Moc10g05480 (gene) Bitter gourd (OHB3-1) v2

BLAST of Moc10g05480 vs. NCBI nr
Match: XP_022136577.1 (MACPF domain-containing protein At4g24290 [Momordica charantia] >XP_022136578.1 MACPF domain-containing protein At4g24290 [Momordica charantia])

HSP 1 Score: 1177.9 bits (3046), Expect = 0.0e+00
Identity = 580/580 (100.00%), Postives = 580/580 (100.00%), Query Frame = 0

Query: 1   MALNFPASKAAEIAIASIGRGYDITADLRLKYCKWDLHRSRLIEIDEDVGHEIVLPGGIS 60
           MALNFPASKAAEIAIASIGRGYDITADLRLKYCKWDLHRSRLIEIDEDVGHEIVLPGGIS
Sbjct: 1   MALNFPASKAAEIAIASIGRGYDITADLRLKYCKWDLHRSRLIEIDEDVGHEIVLPGGIS 60

Query: 61  IPNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           IPNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD
Sbjct: 61  IPNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120

Query: 121 ASNTKNLAFDGVFITLYTVALEKSQMVLCDLVRKAVPSSWDPAALARFIETFGTHIIVGL 180
           ASNTKNLAFDGVFITLYTVALEKSQMVLCDLVRKAVPSSWDPAALARFIETFGTHIIVGL
Sbjct: 121 ASNTKNLAFDGVFITLYTVALEKSQMVLCDLVRKAVPSSWDPAALARFIETFGTHIIVGL 180

Query: 181 KMGGKDVIYMKQQHSSTLQPSEVQKRLKEMADKRFSETIGPYGMDSERIYNNDRDIATIF 240
           KMGGKDVIYMKQQHSSTLQPSEVQKRLKEMADKRFSETIGPYGMDSERIYNNDRDIATIF
Sbjct: 181 KMGGKDVIYMKQQHSSTLQPSEVQKRLKEMADKRFSETIGPYGMDSERIYNNDRDIATIF 240

Query: 241 KRRGGIESINLPHHEWLQTVQLGPDVISMSFIPITSLLNGVPGSGFLSHAINLYLRYKPP 300
           KRRGGIESINLPHHEWLQTVQLGPDVISMSFIPITSLLNGVPGSGFLSHAINLYLRYKPP
Sbjct: 241 KRRGGIESINLPHHEWLQTVQLGPDVISMSFIPITSLLNGVPGSGFLSHAINLYLRYKPP 300

Query: 301 IEELHQFLEFQLPRQWAPIFSELPLGPQRKHQNLASLQFSLMGSKLYVNTTPVDVGRRPV 360
           IEELHQFLEFQLPRQWAPIFSELPLGPQRKHQNLASLQFSLMGSKLYVNTTPVDVGRRPV
Sbjct: 301 IEELHQFLEFQLPRQWAPIFSELPLGPQRKHQNLASLQFSLMGSKLYVNTTPVDVGRRPV 360

Query: 361 TGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPNGNFHPESYDRRYLEKVQSKYFSHV 420
           TGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPNGNFHPESYDRRYLEKVQSKYFSHV
Sbjct: 361 TGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPNGNFHPESYDRRYLEKVQSKYFSHV 420

Query: 421 CTAPVEADEDLSIVTGAQLQVVNHGMRNILFLRLRFSSVLGATVVKQAEWEGSPGLAAKS 480
           CTAPVEADEDLSIVTGAQLQVVNHGMRNILFLRLRFSSVLGATVVKQAEWEGSPGLAAKS
Sbjct: 421 CTAPVEADEDLSIVTGAQLQVVNHGMRNILFLRLRFSSVLGATVVKQAEWEGSPGLAAKS 480

Query: 481 GLISTLISHHFSTVQKPPPKPADVNINSAIYPGGPPVPVQAPKLLKFVDTTEMKRGPQET 540
           GLISTLISHHFSTVQKPPPKPADVNINSAIYPGGPPVPVQAPKLLKFVDTTEMKRGPQET
Sbjct: 481 GLISTLISHHFSTVQKPPPKPADVNINSAIYPGGPPVPVQAPKLLKFVDTTEMKRGPQET 540

Query: 541 PGYWVVSGARLMVEKGRISLRVKFSLLTIILPDEEEINDN 581
           PGYWVVSGARLMVEKGRISLRVKFSLLTIILPDEEEINDN
Sbjct: 541 PGYWVVSGARLMVEKGRISLRVKFSLLTIILPDEEEINDN 580

BLAST of Moc10g05480 vs. NCBI nr
Match: XP_023552323.1 (MACPF domain-containing protein At4g24290-like [Cucurbita pepo subsp. pepo] >XP_023552324.1 MACPF domain-containing protein At4g24290-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1090.5 bits (2819), Expect = 0.0e+00
Identity = 537/580 (92.59%), Postives = 553/580 (95.34%), Query Frame = 0

Query: 1   MALNFPASKAAEIAIASIGRGYDITADLRLKYCKWDLHRSRLIEIDEDVGHEIVLPGGIS 60
           MAL    SKAAEIAI SIGRGYDIT DLRLKYCK + H SRLIEIDE VGHEIVLPGGI 
Sbjct: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNFHGSRLIEIDEGVGHEIVLPGGIL 60

Query: 61  IPNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           I NVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGH NTMFEFSGCWQKD
Sbjct: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHLNTMFEFSGCWQKD 120

Query: 121 ASNTKNLAFDGVFITLYTVALEKSQMVLCDLVRKAVPSSWDPAALARFIETFGTHIIVGL 180
           A+NTKNLAFDGVFITLYTVALEKSQMVLCD VRKAVPS+WDPAALARFIETFGTHIIVGL
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSTWDPAALARFIETFGTHIIVGL 180

Query: 181 KMGGKDVIYMKQQHSSTLQPSEVQKRLKEMADKRFSETIGPYGMDSERIYNNDRDIATIF 240
           KMGGKDVIYMKQQHSSTLQPSEVQKRLKEMADKRFSETIG YGM SE+IYNND+DI TI+
Sbjct: 181 KMGGKDVIYMKQQHSSTLQPSEVQKRLKEMADKRFSETIGQYGMGSEQIYNNDKDILTIY 240

Query: 241 KRRGGIESINLPHHEWLQTVQLGPDVISMSFIPITSLLNGVPGSGFLSHAINLYLRYKPP 300
           KRRGG + +N PHHEWLQTVQL PDVISMSFIPITSLLNGVPGSGFLSHAINLYLRYKPP
Sbjct: 241 KRRGGSDRMNYPHHEWLQTVQLEPDVISMSFIPITSLLNGVPGSGFLSHAINLYLRYKPP 300

Query: 301 IEELHQFLEFQLPRQWAPIFSELPLGPQRKHQNLASLQFSLMGSKLYVNTTPVDVGRRPV 360
           IEELHQFLEFQLPRQWAPIFSELPLGPQRK  NLASLQFSLMGSKLYVNTTPVDVGRRPV
Sbjct: 301 IEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLASLQFSLMGSKLYVNTTPVDVGRRPV 360

Query: 361 TGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPNGNFHPESYDRRYLEKVQSKYFSHV 420
           TGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPNGNF  ESYDRRYLEKVQSKYFSHV
Sbjct: 361 TGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPNGNFCQESYDRRYLEKVQSKYFSHV 420

Query: 421 CTAPVEADEDLSIVTGAQLQVVNHGMRNILFLRLRFSSVLGATVVKQAEWEGSPGLAAKS 480
           CTAP+EADEDLSIVTGAQLQVVNHGM+N+LFLRLRFSSVLGATVV+Q EWEGSPGLAAKS
Sbjct: 421 CTAPIEADEDLSIVTGAQLQVVNHGMKNVLFLRLRFSSVLGATVVRQPEWEGSPGLAAKS 480

Query: 481 GLISTLISHHFSTVQKPPPKPADVNINSAIYPGGPPVPVQAPKLLKFVDTTEMKRGPQET 540
           GLISTLISHHFSTVQKPPPKPADV INSA+YPGGPPVPVQAPKLLKFVDTTEMKRGPQ+T
Sbjct: 481 GLISTLISHHFSTVQKPPPKPADVIINSAVYPGGPPVPVQAPKLLKFVDTTEMKRGPQDT 540

Query: 541 PGYWVVSGARLMVEKGRISLRVKFSLLTIILPDEEEINDN 581
           PGYWVVSGARLMVEKGRISLRV+FSLLT+ILPDEEEINDN
Sbjct: 541 PGYWVVSGARLMVEKGRISLRVRFSLLTVILPDEEEINDN 580

BLAST of Moc10g05480 vs. NCBI nr
Match: XP_022984562.1 (MACPF domain-containing protein At4g24290-like [Cucurbita maxima] >XP_022984563.1 MACPF domain-containing protein At4g24290-like [Cucurbita maxima])

HSP 1 Score: 1085.9 bits (2807), Expect = 0.0e+00
Identity = 535/580 (92.24%), Postives = 550/580 (94.83%), Query Frame = 0

Query: 1   MALNFPASKAAEIAIASIGRGYDITADLRLKYCKWDLHRSRLIEIDEDVGHEIVLPGGIS 60
           MAL    SKAAEIAI SIGRGYDIT DLRLKYCK + H SRLIEIDE VGHEIVLPGGI 
Sbjct: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNFHGSRLIEIDEGVGHEIVLPGGIL 60

Query: 61  IPNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           I NVPKSIKCDKGERTRFRSDVLSFQQMSE FNQEMTLTGKIPSGH NTMFEFSGCWQKD
Sbjct: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEHFNQEMTLTGKIPSGHLNTMFEFSGCWQKD 120

Query: 121 ASNTKNLAFDGVFITLYTVALEKSQMVLCDLVRKAVPSSWDPAALARFIETFGTHIIVGL 180
           A+NTKNLAFDGVFITLYTVALEKSQMVLCD VRK VPS+WDPAALARFIETFGTHIIVGL
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKEVPSTWDPAALARFIETFGTHIIVGL 180

Query: 181 KMGGKDVIYMKQQHSSTLQPSEVQKRLKEMADKRFSETIGPYGMDSERIYNNDRDIATIF 240
           KMGGKDVIYMKQQHSSTLQP+EVQKRLKEMADKRFSETIG YGM SE+IYNND+DI  I+
Sbjct: 181 KMGGKDVIYMKQQHSSTLQPTEVQKRLKEMADKRFSETIGQYGMGSEQIYNNDKDILAIY 240

Query: 241 KRRGGIESINLPHHEWLQTVQLGPDVISMSFIPITSLLNGVPGSGFLSHAINLYLRYKPP 300
           KRRGG + IN PHHEWLQTVQL PDVISMSFIPITSLLNGVPGSGFLSHAINLYLRYKPP
Sbjct: 241 KRRGGSDRINYPHHEWLQTVQLEPDVISMSFIPITSLLNGVPGSGFLSHAINLYLRYKPP 300

Query: 301 IEELHQFLEFQLPRQWAPIFSELPLGPQRKHQNLASLQFSLMGSKLYVNTTPVDVGRRPV 360
           IEELHQFLEFQLPRQWAPIFSELPLGPQRK  NLASLQFSLMGSKLYVNTTPVDVGRRPV
Sbjct: 301 IEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLASLQFSLMGSKLYVNTTPVDVGRRPV 360

Query: 361 TGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPNGNFHPESYDRRYLEKVQSKYFSHV 420
           TGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPNGNF  ESYDRRYLEKVQSKYFSHV
Sbjct: 361 TGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPNGNFCQESYDRRYLEKVQSKYFSHV 420

Query: 421 CTAPVEADEDLSIVTGAQLQVVNHGMRNILFLRLRFSSVLGATVVKQAEWEGSPGLAAKS 480
           CTAP+EADEDLSIVTGAQLQVVNHGM+N+LFLRLRFSSVLGATVV+Q EWEGSPGLAAKS
Sbjct: 421 CTAPIEADEDLSIVTGAQLQVVNHGMKNVLFLRLRFSSVLGATVVRQPEWEGSPGLAAKS 480

Query: 481 GLISTLISHHFSTVQKPPPKPADVNINSAIYPGGPPVPVQAPKLLKFVDTTEMKRGPQET 540
           GLISTLISHHFSTVQKPPPKPADV INSA+YPGGPPVPVQAPKLLKFVDTTEMKRGPQ+T
Sbjct: 481 GLISTLISHHFSTVQKPPPKPADVIINSAVYPGGPPVPVQAPKLLKFVDTTEMKRGPQDT 540

Query: 541 PGYWVVSGARLMVEKGRISLRVKFSLLTIILPDEEEINDN 581
           PGYWVVSGARLMVEKGRISLRVKFSLLT+ILPDEEEINDN
Sbjct: 541 PGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEEEINDN 580

BLAST of Moc10g05480 vs. NCBI nr
Match: XP_022929230.1 (MACPF domain-containing protein At4g24290-like [Cucurbita moschata] >XP_022929231.1 MACPF domain-containing protein At4g24290-like [Cucurbita moschata] >KAG7015456.1 MACPF domain-containing protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1085.5 bits (2806), Expect = 0.0e+00
Identity = 535/580 (92.24%), Postives = 551/580 (95.00%), Query Frame = 0

Query: 1   MALNFPASKAAEIAIASIGRGYDITADLRLKYCKWDLHRSRLIEIDEDVGHEIVLPGGIS 60
           MAL    SKAAEIAI SIGRGYDIT DLRLKYCK + H SRLIEIDE VGHEIVLPGGI 
Sbjct: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNFHGSRLIEIDEGVGHEIVLPGGIL 60

Query: 61  IPNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           I NVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGH NTMFEFSGCWQKD
Sbjct: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHLNTMFEFSGCWQKD 120

Query: 121 ASNTKNLAFDGVFITLYTVALEKSQMVLCDLVRKAVPSSWDPAALARFIETFGTHIIVGL 180
           A+NTKNLAFDGVFITLYTVALEKSQMVLCD VRKAVPS+WDPAALARFIETFGTHIIVGL
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSTWDPAALARFIETFGTHIIVGL 180

Query: 181 KMGGKDVIYMKQQHSSTLQPSEVQKRLKEMADKRFSETIGPYGMDSERIYNNDRDIATIF 240
           KMGGKDVIYMKQQH STLQPSEVQKRLKEMADKRFSETIG YGM SE+IYN+D+DI  I+
Sbjct: 181 KMGGKDVIYMKQQHYSTLQPSEVQKRLKEMADKRFSETIGQYGMGSEQIYNSDKDILAIY 240

Query: 241 KRRGGIESINLPHHEWLQTVQLGPDVISMSFIPITSLLNGVPGSGFLSHAINLYLRYKPP 300
           KRRGG + IN PHHEWLQTVQL PDVISMSFIPITSLLNGVPGSGFLSHAINLYLRYKPP
Sbjct: 241 KRRGGSDRINYPHHEWLQTVQLEPDVISMSFIPITSLLNGVPGSGFLSHAINLYLRYKPP 300

Query: 301 IEELHQFLEFQLPRQWAPIFSELPLGPQRKHQNLASLQFSLMGSKLYVNTTPVDVGRRPV 360
           IEELHQFLEFQLPRQWAPIFSELPLGPQRK  NLASLQFSLMGSKLYVNTTPVDVGRRPV
Sbjct: 301 IEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLASLQFSLMGSKLYVNTTPVDVGRRPV 360

Query: 361 TGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPNGNFHPESYDRRYLEKVQSKYFSHV 420
           TGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPNGNF  ESYDRRYLEKVQSKYFSHV
Sbjct: 361 TGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPNGNFCQESYDRRYLEKVQSKYFSHV 420

Query: 421 CTAPVEADEDLSIVTGAQLQVVNHGMRNILFLRLRFSSVLGATVVKQAEWEGSPGLAAKS 480
           CTAP+EADEDLSIVTGAQLQVVNHGM+N+LFLRLRFSSVLGATVV+Q EWEGSPGLAAKS
Sbjct: 421 CTAPIEADEDLSIVTGAQLQVVNHGMKNVLFLRLRFSSVLGATVVRQPEWEGSPGLAAKS 480

Query: 481 GLISTLISHHFSTVQKPPPKPADVNINSAIYPGGPPVPVQAPKLLKFVDTTEMKRGPQET 540
           GLISTLISHHFSTVQKPPPKPADV INSA+YPGGPPVPVQAPKLLKFVDTTEMKRGPQ+T
Sbjct: 481 GLISTLISHHFSTVQKPPPKPADVIINSAVYPGGPPVPVQAPKLLKFVDTTEMKRGPQDT 540

Query: 541 PGYWVVSGARLMVEKGRISLRVKFSLLTIILPDEEEINDN 581
           PGYWVVSGARLMVEKGRISLRV+FSLLT+ILPDEEEINDN
Sbjct: 541 PGYWVVSGARLMVEKGRISLRVRFSLLTVILPDEEEINDN 580

BLAST of Moc10g05480 vs. NCBI nr
Match: XP_004133715.1 (MACPF domain-containing protein At4g24290 [Cucumis sativus] >XP_011650569.1 MACPF domain-containing protein At4g24290 [Cucumis sativus] >KAE8650184.1 hypothetical protein Csa_010735 [Cucumis sativus])

HSP 1 Score: 1075.5 bits (2780), Expect = 0.0e+00
Identity = 541/604 (89.57%), Postives = 552/604 (91.39%), Query Frame = 0

Query: 1   MALNFPASKAAEIAIASIGRGYDITADLRLKYCKWDLHRSRLIEIDEDVGHEIVLPGGIS 60
           MAL    SKAAEIAI SIGRGYDIT DLRLKYCK +LH SRLIEIDE VGHEIVLPGGI 
Sbjct: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIL 60

Query: 61  IPNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           I NVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD
Sbjct: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120

Query: 121 ASNTKNLAFDGVFITLYTVALEKSQMVLCDLVRKAVPSSWDPAALARFIETFGTHIIVGL 180
           A+NTKNLAFDGVFITLYTVALEKSQMVLCD VRKAVPSSWDPAALARFIETFGTHIIVGL
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180

Query: 181 KMGGKDVIYMKQQHSSTLQ---PSEVQKRLKEMADKRFSETIGPYGMDSERIYNND---- 240
           KMGGKDVIYMKQQHSSTLQ   PSEVQK LKEMADKRFSETI  YGM SER+YNND    
Sbjct: 181 KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKIEI 240

Query: 241 -----------------RDIATIFKRRGGIESINLPHHEWLQTVQLGPDVISMSFIPITS 300
                            +DI  I KRRGG + I+LPHHEWLQTVQL PDVISMSFIPITS
Sbjct: 241 REQHLRFGDASSAISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITS 300

Query: 301 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKHQNLAS 360
           LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRK  NLAS
Sbjct: 301 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS 360

Query: 361 LQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPN 420
           LQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPN
Sbjct: 361 LQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPN 420

Query: 421 GNFHPESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNILFLRLRF 480
           GNF  +SYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVN GMRN+LFLRLRF
Sbjct: 421 GNFRQDSYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNQGMRNVLFLRLRF 480

Query: 481 SSVLGATVVKQAEWEGSPGLAAKSGLISTLISHHFSTVQKPPPKPADVNINSAIYPGGPP 540
           SSVLGATVV+QAEWEGSPGLAAKSGLISTLISHHFSTV KPPPKPADVNINSA+YPGGPP
Sbjct: 481 SSVLGATVVRQAEWEGSPGLAAKSGLISTLISHHFSTVMKPPPKPADVNINSAVYPGGPP 540

Query: 541 VPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTIILPDEEE 581
           VPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLT+ILPDEEE
Sbjct: 541 VPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEEE 600

BLAST of Moc10g05480 vs. ExPASy Swiss-Prot
Match: Q9STW5 (MACPF domain-containing protein At4g24290 OS=Arabidopsis thaliana OX=3702 GN=At4g24290 PE=2 SV=1)

HSP 1 Score: 862.1 bits (2226), Expect = 3.7e-249
Identity = 429/598 (71.74%), Postives = 491/598 (82.11%), Query Frame = 0

Query: 1   MALNFPASKAAEIAIASIGRGYDITADLRLKYCKWDLHRSRLIEIDE-DVGHEIVLPGGI 60
           MAL  PASKAAE+AI SIG GYD+  DLRLKYCK     SRL++I E D   EIVLPGGI
Sbjct: 1   MALRLPASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGI 60

Query: 61  SIPNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQK 120
           SIPNV KSIKCDKGER RFRSD+L FQQM+EQFNQE++L GKIPSG FN MFEFS CWQK
Sbjct: 61  SIPNVSKSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQK 120

Query: 121 DASNTKNLAFDGVFITLYTVALEKSQMVLCDLVRKAVPSSWDPAALARFIETFGTHIIVG 180
           DA+ TKNLAFDGVFI+LY+VAL+KSQ++L + V++AVPS+WDPAALARFI+ +GTHIIV 
Sbjct: 121 DAAYTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVS 180

Query: 181 LKMGGKDVIYMKQQHSSTLQPSEVQKRLKEMADKRFSETIGPYGMDSERI---------- 240
           +KMGGKDVIY KQQHSS LQP ++QKRLKE+ADKRF E    +   SER+          
Sbjct: 181 VKMGGKDVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKE 240

Query: 241 -------------YNNDRDIATIFKRRGGIESINLPHHEWLQTVQLGPDVISMSFIPITS 300
                        Y N  D   + KRRGG ++ NL H+EWLQTVQ+ PDVISMSFIPITS
Sbjct: 241 QRLRFADTSSLGSYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITS 300

Query: 301 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKHQNLAS 360
           LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAP+FSELPLGPQRK Q+ AS
Sbjct: 301 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQSCAS 360

Query: 361 LQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPN 420
           LQFS  G KLYVNTTPVDVG+RP+TG+RLYLEG+RSN L+IHLQHLSSLPKI+QL +D N
Sbjct: 361 LQFSFFGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLEDDLN 420

Query: 421 GNFHPESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNILFLRLRF 480
            +   ES+DRRY EKV  K +SHVCT PVE+D+DLS+VTGAQL V +HG +N+LFLRL F
Sbjct: 421 RSIRQESHDRRYYEKVNWKNYSHVCTEPVESDDDLSVVTGAQLHVESHGFKNVLFLRLCF 480

Query: 481 SSVLGATVVKQAEWEGSPGLAAKSGLISTLISHHFSTVQKPPPKPADVNINSAIYPGGPP 540
           S V+GAT+VK +EW+ + G A KSGLISTLISHHF+  QKPPP+PADVNINSAIYPGGPP
Sbjct: 481 SRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPPRPADVNINSAIYPGGPP 540

Query: 541 VPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTIILPDE 575
           VP QAPKLLKFVDT+EM RGPQE+PGYWVVSGARL+VEKG+ISL+VK+SL T IL DE
Sbjct: 541 VPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKVKYSLFTPILGDE 598

BLAST of Moc10g05480 vs. ExPASy Swiss-Prot
Match: Q9SGN6 (MACPF domain-containing protein NSL1 OS=Arabidopsis thaliana OX=3702 GN=NSL1 PE=2 SV=1)

HSP 1 Score: 591.7 bits (1524), Expect = 9.3e-168
Identity = 314/603 (52.07%), Postives = 405/603 (67.16%), Query Frame = 0

Query: 7   ASKAAEIAIASIGRGYDITADLRLKYCKWDLHRSRLIEIDEDVGHEIVLPGGISIPNVPK 66
           A  AAE A++ IG GYD+ +D+R   CK     SRL+EID     +++ PGGI + NV  
Sbjct: 11  AHSAAEKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIVVNNVSS 70

Query: 67  SIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKDASNTKN 126
           SIKCDKGERTR RSD+LSF QMSE+FNQ+M L+GKIPSG FN MF FS CW KDAS+ K 
Sbjct: 71  SIKCDKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKDASSVKT 130

Query: 127 LAFDGVFITLYTVALEKSQMVLCDLVRKAVPSSWDPAALARFIETFGTHIIVGLKMGGKD 186
           LA+DG FI+LY+V + + Q+ L D V++ VPSSWD AALA FIE +GTH++VG+ MGGKD
Sbjct: 131 LAYDGWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGGKD 190

Query: 187 VIYMKQQHSSTLQPSEVQKRLKEMADKRFS----ETIGPYGMDSER-------------- 246
           VI++KQ   S  +P E+QK LK   D+RF     E+  P  + S +              
Sbjct: 191 VIHVKQMRKSNHEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPKEENLLQWGLQPF 250

Query: 247 ---------IYNNDRDIATIFKRRGGIESINLPHHEWLQTVQLGPDVISMSFIPITSLLN 306
                    ++  + +I  +  RRGG++ +   H  WL TV   P+VISM F+PITSLL+
Sbjct: 251 GTSVSSAVVMHTKNEEIMRVCIRRGGVD-LGQSHERWLSTVSQAPNVISMCFVPITSLLS 310

Query: 307 GVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKHQNLASLQF 366
           G+PG+GFLSHA+NLYLRYKPPIEELHQFLEFQLPRQWAP++ +LPLG +R  Q+  SLQF
Sbjct: 311 GLPGTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSPSLQF 370

Query: 367 SLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPNGNF 426
           SLMG KLYVNT+ VD G RPVTGLR +LEGK+ N L+IHLQHLS+ P    L+ D     
Sbjct: 371 SLMGPKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSHDDTYEP 430

Query: 427 HPESYDRRYLEKVQSKYFSHVCTAPVE-----ADEDLSIVTGAQLQVVNHGMRNILFLRL 486
             E  ++ Y   V+   FSHVCT PV+     +D+  SIVT A L+V   GMR +LFLRL
Sbjct: 431 IEEPVEKGYYVPVKWGIFSHVCTYPVQYNGARSDDTASIVTKAWLEVKGMGMRKVLFLRL 490

Query: 487 RFSSVLGATVVKQAEWEGSPGLAAKSGLISTLISHHFSTVQKPPP---KP-ADVNINSAI 546
            F S+  + V +++ W+     + KSG+ S +IS   ST   P P   KP + ++INSA+
Sbjct: 491 GF-SLDASAVTRKSCWDNLSTNSRKSGVFS-MISTRLSTGLSPNPATTKPQSKIDINSAV 550

Query: 547 YPGGPPVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTII 574
           YP GP  PV+ PKLL  VDT E+ RGP+E PGYWVV+GA+L VE G+IS++ K+SLLT+I
Sbjct: 551 YPRGPSPPVK-PKLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIKAKYSLLTVI 609

BLAST of Moc10g05480 vs. ExPASy Swiss-Prot
Match: Q9C7N2 (MACPF domain-containing protein CAD1 OS=Arabidopsis thaliana OX=3702 GN=CAD1 PE=2 SV=1)

HSP 1 Score: 464.5 bits (1194), Expect = 1.7e-129
Identity = 266/582 (45.70%), Postives = 354/582 (60.82%), Query Frame = 0

Query: 14  AIASIGRGYDITADLRLKYCKWDLHRSRLIEIDEDVGHEIVLPGGISIPNVPKSIKCDKG 73
           AI ++GRG+D+T+D+RL YCK     SRL+ I+E    ++ L  G  +PNVP  I C +G
Sbjct: 24  AIQALGRGFDVTSDVRLLYCK-GAPGSRLVRIEEGQNRDLELSHGFLLPNVPADIDCSRG 83

Query: 74  ERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKDASNTKNLAFDGVF 133
                R  V SF +M+E+FN    + G IP G FN MF ++G WQ DA++TK+LA  G F
Sbjct: 84  NSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAASTKSLALVGYF 143

Query: 134 ITLYTVALEKSQMVLCDLVRKAVPSSWDPAALARFIETFGTHIIVGLKMGGKDVIYMKQQ 193
           I LY V L K  +VL + +R+AVPSSWDPA+LA FIE +GTHI+  + +GG+DV+Y++Q 
Sbjct: 144 IPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYIRQH 203

Query: 194 HSSTLQPSEVQKRLKEMADKRFSETIGPYGMDSERIYN----NDRDIATIFKRRGGIESI 253
            SS L  SE++  + +M   RF E       +S+ I       D+DI  IF+RRGG + +
Sbjct: 204 QSSPLPVSEIENYVNDMIKHRFHEA------ESQSITGPLKYKDKDITVIFRRRGG-DDL 263

Query: 254 NLPHHEWLQTVQLGPDVISMSFIPITSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLE 313
              H  W +TV   PD+I+M+F PI SLL GVPG   L+ AI LYL YKPPIE+L  FL+
Sbjct: 264 EQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHLTRAIELYLEYKPPIEDLQYFLD 323

Query: 314 FQLPRQWAPIFSELPLGPQRKHQNLASLQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEG 373
           +Q+ R WAP  S L    QRK    +SLQFSLMG KL+++   V VGR+PVTGLRL LEG
Sbjct: 324 YQIARAWAPEQSNL----QRKEPVCSSLQFSLMGPKLFISADQVTVGRKPVTGLRLSLEG 383

Query: 374 KRSNCLSIHLQHLSSLPKIFQLTEDPNGNFH----------PESYDRRYLEKVQSKYFSH 433
            + N LSIHLQHL SLPKI Q    P+ + H          PE  D R+ E ++ K FSH
Sbjct: 384 SKQNRLSIHLQHLVSLPKILQ----PHWDSHVPIGAPKWQGPEEQDSRWFEPIKWKNFSH 443

Query: 434 VCTAPVEADE----DLS---IVTGAQLQVVNHGMRNILFLRLRFSSVLGATVVKQAEWEG 493
           V T+P+E  E    DLS   IVTGAQL V + G +N+L L+L FS V G T ++++ W+ 
Sbjct: 444 VSTSPIEHTETHIGDLSGVHIVTGAQLGVWDFGSKNVLHLKLLFSKVPGCT-IRRSVWDH 503

Query: 494 SPGLAAKSGLISTLISHHFSTVQKPPPKPADVNINSAIYPGGP-------PVPVQAPKLL 553
           +P   A SG +                            PGGP        V  Q+ KL 
Sbjct: 504 TP--VASSGRLE---------------------------PGGPSTSSSTEEVSGQSGKLA 559

Query: 554 KFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLL 568
           K VD++EM +GPQ+ PG+W+V+GA+L VEKG+I LRVK+SLL
Sbjct: 564 KIVDSSEMLKGPQDLPGHWLVTGAKLGVEKGKIVLRVKYSLL 559

BLAST of Moc10g05480 vs. ExPASy Swiss-Prot
Match: Q8L612 (MACPF domain-containing protein At1g14780 OS=Arabidopsis thaliana OX=3702 GN=At1g14780 PE=2 SV=1)

HSP 1 Score: 462.2 bits (1188), Expect = 8.5e-129
Identity = 262/617 (42.46%), Postives = 363/617 (58.83%), Query Frame = 0

Query: 12  EIAIASIGRGYDITADLRLKYCK---WDLHRSRLIEIDEDVGHEIVLPGGISIPNVPKSI 71
           E A+ S+G+G+D+TAD RLKYCK         RL+ +D+    E+ +PG     NV   I
Sbjct: 10  ETAVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADI 69

Query: 72  KCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEF-SGCWQKDASNTKNL 131
            CDKGERTRFRSD+L F +MSE FNQ  ++TGKIPSG+FN  F F SG W  DA+N K+L
Sbjct: 70  NCDKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSL 129

Query: 132 AFDGVFITLYTVALEK-SQMVLCDLVRKAVPSSWDPAALARFIETFGTHIIVGLKMGGKD 191
             D   +TL+ + +   +++ L D VR AVPSSWDP  LARFIE +GTH+I G+ +GG+D
Sbjct: 130 GLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQD 189

Query: 192 VIYMKQQHSSTLQPSEVQKRLKEMADK------------------------RFSETIGPY 251
           V+ ++Q  SS L    ++  L ++ D+                        +F E    +
Sbjct: 190 VVVVRQDKSSDLDNDLLRHHLYDLGDQLFTGSCLLSTRRLNKAYHHSHSQPKFPEAFNVF 249

Query: 252 GMDSERIYNN-----DRDIATIFKRRGGIESINLPHHEWLQTVQLGPDVISMSFIPITSL 311
                  +NN        I  I  +RGG +     H EWL TV   PD I+ +FIPITSL
Sbjct: 250 DDKQTVAFNNFSINSQNGITVICAKRGG-DGRAKSHSEWLITVPDKPDAINFNFIPITSL 309

Query: 312 LNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKHQNLAS- 371
           L  VPGSG LSHA++LYLRYKPP+ +L  FL+F  PR WAP+ ++LP G      N+AS 
Sbjct: 310 LKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAA---PNMASA 369

Query: 372 ---LQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIF--QL 431
              L  + MG KLYVNTTPV   + PVTG+R +LEGK+ N L+IHLQHL +       ++
Sbjct: 370 YPALHINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGEKI 429

Query: 432 TEDPNGNFHPESYDR-RYLEKVQSKYFSHVCTAPVEADED--------------LSIVTG 491
           T++       +  D  RY E +  K FSHVCT PV+ D +                IVTG
Sbjct: 430 TDEHIWRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQNDVAFIVTG 489

Query: 492 AQLQVVNHGMRNILFLRLRFSSVLGATVVKQAEWEGSPGLAAKSGLISTLI------SHH 551
           AQL+V  HG +++L LRLR++ V    VV+ +   G  G + KSG+ S++       S H
Sbjct: 490 AQLEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSMPLTSGSVH 549

Query: 552 FSTVQKPPPKPADVNINSAIYPGGPPVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGAR 568
            + +QK      +V ++S ++PGGPPVP    K++KFVD +++ RGPQ +PG+W+V+G R
Sbjct: 550 HNMIQK---DKNEVVLDSGVFPGGPPVPAN-NKIVKFVDLSQLCRGPQHSPGHWLVTGVR 609

BLAST of Moc10g05480 vs. ExPASy TrEMBL
Match: A0A6J1C7Y8 (MACPF domain-containing protein At4g24290 OS=Momordica charantia OX=3673 GN=LOC111008252 PE=4 SV=1)

HSP 1 Score: 1177.9 bits (3046), Expect = 0.0e+00
Identity = 580/580 (100.00%), Postives = 580/580 (100.00%), Query Frame = 0

Query: 1   MALNFPASKAAEIAIASIGRGYDITADLRLKYCKWDLHRSRLIEIDEDVGHEIVLPGGIS 60
           MALNFPASKAAEIAIASIGRGYDITADLRLKYCKWDLHRSRLIEIDEDVGHEIVLPGGIS
Sbjct: 1   MALNFPASKAAEIAIASIGRGYDITADLRLKYCKWDLHRSRLIEIDEDVGHEIVLPGGIS 60

Query: 61  IPNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           IPNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD
Sbjct: 61  IPNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120

Query: 121 ASNTKNLAFDGVFITLYTVALEKSQMVLCDLVRKAVPSSWDPAALARFIETFGTHIIVGL 180
           ASNTKNLAFDGVFITLYTVALEKSQMVLCDLVRKAVPSSWDPAALARFIETFGTHIIVGL
Sbjct: 121 ASNTKNLAFDGVFITLYTVALEKSQMVLCDLVRKAVPSSWDPAALARFIETFGTHIIVGL 180

Query: 181 KMGGKDVIYMKQQHSSTLQPSEVQKRLKEMADKRFSETIGPYGMDSERIYNNDRDIATIF 240
           KMGGKDVIYMKQQHSSTLQPSEVQKRLKEMADKRFSETIGPYGMDSERIYNNDRDIATIF
Sbjct: 181 KMGGKDVIYMKQQHSSTLQPSEVQKRLKEMADKRFSETIGPYGMDSERIYNNDRDIATIF 240

Query: 241 KRRGGIESINLPHHEWLQTVQLGPDVISMSFIPITSLLNGVPGSGFLSHAINLYLRYKPP 300
           KRRGGIESINLPHHEWLQTVQLGPDVISMSFIPITSLLNGVPGSGFLSHAINLYLRYKPP
Sbjct: 241 KRRGGIESINLPHHEWLQTVQLGPDVISMSFIPITSLLNGVPGSGFLSHAINLYLRYKPP 300

Query: 301 IEELHQFLEFQLPRQWAPIFSELPLGPQRKHQNLASLQFSLMGSKLYVNTTPVDVGRRPV 360
           IEELHQFLEFQLPRQWAPIFSELPLGPQRKHQNLASLQFSLMGSKLYVNTTPVDVGRRPV
Sbjct: 301 IEELHQFLEFQLPRQWAPIFSELPLGPQRKHQNLASLQFSLMGSKLYVNTTPVDVGRRPV 360

Query: 361 TGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPNGNFHPESYDRRYLEKVQSKYFSHV 420
           TGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPNGNFHPESYDRRYLEKVQSKYFSHV
Sbjct: 361 TGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPNGNFHPESYDRRYLEKVQSKYFSHV 420

Query: 421 CTAPVEADEDLSIVTGAQLQVVNHGMRNILFLRLRFSSVLGATVVKQAEWEGSPGLAAKS 480
           CTAPVEADEDLSIVTGAQLQVVNHGMRNILFLRLRFSSVLGATVVKQAEWEGSPGLAAKS
Sbjct: 421 CTAPVEADEDLSIVTGAQLQVVNHGMRNILFLRLRFSSVLGATVVKQAEWEGSPGLAAKS 480

Query: 481 GLISTLISHHFSTVQKPPPKPADVNINSAIYPGGPPVPVQAPKLLKFVDTTEMKRGPQET 540
           GLISTLISHHFSTVQKPPPKPADVNINSAIYPGGPPVPVQAPKLLKFVDTTEMKRGPQET
Sbjct: 481 GLISTLISHHFSTVQKPPPKPADVNINSAIYPGGPPVPVQAPKLLKFVDTTEMKRGPQET 540

Query: 541 PGYWVVSGARLMVEKGRISLRVKFSLLTIILPDEEEINDN 581
           PGYWVVSGARLMVEKGRISLRVKFSLLTIILPDEEEINDN
Sbjct: 541 PGYWVVSGARLMVEKGRISLRVKFSLLTIILPDEEEINDN 580

BLAST of Moc10g05480 vs. ExPASy TrEMBL
Match: A0A0A0L5E1 (MACPF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G110020 PE=4 SV=1)

HSP 1 Score: 1087.8 bits (2812), Expect = 0.0e+00
Identity = 541/583 (92.80%), Postives = 552/583 (94.68%), Query Frame = 0

Query: 1   MALNFPASKAAEIAIASIGRGYDITADLRLKYCKWDLHRSRLIEIDEDVGHEIVLPGGIS 60
           MAL    SKAAEIAI SIGRGYDIT DLRLKYCK +LH SRLIEIDE VGHEIVLPGGI 
Sbjct: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIL 60

Query: 61  IPNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           I NVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD
Sbjct: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120

Query: 121 ASNTKNLAFDGVFITLYTVALEKSQMVLCDLVRKAVPSSWDPAALARFIETFGTHIIVGL 180
           A+NTKNLAFDGVFITLYTVALEKSQMVLCD VRKAVPSSWDPAALARFIETFGTHIIVGL
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180

Query: 181 KMGGKDVIYMKQQHSSTLQ---PSEVQKRLKEMADKRFSETIGPYGMDSERIYNNDRDIA 240
           KMGGKDVIYMKQQHSSTLQ   PSEVQK LKEMADKRFSETI  YGM SER+YNND+DI 
Sbjct: 181 KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKDIV 240

Query: 241 TIFKRRGGIESINLPHHEWLQTVQLGPDVISMSFIPITSLLNGVPGSGFLSHAINLYLRY 300
            I KRRGG + I+LPHHEWLQTVQL PDVISMSFIPITSLLNGVPGSGFLSHAINLYLRY
Sbjct: 241 PICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITSLLNGVPGSGFLSHAINLYLRY 300

Query: 301 KPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKHQNLASLQFSLMGSKLYVNTTPVDVGR 360
           KPPIEELHQFLEFQLPRQWAPIFSELPLGPQRK  NLASLQFSLMGSKLYVNTTPVDVGR
Sbjct: 301 KPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLASLQFSLMGSKLYVNTTPVDVGR 360

Query: 361 RPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPNGNFHPESYDRRYLEKVQSKYF 420
           RPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPNGNF  +SYDRRYLEKVQSKYF
Sbjct: 361 RPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPNGNFRQDSYDRRYLEKVQSKYF 420

Query: 421 SHVCTAPVEADEDLSIVTGAQLQVVNHGMRNILFLRLRFSSVLGATVVKQAEWEGSPGLA 480
           SHVCTAPVEADEDLSIVTGAQLQVVN GMRN+LFLRLRFSSVLGATVV+QAEWEGSPGLA
Sbjct: 421 SHVCTAPVEADEDLSIVTGAQLQVVNQGMRNVLFLRLRFSSVLGATVVRQAEWEGSPGLA 480

Query: 481 AKSGLISTLISHHFSTVQKPPPKPADVNINSAIYPGGPPVPVQAPKLLKFVDTTEMKRGP 540
           AKSGLISTLISHHFSTV KPPPKPADVNINSA+YPGGPPVPVQAPKLLKFVDTTEMKRGP
Sbjct: 481 AKSGLISTLISHHFSTVMKPPPKPADVNINSAVYPGGPPVPVQAPKLLKFVDTTEMKRGP 540

Query: 541 QETPGYWVVSGARLMVEKGRISLRVKFSLLTIILPDEEEINDN 581
           QETPGYWVVSGARLMVEKGRISLRVKFSLLT+ILPDEEEINDN
Sbjct: 541 QETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEEEINDN 583

BLAST of Moc10g05480 vs. ExPASy TrEMBL
Match: A0A6J1JAX4 (MACPF domain-containing protein At4g24290-like OS=Cucurbita maxima OX=3661 GN=LOC111482823 PE=4 SV=1)

HSP 1 Score: 1085.9 bits (2807), Expect = 0.0e+00
Identity = 535/580 (92.24%), Postives = 550/580 (94.83%), Query Frame = 0

Query: 1   MALNFPASKAAEIAIASIGRGYDITADLRLKYCKWDLHRSRLIEIDEDVGHEIVLPGGIS 60
           MAL    SKAAEIAI SIGRGYDIT DLRLKYCK + H SRLIEIDE VGHEIVLPGGI 
Sbjct: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNFHGSRLIEIDEGVGHEIVLPGGIL 60

Query: 61  IPNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           I NVPKSIKCDKGERTRFRSDVLSFQQMSE FNQEMTLTGKIPSGH NTMFEFSGCWQKD
Sbjct: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEHFNQEMTLTGKIPSGHLNTMFEFSGCWQKD 120

Query: 121 ASNTKNLAFDGVFITLYTVALEKSQMVLCDLVRKAVPSSWDPAALARFIETFGTHIIVGL 180
           A+NTKNLAFDGVFITLYTVALEKSQMVLCD VRK VPS+WDPAALARFIETFGTHIIVGL
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKEVPSTWDPAALARFIETFGTHIIVGL 180

Query: 181 KMGGKDVIYMKQQHSSTLQPSEVQKRLKEMADKRFSETIGPYGMDSERIYNNDRDIATIF 240
           KMGGKDVIYMKQQHSSTLQP+EVQKRLKEMADKRFSETIG YGM SE+IYNND+DI  I+
Sbjct: 181 KMGGKDVIYMKQQHSSTLQPTEVQKRLKEMADKRFSETIGQYGMGSEQIYNNDKDILAIY 240

Query: 241 KRRGGIESINLPHHEWLQTVQLGPDVISMSFIPITSLLNGVPGSGFLSHAINLYLRYKPP 300
           KRRGG + IN PHHEWLQTVQL PDVISMSFIPITSLLNGVPGSGFLSHAINLYLRYKPP
Sbjct: 241 KRRGGSDRINYPHHEWLQTVQLEPDVISMSFIPITSLLNGVPGSGFLSHAINLYLRYKPP 300

Query: 301 IEELHQFLEFQLPRQWAPIFSELPLGPQRKHQNLASLQFSLMGSKLYVNTTPVDVGRRPV 360
           IEELHQFLEFQLPRQWAPIFSELPLGPQRK  NLASLQFSLMGSKLYVNTTPVDVGRRPV
Sbjct: 301 IEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLASLQFSLMGSKLYVNTTPVDVGRRPV 360

Query: 361 TGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPNGNFHPESYDRRYLEKVQSKYFSHV 420
           TGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPNGNF  ESYDRRYLEKVQSKYFSHV
Sbjct: 361 TGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPNGNFCQESYDRRYLEKVQSKYFSHV 420

Query: 421 CTAPVEADEDLSIVTGAQLQVVNHGMRNILFLRLRFSSVLGATVVKQAEWEGSPGLAAKS 480
           CTAP+EADEDLSIVTGAQLQVVNHGM+N+LFLRLRFSSVLGATVV+Q EWEGSPGLAAKS
Sbjct: 421 CTAPIEADEDLSIVTGAQLQVVNHGMKNVLFLRLRFSSVLGATVVRQPEWEGSPGLAAKS 480

Query: 481 GLISTLISHHFSTVQKPPPKPADVNINSAIYPGGPPVPVQAPKLLKFVDTTEMKRGPQET 540
           GLISTLISHHFSTVQKPPPKPADV INSA+YPGGPPVPVQAPKLLKFVDTTEMKRGPQ+T
Sbjct: 481 GLISTLISHHFSTVQKPPPKPADVIINSAVYPGGPPVPVQAPKLLKFVDTTEMKRGPQDT 540

Query: 541 PGYWVVSGARLMVEKGRISLRVKFSLLTIILPDEEEINDN 581
           PGYWVVSGARLMVEKGRISLRVKFSLLT+ILPDEEEINDN
Sbjct: 541 PGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEEEINDN 580

BLAST of Moc10g05480 vs. ExPASy TrEMBL
Match: A0A6J1EMJ3 (MACPF domain-containing protein At4g24290-like OS=Cucurbita moschata OX=3662 GN=LOC111435883 PE=4 SV=1)

HSP 1 Score: 1085.5 bits (2806), Expect = 0.0e+00
Identity = 535/580 (92.24%), Postives = 551/580 (95.00%), Query Frame = 0

Query: 1   MALNFPASKAAEIAIASIGRGYDITADLRLKYCKWDLHRSRLIEIDEDVGHEIVLPGGIS 60
           MAL    SKAAEIAI SIGRGYDIT DLRLKYCK + H SRLIEIDE VGHEIVLPGGI 
Sbjct: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNFHGSRLIEIDEGVGHEIVLPGGIL 60

Query: 61  IPNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           I NVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGH NTMFEFSGCWQKD
Sbjct: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHLNTMFEFSGCWQKD 120

Query: 121 ASNTKNLAFDGVFITLYTVALEKSQMVLCDLVRKAVPSSWDPAALARFIETFGTHIIVGL 180
           A+NTKNLAFDGVFITLYTVALEKSQMVLCD VRKAVPS+WDPAALARFIETFGTHIIVGL
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSTWDPAALARFIETFGTHIIVGL 180

Query: 181 KMGGKDVIYMKQQHSSTLQPSEVQKRLKEMADKRFSETIGPYGMDSERIYNNDRDIATIF 240
           KMGGKDVIYMKQQH STLQPSEVQKRLKEMADKRFSETIG YGM SE+IYN+D+DI  I+
Sbjct: 181 KMGGKDVIYMKQQHYSTLQPSEVQKRLKEMADKRFSETIGQYGMGSEQIYNSDKDILAIY 240

Query: 241 KRRGGIESINLPHHEWLQTVQLGPDVISMSFIPITSLLNGVPGSGFLSHAINLYLRYKPP 300
           KRRGG + IN PHHEWLQTVQL PDVISMSFIPITSLLNGVPGSGFLSHAINLYLRYKPP
Sbjct: 241 KRRGGSDRINYPHHEWLQTVQLEPDVISMSFIPITSLLNGVPGSGFLSHAINLYLRYKPP 300

Query: 301 IEELHQFLEFQLPRQWAPIFSELPLGPQRKHQNLASLQFSLMGSKLYVNTTPVDVGRRPV 360
           IEELHQFLEFQLPRQWAPIFSELPLGPQRK  NLASLQFSLMGSKLYVNTTPVDVGRRPV
Sbjct: 301 IEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLASLQFSLMGSKLYVNTTPVDVGRRPV 360

Query: 361 TGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPNGNFHPESYDRRYLEKVQSKYFSHV 420
           TGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPNGNF  ESYDRRYLEKVQSKYFSHV
Sbjct: 361 TGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPNGNFCQESYDRRYLEKVQSKYFSHV 420

Query: 421 CTAPVEADEDLSIVTGAQLQVVNHGMRNILFLRLRFSSVLGATVVKQAEWEGSPGLAAKS 480
           CTAP+EADEDLSIVTGAQLQVVNHGM+N+LFLRLRFSSVLGATVV+Q EWEGSPGLAAKS
Sbjct: 421 CTAPIEADEDLSIVTGAQLQVVNHGMKNVLFLRLRFSSVLGATVVRQPEWEGSPGLAAKS 480

Query: 481 GLISTLISHHFSTVQKPPPKPADVNINSAIYPGGPPVPVQAPKLLKFVDTTEMKRGPQET 540
           GLISTLISHHFSTVQKPPPKPADV INSA+YPGGPPVPVQAPKLLKFVDTTEMKRGPQ+T
Sbjct: 481 GLISTLISHHFSTVQKPPPKPADVIINSAVYPGGPPVPVQAPKLLKFVDTTEMKRGPQDT 540

Query: 541 PGYWVVSGARLMVEKGRISLRVKFSLLTIILPDEEEINDN 581
           PGYWVVSGARLMVEKGRISLRV+FSLLT+ILPDEEEINDN
Sbjct: 541 PGYWVVSGARLMVEKGRISLRVRFSLLTVILPDEEEINDN 580

BLAST of Moc10g05480 vs. ExPASy TrEMBL
Match: A0A5D3BT29 (MACPF domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold18G00720 PE=4 SV=1)

HSP 1 Score: 1070.8 bits (2768), Expect = 1.9e-309
Identity = 538/604 (89.07%), Postives = 550/604 (91.06%), Query Frame = 0

Query: 1   MALNFPASKAAEIAIASIGRGYDITADLRLKYCKWDLHRSRLIEIDEDVGHEIVLPGGIS 60
           MAL    SKAAEIAI SIGRGYDIT DLRLKYCK +LH SRLIEIDE VGHEIVLPGGI 
Sbjct: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIV 60

Query: 61  IPNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           I NVP+SIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD
Sbjct: 61  ISNVPESIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120

Query: 121 ASNTKNLAFDGVFITLYTVALEKSQMVLCDLVRKAVPSSWDPAALARFIETFGTHIIVGL 180
           A+NTKNLAFDGVFITLYTVALEKSQMVLCD VRKAVPSSWDPAALARFIETFGTHIIVGL
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180

Query: 181 KMGGKDVIYMKQQHSSTLQ---PSEVQKRLKEMADKRFSETIGPYGMDSERIYNND---- 240
           KMGGKDVIYMKQQHSSTLQ   PSEVQK LKEMADKRFSETI  YGM SER+YNND    
Sbjct: 181 KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKIEI 240

Query: 241 -----------------RDIATIFKRRGGIESINLPHHEWLQTVQLGPDVISMSFIPITS 300
                            +DI  I KRRGG + I+LPHHEWLQTVQL PDVISMSFIPITS
Sbjct: 241 REQHLRFGDASTAISPLKDIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPITS 300

Query: 301 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKHQNLAS 360
           LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRK  NLAS
Sbjct: 301 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS 360

Query: 361 LQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPN 420
           LQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPN
Sbjct: 361 LQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPN 420

Query: 421 GNFHPESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNILFLRLRF 480
           GNF  +SYDRRYLEKVQSKYFSHVCTAPVEADED SIVTGAQLQVVN GMRN+LFLRLRF
Sbjct: 421 GNFRQDSYDRRYLEKVQSKYFSHVCTAPVEADEDFSIVTGAQLQVVNQGMRNVLFLRLRF 480

Query: 481 SSVLGATVVKQAEWEGSPGLAAKSGLISTLISHHFSTVQKPPPKPADVNINSAIYPGGPP 540
           SSV GATVV+QAEWEGSPGLAAKSGLISTLISHHFSTV KPPPKPADVNINSA+YPGGPP
Sbjct: 481 SSVFGATVVRQAEWEGSPGLAAKSGLISTLISHHFSTVMKPPPKPADVNINSAVYPGGPP 540

Query: 541 VPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTIILPDEEE 581
           VPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLT+ILPDEEE
Sbjct: 541 VPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEEE 600

BLAST of Moc10g05480 vs. TAIR 10
Match: AT4G24290.2 (MAC/Perforin domain-containing protein )

HSP 1 Score: 862.1 bits (2226), Expect = 2.6e-250
Identity = 429/598 (71.74%), Postives = 491/598 (82.11%), Query Frame = 0

Query: 1   MALNFPASKAAEIAIASIGRGYDITADLRLKYCKWDLHRSRLIEIDE-DVGHEIVLPGGI 60
           MAL  PASKAAE+AI SIG GYD+  DLRLKYCK     SRL++I E D   EIVLPGGI
Sbjct: 1   MALRLPASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGI 60

Query: 61  SIPNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQK 120
           SIPNV KSIKCDKGER RFRSD+L FQQM+EQFNQE++L GKIPSG FN MFEFS CWQK
Sbjct: 61  SIPNVSKSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQK 120

Query: 121 DASNTKNLAFDGVFITLYTVALEKSQMVLCDLVRKAVPSSWDPAALARFIETFGTHIIVG 180
           DA+ TKNLAFDGVFI+LY+VAL+KSQ++L + V++AVPS+WDPAALARFI+ +GTHIIV 
Sbjct: 121 DAAYTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVS 180

Query: 181 LKMGGKDVIYMKQQHSSTLQPSEVQKRLKEMADKRFSETIGPYGMDSERI---------- 240
           +KMGGKDVIY KQQHSS LQP ++QKRLKE+ADKRF E    +   SER+          
Sbjct: 181 VKMGGKDVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKE 240

Query: 241 -------------YNNDRDIATIFKRRGGIESINLPHHEWLQTVQLGPDVISMSFIPITS 300
                        Y N  D   + KRRGG ++ NL H+EWLQTVQ+ PDVISMSFIPITS
Sbjct: 241 QRLRFADTSSLGSYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITS 300

Query: 301 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKHQNLAS 360
           LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAP+FSELPLGPQRK Q+ AS
Sbjct: 301 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQSCAS 360

Query: 361 LQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPN 420
           LQFS  G KLYVNTTPVDVG+RP+TG+RLYLEG+RSN L+IHLQHLSSLPKI+QL +D N
Sbjct: 361 LQFSFFGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLEDDLN 420

Query: 421 GNFHPESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNILFLRLRF 480
            +   ES+DRRY EKV  K +SHVCT PVE+D+DLS+VTGAQL V +HG +N+LFLRL F
Sbjct: 421 RSIRQESHDRRYYEKVNWKNYSHVCTEPVESDDDLSVVTGAQLHVESHGFKNVLFLRLCF 480

Query: 481 SSVLGATVVKQAEWEGSPGLAAKSGLISTLISHHFSTVQKPPPKPADVNINSAIYPGGPP 540
           S V+GAT+VK +EW+ + G A KSGLISTLISHHF+  QKPPP+PADVNINSAIYPGGPP
Sbjct: 481 SRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPPRPADVNINSAIYPGGPP 540

Query: 541 VPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTIILPDE 575
           VP QAPKLLKFVDT+EM RGPQE+PGYWVVSGARL+VEKG+ISL+VK+SL T IL DE
Sbjct: 541 VPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKVKYSLFTPILGDE 598

BLAST of Moc10g05480 vs. TAIR 10
Match: AT1G28380.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 591.7 bits (1524), Expect = 6.6e-169
Identity = 314/603 (52.07%), Postives = 405/603 (67.16%), Query Frame = 0

Query: 7   ASKAAEIAIASIGRGYDITADLRLKYCKWDLHRSRLIEIDEDVGHEIVLPGGISIPNVPK 66
           A  AAE A++ IG GYD+ +D+R   CK     SRL+EID     +++ PGGI + NV  
Sbjct: 11  AHSAAEKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIVVNNVSS 70

Query: 67  SIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKDASNTKN 126
           SIKCDKGERTR RSD+LSF QMSE+FNQ+M L+GKIPSG FN MF FS CW KDAS+ K 
Sbjct: 71  SIKCDKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKDASSVKT 130

Query: 127 LAFDGVFITLYTVALEKSQMVLCDLVRKAVPSSWDPAALARFIETFGTHIIVGLKMGGKD 186
           LA+DG FI+LY+V + + Q+ L D V++ VPSSWD AALA FIE +GTH++VG+ MGGKD
Sbjct: 131 LAYDGWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGGKD 190

Query: 187 VIYMKQQHSSTLQPSEVQKRLKEMADKRFS----ETIGPYGMDSER-------------- 246
           VI++KQ   S  +P E+QK LK   D+RF     E+  P  + S +              
Sbjct: 191 VIHVKQMRKSNHEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPKEENLLQWGLQPF 250

Query: 247 ---------IYNNDRDIATIFKRRGGIESINLPHHEWLQTVQLGPDVISMSFIPITSLLN 306
                    ++  + +I  +  RRGG++ +   H  WL TV   P+VISM F+PITSLL+
Sbjct: 251 GTSVSSAVVMHTKNEEIMRVCIRRGGVD-LGQSHERWLSTVSQAPNVISMCFVPITSLLS 310

Query: 307 GVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKHQNLASLQF 366
           G+PG+GFLSHA+NLYLRYKPPIEELHQFLEFQLPRQWAP++ +LPLG +R  Q+  SLQF
Sbjct: 311 GLPGTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSPSLQF 370

Query: 367 SLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPNGNF 426
           SLMG KLYVNT+ VD G RPVTGLR +LEGK+ N L+IHLQHLS+ P    L+ D     
Sbjct: 371 SLMGPKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSHDDTYEP 430

Query: 427 HPESYDRRYLEKVQSKYFSHVCTAPVE-----ADEDLSIVTGAQLQVVNHGMRNILFLRL 486
             E  ++ Y   V+   FSHVCT PV+     +D+  SIVT A L+V   GMR +LFLRL
Sbjct: 431 IEEPVEKGYYVPVKWGIFSHVCTYPVQYNGARSDDTASIVTKAWLEVKGMGMRKVLFLRL 490

Query: 487 RFSSVLGATVVKQAEWEGSPGLAAKSGLISTLISHHFSTVQKPPP---KP-ADVNINSAI 546
            F S+  + V +++ W+     + KSG+ S +IS   ST   P P   KP + ++INSA+
Sbjct: 491 GF-SLDASAVTRKSCWDNLSTNSRKSGVFS-MISTRLSTGLSPNPATTKPQSKIDINSAV 550

Query: 547 YPGGPPVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTII 574
           YP GP  PV+ PKLL  VDT E+ RGP+E PGYWVV+GA+L VE G+IS++ K+SLLT+I
Sbjct: 551 YPRGPSPPVK-PKLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIKAKYSLLTVI 609

BLAST of Moc10g05480 vs. TAIR 10
Match: AT1G29690.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 464.5 bits (1194), Expect = 1.2e-130
Identity = 266/582 (45.70%), Postives = 354/582 (60.82%), Query Frame = 0

Query: 14  AIASIGRGYDITADLRLKYCKWDLHRSRLIEIDEDVGHEIVLPGGISIPNVPKSIKCDKG 73
           AI ++GRG+D+T+D+RL YCK     SRL+ I+E    ++ L  G  +PNVP  I C +G
Sbjct: 24  AIQALGRGFDVTSDVRLLYCK-GAPGSRLVRIEEGQNRDLELSHGFLLPNVPADIDCSRG 83

Query: 74  ERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKDASNTKNLAFDGVF 133
                R  V SF +M+E+FN    + G IP G FN MF ++G WQ DA++TK+LA  G F
Sbjct: 84  NSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAASTKSLALVGYF 143

Query: 134 ITLYTVALEKSQMVLCDLVRKAVPSSWDPAALARFIETFGTHIIVGLKMGGKDVIYMKQQ 193
           I LY V L K  +VL + +R+AVPSSWDPA+LA FIE +GTHI+  + +GG+DV+Y++Q 
Sbjct: 144 IPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYIRQH 203

Query: 194 HSSTLQPSEVQKRLKEMADKRFSETIGPYGMDSERIYN----NDRDIATIFKRRGGIESI 253
            SS L  SE++  + +M   RF E       +S+ I       D+DI  IF+RRGG + +
Sbjct: 204 QSSPLPVSEIENYVNDMIKHRFHEA------ESQSITGPLKYKDKDITVIFRRRGG-DDL 263

Query: 254 NLPHHEWLQTVQLGPDVISMSFIPITSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLE 313
              H  W +TV   PD+I+M+F PI SLL GVPG   L+ AI LYL YKPPIE+L  FL+
Sbjct: 264 EQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHLTRAIELYLEYKPPIEDLQYFLD 323

Query: 314 FQLPRQWAPIFSELPLGPQRKHQNLASLQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEG 373
           +Q+ R WAP  S L    QRK    +SLQFSLMG KL+++   V VGR+PVTGLRL LEG
Sbjct: 324 YQIARAWAPEQSNL----QRKEPVCSSLQFSLMGPKLFISADQVTVGRKPVTGLRLSLEG 383

Query: 374 KRSNCLSIHLQHLSSLPKIFQLTEDPNGNFH----------PESYDRRYLEKVQSKYFSH 433
            + N LSIHLQHL SLPKI Q    P+ + H          PE  D R+ E ++ K FSH
Sbjct: 384 SKQNRLSIHLQHLVSLPKILQ----PHWDSHVPIGAPKWQGPEEQDSRWFEPIKWKNFSH 443

Query: 434 VCTAPVEADE----DLS---IVTGAQLQVVNHGMRNILFLRLRFSSVLGATVVKQAEWEG 493
           V T+P+E  E    DLS   IVTGAQL V + G +N+L L+L FS V G T ++++ W+ 
Sbjct: 444 VSTSPIEHTETHIGDLSGVHIVTGAQLGVWDFGSKNVLHLKLLFSKVPGCT-IRRSVWDH 503

Query: 494 SPGLAAKSGLISTLISHHFSTVQKPPPKPADVNINSAIYPGGP-------PVPVQAPKLL 553
           +P   A SG +                            PGGP        V  Q+ KL 
Sbjct: 504 TP--VASSGRLE---------------------------PGGPSTSSSTEEVSGQSGKLA 559

Query: 554 KFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLL 568
           K VD++EM +GPQ+ PG+W+V+GA+L VEKG+I LRVK+SLL
Sbjct: 564 KIVDSSEMLKGPQDLPGHWLVTGAKLGVEKGKIVLRVKYSLL 559

BLAST of Moc10g05480 vs. TAIR 10
Match: AT1G14780.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 462.2 bits (1188), Expect = 6.0e-130
Identity = 262/617 (42.46%), Postives = 363/617 (58.83%), Query Frame = 0

Query: 12  EIAIASIGRGYDITADLRLKYCK---WDLHRSRLIEIDEDVGHEIVLPGGISIPNVPKSI 71
           E A+ S+G+G+D+TAD RLKYCK         RL+ +D+    E+ +PG     NV   I
Sbjct: 10  ETAVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADI 69

Query: 72  KCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEF-SGCWQKDASNTKNL 131
            CDKGERTRFRSD+L F +MSE FNQ  ++TGKIPSG+FN  F F SG W  DA+N K+L
Sbjct: 70  NCDKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSL 129

Query: 132 AFDGVFITLYTVALEK-SQMVLCDLVRKAVPSSWDPAALARFIETFGTHIIVGLKMGGKD 191
             D   +TL+ + +   +++ L D VR AVPSSWDP  LARFIE +GTH+I G+ +GG+D
Sbjct: 130 GLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQD 189

Query: 192 VIYMKQQHSSTLQPSEVQKRLKEMADK------------------------RFSETIGPY 251
           V+ ++Q  SS L    ++  L ++ D+                        +F E    +
Sbjct: 190 VVVVRQDKSSDLDNDLLRHHLYDLGDQLFTGSCLLSTRRLNKAYHHSHSQPKFPEAFNVF 249

Query: 252 GMDSERIYNN-----DRDIATIFKRRGGIESINLPHHEWLQTVQLGPDVISMSFIPITSL 311
                  +NN        I  I  +RGG +     H EWL TV   PD I+ +FIPITSL
Sbjct: 250 DDKQTVAFNNFSINSQNGITVICAKRGG-DGRAKSHSEWLITVPDKPDAINFNFIPITSL 309

Query: 312 LNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKHQNLAS- 371
           L  VPGSG LSHA++LYLRYKPP+ +L  FL+F  PR WAP+ ++LP G      N+AS 
Sbjct: 310 LKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAA---PNMASA 369

Query: 372 ---LQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIF--QL 431
              L  + MG KLYVNTTPV   + PVTG+R +LEGK+ N L+IHLQHL +       ++
Sbjct: 370 YPALHINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGEKI 429

Query: 432 TEDPNGNFHPESYDR-RYLEKVQSKYFSHVCTAPVEADED--------------LSIVTG 491
           T++       +  D  RY E +  K FSHVCT PV+ D +                IVTG
Sbjct: 430 TDEHIWRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQNDVAFIVTG 489

Query: 492 AQLQVVNHGMRNILFLRLRFSSVLGATVVKQAEWEGSPGLAAKSGLISTLI------SHH 551
           AQL+V  HG +++L LRLR++ V    VV+ +   G  G + KSG+ S++       S H
Sbjct: 490 AQLEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSMPLTSGSVH 549

Query: 552 FSTVQKPPPKPADVNINSAIYPGGPPVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGAR 568
            + +QK      +V ++S ++PGGPPVP    K++KFVD +++ RGPQ +PG+W+V+G R
Sbjct: 550 HNMIQK---DKNEVVLDSGVFPGGPPVPAN-NKIVKFVDLSQLCRGPQHSPGHWLVTGVR 609

BLAST of Moc10g05480 vs. TAIR 10
Match: AT4G24290.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 424.5 bits (1090), Expect = 1.4e-118
Identity = 222/335 (66.27%), Postives = 255/335 (76.12%), Query Frame = 0

Query: 1   MALNFPASKAAEIAIASIGRGYDITADLRLKYCKWDLHRSRLIEIDE-DVGHEIVLPGGI 60
           MAL  PASKAAE+AI SIG GYD+  DLRLKYCK     SRL++I E D   EIVLPGGI
Sbjct: 1   MALRLPASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGI 60

Query: 61  SIPNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQK 120
           SIPNV KSIKCDKGER RFRSD+L FQQM+EQFNQE++L GKIPSG FN MFEFS CWQK
Sbjct: 61  SIPNVSKSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQK 120

Query: 121 DASNTKNLAFDGVFITLYTVALEKSQMVLCDLVRKAVPSSWDPAALARFIETFGTHIIVG 180
           DA+ TKNLAFDGVFI+LY+VAL+KSQ++L + V++AVPS+WDPAALARFI+ +GTHIIV 
Sbjct: 121 DAAYTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVS 180

Query: 181 LKMGGKDVIYMKQQHSSTLQPSEVQKRLKEMADKRFSETIGPYGMDSERI---------- 240
           +KMGGKDVIY KQQHSS LQP ++QKRLKE+ADKRF E    +   SER+          
Sbjct: 181 VKMGGKDVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKE 240

Query: 241 -------------YNNDRDIATIFKRRGGIESINLPHHEWLQTVQLGPDVISMSFIPITS 300
                        Y N  D   + KRRGG ++ NL H+EWLQTVQ+ PDVISMSFIPITS
Sbjct: 241 QRLRFADTSSLGSYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITS 300

Query: 301 LLNGVPGSGFLSHAINLYLRYKP--PIEELHQFLE 310
           LLNGVPGSGFLSHAINLYLR KP   +  +H FLE
Sbjct: 301 LLNGVPGSGFLSHAINLYLRCKPINHLRFVHFFLE 335