKLV 60
           MDKMHLTLMVATLSCLVLMESVNAFTHIVGGNHGWRVPDNKTFFQDWAKPRTFGVGDKLV
Sbjct: 1   MDKMHLTLMVATLSCLVLMESVNAFTHIVGGNHGWRVPDNKTFFQDWAKPRTFGVGDKLV 60

Query: 61  FPYRPGANNLMAVKKPDFDKCEQDDPINMYFQGPTILELTKPGDYYYYDGIGKHCEAGMK 120
           FPYRPGANNLMAVKKPDFDKCEQDDPINMYFQGPTILELTKPGDYYYYDGIGKHCEAGMK
Sbjct: 61  FPYRPGANNLMAVKKPDFDKCEQDDPINMYFQGPTILELTKPGDYYYYDGIGKHCEAGMK 120

Query: 121 LHIVVGTKEGSSGENMPFKLDTFVIRTSSLTTTSAATAPAAAASGPSATVFAASSGPSAT 180
           LHIVVGTKEGSSGENMPFKLDTFVIRTSSLTTTSAATAPAAAASGPSATVFAASSGPSAT
Sbjct: 121 LHIVVGTKEGSSGENMPFKLDTFVIRTSSLTTTSAATAPAAAASGPSATVFAASSGPSAT 180

Query: 181 VSAASTGPSASASATAAAPKASAATATHKLGFGVMLVAMALSMFLLV 228
           VSAASTGPSASASATAAAPKASAATATHKLGFGVMLVAMALSMFLLV
Sbjct: 181 VSAASTGPSASASATAAAPKASAATATHKLGFGVMLVAMALSMFLLV 227

[Close]

BLAST of Moc10g08350 vs. ExPASy TrEMBL
Match: A0A6J1J638 (umecyanin-like OS=Cucurbita maxima OX=3661 GN=LOC111481590 PE=4 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 3.5e-57
Identity = 122/189 (64.55%), Postives = 141/189 (74.60%), Query Frame = 0


Query: 1   MDKMHLTLMVATLSCLVLMESVNAFTHIVGGNHGWRVPDNKTFFQDWAKPRTFGVGDKLV 60
           MDKM+L LM+  LSCLV M S++AFTHIVGGNHGWRVPDN TFF +WAKPRTFGVGDKLV
Sbjct: 1   MDKMYLALMIGILSCLVFMGSMDAFTHIVGGNHGWRVPDNVTFFDEWAKPRTFGVGDKLV 60

Query: 61  FPYRPGANNLMAVKKPDFDKCEQ--DDPINMYFQGPTILELTKPGDYYYYDGIGKHCEAG 120
           FPYRPGANNL+AVKK D++ C Q  +D INMY+ GPTIL +T PGDYYYYDGIGKHCEAG
Sbjct: 61  FPYRPGANNLLAVKKADYETCGQGEEDVINMYYLGPTILNITHPGDYYYYDGIGKHCEAG 120

Query: 121 MKLHIVVGTKEGSSGEN-MPFKLDTFVIRTSSLTTTS--AATAPAAAASGPSATVFAASS 180
            KLH+ VG+KEGSSG + +PF L TF I T+  TT    AA      A+ P  T  AA +
Sbjct: 121 QKLHVQVGSKEGSSGSDPLPFNLQTFGIPTAFGTTVEPPAAAPVVTPAAAPVVTPAAAMA 180

Query: 181 GPSATVSAA 185
                 S+A
Sbjct: 181 HKFGLASSA 189

[Close]

BLAST of Moc10g08350 vs. ExPASy TrEMBL
Match: A0A6J1FX52 (stellacyanin-like OS=Cucurbita moschata OX=3662 GN=LOC111447806 PE=4 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 5.1e-56
Identity = 125/195 (64.10%), Postives = 144/195 (73.85%), Query Frame = 0


Query: 1   MDKMHLTLMVATLSCLVLMESVNAFTHIVGGNHGWRVPDNKTFFQDWAKPRTFGVGDKLV 60
           MDKM++ LM+  LSCLV M S++AFTHIVGGNHGWRVPDN TFF +WAKPRTFGVGDKLV
Sbjct: 1   MDKMYVALMIGILSCLVFMPSMDAFTHIVGGNHGWRVPDNVTFFDEWAKPRTFGVGDKLV 60

Query: 61  FPYRPGANNLMAVKKPDFDKCEQ--DDPINMYFQGPTILELTKPGDYYYYDGIGKHCEAG 120
           FPYRPGANNL+AVKK D++ C Q  +D INMY+ GPTIL +T PGDYYYYDGIGKHCEAG
Sbjct: 61  FPYRPGANNLLAVKKADYETCGQGEEDVINMYYLGPTILNITHPGDYYYYDGIGKHCEAG 120

Query: 121 MKLHIVVGTKEGSSGEN-MPFKLDTFVIRTSSLTTTSAATAPAAAASGPSATVFAASSGP 180
            KLHI VG KEG+SG + +PF L TF I T+  TT      P AAA  P  T+ AA    
Sbjct: 121 QKLHIQVGLKEGTSGSDPLPFNLQTFGIPTAFGTTVE----PPAAA--PVVTLDAARVVT 180

Query: 181 SATVSAASTGPSASA 193
            A   A   G ++SA
Sbjct: 181 PAAAMAHKFGLASSA 189

[Close]

BLAST of Moc10g08350 vs. ExPASy TrEMBL
Match: A0A6J1J846 (stellacyanin-like OS=Cucurbita maxima OX=3661 GN=LOC111483431 PE=4 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 3.4e-52
Identity = 107/175 (61.14%), Postives = 134/175 (76.57%), Query Frame = 0


Query: 1   MDKMHLTLMVATLSCLVLMESVNAFTHIVGGNHGWRVPDNKTFFQDWAKPRTFGVGDKLV 60
           MDK  + +M  T   +++  SVNAFTHIVG +HGWRVPDN TF+ +WAKPRTFGVGDKLV
Sbjct: 1   MDKF-MMIMTITTMMMMMGSSVNAFTHIVGASHGWRVPDNVTFYDEWAKPRTFGVGDKLV 60

Query: 61  FPYRPGANNLMAVKKPDFDKCEQDDPINMYFQGPTILELTKPGDYYYYDGIGKHCEAGMK 120
           FPYRPGANN++AVKK D++ C +++ INMY+ GPTIL LT+ GDYYY+DGIGKHCEAG K
Sbjct: 61  FPYRPGANNIVAVKKTDYEVCGEENVINMYYLGPTILNLTEAGDYYYFDGIGKHCEAGQK 120

Query: 121 LHIVVGTKEGSSG-ENMPFKLDTFVIRTSSLTTTSA--ATAPAAAASGPSATVFA 173
           LH+ VG+KEG+SG + +PF L+TF I T+   T +   A AP AA  G +A V A
Sbjct: 121 LHVQVGSKEGTSGADPLPFNLETFGIHTNLDPTQNVGPAAAPTAATKGSNANVNA 174

[Close]

BLAST of Moc10g08350 vs. ExPASy TrEMBL
Match: A0A6J1ETX6 (stellacyanin-like OS=Cucurbita moschata OX=3662 GN=LOC111437535 PE=4 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 2.3e-48
Identity = 108/184 (58.70%), Postives = 136/184 (73.91%), Query Frame = 0


Query: 8   LMVATLSCLVLMESVNAFTHIVGGNHGWRVPDNKTFFQDWAKPRTFGVGDKLVFPYRPGA 67
           +M+ T+  +++  SVNAFTHIVG +HGWRVPDN TF+ +WAKPRTFGVGDKLVFPYRPGA
Sbjct: 5   MMIMTI-MMMMGSSVNAFTHIVGASHGWRVPDNVTFYDEWAKPRTFGVGDKLVFPYRPGA 64

Query: 68  NNLMAVKKPDFDKCEQDDPINMYFQGPTILELTKPGDYYYYDGIGKHCEAGMKLHIVVGT 127
           NN++AVKK D++ C +++ INMY+ GPTIL LT+ GDYYY+DGIGKHCEAG KLH+ VG 
Sbjct: 65  NNIVAVKKADYEVCGEENVINMYYLGPTILNLTEAGDYYYFDGIGKHCEAGQKLHVQVGL 124

Query: 128 KEGSSG-ENMPFKLDTFVIRTSSLTT--TSAATAPAAA--ASGPSATVFAASSGPSATVS 187
           KEG+SG + +PF L+TF I TS   T   + A AP AA  AS  +A      +G +A   
Sbjct: 125 KEGTSGADPLPFNLETFGIHTSLDPTQNVAPAAAPTAATKASHANANALLVPAGLAAVAM 184

[Close]

BLAST of Moc10g08350 vs. TAIR 10
Match: AT5G26330.1 (Cupredoxin superfamily protein )

HSP 1 Score: 81.3 bits (199), Expect = 1.1e-15
Identity = 53/167 (31.74%), Postives = 88/167 (52.69%), Query Frame = 0


Query: 8   LMVATLSCLVLMESVN-AFTHIVGGNHGWRVPDNKTFFQDWAKPRTFGVGDKLVFPYRPG 67
           ++VA L+C+V+M  ++ A  + VG + GW    N   ++ WA  +TF +GD ++F Y P 
Sbjct: 4   IIVAALACIVVMLRLSEAAVYKVGDSAGWTTIAN-VDYKLWASTKTFHIGDTVLFEYNPQ 63

Query: 68  ANNLMAVKKPDFDKCEQDDPINMYFQGPTILELTKPGDYYYYDGIGKHCEAGMKLHIVVG 127
            +N+M V  P +  C    PI+ +  G   + LT  G ++++ G+  HC AG KL + V 
Sbjct: 64  FHNVMRVTHPMYRSCNTSKPISTFTTGNDSITLTNHGHHFFFCGVPGHCLAGQKLDLHVL 123

Query: 128 TKEGSSGENMPFKLDTFVIRTSSLTTTSAATAPAAAASGPSATVFAA 174
               S+  + P         TSS ++  + T PAA   GPS ++ A+
Sbjct: 124 LPASSTPLSDP--------PTSSSSSPPSTTIPAAGVPGPSPSLAAS 161

[Close]

BLAST of Moc10g08350 vs. TAIR 10
Match: AT3G18590.1 (early nodulin-like protein 5 )

HSP 1 Score: 75.1 bits (183), Expect = 8.1e-14
Identity = 49/174 (28.16%), Postives = 82/174 (47.13%), Query Frame = 0


Query: 1   MDKMHLTLMVATLSCLVLMESVNAFTHIVGGNHGWRVPDNKTF---FQDWAKPRTFGVGD 60
           MD     ++V  L    +   V++    VGG +GW VP +KT    F  WA    F VGD
Sbjct: 1   MDSSKKIIIVMFLVTFYMFSCVSSTEFEVGGENGWIVPKSKTLGDAFNQWASDNRFKVGD 60

Query: 61  KLVFPYRPGANNLMAVKKPDFDKCEQDDPINMYFQGPTILELTKPGDYYYYDGIGKHCEA 120
            L F Y    ++++ V + ++ KC+   P        T+ +L +PG +Y+  G+  HCE 
Sbjct: 61  TLRFKYT--KDSVLVVSEEEYKKCKATKPQLYSNNEDTVFKLDRPGLFYFISGVSGHCEK 120

Query: 121 GMKLHIVVGTKEGSSGENMPFKLDTFVIRTSSLTTTSAATAPAAAASGPSATVF 172
           G K+ + V   E S+    P         +SS +++S+++ PA+      +  F
Sbjct: 121 GQKMIVKVMETESSTESPPP---------SSSSSSSSSSSLPASTPKAKKSNAF 163

[Close]

BLAST of Moc10g08350 vs. TAIR 10
Match: AT2G32300.1 (uclacyanin 1 )

HSP 1 Score: 70.5 bits (171), Expect = 2.0e-12
Identity = 76/259 (29.34%), Postives = 118/259 (45.56%), Query Frame = 0


Query: 8   LMVATLSCLVLMESVNAFTHIVGGNHGWRVPDNKTFFQDWAKPRTFGVGDKLVFPYRPGA 67
           L++ ++    L+    A  H +GG  GW V  +    + WA  +TF VGD LVF Y    
Sbjct: 7   LIIISVLATTLIGLTVATDHTIGGPSGWTVGAS---LRTWAAGQTFAVGDNLVFSYPAAF 66

Query: 68  NNLMAVKKPDFDKCEQDDPINMYFQGPTILELTKPGDYYYYDGIGKHCEAGMKLHI-VVG 127
           ++++ V KP+FD C+   P+  +  G +++ LT PG  Y+  G+  HC  GMKL + VV 
Sbjct: 67  HDVVEVTKPEFDSCQAVKPLITFANGNSLVPLTTPGKRYFICGMPGHCSQGMKLEVNVVP 126

Query: 128 TKEGSSGENMPFKLDTF---------------------VIRTSSLTTTSAATAP------ 187
           T   +    +P  + +                      V+  SS T   +++ P      
Sbjct: 127 TATVAPTAPLPNTVPSLNAPSPSSVLPIQPLLPLNPVPVLSPSSSTPLPSSSLPLIPPLS 186

Query: 188 ----AAAASGPSATVFAASSGPSA---------TVSAASTGPSAS-ASATAAAPKASAAT 225
                A A+G S  +F  S G S+         T  +++TG +A  A A +    +S+A 
Sbjct: 187 PALSPATAAGTSLPLFPGSPGSSSSTTSTKTVGTFPSSTTGTTADLAGADSPPADSSSAA 246

[Close]

BLAST of Moc10g08350 vs. TAIR 10
Match: AT3G20570.1 (early nodulin-like protein 9 )

HSP 1 Score: 65.1 bits (157), Expect = 8.4e-11
Identity = 54/201 (26.87%), Postives = 88/201 (43.78%), Query Frame = 0


Query: 13  LSCLVLMESVNAFTHIVGGNHGWRVPDNKTFFQDWAKPRTFGVGDKLVFPYRPGANNLMA 72
           L  L++++   A    VGG  GW VP     +  WA+   F +GD L+F Y+   ++++ 
Sbjct: 16  LCFLMIVDRAYAREFTVGGATGWTVPSGSQVYSQWAEQSRFQIGDSLLFVYQSNQDSVLQ 75

Query: 73  VKKPDFDKCEQDDPINMYFQGPTILELTKPGDYYYYDGIGKHCEAGMKLHIVVGTKEGSS 132
           V +  +D C  D P   +  G T + L   G YY+  G   +C+   KL ++V       
Sbjct: 76  VTRDAYDSCNTDSPTAKFADGKTSVTLNHSGPYYFISGNKDNCKKNEKLVVIV------- 135

Query: 133 GENMPFKLDTFVIRTSSLTTTSAATAPAAAASGPSAT------VFAASSGPSATVSAAST 192
                        R+ +  T S+  +PA A SG SA        F  +  P+ T S  + 
Sbjct: 136 ----------MADRSGNKNTASSPPSPAPAPSGESAPSPPVSGTFEMTPAPTPTTSEDTP 195

Query: 193 GPSASASATAAAPKASAATAT 208
             +AS+ +  AA   +A  +T
Sbjct: 196 NSAASSLSFVAALLGAALAST 199

[Close]

BLAST of Moc10g08350 vs. TAIR 10
Match: AT5G53870.1 (early nodulin-like protein 1 )

HSP 1 Score: 62.8 bits (151), Expect = 4.2e-10
Identity = 55/185 (29.73%), Postives = 86/185 (46.49%), Query Frame = 0


Query: 23  NAFTHIVGGNHGWRVPDNKTFFQDWAKPRTFGVGDKLVFPYRPGANNLMAVKKPDFDKCE 82
           +A+   VGGN  W V + +  +  WA+   F V D L F Y  G++++  V K DFD C 
Sbjct: 26  DAWRFNVGGNGAW-VTNPQENYNTWAERNRFQVNDSLYFKYAKGSDSVQQVMKADFDGCN 85

Query: 83  QDDPINMYFQGPTILELTKPGDYYYYDGIGKHCEAGMKLHIVVGTKEGSSGENMPFKLDT 142
             +PI  +  G +++ L + G +Y+  G   HC+ G KL +VV         + P     
Sbjct: 86  VRNPIKNFENGESVVTLDRSGAFYFISGNQDHCQKGQKLIVVVLAVRNQ--PSAPAHSPV 145

Query: 143 FVIRTSSLTTTSAATAPAAAASGPSATVFAASSGPSATVSAASTGPSASASATAAAPKAS 202
             +  +    + +  +P A AS PS      S  P ++VS A   P  S+S  +  P  S
Sbjct: 146 PSVSPTQPPKSHSPVSPVAPASAPS-----KSQPPRSSVSPAQ--PPKSSSPISHTPALS 200

Query: 203 AATAT 208
            + AT
Sbjct: 206 PSHAT 200

The following BLAST results are available for this feature:

BLAST of Moc10g08350 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022136659.1	6.3e-117	100.00	blue copper protein-like [Momordica charantia]	[more]
XP_022982873.1	7.3e-57	64.55	umecyanin-like [Cucurbita maxima]	[more]
XP_023518646.1	1.2e-56	64.61	umecyanin-like [Cucurbita pepo subsp. pepo]	[more]
XP_022942920.1	1.1e-55	64.10	stellacyanin-like [Cucurbita moschata] >KAG7031173.1 Early nodulin-like protein ...	[more]
XP_038878156.1	4.1e-52	62.87	stellacyanin-like [Benincasa hispida]	[more]

BLAST of Moc10g08350 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P42849	4.2e-15	35.78	Umecyanin OS=Armoracia rusticana OX=3704 PE=1 SV=1	[more]
P00302	1.5e-12	37.14	Stellacyanin OS=Toxicodendron vernicifluum OX=4013 PE=1 SV=1	[more]
Q41001	1.9e-12	32.30	Blue copper protein OS=Pisum sativum OX=3888 PE=2 SV=1	[more]
P29602	3.3e-12	38.61	Cucumber peeling cupredoxin OS=Cucumis sativus OX=3659 PE=1 SV=3	[more]
P80728	1.7e-11	33.33	Mavicyanin OS=Cucurbita pepo OX=3663 PE=1 SV=1	[more]

BLAST of Moc10g08350 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C4Y4	3.0e-117	100.00	blue copper protein-like OS=Momordica charantia OX=3673 GN=LOC111008312 PE=4 SV=...	[more]
A0A6J1J638	3.5e-57	64.55	umecyanin-like OS=Cucurbita maxima OX=3661 GN=LOC111481590 PE=4 SV=1	[more]
A0A6J1FX52	5.1e-56	64.10	stellacyanin-like OS=Cucurbita moschata OX=3662 GN=LOC111447806 PE=4 SV=1	[more]
A0A6J1J846	3.4e-52	61.14	stellacyanin-like OS=Cucurbita maxima OX=3661 GN=LOC111483431 PE=4 SV=1	[more]
A0A6J1ETX6	2.3e-48	58.70	stellacyanin-like OS=Cucurbita moschata OX=3662 GN=LOC111437535 PE=4 SV=1	[more]

BLAST of Moc10g08350 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G26330.1	1.1e-15	31.74	Cupredoxin superfamily protein	[more]
AT3G18590.1	8.1e-14	28.16	early nodulin-like protein 5	[more]
AT2G32300.1	2.0e-12	29.34	uclacyanin 1	[more]
AT3G20570.1	8.4e-11	26.87	early nodulin-like protein 9	[more]
AT5G53870.1	4.2e-10	29.73	early nodulin-like protein 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003245	Phytocyanin domain	PFAM	PF02298	Cu_bind_like	coord: 35..119 e-value: 1.6E-19 score: 69.7
IPR003245	Phytocyanin domain	PROSITE	PS51485	PHYTOCYANIN	coord: 25..127 score: 32.810883
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 25..138 e-value: 2.6E-30 score: 106.8
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 24..125
None	No IPR available	PANTHER	PTHR33021:SF264	OS05G0570900 PROTEIN	coord: 4..145
None	No IPR available	PROSITE	PS51257	PROKAR_LIPOPROTEIN	coord: 1..15 score: 5.0
None	No IPR available	CDD	cd13920	Stellacyanin	coord: 25..125 e-value: 4.00132E-46 score: 146.689
IPR039391	Phytocyanin	PANTHER	PTHR33021	BLUE COPPER PROTEIN	coord: 4..145

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc10g08350.1	Moc10g08350.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0022900	electron transport chain
cellular_component	GO:0046658	anchored component of plasma membrane
molecular_function	GO:0009055	electron transfer activity