Moc10g08310 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc10g08310
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Ulp1-like peptidase
Location	chr10: 6090069 .. 6090449 (-)
RNA-Seq Expression	Moc10g08310
Synteny	Moc10g08310

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATATTGGCGGCAACCACTGGGTCATGATCGGGATTGATCTTGTGGAGGGTGATTTAATCGTATGGGACTCACTGCAAGCGATCACCCCACTGGAGGATCTCGAGAAGGCACTCAAGCCAATGTGCACGATAATCCCGGCGATTCTTCATTGGAGCGGGATGCTCGCACTTCGGCCTAACCTACCCACGGTGTCATGGAGGGTCCGAAGATGTACTGTACCTCAGCAAGCCGGGTTCACAGATTGCGGCATATTTTGTATTAGATTTTTCGAGTACGATGTAACTGGGTCAAAGATGGGCACTTTGACTCAAAGTAACATTTCTTTATTTCGTCGTCAATATGCTGTACAAATGTGGGCTCGCAGACTCTTTTTTTAG

mRNA sequence

ATGAATATTGGCGGCAACCACTGGGTCATGATCGGGATTGATCTTGTGGAGGGTGATTTAATCGTATGGGACTCACTGCAAGCGATCACCCCACTGGAGGATCTCGAGAAGGCACTCAAGCCAATGTGCACGATAATCCCGGCGATTCTTCATTGGAGCGGGATGCTCGCACTTCGGCCTAACCTACCCACGGTGTCATGGAGGGTCCGAAGATGTACTGTACCTCAGCAAGCCGGGTTCACAGATTGCGGCATATTTTGTATTAGATTTTTCGAGTACGATGTAACTGGGTCAAAGATGGGCACTTTGACTCAAAGTAACATTTCTTTATTTCGTCGTCAATATGCTGTACAAATGTGGGCTCGCAGACTCTTTTTTTAG

Coding sequence (CDS)

ATGAATATTGGCGGCAACCACTGGGTCATGATCGGGATTGATCTTGTGGAGGGTGATTTAATCGTATGGGACTCACTGCAAGCGATCACCCCACTGGAGGATCTCGAGAAGGCACTCAAGCCAATGTGCACGATAATCCCGGCGATTCTTCATTGGAGCGGGATGCTCGCACTTCGGCCTAACCTACCCACGGTGTCATGGAGGGTCCGAAGATGTACTGTACCTCAGCAAGCCGGGTTCACAGATTGCGGCATATTTTGTATTAGATTTTTCGAGTACGATGTAACTGGGTCAAAGATGGGCACTTTGACTCAAAGTAACATTTCTTTATTTCGTCGTCAATATGCTGTACAAATGTGGGCTCGCAGACTCTTTTTTTAG

Protein sequence

MNIGGNHWVMIGIDLVEGDLIVWDSLQAITPLEDLEKALKPMCTIIPAILHWSGMLALRPNLPTVSWRVRRCTVPQQAGFTDCGIFCIRFFEYDVTGSKMGTLTQSNISLFRRQYAVQMWARRLFF

Homology

[Close]

BLAST of Moc10g08310 vs. NCBI nr
Match: XP_022153247.1 (uncharacterized protein LOC111020782 [Momordica charantia])

HSP 1 Score: 246.1 bits (627), Expect = 1.6e-61
Identity = 117/126 (92.86%), Postives = 118/126 (93.65%), Query Frame = 0


Query: 1   MNIGGNHWVMIGIDLVEGDLIVWDSLQAITPLEDLEKALKPMCTIIPAILHWSGMLALRP 60
           MNIGGNHWVMIGIDLVEGDL VWDSLQAITPLEDLEKALKPMCTIIP ILHWSGMLALRP
Sbjct: 35  MNIGGNHWVMIGIDLVEGDLTVWDSLQAITPLEDLEKALKPMCTIIPVILHWSGMLALRP 94

Query: 61  NLPTVSWRVRRCTVPQQAGFTDCGIFCIRFFEYDVTGSKMGTLTQSNISLFRRQYAVQMW 120
            L TV WRVRRCTVPQQAGFTDCGIFC+RFFEYDVTGSKM TL QSNISLFRRQYAVQMW
Sbjct: 95  ILHTVPWRVRRCTVPQQAGFTDCGIFCVRFFEYDVTGSKMDTLIQSNISLFRRQYAVQMW 154

Query: 121 ARRLFF 127
           ARR FF
Sbjct: 155 ARRPFF 160

[Close]

BLAST of Moc10g08310 vs. NCBI nr
Match: XP_022158807.1 (uncharacterized protein LOC111025273 [Momordica charantia])

HSP 1 Score: 244.6 bits (623), Expect = 4.6e-61
Identity = 115/126 (91.27%), Postives = 118/126 (93.65%), Query Frame = 0


Query: 1   MNIGGNHWVMIGIDLVEGDLIVWDSLQAITPLEDLEKALKPMCTIIPAILHWSGMLALRP 60
           MNIGGNHWVMIGIDLVEGDL VWDSLQAITPLEDLEKALKPMCTIIPAILHWSG+LALRP
Sbjct: 125 MNIGGNHWVMIGIDLVEGDLTVWDSLQAITPLEDLEKALKPMCTIIPAILHWSGILALRP 184

Query: 61  NLPTVSWRVRRCTVPQQAGFTDCGIFCIRFFEYDVTGSKMGTLTQSNISLFRRQYAVQMW 120
           NLP V WRVRRCTVPQQAGFTDC IFC+RFFEYDV GSK+ TL QSNISLFRRQYAVQMW
Sbjct: 185 NLPMVPWRVRRCTVPQQAGFTDCSIFCVRFFEYDVIGSKIDTLIQSNISLFRRQYAVQMW 244

Query: 121 ARRLFF 127
           ARR FF
Sbjct: 245 ARRPFF 250

[Close]

BLAST of Moc10g08310 vs. NCBI nr
Match: XP_022148137.1 (uncharacterized protein LOC111016890 [Momordica charantia])

HSP 1 Score: 182.6 bits (462), Expect = 2.2e-42
Identity = 80/116 (68.97%), Postives = 95/116 (81.90%), Query Frame = 0


Query: 1   MNIGGNHWVMIGIDLVEGDLIVWDSLQAITPLEDLEKALKPMCTIIPAILHWSGMLALRP 60
           MN+GGNHWVM+GIDLV+GD+ VWDSLQ  TPL++LEK LKPMCTI+P +LH  G+ ++RP
Sbjct: 45  MNVGGNHWVMLGIDLVQGDITVWDSLQTATPLDELEKELKPMCTILPTLLHHGGIFSVRP 104

Query: 61  NLPTVSWRVRRCTVPQQAGFTDCGIFCIRFFEYDVTGSKMGTLTQSNISLFRRQYA 117
           +LP V WRVRR  VPQQ+  TDCGIF +R+FEYD TGS M TLTQ NI  FRRQYA
Sbjct: 105 DLPVVPWRVRRVRVPQQSSVTDCGIFYVRYFEYDATGSNMDTLTQDNIVYFRRQYA 160

[Close]

BLAST of Moc10g08310 vs. NCBI nr
Match: XP_022156568.1 (uncharacterized protein LOC111023442 [Momordica charantia])

HSP 1 Score: 155.6 bits (392), Expect = 2.8e-34
Identity = 69/123 (56.10%), Postives = 94/123 (76.42%), Query Frame = 0


Query: 2   NIGGNHWVMIGIDLVEGDLIVWDSLQAITPLEDLEKALKPMCTIIPAILHWSGMLALRPN 61
           N+ GNHWVMI ID VEG+++VWDSL+AIT    LE+ LK M T+IP++LH S ++A+RPN
Sbjct: 26  NVNGNHWVMICIDFVEGEIVVWDSLRAITSYASLEEQLKVMHTVIPSLLHKSQVIAVRPN 85

Query: 62  LPTVSWRVRRCT-VPQQAGFTDCGIFCIRFFEYDVTGSKMGTLTQSNISLFRRQYAVQMW 121
           LP   WR+RR T  P+Q    DCGIFC+++FEYDVT + + TL Q+N+S FRRQ+A Q+W
Sbjct: 86  LPMTPWRIRRVTSTPEQFSSGDCGIFCVKYFEYDVTWTSLETLRQNNMSYFRRQFAFQLW 145

Query: 122 ARR 124
           + +
Sbjct: 146 SNK 148

[Close]

BLAST of Moc10g08310 vs. NCBI nr
Match: XP_022141784.1 (uncharacterized protein LOC111012067 [Momordica charantia])

HSP 1 Score: 151.4 bits (381), Expect = 5.3e-33
Identity = 67/123 (54.47%), Postives = 94/123 (76.42%), Query Frame = 0


Query: 2   NIGGNHWVMIGIDLVEGDLIVWDSLQAITPLEDLEKALKPMCTIIPAILHWSGMLALRPN 61
           N+ GN+WVMI ID VEG+++VWDSL+AIT    LE+ L+ M T+IP++LH S ++A+ PN
Sbjct: 26  NVNGNYWVMICIDFVEGEIVVWDSLRAITLDASLEEQLEVMRTVIPSLLHKSQVIAVHPN 85

Query: 62  LPTVSWRVRRCT-VPQQAGFTDCGIFCIRFFEYDVTGSKMGTLTQSNISLFRRQYAVQMW 121
           LP + WR+RR T  PQQ G  DC IFC+++FEYDVTG+ + TL Q+N+  FRRQ+A Q+W
Sbjct: 86  LPMIPWRIRRVTSAPQQFGSRDCDIFCVKYFEYDVTGTSLETLRQNNMPYFRRQFAFQLW 145

Query: 122 ARR 124
           + +
Sbjct: 146 SNK 148

[Close]

BLAST of Moc10g08310 vs. ExPASy TrEMBL
Match: A0A6J1DID7 (uncharacterized protein LOC111020782 OS=Momordica charantia OX=3673 GN=LOC111020782 PE=3 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 7.7e-62
Identity = 117/126 (92.86%), Postives = 118/126 (93.65%), Query Frame = 0


Query: 1   MNIGGNHWVMIGIDLVEGDLIVWDSLQAITPLEDLEKALKPMCTIIPAILHWSGMLALRP 60
           MNIGGNHWVMIGIDLVEGDL VWDSLQAITPLEDLEKALKPMCTIIP ILHWSGMLALRP
Sbjct: 35  MNIGGNHWVMIGIDLVEGDLTVWDSLQAITPLEDLEKALKPMCTIIPVILHWSGMLALRP 94

Query: 61  NLPTVSWRVRRCTVPQQAGFTDCGIFCIRFFEYDVTGSKMGTLTQSNISLFRRQYAVQMW 120
            L TV WRVRRCTVPQQAGFTDCGIFC+RFFEYDVTGSKM TL QSNISLFRRQYAVQMW
Sbjct: 95  ILHTVPWRVRRCTVPQQAGFTDCGIFCVRFFEYDVTGSKMDTLIQSNISLFRRQYAVQMW 154

Query: 121 ARRLFF 127
           ARR FF
Sbjct: 155 ARRPFF 160

[Close]

BLAST of Moc10g08310 vs. ExPASy TrEMBL
Match: A0A6J1DY60 (uncharacterized protein LOC111025273 OS=Momordica charantia OX=3673 GN=LOC111025273 PE=3 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 2.2e-61
Identity = 115/126 (91.27%), Postives = 118/126 (93.65%), Query Frame = 0


Query: 1   MNIGGNHWVMIGIDLVEGDLIVWDSLQAITPLEDLEKALKPMCTIIPAILHWSGMLALRP 60
           MNIGGNHWVMIGIDLVEGDL VWDSLQAITPLEDLEKALKPMCTIIPAILHWSG+LALRP
Sbjct: 125 MNIGGNHWVMIGIDLVEGDLTVWDSLQAITPLEDLEKALKPMCTIIPAILHWSGILALRP 184

Query: 61  NLPTVSWRVRRCTVPQQAGFTDCGIFCIRFFEYDVTGSKMGTLTQSNISLFRRQYAVQMW 120
           NLP V WRVRRCTVPQQAGFTDC IFC+RFFEYDV GSK+ TL QSNISLFRRQYAVQMW
Sbjct: 185 NLPMVPWRVRRCTVPQQAGFTDCSIFCVRFFEYDVIGSKIDTLIQSNISLFRRQYAVQMW 244

Query: 121 ARRLFF 127
           ARR FF
Sbjct: 245 ARRPFF 250

[Close]

BLAST of Moc10g08310 vs. ExPASy TrEMBL
Match: A0A6J1D492 (uncharacterized protein LOC111016890 OS=Momordica charantia OX=3673 GN=LOC111016890 PE=3 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 1.0e-42
Identity = 80/116 (68.97%), Postives = 95/116 (81.90%), Query Frame = 0


Query: 1   MNIGGNHWVMIGIDLVEGDLIVWDSLQAITPLEDLEKALKPMCTIIPAILHWSGMLALRP 60
           MN+GGNHWVM+GIDLV+GD+ VWDSLQ  TPL++LEK LKPMCTI+P +LH  G+ ++RP
Sbjct: 45  MNVGGNHWVMLGIDLVQGDITVWDSLQTATPLDELEKELKPMCTILPTLLHHGGIFSVRP 104

Query: 61  NLPTVSWRVRRCTVPQQAGFTDCGIFCIRFFEYDVTGSKMGTLTQSNISLFRRQYA 117
           +LP V WRVRR  VPQQ+  TDCGIF +R+FEYD TGS M TLTQ NI  FRRQYA
Sbjct: 105 DLPVVPWRVRRVRVPQQSSVTDCGIFYVRYFEYDATGSNMDTLTQDNIVYFRRQYA 160

[Close]

BLAST of Moc10g08310 vs. ExPASy TrEMBL
Match: A0A6J1DQZ3 (uncharacterized protein LOC111023442 OS=Momordica charantia OX=3673 GN=LOC111023442 PE=3 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 1.4e-34
Identity = 69/123 (56.10%), Postives = 94/123 (76.42%), Query Frame = 0


Query: 2   NIGGNHWVMIGIDLVEGDLIVWDSLQAITPLEDLEKALKPMCTIIPAILHWSGMLALRPN 61
           N+ GNHWVMI ID VEG+++VWDSL+AIT    LE+ LK M T+IP++LH S ++A+RPN
Sbjct: 26  NVNGNHWVMICIDFVEGEIVVWDSLRAITSYASLEEQLKVMHTVIPSLLHKSQVIAVRPN 85

Query: 62  LPTVSWRVRRCT-VPQQAGFTDCGIFCIRFFEYDVTGSKMGTLTQSNISLFRRQYAVQMW 121
           LP   WR+RR T  P+Q    DCGIFC+++FEYDVT + + TL Q+N+S FRRQ+A Q+W
Sbjct: 86  LPMTPWRIRRVTSTPEQFSSGDCGIFCVKYFEYDVTWTSLETLRQNNMSYFRRQFAFQLW 145

Query: 122 ARR 124
           + +
Sbjct: 146 SNK 148

[Close]

BLAST of Moc10g08310 vs. ExPASy TrEMBL
Match: A0A6J1CJT2 (uncharacterized protein LOC111012067 OS=Momordica charantia OX=3673 GN=LOC111012067 PE=3 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 2.6e-33
Identity = 67/123 (54.47%), Postives = 94/123 (76.42%), Query Frame = 0


Query: 2   NIGGNHWVMIGIDLVEGDLIVWDSLQAITPLEDLEKALKPMCTIIPAILHWSGMLALRPN 61
           N+ GN+WVMI ID VEG+++VWDSL+AIT    LE+ L+ M T+IP++LH S ++A+ PN
Sbjct: 26  NVNGNYWVMICIDFVEGEIVVWDSLRAITLDASLEEQLEVMRTVIPSLLHKSQVIAVHPN 85

Query: 62  LPTVSWRVRRCT-VPQQAGFTDCGIFCIRFFEYDVTGSKMGTLTQSNISLFRRQYAVQMW 121
           LP + WR+RR T  PQQ G  DC IFC+++FEYDVTG+ + TL Q+N+  FRRQ+A Q+W
Sbjct: 86  LPMIPWRIRRVTSAPQQFGSRDCDIFCVKYFEYDVTGTSLETLRQNNMPYFRRQFAFQLW 145

Query: 122 ARR 124
           + +
Sbjct: 146 SNK 148

[Close]

BLAST of Moc10g08310 vs. TAIR 10
Match: AT5G45570.1 (Ulp1 protease family protein )

HSP 1 Score: 56.2 bits (134), Expect = 2.2e-08
Identity = 37/121 (30.58%), Postives = 61/121 (50.41%), Query Frame = 0


Query: 3   IGGNHWVMIGIDLVEGDLIVWDSLQAITPLEDLEKALKPM--CTIIPAIL-HWSGMLALR 62
           + GNHWV + IDL    + V+DS+ ++T   D E A++ M   T+IPA+L  +      R
Sbjct: 777 VNGNHWVALDIDLTNKRVNVYDSIPSLT--TDTEMAIQCMFVMTMIPAMLSSFIPSKQRR 836

Query: 63  PNLPTVSWRVRRCTVPQQAGFTDCGIFCIRFFEYDVTGSKMGTLTQSNISLFRRQYAVQM 121
            +   + W+ R   +P+     DC I+ I++ E    G     L   N+   R + AV+M
Sbjct: 837 RSYSKLEWK-RITKIPENLDPGDCAIYSIKYIECLALGKSFDGLCDENMQSLRTKLAVEM 894

[Close]

BLAST of Moc10g08310 vs. TAIR 10
Match: AT4G08430.1 (Ulp1 protease family protein )

HSP 1 Score: 52.8 bits (125), Expect = 2.4e-07
Identity = 32/119 (26.89%), Postives = 58/119 (48.74%), Query Frame = 0


Query: 3   IGGNHWVMIGIDLVEGDLIVWDSLQAITPLEDLEKALKPMCTIIPAIL-HWSGMLALRPN 62
           + GNHWV + IDL +  + V+DS+ ++T   ++      + T+IPA+L  +      R +
Sbjct: 664 VNGNHWVALDIDLPKKRINVYDSIPSLTTDTEMVIQCMFVMTMIPAMLSSFIPSKQRRRS 723

Query: 63  LPTVSWRVRRCTVPQQAGFTDCGIFCIRFFEYDVTGSKMGTLTQSNISLFRRQYAVQMW 121
              + W+ R   +P+     DC I+ I++ E    G     L   N+     + AV+M+
Sbjct: 724 YSKLEWK-RITKIPENLDACDCAIYSIKYIECLALGKSFDGLCDENMQSLWTKLAVEMF 781

The following BLAST results are available for this feature:

BLAST of Moc10g08310 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022153247.1	1.6e-61	92.86	uncharacterized protein LOC111020782 [Momordica charantia]	[more]
XP_022158807.1	4.6e-61	91.27	uncharacterized protein LOC111025273 [Momordica charantia]	[more]
XP_022148137.1	2.2e-42	68.97	uncharacterized protein LOC111016890 [Momordica charantia]	[more]
XP_022156568.1	2.8e-34	56.10	uncharacterized protein LOC111023442 [Momordica charantia]	[more]
XP_022141784.1	5.3e-33	54.47	uncharacterized protein LOC111012067 [Momordica charantia]	[more]

BLAST of Moc10g08310 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Moc10g08310 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DID7	7.7e-62	92.86	uncharacterized protein LOC111020782 OS=Momordica charantia OX=3673 GN=LOC111020...	[more]
A0A6J1DY60	2.2e-61	91.27	uncharacterized protein LOC111025273 OS=Momordica charantia OX=3673 GN=LOC111025...	[more]
A0A6J1D492	1.0e-42	68.97	uncharacterized protein LOC111016890 OS=Momordica charantia OX=3673 GN=LOC111016...	[more]
A0A6J1DQZ3	1.4e-34	56.10	uncharacterized protein LOC111023442 OS=Momordica charantia OX=3673 GN=LOC111023...	[more]
A0A6J1CJT2	2.6e-33	54.47	uncharacterized protein LOC111012067 OS=Momordica charantia OX=3673 GN=LOC111012...	[more]

BLAST of Moc10g08310 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G45570.1	2.2e-08	30.58	Ulp1 protease family protein	[more]
AT4G08430.1	2.4e-07	26.89	Ulp1 protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003653	Ulp1 protease family, C-terminal catalytic domain	PFAM	PF02902	Peptidase_C48	coord: 1..119 e-value: 4.1E-16 score: 59.5
IPR003653	Ulp1 protease family, C-terminal catalytic domain	PROSITE	PS50600	ULP_PROTEASE	coord: 1..94 score: 11.311567
None	No IPR available	GENE3D	3.40.395.10	Adenoviral Proteinase; Chain A	coord: 1..125 e-value: 1.6E-17 score: 65.7
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	54001	Cysteine proteinases	coord: 1..121

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc10g08310.1	Moc10g08310.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0008234	cysteine-type peptidase activity