Moc10g03870 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc10g03870
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionPyruvate kinase
Locationchr10: 2711168 .. 2714612 (+)
RNA-Seq ExpressionMoc10g03870
SyntenyMoc10g03870
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGAGGTGAGTGCTATGATGATTCGTGCCCGCTGCAACTTCGTTGCCAAAAACCCGGTGTTAGATGTGAAGCATTCGAGGTTCGGTGTGCCGGTTCCATGTCGTACCATTTGGTGCAATCGGAGGAGGATGAAGGGGAAATTTGGAAATGGAGTGAAGGCGGCAATGCAGGTGGGTTTGGAGGATGAGGAGAGATTGAAGAGATTGGAGGCGACATTAGGGCTCGACGTAGTGTCGGAGAGGGAGTTGAAGGAGAAAGGGTTTTTGGGGATGAGGAAGACGAAGCTCGTATGTACGATTGGGCCCGCGTGTTCTGCGATTGAGGATTTGGAGAAATTGGCACTGGAAGGCATGAACATTGCGAGACTTAATATGTGCCACAACTCGAAGGAGTGGCACAGCGATGTGATTAGGAAGATCAAGAAGTTAAATGAGGAGAAAGGATTTTGTATTTCGGTAATGATCGATACTGAAGGAAGTCAGATTCATGTGGTTGATCATGGAGCTCCTTCCTCTGTTAAAGTAGAGGTAAATGTTATATTATATCCCTGTTCTTTTAGCCGCATTTGAAGTGGTAATCAAATGAATGCAGAAATATGATACTGGGGTCACTATTTTCCTTTGTAATTTAAGAACATTGGATTTATCATTGTTCAGGATGGCTCAATCTGGTTGTTTACTGCTGAGAAATTCGAGGGCTCTCGTCCATTCACTGTTCAGGCAAGCTATGAAGGCTTCTCTGAAGGTGAACCTTGCATCTTCTTTTTCTTCTTCATTTTCTGAATGGTGATTTGTAGAAATATTTGAATCCCACAAGTTAATTGGGATATGAATTTGAGGTTGATGAGCTCGTTGTTATGTGCTTTTATGAATTCTGTCAGGTATCGAAGTGGGTGATTCAATTGTAATTGATGGGGGAATGGCAACCTTTGAAGTCATTGAAAAGATTGGGAACGATTTGAGCTGCAGGTGCACAGACCCTGGTTTATTTCTTCCTCGAGCAAAATGCAGCGTTTGGAGAGATGGGAGGCTTGTTGCGAGAAATCACGAGTCTCCCACGCTGCCAAGTAAGGTAGAAACGTCTCTCTCTGCTTCAAATCAATGTTCCTTGTGATTAATTTTTCTTTTAATCCAACAAAACAGGATATGAGGATTTAAACTTCTAACCTTAAGAAAAGAAGTAGATTTAAGTTATCGTTGAATTATGCTTATGTTGTCTCATTTTGAGTAATTTATTTGCGATAAATATTCATCGGTGTTCTGTTTTAGTTCAAATACAACGTTGGAATGTATAAATATGATATATTCAAAGATCATAAGCCTGAGATATTGCATTGTTCATTGTGGGCATTCTTATGGAGAAAATGTTAGGTTCTTTTTTTTTTTAAGTTATCCCCTTGCTGTGGGATTACAAGTTTTTACCACTATACACTATTGTGGACATAATGTAAGTTTCACTCTTTGAAATTTTCTTTTTAACAATTGGGTTGTCAACGTGGACTAGATTTTACTGCATACAATATGGTGACAAAAATGTCAGATTCATTATTAAAACGTTGTCTTCTTATCGTGTGCTGTCAACATTACAGTTTTTGTCCCCGAATGATATTGTGGACGAAAGTTAGTATTTCATTTTCAAGTTTTCTGTGGGGGTTTTGTCAGCATTACGAGTTTTTTTTCCATGTACACTATCATCTCTGTCTACGATAAAATTTATCCTTTTTTTTCGCGCATAAATCTAATTTTATTGACAACGGGTGTTTTTCTCTTGACAAGGTTTAATTTCTCATATTAATGCATTAAAATGATAAACGAATTCAGTAAATTATGTCTTCTTGGCTGATGTCAATTCTATGCGCCTTAACACTGCTTTTTGCACGGTATGTTATTTGGTTTACCGTTTTACAGGATTGGTCCGACATTGAATTTGGCATTTCGGAAGGTGTCGACTTTATTGCTCTTTCCTTTGTGAACAGTGCAGACCCTGTCAAGCATCTAAAGAACTACCTCTCAACAAAATCACCAAAGTAAGCATGGAGTCAGAAAGTCAATATTTTACCACGAATTAGGTTAACATTGAGGAATTCTTTGCACCAAAGAATTACTGGTGAAATTTTGATACCGAAACAGATGTTGCGTAGCTTCTAAAATCTAATTTAGTAATGAAGGGAAAAAATATTTTCATGCACAGATCTGTAAGAGTACTGGCTAAGATTGAAAGCTTGGAGGCTCTGCAAAATCTGGAAGAAATTATAGAGGCTTCTGATGGAATCATGGTGGCTCGAGGGGATCTTGGGGTTGAAATTCCTCTCGAGCAGATACCAGCAGTCCAAGAGCAAATAACTAATGTCTGCAGGGAACTGAACAAACCAGTGATCATAGCTTCACAACTTCTAGAGTCGATGGTCGAATATCCTACTCCAACACGTGCTGAGGTACGAGTTACACATATTTAAAACGTAACTTTTGAGTTGAAATTACTGGTGCTCTCTGAACATTGTGTTGAGTTGACAGGGTAGAATAATGATCAGTCTTCGTGTTCATCATAGGTTGCAGATGTTTCTGAGGCAGTTAGACAGTATGCTGATGCATTGATGCTATCTGGGGAGTCTGCCATAGGATCATATGGACAGAAAGCGCTTTCGGTTCTGCAGATGGCCAGCAGCCGAATGGAGTTGTGGAGTCGTGAAGAAAACAGAAAAAATGTTCTTCCCAAGCTTCAACTTGGGGAATCATTGCACGATCGCATTGCCGAGGAAATTTGCAACGCTGCTGCTCAATTGGGTAAAATTTACTTTTCTCCGCTCCCTTTCTTTTTCTTTTTTCCCCCTTTTTCCATGACCCGGAATCGGAGTTTACTTCCCTATGTCGGGAGCATCCGTGTCCGTCTTAAAACTCGAACTTGGGAGACATCCAAAGTTTTCTTATTAAGTTTACCTGAAGATTGGAACATGCGACCTTTGGATTGAGTAAGGTGTTACTCAGACACTGCATGTATTTGTCAATCCGGTTACACATGGATGATGGAGCTCTCCACTCCCGATCATGAAAATAAAGTCGGAACTCCCTTCTCCGTCATATCATGTTTAACTTCTCCATGTTCTCTGTGGCAGCTAACAGACTTTCAGTAGACGCCATCTTCGTGCTCACCAAGCAAGGTCACATGGCATCTCTTCTTTCCCGCAACCGCCCGAGCCCGCCTGTATTTGCATTAACCGATAACGACAGCACGAGAATGGCCTTGAATCTACAATGGGGAGTGTTTCCTCTGCGCATTGACCTCTCAGACGACATCGAGGCAAACATTTCAAGAGGCATCGAGCTTGTGAAGTCGAAGGGGCTGGTCAAACAGGGGGACTCTGTGTTGGTGGTCTCAGAAATCTCTCCAGCTCGAGCTGCATCAATGGCGACACAGTCAATCCAGCTCAAAACCATCGTGTAG

mRNA sequence

ATGGCGGAGGTGAGTGCTATGATGATTCGTGCCCGCTGCAACTTCGTTGCCAAAAACCCGGTGTTAGATGTGAAGCATTCGAGGTTCGGTGTGCCGGTTCCATGTCGTACCATTTGGTGCAATCGGAGGAGGATGAAGGGGAAATTTGGAAATGGAGTGAAGGCGGCAATGCAGGTGGGTTTGGAGGATGAGGAGAGATTGAAGAGATTGGAGGCGACATTAGGGCTCGACGTAGTGTCGGAGAGGGAGTTGAAGGAGAAAGGGTTTTTGGGGATGAGGAAGACGAAGCTCGTATGTACGATTGGGCCCGCGTGTTCTGCGATTGAGGATTTGGAGAAATTGGCACTGGAAGGCATGAACATTGCGAGACTTAATATGTGCCACAACTCGAAGGAGTGGCACAGCGATGTGATTAGGAAGATCAAGAAGTTAAATGAGGAGAAAGGATTTTGTATTTCGGTAATGATCGATACTGAAGGAAGTCAGATTCATGTGGTTGATCATGGAGCTCCTTCCTCTGTTAAAGTAGAGGATGGCTCAATCTGGTTGTTTACTGCTGAGAAATTCGAGGGCTCTCGTCCATTCACTGTTCAGGCAAGCTATGAAGGCTTCTCTGAAGGTATCGAAGTGGGTGATTCAATTGTAATTGATGGGGGAATGGCAACCTTTGAAGTCATTGAAAAGATTGGGAACGATTTGAGCTGCAGGTGCACAGACCCTGGTTTATTTCTTCCTCGAGCAAAATGCAGCGTTTGGAGAGATGGGAGGCTTGTTGCGAGAAATCACGAGTCTCCCACGCTGCCAAGTAAGGATTGGTCCGACATTGAATTTGGCATTTCGGAAGGTGTCGACTTTATTGCTCTTTCCTTTGTGAACAGTGCAGACCCTGTCAAGCATCTAAAGAACTACCTCTCAACAAAATCACCAAAATCTGTAAGAGTACTGGCTAAGATTGAAAGCTTGGAGGCTCTGCAAAATCTGGAAGAAATTATAGAGGCTTCTGATGGAATCATGGTGGCTCGAGGGGATCTTGGGGTTGAAATTCCTCTCGAGCAGATACCAGCAGTCCAAGAGCAAATAACTAATGTCTGCAGGGAACTGAACAAACCAGTGATCATAGCTTCACAACTTCTAGAGTCGATGGTCGAATATCCTACTCCAACACGTGCTGAGGTTGCAGATGTTTCTGAGGCAGTTAGACAGTATGCTGATGCATTGATGCTATCTGGGGAGTCTGCCATAGGATCATATGGACAGAAAGCGCTTTCGGTTCTGCAGATGGCCAGCAGCCGAATGGAGTTGTGGAGTCGTGAAGAAAACAGAAAAAATGTTCTTCCCAAGCTTCAACTTGGGGAATCATTGCACGATCGCATTGCCGAGGAAATTTGCAACGCTGCTGCTCAATTGGCTAACAGACTTTCAGTAGACGCCATCTTCGTGCTCACCAAGCAAGGTCACATGGCATCTCTTCTTTCCCGCAACCGCCCGAGCCCGCCTGTATTTGCATTAACCGATAACGACAGCACGAGAATGGCCTTGAATCTACAATGGGGAGTGTTTCCTCTGCGCATTGACCTCTCAGACGACATCGAGGCAAACATTTCAAGAGGCATCGAGCTTGTGAAGTCGAAGGGGCTGGTCAAACAGGGGGACTCTGTGTTGGTGGTCTCAGAAATCTCTCCAGCTCGAGCTGCATCAATGGCGACACAGTCAATCCAGCTCAAAACCATCGTGTAG

Coding sequence (CDS)

ATGGCGGAGGTGAGTGCTATGATGATTCGTGCCCGCTGCAACTTCGTTGCCAAAAACCCGGTGTTAGATGTGAAGCATTCGAGGTTCGGTGTGCCGGTTCCATGTCGTACCATTTGGTGCAATCGGAGGAGGATGAAGGGGAAATTTGGAAATGGAGTGAAGGCGGCAATGCAGGTGGGTTTGGAGGATGAGGAGAGATTGAAGAGATTGGAGGCGACATTAGGGCTCGACGTAGTGTCGGAGAGGGAGTTGAAGGAGAAAGGGTTTTTGGGGATGAGGAAGACGAAGCTCGTATGTACGATTGGGCCCGCGTGTTCTGCGATTGAGGATTTGGAGAAATTGGCACTGGAAGGCATGAACATTGCGAGACTTAATATGTGCCACAACTCGAAGGAGTGGCACAGCGATGTGATTAGGAAGATCAAGAAGTTAAATGAGGAGAAAGGATTTTGTATTTCGGTAATGATCGATACTGAAGGAAGTCAGATTCATGTGGTTGATCATGGAGCTCCTTCCTCTGTTAAAGTAGAGGATGGCTCAATCTGGTTGTTTACTGCTGAGAAATTCGAGGGCTCTCGTCCATTCACTGTTCAGGCAAGCTATGAAGGCTTCTCTGAAGGTATCGAAGTGGGTGATTCAATTGTAATTGATGGGGGAATGGCAACCTTTGAAGTCATTGAAAAGATTGGGAACGATTTGAGCTGCAGGTGCACAGACCCTGGTTTATTTCTTCCTCGAGCAAAATGCAGCGTTTGGAGAGATGGGAGGCTTGTTGCGAGAAATCACGAGTCTCCCACGCTGCCAAGTAAGGATTGGTCCGACATTGAATTTGGCATTTCGGAAGGTGTCGACTTTATTGCTCTTTCCTTTGTGAACAGTGCAGACCCTGTCAAGCATCTAAAGAACTACCTCTCAACAAAATCACCAAAATCTGTAAGAGTACTGGCTAAGATTGAAAGCTTGGAGGCTCTGCAAAATCTGGAAGAAATTATAGAGGCTTCTGATGGAATCATGGTGGCTCGAGGGGATCTTGGGGTTGAAATTCCTCTCGAGCAGATACCAGCAGTCCAAGAGCAAATAACTAATGTCTGCAGGGAACTGAACAAACCAGTGATCATAGCTTCACAACTTCTAGAGTCGATGGTCGAATATCCTACTCCAACACGTGCTGAGGTTGCAGATGTTTCTGAGGCAGTTAGACAGTATGCTGATGCATTGATGCTATCTGGGGAGTCTGCCATAGGATCATATGGACAGAAAGCGCTTTCGGTTCTGCAGATGGCCAGCAGCCGAATGGAGTTGTGGAGTCGTGAAGAAAACAGAAAAAATGTTCTTCCCAAGCTTCAACTTGGGGAATCATTGCACGATCGCATTGCCGAGGAAATTTGCAACGCTGCTGCTCAATTGGCTAACAGACTTTCAGTAGACGCCATCTTCGTGCTCACCAAGCAAGGTCACATGGCATCTCTTCTTTCCCGCAACCGCCCGAGCCCGCCTGTATTTGCATTAACCGATAACGACAGCACGAGAATGGCCTTGAATCTACAATGGGGAGTGTTTCCTCTGCGCATTGACCTCTCAGACGACATCGAGGCAAACATTTCAAGAGGCATCGAGCTTGTGAAGTCGAAGGGGCTGGTCAAACAGGGGGACTCTGTGTTGGTGGTCTCAGAAATCTCTCCAGCTCGAGCTGCATCAATGGCGACACAGTCAATCCAGCTCAAAACCATCGTGTAG

Protein sequence

MAEVSAMMIRARCNFVAKNPVLDVKHSRFGVPVPCRTIWCNRRRMKGKFGNGVKAAMQVGLEDEERLKRLEATLGLDVVSERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALEGMNIARLNMCHNSKEWHSDVIRKIKKLNEEKGFCISVMIDTEGSQIHVVDHGAPSSVKVEDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIEVGDSIVIDGGMATFEVIEKIGNDLSCRCTDPGLFLPRAKCSVWRDGRLVARNHESPTLPSKDWSDIEFGISEGVDFIALSFVNSADPVKHLKNYLSTKSPKSVRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQEQITNVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMASSRMELWSREENRKNVLPKLQLGESLHDRIAEEICNAAAQLANRLSVDAIFVLTKQGHMASLLSRNRPSPPVFALTDNDSTRMALNLQWGVFPLRIDLSDDIEANISRGIELVKSKGLVKQGDSVLVVSEISPARAASMATQSIQLKTIV
Homology
BLAST of Moc10g03870 vs. NCBI nr
Match: XP_023553283.1 (plastidial pyruvate kinase 1, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1029.6 bits (2661), Expect = 1.0e-296
Identity = 527/580 (90.86%), Postives = 551/580 (95.00%), Query Frame = 0

Query: 1   MAEVSAMMIRARCNFVAKNPVLDVKHSRFGVPVPCRTIWCNRRRMKGKFGNGVKAAMQVG 60
           MAEV+AMMIRA CNFVAKNPVLDVK S FG+PV  RT+WCNRRR  G+ GNGVKA MQV 
Sbjct: 1   MAEVNAMMIRAGCNFVAKNPVLDVKRSGFGMPVFGRTVWCNRRRTTGRVGNGVKAVMQVD 60

Query: 61  LEDEERLKR---LEATLGLDVVSERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALE 120
           LED ERL+R   LEATLGLDVV+ERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALE
Sbjct: 61  LEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALE 120

Query: 121 GMNIARLNMCHNSKEWHSDVIRKIKKLNEEKGFCISVMIDTEGSQIHVVDHGAPSSVKVE 180
           GMNIARLNMCHNS+EWH DVIRKIKKLNE KGFCISVMIDTEGSQIHVVDHGAPSS+KVE
Sbjct: 121 GMNIARLNMCHNSREWHCDVIRKIKKLNEGKGFCISVMIDTEGSQIHVVDHGAPSSIKVE 180

Query: 181 DGSIWLFTAEKFEGSRPFTVQASYEGFSEGIEVGDSIVIDGGMATFEVIEKIGNDLSCRC 240
           DGSIWLFTAEKFEGSRPFTVQASYEGFSEGI+VGDS+VIDGGMATFEVIEKIGNDLSCRC
Sbjct: 181 DGSIWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSVVIDGGMATFEVIEKIGNDLSCRC 240

Query: 241 TDPGLFLPRAKCSVWRDGRLVARNHESPTLPSKDWSDIEFGISEGVDFIALSFVNSADPV 300
           TDPGLFLPRAKCS WRDGR+V RNHESPTL SKDWSDIEFGI+EGVDFIALSFVNSADPV
Sbjct: 241 TDPGLFLPRAKCSFWRDGRVVVRNHESPTLSSKDWSDIEFGIAEGVDFIALSFVNSADPV 300

Query: 301 KHLKNYLSTKSPKSVRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQ 360
           KHLKNYLSTKS +SVRVLAKIESLE+LQNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQ
Sbjct: 301 KHLKNYLSTKSAESVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQ 360

Query: 361 EQITNVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY 420
           E+IT+VCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY
Sbjct: 361 EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY 420

Query: 421 GQKALSVLQMASSRMELWSREENRKNVLPKLQLGESLHDRIAEEICNAAAQLANRLSVDA 480
           GQKALSVLQMAS RMELWSREENR+N LP  QLG SL DRIAEEICN+AA+LANRLSVDA
Sbjct: 421 GQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLQDRIAEEICNSAAELANRLSVDA 480

Query: 481 IFVLTKQGHMASLLSRNRPSPPVFALTDNDSTRMALNLQWGVFPLRIDLSDDIEANISRG 540
           IFVLT QGHMASLLSRNRP PP+FALTD+DSTRMALNLQWGVFPLRI+LS+DIEANI RG
Sbjct: 481 IFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRG 540

Query: 541 IELVKSKGLVKQGDSVLVVSEISPARAASMATQSIQLKTI 578
           IELVKSKGLVKQGDSVLVVSEISPARAASMA+QSIQLKTI
Sbjct: 541 IELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTI 580

BLAST of Moc10g03870 vs. NCBI nr
Match: KAA0044313.1 (plastidial pyruvate kinase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 1028.5 bits (2658), Expect = 2.3e-296
Identity = 526/582 (90.38%), Postives = 551/582 (94.67%), Query Frame = 0

Query: 1   MAEVSAMMIRARCNFVAKNPVLDVKHSRFGVPV-PCRTIWCNRRRMKGKFGNGVKAAMQV 60
           MAEV+AMMIRARCNF+ +NPVLDVK   F +PV   RT+WCNRRRMKG+ GNGVKA MQV
Sbjct: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV 60

Query: 61  GLEDEERLKR---LEATLGLDVVSERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLAL 120
            L+DEERL+R   LEATLGLD+V+ERELKEKGFLGMRKTKLVCTIGPACS IEDLEKLAL
Sbjct: 61  DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL 120

Query: 121 EGMNIARLNMCHNSKEWHSDVIRKIKKLNEEKGFCISVMIDTEGSQIHVVDHGAPSSVKV 180
           EGMNIARLNMCHNS+EWH D+I+KIKKLNEEKGFCISVMID EGS+IHVVDHGAPSSVKV
Sbjct: 121 EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV 180

Query: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIEVGDSIVIDGGMATFEVIEKIGNDLSCR 240
           EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGI VGDSIVIDGGMATFEVIEKIGNDLSCR
Sbjct: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVIEKIGNDLSCR 240

Query: 241 CTDPGLFLPRAKCSVWRDGRLVARNHESPTLPSKDWSDIEFGISEGVDFIALSFVNSADP 300
           CTDPGLFLPRAKCS WRDGRLV RNHESPTL SKDWSDIEFGISEGVDFIALSFVNSADP
Sbjct: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300

Query: 301 VKHLKNYLSTKSPKSVRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360
           V+HLKNYL TKS KS+RVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV
Sbjct: 301 VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360

Query: 361 QEQITNVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420
           QE+IT VCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS
Sbjct: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420

Query: 421 YGQKALSVLQMASSRMELWSREENRKNVLPKLQLGESLHDRIAEEICNAAAQLANRLSVD 480
           YGQKALSVLQMASSRMELWSREEN KN LP+ QLG SLHDRIAEEICN+AA+LANRLSVD
Sbjct: 421 YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480

Query: 481 AIFVLTKQGHMASLLSRNRPSPPVFALTDNDSTRMALNLQWGVFPLRIDLSDDIEANISR 540
           AIFVLT QGHMASLLSRNRP PP+FALTD+DSTRMALNLQWGVFPLRIDLS+DIEANISR
Sbjct: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540

Query: 541 GIELVKSKGLVKQGDSVLVVSEISPARAASMATQSIQLKTIV 579
           GIE+VKSKGLVKQGDSVLVVSEISPARA SMA+QSIQLKTIV
Sbjct: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTIV 582

BLAST of Moc10g03870 vs. NCBI nr
Match: KAG7014927.1 (Plastidial pyruvate kinase 1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1028.1 bits (2657), Expect = 2.9e-296
Identity = 529/581 (91.05%), Postives = 551/581 (94.84%), Query Frame = 0

Query: 1   MAEVSAMMIRARCNFVAKNPVLDVKHSRFGVPVPCRTIWCNRRRMKGKFGNGVKAAMQVG 60
           MAEV+AMMIRA CNFVAKNPVLDVK S FG+PV  RT+WCN RR  G+ GNGVKA MQV 
Sbjct: 1   MAEVNAMMIRAGCNFVAKNPVLDVKRSGFGMPVFGRTVWCN-RRTTGRVGNGVKAVMQVD 60

Query: 61  LEDEERLKR---LEATLGLDVVSERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALE 120
           LED ERL+R   LEATLGLDVV+ERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALE
Sbjct: 61  LEDAERLRRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALE 120

Query: 121 GMNIARLNMCHNSKEWHSDVIRKIKKLNEEKGFCISVMIDTEGSQIHVVDHGAPSSVKVE 180
           GMNIARLNMCHNS+EWH DVIRKIKKLNE KGFCISVMIDTEGSQ+HVVDHGAPSS+KVE
Sbjct: 121 GMNIARLNMCHNSREWHRDVIRKIKKLNEGKGFCISVMIDTEGSQVHVVDHGAPSSIKVE 180

Query: 181 DGSIWLFTAEKFEGSRPFTVQASYEGFSEGIEVGDSIVIDGGMATFEVIEKIGNDLSCRC 240
           DGSIWLFTAEKFEGSRPFTVQASYEGFSEGI+VGDSIVIDGGMATFEVIEKIGNDLSCRC
Sbjct: 181 DGSIWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMATFEVIEKIGNDLSCRC 240

Query: 241 TDPGLFLPRAKCSVWRDGRLVARNHESPTLPSKDWSDIEFGISEGVDFIALSFVNSADPV 300
           TDPGLFLPRAKCS WRDGR+V RNHESPTL SKDWSDIEFGI EGVDFIALSFVNSADPV
Sbjct: 241 TDPGLFLPRAKCSFWRDGRVVVRNHESPTLSSKDWSDIEFGIVEGVDFIALSFVNSADPV 300

Query: 301 KHLKNYLSTKSPKSVRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQ 360
           KHLKNYLSTKS KSVRVLAKIESLE+LQNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQ
Sbjct: 301 KHLKNYLSTKSAKSVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQ 360

Query: 361 EQITNVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY 420
           E+IT+VCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY
Sbjct: 361 EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY 420

Query: 421 GQKALSVLQMASSRMELWSREENRKNVLPKLQLGESLHDRIAEEICNAAAQLANRLSVDA 480
           GQKALSVLQMAS RMELWSREENR+N LP  QLG SLHDRIAEEICN+AA+LANRLSVDA
Sbjct: 421 GQKALSVLQMASCRMELWSREENRENFLPHYQLGVSLHDRIAEEICNSAAELANRLSVDA 480

Query: 481 IFVLTKQGHMASLLSRNRPSPPVFALTDNDSTRMALNLQWGVFPLRIDLSDDIEANISRG 540
           IFVLT QGHMASLLSRNRP PP+FALTD+DSTRMALNLQWGVFPLRI+LS+DIEANI RG
Sbjct: 481 IFVLTTQGHMASLLSRNRPRPPIFALTDDDSTRMALNLQWGVFPLRIELSEDIEANIWRG 540

Query: 541 IELVKSKGLVKQGDSVLVVSEISPARAASMATQSIQLKTIV 579
           IELVKSKGLVKQGDSVLVVSEISPARAASMA+QSIQLKTIV
Sbjct: 541 IELVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTIV 580

BLAST of Moc10g03870 vs. NCBI nr
Match: XP_008454424.1 (PREDICTED: plastidial pyruvate kinase 1, chloroplastic-like [Cucumis melo] >ADN33819.1 pyruvate kinase [Cucumis melo subsp. melo] >TYK29442.1 plastidial pyruvate kinase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 1026.5 bits (2653), Expect = 8.6e-296
Identity = 525/582 (90.21%), Postives = 550/582 (94.50%), Query Frame = 0

Query: 1   MAEVSAMMIRARCNFVAKNPVLDVKHSRFGVPV-PCRTIWCNRRRMKGKFGNGVKAAMQV 60
           MAEV+AMMIRARCNF+ +NPVLDVK   F +PV   RT+WCNRRRMKG+ GNGVKA MQV
Sbjct: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV 60

Query: 61  GLEDEERLKR---LEATLGLDVVSERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLAL 120
            L+DEERL+R   LEATLGLD+V+ERELKEKGFLGMRKTKLVCTIGPACS IEDLEKLAL
Sbjct: 61  DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL 120

Query: 121 EGMNIARLNMCHNSKEWHSDVIRKIKKLNEEKGFCISVMIDTEGSQIHVVDHGAPSSVKV 180
           EGMNIARLNMCHNS+EWH D+I+KIKKLNEEKGFCISVMID EGS+IHVVDHGAPSSVKV
Sbjct: 121 EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV 180

Query: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIEVGDSIVIDGGMATFEVIEKIGNDLSCR 240
           EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGI VGDSIVIDGGMATFEV EKIGNDLSCR
Sbjct: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVTEKIGNDLSCR 240

Query: 241 CTDPGLFLPRAKCSVWRDGRLVARNHESPTLPSKDWSDIEFGISEGVDFIALSFVNSADP 300
           CTDPGLFLPRAKCS WRDGRLV RNHESPTL SKDWSDIEFGISEGVDFIALSFVNSADP
Sbjct: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300

Query: 301 VKHLKNYLSTKSPKSVRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360
           V+HLKNYL TKS KS+RVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV
Sbjct: 301 VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360

Query: 361 QEQITNVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420
           QE+IT VCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS
Sbjct: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420

Query: 421 YGQKALSVLQMASSRMELWSREENRKNVLPKLQLGESLHDRIAEEICNAAAQLANRLSVD 480
           YGQKALSVLQMASSRMELWSREEN KN LP+ QLG SLHDRIAEEICN+AA+LANRLSVD
Sbjct: 421 YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480

Query: 481 AIFVLTKQGHMASLLSRNRPSPPVFALTDNDSTRMALNLQWGVFPLRIDLSDDIEANISR 540
           AIFVLT QGHMASLLSRNRP PP+FALTD+DSTRMALNLQWGVFPLRIDLS+DIEANISR
Sbjct: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540

Query: 541 GIELVKSKGLVKQGDSVLVVSEISPARAASMATQSIQLKTIV 579
           GIE+VKSKGLVKQGDSVLVVSEISPARA SMA+QSIQLKTIV
Sbjct: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTIV 582

BLAST of Moc10g03870 vs. NCBI nr
Match: XP_038905899.1 (plastidial pyruvate kinase 1, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 1026.5 bits (2653), Expect = 8.6e-296
Identity = 527/581 (90.71%), Postives = 554/581 (95.35%), Query Frame = 0

Query: 1   MAEVSAMMIRARCNFVAKNPVLDVKHSRFGVPVPCRTIWCNRRRMKGKFGNGVKAAMQVG 60
           MAEV+A+MIR+R +F+AK+PVLDVK S FG+PV  RT+WCNRRR +G  GNGVKA MQV 
Sbjct: 1   MAEVNAIMIRSRFDFIAKSPVLDVKRSGFGMPVIGRTVWCNRRRTRGGVGNGVKATMQVD 60

Query: 61  LEDEERLKR---LEATLGLDVVSERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALE 120
           LEDEERLKR   LEATLGLDVV+ERELKEKGFLGMRKTKLVCTIGPAC+AIEDLEKLALE
Sbjct: 61  LEDEERLKRLEGLEATLGLDVVAERELKEKGFLGMRKTKLVCTIGPACNAIEDLEKLALE 120

Query: 121 GMNIARLNMCHNSKEWHSDVIRKIKKLNEEKGFCISVMIDTEGSQIHVVDHGAPSSVKVE 180
           GMNIARLNMCHNS+EWH DVIRKIKKLNE+KGFCISVMIDTEGSQIHVVDHGAPSSVKVE
Sbjct: 121 GMNIARLNMCHNSREWHCDVIRKIKKLNEKKGFCISVMIDTEGSQIHVVDHGAPSSVKVE 180

Query: 181 DGSIWLFTAEKFEGSRPFTVQASYEGFSEGIEVGDSIVIDGGMATFEVIEKIGNDLSCRC 240
           DGSIWLFTAEKFEGSRPFTVQASYEGFSEGI+VGDSIVIDGGMATFEVIEKIGNDLSCRC
Sbjct: 181 DGSIWLFTAEKFEGSRPFTVQASYEGFSEGIQVGDSIVIDGGMATFEVIEKIGNDLSCRC 240

Query: 241 TDPGLFLPRAKCSVWRDGRLVARNHESPTLPSKDWSDIEFGISEGVDFIALSFVNSADPV 300
           TDPGLFLPRAKCS WRDGRLV RNHESPTL SKDWSDI+FGISEGVDFIALSFVNSADPV
Sbjct: 241 TDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIKFGISEGVDFIALSFVNSADPV 300

Query: 301 KHLKNYLSTKSPKSVRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQ 360
           +HLKNYL TKS KSVRVLAKIESLE+LQNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQ
Sbjct: 301 QHLKNYLLTKSAKSVRVLAKIESLESLQNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQ 360

Query: 361 EQITNVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY 420
           E+IT+VCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY
Sbjct: 361 EEITSVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSY 420

Query: 421 GQKALSVLQMASSRMELWSREENRKNVLPKLQLGESLHDRIAEEICNAAAQLANRLSVDA 480
           GQKALSVLQMASSRME WSREENRKN LP+ QLG SLHDRIAEEICN+AA+LANRLSVDA
Sbjct: 421 GQKALSVLQMASSRMESWSREENRKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVDA 480

Query: 481 IFVLTKQGHMASLLSRNRPSPPVFALTDNDSTRMALNLQWGVFPLRIDLSDDIEANISRG 540
           IFVLT QGHMASLLSRNRPSP +FALTD+DSTRMALNLQWGV PLRID S+DIEANISRG
Sbjct: 481 IFVLTNQGHMASLLSRNRPSPLIFALTDDDSTRMALNLQWGVVPLRIDFSEDIEANISRG 540

Query: 541 IELVKSKGLVKQGDSVLVVSEISPARAASMATQSIQLKTIV 579
           IELVKSKGLVK+GDSVLVVSEISPARAASMA+QSIQLKTIV
Sbjct: 541 IELVKSKGLVKKGDSVLVVSEISPARAASMASQSIQLKTIV 581

BLAST of Moc10g03870 vs. ExPASy Swiss-Prot
Match: Q43117 (Pyruvate kinase isozyme A, chloroplastic OS=Ricinus communis OX=3988 PE=1 SV=1)

HSP 1 Score: 600.9 bits (1548), Expect = 1.5e-170
Identity = 289/491 (58.86%), Postives = 378/491 (76.99%), Query Frame = 0

Query: 72  ATLGLDVVSERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALEGMNIARLNMCHNSK 131
           +++ +D V+E ELKE GF   R+TKLVCTIGPA    E+LE LA+ GMN+AR+NMCH ++
Sbjct: 83  SSIEVDAVTETELKENGFRSTRRTKLVCTIGPATCGFEELEALAVGGMNVARINMCHGTR 142

Query: 132 EWHSDVIRKIKKLNEEKGFCISVMIDTEGSQIHVVDHGAPSSVKVEDGSIWLFTAEKFEG 191
           EWH  VI ++++LNEEKGF +++M+DTEGS+IH+ D G  SS K EDG IW F+   ++ 
Sbjct: 143 EWHKSVIERVRRLNEEKGFAVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAYDS 202

Query: 192 SRP-FTVQASYEGFSEGIEVGDSIVIDGGMATFEVIEKIGNDLSCRCTDPGLFLPRAKCS 251
            RP  T+  +Y+GF+E ++VGD +++DGGM  FEVIEKIG D+ CRCTDPGL LPRA  +
Sbjct: 203 PRPERTINVNYDGFAEDVKVGDELLVDGGMVRFEVIEKIGPDVKCRCTDPGLLLPRANLT 262

Query: 252 VWRDGRLV-ARNHESPTLPSKDWSDIEFGISEGVDFIALSFVNSADPVKHLKNYLSTKSP 311
            WRDG LV  RN   PT+ SKDW DI+FGI+EGVDFIA+SFV SA+ + HLK+Y++ +S 
Sbjct: 263 FWRDGSLVRERNAMLPTISSKDWLDIDFGIAEGVDFIAISFVKSAEVINHLKSYIAARSR 322

Query: 312 KS-VRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQEQITNVCRELN 371
            S + V+AKIES+++L+NLEEII ASDG MVARGDLG +IPLEQ+P+ Q+ I  VCR+LN
Sbjct: 323 DSDIAVIAKIESIDSLKNLEEIIRASDGAMVARGDLGAQIPLEQVPSAQQNIVQVCRQLN 382

Query: 372 KPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMA 431
           KPVI+ASQLLESM+EYPTPTRAEVADVSEAVRQ ADALMLSGESA+G Y +KAL+VL+  
Sbjct: 383 KPVIVASQLLESMIEYPTPTRAEVADVSEAVRQRADALMLSGESAMGQYPEKALAVLRSV 442

Query: 432 SSRMELWSREENRKNVLPKLQLGESLHDRIAEEICNAAAQLANRLSVDAIFVLTKQGHMA 491
           S R+E W REE     +    +G +  D I+EEICN+AA++AN L VDA+FV TK GHMA
Sbjct: 443 SVRIEKWWREEKHHEAMELPAIGSTYSDSISEEICNSAAKMANNLGVDALFVYTKDGHMA 502

Query: 492 SLLSRNRPSPPVFALTDNDSTRMALNLQWGVFPLRIDLSDDIEANISRGIELVKSKGLVK 551
           SLLSR RP  P+FA T   S R  LNLQWG+ P R+  +DD+E+N+++   L+K++G++K
Sbjct: 503 SLLSRCRPDCPIFAFTTTTSVRRRLNLQWGLIPFRLSFADDMESNLNKTFSLLKARGMIK 562

Query: 552 QGDSVLVVSEI 560
            GD V+ VS++
Sbjct: 563 SGDLVIAVSDM 573

BLAST of Moc10g03870 vs. ExPASy Swiss-Prot
Match: Q9LIK0 (Plastidial pyruvate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PKP1 PE=1 SV=1)

HSP 1 Score: 599.0 bits (1543), Expect = 5.8e-170
Identity = 292/487 (59.96%), Postives = 372/487 (76.39%), Query Frame = 0

Query: 76  LDVVSERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALEGMNIARLNMCHNSKEWHS 135
           +D V+E ELKE GF   R+TKL+CTIGPA    E LE LA+ GMN+ARLNMCH +++WH 
Sbjct: 100 VDTVTEAELKENGFRSTRRTKLICTIGPATCGFEQLEALAVGGMNVARLNMCHGTRDWHR 159

Query: 136 DVIRKIKKLNEEKGFCISVMIDTEGSQIHVVDHGAPSSVKVEDGSIWLFTAEKFEGSRP- 195
            VIR +++LNEEKGF +++M+DTEGS+IH+ D G  +S K EDG +W FT   F+ SRP 
Sbjct: 160 GVIRSVRRLNEEKGFAVAIMMDTEGSEIHMGDLGGEASAKAEDGEVWTFTVRAFDSSRPE 219

Query: 196 FTVQASYEGFSEGIEVGDSIVIDGGMATFEVIEKIGNDLSCRCTDPGLFLPRAKCSVWRD 255
            T+  SY+GF+E + VGD +++DGGM  FEVIEKIG D+ C CTDPGL LPRA  + WRD
Sbjct: 220 RTISVSYDGFAEDVRVGDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRD 279

Query: 256 GRLV-ARNHESPTLPSKDWSDIEFGISEGVDFIALSFVNSADPVKHLKNYLSTKS-PKSV 315
           G LV  RN   PT+ SKDW DI+FGI+EGVDFIA+SFV SA+ + HLK+YL+ +S    +
Sbjct: 280 GSLVRERNAMLPTISSKDWLDIDFGIAEGVDFIAVSFVKSAEVINHLKSYLAARSRGGEI 339

Query: 316 RVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQEQITNVCRELNKPVI 375
            V+AKIES+++L NLEEII ASDG MVARGDLG +IPLEQ+PA Q++I  VCR LNKPVI
Sbjct: 340 GVIAKIESIDSLTNLEEIILASDGAMVARGDLGAQIPLEQVPAAQQRIVQVCRALNKPVI 399

Query: 376 IASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMASSRM 435
           +ASQLLESM+EYPTPTRAEVADVSEAVRQ +DALMLSGESA+G +  KAL+VL+  S R+
Sbjct: 400 VASQLLESMIEYPTPTRAEVADVSEAVRQRSDALMLSGESAMGQFPDKALTVLRTVSLRI 459

Query: 436 ELWSREENRKNVLPKLQLGESLHDRIAEEICNAAAQLANRLSVDAIFVLTKQGHMASLLS 495
           E W REE R   +P   +G S  D+I+EEICN+AA++AN L VDA+FV T  GHMASL+S
Sbjct: 460 ERWWREEKRHESVPLQAIGSSFSDKISEEICNSAAKMANNLGVDAVFVYTTSGHMASLVS 519

Query: 496 RNRPSPPVFALTDNDSTRMALNLQWGVFPLRIDLSDDIEANISRGIELVKSKGLVKQGDS 555
           R RP  P+FA T   S R  LNLQWG+ P R+  SDD+E+N+++   L+KS+G++K GD 
Sbjct: 520 RCRPDCPIFAFTTTTSVRRRLNLQWGLIPFRLSFSDDMESNLNKTFSLLKSRGMIKSGDL 579

Query: 556 VLVVSEI 560
           V+ VS++
Sbjct: 580 VIAVSDM 586

BLAST of Moc10g03870 vs. ExPASy Swiss-Prot
Match: Q40545 (Pyruvate kinase isozyme A, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 586.3 bits (1510), Expect = 3.9e-166
Identity = 284/491 (57.84%), Postives = 376/491 (76.58%), Query Frame = 0

Query: 72  ATLGLDVVSERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALEGMNIARLNMCHNSK 131
           +++ +D V+E ELKE GF   R+TKL+CTIGPA    E LE+LA  GMN+AR+NMCH ++
Sbjct: 93  SSIEVDTVTEAELKENGFRSTRRTKLICTIGPATCGFEQLERLAEGGMNVARINMCHGTR 152

Query: 132 EWHSDVIRKIKKLNEEKGFCISVMIDTEGSQIHVVDHGAPSSVKVEDGSIWLFTAEKFEG 191
           EWH  VI ++++LNEEKGF +++M+DTEGS+IH+ D G  SS K EDG IW FT   F+ 
Sbjct: 153 EWHRMVIERLRRLNEEKGFAVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWNFTVRSFDP 212

Query: 192 SRP-FTVQASYEGFSEGIEVGDSIVIDGGMATFEVIEKIGNDLSCRCTDPGLFLPRAKCS 251
             P  TV  +Y+GF+E ++VGD +++DGGM  FEVIEKIG D+ C CTDPGL LPRA  +
Sbjct: 213 PLPERTVTVNYDGFAEDVKVGDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLT 272

Query: 252 VWRDGRLV-ARNHESPTLPSKDWSDIEFGISEGVDFIALSFVNSADPVKHLKNYLSTKSP 311
            WRDG+LV  RN   PT+ SKDW DI+FGI+EGVDFIA+SFV SA+ +KHLK+Y+  ++ 
Sbjct: 273 FWRDGKLVRERNAMLPTISSKDWLDIDFGIAEGVDFIAVSFVKSAEVIKHLKSYIQARAR 332

Query: 312 KS-VRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQEQITNVCRELN 371
            S + V+AKIES+++L+NLEEII+ASDG MVARGDLG +IPLEQ+P+ Q++I  +CR+LN
Sbjct: 333 DSDISVIAKIESIDSLKNLEEIIQASDGAMVARGDLGAQIPLEQVPSEQQKIVQICRQLN 392

Query: 372 KPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMA 431
           +PVI+ASQLLESM+EYP PTRAEVADVSEAVRQ  DALMLSGESA+G + +KAL+VL+  
Sbjct: 393 RPVIVASQLLESMIEYPIPTRAEVADVSEAVRQRGDALMLSGESAMGQFPEKALTVLRSV 452

Query: 432 SSRMELWSREENRKNVLPKLQLGESLHDRIAEEICNAAAQLANRLSVDAIFVLTKQGHMA 491
           S R+E   RE+ R  V+    +  S  D I+EEICN+AA++AN L VDA+FV TK GHMA
Sbjct: 453 SLRIERMWREQKRHEVIELPSIASSFSDSISEEICNSAAKMANNLEVDALFVYTKNGHMA 512

Query: 492 SLLSRNRPSPPVFALTDNDSTRMALNLQWGVFPLRIDLSDDIEANISRGIELVKSKGLVK 551
           SLLSR RP  P+FA T   S R  LNLQWG+ P R+  SDD+E+N+++   L+K++G++K
Sbjct: 513 SLLSRCRPDCPIFAFTTTTSVRRRLNLQWGLMPFRLSFSDDMESNLNKTFSLLKARGMIK 572

Query: 552 QGDSVLVVSEI 560
            GD ++ VS++
Sbjct: 573 SGDLIIAVSDM 583

BLAST of Moc10g03870 vs. ExPASy Swiss-Prot
Match: Q40546 (Pyruvate kinase isozyme G, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 322.4 bits (825), Expect = 1.0e-86
Identity = 194/489 (39.67%), Postives = 284/489 (58.08%), Query Frame = 0

Query: 93  RKTKLVCTIGPACSAIEDLEKLALEGMNIARLNMCHNSKEWHSDVIRKIKKLNEE-KGFC 152
           RKTK+VCTIGP+ S+ E + KLA  GMN+ARLNM H     H   I  +K+ N + +   
Sbjct: 91  RKTKIVCTIGPSTSSREMIWKLAEAGMNVARLNMSHGDHASHQRTIDLVKEYNAQFEDKV 150

Query: 153 ISVMIDTEGSQIHVVDHGAPSSVKVEDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIEVG 212
           I++M+DT+G +  V+    P  + +++G  + F+ ++   +   TV  +Y+ F   +E G
Sbjct: 151 IAIMLDTKGPE--VISGDVPKPILLKEGQEFNFSIKRGVSTED-TVSVNYDDFINDVEAG 210

Query: 213 DSIVIDGGMATFEVIEKIGNDLSCRCTDPGLFLPRAKCSVWRDGRLVARNHESPTLPS-- 272
           D +++DGGM +  V  K  + + C   D G    R   +V           +S TLPS  
Sbjct: 211 DILLVDGGMMSLAVKSKTSDIVKCEVIDGGELKSRRHLNV---------RGKSATLPSIT 270

Query: 273 -KDWSDIEFGISEGVDFIALSFVNSADPVKHLKNYLSTKSPKSVRVLAKIESLEALQNLE 332
            KDW DI+FG++  VDF A+SFV  A  V  LK+YL + +   + V+ KIES +++ NL 
Sbjct: 271 EKDWDDIKFGVNNQVDFYAVSFVKDAKVVHELKDYLKSCN-ADIHVIVKIESADSIPNLH 330

Query: 333 EIIEASDGIMVARGDLGVEIPLEQIPAVQEQITNVCRELNKPVIIASQLLESMVEYPTPT 392
            II ASDG MVARGDLG E+P+E++P +QE I   C+ + KPVI+A+ +LESM+++PTPT
Sbjct: 331 SIISASDGAMVARGDLGAELPIEEVPLLQEDIIRRCQSMQKPVIVATNMLESMIDHPTPT 390

Query: 393 RAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMASSRMELWSREENRKNVLPKL 452
           RAEV+D+S AVR+ ADA+MLSGE+A G Y  KA+ V+ + + R E       +    P  
Sbjct: 391 RAEVSDISIAVREGADAVMLSGETAHGKYPLKAVKVMHIVALRTE---SSLQKSTSSPSQ 450

Query: 453 QLGESLHDRIAEEICNAAAQLANRLSVDAIFVLTKQGHMASLLSRNRPSPPVFALTDNDS 512
                 H  + E     ++ +AN LS   I V T+ G MA +LS NRPS  VFA T+N+ 
Sbjct: 451 SAAYKSH--MGEMFAFHSSSMANTLST-PIIVFTRTGSMAIILSHNRPSSTVFAFTNNER 510

Query: 513 TRMALNLQWGVFPLRIDLSDDIEANISRGIELVKSKGLVKQGDSVLVVSEISPARAASMA 572
            +  L L  GV P+ ++ S D E   SR I+L+ SK LVK G  V +V   +       +
Sbjct: 511 VKQRLALYHGVVPIYMEFSSDAEETFSRAIKLLLSKSLVKDGQYVTLVQSGAQPIWRRHS 560

Query: 573 TQSIQLKTI 578
           T  IQ++ +
Sbjct: 571 THHIQVRKV 560

BLAST of Moc10g03870 vs. ExPASy Swiss-Prot
Match: Q9FLW9 (Plastidial pyruvate kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PKP2 PE=1 SV=1)

HSP 1 Score: 320.9 bits (821), Expect = 3.0e-86
Identity = 187/489 (38.24%), Postives = 282/489 (57.67%), Query Frame = 0

Query: 93  RKTKLVCTIGPACSAIEDLEKLALEGMNIARLNMCHNSKEWHSDVIRKIKKLN-EEKGFC 152
           RKTK+VCT+GP+ +  E + KLA  GMN+AR+NM H     H  VI  +K+ N + K   
Sbjct: 110 RKTKIVCTVGPSTNTREMIWKLAEAGMNVARMNMSHGDHASHKKVIDLVKEYNAQTKDNT 169

Query: 153 ISVMIDTEGSQIHVVDHGAPSSVKVEDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIEVG 212
           I++M+DT+G ++   D   P  + ++ G  + FT E+   S P  V  +Y+ F   +E G
Sbjct: 170 IAIMLDTKGPEVRSGD--LPQPIMLDPGQEFTFTIER-GVSTPSCVSVNYDDFVNDVEAG 229

Query: 213 DSIVIDGGMATFEVIEKIGNDLSCRCTDPGLFLPRAKCSVWRDGRLVARNHESPTLPS-- 272
           D +++DGGM +F V  K  + + C   D G    R   +V           +S TLPS  
Sbjct: 230 DMLLVDGGMMSFMVKSKTKDSVKCEVVDGGELKSRRHLNV---------RGKSATLPSIT 289

Query: 273 -KDWSDIEFGISEGVDFIALSFVNSADPVKHLKNYLSTKSPKSVRVLAKIESLEALQNLE 332
            KDW DI+FG+   VDF A+SFV  A  V  LK YL   S   + V+ KIES +++ NL 
Sbjct: 290 EKDWEDIKFGVENKVDFYAVSFVKDAQVVHELKKYLQ-NSGADIHVIVKIESADSIPNLH 349

Query: 333 EIIEASDGIMVARGDLGVEIPLEQIPAVQEQITNVCRELNKPVIIASQLLESMVEYPTPT 392
            II ASDG MVARGDLG E+P+E++P +QE+I N+CR + K VI+A+ +LESM+ +PTPT
Sbjct: 350 SIITASDGAMVARGDLGAELPIEEVPILQEEIINLCRSMGKAVIVATNMLESMIVHPTPT 409

Query: 393 RAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMASSRMELWSREENRKNVLPKL 452
           RAEV+D++ AVR+ ADA+MLSGE+A G +  KA  V+   + R E           +P  
Sbjct: 410 RAEVSDIAIAVREGADAVMLSGETAHGKFPLKAAGVMHTVALRTE----ATITSGEMPP- 469

Query: 453 QLGESLHDRIAEEICNAAAQLANRLSVDAIFVLTKQGHMASLLSRNRPSPPVFALTDNDS 512
            LG++  + ++E     A  ++N L    + V T+ G MA LLS  RPS  ++A T+   
Sbjct: 470 NLGQAFKNHMSEMFAYHATMMSNTLGTSTV-VFTRTGFMAILLSHYRPSGTIYAFTNEKK 529

Query: 513 TRMALNLQWGVFPLRIDLSDDIEANISRGIELVKSKGLVKQGDSVLVVSEISPARAASMA 572
            +  L L  GV P+ ++ +DD E   +  +  +  +G+VK+G+ + +V   +     S +
Sbjct: 530 IQQRLALYQGVCPIYMEFTDDAEETFANALATLLKQGMVKKGEEIAIVQSGTQPIWRSQS 579

Query: 573 TQSIQLKTI 578
           T +IQ++ +
Sbjct: 590 THNIQVRKV 579

BLAST of Moc10g03870 vs. ExPASy TrEMBL
Match: A0A5A7TLC2 (Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G00350 PE=3 SV=1)

HSP 1 Score: 1028.5 bits (2658), Expect = 1.1e-296
Identity = 526/582 (90.38%), Postives = 551/582 (94.67%), Query Frame = 0

Query: 1   MAEVSAMMIRARCNFVAKNPVLDVKHSRFGVPV-PCRTIWCNRRRMKGKFGNGVKAAMQV 60
           MAEV+AMMIRARCNF+ +NPVLDVK   F +PV   RT+WCNRRRMKG+ GNGVKA MQV
Sbjct: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV 60

Query: 61  GLEDEERLKR---LEATLGLDVVSERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLAL 120
            L+DEERL+R   LEATLGLD+V+ERELKEKGFLGMRKTKLVCTIGPACS IEDLEKLAL
Sbjct: 61  DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL 120

Query: 121 EGMNIARLNMCHNSKEWHSDVIRKIKKLNEEKGFCISVMIDTEGSQIHVVDHGAPSSVKV 180
           EGMNIARLNMCHNS+EWH D+I+KIKKLNEEKGFCISVMID EGS+IHVVDHGAPSSVKV
Sbjct: 121 EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV 180

Query: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIEVGDSIVIDGGMATFEVIEKIGNDLSCR 240
           EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGI VGDSIVIDGGMATFEVIEKIGNDLSCR
Sbjct: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVIEKIGNDLSCR 240

Query: 241 CTDPGLFLPRAKCSVWRDGRLVARNHESPTLPSKDWSDIEFGISEGVDFIALSFVNSADP 300
           CTDPGLFLPRAKCS WRDGRLV RNHESPTL SKDWSDIEFGISEGVDFIALSFVNSADP
Sbjct: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300

Query: 301 VKHLKNYLSTKSPKSVRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360
           V+HLKNYL TKS KS+RVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV
Sbjct: 301 VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360

Query: 361 QEQITNVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420
           QE+IT VCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS
Sbjct: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420

Query: 421 YGQKALSVLQMASSRMELWSREENRKNVLPKLQLGESLHDRIAEEICNAAAQLANRLSVD 480
           YGQKALSVLQMASSRMELWSREEN KN LP+ QLG SLHDRIAEEICN+AA+LANRLSVD
Sbjct: 421 YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480

Query: 481 AIFVLTKQGHMASLLSRNRPSPPVFALTDNDSTRMALNLQWGVFPLRIDLSDDIEANISR 540
           AIFVLT QGHMASLLSRNRP PP+FALTD+DSTRMALNLQWGVFPLRIDLS+DIEANISR
Sbjct: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540

Query: 541 GIELVKSKGLVKQGDSVLVVSEISPARAASMATQSIQLKTIV 579
           GIE+VKSKGLVKQGDSVLVVSEISPARA SMA+QSIQLKTIV
Sbjct: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTIV 582

BLAST of Moc10g03870 vs. ExPASy TrEMBL
Match: A0A5D3E0J9 (Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G00350 PE=3 SV=1)

HSP 1 Score: 1026.5 bits (2653), Expect = 4.2e-296
Identity = 525/582 (90.21%), Postives = 550/582 (94.50%), Query Frame = 0

Query: 1   MAEVSAMMIRARCNFVAKNPVLDVKHSRFGVPV-PCRTIWCNRRRMKGKFGNGVKAAMQV 60
           MAEV+AMMIRARCNF+ +NPVLDVK   F +PV   RT+WCNRRRMKG+ GNGVKA MQV
Sbjct: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV 60

Query: 61  GLEDEERLKR---LEATLGLDVVSERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLAL 120
            L+DEERL+R   LEATLGLD+V+ERELKEKGFLGMRKTKLVCTIGPACS IEDLEKLAL
Sbjct: 61  DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL 120

Query: 121 EGMNIARLNMCHNSKEWHSDVIRKIKKLNEEKGFCISVMIDTEGSQIHVVDHGAPSSVKV 180
           EGMNIARLNMCHNS+EWH D+I+KIKKLNEEKGFCISVMID EGS+IHVVDHGAPSSVKV
Sbjct: 121 EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV 180

Query: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIEVGDSIVIDGGMATFEVIEKIGNDLSCR 240
           EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGI VGDSIVIDGGMATFEV EKIGNDLSCR
Sbjct: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVTEKIGNDLSCR 240

Query: 241 CTDPGLFLPRAKCSVWRDGRLVARNHESPTLPSKDWSDIEFGISEGVDFIALSFVNSADP 300
           CTDPGLFLPRAKCS WRDGRLV RNHESPTL SKDWSDIEFGISEGVDFIALSFVNSADP
Sbjct: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300

Query: 301 VKHLKNYLSTKSPKSVRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360
           V+HLKNYL TKS KS+RVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV
Sbjct: 301 VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360

Query: 361 QEQITNVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420
           QE+IT VCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS
Sbjct: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420

Query: 421 YGQKALSVLQMASSRMELWSREENRKNVLPKLQLGESLHDRIAEEICNAAAQLANRLSVD 480
           YGQKALSVLQMASSRMELWSREEN KN LP+ QLG SLHDRIAEEICN+AA+LANRLSVD
Sbjct: 421 YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480

Query: 481 AIFVLTKQGHMASLLSRNRPSPPVFALTDNDSTRMALNLQWGVFPLRIDLSDDIEANISR 540
           AIFVLT QGHMASLLSRNRP PP+FALTD+DSTRMALNLQWGVFPLRIDLS+DIEANISR
Sbjct: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540

Query: 541 GIELVKSKGLVKQGDSVLVVSEISPARAASMATQSIQLKTIV 579
           GIE+VKSKGLVKQGDSVLVVSEISPARA SMA+QSIQLKTIV
Sbjct: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTIV 582

BLAST of Moc10g03870 vs. ExPASy TrEMBL
Match: E5GBH7 (Pyruvate kinase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 1026.5 bits (2653), Expect = 4.2e-296
Identity = 525/582 (90.21%), Postives = 550/582 (94.50%), Query Frame = 0

Query: 1   MAEVSAMMIRARCNFVAKNPVLDVKHSRFGVPV-PCRTIWCNRRRMKGKFGNGVKAAMQV 60
           MAEV+AMMIRARCNF+ +NPVLDVK   F +PV   RT+WCNRRRMKG+ GNGVKA MQV
Sbjct: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV 60

Query: 61  GLEDEERLKR---LEATLGLDVVSERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLAL 120
            L+DEERL+R   LEATLGLD+V+ERELKEKGFLGMRKTKLVCTIGPACS IEDLEKLAL
Sbjct: 61  DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL 120

Query: 121 EGMNIARLNMCHNSKEWHSDVIRKIKKLNEEKGFCISVMIDTEGSQIHVVDHGAPSSVKV 180
           EGMNIARLNMCHNS+EWH D+I+KIKKLNEEKGFCISVMID EGS+IHVVDHGAPSSVKV
Sbjct: 121 EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV 180

Query: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIEVGDSIVIDGGMATFEVIEKIGNDLSCR 240
           EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGI VGDSIVIDGGMATFEV EKIGNDLSCR
Sbjct: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVTEKIGNDLSCR 240

Query: 241 CTDPGLFLPRAKCSVWRDGRLVARNHESPTLPSKDWSDIEFGISEGVDFIALSFVNSADP 300
           CTDPGLFLPRAKCS WRDGRLV RNHESPTL SKDWSDIEFGISEGVDFIALSFVNSADP
Sbjct: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300

Query: 301 VKHLKNYLSTKSPKSVRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360
           V+HLKNYL TKS KS+RVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV
Sbjct: 301 VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360

Query: 361 QEQITNVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420
           QE+IT VCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS
Sbjct: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420

Query: 421 YGQKALSVLQMASSRMELWSREENRKNVLPKLQLGESLHDRIAEEICNAAAQLANRLSVD 480
           YGQKALSVLQMASSRMELWSREEN KN LP+ QLG SLHDRIAEEICN+AA+LANRLSVD
Sbjct: 421 YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480

Query: 481 AIFVLTKQGHMASLLSRNRPSPPVFALTDNDSTRMALNLQWGVFPLRIDLSDDIEANISR 540
           AIFVLT QGHMASLLSRNRP PP+FALTD+DSTRMALNLQWGVFPLRIDLS+DIEANISR
Sbjct: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540

Query: 541 GIELVKSKGLVKQGDSVLVVSEISPARAASMATQSIQLKTIV 579
           GIE+VKSKGLVKQGDSVLVVSEISPARA SMA+QSIQLKTIV
Sbjct: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTIV 582

BLAST of Moc10g03870 vs. ExPASy TrEMBL
Match: A0A1S3BYK3 (Pyruvate kinase OS=Cucumis melo OX=3656 GN=LOC103494833 PE=3 SV=1)

HSP 1 Score: 1026.5 bits (2653), Expect = 4.2e-296
Identity = 525/582 (90.21%), Postives = 550/582 (94.50%), Query Frame = 0

Query: 1   MAEVSAMMIRARCNFVAKNPVLDVKHSRFGVPV-PCRTIWCNRRRMKGKFGNGVKAAMQV 60
           MAEV+AMMIRARCNF+ +NPVLDVK   F +PV   RT+WCNRRRMKG+ GNGVKA MQV
Sbjct: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGHRTVWCNRRRMKGRVGNGVKATMQV 60

Query: 61  GLEDEERLKR---LEATLGLDVVSERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLAL 120
            L+DEERL+R   LEATLGLD+V+ERELKEKGFLGMRKTKLVCTIGPACS IEDLEKLAL
Sbjct: 61  DLDDEERLRRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTIGPACSGIEDLEKLAL 120

Query: 121 EGMNIARLNMCHNSKEWHSDVIRKIKKLNEEKGFCISVMIDTEGSQIHVVDHGAPSSVKV 180
           EGMNIARLNMCHNS+EWH D+I+KIKKLNEEKGFCISVMID EGS+IHVVDHGAPSSVKV
Sbjct: 121 EGMNIARLNMCHNSREWHCDMIKKIKKLNEEKGFCISVMIDIEGSRIHVVDHGAPSSVKV 180

Query: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIEVGDSIVIDGGMATFEVIEKIGNDLSCR 240
           EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGI VGDSIVIDGGMATFEV EKIGNDLSCR
Sbjct: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVTEKIGNDLSCR 240

Query: 241 CTDPGLFLPRAKCSVWRDGRLVARNHESPTLPSKDWSDIEFGISEGVDFIALSFVNSADP 300
           CTDPGLFLPRAKCS WRDGRLV RNHESPTL SKDWSDIEFGISEGVDFIALSFVNSADP
Sbjct: 241 CTDPGLFLPRAKCSFWRDGRLVVRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300

Query: 301 VKHLKNYLSTKSPKSVRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360
           V+HLKNYL TKS KS+RVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV
Sbjct: 301 VRHLKNYLLTKSAKSIRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360

Query: 361 QEQITNVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420
           QE+IT VCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS
Sbjct: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420

Query: 421 YGQKALSVLQMASSRMELWSREENRKNVLPKLQLGESLHDRIAEEICNAAAQLANRLSVD 480
           YGQKALSVLQMASSRMELWSREEN KN LP+ QLG SLHDRIAEEICN+AA+LANRLSVD
Sbjct: 421 YGQKALSVLQMASSRMELWSREENTKNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480

Query: 481 AIFVLTKQGHMASLLSRNRPSPPVFALTDNDSTRMALNLQWGVFPLRIDLSDDIEANISR 540
           AIFVLT QGHMASLLSRNRP PP+FALTD+DSTRMALNLQWGVFPLRIDLS+DIEANISR
Sbjct: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540

Query: 541 GIELVKSKGLVKQGDSVLVVSEISPARAASMATQSIQLKTIV 579
           GIE+VKSKGLVKQGDSVLVVSEISPARA SMA+QSIQLKTIV
Sbjct: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAVSMASQSIQLKTIV 582

BLAST of Moc10g03870 vs. ExPASy TrEMBL
Match: A0A0A0KSY4 (Pyruvate kinase OS=Cucumis sativus OX=3659 GN=Csa_4G000560 PE=3 SV=1)

HSP 1 Score: 1025.0 bits (2649), Expect = 1.2e-295
Identity = 525/582 (90.21%), Postives = 550/582 (94.50%), Query Frame = 0

Query: 1   MAEVSAMMIRARCNFVAKNPVLDVKHSRFGVPV-PCRTIWCNRRRMKGKFGNGVKAAMQV 60
           MAEV+AMMIRARCNF+ +NPVLDVK   F +PV   RT+WC+RRRMKG+  NGVKA MQV
Sbjct: 1   MAEVNAMMIRARCNFMVRNPVLDVKRLGFRMPVMGRRTVWCSRRRMKGRVVNGVKATMQV 60

Query: 61  GLEDEERLKR---LEATLGLDVVSERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLAL 120
            LEDEERLKR   LEATLGLD+V+ERELKEKGFLGMRKTKLVCT+GPACS I DLEKLAL
Sbjct: 61  DLEDEERLKRLEGLEATLGLDIVAERELKEKGFLGMRKTKLVCTVGPACSGIADLEKLAL 120

Query: 121 EGMNIARLNMCHNSKEWHSDVIRKIKKLNEEKGFCISVMIDTEGSQIHVVDHGAPSSVKV 180
           EGMNIARLNMCHNS EWH D+I+KIKKLNEEKGFCISVMID EGSQIHVVDHGAPSSVKV
Sbjct: 121 EGMNIARLNMCHNSMEWHCDMIKKIKKLNEEKGFCISVMIDIEGSQIHVVDHGAPSSVKV 180

Query: 181 EDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIEVGDSIVIDGGMATFEVIEKIGNDLSCR 240
           EDGSIWLFTA+KFEGSRPFTVQASYEGFSEGI VGDSIVIDGGMATFEVIEKIGNDLSCR
Sbjct: 181 EDGSIWLFTAKKFEGSRPFTVQASYEGFSEGIRVGDSIVIDGGMATFEVIEKIGNDLSCR 240

Query: 241 CTDPGLFLPRAKCSVWRDGRLVARNHESPTLPSKDWSDIEFGISEGVDFIALSFVNSADP 300
           CTDPGLFLPRAKCS WRDGRLV RNHESPTL SKDWSDIEFGISEGVDFIALSFVNSADP
Sbjct: 241 CTDPGLFLPRAKCSFWRDGRLVMRNHESPTLSSKDWSDIEFGISEGVDFIALSFVNSADP 300

Query: 301 VKHLKNYLSTKSPKSVRVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAV 360
           V+HLKNYLSTKS KS+RVLAKIESLEALQNLEEI+EASDGIMVARGDLGVEIPLEQIPAV
Sbjct: 301 VRHLKNYLSTKSAKSIRVLAKIESLEALQNLEEIVEASDGIMVARGDLGVEIPLEQIPAV 360

Query: 361 QEQITNVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420
           QE+IT VCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS
Sbjct: 361 QEEITRVCRELNKPVIIASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGS 420

Query: 421 YGQKALSVLQMASSRMELWSREENRKNVLPKLQLGESLHDRIAEEICNAAAQLANRLSVD 480
           YGQKALSVLQMASSRMELWSREENR N LP+ QLG SLHDRIAEEICN+AA+LANRLSVD
Sbjct: 421 YGQKALSVLQMASSRMELWSREENRNNFLPQHQLGVSLHDRIAEEICNSAAELANRLSVD 480

Query: 481 AIFVLTKQGHMASLLSRNRPSPPVFALTDNDSTRMALNLQWGVFPLRIDLSDDIEANISR 540
           AIFVLT QGHMASLLSRNRP PP+FALTD+DSTRMALNLQWGVFPLRIDLS+DIEANISR
Sbjct: 481 AIFVLTNQGHMASLLSRNRPGPPIFALTDDDSTRMALNLQWGVFPLRIDLSEDIEANISR 540

Query: 541 GIELVKSKGLVKQGDSVLVVSEISPARAASMATQSIQLKTIV 579
           GIE+VKSKGLVKQGDSVLVVSEISPARAASMA+QSIQLKTIV
Sbjct: 541 GIEVVKSKGLVKQGDSVLVVSEISPARAASMASQSIQLKTIV 582

BLAST of Moc10g03870 vs. TAIR 10
Match: AT3G22960.1 (Pyruvate kinase family protein )

HSP 1 Score: 599.0 bits (1543), Expect = 4.1e-171
Identity = 292/487 (59.96%), Postives = 372/487 (76.39%), Query Frame = 0

Query: 76  LDVVSERELKEKGFLGMRKTKLVCTIGPACSAIEDLEKLALEGMNIARLNMCHNSKEWHS 135
           +D V+E ELKE GF   R+TKL+CTIGPA    E LE LA+ GMN+ARLNMCH +++WH 
Sbjct: 100 VDTVTEAELKENGFRSTRRTKLICTIGPATCGFEQLEALAVGGMNVARLNMCHGTRDWHR 159

Query: 136 DVIRKIKKLNEEKGFCISVMIDTEGSQIHVVDHGAPSSVKVEDGSIWLFTAEKFEGSRP- 195
            VIR +++LNEEKGF +++M+DTEGS+IH+ D G  +S K EDG +W FT   F+ SRP 
Sbjct: 160 GVIRSVRRLNEEKGFAVAIMMDTEGSEIHMGDLGGEASAKAEDGEVWTFTVRAFDSSRPE 219

Query: 196 FTVQASYEGFSEGIEVGDSIVIDGGMATFEVIEKIGNDLSCRCTDPGLFLPRAKCSVWRD 255
            T+  SY+GF+E + VGD +++DGGM  FEVIEKIG D+ C CTDPGL LPRA  + WRD
Sbjct: 220 RTISVSYDGFAEDVRVGDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRD 279

Query: 256 GRLV-ARNHESPTLPSKDWSDIEFGISEGVDFIALSFVNSADPVKHLKNYLSTKS-PKSV 315
           G LV  RN   PT+ SKDW DI+FGI+EGVDFIA+SFV SA+ + HLK+YL+ +S    +
Sbjct: 280 GSLVRERNAMLPTISSKDWLDIDFGIAEGVDFIAVSFVKSAEVINHLKSYLAARSRGGEI 339

Query: 316 RVLAKIESLEALQNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQEQITNVCRELNKPVI 375
            V+AKIES+++L NLEEII ASDG MVARGDLG +IPLEQ+PA Q++I  VCR LNKPVI
Sbjct: 340 GVIAKIESIDSLTNLEEIILASDGAMVARGDLGAQIPLEQVPAAQQRIVQVCRALNKPVI 399

Query: 376 IASQLLESMVEYPTPTRAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMASSRM 435
           +ASQLLESM+EYPTPTRAEVADVSEAVRQ +DALMLSGESA+G +  KAL+VL+  S R+
Sbjct: 400 VASQLLESMIEYPTPTRAEVADVSEAVRQRSDALMLSGESAMGQFPDKALTVLRTVSLRI 459

Query: 436 ELWSREENRKNVLPKLQLGESLHDRIAEEICNAAAQLANRLSVDAIFVLTKQGHMASLLS 495
           E W REE R   +P   +G S  D+I+EEICN+AA++AN L VDA+FV T  GHMASL+S
Sbjct: 460 ERWWREEKRHESVPLQAIGSSFSDKISEEICNSAAKMANNLGVDAVFVYTTSGHMASLVS 519

Query: 496 RNRPSPPVFALTDNDSTRMALNLQWGVFPLRIDLSDDIEANISRGIELVKSKGLVKQGDS 555
           R RP  P+FA T   S R  LNLQWG+ P R+  SDD+E+N+++   L+KS+G++K GD 
Sbjct: 520 RCRPDCPIFAFTTTTSVRRRLNLQWGLIPFRLSFSDDMESNLNKTFSLLKSRGMIKSGDL 579

Query: 556 VLVVSEI 560
           V+ VS++
Sbjct: 580 VIAVSDM 586

BLAST of Moc10g03870 vs. TAIR 10
Match: AT5G52920.1 (plastidic pyruvate kinase beta subunit 1 )

HSP 1 Score: 320.9 bits (821), Expect = 2.2e-87
Identity = 187/489 (38.24%), Postives = 282/489 (57.67%), Query Frame = 0

Query: 93  RKTKLVCTIGPACSAIEDLEKLALEGMNIARLNMCHNSKEWHSDVIRKIKKLN-EEKGFC 152
           RKTK+VCT+GP+ +  E + KLA  GMN+AR+NM H     H  VI  +K+ N + K   
Sbjct: 110 RKTKIVCTVGPSTNTREMIWKLAEAGMNVARMNMSHGDHASHKKVIDLVKEYNAQTKDNT 169

Query: 153 ISVMIDTEGSQIHVVDHGAPSSVKVEDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIEVG 212
           I++M+DT+G ++   D   P  + ++ G  + FT E+   S P  V  +Y+ F   +E G
Sbjct: 170 IAIMLDTKGPEVRSGD--LPQPIMLDPGQEFTFTIER-GVSTPSCVSVNYDDFVNDVEAG 229

Query: 213 DSIVIDGGMATFEVIEKIGNDLSCRCTDPGLFLPRAKCSVWRDGRLVARNHESPTLPS-- 272
           D +++DGGM +F V  K  + + C   D G    R   +V           +S TLPS  
Sbjct: 230 DMLLVDGGMMSFMVKSKTKDSVKCEVVDGGELKSRRHLNV---------RGKSATLPSIT 289

Query: 273 -KDWSDIEFGISEGVDFIALSFVNSADPVKHLKNYLSTKSPKSVRVLAKIESLEALQNLE 332
            KDW DI+FG+   VDF A+SFV  A  V  LK YL   S   + V+ KIES +++ NL 
Sbjct: 290 EKDWEDIKFGVENKVDFYAVSFVKDAQVVHELKKYLQ-NSGADIHVIVKIESADSIPNLH 349

Query: 333 EIIEASDGIMVARGDLGVEIPLEQIPAVQEQITNVCRELNKPVIIASQLLESMVEYPTPT 392
            II ASDG MVARGDLG E+P+E++P +QE+I N+CR + K VI+A+ +LESM+ +PTPT
Sbjct: 350 SIITASDGAMVARGDLGAELPIEEVPILQEEIINLCRSMGKAVIVATNMLESMIVHPTPT 409

Query: 393 RAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMASSRMELWSREENRKNVLPKL 452
           RAEV+D++ AVR+ ADA+MLSGE+A G +  KA  V+   + R E           +P  
Sbjct: 410 RAEVSDIAIAVREGADAVMLSGETAHGKFPLKAAGVMHTVALRTE----ATITSGEMPP- 469

Query: 453 QLGESLHDRIAEEICNAAAQLANRLSVDAIFVLTKQGHMASLLSRNRPSPPVFALTDNDS 512
            LG++  + ++E     A  ++N L    + V T+ G MA LLS  RPS  ++A T+   
Sbjct: 470 NLGQAFKNHMSEMFAYHATMMSNTLGTSTV-VFTRTGFMAILLSHYRPSGTIYAFTNEKK 529

Query: 513 TRMALNLQWGVFPLRIDLSDDIEANISRGIELVKSKGLVKQGDSVLVVSEISPARAASMA 572
            +  L L  GV P+ ++ +DD E   +  +  +  +G+VK+G+ + +V   +     S +
Sbjct: 530 IQQRLALYQGVCPIYMEFTDDAEETFANALATLLKQGMVKKGEEIAIVQSGTQPIWRSQS 579

Query: 573 TQSIQLKTI 578
           T +IQ++ +
Sbjct: 590 THNIQVRKV 579

BLAST of Moc10g03870 vs. TAIR 10
Match: AT1G32440.1 (plastidial pyruvate kinase 3 )

HSP 1 Score: 312.0 bits (798), Expect = 1.0e-84
Identity = 194/493 (39.35%), Postives = 286/493 (58.01%), Query Frame = 0

Query: 93  RKTKLVCTIGPACSAIEDLEKLALEGMNIARLNMCHNSKEWHS---DVIRKIKKLNEEKG 152
           RKTK+VCTIGP+ S+ E + KLA  GMN+ARLNM H     H    D++++   L  +K 
Sbjct: 99  RKTKIVCTIGPSSSSREMIWKLAEAGMNVARLNMSHGDHASHQITIDLVKEYNSLFVDK- 158

Query: 153 FCISVMIDTEGSQIHVVDHGAPSSVKVEDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIE 212
             I++M+DT+G ++   D   P  + +E+G  + FT ++    +  TV  +Y+ F   +E
Sbjct: 159 -AIAIMLDTKGPEVRSGD--VPQPIFLEEGQEFNFTIKRGVSLKD-TVSVNYDDFVNDVE 218

Query: 213 VGDSIVIDGGMATFEVIEKIGNDLSCRCTDPGLFLPRAKCSVWRDGRLVARNHESPTLPS 272
           VGD +++DGGM +  V  K  + + C   D G    R   +V           +S TLPS
Sbjct: 219 VGDILLVDGGMMSLAVKSKTSDLVKCVVIDGGELQSRRHLNV---------RGKSATLPS 278

Query: 273 ---KDWSDIEFGISEGVDFIALSFVNSADPVKHLKNYLSTKSPKSVRVLAKIESLEALQN 332
              KDW DI+FG+   VDF A+SFV  A  V  LKNYL T S   + V+ KIES ++++N
Sbjct: 279 ITDKDWEDIKFGVDNQVDFYAVSFVKDAKVVHELKNYLKTCS-ADISVIVKIESADSIKN 338

Query: 333 LEEIIEASDGIMVARGDLGVEIPLEQIPAVQEQITNVCRELNKPVIIASQLLESMVEYPT 392
           L  II A DG MVARGDLG E+P+E++P +QE+I   CR ++KPVI+A+ +LESM+ +PT
Sbjct: 339 LPSIISACDGAMVARGDLGAELPIEEVPLLQEEIIRRCRSIHKPVIVATNMLESMINHPT 398

Query: 393 PTRAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMASSRME--LWSREENRKNV 452
           PTRAEV+D++ AVR+ ADA+MLSGE+A G +  KA++V+   + R E  L  R    +  
Sbjct: 399 PTRAEVSDIAIAVREGADAIMLSGETAHGKFPLKAVNVMHTVALRTEASLPVRTSASRTT 458

Query: 453 LPKLQLGESLHDRIAEEICNAAAQLANRLSVDAIFVLTKQGHMASLLSRNRPSPPVFALT 512
             K  +G+             A+ +AN LS   + V T+ G MA LLS  RPS  +FA T
Sbjct: 459 AYKGHMGQM--------FAFHASIMANTLS-SPLIVFTRTGSMAVLLSHYRPSATIFAFT 518

Query: 513 DNDSTRMALNLQWGVFPLRIDLSDDIEANISRGIELVKSKGLVKQGDSVLVVSEISPARA 572
           +       L L  GV P+ ++ SDD E   +R ++L++ + ++K+G  V +V   S    
Sbjct: 519 NQRRIMQRLALYQGVMPIYMEFSDDAEDTYARSLKLLQDENMLKEGQHVTLVQSGSQPIW 567

Query: 573 ASMATQSIQLKTI 578
              +T  IQ++ I
Sbjct: 579 REESTHLIQVRKI 567

BLAST of Moc10g03870 vs. TAIR 10
Match: AT3G04050.1 (Pyruvate kinase family protein )

HSP 1 Score: 230.7 bits (587), Expect = 2.9e-60
Identity = 161/499 (32.26%), Postives = 252/499 (50.50%), Query Frame = 0

Query: 94  KTKLVCTIGPACSAIEDLEKLALEGMNIARLNMCHNSKEWHSDVIRKIKKLNEEKGFCIS 153
           KTK+VCT+GPA  ++E +EKL   GMN+AR N  H S  +H + +  ++   E      +
Sbjct: 17  KTKIVCTLGPASRSVEMVEKLLKAGMNVARFNFSHGSHSYHQETLDNLRTAMENTCIPCA 76

Query: 154 VMIDTEGSQIHV--VDHGAPSSVKVEDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIEVG 213
           VM+DT+G +I    +  G P  + ++   I + T    EG    T+  SY+  +E ++ G
Sbjct: 77  VMLDTKGPEIRTGFLKEGKPVEL-IQGQEITISTDYTMEGDSN-TISMSYKKLAEDLKSG 136

Query: 214 DSIVIDGGMATFEVIEKIGNDLSCRCTDPGLFLPRAKC------SVWRDGRLVARNHESP 273
           D I+   G  +  V       LSC   D  L L RA+C         ++  L     + P
Sbjct: 137 DVILCSDGTISLTV-------LSC---DKNLGLVRARCENSAVLGERKNVNLPGIVVDLP 196

Query: 274 TLPSKDWSDI-EFGISEGVDFIALSFVNSADPVKHLKNYLSTKSPKSVRVLAKIESLEAL 333
           TL  KD  DI ++G+   +D IALSFV     +  ++  L  ++ KS+ +++K+E+ E +
Sbjct: 197 TLTEKDQEDILQWGVPNKIDIIALSFVRKGSDLVEVRKLLG-ENAKSIMLMSKVENQEGV 256

Query: 334 QNLEEIIEASDGIMVARGDLGVEIPLEQIPAVQEQITNVCRELNKPVIIASQLLESMVEY 393
            N ++I+E SD  MVARGDLG+EIP+E++   Q+ +      L KP++ A+Q+LESM + 
Sbjct: 257 MNFDKILEYSDAFMVARGDLGMEIPIEKMFLAQKMMIQKANALGKPIVTATQMLESMTKS 316

Query: 394 PTPTRAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMASSRMELWSREENRKNV 453
           P PTRAE  DV+ AV    D +MLSGE+A G++ + A+  +       E +   +     
Sbjct: 317 PRPTRAEATDVANAVLDGTDCVMLSGETAAGAHPETAVLTMSRICKEAEDFIDYDTMHK- 376

Query: 454 LPKLQLGESLHDRIAEEICNAAAQLANRLSVDAIFVLTKQGHMASLLSRNRPSPPVFALT 513
             K+Q   SL     E +  +A   A  L   AI VLTK G+   L+++ RPS P+ ++ 
Sbjct: 377 --KIQDIVSLPLSPIESLAASAVSTARSLCAAAIVVLTKGGYTVELVAKYRPSVPILSVI 436

Query: 514 DNDSTRMALNLQW-----------------GVFPL-------RIDLSDDIEANISRGIEL 560
             + TR   + +W                 G+ P+       R    D  E  I   IE 
Sbjct: 437 VPEITRTD-DFEWSCSESAAHVARRGLIYRGIVPVMATGASARSSNKDSTEETIQFAIEF 496

BLAST of Moc10g03870 vs. TAIR 10
Match: AT3G25960.1 (Pyruvate kinase family protein )

HSP 1 Score: 229.2 bits (583), Expect = 8.6e-60
Identity = 159/482 (32.99%), Postives = 254/482 (52.70%), Query Frame = 0

Query: 94  KTKLVCTIGPACSAIEDLEKLALEGMNIARLNMCHNSKEWHSDVIRKIKKLNEEKGFCIS 153
           KTK+VCT+GPA  ++E +EKL   GMN+AR N  H S  +H + +  ++   +  G   +
Sbjct: 17  KTKIVCTLGPASRSVEMIEKLLKAGMNVARFNFSHGSHSYHQETLDNLRTAMDNTGILCA 76

Query: 154 VMIDTEGSQIHV--VDHGAPSSVKVEDGSIWLFTAEKFEGSRPFTVQASYEGFSEGIEVG 213
           VM+DT+   I    +  G P  +K +   I +    K +G    T+  SY+  +E ++ G
Sbjct: 77  VMLDTKSPVIRTGFLKEGKPIQLK-QGQEITISIDYKIQGDSN-TISMSYKKLAEDLKPG 136

Query: 214 DSIVIDGGMATFEVI--EKIGNDLSCRCTDPGLFLPRAKCSVWRDGRLVARNHESPTLPS 273
           D I+   G  +  V+  +K    + CRC +  L   R   ++   G +V    + PTL  
Sbjct: 137 DVILCSDGTISLNVLSCDKYLGLVRCRCENSALLGERKNVNL--PGIVV----DLPTLTE 196

Query: 274 KDWSDI-EFGISEGVDFIALSFVNSADPVKHLKNYLSTKSPKSVRVLAKIESLEALQNLE 333
           KD  DI ++G+   +D IALSFV     +  ++  L   S KS+ +++K+E+ E + N +
Sbjct: 197 KDKEDIMQWGVPNKIDIIALSFVRKGSDLIQVRKLLGEHS-KSIMLMSKVENQEGVMNFD 256

Query: 334 EIIEASDGIMVARGDLGVEIPLEQIPAVQEQITNVCRELNKPVIIASQLLESMVEYPTPT 393
           +I+E SD  MVARGDLG+EIP+E++   Q+ + N      KPV+ A+Q+LESM   P PT
Sbjct: 257 KILENSDAFMVARGDLGMEIPIEKMFLAQKTMINKANAHGKPVVTATQMLESMTVSPRPT 316

Query: 394 RAEVADVSEAVRQYADALMLSGESAIGSYGQKALSVLQMASSRMELWSREENRKNVLPKL 453
           RAE  DV+ AV    D +MLSGE+A G++ + A+    +  SR+   + +    ++L K 
Sbjct: 317 RAEATDVANAVLDGTDCVMLSGETAAGAHPETAV----LTMSRICKEAEDFIDYDILHKK 376

Query: 454 QLG-ESLHDRIAEEICNAAAQLANRLSVDAIFVLTKQGHMASLLSRNRPSPPVFAL---- 513
            LG  SL     E +  +A   A  +   AI VLT+ G+ A L+++ RPS P+ ++    
Sbjct: 377 TLGMVSLPLSPIESLAASAVSTARSVFASAIVVLTRGGYTAELVAKYRPSVPILSVIMPE 436

Query: 514 --TDNDSTRMALN---LQWGVFP-LRIDLSDDIEANISRGIELVKSKGLVKQGDSVLVVS 560
               +DS         +  G+ P +     D  E  I   I   K+KG+ K GDS++ + 
Sbjct: 437 IAECSDSVAHVARRGLIYRGIIPVVGCSARDSTEEMIRLAIGFAKTKGICKTGDSIVALH 485

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023553283.11.0e-29690.86plastidial pyruvate kinase 1, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
KAA0044313.12.3e-29690.38plastidial pyruvate kinase 1 [Cucumis melo var. makuwa][more]
KAG7014927.12.9e-29691.05Plastidial pyruvate kinase 1, chloroplastic [Cucurbita argyrosperma subsp. argyr... [more]
XP_008454424.18.6e-29690.21PREDICTED: plastidial pyruvate kinase 1, chloroplastic-like [Cucumis melo] >ADN3... [more]
XP_038905899.18.6e-29690.71plastidial pyruvate kinase 1, chloroplastic-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q431171.5e-17058.86Pyruvate kinase isozyme A, chloroplastic OS=Ricinus communis OX=3988 PE=1 SV=1[more]
Q9LIK05.8e-17059.96Plastidial pyruvate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=P... [more]
Q405453.9e-16657.84Pyruvate kinase isozyme A, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Q405461.0e-8639.67Pyruvate kinase isozyme G, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Q9FLW93.0e-8638.24Plastidial pyruvate kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PKP2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7TLC21.1e-29690.38Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G00350... [more]
A0A5D3E0J94.2e-29690.21Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G0035... [more]
E5GBH74.2e-29690.21Pyruvate kinase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1[more]
A0A1S3BYK34.2e-29690.21Pyruvate kinase OS=Cucumis melo OX=3656 GN=LOC103494833 PE=3 SV=1[more]
A0A0A0KSY41.2e-29590.21Pyruvate kinase OS=Cucumis sativus OX=3659 GN=Csa_4G000560 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G22960.14.1e-17159.96Pyruvate kinase family protein [more]
AT5G52920.12.2e-8738.24plastidic pyruvate kinase beta subunit 1 [more]
AT1G32440.11.0e-8439.35plastidial pyruvate kinase 3 [more]
AT3G04050.12.9e-6032.26Pyruvate kinase family protein [more]
AT3G25960.18.6e-6032.99Pyruvate kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001697Pyruvate kinasePRINTSPR01050PYRUVTKNASEcoord: 314..340
score: 52.93
coord: 283..297
score: 47.28
coord: 391..409
score: 54.25
coord: 150..166
score: 27.42
coord: 410..426
score: 35.11
coord: 341..365
score: 48.12
coord: 366..390
score: 57.66
IPR001697Pyruvate kinaseTIGRFAMTIGR01064TIGR01064coord: 93..576
e-value: 2.0E-124
score: 413.7
IPR001697Pyruvate kinasePANTHERPTHR11817PYRUVATE KINASEcoord: 39..577
IPR036918Pyruvate kinase, C-terminal domain superfamilyGENE3D3.40.1380.20coord: 433..559
e-value: 7.8E-141
score: 471.7
IPR036918Pyruvate kinase, C-terminal domain superfamilySUPERFAMILY52935PK C-terminal domain-likecoord: 455..560
IPR015793Pyruvate kinase, barrelPFAMPF00224PKcoord: 92..430
e-value: 3.6E-87
score: 292.5
IPR040442Pyruvate kinase-like domain superfamilyGENE3D3.20.20.60coord: 93..432
e-value: 7.8E-141
score: 471.7
IPR015795Pyruvate kinase, C-terminalPFAMPF02887PK_Ccoord: 460..560
e-value: 2.4E-21
score: 76.1
IPR015806Pyruvate kinase, insert domain superfamilyGENE3D2.40.33.10coord: 161..265
e-value: 7.8E-141
score: 471.7
NoneNo IPR availablePANTHERPTHR11817:SF21PYRUVATE KINASEcoord: 39..577
IPR018209Pyruvate kinase, active sitePROSITEPS00110PYRUVATE_KINASEcoord: 312..324
IPR011037Pyruvate kinase-like, insert domain superfamilySUPERFAMILY50800PK beta-barrel domain-likecoord: 163..249
IPR015813Pyruvate/Phosphoenolpyruvate kinase-like domain superfamilySUPERFAMILY51621Phosphoenolpyruvate/pyruvate domaincoord: 87..433

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc10g03870.1Moc10g03870.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006096 glycolytic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0016301 kinase activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0030955 potassium ion binding
molecular_function GO:0004743 pyruvate kinase activity
molecular_function GO:0003824 catalytic activity